{ "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "FrameKey-0-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "322", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3999cad7-3464-45fa-8e32-91b7c7c3c4bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "323", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2693ed1-6b9f-46e2-9dc4-eeb045760f47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000001__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "324", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "044c8252-5115-4b0b-be03-8758dc6b53f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000002__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "325", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca5f0f3c-2189-46c3-8340-232a1fb4a63c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000003__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "326", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "317841c3-24b3-4ff2-9faf-a2eb39a33019", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000004__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "327", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1796ffe4-f86d-41f9-8b4b-88105e2aeeae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000005__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "328", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a631269-a229-4d97-a1af-4eba09a5e11a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 830.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000006__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 8, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "329", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "8", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c5a5619-e0fc-408a-9d0c-3906d8448e6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 924.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000007__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-8-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 8, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 9, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "330", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "9", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1f612e2-6300-4594-89ba-6184e89704b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 8, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000008__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-9-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 9, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 10, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "331", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "10", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82a83a2a-0fbc-44a6-a81d-ae10e4380f58", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 9, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000009__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-10-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 10, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 11, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "332", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "11", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "456130c1-fc05-44a6-ab2c-cb3dbb7d6bf4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 10, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000010__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-11-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 11, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 12, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "333", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "12", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64c1deab-e0d8-402b-a23a-210f1372e559", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1268.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 11, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000011__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-12-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 12, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 13, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "334", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "13", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22b259cf-e575-4cc1-a3a0-182fe8dfb07b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1361.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 12, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000012__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-13-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 13, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 14, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "335", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "14", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8c8929b-24c3-4429-b1a4-44b129aa7f44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 13, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000013__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-14-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 14, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 15, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "336", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "15", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcb629f3-38e6-47c9-b5cc-86559ffcf433", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 14, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000014__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-15-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 15, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 16, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "337", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "16", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04384b00-a895-48ae-b9b7-8b0824ec8bce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1611.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 15, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000015__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-16-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 16, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 17, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "338", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "17", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "238bcd1b-6ea2-49f2-a11b-140f15afb16c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1705.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 16, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000016__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-17-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 17, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 18, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "339", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "18", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4eedda8-25c2-4cc6-8351-4ab204883a20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 17, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000017__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-18-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 18, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 19, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "340", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "19", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7228e8c4-c3ad-464e-9c4c-0c532f7ac54b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 18, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000018__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-19-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 19, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 20, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "341", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "20", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98affc39-b017-4ef6-ac87-4bd8bbfe6d61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 19, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000019__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-20-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 20, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 21, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "342", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "21", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fdb5518-993a-4e99-a44f-836292299f15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2049.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 20, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000020__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-21-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 21, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "1921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 22, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "343", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "22", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcffdb1a-cfa8-4f7d-8697-50cf3bead91b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2143.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 21, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000021__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-22-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 22, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 23, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "344", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "23", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f6d5801-6178-4009-8330-e985ff7d707a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 22, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000022__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-23-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 23, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 24, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "345", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "24", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e23eaf14-c418-4148-9208-a0086e6bef5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 23, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000023__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-24-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 24, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 25, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "346", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "25", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abb7bcbd-51b3-4453-a1ef-02dba7e3818d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2393.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 24, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000024__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-25-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 25, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 26, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "347", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "26", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d9afb14-6ea7-4d8b-969f-fef8ea7b383d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2486.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 25, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000025__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-26-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 26, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 27, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "348", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "27", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d62065aa-3337-4e77-a81c-46111c184e8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 26, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000026__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-27-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 27, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 28, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "349", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "28", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd09741f-bbf1-47db-a889-a7a61ac49352", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 27, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000027__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-28-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 28, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 29, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "350", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "29", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "348ea36b-7103-4724-aea9-52be8f081b59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 28, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000028__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-29-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 29, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 30, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "351", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "30", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8b9a495-a54e-49e6-9286-729fc916fa7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2830.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 29, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000029__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-30-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 30, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 31, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "352", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "31", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b1acb9a-7efa-4694-9785-66192871ff63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2924.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 30, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000030__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-31-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 31, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 32, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "353", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "32", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b79e11a-b340-4bd2-8b06-3ebcf7c068e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 31, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000031__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-32-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 32, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 33, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "354", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "33", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "847060d6-2a7c-443d-9491-6f93c5350c38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 32, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000032__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-33-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 33, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "2953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 34, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "355", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "34", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22f92139-aad5-4443-98ac-0dc66333f4eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3174.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 33, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000033__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-34-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 34, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 35, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "356", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "35", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2b1f84f-f5df-431f-a59c-dd5970507941", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3268.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 34, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000034__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-35-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 35, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 36, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "357", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "36", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c6bae9a-737a-4fcc-9a7e-c3018a1e73e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 35, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000035__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-36-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 36, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 37, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "358", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "37", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "faf8eb06-7719-4166-a198-e96b0df4f5ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 36, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000036__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-37-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 37, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 38, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "359", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "38", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e53df1fc-e220-40d9-b53b-ef9d730c6e7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3518.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 37, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000037__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-38-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 38, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 39, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "360", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "39", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "318037cd-4a94-43c3-898e-e71d7fef59cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3611.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 38, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000038__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-39-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 39, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 40, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "361", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "40", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb3b39ea-4816-4ad7-8117-cd930c8b5733", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3705.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 39, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000039__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-40-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 40, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 41, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "362", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "41", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c52fbd2c-23c8-4415-8c74-c2e677c98c18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 40, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000040__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-41-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 41, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 42, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "363", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "42", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d79e03d-26ff-42a1-bd05-5637cd114522", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 41, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000041__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-42-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 42, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 43, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "364", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "43", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "610b8de9-891d-46ba-a997-cdca5716d35d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3955.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 42, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000042__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-43-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 43, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 44, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "365", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "44", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b9e1cd0-8df5-4646-9f69-d4c25efe2988", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4049.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 43, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000043__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-44-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 44, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "3906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 45, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "366", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "45", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "296acd5a-07e6-469e-b9ae-3c46a0dbda05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 44, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000044__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-45-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 45, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 46, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "367", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "46", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f99ee9b1-a09a-4a0a-933e-686bb11af075", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 45, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000045__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-46-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 46, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 47, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "368", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "47", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98b9bd21-ff21-4593-997e-40736c4f2753", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4299.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 46, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000046__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-47-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 47, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 48, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "369", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "48", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad907f8e-9ce7-4872-88b6-562f3f7cd278", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4393.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 47, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000047__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-48-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 48, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 49, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "370", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "49", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c948f46-73fe-47ba-aa13-58bd9eca6be6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4486.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 48, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000048__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-49-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 49, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 50, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "371", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "50", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a7f40bd-0ef8-4f8d-b859-008001de8481", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 49, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000049__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-50-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 50, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 51, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "372", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "51", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed6ed373-aa04-479c-85c1-718d4939de8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 50, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000050__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-51-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 51, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 52, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "373", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "52", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "659dd37f-1729-40a1-9bf9-4b42b04c9c2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4736.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 51, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000051__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-52-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 52, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 53, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "374", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "53", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06ed691a-25c0-4985-a745-23a9bae84eaf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4830.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 52, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000052__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-53-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 53, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 54, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "375", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "54", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ceb6d654-8c1c-410f-9752-4b2ed6b7c9d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 53, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000053__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-54-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 54, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 55, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "376", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "55", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e74fd59-8dd9-4944-a7e5-37648d6d5f1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 54, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000054__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-55-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 55, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 56, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "377", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "56", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d55065e-1b0f-4285-b0ce-3f1c1673aa63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5080.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 55, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000055__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-56-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 56, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "4953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 57, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "378", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "57", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37363605-438b-458f-8160-57ef82882992", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5174.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 56, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000056__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-57-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 57, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 58, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "379", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "58", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14819c83-ab0b-444d-a6c8-7e02528457f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 57, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000057__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-58-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 58, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 59, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "380", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "59", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d896f784-670d-4e16-932b-56b84724284a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 58, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000058__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-59-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 59, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 60, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "381", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "60", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ce630e2-6b4c-40d4-a88e-8f213e897061", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 59, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000059__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-60-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 60, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 61, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "382", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "61", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f11b307-bb6f-40bd-b6ef-923851dc9e66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5518.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 60, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000060__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-61-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 61, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 62, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "383", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "62", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f1e6d03-1e95-4ea5-9eea-1895a5892e34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5611.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 61, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000061__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-62-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 62, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 63, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "384", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "63", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28925459-7f5c-4b40-8401-0b69efe943a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 62, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000062__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-63-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 63, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 64, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "385", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "64", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eedbaa3e-6d75-4b56-8546-6e21d0bc1993", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 63, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000063__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-64-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 64, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 65, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "386", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "65", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d92053c1-138d-4a35-8c0c-24a8ce868f90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5861.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 64, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000064__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-65-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 65, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 66, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "387", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "66", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66d88d4d-3516-49b6-b2a2-f447e893d67e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5955.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 65, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000065__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-66-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 66, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 67, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "388", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "67", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d01ae0a-f264-466d-8317-1e9b7fe981ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 66, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000066__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-67-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 67, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "5906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 68, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "389", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "68", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43d19966-1772-4684-a9e0-22c2fa968f4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 67, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000067__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-68-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 68, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 69, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "390", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "69", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7351477-d8b1-4b49-96b4-bbd56b207c08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 68, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000068__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-69-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 69, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 70, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "391", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "70", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c644ffcf-b93c-48ed-9182-86abaad0d93a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6299.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 69, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000069__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-70-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 70, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 71, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "392", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "71", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fa08c01-dd37-4e0e-badd-664e9ff577bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6393.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 70, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000070__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-71-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 71, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 72, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "393", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "72", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fe4db67-57a2-4ee0-b2af-28f4679644bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 71, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000071__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-72-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 72, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 73, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "394", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "73", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccc292e2-c046-4cda-bf6c-b0a5df784dc4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 72, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000072__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-73-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 73, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 74, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "395", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "74", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92a7e0c6-0eee-48fe-aacc-f3ee71e6ddd0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6643.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 73, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000073__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-74-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 74, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 75, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "396", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "75", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b6fe490-c7d2-448b-b88b-64fbab390b53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6736.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 74, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000074__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-75-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 75, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 76, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "397", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "76", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a72029bf-0970-4c6d-8072-f3297954400c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 75, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000075__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-76-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 76, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 77, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "398", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "77", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ebef0f5-b545-4adc-a2cf-28432c996e02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 76, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000076__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-77-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 77, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 78, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "399", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "78", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18606cdd-20a7-4b2f-84a1-ff30cc6a45c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 77, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000077__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-78-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 78, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 79, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "400", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "79", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc42162c-f0ea-4950-812d-029750ba6c3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7080.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 78, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000078__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-79-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 79, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "6953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 80, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "401", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "80", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a976692-232c-4973-b82c-39b8befa366e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7174.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 79, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000079__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-80-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 80, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 81, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "402", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "81", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd6845d9-9689-45ed-b9a4-7f8223048197", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 80, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000080__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-81-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 81, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 82, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "403", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "82", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cb36303-bba9-4112-bafc-75f83c92f77a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 81, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000081__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-82-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 82, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 83, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "404", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "83", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3947f485-d1af-4b68-a235-424f1ede1b78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7424.3699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 82, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000082__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-83-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 83, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 84, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "405", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "84", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "858e0346-ec56-4070-8df6-a2f0268f10a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7518.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 83, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000083__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-84-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 84, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 85, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "406", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "85", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f250f30e-5873-4ee9-a1c2-ff0d7f63a466", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 84, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000084__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-85-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 85, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 86, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "407", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "86", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "647360e0-6f9b-4456-a99f-08a46b6dd587", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 85, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000085__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-86-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 86, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 87, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "408", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "87", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9bc6fff-ec0b-4cf8-bbd2-f87ccb87f981", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7768.1199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 86, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000086__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-87-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 87, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 88, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "409", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "88", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbf306d5-72f3-4b02-b1d5-64a3095ff1d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7861.8699951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 87, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000087__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-88-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 88, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 89, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "410", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "89", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86ffda44-2815-4e4d-a923-460d16210f82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7955.6199951171875, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 88, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000088__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-89-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 89, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 90, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "411", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "90", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f7be863-1ade-432f-8faf-9d0c23bc811f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 89, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000089__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-90-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 90, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 91, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "412", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "91", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f353dd09-b6d7-401d-9293-1b35e1b76af2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 90, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000090__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-91-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 91, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "7984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 92, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "413", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "92", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b96762e-3cbb-42d0-9cfc-a7171541c885", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8205.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 91, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000091__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-92-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 92, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 93, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "414", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "93", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "940e8949-022d-4073-ba07-46d8aec3a61d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8299.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 92, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000092__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-93-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 93, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 94, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "415", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "94", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d56a4138-ebd2-451d-9a1b-865777385c4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 93, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000093__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-94-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 94, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 95, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "416", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "95", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "030833b1-a34a-4731-89e8-b0615181085a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 94, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000094__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-95-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 95, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 96, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "417", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "96", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76380c0f-9b73-413e-9d88-7727c089d19e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8549.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 95, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000095__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-96-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 96, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 97, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "418", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "97", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4693ffde-a672-4e82-b723-c29c166051bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8643.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 96, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000096__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-97-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 97, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 98, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "419", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "98", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4564b7b4-65e7-4da6-ab2a-d1fddd6e002e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8736.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 97, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000097__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-98-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 98, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 99, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "420", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "99", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ae4932c-9d16-490a-b95a-094273e94c7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 98, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000098__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-99-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 99, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 100, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "421", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "100", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c8e1a83-bdd2-4dc1-afde-b02251f30f52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 99, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000099__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-100-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 100, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 101, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "422", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "101", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecd928c4-4a0c-4af7-a2a0-3bd31c168438", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8986.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 100, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000100__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-101-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 101, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 102, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "423", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "102", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23153289-571e-41c9-9233-9f405255bfc3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9080.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 101, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000101__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-102-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 102, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "8937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 103, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "424", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "103", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5eec5511-1c82-44db-bd23-409ff27bcd9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 102, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000102__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-103-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 103, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 104, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "425", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "104", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b795d6f-7bab-4cb8-aace-65756a9dff7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 103, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000103__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-104-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 104, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 105, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "426", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "105", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9823529a-e61a-49dc-9614-d8bee1b1ed09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9330.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 104, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000104__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-105-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 105, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 106, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "427", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "106", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5da80d24-cbd4-4d3e-ba3b-03e48cc5ccd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9424.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 105, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000105__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-106-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 106, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 107, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "428", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "107", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "814bd9b1-10c9-449c-b2ca-6290b743f32f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 106, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000106__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-107-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 107, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 108, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "429", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "108", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c32e97a3-cd72-4a1e-ac6b-b7c10d4546c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 107, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000107__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-108-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 108, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 109, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "430", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "109", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b63aed5a-346f-4af8-af31-041763384ec2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 108, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000108__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-109-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 109, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 110, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "431", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "110", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e5bc5cb-bff5-4f04-b6a0-50e3786eb5ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9768.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 109, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000109__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-110-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 110, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 111, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "432", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "111", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44bbfe95-ab69-4281-ad0e-d1ab88ae1220", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9861.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 110, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000110__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-111-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 111, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 112, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "433", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "112", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "274738be-3ae4-4e60-bed0-b04a1d2c11c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 111, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000111__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-112-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 112, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 113, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "434", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "113", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3dddf5e3-51c5-405c-8e06-d3bb1c12ecf7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 112, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000112__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-113-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 113, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 114, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "435", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "114", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e43285c-6d87-44c6-a720-8e70b84d344e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10111.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 113, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000113__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-114-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 114, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "9984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 115, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "436", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "115", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26e16238-c3a6-49d7-9d6a-e594b13acbcc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10205.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 114, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000114__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-115-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 115, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 116, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "437", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "116", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b88373c8-ae4b-4a41-a67a-642954e02c76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 115, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000115__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-116-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 116, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 117, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "438", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "117", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6da8d3c6-18e4-4121-86be-59fd08d688ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 116, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000116__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-117-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 117, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 118, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "439", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "118", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4b6ef91-350f-4bc4-b6eb-817d29a9ec50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 117, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000117__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-118-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 118, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 119, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "440", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "119", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce0275ab-1ac7-413b-8f5d-0b3213b2b63f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10549.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 118, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000118__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-119-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 119, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 120, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "441", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "120", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b5a9bdb-c819-43af-a2c4-5c1b433b0cfc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10643.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 119, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000119__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-120-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 120, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 121, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "442", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "121", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d76247f-4e0f-4b5e-98ea-0166ecb406a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 120, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000120__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-121-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 121, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 122, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "443", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "122", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d99ecacc-0271-4c4e-b5a4-97ddf6267e51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 121, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000121__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-122-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 122, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 123, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "444", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "123", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22df8cec-fd11-41a7-a9a6-227cefa729e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10893.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 122, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000122__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-123-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 123, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 124, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "445", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "124", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74cd65e7-1387-4260-8044-e0ba890856f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10986.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 123, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000123__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-124-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 124, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 125, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "446", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "125", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "091323f1-b1c8-467c-bf3b-f02c233e6edf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 124, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000124__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-125-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 125, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "10937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 126, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "447", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "126", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9866e53-c59c-4682-a39d-04b32614b75d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 125, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000125__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-126-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 126, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 127, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "448", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "127", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1022da8-0666-4995-b554-d66991292a34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 126, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000126__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-127-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 127, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 128, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "449", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "128", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ba03111-eb1d-4686-9574-25c03f3d86a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11330.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 127, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000127__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-128-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 128, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 129, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "450", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "129", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da1f162c-0314-42e3-ad8f-322726e5819d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11424.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 128, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000128__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-129-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 129, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 130, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "451", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "130", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97983978-c395-4cd7-bfff-b72d2104f3c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 129, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000129__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-130-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 130, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 131, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "452", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "131", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0455eb95-29ce-4a35-aac4-0f8a0c0472f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 130, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000130__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-131-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 131, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 132, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "453", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "132", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e5dab09-66e7-4696-bac5-81a679095405", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11674.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 131, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000131__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-132-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 132, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 133, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "454", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "133", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b189d97c-06d7-4530-890b-b719f7beabe3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11768.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 132, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000132__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-133-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 133, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 134, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "455", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "134", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "534d7904-fe73-47df-8acf-838e759163ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 133, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000133__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-134-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 134, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 135, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "456", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "135", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cb7cdab-6742-488e-b07c-1998c650adf7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 11940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 134, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000134__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-135-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 135, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 136, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "457", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "136", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe94c18f-19d4-4f5c-a9fd-8a4969e0deb3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12018.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 135, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000135__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-136-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 136, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 137, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "458", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "137", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e27f0b34-a337-4306-80cf-431d25979af5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12111.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 136, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000136__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-137-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 137, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "11984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 138, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "459", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "138", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b3d8fcb-101e-4cdb-95c3-19b48c063985", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12205.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 137, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000137__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-138-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 138, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 139, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "460", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "139", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc4cd5a3-da20-48f0-821e-736e96c9ab47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 138, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000138__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-139-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 139, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 140, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "461", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "140", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b631037-5599-466e-94c0-3a8cea6a1ae2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 139, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000139__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-140-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 140, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 141, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "462", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "141", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d1b909b-47ab-4b3a-aa19-1ba0dba36262", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12455.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 140, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000140__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-141-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 141, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 142, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "463", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "142", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "927b6e28-6d47-4664-a558-d474d9181b57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12549.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 141, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000141__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-142-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 142, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 143, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "464", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "143", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "441d65f6-abff-4f14-9edc-7b68aa77bb72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 142, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000142__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-143-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 143, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 144, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "465", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "144", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3f25573-5bf0-42d5-a919-9c307156df45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 143, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000143__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-144-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 144, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 145, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "466", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "145", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5abc5e1d-f46e-4317-949f-181ef29a265a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12799.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 144, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000144__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-145-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 145, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 146, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "467", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "146", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55f82ef5-d4ab-4c32-9307-b46832dd40a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12893.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 145, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000145__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-146-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 146, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 147, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "468", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "147", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be32e8b6-cd02-47a5-ab4b-0a3afd058996", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 12971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 146, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000146__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-147-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 147, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 148, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "469", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "148", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7669d6d1-1aab-4808-98fe-8e47a4147189", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 147, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000147__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-148-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 148, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "12937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 149, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "470", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "149", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c9ead1e-33d0-4f18-ac47-2b80e0525aa2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 148, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000148__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-149-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 149, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 150, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "471", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "150", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7587599-7832-4ea7-b736-8efcc56823de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13236.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 149, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000149__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-150-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 150, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 151, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "472", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "151", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfa2e5f0-07dc-4a38-a00a-788d4b22fa18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13330.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 150, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000150__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-151-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 151, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 152, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "473", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "152", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ece23727-f32b-403b-9216-733b8927c481", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 151, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000151__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-152-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 152, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 153, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "474", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "153", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6e8abf1-769f-42b6-8c6b-6c9d11bc9998", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 152, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000152__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-153-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 153, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 154, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "475", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "154", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4ed62fc-91a8-436b-89cd-78721431d602", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13580.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 153, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000153__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-154-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 154, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 155, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "476", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "155", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94b14ad1-9902-4442-80db-118b4f14dc47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13674.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 154, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000154__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-155-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 155, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 156, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "477", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "156", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed43c14e-40a4-453b-b0a6-dfd83da9eb79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 155, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000155__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-156-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 156, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 157, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "478", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "157", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79052564-117a-44bb-8284-d8c6a926d0a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 156, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000156__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-157-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 157, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 158, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "479", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "158", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71ed67ea-c992-43db-a29d-97a0f5bf24f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 13940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 157, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000157__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-158-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 158, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 159, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "480", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "159", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2c5317d-b649-40e4-83e8-4e17ae6373b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14018.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 158, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000158__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-159-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 159, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 160, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "481", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "160", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56999e52-0fe3-43b3-a0a0-4097979f8334", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14111.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 159, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000159__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-160-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 160, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "13968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 161, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "482", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "161", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "49075c41-5b66-4cbf-adf1-c6831ab0a05c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 160, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000160__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-161-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 161, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 162, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "483", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "162", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccb26f7a-945e-4221-bc56-86907417f102", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 161, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000161__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-162-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 162, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 163, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "484", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "163", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d72e4f7-0c93-44fe-941d-1647d1ae4c01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14361.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 162, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000162__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-163-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 163, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 164, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "485", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "164", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b3b7ea1-b393-411b-862e-ca71906ba9f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14455.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 163, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000163__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-164-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 164, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 165, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "486", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "165", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0930ef5-4828-45ea-9506-9caffaeae5fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 164, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000164__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-165-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 165, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 166, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "487", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "166", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44632046-fefc-4914-8618-a9caf984d1ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 165, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000165__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-166-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 166, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 167, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "488", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "167", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fdfb419c-b6c7-490a-9e5c-8dc856df1ccb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 166, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000166__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-167-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 167, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 168, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "489", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "168", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ef1401e-85c1-4368-a0f1-9c60b3192c90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14799.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 167, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000167__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-168-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 168, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 169, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "490", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "169", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd1d0ec2-89df-486d-8200-7e97ec4ae71a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14893.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 168, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000168__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-169-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 169, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 170, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "491", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "170", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78d9335d-34b6-4631-a44d-f2e82015f747", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 14971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 169, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000169__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-170-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 170, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 171, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "492", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "171", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "078bb65d-599b-4ce5-a3a1-97c66ef11492", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 170, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000170__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-171-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 171, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "14921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 172, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "493", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "172", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d2cab30-f9bb-4e81-827e-9476aaefdafa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15143.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 171, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000171__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-172-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 172, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 173, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "494", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "173", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0514566e-e2b2-4dc4-b519-b8863ab5613b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15236.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 172, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000172__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-173-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 173, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 174, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "495", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "174", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5492909f-b8b9-400b-8a20-fffe6feeea1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 173, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000173__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-174-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 174, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 175, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "496", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "175", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20c7d6b7-b817-44f8-8b67-4b6c21780906", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 174, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000174__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-175-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 175, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 176, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "497", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "176", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c9551eb9-37a2-43bc-ba5b-841fb52bca45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15486.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 175, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000175__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-176-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 176, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 177, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "498", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "177", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "312898f5-f4b6-4bfa-a831-1a320b461c5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15580.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 176, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000176__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-177-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 177, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 178, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "499", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "178", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d77e67b6-4362-4f98-a40e-21b09a4df74c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15674.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 177, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000177__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-178-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 178, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 179, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "500", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "179", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ea242f1-2eb2-48fa-9050-f8b54167cca8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 178, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000178__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-179-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 179, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 180, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "501", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "180", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "796fd277-805a-4013-8809-dd0e2a95f932", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 179, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000179__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-180-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 180, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 181, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "502", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "181", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08351307-fa5b-471c-b850-ddac3ca6b220", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 15924.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 180, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000180__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-181-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 181, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 182, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "503", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "182", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37f77abc-5c54-4b01-aa64-0b6ffd56ee5a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16018.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 181, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000181__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-182-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 182, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 183, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "504", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "183", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea5e0a99-0bdd-4744-8f80-caa987cb85d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 182, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000182__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-183-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 183, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "15968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 184, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "505", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "184", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "981faed6-8f0e-4def-b011-21371c15f7c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 183, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000183__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-184-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 184, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 185, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "506", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "185", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56c78478-6e07-40e1-870f-a792111d8d93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16268.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 184, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000184__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-185-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 185, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 186, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "507", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "186", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42757511-9b4d-4c97-a784-085ba4dcbd8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16361.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 185, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000185__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-186-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 186, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 187, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "508", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "187", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c386092c-becb-40b1-9307-30ce0f460f02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16455.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 186, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000186__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-187-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 187, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 188, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "509", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "188", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "643f4d42-85b4-407a-8e5c-c2add725f727", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 187, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000187__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-188-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 188, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 189, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "510", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "189", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f4d74b2-a74b-498f-b12d-9e1dea562292", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 188, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000188__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-189-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 189, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 190, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "511", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "190", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa32c453-3682-4348-a00b-2e14cbe6cab5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16705.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 189, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000189__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-190-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 190, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 191, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "512", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "191", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b5d745c-1c41-4da7-a1a3-00df7a658346", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16799.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 190, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000190__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-191-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 191, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 192, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "513", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "192", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1fb7c6f-9b92-4e49-8b3c-ce4deffcc725", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 191, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000191__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-192-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 192, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 193, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "514", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "193", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67ffe29c-be14-4f76-86ce-cc0e0fa3ae31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 16971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 192, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000192__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-193-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 193, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 194, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "515", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "194", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff497da7-0858-4fe5-92b0-dd50124a0cb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17049.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 193, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000193__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-194-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 194, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "16921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 195, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "516", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "195", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a04e872f-e99b-45c2-9818-424cb514203e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17143.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 194, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000194__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-195-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 195, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 196, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "517", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "196", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dae831b-fe2e-4c89-b035-4ea717461167", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 195, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000195__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-196-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 196, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 197, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "518", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "197", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca2c7061-c3ca-4408-9f05-e1bd1c2d3b6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 196, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000196__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-197-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 197, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 198, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "519", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "198", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "502fc8f7-f346-4010-83a9-8012e39337c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 197, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000197__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-198-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 198, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 199, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "520", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "199", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57a313e1-045b-4962-9cad-0056291efe5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17486.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 198, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000198__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-199-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 199, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 200, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "521", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "200", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1310eb80-2d9c-4f95-945e-5edcf585e33b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17580.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 199, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000199__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-200-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 200, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 201, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "522", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "201", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5efef0f-464d-4833-b041-bb84005fce54", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 200, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000200__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-201-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 201, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 202, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "523", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "202", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cefbf93-8d92-4609-bf06-d24da7b2ddba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 201, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000201__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-202-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 202, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 203, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "524", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "203", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cef69c1-6fde-4471-94ca-05327b1ef287", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17830.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 202, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000202__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-203-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 203, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 204, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "525", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "204", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12972a6d-3043-4b05-8b01-e091cbe367ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 17924.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 203, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000203__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-204-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 204, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 205, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "526", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "205", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a5cc5a0-6ce6-4b80-8585-0dbc1e83f0c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 204, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000204__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-205-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 205, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 206, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "527", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "206", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07d48d98-e317-4cdf-8a85-6d4749c6d7d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 205, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000205__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-206-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 206, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "17968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 207, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "528", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "207", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ed28098-d463-4c59-917e-2526f03fc02f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 206, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000206__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-207-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 207, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 208, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "529", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "208", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b22aa242-d318-4670-8d20-455e06b43cd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18268.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 207, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000207__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-208-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 208, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 209, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "530", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "209", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af426185-c7e2-4fc0-b677-63f499cf47e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18361.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 208, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000208__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-209-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 209, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 210, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "531", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "210", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f778a245-09cf-44eb-9ca3-f7126a60385d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 209, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000209__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-210-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 210, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 211, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "532", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "211", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa455222-b4ca-4b96-8b68-0c6242f916d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 210, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000210__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-211-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 211, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 212, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "533", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "212", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4366652a-3d0f-458e-bef6-f065ec091390", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18611.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 211, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000211__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-212-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 212, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 213, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "534", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "213", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9abfe9af-ff93-4616-84dc-0612f6f78da3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18705.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 212, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000212__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-213-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 213, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 214, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "535", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "214", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2959477-7e27-4c79-a76c-e29571277ea7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 213, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000213__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-214-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 214, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 215, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "536", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "215", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23415fe2-6a31-483e-8890-b81d08db379a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 214, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000214__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-215-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 215, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 216, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "537", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "216", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8221a4aa-dea5-428d-baee-3b5da9363a83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 18971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 215, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000215__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-216-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 216, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 217, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "538", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "217", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "620c78df-67f3-4f63-a6c6-0bfd4a894f20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19049.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 216, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000216__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-217-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 217, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "18921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 218, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "539", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "218", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "063b7db1-b1d4-4cb0-867d-ea106ab9fbeb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19143.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 217, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000217__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-218-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 218, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 219, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "540", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "219", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2786151-c3a8-47fc-8780-93d7c1ac5e31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 218, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000218__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-219-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 219, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 220, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "541", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "220", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "146f65b8-dbe5-477a-bf41-e5f4f3c1dc4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 219, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000219__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-220-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 220, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 221, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "542", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "221", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1154fcb4-2c52-4839-8de8-03ab6612f5e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19393.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 220, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000220__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-221-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 221, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 222, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "543", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "222", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5c3d366-04e0-4f6d-9bcd-3f50538c4b5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19486.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 221, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000221__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-222-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 222, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 223, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "544", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "223", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8923db51-75ad-483e-b730-c33edb72d452", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 222, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000222__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-223-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 223, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 224, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "545", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "224", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "735c5057-81cb-4739-8891-51c721c68135", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 223, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000223__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-224-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 224, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 225, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "546", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "225", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67d2f1c5-3704-4056-a610-9c83fa5fdd39", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19736.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 224, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000224__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-225-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 225, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 226, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "547", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "226", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7591b4b5-6bd5-4019-8806-9a8e7ed0b690", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19830.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 225, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000225__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-226-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 226, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 227, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "548", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "227", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3ea207f-2f24-4446-87b7-1739f8b76f6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 19924.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 226, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000226__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-227-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 227, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 228, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "549", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "228", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "159e6725-633d-4521-a9f1-e2fcb3d7d07f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 227, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000227__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-228-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 228, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 229, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "550", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "229", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "136e585f-a837-4c4d-857b-b445e1b2e759", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 228, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000228__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-229-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 229, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "19953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 230, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "551", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "230", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6d4b8f4-4cb2-4597-b374-6abff51deadc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20174.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 229, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000229__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-230-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 230, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 231, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "552", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "231", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9863a3cf-fb45-4c87-9dc2-c2219d8d74c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20268.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 230, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000230__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-231-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 231, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 232, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "553", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "232", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94673d4d-a63a-4886-ae2f-5a9e107dad8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 231, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000231__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-232-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 232, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 233, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "554", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "233", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71f42226-3b6a-47cc-9568-a330909eddcf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 232, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000232__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-233-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 233, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 234, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "555", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "234", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "699876d1-3c42-4629-94f5-621c97a43720", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20518.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 233, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000233__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-234-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 234, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 235, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "556", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "235", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9447c5a5-c352-4ab0-8c96-2103463506ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20611.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 234, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000234__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-235-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 235, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 236, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "557", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "236", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c7cdcbd-8811-4f77-ae84-0de30357ca72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20705.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 235, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000235__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-236-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 236, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 237, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "558", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "237", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfb9a35f-607f-44b3-8399-806b0b8fdb99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 236, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000236__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-237-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 237, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 238, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "559", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "238", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "511ab6d3-818f-4f30-88b1-ee3d4fb2ed17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 237, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000237__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-238-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 238, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 239, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "560", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "239", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bb2a1d3-b07b-48e2-977c-b6cc1f95b751", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 20955.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 238, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000238__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-239-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 239, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 240, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "561", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "240", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cab93acc-34e6-43c2-becc-efd0058c4dc6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21049.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 239, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000239__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-240-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 240, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "20906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 241, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "562", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "241", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae9c2d99-9171-4abe-bb73-3edc903d52d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 240, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000240__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-241-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 241, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 242, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "563", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "242", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed5c8053-dc35-4a2e-8f6e-5aa47199078e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 241, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000241__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-242-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 242, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 243, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "564", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "243", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee507562-316d-4bbc-bdd6-698598673990", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21299.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 242, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000242__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-243-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 243, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 244, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "565", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "244", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "887284ac-f318-4c24-97f9-244a78304999", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21393.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 243, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000243__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-244-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 244, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 245, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "566", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "245", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80692989-c0fb-4a26-9569-81fe21fb5e05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 244, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000244__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-245-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 245, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 246, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "567", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "246", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2f00f71-a05e-43c6-830a-617a656eb42a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 245, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000245__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-246-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 246, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 247, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "568", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "247", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19b32bd9-ad3e-4e89-88cb-acbbb41d7be0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 246, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000246__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-247-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 247, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 248, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "569", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "248", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "799b418c-752f-4da8-b58e-08480bfa6135", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21736.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 247, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000247__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-248-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 248, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 249, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "570", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "249", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b40c7deb-9e26-4055-9d25-9a654a593235", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21830.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 248, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000248__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-249-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 249, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 250, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "571", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "250", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a138863-de07-4b12-a6c0-b944b31ad265", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 21908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 249, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000249__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-250-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 250, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 251, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "572", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "251", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "163c0a69-59dd-4a57-82d4-d661aef54354", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 250, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000250__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-251-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 251, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 252, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "573", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "252", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "982da984-73a6-430e-b9ba-f8b83f2f7df6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22080.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 251, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000251__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-252-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 252, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "21953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 253, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "574", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "253", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2da68539-0243-4e87-b0d4-bb814bafb0e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22174.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 252, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000252__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-253-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 253, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 254, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "575", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "254", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d45bec1-1064-45bb-a56b-0676ba7477f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 253, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000253__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-254-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 254, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 255, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "576", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "255", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09be2f58-6781-4cf1-9558-55100e15c1de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 254, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000254__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-255-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 255, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 256, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "577", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "256", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96b6d890-05d1-483b-b71c-1acef138c49c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 255, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000255__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-256-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 256, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 257, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "578", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "257", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94813f00-98e8-423e-9e83-a06fa67735e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22518.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 256, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000256__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-257-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 257, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 258, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "579", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "258", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b643551-f65a-4452-a05c-bcfb45eee32b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22611.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 257, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000257__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-258-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 258, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 259, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "580", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "259", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bcecd9f-6636-407b-b668-25264809d41f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 258, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000258__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-259-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 259, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 260, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "581", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "260", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10b0ac02-1738-4f8e-a93c-5fa7724ca59d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 259, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000259__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-260-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 260, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 261, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "582", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "261", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8986ac3-a064-4d15-bf32-d92a31a5a443", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22861.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 260, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000260__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-261-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 261, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 262, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "583", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "262", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86e49c43-9530-4fc0-a1c6-3b8d44f7b040", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 22955.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 261, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000261__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-262-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 262, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 263, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "584", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "263", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30a01791-cd50-4c4f-b9f1-5365b4ebbdb5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 262, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000262__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-263-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 263, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "22906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 264, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "585", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "264", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ae6abc2-7ad6-4ad1-9398-f79cd00fa648", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 263, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000263__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-264-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 264, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 265, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "586", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "265", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6240ae49-01d6-40fa-adca-64d37169730f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 264, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000264__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-265-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 265, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 266, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "587", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "266", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7628f85-c555-472b-8654-80c7958b20da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23299.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 265, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000265__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-266-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 266, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 267, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "588", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "267", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8529fad6-9f8b-4883-9734-e15edac02a97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23393.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 266, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000266__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-267-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 267, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 268, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "589", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "268", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62d3545c-2627-403c-8ae5-0c371a680878", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 267, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000267__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-268-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 268, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 269, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "590", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "269", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b2c8ddf-31df-4731-9ba0-b3b1279f850d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 268, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000268__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-269-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 269, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 270, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "591", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "270", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44599c01-526a-464c-8191-c84d5f60255a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23643.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 269, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000269__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-270-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 270, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 271, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "592", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "271", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06d48cef-83e9-47be-9a83-2d8d678ddcd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23736.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 270, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000270__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-271-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 271, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 272, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "593", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "272", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdf99c86-4859-4bfb-9085-a511d677a075", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 271, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000271__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-272-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 272, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 273, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "594", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "273", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56da3123-f2a2-4f2a-b748-cca02d52e3b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 272, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000272__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-273-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 273, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 274, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "595", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "274", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af8211b2-22c7-4ec0-834a-b66052eee6fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 23986.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 273, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000273__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-274-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 274, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 275, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "596", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "275", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "407dda99-9f1e-4fad-a99c-13c54410bbc9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24080.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 274, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000274__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-275-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 275, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "23953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 276, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "597", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "276", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d63105df-9e67-49ce-8160-29a5e3684b83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24174.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 275, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000275__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-276-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 276, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 277, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "598", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "277", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cdb6599e-76fd-4f15-b81d-16edfc542479", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 276, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000276__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-277-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 277, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 278, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "599", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "278", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e666ad22-569c-4208-a4bf-2ca6eb51b6a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 277, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000277__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-278-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 278, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 279, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "600", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "279", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fb698b1-3c85-4f69-b96b-c0ece243ebc9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24424.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 278, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000278__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-279-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 279, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 280, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "601", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "280", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "112c20e6-bdcd-48c7-b392-a562b1e5b4dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24518.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 279, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000279__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-280-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 280, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 281, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "602", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "281", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70fbff5c-86bd-4922-b4d8-283e3b8bc478", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 280, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000280__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-281-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 281, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 282, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "603", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "282", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "794b9366-cce0-41bb-b3b0-a03e7258ecd4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 281, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000281__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-282-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 282, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 283, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "604", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "283", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5966eb24-7bb7-4e6c-86e7-9ab1e51dedfb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24768.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 282, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000282__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-283-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 283, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 284, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "605", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "284", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b2841fc-db1a-4b77-a747-d7902f6fa836", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24861.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 283, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000283__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-284-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 284, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 285, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "606", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "285", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce76d23e-33df-4966-8d7e-c10d6c5531de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 24955.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 284, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000284__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-285-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 285, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 286, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "607", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "286", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a14488d4-a0cc-4ee9-9b32-e9203b5238aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 285, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000285__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-286-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 286, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 287, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "608", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "287", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bed204bf-ec03-4d24-97aa-00dba1b23a2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 286, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000286__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-287-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 287, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "24984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 288, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "609", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "288", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5954beab-1e5a-4694-ac6b-e2b2a7e6413f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25205.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 287, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000287__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-288-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 288, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 289, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "610", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "289", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9881f0fa-0284-422f-9301-fccef6433c8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25299.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 288, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000288__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-289-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 289, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 290, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "611", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "290", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f91d3c6c-1bb9-4a5e-8f86-5b04ababea22", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 289, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000289__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-290-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 290, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 291, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "612", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "291", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3c1cdb2-3cb8-4efc-afa2-f5f29d4ef4aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 290, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000290__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-291-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 291, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 292, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "613", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "292", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5a25760-7755-40a6-9965-e04e9c3222de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25549.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 291, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000291__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-292-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 292, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 293, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "614", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "293", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e589d42-5ce2-42bc-8c56-2f28782eb31c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25643.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 292, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000292__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-293-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 293, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 294, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "615", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "294", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2de2468-f65f-4ed8-92d1-9fb2aeecd2bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25736.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 293, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000293__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-294-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 294, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 295, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "616", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "295", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56dafdc1-73d0-4a4b-b7be-81ed95e3446b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 294, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000294__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-295-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 295, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 296, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "617", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "296", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f0913b3-1dac-42fd-b486-c8624c54cd2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 295, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000295__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-296-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 296, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 297, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "618", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "297", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1be317e-2b80-48df-8ecf-54dbf2c20ea9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 25986.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 296, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000296__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-297-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 297, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 298, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "619", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "298", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "134c6e77-ec02-4334-a833-50a195b22e65", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26080.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 297, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000297__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-298-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 298, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "25937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 299, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "620", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "299", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e268a22-bf39-41c6-a2bb-c75a877685b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 298, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000298__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-299-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 299, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 300, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "621", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "300", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3386ff9a-20e7-4193-9e7f-ea1b9587279c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 299, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000299__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-300-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 300, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 301, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "622", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "301", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6a61705-8845-4c79-b1b8-4627ea600ea4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26330.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 300, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000300__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-301-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 301, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 302, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "623", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "302", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abac84ab-93ce-4d1b-9ac0-4145c46108ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26424.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 301, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000301__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-302-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 302, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 303, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "624", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "303", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af5beebb-a785-421e-b9cf-4a39febf96db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 302, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000302__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-303-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 303, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 304, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "625", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "304", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cbea5f5c-28d6-4abb-9c0c-9b226f79ecb3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 303, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000303__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-304-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 304, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 305, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "626", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "305", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "952072c7-7753-45fb-b310-4aca1f17fd72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 304, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000304__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-305-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 305, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 306, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "627", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "306", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e34eea5-5cb9-4079-b269-5094ae369d9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26768.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 305, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000305__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-306-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 306, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 307, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "628", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "307", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc9870c4-2877-4942-b0b0-9078db3016fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26861.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 306, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000306__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-307-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 307, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 308, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "629", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "308", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd1eaa6b-b737-4330-adc1-7c53de87fa5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 26940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 307, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000307__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-308-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 308, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 309, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "630", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "309", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e200600-d0bf-499f-8724-c666d23b161b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 308, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000308__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-309-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 309, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 310, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "631", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "310", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8500105-da14-4cc8-9ff6-32dc44ec6dcf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27111.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 309, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000309__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-310-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 310, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "26984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 311, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "632", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "311", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bf4cf52-63cb-4eff-9bf1-7053f5599c7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27205.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 310, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000310__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-311-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 311, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 312, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "633", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "312", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a8925bb1-5d28-4faf-b744-db60c77ec553", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 311, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000311__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-312-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 312, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 313, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "634", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "313", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "181ad33b-a05b-4b73-9ba9-6dda5864d4fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 312, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000312__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-313-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 313, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 314, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "635", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "314", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8f3db0b-c460-4a1b-b64e-8f9319fd55d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 313, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000313__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-314-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 314, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 315, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "636", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "315", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1615d72-3f8f-49b6-8823-89c7e36ad88b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27549.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 314, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000314__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-315-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 315, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 316, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "637", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "316", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "684de828-d732-4bf3-ac50-28cabf0a02c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27643.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 315, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000315__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-316-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 316, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 317, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "638", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "317", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef8c4a59-89e5-4476-b62b-cccbdf31861d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 316, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000316__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-317-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 317, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 318, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "639", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "318", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0eecec62-c7a9-4291-8a26-24683b3511c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 317, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000317__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-318-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 318, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 319, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "640", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "319", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d607fc91-1f13-448a-8bb3-bc430da98d4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27893.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 318, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000318__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-319-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 319, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 320, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "641", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "320", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "543a68a3-8737-45ca-9387-ff8484d3228a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 27986.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 319, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000319__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-320-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 320, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 321, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "642", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "321", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e6931f5-6033-417a-bc56-aab46ae19995", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 320, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000320__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-321-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 321, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "27937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 322, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "643", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "322", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0763150-9b49-4529-9098-ffc03368dcaf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 321, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000321__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-322-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 322, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 323, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "644", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "323", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ade2c2cb-94e5-4c71-92ab-f0d5a379699b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28236.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 322, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000322__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-323-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 323, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 324, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "645", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "324", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46e40e12-9642-445e-a663-5563c8ae9f7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28330.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 323, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000323__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-324-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 324, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 325, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "646", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "325", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc3bb8a2-28ca-4e50-9889-272974bab120", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28424.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 324, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000324__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-325-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 325, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 326, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "647", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "326", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca67c320-ce85-4274-94af-536faebd799a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 325, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000325__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-326-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 326, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 327, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "648", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "327", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e8ca088-6a46-4813-83b8-81dbfe60180f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 326, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000326__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-327-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 327, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 328, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "649", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "328", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "edbef363-2ef8-4d3c-aea8-1fed99c93f2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28674.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 327, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000327__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-328-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 328, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 329, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "650", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "329", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aeacb88f-cf7f-4c28-9664-4451b7f7ef37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28768.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 328, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000328__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-329-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 329, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 330, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "651", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "330", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3983e591-af78-42ec-a077-5d7bbe20c125", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 329, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000329__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-330-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 330, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 331, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "652", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "331", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4689d16-a38f-4ec4-a44f-9e4616552171", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 28940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 330, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000330__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-331-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 331, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 332, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "653", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "332", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71836c81-96e8-4b91-af72-0fced9f1765f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29018.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 331, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000331__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-332-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 332, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 333, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "654", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "333", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8146fa2d-576c-4304-95f9-020af2576650", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29111.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 332, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000332__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-333-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 333, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "28984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 334, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "655", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "334", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "054bad67-6bc2-4e22-bf53-41453997846f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29205.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 333, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000333__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-334-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 334, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 335, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "656", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "335", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcb69f94-2a30-4890-8d77-d19f3a499318", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 334, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000334__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-335-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 335, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 336, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "657", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "336", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4374177a-b742-45f8-9928-0bfcce753205", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 335, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000335__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-336-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 336, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 337, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "658", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "337", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04928812-c3eb-4536-9e1f-eca8b6e87c20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29455.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 336, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000336__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-337-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 337, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 338, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "659", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "338", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db497ef8-8038-41da-a7c8-d592fd512ee0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29549.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 337, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000337__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-338-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 338, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 339, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "660", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "339", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3329f886-a2fb-43f9-a3d0-24a60a5f230b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 338, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000338__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-339-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 339, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 340, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "661", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "340", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80c11d3a-9748-46ad-a78b-bcbf050b2094", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 339, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000339__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-340-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 340, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 341, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "662", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "341", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "deff40d7-804a-4ee3-a2d7-8e70a83fd084", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29799.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 340, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000340__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-341-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 341, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 342, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "663", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "342", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dee023ba-f695-4f78-8163-f0208bdaa107", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29893.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 341, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000341__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-342-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 342, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 343, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "664", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "343", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1074e3cd-a751-4a25-b088-40b47857324e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 29986.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 342, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000342__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-343-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 343, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 344, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "665", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "344", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b6d449f-54dd-42b2-bd71-fa7a10f63e33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 343, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000343__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-344-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 344, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "29937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 345, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "666", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "345", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c5e8e74-b8af-4808-9773-6a2b895504b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 344, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000344__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-345-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 345, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 346, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "667", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "346", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f3a2fd3-e2bc-441f-9a68-e869da45756f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30236.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 345, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000345__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-346-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 346, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 347, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "668", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "347", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4da3e5e2-bb2f-4625-b320-4fc969667c88", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30330.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 346, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000346__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-347-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 347, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 348, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "669", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "348", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5534088-8c3a-4e8b-91da-5f15f3a93846", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 347, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000347__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-348-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 348, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 349, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "670", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "349", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d844deaa-842e-42b3-979a-5fece0558f1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 348, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000348__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-349-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 349, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 350, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "671", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "350", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2945ac0d-ef23-491d-b73b-b4f7aacb0bfe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30580.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 349, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000349__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-350-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 350, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 351, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "672", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "351", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "636d1a36-b32b-4692-ab6d-78e354f0216a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30674.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 350, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000350__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-351-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 351, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 352, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "673", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "352", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "decfc78c-541a-4093-8f02-6d75cfd9edae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 351, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000351__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-352-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 352, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 353, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "674", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "353", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82ac5685-0a57-4bcc-9669-4553e2ed0854", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 352, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000352__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-353-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 353, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 354, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "675", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "354", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40eeb669-8ad4-417d-9625-5b3c181e1218", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 30940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 353, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000353__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-354-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 354, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 355, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "676", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "355", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3ad8339-e208-4be7-9877-4d5a577bc5ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31018.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 354, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000354__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-355-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 355, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 356, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "677", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "356", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4910e60b-79a4-41fa-8850-55d181fa0c47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31111.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 355, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000355__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-356-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 356, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "30968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 357, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "678", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "357", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1125559f-e305-485c-844e-7dd61f932820", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 356, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000356__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-357-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 357, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 358, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "679", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "358", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e4b472e-d661-43fc-b7ab-7e000591a42e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 357, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000357__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-358-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 358, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 359, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "680", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "359", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "609461f6-d9ce-494d-9145-9a719f711a11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31361.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 358, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000358__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-359-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 359, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 360, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "681", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "360", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4d7dfc6-e8c7-4623-9749-0bf06c89032b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31455.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 359, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000359__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-360-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 360, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 361, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "682", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "361", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98165256-3655-45ec-938b-18de1580b488", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 360, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000360__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-361-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 361, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 362, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "683", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "362", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd7c4632-77d1-48d1-ac04-199c34fb0475", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 361, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000361__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-362-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 362, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 363, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "684", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "363", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2852a4f3-74b8-427d-88e5-fd34aa8e8270", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 362, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000362__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-363-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 363, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 364, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "685", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "364", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3257d0b8-5aa4-433c-a600-368440cf185f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31799.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 363, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000363__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-364-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 364, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 365, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "686", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "365", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "854e4cb1-7144-4289-b48c-ef1fe990cd31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31893.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 364, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000364__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-365-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 365, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 366, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "687", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "366", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f8974dd-64f3-4922-9276-c09073771527", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 31971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 365, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000365__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-366-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 366, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 367, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "688", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "367", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf457b85-381f-47be-ad54-111f9ceb13e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 366, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000366__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-367-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 367, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "31921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 368, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "689", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "368", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9c604f5-aa7d-4ac5-9784-7f55046aec0e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32143.119995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 367, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000367__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-368-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 368, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 369, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "690", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "369", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "042cbe6f-6e11-424e-9441-20df69e3b0b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32236.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 368, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000368__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-369-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 369, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 370, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "691", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "370", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89711865-3b94-4db4-a481-4453b90b4f73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 369, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000369__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-370-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 370, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 371, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "692", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "371", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1f7c2a4-940c-42d3-a16d-036ba360e17e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 370, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000370__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-371-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 371, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 372, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "693", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "372", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "330f109e-1456-4096-8699-3b5fcc3c1cac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32486.869995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 371, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000371__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-372-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 372, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 373, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "694", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "373", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "902428cd-a045-46f0-80cd-21c7cc85c6df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32580.619995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 372, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000372__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-373-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 373, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 374, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "695", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "374", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82afbeff-1bcc-4947-87d0-62ca81c0a120", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32674.369995117188, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 373, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000373__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-374-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 374, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 375, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "696", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "375", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58100ec9-c844-470f-9dc8-fbf605019694", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 374, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000374__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-375-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 375, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 376, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "697", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "376", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d1509fd-0422-4a2b-ace6-ccdf4e2f20e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 375, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000375__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-376-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 376, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 377, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "698", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "377", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72d54bf8-cf28-43c0-9953-a461a9412458", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 32924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 376, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000376__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-377-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 377, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 378, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "699", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "378", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0593c937-5baa-48c7-9f9c-687157ae3700", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 377, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000377__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-378-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 378, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 379, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "700", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "379", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6163c453-b381-4904-a5e7-64fa3bb6211d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 378, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000378__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-379-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 379, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "32968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 380, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "701", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "380", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f6b14ff-202f-4811-8be6-0dd3a8b1ae9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 379, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000379__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-380-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 380, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 381, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "702", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "381", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fea5b64-5d8e-4f98-b285-5a8f3da18334", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 380, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000380__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-381-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 381, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 382, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "703", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "382", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad25c530-f300-4a7b-a3c6-74978c9035d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 381, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000381__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-382-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 382, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 383, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "704", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "383", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eff4bc26-27e3-450c-acbb-caabae57794e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 382, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000382__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-383-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 383, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 384, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "705", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "384", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9bc6dac-ceca-430f-a2c6-e48e57385b01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 383, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000383__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-384-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 384, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 385, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "706", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "385", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4651eca-8ebc-472d-9061-4ba35e6c0ee8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 384, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000384__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-385-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 385, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 386, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "707", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "386", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9889e1f-ef34-4c16-a52d-8062f9a63be3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 385, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000385__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-386-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 386, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 387, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "708", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "387", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74424bfc-ded4-4522-9ec7-e6a41bcc46cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 386, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000386__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-387-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 387, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 388, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "709", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "388", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a98e875e-143c-45a5-a3b0-c8279c5848a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 387, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000387__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-388-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 388, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 389, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "710", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "389", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "252da3f0-0fb3-499f-a141-e539c5d57338", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 33971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 388, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000388__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-389-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 389, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 390, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "711", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "390", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "344bd541-e269-4c0f-b789-e101e4f3b379", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 389, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000389__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-390-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 390, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "33921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 391, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "712", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "391", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5d8410f-3aa9-4b59-90f0-c0afbe0e9a68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 390, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000390__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-391-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 391, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 392, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "713", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "392", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7eabfb60-4b01-4cc6-94ae-732757ea7052", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 391, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000391__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-392-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 392, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 393, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "714", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "393", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6aa990f1-5264-4105-93c5-dbb75b6bd75f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 392, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000392__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-393-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 393, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 394, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "715", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "394", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "320f5254-4efb-4653-9296-5a631c95d39e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 393, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000393__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-394-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 394, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 395, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "716", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "395", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f309d83-417e-44e0-af0b-614f68e2277a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 394, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000394__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-395-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 395, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 396, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "717", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "396", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6698ac0-665b-483a-9ef9-afdaedb5ab40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 395, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000395__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-396-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 396, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 397, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "718", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "397", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbaf10db-6d2a-41f5-a69f-eebaa6f4f4ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 396, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000396__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-397-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 397, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 398, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "719", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "398", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81a9b2d8-3f19-435b-b0af-09bd0881705d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 397, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000397__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-398-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 398, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 399, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "720", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "399", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "348d0c22-cca5-4cba-95c8-3f7587445b80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 398, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000398__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-399-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 399, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 400, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "721", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "400", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "103e54ac-5ffe-437e-9e13-f829804cfe20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 34924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 399, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000399__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-400-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 400, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 401, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "722", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "401", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0aaaf3f7-6162-4299-b5a1-b228615cc215", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 400, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000400__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-401-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 401, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 402, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "723", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "402", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a145b199-c188-40f4-b245-05437ee8a56f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 401, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000401__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-402-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 402, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "34968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 403, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "724", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "403", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a376f12-49c4-4abd-b92e-ff9a20da170d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 402, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000402__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-403-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 403, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 404, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "725", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "404", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a8b721b0-2830-42c4-867c-864ffea74ee6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 403, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000403__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-404-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 404, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 405, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "726", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "405", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0176d2a7-2d59-45ad-8c74-c136bd4be7a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 404, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000404__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-405-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 405, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 406, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "727", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "406", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54fafcc6-b247-4c79-834e-36282a939328", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 405, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000405__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-406-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 406, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 407, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "728", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "407", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1741c076-86a3-42aa-a4b2-9dbd32be5533", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 406, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000406__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-407-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 407, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 408, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "729", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "408", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b445cfb2-a9f0-47dc-bc07-43cb8e159252", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 407, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000407__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-408-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 408, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 409, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "730", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "409", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c210899-79fe-4bcc-8f24-d8c3980f0110", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 408, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000408__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-409-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 409, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 410, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "731", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "410", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d063002-7b82-42ce-b0d7-3579fe434385", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 409, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000409__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-410-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 410, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 411, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "732", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "411", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a4d4ab1-7afd-4d1f-97e9-26cd4d53be5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 410, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000410__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-411-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 411, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 412, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "733", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "412", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a4fad0c-4173-4b00-aba7-e6fb2c6b0b3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 35971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 411, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000411__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-412-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 412, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 413, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "734", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "413", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d22dd2f4-c0de-4899-8941-97299807e89d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 412, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000412__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-413-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 413, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "35921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 414, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "735", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "414", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4faf9c25-b960-428a-a122-48c396325591", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 413, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000413__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-414-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 414, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 415, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "736", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "415", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f31624d-52cb-41d6-8789-e357569669c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 414, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000414__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-415-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 415, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 416, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "737", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "416", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cab820c-24c9-46b4-83c8-856730a7faab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 415, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000415__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-416-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 416, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 417, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "738", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "417", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69d1720e-63a5-47c8-bde6-c26ab0e07bec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 416, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000416__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-417-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 417, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 418, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "739", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "418", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae146360-5889-4f3a-acd1-be84727bfc12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 417, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000417__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-418-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 418, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 419, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "740", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "419", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5f5b857-8eec-430e-8f9a-4fc27d7c22e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 418, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000418__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-419-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 419, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 420, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "741", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "420", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f96e7fc-0c0b-4af6-8674-115d0c0a5a35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 419, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000419__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-420-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 420, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 421, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "742", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "421", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e180d440-e6f1-4d26-9bfc-25b216c1564a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 420, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000420__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-421-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 421, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 422, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "743", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "422", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12eeacb1-8933-4d9b-a9b9-3255557dfd4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 421, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000421__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-422-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 422, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 423, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "744", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "423", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14aa1b66-2bae-4916-81fe-5f49c69bf68a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 36924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 422, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000422__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-423-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 423, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 424, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "745", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "424", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "905638b5-e96e-4679-bbf6-1072fe6c0b55", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 423, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000423__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-424-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 424, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 425, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "746", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "425", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25455a22-439d-46ff-a53b-dae887da7bb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 424, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000424__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-425-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 425, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "36953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 426, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "747", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "426", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98b48abd-72fe-4a85-b5ca-8eb7a9503dea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 425, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000425__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-426-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 426, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 427, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "748", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "427", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a61cce7b-eda3-4329-bee3-0ce673323602", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 426, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000426__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-427-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 427, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 428, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "749", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "428", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8022dadf-9ebb-4a8d-a2ff-8cc360583e33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 427, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000427__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-428-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 428, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 429, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "750", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "429", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b7ce66c1-494a-4b5a-a662-f4f44386f2a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 428, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000428__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-429-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 429, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 430, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "751", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "430", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6532efd-bf10-4b07-9e75-fa0f268ea106", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 429, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000429__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-430-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 430, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 431, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "752", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "431", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec9045f1-0e6c-44f9-97a5-c6b4f06ee2af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 430, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000430__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-431-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 431, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 432, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "753", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "432", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c73749d-1bdb-4e14-bece-d34ce5516c03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 431, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000431__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-432-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 432, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 433, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "754", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "433", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "532e9d5d-e37f-43c4-8afb-3511cff53191", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 432, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000432__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-433-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 433, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 434, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "755", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "434", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4a4c613-1be6-4cc8-9753-7efbf1f46e78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 433, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000433__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-434-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 434, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 435, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "756", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "435", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ad161e7-3449-4486-b454-9e8279da52f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 37955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 434, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000434__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-435-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 435, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 436, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "757", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "436", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0db2b93c-c106-4506-87f9-5210a80ffe0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 435, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000435__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-436-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 436, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "37906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 437, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "758", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "437", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b2f2c7b-20b4-49af-90f0-f9366c5f5a5c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 436, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000436__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-437-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 437, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 438, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "759", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "438", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27b361b5-413a-45b8-84e5-721efd3ec9b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 437, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000437__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-438-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 438, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 439, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "760", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "439", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41233eac-8e22-4488-b3e5-8b9ad09d99b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 438, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000438__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-439-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 439, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 440, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "761", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "440", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac15506e-1754-4ca7-885c-f3e281c2dcec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 439, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000439__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-440-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 440, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 441, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "762", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "441", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d465b4e4-374a-4724-8e4e-a2708913f6f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 440, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000440__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-441-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 441, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 442, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "763", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "442", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "def54c43-d700-4784-a12d-9ea93e1dbd8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 441, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000441__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-442-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 442, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 443, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "764", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "443", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3790d05e-b072-4b14-936e-4496be8e2c93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 442, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000442__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-443-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 443, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 444, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "765", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "444", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d531fa14-0aee-46f4-b189-1a455c861f41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 443, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000443__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-444-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 444, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 445, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "766", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "445", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "184e7f60-119d-46c6-a9ee-5c5ebbaa623f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 444, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000444__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-445-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 445, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 446, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "767", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "446", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44d260cf-10ec-47d2-9fae-55075eae5015", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 38908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 445, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000445__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-446-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 446, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 447, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "768", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "447", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab549912-b24b-472a-8b1a-c67cb4acf0fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 446, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000446__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-447-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 447, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 448, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "769", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "448", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c79a0c3-a8da-437e-b7bf-306626e2db13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 447, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000447__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-448-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 448, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "38953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 449, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "770", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "449", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bad4d70-2c55-49fc-9e5c-ff3d867ee7c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 448, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000448__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-449-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 449, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 450, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "771", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "450", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76c194d5-9d78-4610-af03-1c483c459809", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 449, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000449__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-450-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 450, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 451, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "772", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "451", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ff42e65-53f7-4871-b3df-87dda721dfd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 450, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000450__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-451-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 451, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 452, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "773", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "452", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17bed9da-c2d6-4c53-b431-75d7b8c1da98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 451, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000451__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-452-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 452, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 453, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "774", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "453", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b55d9df1-21cc-43f6-838b-eb8d1afe8778", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 452, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000452__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-453-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 453, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 454, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "775", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "454", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1927009f-072a-42e7-b0cc-d4664f5911f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 453, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000453__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-454-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 454, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 455, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "776", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "455", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbc74d7d-4361-4428-9d72-f4612434744d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 454, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000454__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-455-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 455, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 456, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "777", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "456", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "820ec81a-cb0a-49cf-acf0-16681174b698", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 455, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000455__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-456-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 456, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 457, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "778", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "457", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3cc470d-37b1-4554-a2bc-e94fd70db2d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 456, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000456__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-457-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 457, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 458, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "779", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "458", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3f2050f-612a-4c6e-a6b4-bf1b2298cd41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 39955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 457, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000457__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-458-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 458, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 459, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "780", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "459", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c948bcf1-1bc8-4754-9655-4a054ae610f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 458, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000458__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-459-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 459, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "39906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 460, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "781", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "460", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "011196c6-051f-4e42-a721-b315f6f3f589", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 459, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000459__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-460-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 460, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 461, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "782", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "461", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b447a85-2ec9-45ae-9c25-753739a5ab46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 460, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000460__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-461-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 461, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 462, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "783", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "462", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cdcd9c98-f778-40e9-aadf-08f69a115948", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 461, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000461__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-462-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 462, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 463, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "784", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "463", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06f5fa1e-91ed-48f0-bb47-1d912fb9b718", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 462, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000462__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-463-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 463, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 464, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "785", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "464", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0cf1644-73a9-4605-bd3c-58516352744b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 463, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000463__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-464-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 464, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 465, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "786", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "465", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f36d41c-d72a-497d-a30e-6dd83a4cb74b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 464, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000464__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-465-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 465, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 466, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "787", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "466", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3612c705-5426-4dcd-bd71-c8d43be0b90a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 465, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000465__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-466-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 466, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 467, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "788", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "467", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03956401-a68d-4268-ba36-689da01dc42c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 466, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000466__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-467-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 467, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 468, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "789", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "468", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db2e2496-64d3-485d-a702-188764d76690", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 467, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000467__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-468-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 468, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 469, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "790", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "469", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "536350c3-1649-4af2-a905-23ca8e06d292", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 40908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 468, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000468__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-469-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 469, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 470, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "791", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "470", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9410e5d-528e-4b6e-a19b-2414ea569e4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 469, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000469__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-470-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 470, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 471, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "792", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "471", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a3e115b-1e49-4a0a-9e4f-ca628f8bee97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 470, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000470__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-471-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 471, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "40953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 472, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "793", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "472", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5752e1a-804b-4527-844b-c5d2ae6b418e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 471, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000471__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-472-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 472, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 473, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "794", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "473", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd4f97a2-fef0-4455-94f2-6e1ab351bf31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 472, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000472__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-473-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 473, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 474, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "795", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "474", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59d26f14-26c1-4bf1-bec5-f146221c5100", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 473, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000473__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-474-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 474, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 475, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "796", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "475", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5ad09be-7bc6-42f6-ae30-743d5787d8ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 474, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000474__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-475-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 475, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 476, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "797", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "476", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9be551e-862b-4358-86f9-aef15abc1c62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 475, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000475__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-476-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 476, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 477, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "798", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "477", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e5e4827-38be-4580-bc6d-4a3c7bbf4496", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 476, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000476__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-477-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 477, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 478, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "799", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "478", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef2908fd-4bd9-4e1b-88cc-754ff8b4b56a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 477, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000477__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-478-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 478, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 479, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "800", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "479", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1171d5b-c44b-4b35-bc4a-97b8f791e289", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 478, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000478__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-479-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 479, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 480, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "801", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "480", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac97aaf2-3dcd-4502-bc24-00002e38ca2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 479, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000479__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-480-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 480, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 481, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "802", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "481", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99f4a80d-a86f-4944-ac6a-8e70a30f5bf0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 41955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 480, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000480__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-481-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 481, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 482, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "803", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "482", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4527f229-45f7-4a1e-bc75-d1ca335cc1af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 481, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000481__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-482-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 482, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 483, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "804", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "483", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db9d9c25-89a1-44fa-b06f-f0c85f2f4c4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 482, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000482__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-483-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 483, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "41984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 484, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "805", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "484", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58f9f230-a6f1-4f09-93c6-d43a5517eb7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 483, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000483__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-484-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 484, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 485, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "806", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "485", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55735bf1-432f-47d5-a518-dc36456bc2f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 484, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000484__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-485-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 485, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 486, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "807", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "486", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7b0fd09-9180-4d9d-b701-521655a2fd41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 485, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000485__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-486-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 486, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 487, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "808", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "487", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c53c67c-d4ee-4b9f-a5e5-e0f72da35ec4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 486, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000486__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-487-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 487, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 488, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "809", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "488", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9dae7d1-d6c0-4264-a960-969648773b53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 487, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000487__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-488-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 488, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 489, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "810", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "489", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a407c69e-8a9f-49e1-92f1-c5a39f48c18d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 488, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000488__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-489-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 489, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 490, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "811", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "490", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb3ab245-fc80-4dca-a71e-428a7e2a5010", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 489, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000489__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-490-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 490, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 491, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "812", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "491", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00353001-e563-4143-b205-edb4b69febe2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 490, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000490__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-491-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 491, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 492, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "813", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "492", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d1f58fa-9e89-4633-974d-13664827bb4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 491, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000491__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-492-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 492, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 493, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "814", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "493", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10ea73d3-a204-4e8e-a96e-f676970a8311", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 42986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 492, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000492__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-493-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 493, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 494, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "815", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "494", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "284b79b6-2e45-4308-afc0-8cab09b1f308", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 493, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000493__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-494-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 494, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "42937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 495, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "816", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "495", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d53ace52-f1ac-4e12-8256-84fc7cffe2d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 494, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000494__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-495-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 495, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 496, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "817", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "496", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bd0a047-cdd2-4ddd-94c6-b1b345c9b5c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 495, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000495__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-496-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 496, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 497, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "818", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "497", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59945917-d71d-4c14-95fd-c651e5f9b3f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 496, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000496__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-497-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 497, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 498, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "819", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "498", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da3197e0-2bac-439d-a423-c410f9192090", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 497, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000497__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-498-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 498, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 499, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "820", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "499", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f02ad1ba-c5e9-4c71-9836-ded43ac7a6e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 498, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000498__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-499-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 499, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 500, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "821", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "500", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18632bb9-a868-45cc-92d0-2b304e24e2dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 499, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000499__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-500-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 500, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 501, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "822", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "501", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c68a00a-ed34-4fbe-ae73-b17e7dcb18fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 500, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000500__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-501-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 501, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 502, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "823", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "502", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9d1b9f3-0bfb-46aa-9f07-6c8489175bef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 501, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000501__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-502-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 502, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 503, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "824", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "503", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f1b74de-3d61-48c5-9c57-e44f236a03e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 502, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000502__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-503-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 503, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 504, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "825", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "504", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b9c6202-3140-4557-8110-a518b2219f40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 43940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 503, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000503__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-504-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 504, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 505, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "826", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "505", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50385cb8-94fe-465e-b11d-4c37cfed97be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 504, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000504__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-505-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 505, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 506, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "827", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "506", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3103372c-678b-4df0-add3-47adf42da28f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 505, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000505__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-506-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 506, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "43984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 507, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "828", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "507", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14d43a87-78a8-4ffa-b2a2-e8cdb8612046", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 506, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000506__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-507-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 507, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 508, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "829", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "508", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7fa5bc4-663e-4334-86a8-b5ef46e553a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 507, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000507__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-508-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 508, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 509, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "830", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "509", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d3a9755-e131-424a-b845-65d21f66b511", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 508, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000508__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-509-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 509, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 510, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "831", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "510", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25bfc3f3-1572-4001-81d4-de9fadb2994d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 509, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000509__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-510-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 510, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 511, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "832", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "511", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98c1e6d1-b409-4200-a167-c62d46f1fae1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 510, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000510__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-511-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 511, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 512, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "833", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "512", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1d9a329-2d85-4f16-bbfd-3ee5fe437261", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 511, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000511__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-512-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 512, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 513, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "834", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "513", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3af4ebec-cc3d-475e-b2ef-2740938eaed5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 512, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000512__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-513-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 513, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 514, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "835", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "514", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8489dc1-d1cc-441e-b81a-1ff8b7503097", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 513, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000513__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-514-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 514, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 515, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "836", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "515", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "381e2bbe-70b1-4bfe-9f32-a3ae44a23d19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 514, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000514__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-515-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 515, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 516, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "837", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "516", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69a79ecc-6c61-4272-b931-d4005ee40ad0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 44986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 515, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000515__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-516-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 516, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 517, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "838", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "517", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65a0f937-09a1-4b3c-a650-24d5e0f976cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 516, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000516__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-517-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 517, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "44937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 518, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "839", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "518", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f9881be-aaa1-4829-a507-95a95c97b918", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 517, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000517__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-518-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 518, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 519, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "840", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "519", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b61d85d-f8d9-4211-a97d-1ea63202447e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 518, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000518__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-519-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 519, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 520, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "841", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "520", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8869c28-b20f-473a-8b0d-ab063ed4a3d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 519, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000519__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-520-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 520, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 521, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "842", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "521", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "761b8331-2aee-4654-8fa4-361f1f390bba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 520, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000520__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-521-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 521, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 522, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "843", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "522", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fcd899ba-34e6-4a49-9502-25b280e2bb01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 521, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000521__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-522-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 522, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 523, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "844", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "523", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1bb4213-dc95-4bdc-9716-015349a09ff6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 522, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000522__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-523-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 523, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 524, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "845", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "524", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae386acf-1197-421a-abc1-ddbf3b70f843", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 523, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000523__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-524-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 524, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 525, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "846", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "525", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b334137-c8f3-46cb-9456-5969ffef4ca3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 524, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000524__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-525-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 525, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 526, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "847", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "526", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c59a299-5313-4405-b7fc-66deb52a7937", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 525, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000525__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-526-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 526, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 527, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "848", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "527", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8442966-5433-45de-a480-dc505aaf2f82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 45940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 526, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000526__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-527-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 527, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 528, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "849", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "528", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "018cf227-34bf-4dd2-b3d9-0092f18965ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 527, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000527__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-528-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 528, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 529, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "850", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "529", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e734bc6b-4b9e-4ae9-83fa-9575e4a49f08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 528, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000528__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-529-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 529, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "45984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 530, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "851", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "530", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "113054eb-7980-4498-9fcb-a4e37f174045", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 529, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000529__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-530-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 530, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 531, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "852", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "531", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd2ba3f6-d224-4adc-acfb-fd97a7054d78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 530, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000530__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-531-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 531, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 532, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "853", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "532", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3140f683-b36a-4a85-8db6-9a0ce4e3bdc4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 531, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000531__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-532-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 532, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 533, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "854", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "533", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fee23a91-49a1-41df-8a26-1611466e8f48", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 532, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000532__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-533-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 533, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 534, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "855", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "534", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32b40a5e-c66f-4482-a562-1f7643a5e8c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 533, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000533__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-534-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 534, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 535, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "856", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "535", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2755de0-57ca-4b37-b640-fd58912291ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 534, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000534__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-535-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 535, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 536, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "857", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "536", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ee82b8d-2335-4954-b834-a392cfb35955", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 535, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000535__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-536-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 536, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 537, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "858", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "537", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64021fef-736c-4706-8260-bd3cea9e9fa8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 536, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000536__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-537-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 537, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 538, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "859", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "538", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59f3e8e2-6344-46bf-80b9-488dbc0c27dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 537, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000537__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-538-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 538, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 539, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "860", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "539", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7493a931-a0f8-4f3f-af90-0886de1b917a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 46986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 538, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000538__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-539-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 539, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 540, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "861", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "540", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67eb614d-9f55-49f2-90c4-bec9f9a4d828", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 539, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000539__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-540-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 540, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "46937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 541, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "862", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "541", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cba1a521-4c0a-4513-887f-b4ff18471878", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 540, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000540__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-541-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 541, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 542, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "863", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "542", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e824db5-a758-461a-8257-1f6fc9e1b3e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 541, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000541__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-542-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 542, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 543, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "864", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "543", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56ae3a0f-05b7-4422-b6d4-e21599b73ace", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 542, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000542__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-543-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 543, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 544, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "865", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "544", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "100ace20-5dfb-4fac-b683-c873c04b7312", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 543, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000543__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-544-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 544, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 545, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "866", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "545", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36e9d079-82c2-4058-8641-860c0be0d669", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 544, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000544__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-545-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 545, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 546, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "867", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "546", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2fa5ca82-523a-4ed1-922d-50c5582b8469", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 545, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000545__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-546-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 546, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 547, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "868", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "547", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7babb4e0-3610-4590-8048-d1ccd904f4e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 546, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000546__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-547-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 547, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 548, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "869", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "548", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c60ad065-d884-49c1-a55b-78a38efff3c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 547, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000547__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-548-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 548, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 549, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "870", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "549", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8887bf9-13eb-47e2-bc9f-229dbcce98d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 548, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000548__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-549-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 549, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 550, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "871", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "550", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d16b397f-7e11-4498-af55-9f921694f307", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 47940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 549, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000549__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-550-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 550, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 551, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "872", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "551", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88ab508c-443b-4b22-8eb6-e86c436be2c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 550, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000550__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-551-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 551, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 552, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "873", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "552", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c2000d9-7d4d-4d99-8ad4-96bb381d09c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 551, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000551__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-552-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 552, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "47968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 553, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "874", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "553", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6047baec-e2fc-4e5b-b98b-d8d0365fe85b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 552, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000552__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-553-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 553, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 554, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "875", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "554", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bac72808-c781-450b-8ece-64363802014b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 553, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000553__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-554-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 554, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 555, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "876", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "555", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02d1ba37-1fb2-4da4-a266-4376c1d9a6f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 554, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000554__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-555-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 555, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 556, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "877", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "556", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f097ba0e-fc70-4d71-88f3-02f7ddd31af3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 555, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000555__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-556-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 556, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 557, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "878", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "557", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df5fea62-77bc-4482-b39d-6ca81e82b6d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 556, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000556__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-557-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 557, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 558, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "879", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "558", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4fc4bca-04be-4ca4-b97a-644eaa54b613", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 557, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000557__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-558-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 558, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 559, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "880", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "559", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48940af7-ede3-4d1b-b4c5-5b1664edb7ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 558, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000558__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-559-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 559, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 560, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "881", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "560", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32589c63-85e0-42ed-9c8a-261a0fe38dbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 559, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000559__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-560-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 560, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 561, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "882", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "561", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9eb5ed8a-10ed-40f4-b558-a7215d140de2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 560, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000560__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-561-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 561, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 562, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "883", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "562", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd937a3c-884b-4eb5-9405-91cd717347bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 48971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 561, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000561__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-562-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 562, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 563, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "884", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "563", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d1dbacc-729e-4bf1-8143-51e5453f638e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 562, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000562__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-563-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 563, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "48921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 564, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "885", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "564", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "175930f5-c57e-4338-94b9-8182848b9360", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 563, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000563__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-564-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 564, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 565, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "886", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "565", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60c40ab1-4b1b-4614-b994-ccdafaa52b51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 564, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000564__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-565-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 565, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 566, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "887", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "566", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "928a246b-08ce-46d7-a456-59d034a22f98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 565, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000565__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-566-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 566, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 567, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "888", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "567", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "acad41b1-b931-4901-9b08-1753d7576214", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 566, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000566__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-567-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 567, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 568, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "889", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "568", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40037a81-d933-4c38-8ae7-8276c0f50d2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 567, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000567__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-568-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 568, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 569, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "890", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "569", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "798a0942-8f58-4ff0-9d5c-5ce44a1d9ad8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 568, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000568__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-569-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 569, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 570, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "891", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "570", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f12dc5c-67ea-4c4a-8d09-77923b0ff26b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 569, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000569__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-570-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 570, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 571, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "892", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "571", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e87020c6-278e-4f1d-8ee8-6587c2553c07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 570, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000570__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-571-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 571, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 572, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "893", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "572", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10a5bfbd-e634-4d06-88dd-d941844fc854", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 571, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000571__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-572-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 572, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 573, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "894", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "573", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46735c79-0e03-4aa1-a509-f943b027322f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 49924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 572, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000572__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-573-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 573, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 574, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "895", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "574", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "469375de-78c7-4ccf-a3ca-d41f93bc8cd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 573, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000573__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-574-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 574, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 575, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "896", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "575", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8168e4eb-d8f3-4f45-a24c-d4643a0ef4ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 574, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000574__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-575-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 575, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "49968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 576, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "897", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "576", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e91bf840-a6b1-47c5-a829-606a2dcd7b6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 575, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000575__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-576-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 576, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 577, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "898", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "577", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "027bea4e-d857-43eb-b02e-55a9cd51bd80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 576, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000576__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-577-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 577, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 578, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "899", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "578", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c17935e-561e-43f7-9ab7-30afa043a0a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 577, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000577__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-578-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 578, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 579, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "900", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "579", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32a814a7-7232-46fe-950a-f6432c389821", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 578, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000578__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-579-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 579, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 580, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "901", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "580", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39a52492-2816-48cc-aaa2-c4d089116d30", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 579, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000579__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-580-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 580, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 581, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "902", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "581", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f14b20f5-76fd-4988-bfa8-80aedad6d3c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 580, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000580__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-581-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 581, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 582, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "903", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "582", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cef5c423-0de3-4348-90a2-6c9e9700db5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 581, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000581__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-582-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 582, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 583, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "904", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "583", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "009bdbe7-6674-4f02-8181-f95948e19cca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 582, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000582__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-583-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 583, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 584, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "905", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "584", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa0454ee-c8df-4bf9-9191-c129a88d8ef9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 583, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000583__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-584-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 584, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 585, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "906", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "585", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a8dbb1b-c750-4f74-84c1-7195b0c25348", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 50971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 584, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000584__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-585-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 585, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 586, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "907", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "586", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0498ae9-3497-40f1-9799-ff8decd89461", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 585, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000585__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-586-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 586, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "50921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 587, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "908", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "587", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5982c836-07d8-4e2f-b9d6-f22cfabc3411", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 586, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000586__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-587-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 587, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 588, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "909", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "588", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b218b66-23ea-4c6d-9cde-6ce27dc63cee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 587, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000587__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-588-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 588, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 589, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "910", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "589", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9af1dcd6-0699-4012-b251-a36cb0ac79af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 588, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000588__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-589-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 589, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 590, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "911", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "590", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a22f403a-2738-4dcb-b2cf-1e893bf82ea0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 589, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000589__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-590-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 590, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 591, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "912", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "591", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dd234c2-a6b5-405d-90a7-17716936a137", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 590, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000590__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-591-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 591, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 592, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "913", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "592", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47bff848-8643-4d10-99fb-ebb16dc975fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 591, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000591__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-592-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 592, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 593, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "914", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "593", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0165265-63b7-406c-a9ef-e14064e5aecc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 592, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000592__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-593-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 593, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 594, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "915", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "594", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f14785f0-669c-4ba2-aa87-bb61199a1180", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 593, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000593__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-594-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 594, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 595, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "916", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "595", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e5c86a3-28bb-42fd-b5a9-0ff641a49d94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 594, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000594__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-595-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 595, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 596, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "917", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "596", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "316d987a-2f83-4d81-9f7e-a481321da1d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 51924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 595, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000595__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-596-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 596, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 597, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "918", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "597", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "803c19b7-424d-4144-a92f-24b7fe843fc2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 596, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000596__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-597-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 597, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 598, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "919", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "598", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07c7e964-62e4-430c-a45d-641665284c64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 597, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000597__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-598-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 598, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "51968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 599, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "920", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "599", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "042fe00a-4862-488e-bee8-071a420bc87e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 598, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000598__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-599-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 599, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 600, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "921", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "600", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f911518f-7d10-42df-89bf-94a6c6237ffa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 599, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000599__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-600-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 600, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 601, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "922", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "601", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a3cb28a-8784-4e4b-8b15-d42d1a98862e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 600, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000600__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-601-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 601, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 602, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "923", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "602", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb679b31-65d8-4af4-8f90-7bc450f963b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 601, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000601__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-602-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 602, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 603, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "924", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "603", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91319ca7-dd0e-4039-94fb-d1c9195953c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 602, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000602__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-603-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 603, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 604, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "925", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "604", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27001440-9300-4a13-b393-ec7a7907cffa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 603, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000603__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-604-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 604, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 605, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "926", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "605", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3a65e66-b611-4e8b-8298-af94cb995b99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 604, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000604__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-605-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 605, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 606, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "927", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "606", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2446ea6-e68b-4823-b6ad-10ba912a1606", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 605, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000605__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-606-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 606, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 607, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "928", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "607", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63e13469-f6ff-49e5-912a-524779dff03c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 606, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000606__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-607-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 607, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 608, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "929", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "608", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30fef3d3-02e7-40ef-9c76-86295baff579", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 52971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 607, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000607__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-608-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 608, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 609, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "930", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "609", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bff7a158-c351-41b8-8236-e23d57d137fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 608, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000608__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-609-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 609, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "52921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 610, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "931", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "610", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "270d47a4-4a75-4557-a96b-f5aa8e8302b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 609, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000609__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-610-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 610, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 611, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "932", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "611", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21ec60f8-f2a8-4795-9b11-0cf67f27a252", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 610, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000610__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-611-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 611, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 612, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "933", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "612", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4242618-228b-4f48-be55-fa41bf471d27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 611, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000611__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-612-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 612, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 613, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "934", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "613", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e41fcd6-39cb-4905-82e5-96dbc7b8250e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 612, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000612__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-613-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 613, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 614, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "935", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "614", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a95ebc73-b5c9-4e0c-936b-d79a66a66350", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 613, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000613__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-614-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 614, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 615, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "936", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "615", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26cceabd-4383-464c-9682-260a29bf705b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 614, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000614__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-615-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 615, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 616, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "937", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "616", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cdaa719-363e-44cd-8f7a-e82323fbac98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 615, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000615__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-616-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 616, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 617, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "938", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "617", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12a2fea6-9f07-41da-80af-85c7c1862005", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 616, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000616__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-617-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 617, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 618, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "939", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "618", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f886859-c9c8-453f-b69d-a9b5abe2d091", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 617, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000617__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-618-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 618, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 619, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "940", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "619", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d212e1b8-6a8e-482c-9159-816996a59e67", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 53924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 618, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000618__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-619-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 619, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 620, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "941", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "620", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba726b18-c150-45c5-b520-139656ec72e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 619, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000619__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-620-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 620, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 621, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "942", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "621", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8f07307-57a0-4f69-9659-a8c264df3402", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 620, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000620__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-621-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 621, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "53953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 622, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "943", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "622", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "430e753a-103e-4fcd-b1d1-69dc649d95e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 621, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000621__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-622-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 622, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 623, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "944", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "623", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17b83452-363c-417b-bd30-335b15e0423f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 622, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000622__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-623-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 623, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 624, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "945", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "624", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e24fa88-5be1-4f92-bdad-18730ab07e59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 623, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000623__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-624-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 624, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 625, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "946", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "625", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "947ac6e5-b8ea-472d-99d1-a9abe006af18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 624, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000624__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-625-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 625, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 626, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "947", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "626", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc39980d-a1c2-4c0f-92cd-c42153938f8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 625, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000625__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-626-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 626, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 627, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "948", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "627", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55b3275f-21ef-4af4-8703-8e21c032bfb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 626, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000626__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-627-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 627, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 628, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "949", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "628", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a568cfd-30cf-4d60-87f3-05a8feebb9a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 627, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000627__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-628-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 628, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 629, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "950", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "629", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3dd1cc6c-73dc-4931-8b34-b97d89dd2007", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 628, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000628__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-629-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 629, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 630, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "951", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "630", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e89a659-bec3-4bf2-9ae5-ca4b950ad919", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 629, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000629__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-630-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 630, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 631, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "952", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "631", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee2102d4-22bf-4169-9bd3-6ed9b4852671", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 54955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 630, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000630__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-631-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 631, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 632, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "953", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "632", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a533ecc6-527e-4b62-a90e-9db41c7dce3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 631, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000631__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-632-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 632, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "54906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 633, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "954", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "633", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92bc00bb-3ce5-442e-be32-4f9ca1a7e22a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 632, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000632__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-633-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 633, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 634, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "955", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "634", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "973b02eb-001b-4aaa-94af-78feb7b6f262", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 633, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000633__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-634-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 634, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 635, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "956", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "635", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4839234c-c5af-443c-afc4-0241e97a5281", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 634, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000634__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-635-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 635, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 636, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "957", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "636", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52013f08-99ef-454e-8855-c002b9be2e60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 635, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000635__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-636-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 636, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 637, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "958", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "637", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f077fdc-5c49-4ccd-a6ea-a2205c45ec9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 636, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000636__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-637-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 637, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 638, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "959", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "638", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db14d8da-36c3-4dcd-9868-461550006dd4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 637, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000637__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-638-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 638, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 639, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "960", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "639", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca91bcdb-d3fb-4790-9ff6-ffccca9b2b09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 638, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000638__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-639-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 639, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 640, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "961", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "640", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bd722ae-3374-40b7-85e0-2a86a0419617", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 639, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000639__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-640-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 640, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 641, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "962", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "641", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9ddfc43-c21e-438a-81ae-35f1323ab032", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 640, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000640__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-641-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 641, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 642, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "963", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "642", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1cdb219f-7b90-499d-afee-f39a86036799", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 55908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 641, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000641__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-642-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 642, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 643, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "964", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "643", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baa40d78-0905-4423-9565-2403af3857e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 642, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000642__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-643-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 643, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 644, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "965", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "644", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cadc4153-7653-43b2-876d-d443bae88321", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 643, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000643__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-644-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 644, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "55953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 645, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "966", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "645", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6920e53e-9f18-493e-8a3b-e43be48b4bc3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 644, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000644__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-645-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 645, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 646, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "967", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "646", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79865acb-aad2-42da-a628-dbfe64348a4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 645, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000645__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-646-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 646, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 647, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "968", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "647", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "129a136b-420b-4c98-aadf-53f04c15690f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 646, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000646__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-647-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 647, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 648, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "969", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "648", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6646f303-65f7-40cf-81de-1551f7c02d18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 647, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000647__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-648-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 648, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 649, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "970", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "649", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f14d136-0c61-4dfe-bd92-272d12e9f193", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 648, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000648__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-649-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 649, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 650, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "971", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "650", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4bff994-8b87-4e86-92f4-1695f16228fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 649, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000649__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-650-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 650, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 651, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "972", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "651", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6e196b2-a3c9-41a5-8117-b5dbf2e99b12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 650, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000650__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-651-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 651, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 652, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "973", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "652", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e37b244-6836-4c96-9688-dd795bd499a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 651, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000651__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-652-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 652, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 653, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "974", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "653", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b7ba1d66-4ab1-4377-b2e8-6654e5c48478", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 652, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000652__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-653-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 653, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 654, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "975", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "654", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fcf12ec-1db3-41a7-8403-e2da1719e2e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 56955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 653, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000653__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-654-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 654, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 655, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "976", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "655", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9436b41d-abc1-4f28-82e5-0045d6d44a16", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 654, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000654__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-655-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 655, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "56906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 656, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "977", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "656", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47e1e053-1b04-4237-ac2c-f19ba56ef3c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 655, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000655__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-656-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 656, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 657, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "978", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "657", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20b59b74-9ff6-4adc-bcd6-8536190c6a6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 656, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000656__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-657-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 657, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 658, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "979", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "658", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "49fd3e89-37b9-441d-846f-05f24e5c2489", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 657, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000657__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-658-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 658, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 659, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "980", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "659", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2197d78-ce6a-4402-9fe5-e159527fcb18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 658, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000658__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-659-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 659, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 660, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "981", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "660", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e048a971-17ce-43a5-863b-b89fc733a697", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 659, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000659__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-660-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 660, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 661, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "982", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "661", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a68b118-3560-494c-a4fe-f83b09490b59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 660, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000660__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-661-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 661, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 662, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "983", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "662", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "449706c9-3e37-4bad-8f61-0623b3467205", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 661, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000661__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-662-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 662, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 663, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "984", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "663", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15dd8b1d-f10f-4c98-b050-fe678380ee4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 662, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000662__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-663-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 663, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 664, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "985", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "664", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7273c4ec-fd8d-4d7d-a613-75b70ca6d7b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 663, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000663__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-664-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 664, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 665, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "986", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "665", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eea62211-1fef-4a21-9c9b-65ef99a4b5fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 57908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 664, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000664__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-665-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 665, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 666, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "987", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "666", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ad8efe1-d384-42d2-9d45-aad78a1f56af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 665, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000665__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-666-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 666, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 667, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "988", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "667", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b8d84eb-141c-4795-8f4f-d85acedf7598", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 666, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000666__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-667-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 667, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "57953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 668, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "989", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "668", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb6c3666-77ed-400e-9f3c-e4df75b10d60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 667, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000667__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-668-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 668, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 669, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "990", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "669", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10e309bb-6214-421d-bfb3-dc0146bdeb4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 668, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000668__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-669-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 669, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 670, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "991", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "670", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "733a820c-63c9-4e01-be12-7490a39b4875", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 669, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000669__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-670-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 670, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 671, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "992", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "671", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd8c6a9b-8f72-48a3-9490-6928aba5f5b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 670, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000670__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-671-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 671, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 672, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "993", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "672", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccd7953c-ee2e-45cd-b32b-0c036b2f220b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 671, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000671__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-672-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 672, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 673, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "994", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "673", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b078e406-7e07-4bb2-9d1e-687420f2051b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 672, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000672__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-673-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 673, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 674, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "995", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "674", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcdf8faf-3555-49c5-894a-76bf53835644", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 673, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000673__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-674-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 674, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 675, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "996", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "675", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca5b9b4c-af45-49fe-ad5d-f43f0a3ccaa1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 674, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000674__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-675-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 675, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 676, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "997", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "676", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5ba5842-623d-4fa5-ad36-6cb261be893d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 675, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000675__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-676-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 676, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 677, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "998", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "677", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90da748b-9ada-496a-805f-c9fef94bab28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 58955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 676, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000676__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-677-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 677, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 678, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "999", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "678", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48c5be85-a96b-4225-bc4b-96b45f92f583", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 677, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000677__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-678-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 678, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 679, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1000", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "679", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "711eee85-c6a3-44bd-b6c6-85756f54113d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 678, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000678__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-679-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 679, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "58984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 680, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1001", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "680", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc273648-3c58-4ff3-9861-19daaf733824", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 679, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000679__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-680-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 680, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 681, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1002", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "681", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30afe146-89cf-4d8f-96e9-4bb63dfb07ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 680, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000680__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-681-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 681, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 682, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1003", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "682", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57638169-8e23-4ff1-ba73-a1c10b9cc74e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 681, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000681__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-682-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 682, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 683, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1004", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "683", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9b084f4-dd1c-437a-b2de-265c2a8dd3fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 682, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000682__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-683-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 683, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 684, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1005", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "684", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1930aba1-cccc-4755-b789-f5465c7fa546", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 683, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000683__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-684-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 684, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 685, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1006", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "685", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b95d656b-8e89-47c1-ba10-98957c2a28fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 684, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000684__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-685-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 685, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 686, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1007", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "686", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60e7fa7e-3c2c-4a32-bdbd-2a2bdf93c664", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 685, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000685__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-686-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 686, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 687, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1008", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "687", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "405a541b-3767-4c1e-8bc5-b6c3a39baca2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 686, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000686__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-687-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 687, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 688, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1009", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "688", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cecd474-0f07-4e5b-9f47-a2a2522d04ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 687, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000687__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-688-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 688, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 689, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1010", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "689", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0408ca7-295a-49ad-8801-d51cbdba8634", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 59986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 688, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000688__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-689-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 689, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 690, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1011", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "690", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "632d3325-8862-4e40-9b99-e10c0732ec82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 689, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000689__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-690-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 690, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "59937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 691, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1012", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "691", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6cfde54-9ab9-4e20-9fe1-80188848fbdb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 690, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000690__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-691-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 691, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 692, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1013", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "692", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "170e005a-e561-4812-b068-8f00e08ab011", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 691, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000691__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-692-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 692, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 693, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1014", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "693", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4549da66-f62d-4152-afe5-d90cb76a7438", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 692, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000692__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-693-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 693, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 694, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1015", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "694", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0707aef3-0586-4080-8de6-03e18dd29070", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 693, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000693__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-694-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 694, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 695, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1016", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "695", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d659fe25-86ae-4ec1-9679-7be2645f1a9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 694, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000694__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-695-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 695, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 696, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1017", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "696", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f978e0e-0aba-470b-9c51-a0373faaf913", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 695, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000695__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-696-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 696, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 697, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1018", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "697", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05f0676c-59b4-44df-a947-2ad336d78c0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 696, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000696__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-697-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 697, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 698, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1019", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "698", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e01c0ed-66c3-423d-b74d-5fb2b537f390", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 697, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000697__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-698-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 698, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 699, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1020", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "699", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20b4bce6-3f43-4381-bbaa-1fc945a84c4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 698, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000698__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-699-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 699, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 700, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1021", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "700", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "303ebd1d-222b-4203-8402-859c3e03128e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 60940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 699, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000699__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-700-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 700, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 701, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1022", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "701", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1da90d6b-c2db-4aae-ba04-c5539fe598ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 700, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000700__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-701-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 701, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 702, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1023", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "702", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d388113d-2563-45fb-8aa5-1766353de43c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 701, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000701__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-702-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 702, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "60984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 703, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1024", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "703", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e214aff4-5c79-485f-bceb-cdf2104f145c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 702, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000702__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-703-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 703, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 704, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1025", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "704", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07b2a9aa-ca29-4767-a5ca-04ffd96a0cce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 703, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000703__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-704-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 704, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 705, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1026", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "705", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e11ccfdf-c90f-4689-a9da-895ce5d747d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 704, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000704__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-705-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 705, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 706, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1027", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "706", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb88db4c-0221-4591-bc02-7ec2e192c4ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 705, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000705__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-706-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 706, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 707, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1028", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "707", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef7115d3-3da3-4a44-9a48-5fb906080631", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 706, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000706__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-707-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 707, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 708, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1029", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "708", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87740b75-42ee-4405-90c7-066d0f7cc374", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 707, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000707__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-708-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 708, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 709, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1030", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "709", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f75793af-2da8-4e79-addf-9c43efe3b8a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 708, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000708__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-709-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 709, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 710, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1031", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "710", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f2aa6cb-c2e2-4dc1-8885-d6975246f361", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 709, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000709__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-710-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 710, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 711, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1032", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "711", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b9d7028-dcd9-484b-8d04-bb5d3c4581a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 710, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000710__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-711-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 711, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 712, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1033", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "712", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2c6c8ad-68d7-49f8-aa0d-981fc7989ccc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 61986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 711, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000711__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-712-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 712, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 713, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1034", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "713", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6c55a00-c6b5-430f-aca9-a5859abd49ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 712, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000712__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-713-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 713, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "61937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 714, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1035", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "714", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c276b7f-4b02-4145-bd23-d9c30bf77179", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 713, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000713__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-714-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 714, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 715, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1036", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "715", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0d1103f-5289-4eef-b0c0-842f12a7a80d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 714, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000714__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-715-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 715, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 716, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1037", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "716", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0105ec7a-03d3-44b2-8ced-710cbfea5e01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 715, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000715__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-716-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 716, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 717, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1038", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "717", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ef33079-ecab-42ab-9d6b-b3997e1ddf62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 716, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000716__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-717-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 717, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 718, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1039", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "718", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e64316a-26a4-40e9-90b7-fc692e586a24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 717, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000717__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-718-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 718, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 719, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1040", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "719", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c2a8112-bcc4-44a8-bcd2-e23d9ca447dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 718, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000718__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-719-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 719, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 720, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1041", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "720", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "352603e8-9ecd-4f63-bfd3-79d71267d7d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 719, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000719__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-720-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 720, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 721, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1042", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "721", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62bbc5de-bb35-47a0-bf82-94b95dc76cd6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 720, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000720__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-721-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 721, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 722, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1043", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "722", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32e0c124-dbf0-4470-a1e9-00e653b792cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 721, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000721__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-722-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 722, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 723, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1044", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "723", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4760fe52-7b92-48ed-ae0c-37b1a7820a70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 62940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 722, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000722__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-723-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 723, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 724, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1045", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "724", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "993b759a-a617-44d1-a51c-75dc6d1d15d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 723, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000723__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-724-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 724, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 725, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1046", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "725", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baccafe0-9470-4d3b-9de1-53e7de2ea935", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 724, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000724__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-725-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 725, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "62984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 726, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1047", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "726", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "176e3cee-11d3-4d72-a778-5855d8468645", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 725, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000725__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-726-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 726, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 727, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1048", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "727", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aecc23c7-dc14-446f-877e-91ddc2b825f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 726, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000726__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-727-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 727, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 728, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1049", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "728", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c32b6f49-894a-41e3-99a5-b12d813c697c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 727, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000727__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-728-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 728, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 729, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1050", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "729", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "996cae97-0455-44c2-ad94-33f047ffe9f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 728, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000728__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-729-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 729, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 730, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1051", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "730", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63e11569-d300-4208-af72-cddce23f9dda", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 729, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000729__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-730-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 730, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 731, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1052", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "731", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66899225-d9f1-4c9f-940e-87e672c04037", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 730, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000730__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-731-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 731, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 732, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1053", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "732", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e54b70d8-0301-4992-b66f-ffd6a38d1d49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 731, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000731__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-732-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 732, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 733, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1054", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "733", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "858018aa-a845-44fd-b25d-460efec1b792", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 732, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000732__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-733-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 733, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 734, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1055", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "734", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3b7051a-0e2c-47c2-844d-2bc7290bbbb2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 733, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000733__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-734-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 734, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 735, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1056", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "735", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b72c018-8eae-4fe8-8089-61124fad6cb1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 63986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 734, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000734__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-735-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 735, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 736, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1057", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "736", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6937afd-10c9-4c14-9d06-2f608453232e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 735, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000735__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-736-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 736, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "63937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 737, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1058", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "737", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8b9b205-4107-433d-986c-f9a12ceb319d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 736, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000736__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-737-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 737, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 738, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1059", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "738", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9ac2160-e7e1-4689-88cc-b24424f675e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 737, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000737__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-738-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 738, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 739, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1060", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "739", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7497671b-26c1-4ef7-bcd7-14a767d924d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 738, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000738__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-739-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 739, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 740, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1061", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "740", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d8c06db-8ecc-436e-8f6d-53013d9b9f1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 739, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000739__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-740-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 740, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 741, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1062", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "741", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1234ef9b-3d12-42ff-a725-5fff04b2e251", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 740, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000740__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-741-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 741, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 742, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1063", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "742", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6515ca39-cb40-454c-8bdf-36ccafafe2d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 741, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000741__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-742-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 742, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 743, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1064", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "743", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a85e635-15f9-46dc-ac0e-7672f3fc44c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 742, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000742__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-743-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 743, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 744, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1065", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "744", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1338d11-47e7-42fb-9156-80405d1dc31b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 743, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000743__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-744-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 744, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 745, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1066", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "745", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bab5727b-3818-4573-9149-c9f369233d15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 744, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000744__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-745-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 745, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 746, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1067", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "746", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94f4c4bd-ab7b-4560-9b70-d45778e48bcc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 64940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 745, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000745__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-746-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 746, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 747, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1068", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "747", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "daad2702-7dd7-4d66-846a-80c87ed94c08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 746, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000746__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-747-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 747, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 748, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1069", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "748", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f5accc7-5ce7-47b7-9d8e-39de8b6d7c6f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 747, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000747__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-748-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 748, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "64968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 749, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1070", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "749", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f91bda11-8f00-4ad7-8a04-99341bd8340e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 748, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000748__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-749-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 749, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 750, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1071", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "750", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a108bcc-f3a5-4bf2-8a46-ec6cee17f036", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 749, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000749__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-750-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 750, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 751, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1072", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "751", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53c42e7b-990f-411c-9373-0f7282c13f09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 750, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000750__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-751-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 751, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 752, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1073", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "752", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d197c130-3291-42f9-ae37-fc97bd08deaf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 751, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000751__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-752-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 752, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 753, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1074", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "753", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1a5091c-f6bc-4744-8617-156556cd8a1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 752, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000752__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-753-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 753, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 754, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1075", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "754", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc34c162-11e4-425f-a553-b60b4be696bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 753, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000753__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-754-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 754, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 755, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1076", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "755", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8137359-b038-45a3-98e4-705038e8c3e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 754, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000754__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-755-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 755, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 756, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1077", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "756", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22ad2dea-85ce-4017-b33e-4a3c5b197a08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 755, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000755__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-756-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 756, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 757, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1078", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "757", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fe5d2e7-b43b-4896-bbdc-55806311ad6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 756, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000756__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-757-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 757, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 758, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1079", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "758", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b966ce6b-6a81-4dc3-bb4b-b2c8181198de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 65971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 757, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000757__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-758-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 758, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 759, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1080", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "759", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ada0c31d-398c-43a0-bde5-74f53605f6f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 758, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000758__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-759-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 759, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "65921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 760, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1081", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "760", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0e4405c-3fff-4daa-890c-81617b1631e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 759, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000759__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-760-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 760, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 761, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1082", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "761", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a4b318d-1ddf-41c1-9732-695cec1af157", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 760, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000760__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-761-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 761, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 762, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1083", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "762", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de879574-b43d-46d9-b4d7-101a11395233", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 761, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000761__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-762-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 762, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 763, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1084", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "763", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8dec885-3c2e-4d0a-b6b4-027e2e872dea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 762, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000762__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-763-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 763, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 764, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1085", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "764", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75f30d2f-472a-4938-adac-8329aa62a72b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 763, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000763__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-764-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 764, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 765, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1086", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "765", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11eb81de-1c7f-4c56-8361-fe19e5dbaad9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 764, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000764__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-765-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 765, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 766, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1087", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "766", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1f7b745-3568-4a3c-8f73-f7f9fbe592cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 765, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000765__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-766-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 766, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 767, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1088", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "767", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d824eb50-d2b8-4062-aff5-c9b7ceef580f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 766, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000766__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-767-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 767, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 768, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1089", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "768", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "649f700d-08c1-4f1c-a30a-cff213a95beb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 767, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000767__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-768-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 768, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 769, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1090", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "769", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12791e91-1297-4b10-8f5f-c01b2cf59184", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 66924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 768, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000768__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-769-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 769, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 770, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1091", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "770", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3632209d-c0d1-4a0d-ac73-d52ae4d998f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 769, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000769__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-770-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 770, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 771, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1092", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "771", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd77055f-ff32-4cf7-a4ae-963f04ebe7a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 770, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000770__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-771-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 771, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "66968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 772, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1093", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "772", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "647bc18b-1c0f-4049-9ff1-9c650975657b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 771, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000771__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-772-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 772, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 773, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1094", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "773", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7971fa40-d0e5-405b-95ed-037ff24ab7fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 772, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000772__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-773-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 773, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 774, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1095", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "774", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b07414e-39ba-4fa5-8bcb-b29ca130f582", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 773, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000773__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-774-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 774, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 775, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1096", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "775", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ad4ab7e-bcc9-4781-af9a-ee295eedba28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 774, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000774__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-775-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 775, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 776, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1097", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "776", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97176283-a8d7-40af-8842-ea547f61dc8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 775, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000775__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-776-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 776, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 777, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1098", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "777", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d365e600-67fb-401c-95ce-a851866ef393", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 776, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000776__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-777-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 777, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 778, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1099", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "778", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ceb17605-771f-4374-b2ac-b072fcbb3b67", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 777, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000777__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-778-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 778, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 779, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1100", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "779", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad0ab2f7-5e1c-4294-97b5-acd60ce7e76a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 778, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000778__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-779-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 779, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 780, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1101", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "780", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88ba5bf4-f969-4c9f-86bc-02bc45cc5ed2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 779, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000779__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-780-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 780, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 781, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1102", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "781", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a59d5f5-5d6a-4229-8a66-179fac5dcd7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 67971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 780, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000780__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-781-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 781, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 782, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1103", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "782", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "841e3818-71e7-42e0-82b1-d0eeae8dfc7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 781, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000781__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-782-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 782, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "67921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 783, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1104", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "783", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85455067-93f3-45aa-a8cb-e8f091637764", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 782, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000782__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-783-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 783, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 784, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1105", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "784", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5376e075-0aa5-453a-abbf-781ea9a12891", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 783, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000783__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-784-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 784, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 785, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1106", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "785", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee4bd0d4-4d06-44aa-9562-c4153e80b931", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 784, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000784__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-785-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 785, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 786, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1107", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "786", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f52215c-68cd-4763-990a-0f937705ca0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 785, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000785__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-786-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 786, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 787, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1108", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "787", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7921617a-7c12-4087-9b38-77fc6329a70e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 786, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000786__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-787-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 787, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 788, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1109", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "788", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "850c25f0-356d-40af-b11d-7b9e971a2ed6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 787, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000787__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-788-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 788, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 789, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1110", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "789", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd5a4c51-a8b0-46c4-8d55-641371b21b7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 788, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000788__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-789-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 789, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 790, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1111", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "790", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "769b440f-69d6-4e7a-a6d7-43827ab490a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 789, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000789__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-790-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 790, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 791, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1112", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "791", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "594073af-0b3a-4d25-a3c3-4ed8e5a567fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 790, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000790__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-791-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 791, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 792, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1113", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "792", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "248690aa-5cb2-48c9-8cea-95b78d58876b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 68924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 791, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000791__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-792-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 792, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 793, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1114", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "793", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a03721cf-738c-4f58-9182-d6fb133fd8f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 792, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000792__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-793-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 793, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 794, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1115", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "794", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ad02556-b15e-4a96-9736-c6eaed41da07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 793, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000793__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-794-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 794, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "68968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 795, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1116", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "795", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "946cde9a-f789-420e-bccc-f21e7be6064d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 794, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000794__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-795-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 795, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 796, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1117", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "796", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64f5a50a-fbdb-48a8-a2d4-c628173daa1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 795, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000795__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-796-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 796, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 797, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1118", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "797", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "194acebb-5842-4411-960e-c98741c4fc6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 796, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000796__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-797-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 797, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 798, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1119", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "798", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a68441e-7626-4a48-ad09-11e122f41514", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 797, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000797__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-798-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 798, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 799, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1120", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "799", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9bd63bf-c33b-4e4c-8e50-26542ce4c5a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 798, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000798__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-799-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 799, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 800, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1121", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "800", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a06b5c2-531c-47d6-9bba-ab97461021c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 799, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000799__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-800-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 800, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 801, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1122", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "801", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf8a1678-a33d-4b73-8d4e-8eb7e9d72787", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 800, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000800__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-801-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 801, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 802, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1123", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "802", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4215bbff-b07a-4e04-ac43-011ab080c307", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 801, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000801__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-802-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 802, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 803, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1124", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "803", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d51108b-4480-4d57-be5b-8439820dac5a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 802, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000802__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-803-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 803, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 804, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1125", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "804", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e7a2f0e-0471-46ef-b590-410fbf8e8050", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 69971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 803, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000803__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-804-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 804, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 805, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1126", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "805", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6645825a-53f5-455b-8017-5c59276d4df7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 804, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000804__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-805-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 805, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "69921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 806, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1127", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "806", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1929d561-795e-4629-819b-f21013b1d4e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 805, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000805__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-806-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 806, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 807, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1128", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "807", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f687ea42-2d13-42df-8084-d9a10ef943e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 806, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000806__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-807-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 807, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 808, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1129", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "808", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b845f6ec-9371-4e25-98df-2069b842f0b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 807, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000807__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-808-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 808, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 809, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1130", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "809", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5a2b96c-800f-4380-b675-4672c1d9ac9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 808, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000808__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-809-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 809, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 810, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1131", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "810", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f4a1066-6e3b-4547-b0b3-3f979540cc9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 809, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000809__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-810-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 810, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 811, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1132", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "811", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52e13007-7307-450a-a545-7032440160fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 810, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000810__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-811-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 811, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 812, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1133", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "812", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d53e927f-3e8f-4386-bf6d-17e4f76f4d10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 811, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000811__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-812-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 812, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 813, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1134", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "813", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7e6472b-c4c5-4957-b0f7-b35c5f6d9d6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 812, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000812__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-813-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 813, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 814, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1135", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "814", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "785eb95f-9d02-4c1e-aee1-f9ff587382b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 813, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000813__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-814-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 814, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 815, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1136", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "815", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8675f9e-1ebd-486c-b585-afe73a94322c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 70924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 814, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000814__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-815-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 815, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 816, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1137", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "816", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0b02ad8-8faf-424f-87c1-6d9cb48f2e9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 815, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000815__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-816-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 816, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 817, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1138", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "817", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f5a4a1d-cf8c-4607-a14c-a9ffc01cd31b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 816, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000816__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-817-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 817, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "70953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 818, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1139", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "818", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0114db8-2c75-4318-9120-a578a3e9f100", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 817, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000817__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-818-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 818, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 819, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1140", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "819", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63d43b80-bdac-4afc-a6f7-14c7f06d2d15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 818, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000818__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-819-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 819, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 820, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1141", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "820", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "233d2f28-99d8-4c9d-8339-79603ee24c11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 819, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000819__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-820-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 820, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 821, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1142", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "821", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "beefec5f-8bbf-44c6-9c91-34ec0eb76b15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 820, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000820__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-821-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 821, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 822, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1143", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "822", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7aca2b1f-5a1f-496f-b79a-45dbb368d58b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 821, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000821__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-822-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 822, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 823, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1144", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "823", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2422f909-a7e4-4612-b85d-a3f8d3d4b5ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 822, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000822__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-823-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 823, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 824, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1145", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "824", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "723060d4-9854-4dd5-8dc8-a91cc1253c9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 823, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000823__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-824-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 824, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 825, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1146", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "825", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b5719c3-b6e4-4392-9a22-2901ae9be1d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 824, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000824__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-825-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 825, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 826, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1147", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "826", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db10f78c-1c6a-499c-98d8-b4d179fdc359", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 825, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000825__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-826-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 826, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 827, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1148", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "827", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e82b96e-55dc-487d-bc60-490ecb9cdc3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 71955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 826, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000826__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-827-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 827, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 828, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1149", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "828", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "155a6ff7-db93-433f-8acd-db852d9d0fdd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 827, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000827__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-828-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 828, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "71906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 829, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1150", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "829", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e3074c3-c8dc-4255-aec1-78423917ce2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 828, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000828__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-829-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 829, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 830, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1151", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "830", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c1b30ba-1072-473a-9d02-780432bc2b79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 829, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000829__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-830-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 830, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 831, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1152", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "831", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5aad8d1-b4b1-49c8-ab64-13926f57cfc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 830, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000830__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-831-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 831, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 832, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1153", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "832", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1ca130f-a516-4a14-a89f-ffb7e508f4ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 831, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000831__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-832-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 832, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 833, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1154", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "833", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d9865a3-76a0-4de4-8d22-1b119d844840", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 832, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000832__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-833-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 833, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 834, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1155", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "834", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e866a51a-e1a3-42c6-a01c-9c092657347d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 833, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000833__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-834-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 834, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 835, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1156", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "835", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f40c3eb-aa2d-4770-90a6-3b23c1b2bb34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 834, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000834__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-835-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 835, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 836, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1157", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "836", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0c9ff84-5ce8-4328-aba2-42eeec573c8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 835, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000835__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-836-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 836, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 837, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1158", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "837", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f82cbdc4-e5a6-40b3-be80-a4d8d5a12ed2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 836, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000836__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-837-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 837, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 838, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1159", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "838", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "235fdc62-48b6-417d-a9fd-0c067270d179", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 72908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 837, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000837__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-838-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 838, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 839, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1160", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "839", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa235fa0-bad5-47be-8363-6dfaf66947c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 838, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000838__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-839-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 839, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 840, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1161", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "840", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f47f465-8872-4100-b8eb-fb0b0a493bad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 839, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000839__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-840-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 840, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "72953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 841, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1162", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "841", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c00671b-42cf-439c-9aa3-65d40dbd0da4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 840, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000840__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-841-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 841, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 842, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1163", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "842", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aeed34cc-6e43-4c4b-b3c8-dd43f1a16167", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 841, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000841__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-842-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 842, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 843, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1164", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "843", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da06246d-bc95-4c54-ac34-a27220d24a66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 842, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000842__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-843-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 843, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 844, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1165", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "844", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24d1b107-1b81-4d30-b768-519aa856817b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 843, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000843__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-844-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 844, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 845, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1166", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "845", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e4a8382-2240-4c95-ab5e-67b22ea487d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 844, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000844__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-845-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 845, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 846, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1167", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "846", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1a18d26-de3f-4917-b1a7-82cd5649d79c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 845, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000845__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-846-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 846, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 847, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1168", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "847", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b05200d-225c-4230-a764-1939c58596c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 846, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000846__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-847-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 847, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 848, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1169", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "848", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f14ca8da-91ef-485f-bce1-2a6e421e066e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 847, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000847__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-848-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 848, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 849, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1170", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "849", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bdc2bc7-848a-4aba-aecd-c21c6afd82e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 848, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000848__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-849-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 849, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 850, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1171", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "850", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07c299fd-5b9a-4e9c-9503-7171dcc82415", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 73955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 849, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000849__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-850-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 850, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 851, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1172", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "851", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b67d2072-9b74-4b98-90ee-ec005501f917", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 850, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000850__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-851-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 851, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "73906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 852, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1173", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "852", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13f3b12c-d3a5-476c-b87d-835e5b2ea9c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 851, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000851__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-852-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 852, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 853, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1174", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "853", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c32d9d40-538e-4f61-b597-277daa5b0ec0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 852, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000852__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-853-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 853, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 854, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:19:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1175", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "854", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b387d34-7e40-4f74-a395-8a7a19ef5f7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 853, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000853__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-854-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 854, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 855, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1176", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "855", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55928c6a-c901-48a6-836f-11e98139383c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 854, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000854__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-855-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 855, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 856, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1177", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "856", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ceacbeed-d492-41d8-88f6-887a8b0a5c72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 855, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000855__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-856-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 856, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 857, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1178", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "857", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "323c5df4-bf9e-4482-b9be-9b3fd8508d24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 856, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000856__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-857-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 857, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 858, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1179", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "858", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e7130cbf-bf0e-44cf-b26d-0bdd846f336e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 857, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000857__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-858-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 858, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 859, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1180", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "859", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15c677c1-ed84-4f62-9287-e90cff602f15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 858, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000858__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-859-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 859, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 860, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1181", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "860", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "901536cc-84f7-4b67-9572-882d32de322e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 859, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000859__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-860-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 860, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 861, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1182", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "861", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8eeebc69-33f0-4fe5-b9ea-a382128b1102", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 74908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 860, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000860__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-861-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 861, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 862, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1183", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "862", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51b1df28-c334-4766-8f5a-15d738dccf98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 861, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000861__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-862-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 862, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 863, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1184", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "863", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0201c2b7-0c2e-4c7b-b388-80fadd3f9658", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 862, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000862__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-863-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 863, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "74953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 864, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1185", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "864", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aeaa3c9b-bcc4-40e6-ab3d-2aac5d04ebcb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 863, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000863__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-864-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 864, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 865, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1186", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "865", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26ced139-2b20-40e1-8309-17fc224926a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 864, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000864__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-865-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 865, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 866, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1187", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "866", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ad2aa14-670e-4d1e-9c7a-c016abdb1c46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 865, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000865__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-866-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 866, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 867, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1188", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "867", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "975da91d-d0d1-4f9f-aa92-1a77ab7e8f7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 866, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000866__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-867-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 867, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 868, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1189", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "868", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75bcb20e-f162-406a-8957-e8b2d1d5b056", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 867, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000867__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-868-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 868, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 869, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1190", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "869", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c91c4d7a-0c46-4ee2-b0cd-ade59cec939c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 868, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000868__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-869-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 869, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 870, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1191", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "870", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebd989d7-fc8c-4c95-9997-45b702ef2d6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 869, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000869__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-870-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 870, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 871, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1192", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "871", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8290ddb-e44d-4739-a752-aa886e2c3857", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 870, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000870__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-871-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 871, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 872, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1193", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "872", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8da16bb3-1b0c-4df5-ba00-8dfa15b75dcc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 871, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000871__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-872-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 872, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 873, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1194", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "873", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbc3df96-09f1-41d5-87a5-749e3360a3da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 75955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 872, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000872__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-873-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 873, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 874, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1195", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "874", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd5aa859-07c3-4752-aeaa-f22facf0e562", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 873, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000873__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-874-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 874, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 875, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1196", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "875", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "432c0cfc-f3d0-4efe-ada6-0772c643f464", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 874, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000874__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-875-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 875, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "75984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 876, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1197", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "876", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4f4fc07-ac01-4046-a11c-fe20de21a733", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 875, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000875__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-876-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 876, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 877, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1198", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "877", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f1d67dd-f1aa-4c00-b57f-69098454bfad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 876, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000876__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-877-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 877, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 878, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1199", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "878", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b822f98-7293-48ab-b89f-52cfae545ee0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 877, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000877__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-878-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 878, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 879, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1200", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "879", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9fdefe4-5529-4f84-a250-95af33d55a98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 878, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000878__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-879-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 879, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 880, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1201", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "880", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bae88106-c494-4885-965c-7d865a5eafbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 879, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000879__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-880-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 880, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 881, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1202", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "881", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36cb1aaa-48ae-4095-8ba7-2a27ede2469b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 880, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000880__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-881-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 881, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 882, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1203", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "882", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "465ead6a-578c-4128-9e37-0eacbc84d5a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 881, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000881__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-882-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 882, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 883, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1204", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "883", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "176b9c54-870e-4407-b128-53a93b75d502", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 882, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000882__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-883-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 883, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 884, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1205", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "884", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d191287-dd19-4c40-8197-c60f1ceffed0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 883, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000883__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-884-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 884, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 885, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1206", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "885", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "494c0c1a-4e85-47ca-91c4-9e7aac3a72b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 76986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 884, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000884__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-885-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 885, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 886, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1207", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "886", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b01e2f1-1d14-4ca4-abe6-e012d2667aa3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 885, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000885__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-886-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 886, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "76937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 887, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1208", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "887", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "892ebefb-9467-44e0-a7e9-e0dea74e7e27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 886, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000886__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-887-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 887, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 888, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1209", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "888", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2117b821-daf8-40ba-9964-4abda778e61a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 887, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000887__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-888-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 888, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 889, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1210", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "889", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42bdcb94-ad66-4006-bf6b-1beb21eed3d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 888, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000888__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-889-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 889, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 890, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1211", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "890", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d274c00e-42ab-42a5-b50d-b471b7fadda9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 889, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000889__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-890-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 890, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 891, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1212", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "891", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ea049f0-19b1-428d-82ae-e77eed9ad78a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 890, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000890__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-891-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 891, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 892, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1213", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "892", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ece29baf-823d-4e8f-bc7d-09765afe00ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 891, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000891__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-892-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 892, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 893, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1214", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "893", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db733672-74f2-41ff-a187-00a1feaeff33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 892, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000892__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-893-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 893, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 894, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1215", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "894", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b6ffdb9-df33-44d5-b66c-1185f3925578", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 893, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000893__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-894-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 894, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 895, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1216", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "895", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e56ceba-b45f-4880-a354-cc09389a6b79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 894, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000894__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-895-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 895, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 896, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1217", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "896", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fcf7796-f8bc-4132-88ae-03a5311433f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 77940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 895, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000895__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-896-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 896, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 897, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1218", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "897", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d40c81f-cad2-4b22-acab-2e7079cdd8ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 896, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000896__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-897-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 897, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 898, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1219", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "898", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbd0bf56-d5d4-488f-831b-3ddc5a99c01c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 897, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000897__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-898-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 898, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "77984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 899, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1220", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "899", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4ea85b3-0887-4318-aa18-d89b5769a660", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 898, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000898__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-899-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 899, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 900, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1221", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "900", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "935c74aa-ffb0-4a61-9774-30dd27663fd0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 899, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000899__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-900-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 900, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 901, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1222", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "901", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "114c70fe-a95a-4d0c-94b0-d94e36c08c14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 900, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000900__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-901-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 901, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 902, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1223", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "902", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc469b97-2dbc-417a-be85-95bc746b6aaa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 901, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000901__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-902-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 902, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 903, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1224", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "903", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d81be63c-d638-4a30-8b7a-bdf74ca95326", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 902, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000902__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-903-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 903, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 904, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1225", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "904", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87852e48-44db-4b11-a5a4-4c942a1b80c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 903, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000903__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-904-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 904, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 905, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1226", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "905", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f866bdcc-047e-47af-bffe-43df7e05753f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 904, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000904__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-905-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 905, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 906, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1227", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "906", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "210a5af3-15d7-4efd-a384-6f8ecadb7c1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 905, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000905__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-906-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 906, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 907, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1228", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "907", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c2bfed4-e3c2-4c8f-a016-5af2aeb6ec58", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 906, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000906__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-907-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 907, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 908, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1229", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "908", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc4bb1e7-7c3d-469c-b27b-383abddc1728", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 78986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 907, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000907__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-908-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 908, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 909, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1230", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "909", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9360970-4b89-4698-874c-e67a72eedf7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 908, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000908__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-909-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 909, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "78937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 910, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1231", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "910", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c727a860-de6f-4872-8dad-a74d48f3dc90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 909, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000909__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-910-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 910, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 911, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1232", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "911", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62d8fba4-2616-42a8-8239-d64dad282844", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 910, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000910__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-911-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 911, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 912, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1233", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "912", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0ea9e42-7859-4a2f-9854-168be4f62c0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 911, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000911__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-912-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 912, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 913, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1234", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "913", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21974fbd-9da6-49a4-8546-8a3b4cb24d8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 912, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000912__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-913-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 913, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 914, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1235", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "914", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b42d4ab-6afd-4619-8d5d-7f01684473e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 913, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000913__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-914-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 914, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 915, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1236", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "915", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72988b52-de6f-4dd7-b5b9-f6874a8c3f7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 914, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000914__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-915-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 915, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 916, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1237", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "916", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a7e007e-8f1f-494d-b6de-a3133d1fea4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 915, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000915__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-916-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 916, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 917, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1238", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "917", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2eb88e3d-3a4d-4070-abac-76d74587c0df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 916, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000916__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-917-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 917, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 918, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1239", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "918", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb3fb878-23d0-4b68-946f-bef96e1c3ead", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 917, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000917__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-918-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 918, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 919, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1240", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "919", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c38f2578-95f5-430a-a01b-1cf8de039ed3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 79940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 918, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000918__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-919-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 919, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 920, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1241", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "920", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e5763cc-b4ef-4a1c-af02-7e5bdfa54188", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 919, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000919__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-920-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 920, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 921, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1242", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "921", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b5318a5-3b83-4915-a36e-d09cfcb8bb8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 920, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000920__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-921-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 921, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "79984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 922, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1243", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "922", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a48cdd5-def9-44b0-91c7-a09aad518527", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 921, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000921__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-922-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 922, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 923, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1244", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "923", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8db6ab58-ce5f-4a79-8a26-a9ab0b407778", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 922, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000922__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-923-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 923, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 924, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1245", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "924", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9547621-bac6-458c-9ce1-a26946000697", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 923, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000923__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-924-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 924, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 925, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1246", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "925", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15f3addc-a5f8-48ad-b171-19842c894081", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 924, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000924__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-925-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 925, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 926, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1247", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "926", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "126b2551-d8d4-440a-a870-2786257e768a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 925, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000925__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-926-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 926, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 927, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1248", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "927", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f233150-aabf-450d-8782-bb5918f0101c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 926, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000926__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-927-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 927, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 928, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1249", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "928", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c46c88d-8774-4fb6-a7b6-9f4eaad957d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 927, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000927__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-928-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 928, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 929, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1250", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "929", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c44c59c5-ecd7-4c79-b35c-8bc50ca7ccef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 928, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000928__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-929-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 929, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 930, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1251", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "930", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9e2f955-1c0b-41a2-a577-5fe4204e47cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 929, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000929__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-930-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 930, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 931, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1252", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "931", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7be33b49-0b20-498e-b75d-f8574cd4079c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 80986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 930, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000930__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-931-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 931, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 932, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1253", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "932", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4069503c-f312-438c-be34-19e4c455b8e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 931, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000931__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-932-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 932, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "80937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 933, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1254", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "933", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea7092aa-df78-4b23-9bd5-ed21658082e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 932, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000932__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-933-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 933, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 934, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1255", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "934", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f82efac0-4623-4991-bafe-423e376444ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 933, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000933__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-934-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 934, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 935, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1256", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "935", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b543c475-c83a-4dbd-b12e-0492e4bfdaed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 934, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000934__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-935-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 935, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 936, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1257", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "936", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "293edd71-6ad1-4b08-99dc-cb3227224140", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 935, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000935__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-936-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 936, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 937, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1258", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "937", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df049ebb-8517-4b22-9bb5-eb386e686fa0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 936, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000936__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-937-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 937, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 938, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1259", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "938", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46aafa6c-e28b-44e3-bf6b-f1f95ef05edd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 937, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000937__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-938-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 938, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 939, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1260", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "939", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efea1f2e-abf6-4a63-b7e8-fcc8b4043b36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 938, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000938__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-939-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 939, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 940, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1261", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "940", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cde27eca-4fd8-4623-a48c-b36319644866", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 939, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000939__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-940-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 940, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 941, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1262", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "941", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c72ba4f2-6086-438e-9d0a-b907b51961dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 940, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000940__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-941-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 941, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 942, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1263", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "942", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b4097c6-dbea-4cb7-be52-3993b1e540dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 81940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 941, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000941__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-942-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 942, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 943, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1264", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "943", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "916da7ac-50db-4266-9dac-75efc489bee2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 942, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000942__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-943-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 943, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 944, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1265", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "944", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f699022e-5630-4cd6-b59c-0871e7b3a646", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 943, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000943__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-944-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 944, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "81968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 945, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1266", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "945", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c61023d0-9701-466a-8361-3cd81f2c3aa0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 944, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000944__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-945-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 945, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 946, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1267", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "946", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84f54847-2564-41af-bf62-a9dd946ccf13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 945, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000945__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-946-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 946, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 947, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1268", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "947", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd7a017b-e0c8-4c21-884e-20d778c55a3d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 946, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000946__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-947-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 947, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 948, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1269", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "948", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e5c19f4-2122-4b88-b8d6-5da6bfa13a83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 947, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000947__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-948-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 948, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 949, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1270", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "949", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40012328-80f6-400e-90f9-69a72a594561", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 948, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000948__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-949-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 949, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 950, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1271", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "950", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96d66171-626b-4ec0-b158-bdca4c94d307", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 949, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000949__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-950-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 950, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 951, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1272", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "951", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "445980a9-403d-4743-afbd-6070c9a7436d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 950, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000950__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-951-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 951, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 952, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1273", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "952", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ddbf71b-6877-4bc2-a71e-b0d858bd16c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 951, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000951__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-952-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 952, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 953, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1274", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "953", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6825b9eb-24c8-495d-adc5-b4e966b49e59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 952, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000952__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-953-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 953, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 954, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1275", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "954", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf3a48e7-d8e0-40f5-a664-bb528babbf8c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 82971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 953, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000953__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-954-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 954, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 955, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1276", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "955", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fcfe0eac-6f6b-4e0c-b788-ba2352968e9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 954, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000954__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-955-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 955, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "82921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 956, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1277", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "956", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7046428f-f243-46cb-87fd-6576273fe474", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 955, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000955__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-956-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 956, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 957, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1278", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "957", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a503ae2d-ba60-4bed-88d7-68140272da16", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 956, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000956__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-957-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 957, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 958, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1279", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "958", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70bf3e0e-4b45-4b76-bbb6-cb0897245aa5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 957, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000957__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-958-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 958, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 959, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1280", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "959", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1def195-1245-472f-806e-640455967108", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 958, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000958__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-959-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 959, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 960, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1281", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "960", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "642451a2-8f42-4b4f-992a-be782996a00f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 959, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000959__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-960-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 960, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 961, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1282", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "961", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d777162f-e1f8-406b-abc2-a5de59e262d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 960, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000960__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-961-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 961, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 962, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1283", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "962", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82370f2a-d74f-442d-967c-e8cde4a5b38a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 961, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000961__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-962-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 962, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 963, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1284", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "963", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe9630d4-bb43-4dfb-9d7f-1a5c006583f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 962, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000962__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-963-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 963, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 964, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1285", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "964", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1901f2ac-29da-4169-bd5a-cb7b9308629f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 963, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000963__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-964-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 964, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 965, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1286", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "965", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd5c4286-3057-45dc-b762-15b1e7b0f318", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 83924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 964, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000964__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-965-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 965, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 966, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1287", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "966", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c48023f6-2214-432d-870d-342a0d7e882c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 965, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000965__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-966-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 966, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 967, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1288", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "967", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d44153e-d0f7-4cf9-9b30-306cca1a3b52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 966, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000966__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-967-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 967, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "83968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 968, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1289", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "968", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "611b6e73-dcef-4f34-a8a0-caccb007c7f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 967, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000967__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-968-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 968, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 969, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1290", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "969", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "588015ed-1e4e-476e-8de5-d4f497a92d77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 968, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000968__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-969-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 969, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 970, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1291", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "970", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4930a46-6f3d-4e28-886a-dcbeef8e0602", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 969, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000969__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-970-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 970, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 971, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1292", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "971", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "332ad444-14ab-4287-836d-8d797687628e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 970, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000970__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-971-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 971, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 972, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1293", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "972", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c1f4a70-f05f-4602-a6cd-37b87cfa9221", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 971, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000971__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-972-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 972, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 973, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1294", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "973", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21dca631-9a88-4ad4-834e-f470ceab4c1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 972, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000972__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-973-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 973, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 974, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1295", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "974", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76faf922-c1a7-4cd5-a81d-9790a4ba556c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 973, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000973__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-974-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 974, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 975, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1296", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "975", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae6129f1-2aed-4bea-b3c3-82b93733a868", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 974, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000974__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-975-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 975, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 976, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1297", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "976", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b35ce903-0285-47c4-b287-7bd8f3986eb5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 975, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000975__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-976-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 976, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 977, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1298", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "977", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70d38da8-0f5c-44b9-80f8-ff243ae50245", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 84971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 976, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000976__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-977-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 977, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 978, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1299", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "978", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12bc70ae-00b0-4da2-8b63-f0425cea6705", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 977, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000977__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-978-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 978, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "84921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 979, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1300", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "979", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10a30ef3-7e11-4de1-addb-f05ce1402e86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 978, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000978__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-979-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 979, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 980, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1301", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "980", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d3e13b2-a898-45ae-a2ff-8fc932264e8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 979, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000979__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-980-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 980, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 981, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1302", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "981", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a887d8e-bb15-4ea7-b942-2bd26c308b2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 980, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000980__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-981-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 981, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 982, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1303", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "982", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c9978a8-d576-49c0-bc32-b97aad15f8b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 981, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000981__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-982-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 982, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 983, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1304", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "983", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0b0730c-e6cd-4f6f-98c8-77e7f93f87dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 982, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000982__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-983-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 983, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 984, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1305", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "984", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b60c9606-00bc-491b-a2f1-49dd4c5aa7e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 983, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000983__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-984-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 984, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 985, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1306", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "985", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f0bb8b3-c45f-48b1-b2d8-4b2f32af3c3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 984, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000984__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-985-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 985, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 986, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1307", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "986", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c10b1513-2fbe-46d7-8b7e-13f4750cdc62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 985, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000985__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-986-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 986, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 987, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1308", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "987", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ee39a9f-a376-47c4-944d-2728d694f7b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 986, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000986__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-987-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 987, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 988, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1309", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "988", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d1ec5f0-ddcb-4860-a639-078f7e9ec0db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 85924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 987, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000987__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-988-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 988, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 989, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1310", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "989", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38d0e7bf-589a-4627-813c-5604732af7db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 988, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000988__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-989-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 989, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 990, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1311", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "990", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f02829a2-379d-4300-b727-72b215dccb7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 989, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000989__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-990-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 990, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "85968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 991, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1312", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "991", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e74556f-20d9-4ac4-a141-711a3e16da4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 990, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000990__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-991-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 991, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 992, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1313", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "992", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d580bece-6450-44d5-bda1-abeb402dfbee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 991, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000991__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-992-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 992, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 993, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1314", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "993", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27b3646f-dc5d-4afa-a88f-15faa2e3da0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 992, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000992__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-993-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 993, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 994, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1315", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "994", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19228319-1ac9-4ecd-8a87-598cc61f2fdd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 993, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000993__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-994-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 994, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 995, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1316", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "995", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b54f72b2-1e04-4677-a339-bb435c610005", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 994, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000994__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-995-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 995, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 996, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1317", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "996", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a0a016b-216a-4a1c-9bdf-6a23f57a3a71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 995, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000995__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-996-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 996, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 997, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1318", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "997", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4af3ec65-d572-442f-b3c8-7d943f1cd02f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 996, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000996__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-997-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 997, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 998, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1319", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "998", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "118c29ee-2e3a-499e-a90e-5873243a5a10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 997, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000997__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-998-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 998, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 999, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1320", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "999", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0dcd1fa-ee39-41b5-9fd4-d03322bf5427", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 998, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000998__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-999-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 999, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1000, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1321", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1000", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f34bb3c-5509-4aef-97f0-846efc84c606", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 86971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 999, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000999__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1000-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1000, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1001, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1322", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1001", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b457b178-ad3a-46a4-b33a-0d116ae5e78a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1000, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1001-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1001, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "86921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1002, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1323", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1002", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16c933e1-a837-44db-9b76-331dfc63e09d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1001, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001001__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1002-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1002, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1003, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1324", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1003", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05640a45-4581-48b3-97f6-03b369b70181", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1002, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001002__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1003-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1003, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1004, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1325", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1004", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c0dcb36-cf03-4133-b646-805381e47707", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1003, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001003__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1004-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1004, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1005, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1326", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1005", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da3fce59-7953-4fa8-a822-45eeac3a9dfe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1004, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001004__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1005-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1005, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1006, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1327", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1006", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b2a18b3-1e53-4567-b067-4a31bca2ae4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1005, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001005__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1006-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1006, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1007, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1328", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1007", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb7605f3-bb66-4f02-8573-4ddea2774abe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1006, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001006__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1007-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1007, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1008, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1329", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1008", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9192fe05-eda9-4902-bfcb-72bb2a73a7a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1007, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001007__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1008-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1008, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1009, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1330", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1009", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "298a0230-ecc0-43b0-af17-2996ad36669c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1008, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001008__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1009-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1009, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1010, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1331", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1010", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ddccb20f-1cdf-4afe-b755-adb38e40edbc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1009, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001009__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1010-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1010, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1011, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1332", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1011", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f257578-7f2b-4aee-aea9-68bb30407fc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 87924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1010, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001010__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1011-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1011, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1012, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1333", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1012", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2595826f-090c-4a46-abf3-e2ed973ef1fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1011, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001011__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1012-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1012, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1013, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1334", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1013", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9cb59cf4-b656-485b-9c6b-2634a0689df2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1012, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001012__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1013-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1013, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "87953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1014, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1335", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1014", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a695dd2f-e051-4c0c-b50f-895fb7c28c1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1013, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001013__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1014-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1014, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1015, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1336", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1015", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2681f6f7-dd47-40ca-a564-522ded5092f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1014, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001014__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1015-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1015, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1016, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1337", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1016", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02093122-ca9a-42de-b1a3-633bea7f0b9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1015, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001015__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1016-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1016, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1017, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1338", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1017", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "514d449b-389a-4103-86af-fe0b02f07442", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1016, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001016__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1017-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1017, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1018, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1339", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1018", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b392dea0-1aae-4cb2-8488-55eeb3f7acd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1017, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001017__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1018-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1018, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1019, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1340", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1019", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6264f457-7586-413b-b9fd-1da7c2a6edb3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1018, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001018__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1019-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1019, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1020, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1341", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1020", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce346e97-bb5b-4c6c-8300-14c39f9a8ff7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1019, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001019__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1020-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1020, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1021, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1342", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1021", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5baa348b-0b7b-4103-810a-b4c40d50d1c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1020, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001020__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1021-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1021, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1022, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1343", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1022", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38b89b3e-f45a-4f69-ae0e-a6ef1e5a7cb5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1021, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001021__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1022-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1022, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1023, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1344", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1023", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90b228d4-edc7-4c1c-a919-75c07e79359b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 88955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1022, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001022__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1023-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1023, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1024, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1345", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1024", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef760841-d28a-4273-84da-1ccc41619047", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1023, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001023__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1024-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1024, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "88906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1025, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1346", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1025", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "368c74b2-e7bc-4c35-a0e2-1b261d746b60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1024, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001024__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1025-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1025, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1026, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1347", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1026", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8e99c6f-c106-40e9-a078-a23743ca913f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1025, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001025__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1026-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1026, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1027, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1348", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1027", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c131d002-eb07-4d4c-bc63-14a639d6d9a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1026, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001026__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1027-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1027, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1028, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1349", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1028", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87f6b9fa-19cf-4556-a60d-3c9889c5b300", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1027, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001027__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1028-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1028, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1029, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1350", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1029", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "340ebbb7-5068-4a69-904a-12883ac66008", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1028, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001028__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1029-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1029, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1030, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1351", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1030", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c02a118-8a9e-478f-8a0e-3f0f0d119736", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1029, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001029__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1030-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1030, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1031, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1352", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1031", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01c7dd84-cd8a-4969-87c9-e8a591c6d5e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1030, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001030__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1031-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1031, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1032, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1353", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1032", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3fff6e6-09c1-4a48-98c8-74f19a52fe13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1031, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001031__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1032-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1032, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1033, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1354", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1033", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "305b3e5c-21ff-4d80-8979-5944da7da87d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1032, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001032__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1033-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1033, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1034, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1355", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1034", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39d8056d-dcd0-42f8-b05a-b40cdf3e1b04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 89908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1033, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001033__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1034-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1034, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1035, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1356", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1035", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "970dd78d-4838-4dd5-aa77-6edbd9b32471", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1034, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001034__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1035-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1035, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1036, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1357", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1036", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "528eeaae-1c3d-4373-a195-16bb445353fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1035, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001035__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1036-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1036, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "89953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1037, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1358", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1037", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36c4a43c-e11e-42ff-bda7-6f8926abe2e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1036, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001036__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1037-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1037, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1038, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1359", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1038", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57425eae-a41b-4749-9688-86f9906effc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1037, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001037__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1038-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1038, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1039, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1360", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1039", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "708a27c2-d4b3-4ae7-8e4f-605b0a09d542", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1038, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001038__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1039-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1039, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1040, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1361", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1040", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ea0a4d4-6014-4d69-926d-8b5a6bd051b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1039, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001039__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1040-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1040, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1041, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1362", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1041", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab13a1cd-c14a-41a0-bbd0-4e0b8e6a7e83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1040, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001040__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1041-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1041, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1042, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1363", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1042", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd8c1947-8a38-4363-b3b7-e95e02ddc3c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1041, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001041__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1042-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1042, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1043, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1364", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1043", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f5c68e1-67d4-451d-93a0-3e3cc3ea6dab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1042, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001042__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1043-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1043, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1044, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1365", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1044", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c59d9024-916e-45c0-a2a3-fcbdced25f91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1043, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001043__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1044-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1044, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1045, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1366", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1045", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "105b0c89-6b40-4894-a646-d3e0890a9c74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1044, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001044__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1045-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1045, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1046, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1367", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1046", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1abb9e9-c68d-427a-b743-0dc5446c2fc8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 90955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1045, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001045__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1046-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1046, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1047, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1368", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1047", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "866a93a8-e3e1-4578-9163-36784a868492", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1046, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001046__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1047-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1047, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "90906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1048, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1369", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1048", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "318487c2-c606-4afc-91df-cd0bdc67e974", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1047, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001047__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1048-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1048, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1049, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1370", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1049", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e9e50af-08af-4283-9bee-3ff8b58ed0ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1048, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001048__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1049-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1049, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1050, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1371", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1050", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64f98399-fcc0-4d14-936d-188dee834841", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1049, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001049__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1050-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1050, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1051, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1372", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1051", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7ac5645-0188-41c3-9ef1-091004dfc227", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1050, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001050__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1051-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1051, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1052, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1373", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1052", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a05f9a3-650f-419d-a344-f667826620e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1051, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001051__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1052-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1052, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1053, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1374", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1053", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "116e81e9-6918-44f7-9d29-45da28797b6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1052, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001052__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1053-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1053, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1054, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1375", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1054", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6eb3d55b-3b1c-4396-a536-30a368a0b601", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1053, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001053__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1054-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1054, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1055, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1376", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1055", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c72c1e53-3393-4216-a4ae-6e532e6f6bb8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1054, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001054__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1055-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1055, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1056, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1377", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1056", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b00ef5a-2423-41fe-818e-5bf509f290db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1055, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001055__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1056-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1056, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1057, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1378", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1057", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3c248ae-a1d4-4dbe-9ae0-42a2114038a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 91908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1056, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001056__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1057-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1057, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1058, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1379", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1058", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6098f060-a6df-40a3-b6a1-ab87971f9ce2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1057, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001057__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1058-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1058, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1059, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1380", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1059", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98650805-2270-4f90-bffa-f3a12d16ac46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1058, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001058__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1059-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1059, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "91953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1060, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1381", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1060", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07bce454-1b24-4b35-a7dd-c1610cda6e6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1059, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001059__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1060-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1060, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1061, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1382", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1061", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a5b7ad1-fc3b-4656-99de-1ab3f39beb26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1060, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001060__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1061-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1061, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1062, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1383", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1062", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c25905dd-4fdd-4564-a04a-af182cfbf713", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1061, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001061__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1062-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1062, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1063, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1384", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1063", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e3a7a0e-f4ca-43a1-a816-491e2be8a986", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1062, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001062__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1063-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1063, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1064, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1385", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1064", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33320bc8-78b3-47d8-9a82-f37b944faec9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1063, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001063__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1064-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1064, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1065, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1386", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1065", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bc0e4f2-9774-4e0b-bff8-f318ba02672f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1064, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001064__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1065-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1065, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1066, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1387", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1066", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42c85f50-4b2b-46f3-b345-77504ed1fc69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1065, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001065__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1066-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1066, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1067, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1388", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1067", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d054b835-683c-4beb-9fbd-fd359a22a144", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1066, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001066__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1067-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1067, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1068, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1389", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1068", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bde5c888-257d-4336-b4fa-9479bea5caaf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1067, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001067__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1068-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1068, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1069, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1390", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1069", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c374c09-1324-438d-8e2c-71dd111098c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 92955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1068, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001068__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1069-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1069, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1070, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1391", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1070", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86e6a8ec-0e1f-445d-8e27-582513ba5925", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1069, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001069__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1070-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1070, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1071, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1392", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1071", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a182ac21-454a-4fd2-84ea-0bef4055cc03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1070, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001070__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1071-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1071, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "92984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1072, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1393", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1072", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b34ad139-c8f8-4b14-b5a5-6f9028b83b5f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1071, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001071__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1072-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1072, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1073, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1394", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1073", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6da71e3-b217-4bc3-be9b-81a0af8cd77a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1072, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001072__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1073-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1073, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1074, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1395", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1074", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3911ac83-6986-4baf-90d0-df2b039214fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1073, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001073__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1074-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1074, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1075, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1396", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1075", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6ee9b52-aec0-4e8d-9549-bdc5704f6104", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1074, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001074__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1075-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1075, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1076, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1397", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1076", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d5c211e-6f87-42f4-9ec7-497a9e61eb38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1075, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001075__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1076-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1076, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1077, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1398", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1077", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69fb2927-acf8-4e04-9eec-74fba96d8c1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1076, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001076__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1077-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1077, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1078, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1399", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1078", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "738eca1a-a718-4e00-90b9-c1a49f493ab3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1077, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001077__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1078-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1078, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1079, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1400", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1079", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebae29a7-a9ed-4022-9a15-3991cda7683f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1078, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001078__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1079-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1079, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1080, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1401", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1080", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37051834-2d3b-4d79-aba1-5ac2d05ddeca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1079, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001079__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1080-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1080, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1081, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1402", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1081", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0dc28961-f8b0-4bb9-bb6b-e43f32fed061", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 93986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1080, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001080__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1081-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1081, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1082, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1403", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1082", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f0d4386-c16d-4d4f-90fd-2808d6700b9c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1081, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001081__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1082-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1082, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "93937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1083, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1404", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1083", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b130c119-c845-4538-9cd2-567a7497a881", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1082, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001082__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1083-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1083, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1084, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1405", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1084", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bbcde45-bae5-4c1c-9a1c-d26dc61b4cbd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1083, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001083__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1084-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1084, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1085, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1406", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1085", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a71e5646-5db0-4de4-8e21-078a48a61927", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1084, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001084__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1085-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1085, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1086, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1407", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1086", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8108c51a-1eca-4acb-9c0f-683e2569e248", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1085, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001085__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1086-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1086, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1087, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1408", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1087", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4531f38c-6ea7-4e72-a9db-b1309cb70646", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1086, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001086__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1087-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1087, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1088, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1409", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1088", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab43d35a-3327-40e8-a5de-d8a1581a148d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1087, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001087__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1088-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1088, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1089, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1410", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1089", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d762c908-ac84-4498-91cf-22a597f2ebc8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1088, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001088__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1089-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1089, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1090, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1411", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1090", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "048181f6-30b5-4fc5-8ff6-f2a5d450f539", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1089, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001089__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1090-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1090, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1091, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1412", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1091", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5695f03e-7568-44cb-b262-14e35a6ba57a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1090, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001090__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1091-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1091, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1092, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1413", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1092", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "475c9f16-81dd-4390-9513-2c7fcc9f6f6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1091, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001091__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1092-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1092, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1093, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1414", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1093", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d7342c2-d5e2-4401-8133-d3260750841f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1092, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001092__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1093-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1093, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1094, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1415", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1094", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f44c3b4-b3b7-4fba-ab0a-ddcfb4e9261b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1093, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001093__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1094-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1094, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "94984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1095, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1416", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1095", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62e2d19c-80d7-4c4f-b641-8839bcaff48c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1094, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001094__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1095-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1095, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1096, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1417", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1096", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48e3e6f6-47a6-49a8-b79e-3a7e11c1ca52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1095, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001095__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1096-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1096, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1097, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1418", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1097", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86a74d54-f6cb-41b0-bbad-5ebba6691623", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1096, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001096__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1097-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1097, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1098, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1419", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1098", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "887bd154-9902-42f6-b2d4-a4efc900cf36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1097, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001097__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1098-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1098, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1099, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1420", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1099", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "032b3455-308b-41e9-8bad-da1aff930cd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1098, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001098__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1099-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1099, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1100, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1421", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1100", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "465e54d1-e946-4c24-a034-9dc4c411f747", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1099, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001099__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1100-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1100, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1101, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1422", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1101", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe80c87c-e3eb-4118-ac4a-7eefe5738f74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1100, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001100__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1101-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1101, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1102, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1423", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1102", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89f8fbb1-e83f-4328-a59a-d41204a5fb29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1101, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001101__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1102-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1102, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1103, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1424", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1103", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fb97dc8-3887-4a6a-9796-597e52fbcd50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1102, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001102__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1103-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1103, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1104, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1425", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1104", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25fe6437-c737-4efd-bce1-823eecca5435", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 95986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1103, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001103__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1104-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1104, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1105, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1426", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1105", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a85f7b00-4a8f-4819-8e99-58e8c88bbccd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1104, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001104__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1105-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1105, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "95937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1106, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1427", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1106", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a990e2f7-5eea-4121-812d-24e3a71d8781", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1105, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001105__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1106-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1106, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1107, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1428", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1107", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "179232ff-6630-417e-a639-df71b018072e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1106, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001106__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1107-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1107, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1108, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1429", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1108", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4785b7b6-8c55-4b30-8353-fd72be5941aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1107, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001107__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1108-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1108, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1109, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1430", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1109", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a96d7d8c-df16-43cd-9c15-dbe3e83aba1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1108, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001108__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1109-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1109, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1110, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1431", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1110", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e59913ea-59aa-4442-a1c0-5a7521b450d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1109, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001109__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1110-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1110, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1111, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1432", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1111", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12b07254-698f-421c-b44d-3ca030299481", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1110, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001110__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1111-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1111, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1112, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1433", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1112", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c13f13a-be08-4f25-8a13-420cc1ffe64e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1111, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001111__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1112-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1112, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1113, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1434", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1113", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af05917c-43ac-4f13-b5c6-23f3e1886ee9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1112, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001112__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1113-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1113, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1114, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1435", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1114", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8160ecf6-aae0-492f-aa96-2bbb99214c23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1113, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001113__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1114-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1114, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1115, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1436", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1115", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e671f66a-e561-4a8b-a02e-64c95bc0e18a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 96940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1114, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001114__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1115-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1115, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1116, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1437", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1116", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d63b2c3-e908-41ae-baaf-86bb1b89f4a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1115, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001115__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1116-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1116, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1117, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1438", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1117", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d6a8415-4681-434e-bccf-ba1bc734f2ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1116, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001116__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1117-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1117, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "96984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1118, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1439", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1118", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72539e10-67da-4a31-b193-cb12eab1096f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1117, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001117__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1118-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1118, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1119, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1440", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1119", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26c1bf35-95f7-47f7-8be7-4e100d7995d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1118, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001118__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1119-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1119, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1120, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1441", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1120", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6033f01-4e16-4b7d-8dd5-14529d994f45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1119, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001119__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1120-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1120, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1121, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1442", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1121", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cdd35e8-90f2-45f0-8781-8580da737501", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1120, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001120__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1121-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1121, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1122, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1443", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1122", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c213945-a449-489a-8402-ab52858b67aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1121, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001121__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1122-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1122, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1123, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1444", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1123", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f9bfec4-317b-4adc-8899-2553e819aa40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1122, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001122__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1123-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1123, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1124, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1445", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1124", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a0ba72e-6ab1-4edd-bd34-07762464ab37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1123, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001123__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1124-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1124, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1125, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1446", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1125", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e2300dd-0886-4cde-911e-6bf2af508d8c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1124, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001124__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1125-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1125, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1126, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1447", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1126", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "924ccc4c-3cc0-417d-80e9-da6f14ef32ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1125, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001125__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1126-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1126, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1127, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1448", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1127", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bea0a81-e20a-44a6-9719-a8eacaab8d99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 97986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1126, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001126__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1127-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1127, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1128, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1449", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1128", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4611e242-3669-4fdb-8246-af2f89232c56", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1127, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001127__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1128-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1128, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "97937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1129, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1450", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1129", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92d9e2e9-f426-44e1-b256-3c6808d49b7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1128, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001128__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1129-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1129, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1130, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1451", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1130", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fdcd274-843d-40b8-91b8-574ed0580e77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1129, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001129__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1130-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1130, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1131, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1452", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1131", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "643858ad-9aa9-448d-9d30-a6087eb3f273", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1130, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001130__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1131-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1131, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1132, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1453", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1132", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d2b7d69-19e4-48a6-a64f-80aa251988c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1131, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001131__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1132-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1132, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1133, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1454", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1133", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5d4d596-afe9-4b5c-8556-4ef25e03a878", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1132, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001132__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1133-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1133, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1134, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1455", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1134", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af48e01d-b291-4f00-9fb3-0ef431ecc280", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1133, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001133__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1134-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1134, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1135, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1456", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1135", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65481751-fa63-48fa-9623-0817b0e01337", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1134, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001134__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1135-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1135, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1136, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1457", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1136", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05664f75-3425-4c6b-8a13-77c7ecf8de2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1135, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001135__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1136-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1136, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1137, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1458", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1137", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5d4ba20-b45f-43d2-97dc-ab7703fd9cc4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1136, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001136__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1137-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1137, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1138, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1459", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1138", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18648d0e-66de-43b6-b15d-2c6fe041c27e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 98940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1137, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001137__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1138-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1138, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1139, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1460", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1139", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "321fd088-a243-4075-8496-99c19d2d6f94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1138, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001138__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1139-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1139, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1140, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1461", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1140", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17257ea8-353a-4be8-be2f-570812976982", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1139, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001139__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1140-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1140, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "98968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1141, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1462", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1141", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "293d1129-05b7-40b5-a38e-fb75ef6244ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1140, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001140__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1141-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1141, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1142, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1463", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1142", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cebca0c-c10c-4dd6-8dc5-7937e4f151f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1141, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001141__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1142-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1142, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1143, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1464", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1143", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85f99172-5cac-4a7f-9d9d-39782d357058", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1142, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001142__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1143-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1143, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1144, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1465", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1144", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "596d21f5-95c7-4e75-9a26-4d4559730968", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1143, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001143__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1144-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1144, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1145, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1466", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1145", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cff00b3-e7cc-478e-a774-b4aa33c22ae9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1144, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001144__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1145-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1145, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1146, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1467", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1146", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c47f69ae-960a-48ea-a219-04050f961ad0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1145, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001145__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1146-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1146, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1147, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1468", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1147", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3426e61-3770-4d7e-bc04-e1ac69e318e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1146, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001146__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1147-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1147, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1148, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1469", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1148", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "622d0b8e-a53d-46be-aa1d-707ad20678a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1147, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001147__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1148-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1148, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1149, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1470", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1149", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11e933d1-5620-49bd-9a48-7a7819e26da2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1148, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001148__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1149-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1149, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1150, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1471", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1150", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f067810-ae1d-4b27-a01d-47d09b30730e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 99971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1149, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001149__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1150-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1150, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1151, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1472", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1151", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06de149f-f839-4cf3-a744-67e7c3ebde05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1150, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001150__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1151-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1151, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "99921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1152, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1473", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1152", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d1c19c2-04e5-47e3-a65d-394b2ce436a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1151, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001151__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1152-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1152, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1153, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1474", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1153", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37cef355-c8e6-484a-9d65-4c5c3fd19537", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1152, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001152__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1153-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1153, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1154, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1475", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1154", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a681f50-edf5-467a-b205-38f535b0fe4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1153, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001153__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1154-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1154, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1155, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1476", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1155", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02ad8199-023d-48d3-b95d-2e0028f868db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1154, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001154__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1155-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1155, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1156, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1477", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1156", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90377274-9259-43ef-9e38-5cfc2b7b9c3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1155, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001155__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1156-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1156, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1157, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1478", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1157", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22d0839f-3bd1-42e1-9e5f-aebe1be54835", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1156, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001156__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1157-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1157, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1158, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1479", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1158", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87167734-c7e2-4c99-b449-f6583dad85dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1157, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001157__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1158-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1158, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1159, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1480", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1159", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58443f43-d23c-4363-b557-004b632d2046", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1158, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001158__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1159-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1159, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1160, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1481", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1160", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b7d0200-a6bb-456d-aece-d5091638645a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1159, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001159__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1160-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1160, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1161, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1482", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1161", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4bed25b-d171-4a53-a21b-6039870d52ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 100924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1160, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001160__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1161-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1161, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1162, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1483", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1162", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4aae040-bf16-44f3-9de4-4bc990448f2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1161, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001161__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1162-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1162, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1163, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1484", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1163", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bfaf373-0f52-4b7e-860c-06fc984946fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1162, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001162__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1163-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1163, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "100968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1164, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1485", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1164", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "582ce880-e8cc-4d7f-a84c-20ca5d8af952", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1163, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001163__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1164-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1164, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1165, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1486", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1165", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "369c5cb3-8f14-4443-9629-c1e28d3d7364", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1164, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001164__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1165-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1165, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1166, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1487", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1166", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19ebc8fa-47fc-4599-ba5a-c6bfb38b7e49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1165, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001165__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1166-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1166, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1167, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1488", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1167", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "660e220d-6d83-42c4-9f5d-112b990c9eae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1166, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001166__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1167-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1167, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1168, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1489", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1168", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ada108f-9e56-4fec-8b92-2344e5ea1838", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1167, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001167__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1168-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1168, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1169, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1490", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1169", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a477deea-3305-4dce-9a2c-8e1fcbb17a74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1168, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001168__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1169-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1169, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1170, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1491", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1170", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b63d623a-40d4-47df-8a6e-a79aed653a62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1169, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001169__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1170-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1170, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1171, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1492", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1171", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68a92f72-6acc-42cd-95f7-c298d74227b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1170, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001170__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1171-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1171, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1172, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1493", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1172", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1734fdbb-0316-4d4f-b50d-6c9d617ad1d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1171, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001171__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1172-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1172, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1173, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1494", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1173", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "041db4e3-8c8c-4c53-a665-7329f661f744", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 101971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1172, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001172__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1173-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1173, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1174, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1495", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1174", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b366632d-d310-46e9-a7f3-fe79c7094aec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1173, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001173__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1174-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1174, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "101921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1175, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1496", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1175", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c74295e-9dca-4d68-b123-80ab8336f81b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1174, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001174__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1175-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1175, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1176, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1497", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1176", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e154947c-c021-4074-b807-64afe1cc379d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1175, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001175__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1176-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1176, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1177, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1498", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1177", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1eba15f3-5f09-498c-9db8-3b7acbed8f43", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1176, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001176__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1177-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1177, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1178, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1499", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1178", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a648fa4f-8568-41ed-b3a2-dc91b2dab54f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1177, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001177__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1178-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1178, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1179, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1500", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1179", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b760f21-ff69-4ee6-adf4-3b4bebc69316", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1178, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001178__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1179-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1179, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1180, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1501", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1180", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7cd95755-855c-41a2-9552-0b8c5b6dff16", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1179, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001179__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1180-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1180, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1181, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1502", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1181", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e395eb0-762c-4f80-bd57-de9cef3d4d56", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1180, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001180__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1181-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1181, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1182, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1503", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1182", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2a51e4b-3640-4ff3-98fa-0ab1f6b6ce2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1181, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001181__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1182-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1182, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1183, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1504", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1183", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04329a05-4bad-4027-a35b-21c36d783005", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1182, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001182__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1183-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1183, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1184, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1505", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1184", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb42e024-bf3c-4c89-80bc-1550a9a1f16c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 102924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1183, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001183__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1184-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1184, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1185, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1506", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1185", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "989ae876-7fb8-47da-8466-5b4ed9c52efa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1184, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001184__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1185-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1185, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1186, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1507", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1186", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2db6eb2-3e49-4aff-876c-8bd836ed8437", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1185, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001185__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1186-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1186, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "102968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1187, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1508", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1187", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f031d242-65b5-4413-8383-d231f52e6241", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1186, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001186__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1187-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1187, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1188, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1509", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1188", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3dcb64f4-d18e-4085-a9ae-a3d67b7e042c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1187, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001187__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1188-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1188, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1189, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1510", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1189", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fbe1767-ada9-4280-b404-faa3b2d8ab66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1188, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001188__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1189-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1189, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1190, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1511", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1190", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "871e714e-6b88-4017-a13b-84294971a74e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1189, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001189__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1190-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1190, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1191, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1512", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1191", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b5fb641-7824-4435-b85a-64d80e593747", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1190, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001190__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1191-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1191, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1192, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1513", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1192", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0dea615-5ce8-4f67-8e8c-9547bacd420d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1191, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001191__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1192-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1192, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1193, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1514", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1193", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4272ef03-984d-4839-b77a-a686483e2151", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1192, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001192__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1193-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1193, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1194, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1515", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1194", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92da9629-466c-410f-b077-69d1af152618", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1193, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001193__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1194-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1194, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1195, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1516", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1195", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fe47d55-367d-4c68-b574-b762d55e7607", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1194, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001194__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1195-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1195, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1196, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1517", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1196", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6735715-9085-40dd-bbe4-4cd9facab786", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 103971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1195, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001195__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1196-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1196, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1197, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1518", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1197", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10cbb142-8940-43b8-ac7a-b1cde1b54361", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1196, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001196__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1197-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1197, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "103921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1198, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1519", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1198", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94f13657-5dc2-4a5c-9049-3e34a685731e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1197, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001197__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1198-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1198, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1199, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1520", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1199", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75714c19-13cf-44f2-98f6-332a482fa3d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1198, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001198__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1199-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1199, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1200, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1521", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1200", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d1cb02f-73aa-4d62-b179-884f58b773e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1199, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001199__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1200-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1200, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1201, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1522", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1201", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2968fbac-6f4d-4d8a-b356-422260379681", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1200, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001200__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1201-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1201, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1202, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1523", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1202", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b68267db-0d01-4e32-bcdc-4e0dad07a6c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1201, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001201__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1202-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1202, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1203, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1524", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1203", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b35b49b-b2ad-48fe-88d7-d4e6818841a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1202, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001202__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1203-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1203, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1204, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1525", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1204", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6038ddce-a84c-4c9f-8c22-6c6df8eb838d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1203, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001203__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1204-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1204, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1205, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1526", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1205", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e160502-7114-4c95-9bb3-5668cd6bf406", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1204, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001204__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1205-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1205, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1206, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1527", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1206", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c07151ce-9555-4435-a00a-5088a12b6461", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1205, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001205__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1206-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1206, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1207, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1528", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1207", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5db30c3f-2456-45f1-9066-a89fc8549540", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 104924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1206, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001206__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1207-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1207, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1208, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1529", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1208", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96e56159-23c1-440a-81e3-944e7dc5767e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1207, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001207__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1208-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1208, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1209, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1530", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1209", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c1e0a6b-e2d1-454f-b001-d8e9a59dc81f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1208, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001208__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1209-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1209, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "104953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1210, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1531", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1210", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dfc07b22-542b-427f-8e66-80d05f097ecf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1209, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001209__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1210-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1210, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1211, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1532", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1211", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10d146c4-09d0-492d-a9d2-ffde86bf1854", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1210, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001210__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1211-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1211, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1212, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1533", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1212", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "129ef8bd-493c-4c35-84a9-4fc07c8d7c54", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1211, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001211__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1212-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1212, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1213, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1534", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1213", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c084157d-d9fa-49f4-af99-8297823aac09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1212, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001212__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1213-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1213, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1214, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1535", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1214", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c35cd090-bf74-4ec3-a72a-fb26efa7bd8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1213, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001213__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1214-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1214, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1215, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1536", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1215", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2dd125d-bb66-4fbe-af90-bd12b124a23e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1214, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001214__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1215-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1215, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1216, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1537", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1216", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43100dc7-1561-4948-a07d-cec768e39b4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1215, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001215__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1216-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1216, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1217, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1538", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1217", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe56c74e-6fa1-41c6-9e49-63470a09c99d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1216, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001216__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1217-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1217, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1218, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1539", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1218", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0bca668-a98f-4bdc-9f9e-363d1236fbd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1217, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001217__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1218-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1218, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1219, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1540", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1219", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3df8034a-74e2-4ee2-b676-5dd6ea79fa98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 105955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1218, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001218__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1219-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1219, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1220, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1541", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1220", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "010cbd30-4586-4574-ad54-3836bcd72622", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1219, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001219__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1220-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1220, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "105921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1221, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1542", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1221", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "585a8509-d96f-461d-b249-82ba9170c08f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1220, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001220__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1221-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1221, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1222, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1543", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1222", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19cd0c6d-85d0-48bb-bfb0-ad570e42ca52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1221, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001221__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1222-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1222, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1223, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1544", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1223", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc2badde-eb44-4c79-accf-88c94359e70e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1222, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001222__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1223-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1223, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1224, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1545", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1224", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5611ff9-36f7-436d-a4e6-478f0c30e1e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1223, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001223__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1224-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1224, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1225, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1546", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1225", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aca3255f-b9f3-409a-9f2d-c7a4a0bcf3ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1224, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001224__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1225-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1225, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1226, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1547", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1226", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c07278ed-b08d-4245-908f-ca44d5a53ae3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1225, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001225__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1226-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1226, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1227, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1548", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1227", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8ca0033-97e9-46f2-acbb-defee201f85b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1226, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001226__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1227-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1227, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1228, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1549", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1228", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dc4f03d-a035-4303-839a-33210c1ac846", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1227, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001227__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1228-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1228, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1229, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1550", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1229", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "965fcb59-fae3-4b7f-a155-478c2058827a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1228, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001228__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1229-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1229, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1230, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1551", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1230", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40a4724f-8f2b-4c72-8dd6-2a073bb2cb85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 106908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1229, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001229__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1230-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1230, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1231, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1552", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1231", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f085c41b-933e-4b0c-b934-1efdabc662c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1230, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001230__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1231-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1231, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1232, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1553", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1232", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc64e81e-881c-451a-b8d2-36ee186fc41d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1231, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001231__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1232-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1232, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "106953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1233, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1554", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1233", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9747ae7-dee7-4ce8-acc4-6923a823665e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1232, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001232__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1233-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1233, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1234, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1555", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1234", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "766d58bf-16b2-4402-9448-6b40831d649b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1233, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001233__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1234-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1234, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1235, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1556", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1235", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a566dba-e11a-4fd8-b6ac-15543c665d8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1234, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001234__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1235-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1235, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1236, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1557", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1236", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed823469-7485-47be-8333-1a5a14682fe5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1235, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001235__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1236-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1236, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1237, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1558", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1237", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08924e0b-64bb-4ecb-964c-512e30c4832c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1236, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001236__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1237-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1237, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1238, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1559", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1238", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fbbf85a-ba28-4835-9eeb-3a7e8aaa122d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1237, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001237__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1238-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1238, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1239, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1560", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1239", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec58fc2f-af40-4e4b-a7b0-3ef5c1951133", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1238, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001238__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1239-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1239, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1240, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1561", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1240", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3d8ebcb-8ac1-4bd3-94a6-3368b549fa0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1239, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001239__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1240-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1240, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1241, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1562", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1241", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20bd899f-3295-4211-a3ac-e0535d9e8e29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1240, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001240__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1241-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1241, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1242, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1563", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1242", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c814fcad-11d8-48be-b708-d0429cd32682", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 107955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1241, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001241__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1242-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1242, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1243, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1564", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1243", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3440346-157a-437f-bf4a-d5db8c761a02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1242, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001242__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1243-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1243, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "107906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1244, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1565", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1244", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f375d4c-abdd-4115-86a7-a2e1391a1f74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1243, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001243__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1244-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1244, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1245, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1566", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1245", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24fcba5f-e556-46df-a31e-acb842c1f7ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1244, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001244__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1245-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1245, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1246, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1567", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1246", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b894939c-bd62-40cf-bb89-bb77b8402d43", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1245, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001245__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1246-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1246, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1247, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1568", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1247", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a9991eb-d99c-455d-8cca-db81b0f0af1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1246, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001246__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1247-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1247, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1248, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1569", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1248", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fcd1493-a8c4-4f8d-bee2-dc97150ab38b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1247, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001247__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1248-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1248, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1249, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1570", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1249", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3e65d59-62da-4eea-9cce-2de2d57b82ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1248, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001248__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1249-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1249, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1250, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1571", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1250", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a972f0e7-0d26-4cda-8a34-13d2cd42e015", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1249, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001249__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1250-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1250, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1251, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1572", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1251", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b26b9869-767c-43cf-8857-3f1593a82b02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1250, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001250__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1251-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1251, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1252, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1573", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1252", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f8f4843-36c9-4696-87ff-b95009e2e9ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1251, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001251__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1252-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1252, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1253, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1574", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1253", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e4dae46-4b77-4776-8850-53f955c25d18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 108908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1252, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001252__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1253-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1253, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1254, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1575", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1254", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d56fd776-a664-4a74-8873-dbf76844820e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1253, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001253__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1254-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1254, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1255, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1576", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1255", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04bff135-37b3-4c84-b46d-9c51186dc895", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1254, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001254__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1255-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1255, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "108953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1256, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1577", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1256", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2e01bd3-288d-41d9-a13a-68a1913b3ef9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1255, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001255__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1256-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1256, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1257, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1578", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1257", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfc06733-851e-4126-9637-3cdf26c6725c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1256, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001256__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1257-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1257, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1258, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1579", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1258", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b71721e-63bd-41fb-a2a8-fc4d2e010140", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1257, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001257__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1258-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1258, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1259, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1580", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1259", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe432506-173c-4787-925c-ce2483607a63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1258, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001258__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1259-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1259, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1260, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1581", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1260", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40a810c6-d192-4d57-b9a6-d00a62139ddb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1259, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001259__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1260-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1260, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1261, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1582", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1261", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "579c7296-ea66-4f75-9ba3-36e4c7408940", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1260, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001260__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1261-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1261, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1262, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1583", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1262", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2e9c527-131e-481d-8585-a1e6a545fd06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1261, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001261__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1262-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1262, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1263, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1584", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1263", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09b90063-1dd5-4bef-8e8e-ef814b0a52b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1262, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001262__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1263-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1263, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1264, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1585", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1264", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd8230cb-0500-40f8-8d8e-bd0ae42bf375", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1263, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001263__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1264-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1264, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1265, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1586", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1265", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09ffeded-4673-4051-810d-4d5de24e5dbc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 109955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1264, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001264__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1265-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1265, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1266, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1587", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1266", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b076b87-f0fb-4a78-8da7-aab5e58ea675", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1265, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001265__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1266-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1266, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1267, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1588", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1267", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eecda603-730e-4dcc-b2bf-d47d54df5e42", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1266, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001266__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1267-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1267, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "109984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1268, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1589", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1268", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ebfa536-e2b2-4309-866b-3765f0b0e285", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1267, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001267__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1268-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1268, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1269, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1590", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1269", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "713c60a4-0768-47a6-b4d7-ceb24a5758ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1268, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001268__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1269-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1269, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1270, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1591", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1270", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28e41a74-d066-48c5-9b73-53ed50788850", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1269, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001269__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1270-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1270, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1271, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1592", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1271", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59014d0d-0391-4239-ac25-b74af5735ee7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1270, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001270__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1271-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1271, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1272, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1593", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1272", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c068ab7-80a2-4a90-8ab0-268f43ad9501", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1271, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001271__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1272-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1272, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1273, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1594", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1273", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2479d3bf-0b23-4fba-8aa9-b2161b31a2d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1272, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001272__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1273-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1273, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1274, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1595", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1274", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "833aab6a-1985-47dd-b71e-4904dfa7a607", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1273, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001273__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1274-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1274, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1275, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1596", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1275", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1ac6f3e-c1f9-4da8-91fc-4fc47a8e1451", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1274, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001274__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1275-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1275, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1276, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1597", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1276", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcfacc84-fb98-4c3b-ae37-19f1da259be7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1275, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001275__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1276-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1276, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1277, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1598", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1277", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "290c1523-639d-45ac-8253-bab773e8185f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 110986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1276, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001276__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1277-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1277, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1278, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1599", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1278", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98b84d7e-fb62-4e27-b81f-e43643cd9a80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1277, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001277__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1278-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1278, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "110937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1279, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1600", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1279", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "554987ab-a6b3-453d-8792-cc3acb32a620", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1278, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001278__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1279-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1279, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1280, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1601", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1280", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e78d032-35e4-4779-9370-511eab7b360d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1279, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001279__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1280-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1280, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1281, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1602", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1281", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0560939-675a-4cde-af60-c6e3a476806b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1280, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001280__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1281-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1281, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1282, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1603", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1282", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01303fac-3bf9-43fb-96c9-605af8171784", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1281, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001281__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1282-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1282, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1283, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1604", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1283", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a76bc86e-93ef-4ee7-9e43-ca2257a02d75", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1282, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001282__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1283-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1283, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1284, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1605", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1284", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "177e4d90-3729-4eee-be9f-b8bda547040c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1283, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001283__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1284-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1284, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1285, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1606", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1285", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23d39e56-df72-442e-b654-d87c28da645a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1284, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001284__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1285-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1285, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1286, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1607", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1286", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01dbb947-2f5a-4e12-a997-6f3fb238e1fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1285, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001285__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1286-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1286, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1287, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1608", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1287", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "234d9aac-c26e-483d-b348-8dcc4a296dbc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1286, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001286__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1287-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1287, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1288, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1609", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1288", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b391215-4ed4-49ee-9c95-632ca0d66777", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 111940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1287, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001287__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1288-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1288, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1289, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1610", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1289", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1233bc0-fcf3-4714-93f4-6876c39a2ad3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1288, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001288__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1289-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1289, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1290, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1611", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1290", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7cdbe9b5-2f22-41bb-9d4e-f6a5385649b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1289, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001289__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1290-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1290, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "111984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1291, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1612", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1291", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c72589c-5e44-4ba8-b1b1-001b92f3184c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1290, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001290__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1291-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1291, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1292, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1613", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1292", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "332d0dd0-8f2d-45e2-824f-5ba15a09e5a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1291, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001291__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1292-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1292, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1293, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1614", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1293", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c3b1c06-0d0f-48d3-907b-0b2941c9b9fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1292, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001292__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1293-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1293, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1294, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1615", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1294", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fffa312b-0a73-480a-965d-f23f040d8637", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1293, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001293__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1294-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1294, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1295, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1616", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1295", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "394cbe47-fcc3-4f89-bcbb-f148e72760ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1294, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001294__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1295-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1295, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1296, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1617", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1296", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4f88f9d-f6ba-48b9-9246-7d90cba40778", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1295, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001295__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1296-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1296, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1297, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1618", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1297", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da8aa2b3-6b76-44a6-9f0e-19018ccd9fbf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1296, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001296__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1297-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1297, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1298, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1619", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1298", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f934921e-1fea-4c88-a623-5a63235a28c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1297, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001297__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1298-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1298, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1299, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1620", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1299", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "519b58d1-69a7-4501-917a-870a592e8839", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1298, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001298__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1299-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1299, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1300, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1621", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1300", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7f23bb5-948f-445a-b2b3-5da3ed8c867a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 112986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1299, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001299__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1300-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1300, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1301, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1622", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1301", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "126f33da-6fdf-4b95-966f-93c631806f68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1300, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001300__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1301-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1301, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "112937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1302, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1623", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1302", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaae0a87-bec7-4325-8b09-9d0e0840b863", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1301, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001301__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1302-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1302, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1303, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1624", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1303", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a648976-5698-4382-afc7-55d1a9ad224b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1302, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001302__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1303-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1303, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1304, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1625", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1304", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f282933-d30c-458a-86f3-5e2a86609ea0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1303, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001303__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1304-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1304, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1305, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1626", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1305", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e95322c-c1cf-431d-8c40-c98f473f38af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1304, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001304__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1305-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1305, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1306, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1627", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1306", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "588e409b-a1db-4cb3-baac-65e08ca06be9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1305, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001305__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1306-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1306, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1307, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1628", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1307", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "481d61a0-d647-46d4-b474-2809d411a02e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1306, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001306__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1307-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1307, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1308, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1629", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1308", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58c0bb18-a068-4733-bae4-443374c41876", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1307, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001307__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1308-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1308, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1309, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1630", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1309", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a1f3a11-e4c1-4f1a-a00f-7dc49fd7b6a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1308, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001308__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1309-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1309, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1310, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1631", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1310", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8712d748-75c4-4b76-a8d1-232beb82271a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1309, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001309__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1310-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1310, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1311, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1632", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1311", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1231a76-e466-49a9-8a72-bf728d764b89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 113940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1310, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001310__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1311-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1311, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1312, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1633", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1312", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07989aae-ba26-4b38-a30f-d7fc185351ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1311, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001311__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1312-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1312, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1313, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1634", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1313", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb010ad8-70ad-466b-880d-7517aca0b206", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1312, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001312__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1313-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1313, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "113984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1314, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1635", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1314", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "882b1889-a343-4571-b615-764f011aa056", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1313, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001313__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1314-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1314, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1315, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1636", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1315", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f98f6c33-1db0-4b2f-9552-330f510ccc91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1314, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001314__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1315-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1315, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1316, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1637", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1316", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d099978-84ed-4c78-8181-6f5b42fedd20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1315, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001315__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1316-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1316, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1317, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1638", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1317", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b14b823-c1de-487e-914a-510b1c5aebc2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1316, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001316__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1317-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1317, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1318, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1639", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1318", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b027bbf-a56d-4383-9d21-3d3ed3448313", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1317, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001317__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1318-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1318, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1319, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1640", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1319", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1652cca6-b7df-4a9d-8e60-2137e90e9c99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1318, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001318__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1319-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1319, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1320, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1641", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1320", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5945f0d2-e4f7-447f-87da-a88cc5cf285b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1319, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001319__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1320-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1320, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1321, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1642", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1321", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b513fbd-57e3-4383-86ab-0ab6fa6486fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1320, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001320__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1321-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1321, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1322, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1643", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1322", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb09be00-0bdc-44a5-855d-155c1c1af27a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1321, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001321__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1322-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1322, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1323, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1644", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1323", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ba635ab-7840-438c-b1f0-56c37d8306ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 114986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1322, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001322__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1323-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1323, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1324, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1645", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1324", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f16e73d-8135-448c-b95f-7297fb82d1db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1323, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001323__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1324-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1324, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "114937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1325, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1646", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1325", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c2bf67c-41f5-4380-b508-620cef80aa47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1324, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001324__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1325-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1325, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1326, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1647", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1326", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75f3c772-43a8-4ea5-ba03-061c8c50fcf6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1325, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001325__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1326-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1326, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1327, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1648", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1327", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9aa72f04-f2b9-46b9-b4f7-ce5637660d2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1326, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001326__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1327-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1327, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1328, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1649", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1328", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ad6f930-aea8-49f3-b212-91c19a372131", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1327, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001327__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1328-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1328, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1329, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1650", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1329", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e87236ce-eccc-4906-9072-09c07e3fcda8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1328, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001328__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1329-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1329, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1330, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1651", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1330", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04989ae6-a1f7-4399-adfe-0faa7903783e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1329, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001329__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1330-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1330, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1331, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1652", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1331", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0042224e-40cd-44be-93c0-3e40e50c4571", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1330, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001330__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1331-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1331, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1332, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1653", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1332", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "002f7363-82fc-4629-bb7a-94d53fb7905f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1331, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001331__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1332-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1332, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1333, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1654", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1333", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "272dacac-5841-4a02-8fb2-d48c13b2a4b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1332, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001332__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1333-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1333, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1334, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1655", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1334", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e920ce9e-21fe-4d4d-a79d-4ce3ce052b2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 115940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1333, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001333__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1334-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1334, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1335, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1656", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1335", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f0de870-8caf-4ace-9a4f-345e762b2844", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1334, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001334__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1335-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1335, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1336, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1657", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1336", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65149c66-dd79-48ed-a2e4-9a27bd01c82c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1335, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001335__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1336-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1336, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "115968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1337, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1658", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1337", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f4560db-a8b5-4914-9b17-10250e3112d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1336, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001336__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1337-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1337, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1338, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1659", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1338", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4dcd3887-2727-442d-abba-187760cb4a75", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1337, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001337__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1338-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1338, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1339, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1660", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1339", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52c3d62b-948a-4ff8-ad0b-06f345b42bd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1338, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001338__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1339-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1339, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1340, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1661", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1340", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2527fe5b-1022-45d5-be29-4021c1a4a8e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1339, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001339__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1340-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1340, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1341, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1662", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1341", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19d7196e-71f1-4a69-bb32-2689fa143554", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1340, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001340__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1341-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1341, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1342, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1663", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1342", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd3cdc58-f1ef-4456-b3ac-6889c9e1e6e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1341, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001341__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1342-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1342, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1343, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1664", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1343", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10f52c12-dec1-45e3-be9a-f8bf760e2918", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1342, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001342__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1343-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1343, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1344, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1665", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1344", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32b2863a-9420-4610-bef9-9fafc5664bb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1343, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001343__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1344-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1344, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1345, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1666", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1345", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4d631fb-1bd9-46e8-889e-856018588135", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1344, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001344__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1345-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1345, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1346, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1667", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1346", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0d21682-082b-402c-ac92-5260a2c0d32e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 116971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1345, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001345__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1346-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1346, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1347, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1668", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1347", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79e5c01b-8ecd-4aa4-8bf1-d678af43c798", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1346, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001346__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1347-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1347, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "116937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1348, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1669", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1348", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2bd21a98-c6e0-4f9e-aa73-f7283acbf83c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1347, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001347__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1348-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1348, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1349, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1670", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1349", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5d49ac4-e8c7-4878-aa43-826286c301a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1348, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001348__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1349-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1349, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1350, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1671", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1350", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b16fa120-26bb-4b07-87cb-33efa8ac6af5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1349, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001349__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1350-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1350, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1351, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1672", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1351", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02abaddf-2805-47a0-a0e2-78bf9944e928", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1350, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001350__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1351-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1351, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1352, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1673", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1352", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b5553dc-9f19-40ea-800e-ed5b43b2871b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1351, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001351__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1352-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1352, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1353, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1674", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1353", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6942afea-db94-48ce-b1aa-08dbf97af652", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1352, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001352__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1353-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1353, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1354, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1675", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1354", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "849f61f6-344a-424b-b0b6-8ee91c865532", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1353, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001353__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1354-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1354, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1355, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1676", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1355", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c088a609-efbe-43d0-8df5-d561082899eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1354, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001354__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1355-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1355, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1356, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1677", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1356", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90b12fcc-b769-4231-8aae-3700b86ba25a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1355, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001355__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1356-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1356, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1357, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1678", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1357", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e50ed49-8c3d-4a0b-b82f-d63f3e8b7ffa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 117924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1356, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001356__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1357-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1357, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1358, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1679", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1358", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0180a22-9ff9-4023-850c-2d3e4faa83a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1357, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001357__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1358-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1358, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1359, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1680", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1359", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c3cb36f-ce11-4786-942e-208076469366", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118111.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1358, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001358__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1359-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1359, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "117968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1360, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1681", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1360", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e35fde96-3ebb-44e5-970f-35b3a8cf2044", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1359, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001359__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1360-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1360, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1361, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1682", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1361", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6a8ad09-ba1f-4c3a-a2bc-b6fa6cb550b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1360, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001360__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1361-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1361, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1362, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1683", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1362", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ecb14ee-b269-4997-9e79-0820a3c23102", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1361, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001361__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1362-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1362, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1363, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1684", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1363", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a87f799-71f7-4af0-a5a5-19e4872b0fb3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118455.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1362, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001362__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1363-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1363, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1364, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1685", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1364", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "012897d1-96a1-43bd-b51b-dcecf374aae1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1363, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001363__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1364-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1364, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1365, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1686", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1365", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d3c2f51-d7af-4880-bde0-46334d6e3756", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1364, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001364__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1365-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1365, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1366, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1687", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1366", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d58a024-5e06-4bf6-bc22-f18741c55157", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1365, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001365__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1366-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1366, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1367, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1688", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1367", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5396066d-28e5-4fb0-bab3-d1ef9ece53b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1366, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001366__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1367-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1367, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1368, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1689", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1368", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb2cd34c-7a6a-4638-9933-0a1d0de48115", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118893.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1367, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001367__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1368-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1368, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1369, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1690", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1369", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea7ee3ba-2dfa-4dee-aba6-1daff85d0c5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 118971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1368, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001368__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1369-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1369, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1370, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1691", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1370", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3aa337c-eb7c-4aaa-8627-126f988d717a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1369, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001369__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1370-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1370, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "118921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1371, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1692", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1371", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6dd31b21-8ba1-4c3f-a342-2f1c49ec1213", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1370, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001370__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1371-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1371, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1372, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1693", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1372", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9103e81d-c07b-4213-840e-01cacd74cf04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119236.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1371, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001371__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1372-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1372, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1373, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1694", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1373", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e62e3e9-4c7a-4749-bbab-822ad7b5b8bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1372, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001372__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1373-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1373, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1374, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1695", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1374", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cfb9365-a7ab-4ae8-abdb-869041116c1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1373, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001373__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1374-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1374, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1375, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1696", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1375", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47c50255-b837-4d27-a263-cc7343f1a25c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1374, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001374__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1375-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1375, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1376, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1697", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1376", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77dfd60f-8272-44e0-b44d-cc8d195d5cbd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1375, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001375__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1376-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1376, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1377, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1698", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1377", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "922343e3-d6b8-4095-97d8-0f4f020bc606", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1376, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001376__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1377-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1377, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1378, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1699", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1378", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f062d7f-fcab-45c5-979b-b221520b2b31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1377, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001377__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1378-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1378, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1379, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1700", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1379", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92a813a3-8059-4215-9be9-de0a58179e76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1378, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001378__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1379-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1379, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1380, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1701", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1380", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2872a09d-c313-48d6-b325-d9e954d7db56", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 119924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1379, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001379__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1380-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1380, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1381, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1702", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1381", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d853c76a-8bd0-4d77-8537-364658532640", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120018.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1380, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001380__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1381-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1381, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1382, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1703", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1382", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e4fe226-69a6-4840-8cf3-ba18873dd4b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1381, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001381__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1382-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1382, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "119968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1383, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1704", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1383", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c46d88c5-28cf-4d98-9f97-65dbc91f0407", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1382, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001382__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1383-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1383, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1384, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1705", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1384", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee083e7f-9b18-4601-aef9-498863a79243", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1383, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001383__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1384-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1384, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1385, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1706", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1385", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "090d4234-12d1-4de3-9459-fa0c4f052e05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1384, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001384__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1385-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1385, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1386, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1707", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1386", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81bb9e14-5a63-49a5-9d9a-ffbf91418a2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1385, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001385__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1386-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1386, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1387, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1708", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1387", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e05b15bf-a80c-4432-8e2a-073f44cae198", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1386, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001386__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1387-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1387, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1388, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1709", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1388", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "049196ab-c61a-41df-8147-b757d39195f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1387, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001387__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1388-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1388, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1389, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1710", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1389", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60234ea5-36a4-4692-8e74-93d4277259c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1388, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001388__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1389-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1389, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1390, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1711", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1390", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae86c66d-78b1-489b-95b8-46843e853db2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120799.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1389, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001389__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1390-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1390, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1391, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1712", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1391", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3a75821-f720-4490-be84-a23387f23503", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1390, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001390__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1391-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1391, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1392, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1713", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1392", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1544df9-b223-4ee4-9d59-f3ada74b1b1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 120971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1391, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001391__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1392-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1392, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1393, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1714", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1393", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2bb7d55d-4bfc-4246-9956-c2fa8ad65601", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1392, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001392__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1393-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1393, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "120921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1394, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1715", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1394", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebc1c8db-4fa9-4e44-a5a5-3d883e4ccda2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1393, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001393__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1394-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1394, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1395, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1716", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1395", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f70e02e-416c-44c5-9147-4446ad70f87e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1394, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001394__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1395-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1395, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1396, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1717", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1396", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c23ba177-d948-4fec-a383-36d067b54748", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1395, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001395__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1396-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1396, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1397, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1718", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1397", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea501895-5067-4e23-99bf-e247dfc9936c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1396, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001396__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1397-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1397, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1398, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1719", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1398", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b81c812b-4a0d-4e8f-9d21-fdb678d80540", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1397, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001397__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1398-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1398, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1399, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1720", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1399", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95e838ed-5d8d-4572-a358-86287d7215fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121580.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1398, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001398__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1399-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1399, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1400, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1721", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1400", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be355ffa-943e-423d-b350-0d6a0f7235bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1399, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001399__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1400-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1400, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1401, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1722", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1401", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91e68136-844c-4d28-9b6c-6a2c0ff80207", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1400, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001400__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1401-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1401, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1402, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1723", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1402", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b19a717d-21ed-4e25-be7b-40f61bc22791", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1401, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001401__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1402-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1402, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1403, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1724", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1403", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef6cef31-2fc6-4a69-8516-355601bf9103", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 121924.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1402, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001402__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1403-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1403, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1404, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1725", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1404", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ef20672-38ab-4461-9071-63d3c06b28be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1403, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001403__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1404-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1404, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1405, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1726", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1405", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cd734d8-c5fa-44ee-bbb9-13813e3b8e80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1404, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001404__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1405-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1405, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "121953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1406, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1727", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1406", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f9e6148-74ff-47b8-9d88-56a5342c1d51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1405, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001405__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1406-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1406, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1407, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1728", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1407", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1880969d-85af-45ae-951b-a761d13b2fa4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1406, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001406__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1407-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1407, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1408, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1729", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1408", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d63313d9-b4bf-458d-ab40-e17e160582c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122361.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1407, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001407__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1408-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1408, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1409, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1730", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1409", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68ada478-6613-4d4e-ba79-0df7a12d500f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1408, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001408__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1409-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1409, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1410, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1731", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1410", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5049473b-dd0a-473a-ad39-cba28e3e5ccb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1409, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001409__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1410-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1410, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1411, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1732", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1411", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69caa17e-ffa9-4ff7-8627-c585e790bd86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1410, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001410__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1411-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1411, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1412, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1733", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1412", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78a1aa9f-4b57-4aa5-aaff-8fa9c18485aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122705.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1411, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001411__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1412-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1412, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1413, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1734", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1413", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbc72bde-4869-4b43-a785-f0af5abd1e9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1412, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001412__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1413-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1413, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1414, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1735", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1414", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a613649-87e1-40f9-848a-58caaa68ca43", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1413, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001413__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1414-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1414, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1415, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1736", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1415", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d908430b-9edc-4110-9082-0f87a3e9c19e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 122955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1414, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001414__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1415-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1415, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1416, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1737", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1416", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2ec7071-1e8a-4893-a73d-968feea45b5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1415, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001415__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1416-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1416, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "122921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1417, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1738", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1417", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e940e2da-2921-440c-84c8-8ae471c76559", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123143.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1416, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001416__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1417-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1417, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1418, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1739", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1418", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8793ada-2eaa-4dda-b4d2-2e69ff7cb495", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1417, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001417__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1418-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1418, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1419, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1740", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1419", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4a413d7-37a0-4957-a636-0f191e96e0c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1418, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001418__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1419-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1419, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1420, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1741", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1420", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54564a13-b806-4727-8466-9d292cd47f6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1419, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001419__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1420-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1420, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1421, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1742", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1421", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0e534c4-9b57-4300-b6f4-6599967445fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123486.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1420, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001420__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1421-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1421, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1422, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1743", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1422", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94d74276-941f-45ca-a326-07a101a73d40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1421, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001421__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1422-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1422, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1423, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1744", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1423", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "530a6618-b5c1-4edc-949e-929c8deef8cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1422, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001422__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1423-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1423, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1424, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1745", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1424", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01cbc2fb-c5f4-4e59-ac34-383b65a3faca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1423, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001423__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1424-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1424, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1425, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1746", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1425", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a077183-62ae-466f-b827-43204fe0ea84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1424, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001424__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1425-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1425, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1426, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1747", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1426", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0361e7a3-7134-4f0a-958d-be70b806527c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 123908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1425, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001425__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1426-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1426, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1427, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1748", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1427", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1b14202-28b5-408c-8909-fb593d3a13dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1426, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001426__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1427-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1427, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1428, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1749", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1428", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c9dc5d53-5ba3-4b5c-aaca-43722db7140a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1427, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001427__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1428-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1428, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "123953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1429, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1750", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1429", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d46603c3-6849-477e-a20a-195568c4235a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1428, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001428__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1429-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1429, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1430, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1751", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1430", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c913b3ce-23de-424a-b8a7-12dc94aef269", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124268.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1429, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001429__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1430-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1430, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1431, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1752", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1431", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfaebe85-9f80-48f3-bfd8-5287f0d97871", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1430, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001430__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1431-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1431, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1432, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1753", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1432", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b31c7112-0d85-4338-bac6-e10ad646044d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1431, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001431__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1432-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1432, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1433, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1754", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1433", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32bf0b80-bfa4-457b-8bb0-3c42a77cc30d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1432, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001432__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1433-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1433, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1434, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1755", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1434", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79d9063e-b4cd-4f00-82f4-f4619ddda239", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1433, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001433__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1434-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1434, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1435, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1756", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1435", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ba8b115-f9e9-450b-9e72-984b856693b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1434, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001434__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1435-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1435, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1436, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1757", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1436", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78738dc5-f134-4b3b-a9cd-ab821ec7dfa6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1435, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001435__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1436-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1436, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1437, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1758", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1437", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d296bdf-809e-41f2-9acd-d9362c9d30b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1436, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001436__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1437-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1437, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1438, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1759", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1438", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8bf0c12-ca93-43a5-949e-71ed5d7ba645", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1437, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001437__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1438-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1438, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1439, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1760", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1439", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16581242-832b-4500-83f8-5dbf74b7f4c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125049.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1438, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001438__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1439-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1439, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "124906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1440, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1761", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1440", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5a71100-c063-45a5-a362-2ab2a95f17a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1439, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001439__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1440-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1440, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1441, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1762", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1441", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab8945ab-740a-4c9c-b073-4c88edc2dd3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1440, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001440__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1441-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1441, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1442, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1763", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1442", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb6a5ea9-603b-4609-914f-8ffb865ae939", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1441, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001441__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1442-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1442, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1443, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1764", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1443", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "112818a8-557c-47cb-bd36-4b6dd9225293", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1442, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001442__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1443-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1443, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1444, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1765", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1444", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b61a2d8-237f-4f2f-8d83-d581f05d0ae4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1443, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001443__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1444-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1444, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1445, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1766", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1445", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b84ccf85-8bbf-4e5e-91c3-c30895f4a77a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1444, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001444__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1445-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1445, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1446, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1767", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1446", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91ebd7c6-fb74-4b14-9d7a-394d7009b70d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1445, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001445__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1446-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1446, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1447, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1768", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1447", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f180f8d9-a395-488d-9b52-cb7decd94730", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1446, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001446__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1447-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1447, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1448, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1769", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1448", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f265eda3-e9f0-4e6a-9500-296f6ed81be1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125830.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1447, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001447__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1448-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1448, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1449, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1770", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1449", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "590ce682-eb58-482e-8553-a7375d23533d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 125908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1448, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001448__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1449-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1449, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1450, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1771", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1450", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3382c8e3-06b8-4d3e-a161-7f37f9e39b71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1449, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001449__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1450-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1450, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1451, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1772", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1451", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0547ba33-b439-4807-a65c-46eba7ad1e66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1450, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001450__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1451-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1451, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "125953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1452, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1773", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1452", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9399c24-b725-4fbe-bc5b-e0a9d55495d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1451, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001451__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1452-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1452, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1453, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1774", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1453", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "039038d7-4763-4b66-92ab-35d47f818464", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1452, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001452__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1453-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1453, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1454, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1775", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1454", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da26a30b-0269-40f5-8e4f-a314af8fa002", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1453, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001453__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1454-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1454, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1455, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1776", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1455", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1825fac-1fa1-4ade-89ae-0dbb6c542818", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1454, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001454__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1455-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1455, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1456, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1777", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1456", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36a1472f-2369-41f6-aa60-cd1e977a1ea4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1455, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001455__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1456-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1456, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1457, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1778", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1457", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43dec350-73d6-4383-b31e-58594f9ae9f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126611.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1456, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001456__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1457-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1457, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1458, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1779", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1458", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27986c20-3b30-4f27-8564-4bbf6f9f10f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1457, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001457__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1458-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1458, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1459, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1780", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1459", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "242eccc8-99d8-44be-9e42-6b7fe66dae6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1458, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001458__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1459-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1459, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1460, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1781", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1460", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "623f482c-5017-4eaa-8073-dd75d07b7989", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1459, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001459__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1460-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1460, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1461, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1782", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1461", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d96c4dbf-b99a-48cf-b0ae-ca54a8dc7cff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 126955.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1460, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001460__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1461-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1461, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1462, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1783", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1462", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32c738a4-8d65-4c40-940c-a49f5a58b139", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1461, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001461__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1462-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1462, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1463, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1784", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1463", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c68434e3-6a29-4da5-b1f7-bb347f0ee9e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1462, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001462__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1463-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1463, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "126984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1464, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1785", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1464", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9305ae76-1e5e-4aad-9a44-d58f5063317d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1463, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001463__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1464-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1464, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1465, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1786", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1465", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d1eaec2-4cc8-4b41-aa89-29b346dde66b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1464, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001464__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1465-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1465, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1466, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1787", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1466", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "111edd2c-1c36-4c4f-9273-e4719e5c749a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127393.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1465, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001465__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1466-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1466, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1467, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1788", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1467", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "573e1a5d-3dff-41e3-9802-694393917b50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1466, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001466__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1467-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1467, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1468, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1789", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1468", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e469c4e-3985-4eb6-831e-48431d6579ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1467, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001467__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1468-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1468, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1469, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1790", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1469", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb496076-e038-4f01-bd7b-103e152572c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1468, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001468__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1469-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1469, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1470, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1791", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1470", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9f59599-51f4-41b4-ad2b-f591d908af06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127736.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1469, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001469__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1470-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1470, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1471, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1792", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1471", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "541ee1e3-1d4a-4227-a390-3df1566f9a4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1470, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001470__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1471-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1471, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1472, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1793", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1472", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f3711f1-9002-4d84-beef-76434564e4d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1471, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001471__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1472-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1472, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1473, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1794", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1473", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d08824a2-2170-4b4e-9b46-32676a8a3762", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 127986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1472, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001472__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1473-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1473, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1474, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1795", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1474", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a2f8365-195e-493d-beb1-efbb564792c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1473, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001473__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1474-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1474, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "127953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1475, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1796", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1475", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "505c7578-9cb9-4c8a-9281-6f4933be3ee6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128174.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1474, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001474__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1475-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1475, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1476, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1797", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1476", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c665c2b1-4b9c-4c46-894a-e3807879b9ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1475, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001475__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1476-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1476, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1477, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1798", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1477", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1818477-6fe9-4d04-bf89-bc575ab7b5c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1476, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001476__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1477-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1477, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1478, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1799", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1478", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4118e49-496e-469f-b013-8823085ff55f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1477, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001477__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1478-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1478, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1479, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1800", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1479", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bcaa49f-7b7c-4128-a9a5-05dac8c830ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128518.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1478, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001478__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1479-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1479, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1480, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1801", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1480", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcda0f1e-7d39-4c6d-b5aa-8058d7334590", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1479, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001479__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1480-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1480, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1481, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1802", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1481", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92788089-445c-4a83-b4ee-0808f7c1031b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1480, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001480__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1481-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1481, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1482, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1803", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1482", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad56a5b0-11e5-4f6c-b18d-9a3c18a09cd4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1481, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001481__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1482-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1482, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1483, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1804", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1483", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ffa7f049-7234-4c86-b9ac-86c6964cf179", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1482, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001482__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1483-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1483, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1484, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1805", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1484", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e9effd7-6a72-4137-b3ec-2b952db0ba08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 128940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1483, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001483__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1484-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1484, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1485, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1806", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1485", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "834aff6d-6ac0-414d-b8d5-e870c0c8f9d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1484, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001484__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1485-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1485, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1486, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1807", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1486", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6477b37c-a83d-4e41-8a2c-8917230cc0b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1485, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001485__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1486-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1486, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "128984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1487, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1808", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1487", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f408eb4-3ca8-40c6-9795-3938e8ce604f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129205.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1486, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001486__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1487-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1487, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1488, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1809", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1488", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2bb6b28b-1c89-43af-a1d0-7baec5903b30", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129299.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1487, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001487__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1488-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1488, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1489, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1810", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1489", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e2aaa1b-b199-42bc-bd17-55a2b2c20b95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1488, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001488__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1489-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1489, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1490, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1811", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1490", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e85f43db-4d40-404f-804c-3b8229144f4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1489, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001489__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1490-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1490, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1491, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1812", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1491", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fa0859d-a6be-4f11-9e25-fb10a85ef26f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129549.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1490, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001490__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1491-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1491, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1492, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1813", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1492", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69b227db-730e-4c41-9a94-8b8d538a2af4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129643.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1491, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001491__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1492-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1492, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1493, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1814", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1493", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a13ec86-63a7-4b28-baaa-0c607fd2bfd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1492, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001492__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1493-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1493, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1494, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1815", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1494", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ca0cf8c-11a3-474d-ac32-01806391973e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1493, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001493__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1494-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1494, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1495, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1816", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1495", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f08df18c-1d7b-4b31-b692-5ab566fab0b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1494, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001494__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1495-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1495, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1496, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1817", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1496", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ec65c9b-59f2-4326-8413-9fb14a4fdf08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 129986.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1495, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001495__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1496-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1496, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1497, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1818", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1497", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6966b8be-a5e6-4b8e-acb4-b02b8717d699", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130080.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1496, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001496__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1497-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1497, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "129937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1498, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1819", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1498", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64a78911-8b44-42c2-a4b5-bee66fea82f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1497, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001497__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1498-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1498, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1499, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1820", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1499", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98b57568-d6c8-4855-8bf9-cefc2a0c315b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1498, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001498__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1499-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1499, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1500, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1821", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1500", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63f58a43-386d-4104-8b88-d9ceef75fd44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130330.61999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1499, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001499__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1500-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1500, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1501, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1822", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1501", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92e3e220-cf63-47da-8dfa-d62346095181", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130424.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1500, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001500__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1501-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1501, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1502, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1823", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1502", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30f98a8c-8f67-4691-8cf1-d3cfde41d068", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1501, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001501__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1502-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1502, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1503, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1824", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1503", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37a0217f-0c7b-4af6-a1f1-05ec9a06c26e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1502, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001502__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1503-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1503, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1504, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1825", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1504", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a3ddb3c-6d5e-47e1-b5a2-9c331153f377", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130674.36999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1503, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001503__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1504-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1504, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1505, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1826", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1505", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b495bb0-ab83-43b4-b1ba-6603f1c9b29b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130768.11999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1504, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001504__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1505-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1505, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1506, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1827", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1506", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0dbdc167-8ac6-4411-8aa6-595c10d43b4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130861.86999511719, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1505, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001505__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1506-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1506, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1507, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1828", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1507", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11898f1c-1764-449b-a4e8-5a8c3cf77190", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 130940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1506, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001506__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1507-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1507, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1508, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1829", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1508", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0881d7d-996c-4029-b177-46631f7f40f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1507, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001507__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1508-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1508, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1509, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1830", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1509", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b330ae5f-8f26-4555-8bc7-4cbfab659069", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1508, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001508__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1509-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1509, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "130984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1510, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1831", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1510", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2a3e0f7-262c-4395-b5d4-08a68b308980", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1509, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001509__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1510-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1510, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1511, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1832", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1511", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b7ff8b13-3217-4f49-bbfd-cf9b305488d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1510, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001510__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1511-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1511, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1512, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1833", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1512", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23bdbe7f-3bb2-4a21-92b5-8a1d5d9929bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1511, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001511__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1512-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1512, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1513, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1834", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1513", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38bd57a7-4f22-436d-8565-3fecea198523", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1512, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001512__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1513-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1513, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1514, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1835", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1514", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "722bfcb6-3353-4a43-9add-7eb8b95a384b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1513, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001513__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1514-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1514, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1515, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1836", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1515", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90ddb17b-2c97-4ee5-86e0-911e3bd22662", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1514, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001514__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1515-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1515, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1516, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1837", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1516", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8767fef6-4ea5-4245-baeb-81453fd5164f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1515, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001515__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1516-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1516, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1517, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1838", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1517", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "374b9dda-e799-49bf-82a0-e479c4137f48", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1516, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001516__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1517-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1517, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1518, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1839", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1518", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07063cc1-4505-4ab0-a173-63e6ea9cc651", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1517, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001517__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1518-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1518, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1519, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1840", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1519", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cd375b0-be50-41a4-956f-65e916ff3851", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 131986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1518, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001518__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1519-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1519, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1520, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1841", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1520", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e232365-a787-40a0-8aad-b6f2c0845fe3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1519, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001519__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1520-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1520, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "131937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1521, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1842", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1521", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f60755a-1154-4f6f-ac53-73b563b8df17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1520, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001520__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1521-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1521, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1522, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1843", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1522", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3d1d066-f342-44cc-93a9-bb48dae35489", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1521, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001521__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1522-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1522, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1523, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1844", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1523", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ac1763a-38e2-4ecd-8bbd-dfead1266d4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1522, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001522__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1523-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1523, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1524, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1845", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1524", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea20555d-9f69-4df3-97d4-7a0926ca16b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1523, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001523__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1524-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1524, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1525, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1846", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1525", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4992ac5-f7f3-44ea-880b-c00bcee75410", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1524, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001524__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1525-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1525, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1526, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1847", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1526", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98e52fa1-9d01-4fed-afc1-85773e15875e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1525, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001525__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1526-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1526, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1527, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1848", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1527", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea574e3a-ce70-4565-adc1-629d00d05d2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1526, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001526__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1527-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1527, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1528, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1849", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1528", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00413544-f075-4634-8b74-60a7a83c20c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1527, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001527__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1528-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1528, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1529, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1850", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1529", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9153829-eea1-416b-bfb9-82f04700242a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1528, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001528__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1529-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1529, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1530, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1851", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1530", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63850d01-47fb-4184-8b54-73ff50b16574", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 132940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1529, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001529__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1530-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1530, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1531, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1852", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1531", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a41bf27d-74fc-48aa-9c87-22ef3f01bd4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1530, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001530__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1531-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1531, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1532, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1853", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1532", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a0901ea-bcc7-4858-b2a5-0fc10f230ec1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1531, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001531__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1532-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1532, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "132968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1533, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1854", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1533", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80497c05-39f7-4b3d-9920-02003f1d3a20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1532, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001532__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1533-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1533, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1534, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1855", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1534", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f84ef916-7f0a-484a-bc7b-cdcf92a9196e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1533, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001533__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1534-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1534, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1535, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1856", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1535", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c1ec2ea-2146-4ec2-8d45-54471c4ce3ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1534, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001534__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1535-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1535, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1536, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1857", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1536", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b3df009-5023-4ed2-8106-41124636d9b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1535, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001535__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1536-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1536, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1537, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1858", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1537", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca2f3bf4-fcb8-42e9-9728-ccdfde81dca4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1536, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001536__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1537-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1537, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1538, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1859", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1538", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e2550d8-fe16-4efb-bfd1-1a3a28605b4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1537, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001537__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1538-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1538, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1539, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1860", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1539", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64d0f63f-ff58-45c3-9301-4d1d37c97290", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1538, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001538__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1539-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1539, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1540, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1861", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1540", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b141a2d0-1a19-4998-a383-67fbea88e4b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1539, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001539__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1540-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1540, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1541, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1862", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1541", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4eb838f-6028-4840-8544-cff038fcd9f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1540, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001540__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1541-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1541, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1542, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1863", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1542", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17d0f5d3-525d-423e-a702-26207cdf9f4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 133971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1541, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001541__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1542-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1542, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1543, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1864", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1543", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3a57535-f08f-4a1d-93a0-f77047702c5c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1542, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001542__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1543-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1543, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "133937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1544, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1865", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1544", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "513573f9-6fd8-4893-91ad-c1a123059a5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1543, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001543__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1544-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1544, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1545, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:20:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1866", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1545", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e684db2c-47b6-421a-9118-3ee4816caf47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1544, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001544__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1545-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1545, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1546, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1867", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1546", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9422fc4c-6b4a-46f4-824d-04144f25702c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1545, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001545__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1546-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1546, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1547, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1868", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1547", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1677b69-22d8-43de-bed4-6772c98bd67b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1546, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001546__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1547-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1547, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1548, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1869", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1548", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ac117d8-2353-4a77-805f-568b7cda35c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1547, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001547__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1548-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1548, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1549, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1870", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1549", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68ed4ef1-60eb-44c4-80bf-61b2631fff68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1548, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001548__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1549-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1549, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1550, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1871", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1550", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84266bca-719e-4621-823d-3dec58f532dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1549, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001549__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1550-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1550, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1551, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1872", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1551", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a194d64-2bc6-423e-abd2-fad8765599e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1550, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001550__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1551-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1551, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1552, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1873", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1552", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "039e5659-7fc2-4272-8d56-f181a505b057", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1551, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001551__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1552-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1552, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1553, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1874", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1553", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "770f6bec-d00c-4066-bded-52d018dea0f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 134940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1552, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001552__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1553-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1553, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1554, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1875", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1554", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6881e0a9-74cc-413d-98d7-668fc28d8798", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1553, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001553__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1554-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1554, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1555, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1876", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1555", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03b5659a-88bc-4140-8385-3052e5c3cea6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1554, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001554__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1555-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1555, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "134968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1556, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1877", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1556", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e2bf60a-0e60-4d7a-a0c1-ffb06dc2ef13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1555, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001555__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1556-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1556, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1557, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1878", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1557", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06a6b971-d82d-4639-bf55-c9d9b7a9d61c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1556, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001556__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1557-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1557, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1558, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1879", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1558", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa4ba580-4ab6-4aa9-a6e2-eb6670c211dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1557, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001557__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1558-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1558, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1559, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1880", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1559", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "425f4975-b460-411d-aa31-29b86f3d68d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1558, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001558__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1559-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1559, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1560, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1881", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1560", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d6fa903-2794-4eb6-8a9c-6e2180186ad6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1559, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001559__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1560-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1560, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1561, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1882", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1561", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90ac8ad7-06b4-46ad-8639-f8421c8f059a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1560, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001560__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1561-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1561, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1562, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1883", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1562", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8347785f-9bc2-4679-95f6-5c3120feb549", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1561, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001561__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1562-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1562, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1563, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1884", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1563", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecc5c3ac-0d31-49b9-8a88-2c2896a716ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1562, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001562__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1563-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1563, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1564, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1885", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1564", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9db41d23-c4cf-4621-840e-2a54ffee3ee1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1563, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001563__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1564-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1564, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1565, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1886", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1565", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf83d26f-d9ee-48cb-a098-23eb939bdca6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 135971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1564, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001564__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1565-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1565, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1566, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1887", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1566", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fd4e076-cfdf-449a-9bd9-9999468f114b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1565, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001565__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1566-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1566, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "135921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1567, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1888", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1567", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88126f54-4e55-4a40-bcd9-b3f9986e1d24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1566, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001566__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1567-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1567, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1568, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1889", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1568", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6fa6c7e-7d91-4b3e-aa41-1bd7a3de9785", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1567, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001567__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1568-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1568, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1569, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1890", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1569", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65def028-bb17-48b0-8fd5-8307713d4651", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1568, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001568__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1569-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1569, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1570, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1891", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1570", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55935e81-b63f-4bb9-b812-0120e5cd233e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1569, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001569__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1570-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1570, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1571, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1892", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1571", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46980364-3b1b-4ab1-9585-06b45f7a2e0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1570, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001570__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1571-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1571, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1572, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1893", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1572", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6decbfb-00b0-44f2-8828-19f71ce1dd3c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1571, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001571__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1572-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1572, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1573, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1894", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1573", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85ebb5ec-7017-4d69-aa54-84e204c68c90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1572, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001572__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1573-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1573, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1574, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1895", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1574", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c87be43-50d8-4e52-820f-17c9b0e4c591", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1573, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001573__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1574-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1574, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1575, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1896", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1575", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76e116be-097b-4334-9c8a-8454057cfe38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1574, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001574__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1575-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1575, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1576, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1897", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1576", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da835c9a-fc7c-4325-88a6-25134e127e95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 136924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1575, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001575__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1576-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1576, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1577, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1898", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1577", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e889d03-33fc-4c9f-b8b3-92eca76d2c28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1576, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001576__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1577-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1577, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1578, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1899", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1578", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e632a967-6a1d-4760-9cb3-35d6718b571a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1577, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001577__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1578-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1578, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "136968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1579, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1900", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1579", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02156e87-ae98-4a03-9948-020c8fcba986", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1578, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001578__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1579-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1579, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1580, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1901", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1580", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec44c97e-328c-4eec-8acb-393d5aeda9f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1579, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001579__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1580-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1580, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1581, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1902", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1581", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37b19df6-087f-450e-9983-a7bbf234d545", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1580, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001580__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1581-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1581, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1582, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1903", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1582", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f155c85-618e-4663-84ba-e755059402e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1581, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001581__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1582-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1582, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1583, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1904", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1583", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4975e979-6ccd-44b8-9227-bd4aa688f0fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1582, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001582__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1583-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1583, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1584, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1905", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1584", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c69e919-a5e5-4c6a-844d-4321783f08b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1583, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001583__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1584-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1584, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1585, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1906", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1585", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bed05cfa-bd9b-4923-8431-a3b219309ed7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1584, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001584__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1585-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1585, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1586, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1907", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1586", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98f3779f-2c2b-4d43-8b4f-cc56d48dde05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1585, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001585__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1586-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1586, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1587, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1908", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1587", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "930c645b-9f82-4509-bc30-fb81fcc7d37e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1586, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001586__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1587-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1587, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1588, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1909", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1588", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e7c6436-631f-43de-9180-ea8471e17bef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 137971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1587, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001587__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1588-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1588, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1589, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1910", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1589", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "236bcda7-c2f1-4a16-a5b5-2aaf2d829aba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1588, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001588__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1589-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1589, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "137921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1590, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1911", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1590", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6347be90-9280-4e82-871b-a316412575b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1589, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001589__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1590-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1590, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1591, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1912", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1591", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02442663-cc3a-4939-a8af-ed71e997ccff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1590, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001590__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1591-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1591, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1592, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1913", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1592", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75342972-a37f-4127-aa37-10976cfafefc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1591, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001591__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1592-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1592, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1593, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1914", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1593", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e424d87-9148-481d-b581-1b5f75de801a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1592, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001592__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1593-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1593, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1594, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1915", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1594", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2eb338fe-6c63-42e2-bdc5-c49411f5f24d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1593, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001593__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1594-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1594, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1595, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1916", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1595", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fc18b45-54f5-4ebd-a713-b373c08f3f4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1594, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001594__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1595-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1595, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1596, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1917", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1596", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "790e2850-71ec-422d-8eda-1d11aea7a776", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1595, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001595__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1596-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1596, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1597, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1918", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1597", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "219488dd-c486-4118-9155-16c6da6b21ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1596, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001596__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1597-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1597, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1598, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1919", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1598", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3218508-1034-4c62-9ced-5097df078a36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1597, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001597__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1598-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1598, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1599, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1920", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1599", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a5df3f3-ad89-484e-92d2-8fb70d4fce14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 138924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1598, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001598__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1599-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1599, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1600, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1921", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1600", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e7984ca-6376-416d-a129-497dd218fe45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1599, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001599__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1600-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1600, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1601, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1922", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1601", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ddd72b5-7ca3-4439-9f5c-cda416340d85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1600, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001600__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1601-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1601, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "138968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1602, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1923", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1602", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ca5b0ef-c57e-4890-b043-ca12acc3c380", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1601, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001601__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1602-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1602, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1603, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1924", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1603", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "450fa18d-c5df-4a6f-973e-c5f403a67d3a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1602, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001602__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1603-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1603, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1604, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1925", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1604", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36bff339-bac9-41ff-835d-fbe54f7c1114", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1603, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001603__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1604-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1604, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1605, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1926", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1605", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0b87904-884a-4fd8-9840-57a4fe30e820", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1604, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001604__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1605-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1605, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1606, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1927", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1606", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d30f1a6-b884-46c4-82b5-c7d73992b8bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1605, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001605__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1606-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1606, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1607, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1928", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1607", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd4e1bbe-0337-4ee8-b9db-d099e9970de9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1606, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001606__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1607-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1607, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1608, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1929", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1608", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4aaa7f9-a4c0-4897-bcd1-116b0222d4df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1607, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001607__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1608-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1608, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1609, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1930", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1609", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae3bccb9-d9e5-4286-a3e3-8cac2af9032b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1608, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001608__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1609-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1609, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1610, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1931", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1610", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76ddcb7a-5b32-4828-b93d-d6ed8ba51ca8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1609, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001609__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1610-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1610, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1611, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1932", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1611", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5384473e-f348-4282-a67d-326e1da6f3ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 139955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1610, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001610__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1611-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1611, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1612, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1933", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1612", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaaf0f71-dcc0-4538-a1f3-dae36bdcaeb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1611, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001611__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1612-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1612, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "139921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1613, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1934", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1613", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d9622ce-9b5b-48ce-abcf-a33e89e042ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1612, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001612__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1613-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1613, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1614, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1935", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1614", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d19c6583-72c9-4ef2-bae7-cf118eeed09c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1613, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001613__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1614-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1614, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1615, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1936", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1615", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1570eb1f-bff7-4369-8f9e-ae44f89b082a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1614, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001614__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1615-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1615, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1616, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1937", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1616", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "878d8336-cfc8-44e0-896e-d5e13d398ad3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1615, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001615__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1616-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1616, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1617, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1938", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1617", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e1bc7b8-8841-4218-83e1-00a73187ae57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1616, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001616__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1617-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1617, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1618, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1939", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1618", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5361caa4-7f47-43e7-86d1-fa4f7c7ebb78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1617, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001617__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1618-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1618, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1619, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1940", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1619", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fc22c7a-0c3c-41ef-b2aa-354e2adf2cb5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1618, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001618__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1619-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1619, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1620, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1941", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1620", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a8ee410-be51-4f39-b754-c92a048b8cd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1619, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001619__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1620-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1620, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1621, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1942", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1621", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22ff1d3e-a409-4ee1-898c-a21232f4e967", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1620, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001620__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1621-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1621, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1622, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1943", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1622", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc71f2df-1c97-43cc-9672-ac01bb28d093", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 140924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1621, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001621__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1622-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1622, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1623, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1944", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1623", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74d835e6-715e-4cbe-8799-a8de951959b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1622, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001622__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1623-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1623, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1624, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1945", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1624", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54b8052d-63cb-4dbf-aa55-49e252c30078", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1623, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001623__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1624-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1624, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "140953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1625, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1946", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1625", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a85946ab-8ddf-46db-9ca2-a6e2515da94e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1624, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001624__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1625-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1625, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1626, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1947", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1626", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baf797e4-682e-4be0-a1a7-102310e17514", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1625, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001625__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1626-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1626, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1627, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1948", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1627", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40c636ce-12e4-4df9-98d9-fa34e638109d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1626, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001626__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1627-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1627, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1628, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1949", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1628", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcf2f9ad-bf05-42a9-8535-1dccf1ffa6fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1627, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001627__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1628-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1628, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1629, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1950", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1629", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0900959e-5482-4b3f-871a-2e123fb5100b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1628, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001628__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1629-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1629, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1630, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1951", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1630", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e6ea79f-2375-49d2-b0cc-e940b0329ac3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1629, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001629__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1630-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1630, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1631, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1952", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1631", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81b46b49-3780-4dfb-92cc-f886d862cdf3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1630, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001630__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1631-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1631, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1632, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1953", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1632", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "282bb3a3-74f1-4b6a-9cde-fe9c62f28791", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1631, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001631__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1632-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1632, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1633, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1954", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1633", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a0f7e08-539b-44e7-aa92-344b4dc3013a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1632, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001632__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1633-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1633, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1634, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1955", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1634", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f51131c7-6b9a-4e0c-b8e6-d3fea6d42c64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 141955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1633, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001633__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1634-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1634, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1635, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1956", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1635", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4305eabc-837f-4746-8fe8-6ae85a95f008", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1634, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001634__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1635-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1635, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "141906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1636, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1957", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1636", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f15668c-331d-448b-89c8-9e017c46bee8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1635, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001635__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1636-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1636, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1637, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1958", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1637", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe04da19-57c3-4548-9390-bfca26c36da5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1636, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001636__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1637-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1637, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1638, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1959", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1638", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6811fee3-4197-4d38-880a-46797ef904e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1637, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001637__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1638-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1638, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1639, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1960", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1639", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72777e98-da10-4538-a7f0-262c8c689a8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1638, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001638__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1639-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1639, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1640, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1961", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1640", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b0d6a1d-3424-414f-a502-10b0544f7b9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1639, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001639__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1640-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1640, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1641, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1962", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1641", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f91b8661-f13a-4c4f-9e81-e944038039cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1640, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001640__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1641-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1641, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1642, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1963", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1642", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5624cdbe-06c1-41e6-ad2f-8f0667b63a1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1641, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001641__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1642-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1642, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1643, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1964", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1643", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95e61d82-b9ed-40ff-adee-2138534404a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1642, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001642__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1643-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1643, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1644, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1965", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1644", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f5758cd-492b-49d5-9693-9c4596bcb3de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1643, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001643__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1644-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1644, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1645, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1966", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1645", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1af69f49-7267-4d95-a15f-7886cda6ff94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 142908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1644, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001644__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1645-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1645, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1646, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1967", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1646", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a8d985a-56da-4c09-88ad-b535fe35eba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1645, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001645__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1646-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1646, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1647, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1968", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1647", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d14161f-8987-49c6-8d52-388c0c02c0de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1646, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001646__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1647-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1647, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "142953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1648, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1969", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1648", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b388faf-e116-48e6-aceb-32853737e7a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1647, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001647__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1648-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1648, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1649, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1970", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1649", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5b64ee3-38c6-4f08-86ec-5fd0f872f3ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1648, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001648__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1649-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1649, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1650, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1971", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1650", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "387aec80-b120-4043-a94b-19dda3d04721", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1649, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001649__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1650-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1650, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1651, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1972", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1651", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "268b9195-2c42-4cfe-9501-7c7979b82cc9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1650, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001650__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1651-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1651, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1652, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1973", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1652", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28655829-f230-4f2e-9864-1ee102910ee9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1651, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001651__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1652-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1652, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1653, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1974", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1653", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26a76864-f69b-4528-8b47-aca172e10897", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1652, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001652__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1653-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1653, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1654, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1975", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1654", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a5c3b58-adb8-444d-a65d-5c43fb578898", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1653, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001653__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1654-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1654, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1655, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1976", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1655", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7e9fa82-1525-41a6-859c-52a5f147dd01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1654, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001654__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1655-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1655, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1656, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1977", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1656", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9413ea85-207e-4342-b499-35ff23f6794a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1655, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001655__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1656-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1656, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1657, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1978", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1657", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67abadbf-eb6d-4b1f-8aaa-1db69e2f7f56", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 143955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1656, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001656__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1657-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1657, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1658, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1979", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1658", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fff9a54-dd5a-45dc-ac03-c22e724712ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1657, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001657__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1658-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1658, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1659, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1980", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1659", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f444c38d-ff9a-43b2-af78-cb7bf18c9c98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1658, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001658__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1659-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1659, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "143984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1660, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1981", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1660", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1d2a08b-89d5-4bd8-9bcd-2fc554c3cce1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1659, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001659__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1660-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1660, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1661, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1982", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1661", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81c74035-e035-4643-8005-18a0de3709b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1660, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001660__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1661-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1661, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1662, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1983", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1662", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cdb4855c-db2a-4abb-ad4c-a889552a0725", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1661, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001661__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1662-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1662, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1663, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1984", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1663", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ca29ab8-a12d-4fc9-a58f-5a733abbc16b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1662, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001662__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1663-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1663, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1664, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1985", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1664", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "436c826c-86be-49da-ab0b-288c0769fbe8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1663, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001663__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1664-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1664, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1665, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1986", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1665", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d1d1029-e7c3-4fae-aee1-ee23997ab688", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1664, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001664__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1665-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1665, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1666, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1987", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1666", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff4d6965-1843-48cc-8ffe-55e63384e3e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1665, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001665__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1666-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1666, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1667, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1988", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1667", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6d9f585-ea29-4d33-9da3-38f1404483f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1666, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001666__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1667-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1667, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1668, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1989", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1668", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f96056fa-ee8a-4326-9dc1-16fe4fe78e90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1667, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001667__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1668-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1668, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1669, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1990", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1669", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a93e051c-67cc-461d-9f7d-71873f138e83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 144986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1668, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001668__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1669-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1669, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1670, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1991", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1670", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f394af4c-229a-4111-a221-a58cf0d2a9e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1669, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001669__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1670-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1670, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "144953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1671, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1992", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1671", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8656cb7a-272d-4760-95e0-4179fba4ff15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1670, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001670__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1671-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1671, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1672, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1993", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1672", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e987d45-bd8d-4a2c-8e2c-f7a04f1bdbfd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1671, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001671__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1672-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1672, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1673, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1994", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1673", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9741342d-89b5-4e95-889b-856a7c764834", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1672, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001672__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1673-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1673, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1674, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1995", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1674", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7bae9ef-18a6-4970-af5d-6806547aeaf8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1673, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001673__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1674-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1674, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1675, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1996", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1675", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2eefbd05-8a16-4294-b41c-d8cb03deeaf2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1674, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001674__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1675-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1675, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1676, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1997", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1676", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1e80105-7943-4aaa-999b-16cc7159e534", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1675, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001675__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1676-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1676, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1677, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1998", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1677", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5d9ab0a-ff4a-49dc-a593-ad440f8b4383", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1676, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001676__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1677-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1677, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1678, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "1999", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1678", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "deacbe8f-b7df-46a5-94bb-1a53700f95e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1677, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001677__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1678-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1678, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1679, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2000", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1679", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f6a2bc1-b22f-4d08-a763-e5b5274c84d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1678, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001678__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1679-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1679, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1680, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2001", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1680", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56fe32e2-3c62-4c08-bff0-a3674eb86542", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 145940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1679, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001679__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1680-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1680, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1681, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2002", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1681", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "097f4c52-af7e-4e49-88c7-743b16f84dd2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1680, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001680__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1681-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1681, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1682, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2003", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1682", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "932691a2-be00-4bbe-816b-7dafbf73f67d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1681, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001681__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1682-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1682, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "145984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1683, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2004", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1683", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7be1fdf4-2e83-480d-bdec-3b2107b9dad0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1682, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001682__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1683-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1683, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1684, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2005", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1684", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86cd2f21-83da-4832-9ccf-4614cddd302f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1683, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001683__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1684-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1684, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1685, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2006", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1685", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a8fb39a0-7614-41b2-a0e4-c722407101e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1684, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001684__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1685-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1685, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1686, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2007", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1686", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d451dabd-29b2-4c6c-8ea8-a9cdf9da2b6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1685, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001685__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1686-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1686, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1687, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2008", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1687", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5dc6bf4f-5b61-495e-9fff-61a13e419b27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1686, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001686__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1687-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1687, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1688, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2009", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1688", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90026d1b-8934-47aa-8f25-6c5a0c150824", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1687, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001687__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1688-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1688, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1689, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2010", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1689", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c0eded0-e079-47bf-a232-d4c7c0e797f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1688, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001688__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1689-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1689, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1690, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2011", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1690", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29ddf52b-7c0b-4298-97e2-ef2dd8c23c80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1689, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001689__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1690-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1690, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1691, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2012", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1691", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5befa66f-715a-48c2-a47f-626e1f545655", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1690, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001690__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1691-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1691, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1692, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2013", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1692", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84cf9702-3d74-4910-8794-866f55d3d501", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 146986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1691, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001691__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1692-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1692, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1693, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2014", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1693", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e2f06b7-d70e-46ab-a435-ce2fb69da2ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1692, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001692__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1693-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1693, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "146937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1694, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2015", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1694", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10de3ac0-6dc6-4b4f-94e7-2fe00d4dac5a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1693, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001693__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1694-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1694, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1695, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2016", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1695", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1535b8f2-3471-4743-884a-5d65eca18597", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1694, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001694__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1695-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1695, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1696, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2017", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1696", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec33594d-f39d-4915-959d-8a8948b493cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1695, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001695__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1696-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1696, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1697, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2018", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1697", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "099248cb-1c7f-48cd-9f6f-482cb0ee41bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1696, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001696__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1697-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1697, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1698, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2019", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1698", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eaefe8fd-6b72-4133-8aa7-2ee3f4ed0ed0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1697, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001697__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1698-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1698, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1699, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2020", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1699", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee9d9f9f-ff8c-41a8-a2aa-b89af904c79a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1698, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001698__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1699-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1699, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1700, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2021", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1700", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a18d84f1-e4c9-42d0-a544-bc08652e908b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1699, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001699__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1700-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1700, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1701, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2022", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1701", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f18154e7-b8b0-488b-a7f5-b9c78499d77b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1700, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001700__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1701-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1701, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1702, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2023", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1702", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72b188c0-60bb-4fba-a968-d82e526bf471", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1701, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001701__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1702-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1702, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1703, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2024", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1703", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2f476b0-322d-4b5a-a3e5-533ffe490b1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 147940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1702, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001702__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1703-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1703, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1704, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2025", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1704", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2d290c3-eb19-4122-bd5e-b30b807f1fdf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1703, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001703__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1704-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1704, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1705, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2026", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1705", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "618f34f3-4fac-456e-a236-8516d1ae3f4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1704, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001704__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1705-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1705, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "147984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1706, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2027", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1706", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b7e6c55-7474-467a-baeb-5af9f4a1f7f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1705, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001705__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1706-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1706, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1707, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2028", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1707", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1c70050-35d4-4bfd-b98c-256cb50096f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1706, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001706__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1707-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1707, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1708, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2029", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1708", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9698cde8-e1be-431f-a480-b55d92c0e54f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1707, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001707__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1708-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1708, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1709, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2030", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1709", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ee15036-49e8-4431-bc67-e3174b0e6ef0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1708, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001708__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1709-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1709, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1710, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2031", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1710", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3232ce3f-0df0-48c1-bf0d-dd468ca52865", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1709, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001709__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1710-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1710, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1711, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2032", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1711", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5eae401a-2b85-47a6-936d-c8c28a05705d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1710, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001710__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1711-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1711, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1712, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2033", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1712", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c82f5e3-0729-4e17-8cf0-cd87af16704c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1711, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001711__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1712-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1712, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1713, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2034", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1713", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baf966a0-c266-445a-95f6-ba7ca9cac81c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1712, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001712__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1713-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1713, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1714, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2035", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1714", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ed2fd21-cf54-4ac4-87b8-f78c424e5a15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1713, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001713__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1714-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1714, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1715, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2036", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1715", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28dfed20-7c43-4205-bafb-fcbe93e59fff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 148986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1714, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001714__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1715-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1715, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1716, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2037", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1716", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea8c601a-4d87-476d-9ce1-795285510a47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1715, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001715__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1716-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1716, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "148937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1717, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2038", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1717", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c610abc4-06c0-4f63-aab4-7ae2a707f5ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1716, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001716__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1717-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1717, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1718, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2039", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1718", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "705c8a3a-964b-4392-97b9-0aff3de20fa5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1717, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001717__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1718-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1718, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1719, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2040", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1719", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a603981b-01f9-4bcb-9597-2145cebd1b0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1718, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001718__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1719-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1719, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1720, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2041", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1720", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cf3b7e8-7dd4-49f2-8872-7d484d7c2cf2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1719, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001719__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1720-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1720, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1721, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2042", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1721", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9048693e-af9b-475f-93dd-55b6e40d8730", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1720, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001720__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1721-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1721, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1722, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2043", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1722", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "998a6586-20eb-4bb3-ab4a-734e3f8ce935", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1721, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001721__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1722-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1722, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1723, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2044", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1723", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6b79013-955b-4b91-be59-610c5d06545a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1722, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001722__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1723-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1723, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1724, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2045", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1724", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92026a83-fae1-430d-ac97-753a6818c61b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1723, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001723__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1724-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1724, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1725, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2046", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1725", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd4d52eb-e18e-4557-8870-57de2e66406c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1724, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001724__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1725-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1725, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1726, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2047", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1726", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7aed7c0-0270-4e7d-b83b-ba3ffbe2762e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 149940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1725, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001725__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1726-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1726, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1727, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2048", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1727", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15c0a2eb-02f0-4b24-9d57-9d40b872131f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1726, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001726__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1727-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1727, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1728, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2049", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1728", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7240aee-c236-44c5-a575-e24b1c1cb64f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1727, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001727__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1728-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1728, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "149984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1729, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2050", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1729", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4be5a170-dbf0-4839-85a7-c50d3c432658", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1728, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001728__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1729-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1729, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1730, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2051", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1730", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ef380a3-691f-421c-8ee6-9dc3460e3533", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1729, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001729__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1730-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1730, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1731, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2052", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1731", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "807de92c-1ff6-4856-9026-8cc1ae50f23f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1730, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001730__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1731-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1731, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1732, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2053", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1732", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16a9f463-1593-4297-b57e-b500d9ff8730", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1731, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001731__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1732-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1732, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1733, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2054", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1733", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fdf34d5-d73b-4a7e-91a5-832006c0a45a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1732, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001732__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1733-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1733, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1734, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2055", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1734", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a6d3f89-fd19-46cc-b810-69eea19423bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1733, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001733__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1734-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1734, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1735, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2056", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1735", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe0a1256-828a-45d0-a830-fc66c8dabda1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1734, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001734__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1735-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1735, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1736, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2057", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1736", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50cb4350-3e82-4df9-8968-ad65abb38f42", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1735, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001735__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1736-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1736, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1737, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2058", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1737", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc14f5af-3a3e-40f3-a67b-ec9635bd0afb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1736, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001736__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1737-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1737, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1738, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2059", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1738", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d12a8f7b-562b-4ec4-8edc-7a5b59d1ef1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 150971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1737, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001737__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1738-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1738, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1739, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2060", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1739", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcdac7b3-3218-49bb-9f74-f690a62bb8fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1738, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001738__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1739-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1739, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "150937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1740, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2061", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1740", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "946147fc-1091-4a00-ba72-d0277ef94b5a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1739, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001739__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1740-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1740, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1741, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2062", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1741", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "103acd9c-5683-40fc-bd8a-f2ba8311c8aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1740, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001740__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1741-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1741, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1742, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2063", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1742", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b678aab-4d0e-46d4-8c2f-215c919a9cf5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1741, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001741__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1742-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1742, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1743, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2064", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1743", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2e67cd4-d1be-40b1-bd5b-e241f9de4f61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1742, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001742__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1743-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1743, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1744, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2065", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1744", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d352e07-216b-4119-938a-bd9b93c48985", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1743, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001743__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1744-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1744, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1745, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2066", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1745", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d51fd0ee-84c3-46d5-8fa4-295edcbf3e4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1744, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001744__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1745-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1745, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1746, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2067", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1746", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09004f3a-4f2a-4692-980b-cfa20359c55d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1745, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001745__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1746-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1746, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1747, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2068", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1747", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97f865f3-6abc-4884-9440-d3239c3b5e22", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1746, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001746__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1747-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1747, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1748, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2069", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1748", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33c5cbc2-6a45-4cc4-a7ac-1315e2452e71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1747, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001747__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1748-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1748, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1749, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2070", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1749", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98b3db14-9043-43b2-9be6-a0d824e6fa95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 151940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1748, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001748__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1749-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1749, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1750, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2071", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1750", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01c659e8-61f2-46a3-aea9-24695aefeaf7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1749, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001749__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1750-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1750, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1751, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2072", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1751", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7f9b628-afeb-4b37-9b02-6f4bd2f0b720", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1750, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001750__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1751-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1751, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "151968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1752, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2073", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1752", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e6e02bb-01aa-437d-938a-7946910af7eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1751, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001751__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1752-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1752, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1753, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2074", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1753", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efd17d6f-02da-4936-bd55-4ad3cd23fdbe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1752, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001752__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1753-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1753, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1754, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2075", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1754", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90f60f99-e8c4-459b-9495-f6ad5bee5269", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1753, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001753__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1754-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1754, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1755, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2076", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1755", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ba6b883-4c76-4111-b18d-59bad01b625b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1754, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001754__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1755-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1755, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1756, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2077", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1756", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04b8b2a9-2898-4883-9334-98421fb9d76e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1755, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001755__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1756-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1756, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1757, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2078", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1757", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efce38a8-8d69-4b29-818b-5ac1d9b09159", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1756, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001756__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1757-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1757, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1758, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2079", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1758", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c68324e3-8841-479c-ae2b-7624b8895c76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1757, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001757__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1758-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1758, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1759, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2080", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1759", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cfb540b-d0b3-4b56-895b-bbe5aa28ed35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1758, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001758__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1759-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1759, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1760, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2081", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1760", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09b3543d-79ce-4d84-b551-573ea1f3709a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1759, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001759__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1760-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1760, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1761, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2082", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1761", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64225e14-5ff1-446a-b285-ce1bc0ba588c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 152971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1760, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001760__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1761-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1761, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1762, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2083", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1762", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7622a80f-cfb6-4e70-8d70-ae283484c717", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1761, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001761__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1762-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1762, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "152921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1763, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2084", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1763", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a6f405a-fce5-4774-a5cc-7e4362f62a76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1762, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001762__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1763-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1763, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1764, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2085", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1764", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f632c644-4ede-4b8c-bb30-2016cd5fb364", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1763, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001763__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1764-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1764, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1765, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2086", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1765", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfd01a8b-eb7e-4375-99fb-6fe6a1be6136", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1764, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001764__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1765-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1765, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1766, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2087", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1766", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe406f3a-b9e7-40dd-b798-4914804b5d10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1765, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001765__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1766-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1766, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1767, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2088", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1767", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed16b29d-2e59-49e1-9992-b5d4e35cf230", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1766, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001766__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1767-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1767, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1768, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2089", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1768", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f8d432a-9e9d-43c0-a0e2-2646f2b27646", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1767, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001767__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1768-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1768, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1769, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2090", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1769", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61982a60-c0af-49ef-af2c-cebd8ad59f2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1768, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001768__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1769-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1769, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1770, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2091", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1770", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a7a7a6f-e5e9-4f9c-8a53-bbfc3f92c66f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1769, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001769__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1770-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1770, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1771, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2092", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1771", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57ceb90c-125b-40eb-a4d1-91ed1bc1c757", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1770, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001770__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1771-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1771, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1772, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2093", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1772", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69d55034-9e19-4053-9226-20b732eb0f94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 153924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1771, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001771__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1772-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1772, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1773, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2094", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1773", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4aa4765a-3f5b-4e11-99a5-8420a74e40f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1772, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001772__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1773-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1773, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1774, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2095", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1774", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71dd3e34-b123-4ff9-b04f-4624371bb8e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1773, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001773__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1774-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1774, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "153968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1775, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2096", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1775", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "108578b3-168e-4658-8b1b-b6127ab170fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1774, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001774__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1775-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1775, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1776, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2097", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1776", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8e09e74-ac3a-45d5-aea1-3482e55ae0a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1775, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001775__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1776-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1776, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1777, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2098", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1777", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90f3a6e7-ccd6-440a-a24a-731735685f03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1776, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001776__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1777-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1777, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1778, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2099", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1778", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ea947cf-2b2a-4f82-a89e-c06d9d52bd4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1777, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001777__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1778-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1778, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1779, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2100", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1779", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "661909ad-aca7-46de-9f12-a77fe388ba2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1778, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001778__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1779-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1779, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1780, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2101", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1780", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec508a65-0b49-4b5e-bdcb-a9b3cad5a316", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1779, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001779__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1780-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1780, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1781, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2102", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1781", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4886ff36-2402-434a-b7a9-08a7369cb863", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1780, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001780__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1781-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1781, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1782, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2103", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1782", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a96919ca-6fa7-4526-bd69-f8b73ad09f35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1781, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001781__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1782-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1782, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1783, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2104", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1783", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fef0b7ac-e1a5-43eb-9ff7-f0112264c5fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1782, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001782__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1783-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1783, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1784, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2105", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1784", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8d1fb00-d47d-427e-bafd-f2985f98b57d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 154971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1783, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001783__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1784-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1784, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1785, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2106", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1785", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f87305c6-96e1-4cc9-8ae8-478911edbdda", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1784, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001784__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1785-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1785, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "154921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1786, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2107", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1786", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe960711-fbf5-42fe-b62d-73703dd648ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1785, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001785__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1786-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1786, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1787, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2108", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1787", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89b1f536-ff8b-45ca-bceb-7e9ad616f911", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1786, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001786__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1787-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1787, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1788, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2109", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1788", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e40cb83-aecf-4631-a027-61d9d0a1e517", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1787, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001787__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1788-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1788, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1789, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2110", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1789", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63fb9053-62dc-4f8c-bd06-0031bd51d1e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1788, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001788__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1789-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1789, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1790, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2111", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1790", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1881d1ac-f83f-44b5-895a-7968c4bde1a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1789, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001789__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1790-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1790, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1791, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2112", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1791", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3665e9d7-331b-4cf7-89ce-775238dc613a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1790, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001790__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1791-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1791, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1792, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2113", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1792", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1b00abe-92a5-4b91-8326-92f7eab94c2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1791, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001791__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1792-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1792, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1793, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2114", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1793", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4566222f-cff8-4668-9707-1d6d9e87f885", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1792, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001792__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1793-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1793, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1794, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2115", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1794", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "468ce254-6e2e-4278-afa6-0d3df50cf879", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1793, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001793__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1794-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1794, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1795, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2116", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1795", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac35a14d-d6d2-4317-bdbb-4a8b9c11b401", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 155924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1794, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001794__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1795-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1795, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1796, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2117", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1796", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2f4f71f-e879-437e-9a81-d22f5812aacc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1795, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001795__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1796-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1796, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1797, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2118", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1797", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3f630c5-9c79-4eed-9ef6-f7d0d59be5bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1796, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001796__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1797-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1797, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "155968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1798, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2119", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1798", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fea26090-fad7-4788-a49f-35a766acde68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1797, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001797__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1798-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1798, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1799, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2120", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1799", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66237c7f-f7f1-4bfb-a0f6-89a98d694c1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1798, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001798__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1799-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1799, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1800, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2121", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1800", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "954f50d9-eb7c-4f77-9887-c343d1bb7843", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1799, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001799__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1800-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1800, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1801, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2122", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1801", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a19e12d8-ed7b-4ecd-8f48-ee34180183de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1800, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001800__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1801-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1801, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1802, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2123", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1802", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8057e801-2387-4f48-aa2b-712e20986b4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1801, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001801__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1802-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1802, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1803, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2124", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1803", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bc324c1-5d9b-4f63-877d-d19f0e684959", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1802, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001802__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1803-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1803, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1804, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2125", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1804", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "185d42c9-f869-4496-b041-accbfb09bb53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1803, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001803__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1804-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1804, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1805, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2126", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1805", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0972e93a-aeef-4796-a07d-d5992c9e405f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1804, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001804__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1805-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1805, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1806, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2127", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1806", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ff2b1fe-5b54-43fc-875e-512329d4dfa8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1805, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001805__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1806-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1806, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1807, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2128", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1807", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b55dd2b-d9b9-4db5-b744-9d6bf1184464", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 156955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1806, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001806__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1807-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1807, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1808, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2129", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1808", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2de69b4-796f-42bb-8c2f-ec4f7b944a5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1807, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001807__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1808-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1808, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "156921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1809, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2130", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1809", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1ddb8c9-cbd7-4ed1-92b1-452832d78ae7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1808, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001808__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1809-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1809, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1810, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2131", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1810", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a131af14-1aa1-409a-9dac-a7eb4ae5284c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1809, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001809__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1810-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1810, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1811, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2132", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1811", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91c2d0a9-70f1-4621-9ed9-d6fb5d7376a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1810, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001810__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1811-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1811, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1812, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2133", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1812", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c37c4ae-7134-443d-a31d-e1db0711f8f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1811, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001811__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1812-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1812, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1813, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2134", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1813", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccbb6aba-7470-41c2-92e9-c01fcdf4bea6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1812, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001812__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1813-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1813, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1814, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2135", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1814", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89fba4aa-7532-470b-899d-b2eefc030971", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1813, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001813__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1814-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1814, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1815, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2136", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1815", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03a21a0a-aff4-4aaa-a473-ca6c3088ade5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1814, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001814__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1815-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1815, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1816, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2137", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1816", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f722b952-a0e1-4c2e-86da-a34238df1575", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1815, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001815__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1816-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1816, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1817, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2138", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1817", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "063e6bed-f22d-4d70-a26e-7300f81a7491", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1816, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001816__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1817-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1817, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1818, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2139", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1818", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85be0991-6d75-452c-8ba9-7838b6ee7890", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 157924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1817, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001817__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1818-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1818, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1819, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2140", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1819", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfc72418-7e08-4c8c-bb50-7c26cc90eb2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1818, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001818__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1819-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1819, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1820, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2141", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1820", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d128fd1-6ab0-43f7-b9c1-f31e880fca32", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1819, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001819__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1820-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1820, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "157953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1821, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2142", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1821", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fc028db-2286-4272-9e06-b137177aaad5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1820, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001820__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1821-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1821, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1822, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2143", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1822", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34dd1cf9-a749-4587-bfe2-569de800bc5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1821, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001821__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1822-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1822, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1823, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2144", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1823", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "954f11ac-442e-4c61-9d14-ea52365e7322", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1822, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001822__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1823-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1823, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1824, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2145", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1824", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "804fbba6-b378-4192-bed2-9985a333dbe6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1823, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001823__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1824-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1824, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1825, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2146", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1825", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f942425-eecf-4765-894f-707276729ed0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1824, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001824__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1825-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1825, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1826, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2147", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1826", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10d6c901-42fa-4309-8760-b551a9787e97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1825, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001825__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1826-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1826, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1827, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2148", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1827", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d78b0320-596d-44a8-8b5f-c9420bf0524a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1826, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001826__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1827-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1827, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1828, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2149", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1828", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0bf5843b-8e90-4b8a-af1b-6ec5845829fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1827, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001827__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1828-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1828, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1829, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2150", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1829", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2a0a105-2831-4499-8f76-da4b640ec15a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1828, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001828__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1829-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1829, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1830, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2151", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1830", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecb46131-0989-4b0b-af08-0e3db6539d24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 158955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1829, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001829__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1830-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1830, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1831, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2152", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1831", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fcbd5ee-e35a-4c49-a577-abc3e828219e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1830, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001830__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1831-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1831, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "158906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1832, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2153", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1832", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c26036dc-166c-4f6c-9120-bb95a077d442", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1831, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001831__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1832-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1832, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1833, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2154", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1833", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e16a4617-871a-4453-973e-c01ed496c39e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1832, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001832__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1833-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1833, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1834, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2155", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1834", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd319f26-5855-4593-bec0-3da40e09fcd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1833, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001833__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1834-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1834, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1835, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2156", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1835", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32c3a8e2-3b81-4150-b9c7-6e6e1f31311e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1834, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001834__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1835-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1835, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1836, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2157", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1836", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a69cb3fa-67c2-411b-bd3f-ac52819b98c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1835, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001835__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1836-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1836, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1837, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2158", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1837", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8342c073-674f-44ca-b0af-1141f7b116c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1836, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001836__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1837-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1837, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1838, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2159", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1838", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53aab46d-a3b2-4ad0-9817-e803b8415d0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1837, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001837__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1838-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1838, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1839, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2160", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1839", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fffc33d-ac10-44a5-aef2-ebfcfd3674ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1838, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001838__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1839-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1839, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1840, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2161", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1840", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8af5a6a7-1a61-4158-90e3-25ca422caf41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1839, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001839__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1840-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1840, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1841, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2162", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1841", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb58beb4-736e-43cd-a137-ad81c0e3229d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 159908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1840, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001840__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1841-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1841, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1842, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2163", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1842", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81848d2a-8e6e-4bf5-91a6-96b53fd48b89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1841, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001841__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1842-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1842, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1843, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2164", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1843", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc64ffa3-2e01-4a0c-bdb8-47c027154590", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1842, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001842__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1843-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1843, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "159953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1844, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2165", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1844", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18e52608-4dd0-45ad-8744-6da4d5787b73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1843, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001843__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1844-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1844, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1845, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2166", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1845", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5613bbbb-b8fe-4768-9aa5-0b16029692b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1844, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001844__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1845-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1845, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1846, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2167", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1846", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78dc1bea-f51d-46df-94cf-f95566f58583", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1845, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001845__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1846-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1846, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1847, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2168", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1847", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f866fbe-dbc6-4965-b064-391cb671aadf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1846, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001846__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1847-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1847, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1848, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2169", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1848", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "875d36fa-7b32-4c0e-8f11-50c2332dee04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1847, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001847__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1848-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1848, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1849, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2170", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1849", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c667c7a-c7e5-4d21-9666-f3e61bc3ab51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1848, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001848__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1849-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1849, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1850, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2171", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1850", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da1f79cd-a2a4-48d3-a182-36280f362479", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1849, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001849__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1850-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1850, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1851, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2172", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1851", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22f51caa-5432-4031-b376-540c87e3f7e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1850, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001850__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1851-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1851, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1852, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2173", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1852", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7bd5696-fe32-4c0f-860f-9641f679d5ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1851, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001851__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1852-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1852, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1853, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2174", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1853", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8992c5dd-1eaa-41ae-9a18-630010e6e1ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 160955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1852, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001852__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1853-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1853, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1854, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2175", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1854", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f2a6e47-c0f6-43aa-add3-1c94ca36176e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1853, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001853__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1854-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1854, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "160906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1855, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2176", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1855", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f721090-0d1b-46c5-a85f-043aab2685d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1854, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001854__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1855-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1855, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1856, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2177", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1856", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ca43ebb-b945-40f4-b3a2-6c0b63d61d98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1855, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001855__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1856-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1856, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1857, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2178", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1857", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56b4ac88-5678-44e4-97e9-6f81c232aa9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1856, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001856__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1857-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1857, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1858, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2179", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1858", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cd5b594-6483-4968-88af-197948080549", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1857, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001857__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1858-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1858, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1859, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2180", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1859", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c7813e9-c68b-4479-8714-0f30a0066a2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1858, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001858__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1859-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1859, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1860, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2181", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1860", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b288b2b-70f8-4874-b1ba-3f0a4de11435", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1859, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001859__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1860-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1860, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1861, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2182", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1861", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2eb4cabf-4be0-4b07-bbb4-8ec658fce95b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1860, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001860__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1861-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1861, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1862, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2183", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1862", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01cbffb0-8de1-4da3-8916-a001a809460a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1861, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001861__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1862-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1862, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1863, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2184", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1863", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7796f587-4abf-4f9c-9914-067e35b8064e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1862, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001862__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1863-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1863, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1864, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2185", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1864", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5be2d7be-79ec-4d4e-8b0c-cbd8757bff2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1863, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001863__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1864-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1864, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1865, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2186", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1865", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31b34a9f-29bb-42e9-bad8-53a8e8969e7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 161986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1864, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001864__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1865-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1865, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1866, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2187", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1866", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcac3bc9-dc84-49ce-8456-505e9b078562", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1865, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001865__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1866-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1866, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "161953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1867, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2188", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1867", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b1f8ec2-3367-4175-96e8-73e4600c9fb8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1866, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001866__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1867-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1867, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1868, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2189", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1868", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8add75bc-5bd9-40ab-85c7-f17003fc1a0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1867, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001867__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1868-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1868, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1869, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2190", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1869", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8189053c-31ae-45de-9b0c-48fcae410055", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1868, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001868__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1869-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1869, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1870, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2191", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1870", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9eeb559-e034-42b3-ac5d-6a54be292af2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1869, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001869__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1870-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1870, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1871, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2192", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1871", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6008e24a-3a80-4bd1-98c1-c2f10df359d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1870, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001870__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1871-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1871, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1872, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2193", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1872", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7953212d-5d8a-42bc-bdd8-4a3af23c3624", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1871, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001871__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1872-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1872, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1873, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2194", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1873", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3765f35e-68eb-4e22-a04c-8b6c58e71478", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1872, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001872__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1873-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1873, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1874, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2195", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1874", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e39ef990-dee9-470b-863a-780a1a202874", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1873, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001873__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1874-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1874, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1875, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2196", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1875", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72aac0ff-5c1e-4c36-a3de-8660837cca5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1874, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001874__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1875-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1875, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1876, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2197", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1876", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67160fb9-4a33-4ddd-a069-703d59ca9340", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 162955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1875, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001875__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1876-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1876, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1877, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2198", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1877", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69d89764-97e3-4cbf-84c5-604d15777731", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1876, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001876__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1877-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1877, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1878, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2199", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1878", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26ccbf80-e415-49f3-b897-abf882e1eec9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1877, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001877__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1878-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1878, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "162984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1879, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2200", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1879", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1167ef1-0853-407a-953a-f61f5d1c9619", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1878, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001878__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1879-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1879, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1880, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2201", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1880", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e50a9ef-a2b8-40de-898a-c0bfd4a938ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1879, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001879__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1880-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1880, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1881, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2202", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1881", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "499e1150-c91d-4dde-86f3-5760aa59c941", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1880, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001880__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1881-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1881, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1882, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2203", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1882", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "383f914e-e63a-4993-b37a-891d4d740108", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1881, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001881__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1882-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1882, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1883, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2204", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1883", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "951e94c5-10d8-4c70-9410-586aead521f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1882, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001882__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1883-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1883, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1884, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2205", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1884", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7960b1a-6d50-4e91-adce-40374af37cfa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1883, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001883__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1884-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1884, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1885, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2206", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1885", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4336356c-4ab5-4f68-abc9-d03aad7dfd60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1884, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001884__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1885-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1885, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1886, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2207", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1886", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a66c39de-95a9-4f9d-b682-c3090ff2c189", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1885, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001885__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1886-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1886, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1887, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2208", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1887", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ceeab172-1e35-4a56-994c-e456092fb48d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1886, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001886__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1887-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1887, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1888, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2209", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1888", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94df7174-c10d-4c57-8e98-2250c4794a4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 163986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1887, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001887__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1888-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1888, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1889, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2210", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1889", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d101ae4-29c0-4e9f-9bd3-001c11bc9cea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1888, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001888__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1889-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1889, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "163937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1890, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2211", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1890", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5ee18fd-3452-4580-885f-794b0a46c8c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1889, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001889__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1890-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1890, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1891, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2212", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1891", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "edf50af0-19c4-476a-aec7-822984d0d499", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1890, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001890__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1891-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1891, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1892, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2213", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1892", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c0b539e-5f23-4ada-aaad-83ced23b4576", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1891, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001891__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1892-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1892, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1893, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2214", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1893", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f370b5e2-72cd-4730-8bf8-f404ecd9c5b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1892, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001892__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1893-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1893, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1894, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2215", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1894", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc35c3ec-0685-4f54-8d9a-ec21419982da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1893, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001893__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1894-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1894, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1895, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2216", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1895", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb8f70d7-de8b-44ec-b3cf-bf1cbd115376", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1894, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001894__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1895-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1895, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1896, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2217", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1896", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12485cf2-988a-42fe-9dff-89381aae099d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1895, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001895__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1896-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1896, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1897, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2218", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1897", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e7978344-b418-45cc-b6f2-25a4f0c18f4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1896, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001896__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1897-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1897, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1898, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2219", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1898", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e19ecf62-e85f-4237-a255-c6f1f212d397", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1897, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001897__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1898-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1898, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1899, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2220", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1899", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b304323c-b38f-4884-8428-6a68f03ff70e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 164940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1898, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001898__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1899-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1899, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1900, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2221", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1900", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "251b126c-11b6-4fd7-889d-a9953e678afb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1899, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001899__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1900-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1900, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1901, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2222", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1901", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f76d62b-8e4b-48ff-8ea1-a3c82d5e7419", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1900, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001900__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1901-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1901, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "164984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1902, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2223", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1902", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44816c71-6c3c-4d19-9c96-1223edad9050", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1901, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001901__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1902-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1902, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1903, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2224", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1903", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60034570-5db8-463a-a3a7-4fc71506d1e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1902, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001902__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1903-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1903, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1904, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2225", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1904", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e822e46-53a2-44fe-9804-0afa706973cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1903, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001903__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1904-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1904, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1905, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2226", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1905", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0990005c-2196-4a5f-a988-c38d83750bc4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1904, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001904__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1905-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1905, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1906, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2227", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1906", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1eb8659e-8ef3-49c4-8383-b83b78666906", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1905, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001905__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1906-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1906, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1907, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2228", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1907", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7840ffbf-902b-4dd7-928b-1391afa45f0e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1906, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001906__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1907-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1907, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1908, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2229", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1908", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12321191-7c43-4c3b-a753-1b04301ca1ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1907, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001907__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1908-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1908, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1909, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2230", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1909", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61b3db82-1fc5-4e9a-86e9-03feb05f44b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1908, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001908__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1909-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1909, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1910, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2231", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1910", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5335ed3-e61b-44fe-95fe-2da2a5e17b1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1909, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001909__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1910-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1910, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1911, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2232", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1911", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "005e659c-9a85-4131-b408-426443f4eef7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 165986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1910, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001910__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1911-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1911, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1912, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2233", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1912", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c522fe31-e458-4e72-99a5-0d777c044119", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1911, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001911__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1912-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1912, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "165937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1913, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2234", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1913", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de257756-34ef-4d36-99b0-ad67467733e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1912, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001912__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1913-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1913, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1914, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2235", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1914", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "edef8775-d827-4d27-a0f2-b4828fe45c8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1913, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001913__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1914-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1914, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1915, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2236", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1915", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a387871-f684-46cb-8988-e5239c2cc6d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1914, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001914__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1915-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1915, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1916, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2237", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1916", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d340d236-5fcc-4df0-852f-9ce8a8e25228", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1915, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001915__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1916-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1916, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1917, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2238", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1917", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1dff9d0-6467-4629-8e65-bc5385c7a561", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1916, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001916__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1917-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1917, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1918, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2239", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1918", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58ce9798-5ca1-437d-b106-8dffa6e0e97a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1917, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001917__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1918-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1918, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1919, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2240", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1919", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92783c21-c2f9-40e7-9a82-ad3ed6338819", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1918, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001918__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1919-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1919, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1920, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2241", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1920", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17e0c7f9-f2b0-46bd-8731-f2886913a381", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1919, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001919__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1920-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1920, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1921, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2242", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1921", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98a6c2b0-2272-460c-a5a8-851d26519ee0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1920, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001920__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1921-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1921, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1922, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2243", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1922", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "263ed7c5-efc5-4203-aa38-f4dae6fb24f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 166940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1921, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001921__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1922-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1922, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1923, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2244", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1923", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "291fa5ff-ab40-4924-ab58-a8897be39113", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1922, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001922__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1923-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1923, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1924, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2245", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1924", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d86c6302-ac79-4d99-aafc-2b525fd1c409", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1923, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001923__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1924-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1924, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "166984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1925, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2246", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1925", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "404b96f6-68d9-4d25-b364-c75ce661cc68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1924, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001924__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1925-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1925, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1926, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2247", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1926", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc3f38f1-6c2b-4d34-93a1-db305eb0b463", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1925, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001925__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1926-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1926, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1927, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2248", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1927", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19e9eff7-5ff8-4841-b7f7-d32975406c07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1926, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001926__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1927-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1927, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1928, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2249", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1928", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7dbbfe09-451d-4664-8dac-3f8d14c67388", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1927, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001927__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1928-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1928, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1929, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2250", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1929", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88df6c29-4f04-4ccb-9fa6-4236ef65393e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1928, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001928__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1929-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1929, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1930, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2251", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1930", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5aedd5a0-5cc1-43b1-8597-8d5ead3bb5a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1929, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001929__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1930-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1930, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1931, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2252", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1931", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01a77bbf-5199-429e-9bef-62e3aadbde46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1930, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001930__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1931-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1931, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1932, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2253", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1932", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc8c1cea-920c-4abb-900d-7e9e82bb81d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1931, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001931__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1932-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1932, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1933, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2254", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1933", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9dd2a00-0eda-4106-a888-9d2959c7a75b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1932, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001932__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1933-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1933, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1934, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2255", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1934", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95b07ee4-3cb9-4407-8455-0c7b2bf0a760", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 167971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1933, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001933__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1934-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1934, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1935, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2256", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1935", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d2f5531-468d-4284-a7c3-9f4e86b14bdf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1934, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001934__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1935-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1935, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "167937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1936, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2257", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1936", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1cc1c7e1-127a-4372-92bb-440c48f5fec7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1935, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001935__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1936-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1936, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1937, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2258", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1937", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36697c25-dd46-4e60-a2e8-5fdc232edf5f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1936, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001936__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1937-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1937, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1938, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2259", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1938", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0bbff492-63b4-48b6-8359-6c1ce59554e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1937, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001937__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1938-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1938, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1939, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2260", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1939", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62d128c6-1437-4b33-a1e4-ed0175e3caba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1938, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001938__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1939-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1939, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1940, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2261", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1940", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6289ad45-6bba-4e78-80db-a80785c94616", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1939, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001939__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1940-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1940, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1941, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2262", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1941", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0b054e3-5365-4b68-bfcf-76ccd6f69919", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1940, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001940__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1941-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1941, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1942, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2263", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1942", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbd29c8f-3720-477f-8259-a15acc057778", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1941, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001941__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1942-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1942, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1943, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2264", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1943", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4dccecf-6c6f-4555-a358-cf98762267e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1942, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001942__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1943-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1943, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1944, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2265", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1944", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ea5f211-4971-4885-b0b4-eb809616746f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1943, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001943__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1944-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1944, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1945, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2266", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1945", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d44b4da6-af04-46b0-af0b-d0ff56969125", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 168940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1944, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001944__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1945-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1945, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1946, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2267", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1946", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4886d6d0-4c2b-41bd-bb15-065509d68a19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1945, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001945__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1946-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1946, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1947, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2268", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1947", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c7b650a-2f2d-4ff7-a6dc-cafa924812b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1946, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001946__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1947-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1947, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "168968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1948, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2269", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1948", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1be14d30-9407-4d1d-bc32-97e8d9411aba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1947, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001947__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1948-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1948, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1949, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2270", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1949", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2f08e48-7d1a-4876-bbe9-c83f8398fb3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1948, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001948__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1949-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1949, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1950, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2271", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1950", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eccb8d9f-af1f-4ad4-9203-21795d9f090c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1949, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001949__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1950-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1950, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1951, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2272", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1951", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb31250a-57d8-4917-8331-fec54bf45e59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1950, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001950__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1951-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1951, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1952, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2273", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1952", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5ac2114-aeaf-4305-a457-fb48ba295662", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1951, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001951__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1952-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1952, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1953, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2274", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1953", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ee7bd08-f859-4eef-9fac-8b07405a700e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1952, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001952__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1953-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1953, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1954, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2275", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1954", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92920570-474b-4aa6-98a0-157aa5c7af99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1953, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001953__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1954-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1954, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1955, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2276", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1955", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "235a8d24-f3ed-4aee-9c15-a1e998e62c12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1954, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001954__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1955-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1955, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1956, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2277", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1956", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84e2cea4-3ea3-48fe-8470-266ebe92bf4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1955, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001955__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1956-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1956, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1957, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2278", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1957", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89e505c2-1a0c-46be-be58-aa2e26940bc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 169971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1956, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001956__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1957-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1957, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1958, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2279", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1958", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc6520e5-285b-41b3-bf51-fcb06325544a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1957, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001957__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1958-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1958, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "169921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1959, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2280", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1959", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a6dc6de-a029-4d76-96e0-60af42a9b04c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1958, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001958__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1959-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1959, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1960, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2281", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1960", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c2d1286-873c-4267-a693-22269dfa3c18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1959, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001959__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1960-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1960, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1961, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2282", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1961", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "497757a4-729e-400c-a2ac-90397a017ff2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1960, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001960__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1961-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1961, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1962, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2283", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1962", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e6a04fe-1dad-4e82-ac9f-9107ef817364", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1961, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001961__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1962-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1962, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1963, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2284", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1963", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43b0d337-90c9-40e3-a6a1-2274a8b1a55e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1962, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001962__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1963-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1963, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1964, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2285", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1964", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d5c56f3-0ae6-43c6-ba38-8c0f7e7b9436", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1963, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001963__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1964-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1964, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1965, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2286", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1965", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be46319e-9acd-41f1-8f60-de3b656825f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1964, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001964__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1965-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1965, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1966, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2287", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1966", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9dff2c8-abc9-4e93-a0da-f8f93bffe736", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1965, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001965__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1966-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1966, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1967, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2288", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1967", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec65340a-ee08-4c62-9908-2f23a28b2f33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1966, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001966__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1967-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1967, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1968, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2289", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1968", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84b9ce02-2034-47cc-a4de-7f57a9e781a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 170924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1967, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001967__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1968-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1968, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1969, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2290", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1969", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "616fd006-10b8-4ec7-9ac6-b1a9008bfb3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1968, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001968__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1969-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1969, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1970, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2291", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1970", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a7413d5-4a8a-4d4e-a48c-a98672052639", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1969, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001969__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1970-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1970, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "170968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1971, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2292", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1971", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27247379-3913-4b22-88da-7ffbd230ff5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1970, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001970__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1971-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1971, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1972, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2293", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1972", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc770291-e9af-4b9f-87e5-66825706db6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1971, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001971__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1972-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1972, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1973, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2294", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1973", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28bb3e35-3da3-4470-9106-f322cf8cf6f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1972, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001972__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1973-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1973, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1974, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2295", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1974", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c0c3346-9d90-4c7d-9da0-50789a7976b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1973, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001973__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1974-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1974, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1975, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2296", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1975", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb214004-c3b0-4705-b6c7-f717b5d4377a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1974, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001974__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1975-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1975, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1976, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2297", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1976", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "acb98ff1-2020-481d-884c-20bd25b7b407", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1975, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001975__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1976-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1976, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1977, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2298", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1977", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3152780-bb84-4280-8863-43ea2e4e59ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1976, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001976__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1977-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1977, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1978, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2299", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1978", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bede962a-56a4-4c4c-9cce-b6aade01ee17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1977, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001977__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1978-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1978, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1979, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2300", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1979", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b720bdd2-979d-4044-94a6-caf1137105da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1978, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001978__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1979-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1979, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1980, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2301", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1980", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4621b18-143b-492c-a6f0-ae4005f34fe7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 171971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1979, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001979__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1980-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1980, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1981, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2302", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1981", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d311458-bcc1-49ad-bc6e-e6725bacdfbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1980, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001980__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1981-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1981, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "171921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1982, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2303", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1982", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccbc4b67-b22c-424f-b8ca-360dc490edd0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1981, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001981__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1982-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1982, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1983, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2304", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1983", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce4b7b4d-3982-4786-8d7f-abc240313055", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1982, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001982__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1983-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1983, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1984, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2305", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1984", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7657a211-720e-49c8-bb00-cb3bc5b1948c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1983, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001983__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1984-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1984, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1985, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2306", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1985", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b910678c-196a-4f69-801f-4d4965210cd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1984, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001984__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1985-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1985, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1986, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2307", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1986", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a749d27-cb61-420e-a42e-2dd3ffc61231", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1985, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001985__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1986-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1986, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1987, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2308", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1987", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f153d48-99ef-4f72-9d98-39b503043713", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1986, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001986__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1987-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1987, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1988, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2309", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1988", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6dd998a-7509-4ee3-a625-6b9dc7fb5828", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1987, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001987__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1988-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1988, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1989, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2310", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1989", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8ce6ba8-d7b7-42a5-b07b-40af180dbb57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1988, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001988__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1989-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1989, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1990, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2311", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1990", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e87f2ab-2f4b-4376-a796-52f56f4a7202", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1989, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001989__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1990-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1990, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1991, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2312", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1991", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "557b4606-8064-4bb9-94b0-d5e8e725a451", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 172924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1990, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001990__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1991-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1991, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1992, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2313", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1992", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f766951-9f3f-4767-8d9b-182444e8f5a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1991, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001991__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1992-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1992, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1993, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2314", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1993", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95a63d80-6b3a-417b-b60d-7abc8980ed1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1992, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001992__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1993-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1993, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "172968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1994, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2315", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1994", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "215bed81-af56-4d56-a806-d3179f3f6cdf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1993, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001993__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1994-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1994, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1995, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2316", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1995", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b4fc682-2e1b-41e5-bc39-ccd546198495", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1994, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001994__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1995-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1995, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1996, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2317", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1996", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b7bdedf-407b-44da-993d-883b86d30af2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1995, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001995__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1996-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1996, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1997, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2318", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1997", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "269abc69-5da1-4807-8810-fe9f3bca5608", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1996, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001996__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1997-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1997, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1998, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2319", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1998", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca9ed6ec-9cd0-4ef9-9ca2-70e64bcb2c75", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1997, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001997__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1998-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1998, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 1999, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2320", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "1999", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f307446-0c93-4eb8-bca4-4c98a143100d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1998, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001998__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-1999-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 1999, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2000, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2321", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2000", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a99e345-a676-49d0-985c-c59b012c0af8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 1999, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000001999__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2000-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2000, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2001, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2322", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2001", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7ca3329-f1bd-433d-821c-61b391cb3daa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2000, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2001-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2001, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2002, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2323", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2002", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf66a3ee-9e2a-437e-8dc0-b214900f369a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2001, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002001__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2002-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2002, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2003, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2324", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2003", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76d56ca5-283d-4f63-9181-4abec11deed0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 173971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2002, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002002__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2003-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2003, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2004, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2325", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2004", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee695a72-7c3d-422d-9017-c5eb0ac94da9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2003, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002003__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2004-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2004, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "173921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2005, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2326", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2005", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "badf2ba5-5341-4479-a388-c5249070b417", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2004, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002004__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2005-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2005, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2006, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2327", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2006", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8277f893-7462-423e-8085-98bd7d3172c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2005, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002005__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2006-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2006, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2007, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2328", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2007", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5da77520-4c49-498e-9a79-423b74464f02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2006, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002006__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2007-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2007, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2008, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2329", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2008", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "560d3968-96ad-489d-8b36-469619abbb29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2007, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002007__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2008-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2008, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2009, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2330", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2009", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d048b53-68a7-4338-ada7-f8e7c0d54026", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2008, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002008__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2009-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2009, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2010, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2331", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2010", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03133f37-e020-4636-9571-b5e605a8fa61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2009, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002009__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2010-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2010, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2011, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2332", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2011", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03dcffbb-f365-43ff-91d8-0a041066cc73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2010, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002010__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2011-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2011, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2012, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2333", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2012", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33fb5ccc-587e-439f-b865-8a61bdab7630", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2011, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002011__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2012-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2012, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2013, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2334", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2013", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "afcbbf41-453d-48ef-820f-95e61e12f67e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2012, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002012__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2013-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2013, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2014, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2335", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2014", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14a26401-44cc-4a97-aeae-2a8e8f7ed6c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 174924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2013, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002013__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2014-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2014, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2015, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2336", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2015", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "238afbf1-87aa-422f-97c3-eec454edd215", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2014, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002014__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2015-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2015, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2016, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2337", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2016", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05b36116-9a37-4b5e-91af-173966207eee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2015, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002015__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2016-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2016, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "174953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2017, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2338", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2017", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56fd7ecf-f6df-44dd-bcbb-b35b2226da10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2016, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002016__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2017-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2017, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2018, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2339", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2018", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20e60cce-5432-4268-8378-4f22b63eb40c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2017, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002017__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2018-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2018, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2019, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2340", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2019", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54603cd7-26a8-4bbb-8219-cefc3759b899", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2018, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002018__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2019-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2019, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2020, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2341", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2020", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0dd0a262-bc9b-4d07-859a-5bdb7afbb83b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2019, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002019__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2020-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2020, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2021, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2342", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2021", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f004edc-4c1c-4243-a13e-ea8813d50671", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2020, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002020__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2021-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2021, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2022, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2343", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2022", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a33dce3-fad8-4986-83aa-890db935c136", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2021, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002021__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2022-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2022, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2023, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2344", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2023", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "634b5051-52d1-4cfd-842f-1c04c68cc9aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2022, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002022__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2023-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2023, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2024, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2345", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2024", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a335a59d-61a8-4e10-8e9a-a4d8509ce885", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2023, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002023__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2024-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2024, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2025, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2346", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2025", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe061312-22ca-499b-a706-43404a85304d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2024, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002024__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2025-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2025, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2026, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2347", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2026", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f6acdea-b86a-4d76-83f7-478138c7cecb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 175955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2025, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002025__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2026-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2026, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2027, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2348", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2027", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f86e076b-6fee-4946-88a6-8de119ae35a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2026, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002026__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2027-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2027, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "175906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2028, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2349", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2028", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f86c84d1-9d70-4605-ad38-5a637cbee9fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2027, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002027__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2028-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2028, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2029, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2350", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2029", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c9c3895b-755f-40f8-957c-c3ab31265a56", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2028, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002028__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2029-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2029, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2030, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2351", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2030", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "608d6d62-1786-4535-b9b6-53c64ad3e3dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2029, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002029__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2030-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2030, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2031, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2352", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2031", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "93e11888-3416-4754-bc7f-6991041a757b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2030, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002030__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2031-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2031, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2032, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2353", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2032", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "581f6298-b56e-438a-a086-48d388a5495f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2031, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002031__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2032-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2032, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2033, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2354", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2033", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16749853-7222-4418-a7ee-64f67582cf3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2032, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002032__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2033-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2033, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2034, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2355", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2034", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "855d19fd-0f58-45a2-a8ae-8ce5ab195ced", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2033, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002033__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2034-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2034, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2035, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2356", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2035", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7883858-a7f3-47fd-baa0-baa85f1334b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2034, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002034__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2035-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2035, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2036, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2357", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2036", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ab78376-9e5d-4ff7-a132-537f3fd0904d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2035, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002035__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2036-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2036, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2037, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2358", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2037", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e12a77e1-95b2-4c8c-bf4f-446340ff6e02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 176908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2036, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002036__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2037-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2037, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2038, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2359", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2038", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9efdb967-6517-4470-a20c-47339a3b3b45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2037, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002037__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2038-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2038, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2039, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2360", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2039", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d4cf64b-148d-4ea2-9a72-a52fb89a85dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2038, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002038__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2039-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2039, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "176953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2040, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2361", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2040", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0dc6c311-259c-4b26-aad2-2235a1abc7e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2039, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002039__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2040-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2040, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2041, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2362", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2041", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "952a4bee-b64b-41e4-839a-0695c5a02ae8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2040, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002040__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2041-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2041, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2042, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2363", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2042", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c89bc93-08a4-4979-9c35-0b9a5cd52b5c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2041, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002041__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2042-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2042, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2043, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2364", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2043", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c016e950-c927-44bb-a3ba-a679aba17abb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2042, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002042__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2043-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2043, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2044, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2365", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2044", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58e5c6bc-6cea-4ced-acf1-8ceaf971a79e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2043, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002043__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2044-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2044, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2045, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2366", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2045", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e0ccaa9-7460-4ccb-90b7-2d15337912c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2044, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002044__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2045-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2045, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2046, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2367", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2046", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29721c25-6b7f-410e-adb4-c0cfb43ad2e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2045, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002045__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2046-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2046, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2047, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2368", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2047", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e6d9918-1ada-431b-ac65-3599f7705584", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2046, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002046__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2047-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2047, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2048, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2369", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2048", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "899525f8-cc60-48c6-a416-cede812eb62b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2047, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002047__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2048-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2048, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2049, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2370", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2049", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b479f230-5d27-4cec-a8bc-c3e14f76bbd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 177955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2048, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002048__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2049-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2049, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2050, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2371", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2050", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dfc575d-0a6a-433d-94ce-27fa37ff3599", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2049, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002049__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2050-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2050, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "177906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2051, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2372", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2051", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89fa00b3-335a-48c8-afd2-9c3a74f5c616", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2050, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002050__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2051-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2051, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2052, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2373", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2052", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7fbb9034-343d-4491-acb7-1befe82c22e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2051, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002051__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2052-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2052, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2053, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2374", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2053", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b30829b-a424-4b56-b4e4-c767af56c454", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2052, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002052__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2053-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2053, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2054, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2375", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2054", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1905d1e-4bb7-43b2-878f-a9fc9cd79a6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2053, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002053__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2054-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2054, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2055, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2376", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2055", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e028886-9d10-4f73-a666-407ef2fae5f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2054, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002054__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2055-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2055, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2056, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2377", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2056", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d82df490-0ab1-4864-8718-808487ed792a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2055, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002055__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2056-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2056, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2057, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2378", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2057", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b83a50f2-f813-4a13-afb3-2ac6e74543ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2056, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002056__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2057-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2057, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2058, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2379", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2058", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "35bc302a-ce89-4a27-95f9-26d548420874", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2057, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002057__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2058-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2058, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2059, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2380", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2059", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9acaef03-4449-4375-ba73-d9bdc1ad0770", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2058, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002058__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2059-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2059, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2060, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2381", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2060", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d036d870-39ba-4632-8077-77a2363f1c7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2059, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002059__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2060-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2060, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2061, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2382", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2061", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5e73399-2247-41cc-9836-033df5829db3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 178986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2060, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002060__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2061-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2061, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2062, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2383", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2062", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b40b3df-083f-4568-b59b-75e24b6c3c89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2061, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002061__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2062-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2062, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "178953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2063, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2384", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2063", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0764eac8-6c3c-4586-8afb-b4e429f208d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2062, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002062__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2063-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2063, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2064, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2385", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2064", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c4cab00-c351-4abf-b266-0269408e924b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2063, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002063__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2064-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2064, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2065, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2386", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2065", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f09b5c5c-1465-48f7-a9ae-01277373a441", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2064, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002064__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2065-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2065, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2066, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2387", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2066", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac93ee85-26a2-4893-8f85-f00d071420b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2065, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002065__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2066-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2066, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2067, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2388", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2067", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2771a6c-8d2a-4505-9e60-3e3edb7f6d59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2066, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002066__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2067-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2067, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2068, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2389", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2068", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76d35156-9663-4062-9cd2-2faee9b52f2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2067, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002067__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2068-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2068, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2069, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2390", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2069", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "445ad2b9-6637-47de-ba01-a04bd45863a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2068, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002068__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2069-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2069, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2070, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2391", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2070", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90adf414-9415-4e1b-9428-2a7560e80075", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2069, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002069__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2070-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2070, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2071, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2392", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2071", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "304309eb-ee9b-469a-983a-28e140f53272", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2070, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002070__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2071-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2071, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2072, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2393", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2072", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "506dc7c3-93cd-46f6-9c60-ed530b550c11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 179955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2071, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002071__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2072-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2072, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2073, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2394", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2073", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2337ccc-3bff-40b0-b15b-49ad6f54106f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2072, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002072__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2073-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2073, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2074, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2395", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2074", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ced48e16-dadf-405b-9460-e17aec089ad9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2073, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002073__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2074-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2074, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "179984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2075, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2396", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2075", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d0e5786-e1d3-4d45-be94-e1935405f9f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2074, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002074__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2075-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2075, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2076, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2397", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2076", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c76259b8-ce3a-4550-b3bb-5f10c22ab201", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2075, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002075__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2076-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2076, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2077, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2398", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2077", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98c0daf4-4380-4566-bed4-04e817c50e11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2076, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002076__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2077-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2077, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2078, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2399", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2078", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eba9dead-3fb7-4226-ae59-89217ebc2c8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2077, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002077__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2078-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2078, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2079, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2400", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2079", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89765631-7804-47ae-9247-c0cb09559dd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2078, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002078__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2079-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2079, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2080, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2401", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2080", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bae4f0e1-3b0f-4299-baac-e4f006b5d9b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2079, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002079__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2080-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2080, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2081, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2402", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2081", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ebc6bff-27b1-4e89-96b8-a58392690187", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2080, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002080__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2081-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2081, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2082, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2403", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2082", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b200945-5871-4c3d-9095-39d1e3f01c13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2081, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002081__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2082-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2082, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2083, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2404", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2083", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4c047ef-6707-429c-a5bc-1fe085f0fa7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2082, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002082__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2083-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2083, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2084, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2405", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2084", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d210c87-9733-4f34-b920-d357f6d2d1e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 180986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2083, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002083__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2084-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2084, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2085, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2406", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2085", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "35f26aa8-37e4-4ca6-a9a1-93781861b946", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2084, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002084__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2085-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2085, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "180937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2086, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2407", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2086", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "088b677e-0047-4886-a539-e70e75f1e965", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2085, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002085__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2086-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2086, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2087, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2408", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2087", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c526519-2bf4-43d0-acad-6e15ca3a31bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2086, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002086__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2087-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2087, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2088, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2409", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2088", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c04e5510-2f60-4f15-a9aa-8fb910c83f4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2087, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002087__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2088-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2088, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2089, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2410", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2089", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92b12f70-bff1-4508-b177-b5c2fa5d4674", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2088, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002088__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2089-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2089, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2090, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2411", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2090", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce775eed-9521-4ae9-a3a5-d00cb9e51527", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2089, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002089__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2090-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2090, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2091, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2412", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2091", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee08541b-4789-4940-af01-409225cd63fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2090, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002090__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2091-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2091, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2092, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2413", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2092", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5684165a-fec0-4ae6-8830-12eddde0abb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2091, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002091__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2092-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2092, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2093, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2414", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2093", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "affc6785-4e12-4a0f-90e9-261fc36c2a50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2092, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002092__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2093-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2093, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2094, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2415", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2094", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b46d69ad-b933-4596-8f31-6af23874b89c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2093, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002093__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2094-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2094, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2095, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2416", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2095", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d024440f-e9e4-4b1c-93f6-7fe6332f7bbe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 181940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2094, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002094__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2095-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2095, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2096, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2417", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2096", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee5304f9-a5be-4771-9aab-a8b2ad72d17d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2095, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002095__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2096-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2096, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2097, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2418", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2097", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32b01d5f-3992-4200-b06f-64c958817f4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2096, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002096__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2097-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2097, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "181984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2098, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2419", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2098", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "383b06e3-0cd6-4d36-8744-972b3afb0ebb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2097, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002097__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2098-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2098, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2099, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2420", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2099", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f277fe76-f833-4afc-bb1a-853b43658a4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2098, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002098__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2099-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2099, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2100, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2421", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2100", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0bce3e77-f705-4aec-b736-3aa533749707", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2099, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002099__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2100-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2100, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2101, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2422", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2101", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60477ab6-6728-41e5-9955-06fb119bc320", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2100, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002100__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2101-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2101, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2102, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2423", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2102", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d9016fc-d656-4c06-8401-7a63a373f544", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2101, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002101__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2102-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2102, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2103, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2424", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2103", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c074e526-5186-4392-8292-b8717cfc0004", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2102, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002102__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2103-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2103, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2104, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2425", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2104", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15d37637-668c-4973-85a2-83a04f8e268f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2103, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002103__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2104-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2104, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2105, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2426", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2105", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd26ecfd-01fb-486d-bb80-6c84a1052664", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2104, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002104__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2105-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2105, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2106, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2427", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2106", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6015a782-928f-4b91-a243-d64be5f8d104", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2105, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002105__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2106-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2106, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2107, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2428", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2107", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39f9875e-8685-42cb-8b2e-f22883376e20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 182986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2106, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002106__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2107-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2107, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2108, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2429", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2108", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ffde645e-02a7-426d-a3a7-fa3e29d290c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2107, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002107__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2108-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2108, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "182937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2109, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2430", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2109", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ca179d4-64a5-457b-91a4-7cef86fbe58c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2108, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002108__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2109-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2109, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2110, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2431", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2110", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66e16d0f-aafe-45d1-b571-f72b9c4d0229", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2109, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002109__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2110-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2110, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2111, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2432", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2111", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6d1f4d3-1c2e-4e9d-aa19-7efac6f7f3b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2110, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002110__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2111-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2111, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2112, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2433", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2112", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "012ad372-edf5-4777-bffa-8a4163f89644", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2111, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002111__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2112-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2112, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2113, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2434", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2113", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4be10ae-01f6-4fd1-bd77-f5999af093bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2112, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002112__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2113-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2113, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2114, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2435", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2114", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11817c6d-7f04-44dc-b05f-2abb1d3cdba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2113, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002113__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2114-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2114, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2115, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2436", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2115", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5cc9f4e-6dfb-4776-a5cf-3ddbb62626c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2114, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002114__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2115-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2115, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2116, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2437", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2116", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b031f6da-ceab-489c-8b4b-48c99b908ed0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2115, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002115__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2116-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2116, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2117, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2438", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2117", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01d3fc98-4ba5-4f93-9c7b-e93c5083cadb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2116, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002116__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2117-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2117, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2118, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2439", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2118", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9394390-b261-403b-9c55-0563a9a9a168", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 183940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2117, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002117__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2118-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2118, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2119, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2440", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2119", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc75acb5-fe4e-4737-8996-cb752ee90c68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2118, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002118__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2119-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2119, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2120, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2441", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2120", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd877bd4-f2df-404d-bdae-a1cb284a7b55", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2119, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002119__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2120-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2120, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "183984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2121, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2442", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2121", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d982f84-c14f-497a-8f9e-990e1b7dfd64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2120, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002120__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2121-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2121, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2122, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2443", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2122", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3e08557-0027-44d1-b8ee-758b0932672f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2121, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002121__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2122-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2122, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2123, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2444", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2123", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e96a1b5-eca4-429f-8be1-bbe0796f0dd7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2122, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002122__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2123-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2123, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2124, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2445", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2124", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe265bf4-adea-4283-9d3d-eedcea9bfd08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2123, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002123__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2124-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2124, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2125, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2446", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2125", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7711124e-275f-4dcc-a7f7-55eaf1957c1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2124, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002124__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2125-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2125, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2126, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2447", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2126", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55a09881-f65b-4873-8dc6-fe8fc512d8e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2125, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002125__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2126-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2126, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2127, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2448", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2127", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "810ae1da-9b49-404f-bc42-875ddee095ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2126, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002126__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2127-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2127, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2128, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2449", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2128", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e7e940e-6793-40bf-bdb9-3dd9304b0c4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2127, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002127__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2128-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2128, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2129, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2450", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2129", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21ae641b-75a4-4d74-b6da-a7c81c475caa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2128, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002128__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2129-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2129, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2130, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2451", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2130", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d74d8bac-ae79-448e-84ad-7c47701a64ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2129, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002129__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2130-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2130, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2131, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2452", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2131", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ed0845e-a916-4da4-a337-79d332a68492", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2130, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002130__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2131-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2131, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "184937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2132, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2453", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2132", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "feafd0fc-1f06-45ae-8844-21a4b3e27cde", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2131, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002131__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2132-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2132, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2133, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2454", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2133", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61a45086-f7cc-4d86-82f5-f1e11fe26ef6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2132, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002132__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2133-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2133, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2134, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2455", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2134", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "527c47e4-87ff-4a8b-a9dd-7578027cb5fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2133, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002133__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2134-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2134, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2135, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2456", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2135", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fdbd19e9-a172-4446-b300-afae370858a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2134, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002134__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2135-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2135, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2136, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2457", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2136", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf46f3ab-eebc-4fac-99e8-3a6c23d3da86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2135, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002135__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2136-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2136, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2137, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2458", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2137", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b04485db-a61a-402e-b831-3ca9304fad8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2136, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002136__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2137-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2137, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2138, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2459", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2138", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e5c42c8-83a6-4eca-a8e5-90f4ea6bb74f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2137, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002137__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2138-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2138, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2139, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2460", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2139", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a479a9af-8973-4a0a-a60a-717b9c1ee1a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2138, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002138__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2139-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2139, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2140, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2461", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2140", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7af6bce-9973-49f7-a909-828018b8e80c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2139, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002139__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2140-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2140, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2141, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2462", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2141", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b370073-3be8-48f6-9c50-a9ee2c183d8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 185940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2140, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002140__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2141-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2141, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2142, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2463", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2142", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14760c03-356f-4148-a8fc-c2e537922006", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2141, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002141__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2142-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2142, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2143, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2464", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2143", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c21d6ac7-94f6-4f64-9096-7d297a41d5fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2142, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002142__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2143-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2143, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "185968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2144, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2465", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2144", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "079c4dd0-1fd4-4b00-9705-c4508cffae63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2143, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002143__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2144-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2144, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2145, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2466", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2145", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abcb8a53-ff23-4c57-9ba7-71ad64130d07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2144, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002144__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2145-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2145, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2146, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2467", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2146", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b73c391-b001-401d-b866-21f0b4841023", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2145, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002145__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2146-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2146, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2147, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2468", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2147", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7646c3e-803c-45e6-b9dd-aa37637f25fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2146, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002146__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2147-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2147, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2148, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2469", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2148", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b10d1f7-a244-4b88-9e1f-bea92a31a46d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2147, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002147__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2148-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2148, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2149, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2470", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2149", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "559d368e-ac97-4198-a339-3e34cc408744", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2148, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002148__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2149-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2149, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2150, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2471", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2150", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21a6d454-26b8-4670-ba7f-71941facea27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2149, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002149__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2150-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2150, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2151, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2472", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2151", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a19ffae-2195-4cdc-ae3c-433012e3a872", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2150, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002150__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2151-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2151, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2152, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2473", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2152", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0284661-0608-4acb-afc2-4198e6119ebf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2151, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002151__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2152-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2152, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2153, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2474", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2153", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94f986f7-1caf-4c19-80bb-a9dae595381d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 186971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2152, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002152__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2153-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2153, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2154, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2475", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2154", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7af2dda7-a3ec-4aa2-abac-2ee03379255a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2153, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002153__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2154-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2154, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "186921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2155, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2476", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2155", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e2f5d44-0c32-4a9b-89ad-fd7ee17a6057", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2154, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002154__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2155-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2155, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2156, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2477", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2156", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43ec8e28-8ec6-469c-9182-fc3d1a0908da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2155, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002155__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2156-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2156, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2157, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2478", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2157", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63dbd258-f4c2-4ab6-a30f-24794cca7b30", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2156, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002156__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2157-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2157, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2158, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2479", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2158", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a98ea7db-285a-4176-8d9c-0149fb12c62a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2157, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002157__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2158-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2158, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2159, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2480", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2159", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "837fdd2b-d3a0-44b3-8134-75866782b543", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2158, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002158__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2159-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2159, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2160, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2481", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2160", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d5b9f28-e3db-4e9f-affe-02d226e1623d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2159, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002159__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2160-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2160, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2161, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2482", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2161", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26141ba4-9a81-4fc9-a6d9-5031555875fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2160, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002160__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2161-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2161, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2162, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2483", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2162", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0414f3e6-0f6b-4ca2-b648-f273dd3db8ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2161, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002161__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2162-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2162, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2163, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2484", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2163", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef2b07ed-b72e-4fd4-b046-3059625645ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2162, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002162__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2163-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2163, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2164, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2485", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2164", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcd6ff93-78c1-4a98-8b1b-9a3f7e338bd2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 187924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2163, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002163__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2164-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2164, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2165, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2486", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2165", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2206de2-f6d7-47f9-935f-96463b8b9827", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2164, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002164__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2165-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2165, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2166, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2487", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2166", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae3f98c9-5a62-4aab-b4ce-f0d5c3f72b3a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2165, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002165__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2166-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2166, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "187968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2167, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2488", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2167", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fa6338d-9cfe-48f8-a623-bdccfdddebef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2166, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002166__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2167-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2167, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2168, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2489", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2168", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0242462-bb60-40e1-94c4-eaf5672d970c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2167, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002167__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2168-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2168, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2169, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2490", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2169", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6653c4e-f567-47ce-aa4d-cecb74a934af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2168, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002168__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2169-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2169, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2170, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2491", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2170", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1073381-3dd6-4fda-bf5e-4e4e3581000b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2169, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002169__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2170-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2170, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2171, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2492", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2171", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f6a1004-e790-4e55-a5d6-85029b327853", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2170, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002170__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2171-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2171, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2172, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2493", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2172", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2ad3ee4-c798-4db5-ad9e-1f5ddb65cbb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2171, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002171__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2172-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2172, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2173, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2494", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2173", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd0ad211-e46c-43b9-80e6-3d94d5aa2cf1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2172, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002172__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2173-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2173, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2174, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2495", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2174", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6189cf1e-ab36-4600-8266-4935a844ab12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2173, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002173__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2174-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2174, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2175, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2496", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2175", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d8ed5b2-b7e1-4097-ad04-4dd8dde46905", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2174, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002174__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2175-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2175, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2176, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2497", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2176", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae189f95-2a79-428f-8a01-1321857017d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 188971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2175, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002175__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2176-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2176, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2177, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2498", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2177", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84a33b42-ab3e-42f5-b79c-a89db481edb2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2176, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002176__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2177-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2177, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "188921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2178, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2499", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2178", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed6f21dd-5269-4555-b104-e7adde84b313", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2177, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002177__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2178-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2178, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2179, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2500", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2179", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73da1f38-5d66-4264-8655-11c31c80292d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2178, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002178__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2179-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2179, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2180, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2501", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2180", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b928fec-6275-4f56-b8c0-05030aafbc00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2179, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002179__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2180-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2180, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2181, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2502", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2181", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a123e294-5663-481b-a503-198fdd3b4de5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2180, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002180__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2181-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2181, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2182, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2503", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2182", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8a38aae-64a1-47ab-9881-cdb1fb140c33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2181, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002181__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2182-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2182, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2183, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2504", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2183", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01c7faf2-a05b-4370-a3f9-56943c92c8d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2182, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002182__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2183-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2183, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2184, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2505", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2184", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c189f16-e883-4e69-a5e4-2d1723988a10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2183, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002183__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2184-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2184, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2185, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2506", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2185", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc6a7c13-6dce-463b-8cce-5f26d64ece34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2184, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002184__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2185-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2185, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2186, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2507", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2186", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23fe341e-86ce-455a-b3e6-871395dda27a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2185, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002185__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2186-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2186, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2187, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2508", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2187", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c5c9fa3-983c-47e4-998c-a498523ffd8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 189924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2186, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002186__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2187-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2187, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2188, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2509", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2188", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f6a2c39-8a53-452d-a863-1d101d8eb117", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2187, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002187__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2188-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2188, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2189, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2510", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2189", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf95ea7e-7954-41c0-a79a-54cec6f18901", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2188, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002188__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2189-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2189, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "189968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2190, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2511", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2190", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3acd91f9-c028-4aca-a6d5-31c6c86c688e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2189, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002189__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2190-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2190, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2191, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2512", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2191", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a5077ae-d345-4162-bc4d-84318013134d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2190, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002190__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2191-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2191, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2192, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2513", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2192", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3ad50bf-e2f1-4e3e-a4eb-cfc93f433264", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2191, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002191__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2192-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2192, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2193, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2514", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2193", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c106be2-6d1d-4c1f-b6ac-717595e37012", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2192, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002192__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2193-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2193, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2194, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2515", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2194", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e7999bb1-4e28-4855-a45d-510e9616c82a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2193, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002193__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2194-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2194, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2195, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2516", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2195", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9164497f-a118-44aa-a542-53ceaa71b81a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2194, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002194__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2195-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2195, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2196, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2517", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2196", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db76a5fb-fb6d-4216-b8a3-21fac14451a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2195, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002195__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2196-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2196, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2197, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2518", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2197", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd231aae-56dd-4e9c-a43e-88d824978660", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2196, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002196__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2197-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2197, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2198, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2519", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2198", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38453e63-75aa-484c-9332-e5f5316ff592", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2197, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002197__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2198-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2198, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2199, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2520", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2199", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c64151a-38f9-463e-8c79-121f3ce6ae6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 190971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2198, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002198__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2199-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2199, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2200, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2521", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2200", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ed9ceae-fc5f-42c5-8cf1-8f137ca14d60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2199, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002199__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2200-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2200, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "190921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2201, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2522", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2201", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "faf4d578-f469-4bf8-9f0c-378284f03ef6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2200, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002200__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2201-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2201, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2202, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2523", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2202", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "749cf2d0-36f1-42c4-a4c5-a1329d7cd620", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2201, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002201__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2202-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2202, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2203, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2524", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2203", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed756405-910b-4f1c-9780-d43060323611", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2202, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002202__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2203-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2203, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2204, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2525", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2204", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba3b9a54-b04d-4b77-bdd8-0efa2bd3f9f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2203, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002203__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2204-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2204, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2205, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2526", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2205", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a81e69d1-652c-4ddd-aae5-c15aea891d92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2204, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002204__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2205-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2205, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2206, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2527", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2206", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4db9ede-c9ad-43fc-ba4b-a5c6897c5673", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2205, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002205__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2206-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2206, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2207, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2528", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2207", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "100b0d98-d2b3-439f-b553-f3524a5a40ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2206, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002206__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2207-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2207, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2208, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2529", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2208", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "863cbae8-b2cb-434d-b1df-995694e5299e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2207, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002207__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2208-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2208, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2209, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2530", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2209", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d96d5c50-1cb2-4e20-bd04-f6809fa4fc59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2208, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002208__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2209-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2209, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2210, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2531", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2210", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd40c833-5331-4a6a-bf1c-fbaf8b2a1bc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 191924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2209, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002209__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2210-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2210, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2211, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2532", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2211", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ccfe2cf-41ba-420b-b826-1f88afc4efa3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2210, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002210__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2211-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2211, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2212, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2533", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2212", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d3b9485-b805-463f-a41c-4c3e5041edcf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2211, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002211__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2212-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2212, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "191953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2213, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2534", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2213", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5759d658-2852-41e3-87c2-6f4754e31047", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2212, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002212__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2213-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2213, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2214, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2535", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2214", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76e47d86-3cc7-4aa6-8d69-d2d8aedb728c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2213, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002213__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2214-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2214, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2215, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2536", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2215", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6faefa59-8da9-4fc4-8a8f-b72ad59a2658", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2214, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002214__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2215-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2215, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2216, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2537", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2216", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "628f3cde-bbfb-4a5f-9d2b-c9a0e03b14a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2215, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002215__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2216-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2216, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2217, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2538", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2217", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d53beec-b4a6-46bd-b574-f310f81af283", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2216, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002216__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2217-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2217, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2218, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2539", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2218", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "caf77e21-6f83-40e3-8a45-a8126ce22c44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2217, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002217__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2218-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2218, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2219, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2540", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2219", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "662e1012-6711-4dc6-af8f-e4fd6fd1a60f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2218, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002218__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2219-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2219, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2220, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2541", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2220", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fe12bd7-a3e5-4c27-9b42-67828535228b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2219, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002219__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2220-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2220, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2221, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2542", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2221", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7cc0cab0-480b-4425-83c9-40beaaf459f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2220, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002220__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2221-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2221, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2222, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2543", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2222", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99f0c9c3-fd59-4a39-bc78-4d319962364d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 192955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2221, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002221__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2222-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2222, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2223, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2544", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2223", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c00785b5-7869-482a-b5fc-b4d3b0b7d095", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2222, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002222__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2223-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2223, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "192906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2224, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2545", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2224", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aff20fda-1e35-44f5-8486-4b1ddca5aef4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2223, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002223__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2224-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2224, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2225, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2546", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2225", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3150524-dd6b-4a6a-989b-f008c9eeb76a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2224, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002224__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2225-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2225, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2226, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2547", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2226", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cf362d1-c096-45df-be78-abed6124520a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2225, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002225__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2226-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2226, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2227, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2548", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2227", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7409d888-1c73-4ca4-94d0-acb56c3d7d39", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2226, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002226__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2227-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2227, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2228, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2549", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2228", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef05557c-44fb-4794-9b2d-1c49f016c190", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2227, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002227__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2228-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2228, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2229, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2550", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2229", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f30ee7d4-8f8c-4055-82b6-f28f0037f575", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2228, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002228__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2229-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2229, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2230, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2551", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2230", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "342ce564-ea28-4a79-ab66-847384638d1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2229, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002229__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2230-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2230, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2231, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2552", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2231", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67a2480a-7def-44ca-aa6e-729d5603b38f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2230, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002230__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2231-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2231, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2232, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2553", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2232", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b8d97ef-2f50-4cf5-a4bc-64c0adfa3222", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2231, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002231__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2232-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2232, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2233, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2554", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2233", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "027c25fd-6a80-48de-88b8-29a42f9b9ea5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 193908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2232, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002232__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2233-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2233, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2234, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2555", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2234", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cfc2356-bf95-45be-9685-cb398a780305", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2233, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002233__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2234-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2234, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2235, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2556", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2235", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0df3b293-7867-4fc7-b9d2-87d7be2f758c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2234, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002234__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2235-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2235, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "193953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2236, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2557", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2236", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2337c85-4398-4905-bad8-f99616769d68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2235, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002235__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2236-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2236, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2237, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:21:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2558", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2237", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d4eef98-8a1e-475b-9d81-a6a30c20fb59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2236, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002236__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2237-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2237, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2238, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2559", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2238", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0467b44-5cd8-46cb-89f8-4b3f9e038222", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2237, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002237__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2238-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2238, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2239, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2560", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2239", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de920183-db0e-48cf-9078-e887da5f24c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2238, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002238__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2239-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2239, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2240, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2561", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2240", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "786b5b6a-7a93-4d26-810c-ba676aea2128", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2239, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002239__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2240-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2240, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2241, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2562", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2241", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9508b34-d243-4df5-85b6-1943cf102abf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2240, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002240__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2241-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2241, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2242, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2563", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2242", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9adcae4-f38d-4ceb-97ea-08fbe866bcfd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2241, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002241__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2242-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2242, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2243, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2564", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2243", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58780ee6-d3f2-4e5c-9923-db6c15d6cbdf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2242, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002242__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2243-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2243, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2244, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2565", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2244", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43a6226b-8730-4665-ad4f-81cdcbbe7781", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2243, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002243__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2244-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2244, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2245, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2566", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2245", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9efd8e68-3630-4383-844b-93937e11c2a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 194955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2244, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002244__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2245-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2245, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2246, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2567", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2246", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e7915d3-dc45-4943-866c-07a318dea724", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2245, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002245__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2246-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2246, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "194906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2247, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2568", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2247", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebb2269a-63aa-4120-9f3b-1de23aadd927", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2246, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002246__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2247-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2247, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2248, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2569", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2248", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e60d8919-8572-4135-aaa3-8bc8bc93a75b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2247, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002247__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2248-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2248, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2249, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2570", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2249", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31fbb8fb-2a39-4aaf-bd82-8cadacfe1758", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2248, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002248__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2249-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2249, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2250, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2571", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2250", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f08e4cfb-cc86-4896-8213-438d877587f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2249, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002249__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2250-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2250, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2251, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2572", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2251", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2d4491b-3327-47da-8ba6-343d84c6ac92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2250, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002250__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2251-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2251, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2252, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2573", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2252", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7945a70b-1870-443f-a9d6-d3e6b45da3d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2251, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002251__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2252-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2252, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2253, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2574", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2253", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12960387-43d6-4229-8462-623da6d09b5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2252, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002252__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2253-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2253, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2254, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2575", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2254", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e43e02e-1aa3-49ec-bc63-58664d4d611a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2253, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002253__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2254-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2254, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2255, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2576", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2255", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "278c85ae-b658-4857-bab5-5b5d0bbae2e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2254, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002254__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2255-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2255, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2256, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2577", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2256", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4834d9b3-65a0-4260-af04-868c06fdc782", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 195908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2255, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002255__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2256-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2256, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2257, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2578", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2257", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96e42859-2d1e-415b-ac13-48d855610838", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2256, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002256__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2257-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2257, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2258, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2579", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2258", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72ada411-8032-4562-9a6a-413444f24840", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2257, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002257__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2258-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2258, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "195953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2259, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2580", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2259", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aef2b70e-8ecc-48c7-ad97-b4524dc69434", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2258, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002258__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2259-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2259, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2260, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2581", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2260", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "643a5d13-9b0a-4060-90a9-dadee20e2252", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2259, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002259__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2260-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2260, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2261, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2582", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2261", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12a00325-59d0-43ca-a399-cf5fc25a3400", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2260, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002260__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2261-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2261, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2262, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2583", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2262", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6d19ac5-8962-4dc6-98a8-603d55ab1e93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2261, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002261__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2262-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2262, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2263, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2584", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2263", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39061117-972e-4b9a-a585-ae2840b42a87", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2262, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002262__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2263-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2263, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2264, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2585", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2264", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30b5049e-6baf-401e-aec0-8431cd0c483a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2263, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002263__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2264-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2264, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2265, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2586", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2265", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bacb8e0-ec1e-4cf3-bbcf-9a15312611ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2264, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002264__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2265-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2265, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2266, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2587", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2266", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa324061-ec36-4245-9416-f2bda1371817", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2265, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002265__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2266-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2266, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2267, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2588", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2267", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46d2e318-6790-4606-9f9d-4f1d472e6fad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2266, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002266__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2267-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2267, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2268, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2589", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2268", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16c6c7d9-9694-4849-8e4d-ca5dd2da1451", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 196955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2267, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002267__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2268-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2268, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2269, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2590", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2269", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70e247fe-eef4-48d4-802f-9dd81ea53e54", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2268, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002268__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2269-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2269, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2270, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2591", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2270", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecd3300e-b0d8-44f9-aba7-d566278eac82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2269, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002269__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2270-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2270, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "196984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2271, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2592", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2271", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c66941f-c7f8-44f8-9430-07fd9e5bc94f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2270, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002270__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2271-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2271, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2272, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2593", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2272", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac877a5f-21c0-4281-a0ba-e15365e58917", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2271, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002271__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2272-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2272, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2273, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2594", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2273", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2becb5dc-9acc-45eb-a1ab-4de5be32d64d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2272, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002272__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2273-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2273, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2274, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2595", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2274", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e80bb5a-9b82-481b-bdc7-cb5887f36124", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2273, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002273__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2274-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2274, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2275, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2596", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2275", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f1b12de-cb6f-4be4-a241-f7b805eda709", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2274, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002274__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2275-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2275, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2276, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2597", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2276", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6500f645-b2a2-4e67-8219-d13be4204522", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2275, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002275__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2276-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2276, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2277, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2598", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2277", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a48746e7-b1a8-49ae-8f92-98bd0f90cc17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2276, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002276__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2277-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2277, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2278, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2599", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2278", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4b1acf0-fcf7-4ff6-9f4b-2dcce01b2569", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2277, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002277__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2278-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2278, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2279, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2600", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2279", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c1d27ef-4f8b-42f9-963e-820dc183445c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2278, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002278__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2279-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2279, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2280, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2601", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2280", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4aa2469b-3ffd-4f28-9ddb-0ae3dd0a8f37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 197986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2279, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002279__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2280-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2280, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2281, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2602", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2281", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83175cbf-960f-4287-af99-3b531d317ed6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2280, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002280__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2281-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2281, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "197937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2282, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2603", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2282", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc36fdec-b856-42a8-9422-91f4c9448cbe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2281, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002281__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2282-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2282, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2283, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2604", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2283", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8788ad07-dd86-43e9-8f5b-b8a5bc4e606d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2282, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002282__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2283-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2283, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2284, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2605", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2284", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b00e6a6e-9e66-496e-96e7-43793a84597e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2283, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002283__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2284-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2284, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2285, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2606", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2285", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0524d61f-8f99-453f-902e-2ed984562f79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2284, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002284__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2285-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2285, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2286, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2607", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2286", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c940cbe5-9561-4859-8889-c095ee3c0cc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2285, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002285__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2286-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2286, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2287, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2608", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2287", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5909696-4969-41ba-abc0-27be4b0a35df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2286, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002286__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2287-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2287, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2288, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2609", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2288", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "654674a1-42f6-48da-9003-f5844ea2191b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2287, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002287__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2288-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2288, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2289, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2610", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2289", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "325e4643-0f7f-4dfd-be4d-bb617a32c7e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2288, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002288__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2289-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2289, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2290, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2611", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2290", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da730968-2f9f-437f-8565-db9b07a9a9ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2289, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002289__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2290-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2290, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2291, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2612", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2291", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3dd74a63-cfe0-4230-94cc-ad8321db50fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 198940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2290, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002290__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2291-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2291, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2292, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2613", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2292", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0254e594-5abf-4fce-af1d-cf9534dc0971", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2291, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002291__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2292-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2292, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2293, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2614", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2293", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a91e57b-90cf-4815-bfed-eda1c707b616", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2292, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002292__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2293-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2293, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "198984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2294, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2615", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2294", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "854433f6-e9e3-4240-b2ef-ffa198320376", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2293, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002293__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2294-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2294, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2295, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2616", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2295", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "35d51f3e-c81d-48ae-887d-fb9c56ed338e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2294, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002294__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2295-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2295, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2296, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2617", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2296", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "164c116c-e5eb-49da-98b2-b666f8a01ee9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2295, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002295__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2296-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2296, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2297, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2618", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2297", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de7d1958-f73b-4b48-98fe-7a1d612af678", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2296, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002296__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2297-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2297, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2298, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2619", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2298", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa04e9db-f438-410d-9b52-61249866ecc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2297, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002297__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2298-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2298, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2299, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2620", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2299", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6a3b697-3d08-4af5-85f2-c61ab4592f18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2298, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002298__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2299-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2299, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2300, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2621", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2300", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "559f26f6-eb2b-4388-9fa8-c79f3dfaf08c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2299, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002299__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2300-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2300, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2301, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2622", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2301", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3ecccd4-c7da-40fc-b12e-56a0da218aa2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2300, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002300__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2301-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2301, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2302, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2623", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2302", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75e6ed57-fe0f-4fd0-8702-735ef39e6790", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2301, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002301__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2302-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2302, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2303, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2624", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2303", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef6a54d1-96a3-492d-880d-7f8ebd31b831", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 199986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2302, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002302__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2303-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2303, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2304, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2625", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2304", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "487544ec-fdce-4cc4-8377-8109d1fdc615", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2303, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002303__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2304-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2304, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "199937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2305, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2626", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2305", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73c71fb9-57cc-403a-9d69-78e7273fa78e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2304, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002304__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2305-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2305, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2306, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2627", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2306", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc9f98f6-3d95-41d0-b3e5-8059ba71fd62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2305, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002305__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2306-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2306, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2307, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2628", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2307", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "570fd181-5529-414e-b700-78769e1eb9fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2306, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002306__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2307-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2307, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2308, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2629", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2308", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5da0bdf-6299-4c79-b275-63cedccd1de8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2307, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002307__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2308-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2308, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2309, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2630", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2309", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1751a330-8bf2-45e3-a343-6c2f8152b5c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2308, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002308__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2309-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2309, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2310, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2631", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2310", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c6a5d44-e1b2-4925-adb3-ea65ba7211ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2309, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002309__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2310-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2310, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2311, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2632", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2311", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45cbc740-f681-4184-b3bf-6e63b388d0ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2310, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002310__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2311-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2311, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2312, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2633", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2312", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb73ef0f-1c12-43dc-b1ba-4ceb6401ce03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2311, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002311__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2312-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2312, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2313, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2634", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2313", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c11d3ab5-cf5a-44ca-aae8-6b27b95b76e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2312, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002312__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2313-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2313, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2314, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2635", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2314", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bd2a05c-ed3c-4fe5-a686-697a94e86294", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 200940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2313, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002313__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2314-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2314, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2315, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2636", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2315", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8601cf5c-3c5e-4fa3-96ab-8580a742f792", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2314, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002314__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2315-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2315, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2316, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2637", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2316", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d72c06f-6e15-4684-999a-197db3ddc97b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2315, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002315__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2316-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2316, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "200984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2317, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2638", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2317", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1cf3aeba-5a63-4c78-b374-3be2e6b5328e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2316, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002316__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2317-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2317, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2318, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2639", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2318", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50bfdc84-cf85-4c91-8e81-a91d3c396c57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2317, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002317__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2318-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2318, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2319, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2640", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2319", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28a4bd62-3f78-4332-a177-7ffc1c71ff41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2318, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002318__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2319-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2319, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2320, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2641", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2320", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9999fcdb-e9a9-4778-9be3-0d5c9cabd2a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2319, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002319__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2320-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2320, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2321, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2642", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2321", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e55a2e0b-fbd4-442c-9604-082b7226d4ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2320, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002320__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2321-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2321, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2322, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2643", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2322", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c11af4b-695c-418a-9acf-7e204c343d16", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2321, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002321__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2322-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2322, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2323, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2644", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2323", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dba5a187-ffd5-4015-a0d6-087b8cf2cbcd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2322, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002322__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2323-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2323, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2324, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2645", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2324", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd5c35bf-6cc7-41b1-858c-c9958129e3f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2323, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002323__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2324-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2324, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2325, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2646", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2325", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "650275be-9cf6-4522-8928-dd98c526c355", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2324, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002324__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2325-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2325, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2326, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2647", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2326", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "801920b2-77bb-4570-af4d-1ac8a744494b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 201986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2325, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002325__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2326-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2326, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2327, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2648", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2327", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "510910a5-6461-4850-ad3a-b7954c28114d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2326, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002326__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2327-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2327, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "201937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2328, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2649", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2328", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de97d12d-367b-4eb9-8fa3-8ce8b042f4ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2327, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002327__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2328-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2328, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2329, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2650", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2329", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5552f3ce-0707-400f-884b-5035c4fa0081", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2328, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002328__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2329-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2329, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2330, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2651", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2330", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ce90c6b-3d8b-49ea-8651-08de075e458d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2329, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002329__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2330-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2330, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2331, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2652", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2331", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c78bbf3a-1433-4567-890a-f258199d862e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2330, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002330__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2331-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2331, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2332, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2653", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2332", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abcf8818-1f16-4726-813a-451ae333bc83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2331, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002331__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2332-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2332, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2333, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2654", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2333", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "faa324fe-7f65-4df8-bb3c-41b71627f010", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2332, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002332__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2333-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2333, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2334, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2655", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2334", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c4326ab-6fca-42a6-b869-13033428ddd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2333, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002333__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2334-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2334, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2335, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2656", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2335", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "296082be-cc18-401d-a2c3-e5cca8513294", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2334, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002334__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2335-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2335, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2336, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2657", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2336", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "317882a1-05ac-4c4a-aa8b-03df699402ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2335, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002335__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2336-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2336, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2337, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2658", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2337", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5fc9b01-29f4-409f-983a-f6a3603f56af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 202940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2336, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002336__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2337-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2337, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2338, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2659", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2338", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2487b0b-121b-451c-b385-36e124880daf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2337, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002337__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2338-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2338, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2339, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2660", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2339", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "93a7df2f-828c-44f7-81d1-27ffad6a7af1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2338, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002338__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2339-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2339, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "202968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2340, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2661", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2340", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ac8fc36-4915-4010-adb9-95470bb50641", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2339, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002339__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2340-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2340, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2341, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2662", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2341", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f52e6c45-54de-4c85-90e2-9e9f15ecdddd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2340, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002340__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2341-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2341, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2342, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2663", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2342", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7cf074d7-d26a-487e-8116-9c08559f345f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2341, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002341__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2342-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2342, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2343, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2664", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2343", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88317e95-37a2-42ad-8268-40bc6a3df31d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2342, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002342__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2343-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2343, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2344, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2665", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2344", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5afeaaf4-4061-4476-b19c-71c0bce40832", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2343, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002343__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2344-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2344, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2345, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2666", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2345", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dfec7d89-e468-4faa-93ad-594fcacf66d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2344, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002344__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2345-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2345, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2346, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2667", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2346", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6ad01ce-7eba-4a64-85b6-1723596385ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2345, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002345__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2346-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2346, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2347, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2668", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2347", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d977bfd2-77c4-4649-b117-daf02c2b65fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2346, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002346__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2347-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2347, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2348, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2669", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2348", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76231212-6758-45a9-8a58-571dd9937780", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2347, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002347__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2348-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2348, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2349, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2670", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2349", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ffcfdc40-38af-4a1b-90f1-d45133111d68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 203971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2348, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002348__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2349-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2349, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2350, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2671", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2350", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a74f8e0-e689-40a2-86a2-7d31586ed814", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2349, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002349__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2350-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2350, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "203921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2351, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2672", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2351", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d753de7-6c7b-4361-a0ed-7ddbab702a64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2350, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002350__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2351-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2351, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2352, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2673", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2352", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e82204e-d4b5-4c8a-8e01-514fab87d909", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2351, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002351__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2352-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2352, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2353, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2674", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2353", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "457f394a-312f-4084-8aa1-cddeb3f4ba20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2352, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002352__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2353-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2353, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2354, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2675", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2354", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d4926d0-45b0-4670-b180-ec84fef0c07c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2353, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002353__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2354-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2354, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2355, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2676", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2355", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15e2bb66-b79e-40eb-aadb-24ec4dd3b525", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2354, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002354__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2355-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2355, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2356, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2677", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2356", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12691883-04ea-4006-8d7f-b6cdf7aa7955", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2355, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002355__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2356-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2356, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2357, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2678", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2357", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1985c788-8629-470f-aad3-5c70516d69a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2356, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002356__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2357-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2357, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2358, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2679", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2358", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "334b08e1-8e21-4ca3-ba10-1e341454f55b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2357, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002357__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2358-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2358, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2359, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2680", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2359", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe791544-cfb8-4bb1-90f1-7c388a36d431", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2358, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002358__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2359-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2359, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2360, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2681", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2360", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6407519-e91b-4e58-97d9-a2c152247a34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 204924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2359, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002359__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2360-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2360, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2361, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2682", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2361", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff4899be-7b7e-4223-b48c-aa6e34eba8a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2360, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002360__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2361-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2361, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2362, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2683", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2362", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d278abd9-cfd0-4ef5-829d-f422081ec4de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2361, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002361__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2362-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2362, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "204968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2363, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2684", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2363", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "144845c4-e525-4ad7-9cdd-8d932d2d42d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2362, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002362__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2363-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2363, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2364, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2685", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2364", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6066542-511e-44dd-9678-fc3398c0751c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2363, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002363__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2364-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2364, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2365, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2686", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2365", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0c016de-75e0-4639-9880-b8141d093899", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2364, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002364__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2365-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2365, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2366, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2687", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2366", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b12fd8d3-1d39-492a-bcdc-6267c24dda10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2365, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002365__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2366-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2366, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2367, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2688", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2367", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e88accc3-eff6-4f1d-b500-3ea70a251659", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2366, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002366__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2367-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2367, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2368, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2689", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2368", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "744f33d9-554a-4c7e-969b-91b5705d6804", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2367, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002367__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2368-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2368, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2369, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2690", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2369", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23bac9b4-2da3-480d-9b8d-b2d5bb86cea9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2368, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002368__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2369-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2369, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2370, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2691", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2370", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b916de0-6731-45b7-97e2-ede0b9a2f380", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2369, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002369__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2370-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2370, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2371, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2692", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2371", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13d61637-b13a-468d-a7cf-41784bc52ab8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2370, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002370__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2371-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2371, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2372, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2693", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2372", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7f83d1a-37fe-4d89-8903-ce707657ea70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 205971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2371, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002371__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2372-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2372, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2373, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2694", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2373", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54e05adb-eb5a-498f-a3db-eda59b2905cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2372, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002372__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2373-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2373, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "205921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2374, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2695", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2374", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a1893e2-46dd-4229-8a7c-434f0b59ee59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2373, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002373__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2374-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2374, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2375, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2696", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2375", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1be2817e-cc65-47f9-aa55-ba5f357e1516", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2374, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002374__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2375-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2375, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2376, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2697", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2376", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99b2c23a-c980-4ff6-9c26-b0a93b99c1d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2375, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002375__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2376-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2376, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2377, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2698", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2377", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f15b8527-2a26-4d9e-b2e0-db45cba0f6ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2376, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002376__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2377-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2377, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2378, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2699", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2378", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33e6a43f-11cf-4da3-9ae1-7dd34e114b24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2377, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002377__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2378-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2378, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2379, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2700", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2379", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ed9cc96-925d-4070-ba11-0d02d87a73df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2378, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002378__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2379-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2379, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2380, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2701", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2380", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2ffdf5e-b3df-4e21-a3f8-1592e49b6b45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2379, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002379__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2380-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2380, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2381, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2702", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2381", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28b85f4d-7d0b-4094-8ae0-6114347d085d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2380, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002380__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2381-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2381, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2382, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2703", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2382", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1bdf81b-630c-4594-8f01-7846ac355f52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2381, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002381__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2382-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2382, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2383, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2704", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2383", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "000045b4-cc9a-40c1-9958-304f595f1008", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 206924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2382, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002382__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2383-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2383, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2384, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2705", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2384", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac810343-112a-4c8d-9d9f-355f7be0c9f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2383, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002383__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2384-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2384, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2385, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2706", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2385", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc9aa1ad-337d-4e8c-a5c1-e09f1d27a105", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2384, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002384__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2385-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2385, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "206968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2386, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2707", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2386", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d962226-bfb7-401d-920a-646e2f5b6c34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2385, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002385__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2386-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2386, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2387, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2708", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2387", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5aebd3b-ee68-4e3c-9279-1098bf7c2593", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2386, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002386__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2387-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2387, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2388, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2709", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2388", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f63949f-ce6b-4e25-be82-8e82513615a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2387, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002387__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2388-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2388, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2389, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2710", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2389", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30b76990-34be-4200-9e8d-8f6feda20f10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2388, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002388__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2389-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2389, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2390, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2711", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2390", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8642f5b-99a4-4fdc-9405-4e8cff822fbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2389, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002389__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2390-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2390, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2391, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2712", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2391", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4385846-dbe9-4879-8b25-82ea5172f6dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2390, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002390__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2391-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2391, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2392, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2713", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2392", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3703abe4-e5d9-48aa-affe-30358d18c1c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2391, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002391__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2392-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2392, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2393, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2714", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2393", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a16c7245-0692-47c3-bf4b-b3dc7cc00b85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2392, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002392__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2393-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2393, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2394, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2715", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2394", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91d40937-8c44-413f-9f4e-e446a23ec9f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2393, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002393__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2394-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2394, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2395, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2716", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2395", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5f8cdcf-b4b2-4462-8d3f-894bc6147669", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 207971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2394, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002394__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2395-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2395, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2396, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2717", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2396", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c70e1ac1-4cce-4da5-94e0-ad9444ecf7fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2395, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002395__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2396-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2396, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "207921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2397, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2718", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2397", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10312cd2-55c4-4f20-9610-509ebea08412", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2396, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002396__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2397-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2397, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2398, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2719", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2398", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81a2ae6a-a8a3-49bf-8562-2f17e1e8e384", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2397, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002397__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2398-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2398, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2399, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2720", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2399", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03eb9625-6fdc-49a6-950a-abb960b66b93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2398, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002398__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2399-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2399, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2400, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2721", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2400", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b61a8560-55d7-44c3-8f8f-5964be7824dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2399, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002399__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2400-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2400, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2401, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2722", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2401", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1e3903c-4acf-4342-bd4c-338ce47dcc75", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2400, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002400__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2401-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2401, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2402, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2723", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2402", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b9a9d36-a615-4f1b-991e-5959406804d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2401, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002401__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2402-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2402, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2403, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2724", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2403", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2496eea7-4dd8-427b-aa2c-957d4f76d14b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2402, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002402__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2403-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2403, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2404, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2725", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2404", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bf95ec2-89b5-4f99-a8b8-c187cd25a7a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2403, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002403__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2404-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2404, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2405, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2726", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2405", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f1b8445-63d6-46ca-af00-f884166e12d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2404, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002404__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2405-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2405, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2406, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2727", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2406", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96f89761-3daf-4096-9c9b-d2d5610c2272", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 208924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2405, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002405__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2406-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2406, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2407, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2728", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2407", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5262e44-5021-4ac9-94f7-0da1f29972f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2406, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002406__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2407-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2407, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2408, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2729", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2408", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fafa8818-2283-4242-9843-172af7d03991", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2407, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002407__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2408-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2408, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "208953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2409, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2730", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2409", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6dd38090-13c5-49af-a990-412a19833f7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2408, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002408__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2409-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2409, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2410, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2731", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2410", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b4585c7-7ea7-4e6a-aafc-6fd1c76e2bcf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2409, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002409__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2410-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2410, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2411, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2732", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2411", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "409e5efb-a71c-4eb6-a32d-61e31744b97b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2410, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002410__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2411-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2411, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2412, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2733", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2412", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a83fe4c-b60b-4528-9691-bc275abc3acc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2411, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002411__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2412-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2412, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2413, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2734", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2413", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85545be4-b94d-4917-9777-8b8ef640b145", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2412, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002412__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2413-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2413, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2414, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2735", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2414", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9daf674e-d52a-4741-8a92-16416e697119", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2413, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002413__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2414-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2414, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2415, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2736", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2415", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf9a9a9b-f0d1-40a1-90ce-524272a3399d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2414, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002414__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2415-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2415, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2416, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2737", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2416", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "593bd1ae-c127-4f53-b282-c59a026153cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2415, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002415__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2416-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2416, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2417, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2738", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2417", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca3f8845-8a09-4c6f-8cea-81ab5a33add9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2416, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002416__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2417-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2417, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2418, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2739", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2418", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03f82b3e-5692-4115-8fdf-ec30b2b45245", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 209955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2417, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002417__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2418-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2418, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2419, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2740", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2419", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2c749ce-23ff-4bd8-81ed-a82030eb974e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2418, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002418__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2419-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2419, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "209906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2420, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2741", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2420", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16d9c7a4-55b9-4311-8a14-8ae41b619f60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2419, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002419__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2420-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2420, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2421, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2742", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2421", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e51cfb75-46be-4b57-ab1d-239e96a2df8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2420, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002420__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2421-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2421, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2422, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2743", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2422", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "999246a2-7a63-42bb-a002-e6c0583e4cdd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2421, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002421__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2422-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2422, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2423, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2744", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2423", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42d3f025-8be4-491e-a183-8b8e4d7ee989", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2422, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002422__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2423-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2423, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2424, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2745", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2424", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01b79141-077c-444e-a615-7c2f93d6b7f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2423, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002423__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2424-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2424, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2425, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2746", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2425", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a92efc0-c16d-4746-89dd-37bc48a9ffdc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2424, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002424__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2425-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2425, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2426, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2747", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2426", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5075957-cd57-4966-bd53-b8d0277e4389", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2425, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002425__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2426-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2426, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2427, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2748", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2427", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e05594f4-5aa5-45d1-a963-49cf276fce4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2426, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002426__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2427-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2427, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2428, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2749", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2428", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "064fcdca-3f63-4b2a-bb69-8992037e33d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2427, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002427__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2428-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2428, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2429, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2750", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2429", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cef977f7-52ee-41d0-b7a2-0f1b737694c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 210908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2428, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002428__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2429-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2429, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2430, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2751", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2430", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e624243d-fb5a-4ad4-92d8-5da6227a01d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2429, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002429__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2430-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2430, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2431, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2752", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2431", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86b7e55f-b204-448b-a821-13ec8e2d1d48", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2430, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002430__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2431-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2431, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "210953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2432, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2753", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2432", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d75aef8c-15f5-42d8-9dc1-ceb5561dba57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2431, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002431__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2432-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2432, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2433, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2754", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2433", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4528f90f-c013-42ff-bbd8-e998228b8d1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2432, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002432__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2433-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2433, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2434, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2755", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2434", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc0ecb33-0948-423c-b5f1-8b9197a2f499", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2433, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002433__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2434-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2434, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2435, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2756", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2435", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aff7fe55-c106-4154-b2df-9c3e27f5a4b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2434, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002434__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2435-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2435, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2436, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2757", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2436", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ed6274b-a533-4f5b-be47-dc6956644b68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2435, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002435__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2436-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2436, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2437, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2758", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2437", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d046fec9-7a8e-42d1-ada1-ed0ea3ffcdc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2436, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002436__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2437-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2437, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2438, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2759", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2438", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a203577-a8a3-4610-9b8a-afaaa89899ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2437, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002437__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2438-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2438, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2439, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2760", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2439", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70d3bd99-8337-482c-bf4b-5b9635f2c2a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2438, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002438__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2439-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2439, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2440, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2761", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2440", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bedc904-cdc5-4279-8469-77fa3458cc07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2439, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002439__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2440-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2440, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2441, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2762", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2441", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ffdd303b-0216-4348-aabb-71de043535e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 211955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2440, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002440__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2441-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2441, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2442, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2763", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2442", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "373cd9bc-9d92-4dc3-90fb-ce52477a70d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2441, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002441__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2442-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2442, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "211906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2443, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2764", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2443", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cdf419e6-99f0-4071-815e-9a3b0c41e53d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2442, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002442__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2443-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2443, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2444, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2765", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2444", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c23fad4f-2961-43c5-88ab-1b6a3d54d639", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2443, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002443__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2444-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2444, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2445, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2766", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2445", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4da6e833-0b85-498b-a32f-0ff9a20c29b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2444, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002444__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2445-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2445, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2446, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2767", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2446", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b52516ee-6075-418b-a950-291a54524523", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2445, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002445__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2446-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2446, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2447, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2768", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2447", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b93524b-652b-4699-8e95-0e722e62ec0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2446, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002446__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2447-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2447, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2448, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2769", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2448", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e8134c5-4160-4c70-bf88-4b621a5565bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2447, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002447__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2448-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2448, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2449, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2770", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2449", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd7d9cc5-4570-47ac-924f-679b3f988bfe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2448, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002448__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2449-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2449, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2450, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2771", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2450", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4080766c-7bcd-4512-8a2e-58fb165efa33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2449, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002449__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2450-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2450, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2451, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2772", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2451", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9ab36cf-b27e-43ef-b8b7-6890b1f8448c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2450, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002450__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2451-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2451, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2452, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2773", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2452", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dec6277a-7a76-4105-9240-a32378485b34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 212908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2451, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002451__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2452-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2452, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2453, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2774", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2453", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "773a1adf-dc43-4bd2-afbb-1a78395febec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2452, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002452__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2453-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2453, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2454, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2775", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2454", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63febcdb-dd9b-44db-86bf-8e7d26a5115c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2453, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002453__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2454-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2454, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "212953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2455, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2776", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2455", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "778066e2-ddd5-4d6c-932d-215cb1e984be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2454, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002454__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2455-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2455, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2456, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2777", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2456", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "813d2ce0-edbd-4b23-bdaf-35a154f8adb1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2455, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002455__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2456-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2456, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2457, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2778", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2457", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80739b8e-bf8f-4056-b427-e680129540ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2456, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002456__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2457-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2457, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2458, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2779", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2458", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a78183b2-ac68-4e3b-9b60-4a9251727b27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2457, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002457__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2458-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2458, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2459, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2780", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2459", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2157ab9-c1a9-470c-8bbd-89aec78120d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2458, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002458__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2459-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2459, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2460, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2781", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2460", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1846df1-abb2-4695-aea8-d415f3962420", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2459, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002459__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2460-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2460, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2461, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2782", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2461", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b227e2dd-1c4f-49a7-bf60-62d418bbde5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2460, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002460__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2461-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2461, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2462, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2783", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2462", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "861e3bdb-5b07-4d33-9ad3-a7c212256ee8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2461, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002461__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2462-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2462, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2463, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2784", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2463", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a14a9e08-3450-4361-bccb-bb386ade4e2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2462, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002462__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2463-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2463, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2464, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2785", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2464", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96ebd46c-e85f-480f-973a-d132aec3b2aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 213955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2463, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002463__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2464-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2464, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2465, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2786", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2465", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "453307d2-1e69-4f6b-8fb5-6e6b63cbf13b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2464, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002464__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2465-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2465, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2466, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2787", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2466", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42c23083-bf17-4b6a-830b-c3c2d8c27008", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2465, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002465__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2466-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2466, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "213984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2467, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2788", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2467", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8bd1838f-5818-468b-882f-527266ac0379", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2466, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002466__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2467-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2467, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2468, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2789", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2468", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5dae08aa-dd75-44fb-8d67-761cd5545a13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2467, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002467__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2468-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2468, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2469, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2790", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2469", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9252550-476a-4685-bc37-68ac049db4ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2468, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002468__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2469-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2469, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2470, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2791", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2470", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b98a33e-5d59-42e4-b2e7-09a785a594fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2469, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002469__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2470-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2470, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2471, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2792", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2471", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cdc275b2-4274-4106-b87b-4f4d8883e24d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2470, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002470__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2471-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2471, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2472, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2793", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2472", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90f3276d-9ab9-47f7-8278-6e3128e3a344", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2471, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002471__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2472-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2472, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2473, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2794", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2473", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57083e1b-77b7-4be7-a948-56b4b6e4e3f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2472, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002472__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2473-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2473, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2474, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2795", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2474", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac4652d0-78cd-48d1-af44-a321cc55321d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2473, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002473__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2474-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2474, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2475, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2796", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2475", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f97526c-50e7-4279-a1fb-76f7f2d0ddca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2474, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002474__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2475-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2475, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2476, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2797", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2476", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37821e05-4e07-4b7c-a82b-31fdc27b2d6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 214986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2475, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002475__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2476-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2476, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2477, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2798", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2477", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5681f99e-615c-437b-a7c4-87019efc5fa8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2476, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002476__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2477-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2477, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "214937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2478, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2799", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2478", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c45985ce-c72e-43f8-bd63-032ae218283e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2477, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002477__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2478-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2478, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2479, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2800", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2479", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "517fbc1b-a56d-4413-b466-cc487ac651bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2478, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002478__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2479-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2479, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2480, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2801", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2480", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37fca3fc-6e09-46b8-a814-cc07d76cf9f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2479, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002479__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2480-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2480, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2481, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2802", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2481", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f7ba405-b9c7-461f-a7f0-e64ee3fb5ed3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2480, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002480__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2481-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2481, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2482, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2803", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2482", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f67a6be4-ef28-4ddf-b1e0-73d4d82046cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2481, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002481__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2482-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2482, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2483, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2804", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2483", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05ce6408-217f-44c5-8385-ad58a18078f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2482, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002482__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2483-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2483, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2484, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2805", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2484", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ff32943-69cd-4e4e-bc05-13f96f2ac3fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2483, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002483__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2484-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2484, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2485, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2806", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2485", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d48b6a2-0590-4f7b-bbe6-7a34af5f626d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2484, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002484__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2485-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2485, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2486, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2807", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2486", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "406c0588-66d9-4572-beff-efb72b941810", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2485, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002485__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2486-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2486, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2487, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2808", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2487", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94397da5-a382-414e-98dd-8fc7f7d9eef0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 215940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2486, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002486__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2487-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2487, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2488, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2809", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2488", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf9edeec-ae90-4e40-9582-929176bbab05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2487, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002487__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2488-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2488, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2489, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2810", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2489", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36a4d3fb-fc0b-4156-90f4-4b655a194d4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2488, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002488__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2489-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2489, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "215984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2490, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2811", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2490", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53907137-d03c-4d36-818c-96d15c8b392f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2489, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002489__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2490-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2490, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2491, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2812", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2491", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59980a1b-3ffb-42ea-83b5-2612c7ebd05b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2490, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002490__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2491-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2491, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2492, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2813", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2492", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4690dfd-d86e-4ab0-9742-ffb5ca1f8e92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2491, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002491__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2492-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2492, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2493, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2814", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2493", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65195338-3105-4fbf-b8f6-015153630bd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2492, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002492__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2493-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2493, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2494, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2815", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2494", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b9c4a3a-b54c-44a1-b795-3ba3dce39720", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2493, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002493__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2494-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2494, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2495, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2816", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2495", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "545fbe1c-015c-480d-b580-4018e4ca1e21", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2494, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002494__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2495-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2495, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2496, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2817", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2496", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79a76c61-839d-4bc0-bd87-7f20991bad61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2495, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002495__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2496-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2496, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2497, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2818", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2497", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "652d5a20-d540-4b66-9739-7a489d016a84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2496, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002496__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2497-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2497, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2498, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2819", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2498", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f1865ac-333e-4d2b-9307-7f761eaf16b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2497, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002497__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2498-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2498, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2499, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2820", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2499", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b075821e-e558-4a8e-a510-feb17c7bd9ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 216986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2498, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002498__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2499-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2499, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2500, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2821", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2500", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cc66dc8-0768-48b4-82dd-32b3be64288b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2499, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002499__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2500-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2500, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "216937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2501, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2822", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2501", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3593ff17-04d6-44d0-87bf-1143ade7b7e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2500, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002500__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2501-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2501, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2502, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2823", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2502", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd69daa0-55ed-4996-9ccd-386556cb8c9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2501, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002501__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2502-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2502, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2503, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2824", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2503", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1e96e7e-7c79-4458-ab74-4daf454dcf84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2502, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002502__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2503-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2503, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2504, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2825", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2504", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "188b18f9-76ec-47e6-94fb-23784a6e8c9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2503, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002503__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2504-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2504, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2505, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2826", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2505", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0869f53c-d243-43f0-b1af-7e413a388c59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2504, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002504__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2505-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2505, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2506, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2827", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2506", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a853369-d002-47f9-8c09-039719ff4f47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2505, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002505__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2506-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2506, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2507, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2828", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2507", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74f67688-9521-4176-9de5-1814391f0898", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2506, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002506__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2507-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2507, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2508, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2829", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2508", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "836f2184-56e4-4a5e-89b4-c8b7f0a1d885", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2507, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002507__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2508-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2508, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2509, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2830", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2509", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41c0af80-ed1b-4809-a02d-2f75545c3eea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2508, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002508__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2509-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2509, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2510, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2831", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2510", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b8623be-fd92-4d24-b18f-5af7856b039f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 217940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2509, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002509__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2510-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2510, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2511, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2832", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2511", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "190d9c0f-dd41-4252-8ea9-af168be010c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2510, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002510__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2511-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2511, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2512, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2833", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2512", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5efb6df-99cf-4dfe-bb7f-786d9a379106", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2511, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002511__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2512-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2512, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "217984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2513, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2834", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2513", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "951b3c22-c606-461a-a4dd-2797993eb0f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2512, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002512__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2513-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2513, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2514, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2835", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2514", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ddc26bd-e9aa-4db6-8a82-cfe56f293486", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2513, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002513__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2514-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2514, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2515, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2836", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2515", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f51d405-83fe-49f7-a100-64ccf29fa8b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2514, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002514__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2515-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2515, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2516, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2837", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2516", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c02d7ce-abb3-4db6-bdc6-2e0db6b9f751", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2515, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002515__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2516-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2516, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2517, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2838", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2517", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fcc4efe7-fce1-4aaf-9d24-3412f61ccc28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2516, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002516__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2517-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2517, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2518, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2839", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2518", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60328add-45c3-47e9-a2af-dd11bed3c528", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2517, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002517__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2518-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2518, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2519, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2840", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2519", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97916529-8745-4290-8f1c-6cb8666f94a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2518, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002518__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2519-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2519, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2520, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2841", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2520", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82f365ae-ed2c-4bf7-93d0-232de39dab76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2519, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002519__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2520-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2520, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2521, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2842", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2521", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "660125b6-1f48-4bd7-8610-ade9ede2abf8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2520, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002520__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2521-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2521, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2522, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2843", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2522", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2fb9fa30-61e7-4816-8251-b8c94f28c5f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 218986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2521, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002521__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2522-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2522, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2523, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2844", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2523", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f742c7fe-81e0-4d3c-acaf-9dd14e12a239", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2522, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002522__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2523-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2523, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "218937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2524, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2845", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2524", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8bc64c7-ddbb-420d-b7e2-7a7e3952b94d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2523, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002523__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2524-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2524, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2525, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2846", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2525", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2c4041e-cd63-49c2-8609-57917f296a5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2524, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002524__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2525-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2525, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2526, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2847", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2526", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fb75f05-4e36-4118-a50c-41acd2d6b867", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2525, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002525__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2526-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2526, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2527, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2848", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2527", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c748a644-da46-45d1-8fef-5126da085afe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2526, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002526__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2527-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2527, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2528, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2849", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2528", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4359412a-a2e0-4358-93cc-42974abc6fe8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2527, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002527__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2528-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2528, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2529, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2850", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2529", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9980c952-78e9-42f2-8e9e-7eb6eeebb5c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2528, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002528__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2529-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2529, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2530, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2851", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2530", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "546c410e-7008-4054-b13d-0010990b81af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2529, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002529__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2530-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2530, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2531, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2852", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2531", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d9931ba-62c4-4404-8cde-814e961a336d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2530, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002530__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2531-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2531, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2532, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2853", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2532", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f3c16ca-fee1-449a-90c6-89b42098d6ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2531, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002531__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2532-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2532, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2533, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2854", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2533", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10237acf-20a6-49d0-8092-96668b16aea8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 219940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2532, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002532__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2533-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2533, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2534, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2855", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2534", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3252a8f4-c6e8-43b2-920c-cee955c2b643", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2533, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002533__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2534-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2534, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2535, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2856", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2535", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88ed7632-40ef-4272-aa4d-e532e7acfb9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2534, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002534__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2535-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2535, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "219968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2536, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2857", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2536", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50c209e8-7206-42b3-be96-554eb8ce8e03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2535, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002535__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2536-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2536, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2537, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2858", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2537", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7032cb9e-09d2-4cb4-93d1-94c3e0e159e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2536, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002536__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2537-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2537, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2538, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2859", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2538", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ccee54c-9cec-4b4f-86bf-5d358ad4ce85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2537, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002537__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2538-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2538, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2539, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2860", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2539", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8eb6376c-87c1-4227-980e-da7aae04f070", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2538, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002538__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2539-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2539, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2540, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2861", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2540", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9382f9a-2875-4514-963b-9e94e8f8bfd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2539, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002539__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2540-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2540, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2541, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2862", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2541", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3822319-d65c-434a-a65f-b044448f848b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2540, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002540__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2541-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2541, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2542, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2863", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2542", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09ed9d30-1328-44c5-b784-7fd225be058f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2541, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002541__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2542-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2542, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2543, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2864", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2543", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b89399ca-b54c-4a5e-9455-0edd59f34f04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2542, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002542__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2543-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2543, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2544, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2865", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2544", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6359de7c-80b5-4bea-ad26-0731e8e89238", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2543, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002543__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2544-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2544, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2545, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2866", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2545", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b1f62f2-9cea-4ffa-9d96-2beee3d1c489", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 220971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2544, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002544__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2545-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2545, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2546, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2867", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2546", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c436a5ec-2112-4db7-8155-64215658b862", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2545, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002545__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2546-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2546, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "220921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2547, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2868", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2547", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16b47ca4-9de3-48d0-a2c4-ea38dacc8589", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2546, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002546__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2547-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2547, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2548, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2869", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2548", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe1cb090-7233-420d-9832-3a419a404d49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2547, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002547__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2548-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2548, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2549, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2870", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2549", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6d1beaa-a96c-47a2-9078-5c48f6a4249e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2548, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002548__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2549-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2549, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2550, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2871", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2550", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbd8cec9-b57a-4b1e-92b8-be6640210319", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2549, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002549__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2550-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2550, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2551, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2872", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2551", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb7929c6-8b21-4a2a-abc8-2b8a34dd2ce5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2550, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002550__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2551-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2551, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2552, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2873", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2552", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae883f3d-ec96-4976-aa59-13de4d85c3f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2551, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002551__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2552-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2552, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2553, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2874", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2553", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0ca22cc-f040-4b85-bc8c-56df80cf07dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2552, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002552__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2553-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2553, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2554, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2875", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2554", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95b5f56d-3ec1-49e6-ae5a-8ee8332b74b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2553, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002553__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2554-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2554, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2555, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2876", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2555", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "204ac38f-f8f5-4f23-8713-e45129c32425", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2554, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002554__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2555-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2555, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2556, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2877", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2556", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5b0904d-26af-48dd-84ce-847f41fc0aa4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 221924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2555, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002555__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2556-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2556, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2557, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2878", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2557", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1485c72f-0718-4bff-8a78-2b7e15925488", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2556, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002556__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2557-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2557, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2558, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2879", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2558", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f1e33d8-e254-411e-9db5-ac8a8b2c57ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2557, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002557__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2558-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2558, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "221968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2559, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2880", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2559", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1da09573-e32e-41ec-8fd6-436ab8dba97c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2558, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002558__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2559-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2559, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2560, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2881", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2560", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0594b54f-3fd6-4fbb-a5e9-b0ea4e2195ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2559, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002559__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2560-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2560, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2561, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2882", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2561", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b7c9c7c-3bfa-4ced-8962-4d72a1541330", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2560, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002560__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2561-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2561, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2562, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2883", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2562", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b008789c-0a5a-4c89-b639-b2970d8b16eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2561, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002561__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2562-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2562, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2563, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2884", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2563", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "713f69e6-c888-4674-8347-18a009aebd18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2562, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002562__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2563-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2563, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2564, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2885", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2564", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e556556-964d-467a-8c74-d235b81a3433", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2563, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002563__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2564-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2564, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2565, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2886", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2565", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b721d40-e1cb-4f6a-bdb3-5611178b4016", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2564, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002564__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2565-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2565, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2566, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2887", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2566", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bef04fd-f27b-4271-a161-7a67dc5faf89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2565, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002565__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2566-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2566, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2567, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2888", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2567", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15a46710-68a6-4c45-854c-288d568f9c5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2566, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002566__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2567-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2567, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2568, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2889", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2568", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa192a83-ab16-41de-aeaf-8b4396374a00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 222971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2567, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002567__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2568-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2568, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2569, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2890", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2569", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ba64509-9a20-43a7-8802-b53f87505508", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2568, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002568__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2569-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2569, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "222921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2570, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2891", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2570", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3a15ba4-bfd6-438b-b361-9a6413a92a52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2569, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002569__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2570-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2570, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2571, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2892", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2571", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12118230-9231-4d8f-8908-ad8ab912cfa2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2570, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002570__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2571-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2571, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2572, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2893", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2572", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2187754-34fb-430c-893f-22115965f0cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2571, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002571__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2572-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2572, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2573, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2894", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2573", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "168e70f4-8f8f-4564-b9c5-cd252ecfa96b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2572, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002572__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2573-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2573, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2574, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2895", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2574", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fe60286-2722-4baa-abef-87de41950385", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2573, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002573__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2574-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2574, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2575, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2896", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2575", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46820f93-1ff8-4812-8efc-504cede9b671", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2574, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002574__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2575-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2575, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2576, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2897", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2576", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9cb97def-9dd3-4fd6-ac38-9249ada0fbfa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2575, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002575__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2576-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2576, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2577, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2898", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2577", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81d4a7da-f60d-4b96-bb57-a12a944270e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2576, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002576__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2577-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2577, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2578, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2899", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2578", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90118291-7157-4bdb-af40-9815d3da1bef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2577, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002577__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2578-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2578, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2579, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2900", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2579", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "457ec961-6c95-4a00-9dd0-65ce7436285a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 223924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2578, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002578__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2579-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2579, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2580, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2901", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2580", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecda2b40-2e67-4ca2-b0a7-d8cce5b1d5d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2579, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002579__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2580-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2580, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2581, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2902", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2581", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52607533-c15e-4026-ad91-151c9d8f90d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2580, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002580__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2581-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2581, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "223968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2582, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2903", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2582", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e43aea3-f0a9-40c2-9210-4d4d31d34440", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2581, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002581__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2582-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2582, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2583, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2904", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2583", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "612a8617-8cdf-4b9c-b008-d04bb0cc1fb2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2582, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002582__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2583-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2583, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2584, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2905", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2584", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e6be3d4-3b2c-4663-8d90-8331b5a72aaa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2583, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002583__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2584-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2584, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2585, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2906", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2585", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d3f22ab-7862-4fe0-bd33-93deba8f8849", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2584, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002584__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2585-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2585, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2586, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2907", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2586", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22af38d7-dbf2-4385-ae7f-8f5c1444b1e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2585, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002585__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2586-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2586, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2587, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2908", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2587", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43f7a27c-4dcd-400b-9f4c-ab56ec392ae9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2586, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002586__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2587-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2587, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2588, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2909", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2588", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af14ea71-2695-486d-a79d-e1617b3bb274", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2587, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002587__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2588-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2588, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2589, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2910", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2589", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ab41f68-1416-44c7-afa0-8f4f01dfb84c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2588, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002588__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2589-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2589, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2590, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2911", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2590", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5fc6034-f210-4931-83f4-d614288fcd35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2589, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002589__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2590-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2590, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2591, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2912", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2591", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04587e60-941a-4d63-a51b-64975f8e3ab9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 224971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2590, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002590__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2591-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2591, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2592, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2913", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2592", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b74be770-5314-44fd-b4c4-c36840e2b7ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2591, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002591__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2592-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2592, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "224921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2593, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2914", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2593", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "484d22df-07bb-44de-af08-6f02f9a69395", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2592, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002592__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2593-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2593, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2594, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2915", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2594", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "787d83db-3597-478f-a9f8-b22e19395ca8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2593, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002593__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2594-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2594, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2595, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2916", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2595", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1cda36fa-50cf-45b0-bedc-ad00650b5f26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2594, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002594__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2595-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2595, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2596, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2917", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2596", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d14bcd81-afa2-48ac-97e5-28c35ae90c19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2595, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002595__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2596-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2596, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2597, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2918", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2597", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3f4972f-5776-4801-abfd-0b7336dc5035", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2596, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002596__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2597-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2597, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2598, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2919", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2598", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3f83384-0c39-40c8-b307-1e15352de048", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2597, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002597__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2598-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2598, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2599, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2920", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2599", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d86cbf35-e322-40d6-bc7d-b17c58f999a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2598, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002598__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2599-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2599, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2600, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2921", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2600", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b0ef3d4-8e3a-4864-9a6d-8a5b6ef1276d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2599, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002599__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2600-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2600, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2601, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2922", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2601", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d442425d-700b-4f2d-9ed3-ae13ee773aa2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2600, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002600__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2601-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2601, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2602, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2923", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2602", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "281ed936-3b7c-4d54-9e40-4ec4b604ee9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 225924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2601, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002601__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2602-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2602, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2603, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2924", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2603", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21a87d92-6e9c-4732-8818-41d4fb6ff029", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2602, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002602__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2603-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2603, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2604, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2925", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2604", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16670336-a613-4ba1-803e-b3ad2c629e3c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2603, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002603__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2604-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2604, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "225953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2605, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2926", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2605", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8bf57e3-675f-460e-b133-c6038f4835b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2604, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002604__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2605-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2605, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2606, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2927", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2606", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72af058e-3637-4a44-8664-4b1cfa45b780", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2605, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002605__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2606-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2606, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2607, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2928", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2607", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fbc493f-fd5c-4029-b000-030ad9e3b0e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2606, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002606__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2607-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2607, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2608, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2929", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2608", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f9a69f9-b5bd-4eb2-9b46-c524cce9eb18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2607, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002607__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2608-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2608, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2609, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2930", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2609", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2c607c0-d108-4119-898c-95ddb2ed9602", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2608, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002608__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2609-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2609, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2610, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2931", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2610", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53b5a8f9-ec8e-475e-b67d-b3a1029f05bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2609, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002609__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2610-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2610, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2611, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2932", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2611", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a8aa903a-d945-4353-ab21-aefb1116d753", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2610, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002610__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2611-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2611, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2612, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2933", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2612", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbca7c5a-5c58-4d22-8e43-ac1b7c4bdd62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2611, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002611__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2612-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2612, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2613, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2934", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2613", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70e32344-4fcf-4777-95ea-6cbabb013c1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2612, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002612__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2613-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2613, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2614, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2935", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2614", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b032415e-31b4-4865-bdba-c6a697607472", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 226955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2613, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002613__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2614-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2614, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2615, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2936", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2615", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "968dcf69-baed-4838-903f-a8de369ee497", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2614, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002614__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2615-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2615, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "226906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2616, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2937", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2616", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2d41443-35d0-44e1-afcb-b3c43c4781aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2615, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002615__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2616-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2616, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2617, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2938", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2617", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aec3adfd-c20d-4fb5-86de-578e39769884", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2616, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002616__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2617-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2617, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2618, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2939", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2618", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf62f999-7b49-40ea-be10-933953935b8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2617, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002617__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2618-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2618, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2619, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2940", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2619", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67a9035c-1e76-4186-878d-13f537d86d28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2618, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002618__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2619-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2619, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2620, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2941", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2620", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d963cfea-f613-43b0-8ce7-b275d5da86f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2619, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002619__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2620-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2620, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2621, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2942", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2621", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9e5c146-43fc-46e3-ab96-4620f671dc69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2620, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002620__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2621-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2621, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2622, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2943", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2622", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26f27400-d63a-487f-a6cb-68edb1adc441", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2621, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002621__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2622-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2622, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2623, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2944", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2623", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19f46233-a062-4016-8b8f-81a0a5dc7cd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2622, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002622__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2623-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2623, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2624, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2945", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2624", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39079cc3-1a79-4025-ac5f-aebcb4334a77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2623, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002623__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2624-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2624, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2625, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2946", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2625", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2216191-867c-4a35-b869-70c13a783145", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 227908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2624, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002624__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2625-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2625, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2626, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2947", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2626", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "155f4c33-6e95-4701-9e8e-ecab06391dae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2625, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002625__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2626-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2626, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2627, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2948", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2627", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a27dd70a-4d83-461c-bd56-b34b574e8ece", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2626, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002626__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2627-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2627, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "227953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2628, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2949", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2628", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13716dcd-4bdc-4716-982f-a66c9bfce1b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2627, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002627__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2628-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2628, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2629, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2950", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2629", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1de50886-fa5a-4a89-9afc-44828eed4bda", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2628, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002628__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2629-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2629, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2630, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2951", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2630", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e49d1c3-31a4-4aa8-a09a-7ff33296b546", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2629, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002629__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2630-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2630, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2631, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2952", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2631", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cafd7327-4e10-4ed3-9042-ac9a682bbf62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2630, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002630__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2631-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2631, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2632, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2953", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2632", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2ba76d6-b9ac-45ca-ab96-ec6e3036ce60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2631, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002631__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2632-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2632, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2633, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2954", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2633", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "788377ce-c603-46a2-8063-2e8c5a2430f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2632, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002632__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2633-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2633, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2634, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2955", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2634", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a46b7a8-124f-4080-9eab-398bc6b16209", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2633, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002633__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2634-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2634, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2635, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2956", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2635", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d405081-b2d1-44e4-9a76-775458121b7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2634, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002634__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2635-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2635, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2636, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2957", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2636", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8e3ef53-dd8b-44f8-99d7-77fe222fb056", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2635, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002635__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2636-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2636, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2637, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2958", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2637", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f50b9746-6052-4764-8015-7af7f851a12d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 228955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2636, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002636__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2637-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2637, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2638, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2959", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2638", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bccfcbc-4cc0-4b38-a562-948f96f43d82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2637, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002637__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2638-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2638, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "228906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2639, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2960", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2639", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84cfadf9-3f8a-476f-824a-127f69740282", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2638, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002638__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2639-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2639, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2640, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2961", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2640", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05351cfe-3224-4da2-a6a1-a88c756ce33c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2639, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002639__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2640-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2640, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2641, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2962", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2641", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52141327-9261-4192-98e6-519237374a80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2640, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002640__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2641-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2641, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2642, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2963", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2642", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9cd4be49-2fd8-493b-830e-d32421cb0d45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2641, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002641__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2642-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2642, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2643, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2964", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2643", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3f26f33-fa75-4488-8fc4-5bf2c5d59db1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2642, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002642__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2643-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2643, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2644, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2965", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2644", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1599e755-2f3b-450f-876f-bba50420c286", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2643, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002643__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2644-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2644, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2645, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2966", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2645", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e1c1148-3580-40b6-8058-f92ac5e289af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2644, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002644__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2645-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2645, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2646, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2967", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2646", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9eeacea9-b083-4f22-88b0-e1a79b25d9ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2645, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002645__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2646-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2646, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2647, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2968", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2647", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6a57f68-7f2c-4056-974f-b9754a068b25", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2646, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002646__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2647-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2647, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2648, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2969", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2648", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2119a027-e97d-4c96-b009-6f7c6c6607de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 229908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2647, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002647__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2648-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2648, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2649, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2970", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2649", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6178d5e-aa26-4271-9de0-ee3547778aef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2648, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002648__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2649-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2649, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2650, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2971", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2650", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72efd209-e506-404f-9d8d-2f9eb6d9a0fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2649, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002649__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2650-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2650, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "229953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2651, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2972", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2651", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a3c4640-3ed6-4efb-9fef-f86ae1563278", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2650, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002650__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2651-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2651, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2652, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2973", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2652", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0e38dcf-dc14-4f82-a73e-302681fdf6a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2651, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002651__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2652-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2652, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2653, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2974", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2653", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95b070db-e5a7-4720-b750-2db35dfc61e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2652, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002652__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2653-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2653, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2654, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2975", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2654", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2f3b799-f89f-48fa-a380-d22f333d9e3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2653, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002653__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2654-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2654, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2655, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2976", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2655", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9331a59-c171-4d04-9c1b-d53f2a308487", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2654, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002654__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2655-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2655, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2656, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2977", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2656", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de3ce8fe-1845-47f1-9dee-be16815bba5f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2655, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002655__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2656-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2656, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2657, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2978", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2657", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26238cae-8dba-44bb-8ef4-896898e06e29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2656, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002656__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2657-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2657, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2658, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2979", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2658", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ba27e15-07df-4e1f-bfcc-d3ca9c454156", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2657, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002657__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2658-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2658, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2659, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2980", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2659", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5c12ef1-adad-40f1-a2b8-69b8b2ef4ab9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2658, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002658__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2659-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2659, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2660, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2981", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2660", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "317fd411-adb5-4500-9f06-f3e3f4e36cff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 230955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2659, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002659__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2660-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2660, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2661, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2982", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2661", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d7434c1-d397-4a80-91a9-63abb713ad49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2660, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002660__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2661-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2661, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2662, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2983", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2662", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1866d261-d094-4a8f-a919-ef7c2a6fe564", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2661, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002661__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2662-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2662, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "230984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2663, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2984", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2663", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea7cc490-6e13-4917-81ed-d0cef51865d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2662, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002662__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2663-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2663, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2664, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2985", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2664", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39fbe43e-c73f-4ec1-ae90-633fea2ba18a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2663, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002663__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2664-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2664, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2665, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2986", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2665", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06560000-ad44-4b70-9a57-499348ba2a93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2664, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002664__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2665-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2665, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2666, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2987", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2666", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c66deaa-eb7e-43db-bf2d-4dce73cbea82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2665, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002665__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2666-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2666, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2667, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2988", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2667", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "680a84fb-8458-4a87-b4aa-c468a9de77fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2666, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002666__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2667-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2667, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2668, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2989", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2668", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b548a7f3-a486-4437-bb37-4b904f94fa90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2667, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002667__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2668-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2668, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2669, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2990", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2669", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36397ed0-0e14-461b-9f9c-74bea28de49e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2668, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002668__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2669-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2669, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2670, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2991", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2670", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95596968-fd8a-481f-993d-608f3085aca1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2669, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002669__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2670-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2670, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2671, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2992", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2671", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fb59928-db69-462c-8fdd-59ed5434ff95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2670, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002670__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2671-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2671, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2672, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2993", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2672", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "778ccfaf-70e4-484b-9a3b-420aeda927f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 231986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2671, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002671__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2672-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2672, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2673, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2994", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2673", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "525ec88f-1d60-4ba0-b792-4741d75983b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2672, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002672__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2673-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2673, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "231937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2674, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2995", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2674", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10615a5b-4a37-4f4c-a493-a1f1dae670fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2673, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002673__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2674-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2674, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2675, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2996", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2675", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a473da57-9c27-4181-b081-2302bb484e04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2674, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002674__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2675-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2675, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2676, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2997", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2676", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77289ba6-8f06-4cff-a3af-03ffdc9f72b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2675, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002675__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2676-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2676, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2677, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2998", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2677", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4785f244-22a3-4403-98e9-7f949a539f4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2676, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002676__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2677-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2677, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2678, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "2999", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2678", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9140ff8-1fa2-491f-99c7-74b2a5995531", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2677, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002677__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2678-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2678, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2679, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3000", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2679", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aad5bf2c-e1cc-4010-8d43-71ba6f4878dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2678, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002678__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2679-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2679, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2680, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3001", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2680", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c85622d4-8175-4495-bcb0-104007bf292b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2679, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002679__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2680-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2680, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2681, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3002", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2681", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d893c39e-da32-442c-a2cc-bf4fa7b0f3f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2680, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002680__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2681-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2681, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2682, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3003", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2682", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8079a0a3-5883-4496-a4ce-c1cbebaccc4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2681, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002681__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2682-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2682, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2683, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3004", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2683", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56ffd8fb-a8e4-4860-a988-4ba40e8acb19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 232940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2682, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002682__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2683-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2683, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2684, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3005", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2684", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44f47eea-6104-4364-8a48-eeef23e69bc3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2683, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002683__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2684-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2684, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2685, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3006", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2685", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcc6960d-57d8-4d83-b34e-98718c8fc863", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2684, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002684__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2685-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2685, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "232984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2686, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3007", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2686", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a3a01f9-309b-4463-b01b-3c6874ddda4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2685, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002685__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2686-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2686, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2687, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3008", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2687", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8df6a0ab-23df-4fd0-8ac1-f0c0d25c2b84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2686, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002686__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2687-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2687, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2688, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3009", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2688", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e233aca-a946-49b6-ab96-67df8a5c0bd0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2687, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002687__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2688-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2688, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2689, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3010", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2689", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7119f163-f118-4958-81e3-d33955108836", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2688, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002688__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2689-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2689, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2690, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3011", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2690", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ba3d2f0-f14d-4e41-abd0-2065aa8c3877", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2689, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002689__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2690-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2690, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2691, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3012", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2691", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95df41d0-7970-4bd6-a22c-8060078f51c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2690, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002690__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2691-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2691, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2692, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3013", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2692", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46ab02d5-7db6-418d-a1b8-7097bac722cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2691, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002691__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2692-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2692, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2693, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3014", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2693", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "046d7b94-1ff6-443e-9799-5a12eeb0558f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2692, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002692__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2693-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2693, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2694, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3015", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2694", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df081e8b-5fc9-429d-8e35-d484b1dd902f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2693, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002693__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2694-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2694, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2695, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3016", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2695", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bf574b7-106b-492e-8d30-8d2cf32f3dd0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 233986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2694, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002694__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2695-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2695, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2696, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3017", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2696", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "483b8f8d-c489-41f5-ac68-7ebc861dc9d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2695, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002695__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2696-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2696, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "233937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2697, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3018", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2697", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "affded7f-e50a-44fa-aff1-26e19de9bf19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2696, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002696__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2697-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2697, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2698, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3019", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2698", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b96473b-56c9-48c5-b51a-fab9af4590de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2697, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002697__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2698-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2698, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2699, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3020", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2699", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b3243e9-4ce6-487e-bbef-88701790d23a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2698, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002698__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2699-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2699, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2700, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3021", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2700", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13446bbe-ebbc-420b-bef4-5f4e0510af08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2699, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002699__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2700-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2700, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2701, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3022", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2701", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9af14dcb-9f29-4151-a4ff-47990edfe2e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2700, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002700__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2701-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2701, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2702, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3023", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2702", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8d5d5be-82ab-464c-8e64-10160e63efc2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2701, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002701__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2702-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2702, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2703, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3024", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2703", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47595bba-b7ba-4916-985e-732d30791c6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2702, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002702__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2703-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2703, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2704, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3025", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2704", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86bdc3cf-f0f6-4a9f-9ad2-53ca9d68bb9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2703, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002703__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2704-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2704, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2705, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3026", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2705", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f197edb5-8a5d-488a-b101-d6706134aa12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2704, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002704__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2705-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2705, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2706, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3027", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2706", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06cdac15-e068-40f9-ae1c-68b246e124fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 234940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2705, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002705__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2706-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2706, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2707, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3028", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2707", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03b85917-9916-4d87-b16b-9b82acdcd91e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2706, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002706__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2707-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2707, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2708, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3029", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2708", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06a42ac8-b99d-4b66-a19d-ecccaaa566b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2707, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002707__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2708-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2708, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "234984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2709, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3030", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2709", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bcd95d5-1a0b-4417-b6cb-eeb22b998012", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2708, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002708__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2709-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2709, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2710, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3031", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2710", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f2b7f25-810d-4891-9ad5-0c30fd8acef8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2709, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002709__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2710-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2710, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2711, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3032", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2711", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ada3b3f-f916-4fbc-9692-2ccd617619a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2710, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002710__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2711-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2711, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2712, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3033", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2712", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "836fa347-df99-468e-bd1b-a0e804fe9c19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2711, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002711__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2712-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2712, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2713, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3034", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2713", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86c4e3fc-8edf-4003-84d4-6b8421d62c74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2712, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002712__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2713-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2713, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2714, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3035", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2714", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04ea80ca-6ccd-422b-8306-07101e123323", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2713, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002713__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2714-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2714, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2715, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3036", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2715", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91c8e286-2163-42e5-b6cd-6ab07515127d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2714, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002714__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2715-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2715, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2716, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3037", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2716", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d8d067c-07e4-43d7-8523-3dc9ded1a1a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2715, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002715__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2716-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2716, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2717, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3038", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2717", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "940677e7-20bd-4682-b5eb-a7756de91185", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2716, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002716__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2717-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2717, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2718, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3039", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2718", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02a618a7-1b37-4d9f-83d1-9b4b375e160f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 235986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2717, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002717__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2718-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2718, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2719, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3040", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2719", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "971ed8db-bf97-4b46-8d1f-a5e8986bb3e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2718, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002718__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2719-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2719, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "235937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2720, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3041", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2720", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c725c44-c77c-4e2c-b01b-197a82d7c46d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2719, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002719__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2720-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2720, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2721, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3042", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2721", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c7190e7-12ab-4df1-b08d-d6d07448fa2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2720, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002720__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2721-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2721, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2722, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3043", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2722", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aab443ca-9520-4d76-b7fc-b2ad6f5fcbac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2721, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002721__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2722-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2722, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2723, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3044", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2723", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06a8daa8-465e-41a1-8ccc-2b8039a4c1ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2722, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002722__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2723-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2723, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2724, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3045", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2724", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d78e30b-3a18-4e4f-b794-56455cc052fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2723, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002723__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2724-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2724, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2725, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3046", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2725", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c2e025f-4f58-4e8f-929a-ad62018478f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2724, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002724__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2725-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2725, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2726, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3047", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2726", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b57b7eaf-5d83-4f55-97e3-d30c80a85488", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2725, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002725__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2726-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2726, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2727, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3048", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2727", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ed89196-c6fb-46b0-9b48-6db792813e0e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2726, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002726__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2727-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2727, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2728, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3049", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2728", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5dc1341-6462-41c7-8036-82f24cd6612b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2727, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002727__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2728-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2728, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2729, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3050", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2729", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57155c5e-e84d-4f50-aec3-3ba14e5a30fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 236940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2728, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002728__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2729-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2729, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2730, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3051", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2730", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52a3be17-2176-43e1-b419-e0ca131ca7cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2729, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002729__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2730-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2730, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2731, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3052", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2731", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db7a9f97-db88-4f22-9bd1-340354d0d47d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2730, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002730__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2731-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2731, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "236968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2732, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3053", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2732", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84f4583e-811f-459b-a851-c90d5570587d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2731, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002731__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2732-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2732, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2733, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3054", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2733", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "509a1eff-234b-4d17-8e21-6df6b8dc9a64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2732, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002732__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2733-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2733, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2734, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3055", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2734", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7752f4bf-bab8-4685-8494-70790008cd61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2733, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002733__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2734-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2734, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2735, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3056", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2735", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa98cf0d-9588-4616-8db5-6b8e782bfea7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2734, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002734__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2735-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2735, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2736, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3057", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2736", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85122b82-714e-4f5f-9f3d-390fabbd97df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2735, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002735__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2736-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2736, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2737, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3058", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2737", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fbc79016-f397-4541-8d7d-47ba9719907e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2736, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002736__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2737-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2737, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2738, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3059", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2738", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd6a5d5c-99e4-4611-adf5-ca0830cbd054", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2737, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002737__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2738-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2738, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2739, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3060", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2739", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "270c407a-fe73-49f7-9820-e32ab34730a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2738, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002738__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2739-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2739, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2740, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3061", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2740", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66a2638e-ed97-4525-800d-3feb35a281e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2739, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002739__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2740-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2740, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2741, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3062", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2741", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b0850ef-939b-46b4-964b-d4413b8f07f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 237971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2740, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002740__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2741-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2741, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2742, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3063", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2742", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbd94de7-ed8b-4ff8-9dd6-e6b69928cf02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2741, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002741__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2742-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2742, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "237921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2743, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3064", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2743", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55cb1277-2ca1-47c7-a430-605802643eac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2742, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002742__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2743-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2743, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2744, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3065", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2744", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10d686b4-62e6-4d27-b381-a4d32c09c2fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2743, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002743__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2744-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2744, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2745, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3066", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2745", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca7349c8-bc30-4e54-a8cd-f3fe565669a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2744, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002744__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2745-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2745, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2746, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3067", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2746", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a03c633b-e32e-4283-bb0f-5b0445e1962c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2745, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002745__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2746-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2746, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2747, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3068", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2747", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b68e066a-a964-4814-b276-5d0052746bb2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2746, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002746__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2747-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2747, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2748, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3069", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2748", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95820607-a012-4495-8518-c0e4063b1dc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2747, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002747__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2748-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2748, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2749, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3070", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2749", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24cdd433-8263-40a9-bf28-4c53332038f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2748, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002748__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2749-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2749, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2750, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3071", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2750", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2fd26750-628c-415a-9fa9-982300b557f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2749, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002749__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2750-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2750, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2751, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3072", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2751", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd9a5b8c-58be-4136-99aa-8cdec9d7cb7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2750, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002750__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2751-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2751, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2752, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3073", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2752", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da6c571d-d849-4ca1-bfa6-46fbd7c7e1f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 238924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2751, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002751__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2752-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2752, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2753, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3074", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2753", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0bc0bfc8-de9e-47de-a398-ff3e116f9c6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2752, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002752__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2753-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2753, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2754, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3075", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2754", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32b2face-15bb-4958-8621-5615553979d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2753, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002753__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2754-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2754, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "238968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2755, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3076", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2755", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13070363-36e5-4fea-8ebf-21b074ea62fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2754, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002754__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2755-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2755, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2756, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3077", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2756", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31bff1de-4c8f-4e85-aa5e-421a54ef0072", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2755, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002755__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2756-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2756, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2757, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3078", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2757", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "864b84da-9cc8-4c34-b7ab-ff9110855e16", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2756, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002756__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2757-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2757, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2758, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3079", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2758", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67321d6b-0efc-46c4-89ed-f51b47f87414", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2757, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002757__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2758-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2758, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2759, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3080", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2759", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "533c1534-b2cc-42fe-9cf2-27f462ca6cf4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2758, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002758__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2759-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2759, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2760, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3081", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2760", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0370eda9-58da-4b38-a3e3-26025a1cc91e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2759, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002759__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2760-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2760, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2761, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3082", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2761", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1038dbd-d119-4178-9db5-549df2cc015f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2760, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002760__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2761-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2761, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2762, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3083", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2762", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba7da268-e2be-481a-b254-2e90cd5c6dff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2761, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002761__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2762-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2762, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2763, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3084", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2763", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a3bd49e-7aad-401a-ae39-c3833672b705", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2762, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002762__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2763-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2763, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2764, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3085", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2764", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f1b0c80-1e8f-42ea-af3d-08bce01203eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 239971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2763, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002763__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2764-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2764, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2765, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3086", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2765", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4270599b-10b1-43cc-afb0-2033099d7590", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2764, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002764__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2765-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2765, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "239921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2766, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3087", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2766", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2a88336-8589-41bc-a482-8e395d7428e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2765, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002765__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2766-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2766, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2767, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3088", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2767", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da9784df-cbfa-42b6-9284-0931d36ffd60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2766, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002766__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2767-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2767, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2768, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3089", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2768", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "741881f7-483b-44f3-852f-7365efc2793b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2767, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002767__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2768-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2768, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2769, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3090", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2769", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd6af77d-dd00-425b-aced-de957b5dced4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2768, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002768__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2769-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2769, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2770, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3091", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2770", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42a3a924-0136-4189-a77e-b59e81ce0706", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2769, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002769__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2770-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2770, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2771, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3092", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2771", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af492e7d-2ae9-4273-a397-1d91187c3872", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2770, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002770__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2771-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2771, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2772, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3093", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2772", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "35be338b-8d3d-4bea-a47d-859768a5a28f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2771, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002771__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2772-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2772, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2773, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3094", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2773", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab67334a-9743-4e4d-b005-b49de5b80e72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2772, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002772__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2773-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2773, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2774, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3095", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2774", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c7d40e4-355e-4dec-b841-12ce752f4092", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2773, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002773__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2774-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2774, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2775, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3096", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2775", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91f168e4-8add-45ee-9caf-76fb036e9016", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 240924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2774, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002774__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2775-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2775, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2776, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3097", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2776", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88676800-b184-446b-89dd-fc50df0f2603", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2775, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002775__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2776-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2776, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2777, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3098", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2777", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c86eaf9-88c0-4ac5-8ce6-6632c74be9d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2776, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002776__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2777-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2777, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "240968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2778, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3099", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2778", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c8dcc68-af54-4c9e-9652-1e972c7ec18c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2777, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002777__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2778-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2778, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2779, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3100", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2779", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50abbd71-744a-4923-bb14-d852dd4865fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2778, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002778__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2779-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2779, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2780, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3101", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2780", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3060901c-b82d-4d2f-b0b1-57e5b6b12799", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2779, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002779__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2780-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2780, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2781, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3102", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2781", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08aa1eb3-de12-4457-a1e8-8fd50663dd3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2780, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002780__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2781-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2781, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2782, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3103", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2782", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "710973f8-ee65-443a-9a05-d4e2242ee771", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2781, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002781__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2782-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2782, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2783, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3104", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2783", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c78595c-f9ce-4ca6-941c-d2ab01c3a7da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2782, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002782__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2783-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2783, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2784, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3105", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2784", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1b21977-863a-46d4-81c2-c27c74d804d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2783, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002783__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2784-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2784, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2785, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3106", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2785", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "623a6733-9222-4d5c-99c3-06feb573a669", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2784, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002784__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2785-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2785, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2786, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3107", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2786", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fcc9ab0-afea-46ba-861f-3407e1d45e8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2785, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002785__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2786-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2786, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2787, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3108", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2787", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0bbda756-d29a-4b50-91d3-8f5f0cda4da2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 241971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2786, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002786__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2787-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2787, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2788, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3109", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2788", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef0328ae-a84a-4c26-a561-26a19d9a9fb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2787, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002787__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2788-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2788, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "241921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2789, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3110", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2789", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46e636fd-78a7-42c9-b944-553e388e281e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2788, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002788__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2789-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2789, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2790, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3111", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2790", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ddc8dfc-5c26-401e-a106-431e2733dec0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2789, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002789__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2790-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2790, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2791, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3112", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2791", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0e95d6b-cb64-4dff-b45f-0e2c43118a2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2790, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002790__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2791-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2791, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2792, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3113", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2792", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ca43cdd-6945-48c5-bb91-ebbe33a882a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2791, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002791__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2792-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2792, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2793, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3114", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2793", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8906be16-e0d9-46d5-8379-29216a61f427", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2792, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002792__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2793-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2793, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2794, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3115", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2794", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65078fd3-b24d-4742-82b8-020827b083e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2793, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002793__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2794-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2794, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2795, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3116", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2795", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c527249d-c458-4c34-99e9-5bfd3c031c45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2794, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002794__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2795-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2795, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2796, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3117", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2796", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26cc707c-ba15-43c9-bc70-1393895e6123", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2795, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002795__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2796-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2796, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2797, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3118", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2797", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05c7a7c7-63d8-40f9-95a9-bafdbd6eed12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2796, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002796__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2797-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2797, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2798, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3119", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2798", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c350471-32ed-4f88-9d8f-bdd76451a64a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 242924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2797, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002797__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2798-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2798, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2799, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3120", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2799", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e92b3155-e1ef-4b43-bf1f-69cd24b44789", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2798, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002798__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2799-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2799, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2800, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3121", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2800", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b65c789-6e2b-48e6-851c-d59d14d560bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2799, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002799__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2800-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2800, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "242953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2801, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3122", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2801", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef8a9e22-8f44-4753-b3d8-1cafcb85a178", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2800, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002800__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2801-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2801, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2802, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3123", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2802", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ddb22c9-2bc4-439f-9883-b61164e861e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2801, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002801__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2802-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2802, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2803, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3124", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2803", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01c9fdbb-3246-497c-a79a-521cadc485b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2802, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002802__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2803-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2803, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2804, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3125", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2804", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9840643-5f1c-4a62-b7ce-53b02cffe4ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2803, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002803__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2804-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2804, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2805, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3126", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2805", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "508c294d-e836-4991-b047-cd4438df7df2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2804, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002804__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2805-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2805, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2806, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3127", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2806", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88a744bc-211b-434d-95ed-a79c8ffd30c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2805, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002805__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2806-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2806, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2807, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3128", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2807", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d49385a9-5f3f-4caa-8e02-57ed1e0f8b92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2806, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002806__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2807-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2807, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2808, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3129", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2808", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f44b77c-83d6-4e76-ad64-8464d9e2c43c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2807, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002807__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2808-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2808, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2809, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3130", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2809", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7ab84ba-b9a6-43b1-ab77-64eca12cdb43", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2808, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002808__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2809-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2809, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2810, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3131", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2810", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28003c99-7fea-4861-8c85-d669cd34d0ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2809, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002809__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2810-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2810, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2811, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3132", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2811", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0e3543b-8d82-45f1-bdaf-6f74139e195c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2810, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002810__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2811-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2811, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "243906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2812, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3133", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2812", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f00e87e1-c1c9-4479-a837-09a63b75c167", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2811, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002811__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2812-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2812, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2813, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3134", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2813", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de50fba3-bf72-4874-8f0c-f426ea1a44cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2812, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002812__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2813-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2813, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2814, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3135", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2814", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f731a8e-c07c-43f1-8d17-84ebcc83b66d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2813, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002813__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2814-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2814, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2815, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3136", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2815", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69a43550-d2b8-4512-b6c7-2bbe2c56e54f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2814, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002814__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2815-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2815, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2816, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3137", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2816", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e7b0a9c-9e9d-44f9-8bce-639e7c5c7a28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2815, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002815__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2816-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2816, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2817, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3138", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2817", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f35b0a0e-cd53-4931-80c7-62b6c20efc8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2816, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002816__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2817-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2817, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2818, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3139", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2818", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9e2f7b1-1348-424d-952d-78598333c6d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2817, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002817__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2818-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2818, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2819, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3140", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2819", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74fbee00-a3ff-4a77-91d6-93e60d997d40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2818, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002818__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2819-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2819, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2820, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3141", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2820", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6b5fc5c-5a96-4d0a-8e96-c11b04330882", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2819, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002819__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2820-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2820, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2821, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3142", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2821", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0df44f54-9263-4452-aab5-313988ccc251", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 244908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2820, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002820__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2821-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2821, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2822, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3143", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2822", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae56eafc-3537-46ae-97f6-f914c2120bd4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2821, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002821__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2822-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2822, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2823, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3144", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2823", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31ea1ac7-e4b5-4244-b24a-c6b22056d0af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2822, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002822__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2823-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2823, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "244953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2824, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3145", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2824", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "459412c8-d677-490c-a58a-9d22f5a4948a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2823, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002823__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2824-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2824, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2825, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3146", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2825", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "edbae815-a47a-4bde-9a41-7982f093edd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2824, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002824__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2825-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2825, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2826, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3147", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2826", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf8241ba-0240-477a-a803-c06937eeef49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2825, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002825__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2826-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2826, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2827, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3148", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2827", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fcaf942-9434-4512-ac29-634b574aacb8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2826, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002826__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2827-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2827, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2828, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3149", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2828", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56f06105-eba3-46d7-b6fe-b078b7cb7e6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2827, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002827__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2828-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2828, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2829, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3150", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2829", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3dccf30-11ab-4314-ac5f-b99cf6b839d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2828, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002828__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2829-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2829, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2830, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3151", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2830", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "646e2a46-ea5d-40d7-b956-cee99651acd2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2829, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002829__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2830-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2830, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2831, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3152", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2831", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05bae854-4e0c-4e55-b1fb-d5266ad353df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2830, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002830__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2831-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2831, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2832, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3153", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2832", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f12337e-bbbc-4882-92e8-09e42a1cb97f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2831, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002831__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2832-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2832, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2833, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3154", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2833", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "419f8e2f-1be7-41be-9892-b3145af62c36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 245955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2832, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002832__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2833-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2833, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2834, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3155", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2834", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50aa41a0-7a18-4193-b6e9-c4632baa19e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2833, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002833__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2834-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2834, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "245906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2835, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3156", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2835", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "420e1809-5be1-4b2b-a082-c361eb0704b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2834, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002834__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2835-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2835, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2836, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3157", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2836", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f3ae2eb-32c2-456d-91c6-c8d1b98e031d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2835, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002835__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2836-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2836, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2837, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3158", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2837", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96a88394-d5a0-47c7-9091-620fa4e86f5a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2836, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002836__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2837-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2837, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2838, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3159", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2838", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37eb9748-dd53-484b-817d-868df1b5aef0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2837, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002837__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2838-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2838, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2839, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3160", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2839", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "918a35aa-034f-4f89-b543-a0f61ae06027", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2838, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002838__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2839-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2839, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2840, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3161", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2840", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5318f6f-5776-4a59-8acc-f3f94d5ea4a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2839, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002839__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2840-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2840, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2841, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3162", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2841", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f2aad1e-e080-4562-9acf-7bb39b733f6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2840, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002840__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2841-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2841, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2842, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3163", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2842", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6a278c4-cf7c-4726-92ad-3716d2a19e02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2841, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002841__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2842-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2842, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2843, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3164", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2843", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "070e2270-620f-410c-bd79-6f3f32fb2c95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2842, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002842__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2843-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2843, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2844, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3165", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2844", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c5e084c-a903-4036-a057-ad988a862c3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 246908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2843, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002843__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2844-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2844, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2845, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3166", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2845", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dac7e0bb-5285-47ee-8f47-7e38b9394ec6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2844, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002844__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2845-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2845, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2846, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3167", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2846", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "deb8cb39-9a91-4e06-a4db-d554e0d68e38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2845, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002845__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2846-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2846, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "246953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2847, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3168", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2847", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dfd7074c-3765-4d13-abd6-c41dc7707774", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2846, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002846__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2847-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2847, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2848, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3169", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2848", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "213c0971-4c9d-4e75-bdc9-5c7748673de7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2847, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002847__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2848-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2848, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2849, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3170", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2849", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ec5e1d6-1383-4728-b842-84b3c620b5f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2848, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002848__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2849-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2849, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2850, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3171", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2850", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cade11ef-ed93-4871-88c2-8adae9e0241e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2849, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002849__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2850-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2850, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2851, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3172", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2851", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "220124e9-ae92-4c90-8a6f-34ceafcae19d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2850, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002850__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2851-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2851, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2852, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3173", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2852", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da14bd21-9575-4fb1-9e74-f750c1434785", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2851, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002851__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2852-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2852, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2853, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3174", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2853", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3d50d28-01f3-4e7e-bb46-dadd497d93ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2852, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002852__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2853-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2853, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2854, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3175", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2854", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2d62dd4-4e1e-44a6-9010-eece902efc8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2853, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002853__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2854-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2854, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2855, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3176", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2855", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60b2331f-4e67-468c-a9b7-1e2f03d5d7b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2854, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002854__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2855-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2855, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2856, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3177", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2856", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c24180d1-f377-4eaf-8769-92ecdbdc2ccd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 247955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2855, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002855__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2856-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2856, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2857, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3178", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2857", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29383b12-ba00-4429-9bf3-e9538821dfee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2856, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002856__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2857-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2857, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2858, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3179", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2858", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ad621d2-cb69-46ca-bfc1-05507ddce207", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2857, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002857__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2858-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2858, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "247984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2859, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3180", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2859", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe4015e3-aa63-445e-851e-134ec717a843", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2858, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002858__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2859-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2859, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2860, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3181", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2860", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c1a12b2-09c0-422f-aa86-7dfc39ab8eba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2859, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002859__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2860-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2860, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2861, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3182", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2861", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5799ddf-52f6-4112-95ec-a750204f49ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2860, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002860__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2861-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2861, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2862, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3183", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2862", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d7415fd-7919-4259-9275-e8335522eae9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2861, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002861__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2862-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2862, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2863, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3184", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2863", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30db22b9-5fe2-4f9a-a5ec-1c63cd2d2ab3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2862, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002862__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2863-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2863, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2864, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3185", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2864", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23dbc6ec-7842-4fad-9bfb-f77d28811c7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2863, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002863__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2864-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2864, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2865, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3186", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2865", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "470d81ca-91f4-455f-9615-a0262b7467d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2864, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002864__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2865-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2865, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2866, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3187", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2866", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34d60849-ea1c-44b3-bfe4-c10fcc68073b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2865, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002865__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2866-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2866, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2867, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3188", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2867", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85323d7b-fa3b-43ad-bcc1-e1ff8bf13ffb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2866, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002866__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2867-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2867, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2868, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3189", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2868", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee53ac2e-d00f-4fec-82b8-8fc92deb12f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 248986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2867, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002867__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2868-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2868, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2869, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3190", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2869", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91aafbbe-2749-4aab-9a0f-e5f41822a698", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2868, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002868__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2869-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2869, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "248937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2870, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3191", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2870", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f01e1df-efa6-4279-b7f6-284e0d5a3f6f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2869, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002869__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2870-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2870, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2871, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3192", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2871", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac399b40-716a-49b7-b0ca-54e2057235ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2870, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002870__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2871-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2871, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2872, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3193", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2872", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56eea551-ad13-4807-9b70-2f27d23d1dea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2871, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002871__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2872-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2872, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2873, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3194", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2873", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8d30b95-118c-40c9-bc28-9e23b68cd9e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2872, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002872__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2873-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2873, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2874, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3195", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2874", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f9be36e-608a-4b36-b329-5b81e96acb3d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2873, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002873__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2874-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2874, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2875, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3196", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2875", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37521e00-d034-4687-96b7-a03d5d25c2d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2874, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002874__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2875-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2875, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2876, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3197", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2876", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc4212a5-794c-4eeb-878e-5bcde81d0406", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2875, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002875__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2876-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2876, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2877, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3198", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2877", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b502b463-68a6-4d66-bab0-786d5c1f47aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2876, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002876__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2877-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2877, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2878, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3199", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2878", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ea2fa54-e414-48b5-bb4e-0fb7c8d11da0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2877, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002877__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2878-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2878, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2879, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3200", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2879", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51bcf922-500d-4f76-8a68-fb0125ca1ca9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 249940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2878, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002878__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2879-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2879, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2880, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3201", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2880", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31b3bbe6-828a-41af-9bff-de15767e4fa8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2879, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002879__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2880-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2880, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2881, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3202", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2881", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00ded9b7-4022-4f6c-ac15-6d8935338816", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2880, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002880__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2881-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2881, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "249984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2882, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3203", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2882", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "813435ec-4990-43cc-9bc6-393e4ef907f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2881, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002881__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2882-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2882, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2883, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3204", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2883", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c97c7886-7c92-4f10-abcf-a5782bd46df9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2882, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002882__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2883-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2883, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2884, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3205", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2884", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a230e53-4ddd-4018-9857-6723082f8696", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2883, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002883__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2884-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2884, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2885, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3206", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2885", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b53d0a2-5d1f-4575-abba-38d24cb40d51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2884, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002884__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2885-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2885, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2886, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3207", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2886", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e36df74e-3b03-46ba-81bd-7758b3a301b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2885, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002885__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2886-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2886, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2887, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3208", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2887", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "777ff48b-fc30-4c5c-90ab-0cd3c26ea147", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2886, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002886__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2887-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2887, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2888, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3209", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2888", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fe64c25-5eac-406f-af91-75756cb8b848", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2887, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002887__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2888-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2888, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2889, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3210", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2889", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7412a2bc-0bbd-431b-a5b6-eae60c0e804a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2888, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002888__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2889-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2889, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2890, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3211", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2890", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2afbfcb3-cee7-4f92-bd5c-6e1799439330", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2889, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002889__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2890-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2890, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2891, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3212", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2891", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "913c22e0-4b18-46f9-a5ca-80dc896c4883", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 250986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2890, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002890__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2891-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2891, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2892, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3213", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2892", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2af16de7-0c18-48a3-bc5f-7bd6d2b27ec1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2891, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002891__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2892-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2892, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "250937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2893, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3214", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2893", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4528299-32ee-4e6f-bafa-4c98c8798030", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2892, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002892__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2893-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2893, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2894, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3215", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2894", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9afc31bb-9c15-430a-b938-465fb97af189", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2893, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002893__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2894-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2894, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2895, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3216", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2895", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0e1481d-e64b-43c5-8d50-258c0a82482d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2894, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002894__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2895-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2895, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2896, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3217", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2896", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86a67da5-b845-430e-aa28-f8d6cdd666dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2895, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002895__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2896-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2896, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2897, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3218", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2897", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "491629c0-b75b-4cf6-98f4-5e6173e32cf3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2896, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002896__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2897-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2897, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2898, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3219", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2898", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8159bb0f-24cc-428f-b223-90425ab710e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2897, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002897__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2898-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2898, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2899, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3220", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2899", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90d6efa8-2b2c-4eeb-a9ef-61278383b003", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2898, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002898__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2899-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2899, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2900, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3221", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2900", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a114851-63c3-43d0-be97-456056fb5d64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2899, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002899__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2900-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2900, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2901, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3222", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2901", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "986d3802-52a4-46e2-9c06-910a2363f95c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2900, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002900__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2901-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2901, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2902, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3223", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2902", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97cde11a-9acc-414a-96fd-badf65c727b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 251940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2901, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002901__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2902-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2902, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2903, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3224", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2903", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98bb7e45-a339-4891-894d-b94e45c3aee4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2902, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002902__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2903-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2903, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2904, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3225", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2904", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bf2f7ff-9f74-4ce6-bbd6-fd21de54c61f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2903, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002903__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2904-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2904, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "251984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2905, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3226", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2905", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b00e7258-e8a6-4e3e-be2d-e152baf19dca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2904, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002904__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2905-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2905, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2906, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3227", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2906", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c05e68f-0b68-4ef4-a5a6-c8a13d92cd00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2905, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002905__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2906-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2906, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2907, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3228", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2907", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2888c111-2ce0-4c66-85da-23e11eea9a28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2906, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002906__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2907-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2907, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2908, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3229", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2908", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d4f370a-7a3c-4f48-8870-6ae2a9bdd871", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2907, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002907__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2908-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2908, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2909, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3230", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2909", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99a3c4d1-d49b-4474-972d-98c61d3d40eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2908, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002908__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2909-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2909, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2910, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3231", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2910", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2f24881-79ed-4712-a5a9-07c31b78c2d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2909, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002909__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2910-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2910, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2911, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3232", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2911", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68a1cfb1-6c24-4346-b8a3-6912b5289e98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2910, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002910__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2911-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2911, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2912, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3233", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2912", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76eb9d5c-5da3-4a79-9e4a-f9c49f9b8adc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2911, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002911__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2912-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2912, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2913, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3234", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2913", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b2dee30-a718-4aab-ba27-6b614a3d893b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2912, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002912__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2913-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2913, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2914, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3235", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2914", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a1a47a2-0b2d-4251-9f48-f664c2389ef7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 252986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2913, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002913__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2914-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2914, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2915, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3236", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2915", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6dcaa9d1-37ae-4f51-8141-cf9c49d9c9ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2914, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002914__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2915-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2915, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "252937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2916, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3237", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2916", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcbdf805-9644-4151-81c6-056be07be288", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2915, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002915__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2916-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2916, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2917, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3238", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2917", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5924a4ea-7c41-4c1a-b58a-fdabda5f61c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2916, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002916__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2917-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2917, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2918, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3239", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2918", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18f8fe8e-6bdd-4d00-bba9-a17bc8df2a3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2917, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002917__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2918-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2918, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2919, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3240", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2919", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "614c182a-57f8-456b-9122-245ee1adabff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2918, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002918__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2919-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2919, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2920, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3241", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2920", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f73b9512-7c1b-4f65-9e10-a85c8d55f129", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2919, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002919__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2920-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2920, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2921, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3242", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2921", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d4fe672-d325-4853-b4e6-988461aa983c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2920, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002920__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2921-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2921, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2922, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3243", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2922", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "527bd404-e969-4bf3-9768-77ea5a03ed61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2921, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002921__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2922-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2922, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2923, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3244", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2923", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05f424b1-d836-473c-a7cf-de30e3467c86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2922, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002922__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2923-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2923, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2924, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3245", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2924", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0ac7f80-bdf7-4802-83e5-2b75f81a77ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2923, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002923__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2924-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2924, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2925, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3246", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2925", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ef2ca9c-9fff-495c-a724-df393035999e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 253940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2924, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002924__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2925-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2925, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2926, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3247", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2926", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07f23ef2-32bb-43d6-8065-a523ae75ea8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2925, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002925__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2926-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2926, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2927, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3248", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2927", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb611283-767c-400f-a54f-f991749a96cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2926, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002926__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2927-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2927, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "253968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2928, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3249", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2928", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "319cdf8e-b714-40c9-91a4-2a85a7084e2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2927, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002927__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2928-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2928, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2929, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:22:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3250", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2929", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e7ef764-c50a-430d-a12c-5e1d9565f470", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2928, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002928__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2929-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2929, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2930, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3251", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2930", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "729157d3-dee1-4088-9238-66e27221bbbc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2929, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002929__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2930-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2930, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2931, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3252", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2931", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "501ca30c-990b-47e8-a85b-f54d8ce101c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2930, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002930__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2931-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2931, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2932, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3253", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2932", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3f27abf-c832-43db-bfab-03a9f30e0774", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2931, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002931__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2932-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2932, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2933, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3254", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2933", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9d3feb5-8add-41b4-9fe2-df7a98a71595", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2932, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002932__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2933-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2933, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2934, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3255", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2934", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24ec2de1-1a6a-4d35-84ac-ae3d82208a0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2933, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002933__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2934-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2934, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2935, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3256", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2935", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "35039f69-76bb-4c77-a403-8b26f83ef967", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2934, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002934__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2935-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2935, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2936, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3257", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2936", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27da7db8-4c9e-49e9-8462-2ad39213f3e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2935, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002935__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2936-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2936, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2937, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3258", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2937", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b0c545e-ce24-4c6e-a377-85bcf7042adb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 254971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2936, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002936__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2937-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2937, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2938, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3259", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2938", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b738e83-1887-4d66-8d76-684c514aca16", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2937, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002937__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2938-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2938, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "254921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2939, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3260", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2939", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "427c108e-d8bd-43e9-a8dc-1df4584db43a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2938, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002938__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2939-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2939, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2940, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3261", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2940", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf569996-fe9c-4e94-8309-717810176fa3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2939, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002939__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2940-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2940, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2941, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3262", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2941", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1babff59-5384-40ad-aafe-306352a23662", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2940, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002940__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2941-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2941, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2942, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3263", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2942", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b191bfa-1ccb-401c-b870-b0ba189611a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2941, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002941__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2942-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2942, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2943, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3264", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2943", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1d5c19e-f7da-459b-9c3c-008ebd62d9a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2942, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002942__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2943-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2943, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2944, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3265", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2944", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adf046f5-386c-4bd1-9c7c-7188ba0bbff0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2943, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002943__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2944-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2944, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2945, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3266", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2945", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4559611f-8281-4420-88a9-42592a109947", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2944, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002944__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2945-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2945, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2946, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3267", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2946", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ca78553-a73d-4af9-a1ba-d82a0dc92db5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2945, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002945__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2946-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2946, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2947, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3268", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2947", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8536166f-235d-427e-a9d3-5e336e157654", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2946, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002946__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2947-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2947, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2948, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3269", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2948", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5711734-9500-4248-bc6a-27079d0d0030", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 255924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2947, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002947__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2948-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2948, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2949, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3270", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2949", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3768c131-0d49-48e6-b799-ad2edbf66efc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2948, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002948__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2949-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2949, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2950, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3271", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2950", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "839abc49-cdff-482d-b2e5-a26ca778bf40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2949, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002949__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2950-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2950, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "255968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2951, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3272", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2951", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "256b0099-50ad-4af4-ad48-db3cd78a3b8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2950, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002950__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2951-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2951, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2952, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3273", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2952", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9505609-01dd-4728-85a8-d2f6b7e70afc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2951, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002951__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2952-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2952, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2953, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3274", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2953", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cd544f9-3311-41aa-bbbe-0c5c72def692", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2952, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002952__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2953-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2953, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2954, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3275", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2954", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd85cebf-b49d-479f-bacd-96c92285aca1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2953, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002953__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2954-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2954, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2955, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3276", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2955", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fff1df7f-835d-4427-a4bc-cd510f8bcfe2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2954, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002954__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2955-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2955, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2956, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3277", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2956", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "111e677c-370e-4498-aeab-c0a9441936c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2955, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002955__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2956-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2956, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2957, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3278", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2957", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bad05c02-3d67-4ae6-8b12-4c5c07feeedf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2956, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002956__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2957-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2957, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2958, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3279", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2958", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "423597ac-1dd6-45f6-ad6e-4e8fd85c800f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2957, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002957__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2958-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2958, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2959, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3280", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2959", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9bf40ea-31ae-44b3-b2d4-8c3072f54fe9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2958, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002958__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2959-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2959, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2960, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3281", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2960", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "694c916c-ee27-4495-9e8f-92df956630b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 256971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2959, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002959__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2960-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2960, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2961, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3282", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2961", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e0e5655-d410-4453-afe6-7d9fe0be908d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2960, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002960__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2961-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2961, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "256921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2962, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3283", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2962", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebab9068-6fd9-4c57-a379-d1df6429c03e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2961, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002961__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2962-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2962, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2963, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3284", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2963", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff9afbf6-e889-4c74-9ba7-f2f50c168d06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2962, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002962__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2963-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2963, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2964, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3285", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2964", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a52f96d-8187-4f6c-9014-4fd2a9bb535c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2963, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002963__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2964-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2964, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2965, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3286", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2965", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fcb0940-19ce-493e-a6b4-55d35a7f050d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2964, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002964__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2965-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2965, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2966, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3287", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2966", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6289e6d1-6c0f-4d58-853d-15969346721c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2965, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002965__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2966-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2966, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2967, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3288", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2967", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dcee359-3edd-457f-9230-436464538317", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2966, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002966__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2967-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2967, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2968, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3289", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2968", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90ef444b-2255-49a7-a2d9-e2e57a256377", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2967, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002967__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2968-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2968, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2969, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3290", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2969", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f954b8b-8327-485f-a3f3-892ccd6d4ae8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2968, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002968__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2969-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2969, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2970, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3291", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2970", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e885777a-d846-4e16-99cc-c301c1c55ad0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2969, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002969__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2970-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2970, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2971, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3292", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2971", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99a5a0fa-d4f3-4d0a-b355-12993dbee945", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 257924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2970, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002970__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2971-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2971, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2972, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3293", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2972", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9e93ed5-e554-4ac3-a248-0ef4c9bfea23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2971, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002971__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2972-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2972, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2973, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3294", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2973", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57cd3aef-8805-4620-913e-abd0d31f0dad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2972, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002972__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2973-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2973, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "257968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2974, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3295", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2974", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c69e955-72dc-4c09-bc35-35fb9d20b20b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2973, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002973__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2974-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2974, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2975, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3296", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2975", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3919c6a9-6e08-4bc9-965b-d1d98351172c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2974, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002974__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2975-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2975, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2976, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3297", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2976", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab6452ed-1b62-4fb9-85c3-19f4a9f9bfb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2975, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002975__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2976-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2976, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2977, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3298", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2977", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59372905-4264-4878-8655-55aa672cbfc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2976, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002976__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2977-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2977, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2978, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3299", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2978", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad7fb111-0ada-46d1-81a5-bcf23b87194f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2977, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002977__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2978-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2978, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2979, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3300", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2979", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77ec3abe-c48e-4b47-867f-4dfd23c4b9ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2978, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002978__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2979-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2979, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2980, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3301", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2980", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b7e406be-0e70-44a3-bef3-999349157409", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2979, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002979__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2980-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2980, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2981, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3302", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2981", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fc5f7d2-79d2-4e11-8fad-fbbddecfaaee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2980, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002980__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2981-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2981, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2982, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3303", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2982", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58aa95fc-59e7-4baf-975d-6531fc879c2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2981, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002981__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2982-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2982, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2983, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3304", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2983", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2305a9b4-518b-4c2c-9f16-68d37ba52f2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 258971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2982, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002982__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2983-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2983, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2984, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3305", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2984", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "855e13d2-4770-4399-a8af-df9ba9b21bc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2983, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002983__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2984-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2984, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "258921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2985, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3306", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2985", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a6523b7-8e05-4996-8682-f395f777fc85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2984, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002984__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2985-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2985, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2986, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3307", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2986", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4ba3898-88d5-4d8b-b406-247a7b573b3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2985, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002985__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2986-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2986, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2987, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3308", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2987", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc22c3fd-ce53-42d1-84bf-809694718251", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2986, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002986__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2987-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2987, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2988, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3309", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2988", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1daa1494-a548-4300-bded-92f2ce92422e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2987, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002987__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2988-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2988, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2989, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3310", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2989", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "384f403f-368c-45b2-90d7-1343bf750aeb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2988, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002988__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2989-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2989, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2990, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3311", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2990", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd7cbb79-e5ef-42ee-bae5-9e717d7b3471", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2989, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002989__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2990-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2990, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2991, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3312", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2991", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f695743-3166-4415-a221-3deb1e061db7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2990, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002990__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2991-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2991, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2992, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3313", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2992", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "761939ac-de2b-4876-8041-fc42fc15b8cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2991, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002991__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2992-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2992, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2993, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3314", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2993", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b9ec000-554d-45ce-82ab-66da8a3c6946", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2992, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002992__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2993-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2993, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2994, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3315", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2994", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7baa072-9153-457c-828b-07e7c4937991", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 259924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2993, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002993__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2994-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2994, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2995, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3316", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2995", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01484dde-2a1f-46c2-a384-8e97c0d0da29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2994, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002994__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2995-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2995, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2996, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3317", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2996", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "850e3e70-a9ed-48f0-ba23-5a8a2dbc3026", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2995, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002995__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2996-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2996, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "259953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2997, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3318", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2997", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e762bfc-6ee8-4313-b7d7-ae71ed995908", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2996, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002996__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2997-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2997, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2998, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3319", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2998", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e577a353-d87b-415c-b5c5-72dd32fd2463", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2997, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002997__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2998-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2998, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 2999, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3320", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "2999", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a87bbc5d-b62e-4786-9313-f15a4fc59efe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2998, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002998__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-2999-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 2999, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3000, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3321", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3000", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebd01040-f1c2-4187-9dd6-26542bbfb28e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 2999, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000002999__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3000-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3000, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3001, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3322", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3001", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d905b7b-2596-488e-9c27-daa1b2cdf454", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3000, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3001-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3001, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3002, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3323", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3002", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1066f0a7-5929-4adb-8aef-86dc86e13e8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3001, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003001__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3002-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3002, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3003, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3324", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3003", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec79f400-4506-4f80-ab48-fd2e04393b86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3002, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003002__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3003-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3003, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3004, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3325", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3004", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc357b80-4a3b-4848-a95b-ac5af0e76028", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3003, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003003__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3004-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3004, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3005, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3326", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3005", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9aa128a6-9947-4fdb-9dd2-6957feb9fd33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3004, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003004__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3005-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3005, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3006, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3327", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3006", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b4d5ad4-1412-4b0c-bd48-0c929e5882d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 260955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3005, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003005__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3006-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3006, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3007, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3328", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3007", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff8051a2-bfcf-4701-8a93-30758bd51619", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3006, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003006__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3007-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3007, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "260906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3008, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3329", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3008", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1978b8ff-b52c-4d36-832c-1ffd06e16933", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3007, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003007__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3008-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3008, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3009, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3330", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3009", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24d51f00-3d37-42b5-8095-0883ae630ce7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3008, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003008__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3009-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3009, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3010, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3331", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3010", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "097a96e6-77ad-400d-aeb5-802bacdcac3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3009, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003009__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3010-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3010, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3011, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3332", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3011", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9003226d-92d1-47d9-9888-534ca22c2ed9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3010, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003010__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3011-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3011, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3012, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3333", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3012", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5d1ee9c-3839-49b4-923d-64ed40a70b21", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3011, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003011__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3012-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3012, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3013, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3334", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3013", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2fbf77ea-0fd6-4a9b-bf94-432986e95257", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3012, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003012__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3013-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3013, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3014, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3335", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3014", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9cebec6-f2ac-4f17-bc3a-b499e66b26ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3013, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003013__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3014-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3014, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3015, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3336", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3015", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a239f55-fc50-40be-b9ad-439b132d0b06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3014, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003014__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3015-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3015, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3016, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3337", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3016", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "680c251f-c499-499e-80bf-19c0d9ae453b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3015, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003015__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3016-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3016, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3017, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3338", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3017", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "428ead69-721e-4afc-8c45-361ce23d0cde", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 261908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3016, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003016__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3017-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3017, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3018, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3339", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3018", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df77ede6-9184-4d96-9307-af89a361e435", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3017, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003017__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3018-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3018, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3019, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3340", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3019", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a58b6ad-bc2a-4089-ab76-cc13cbf3e1ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3018, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003018__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3019-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3019, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "261953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3020, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3341", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3020", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13dec54a-df4d-4669-a415-f8ed7a079d86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3019, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003019__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3020-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3020, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3021, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3342", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3021", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f19a27df-a3e6-421f-aa47-f43a7f92ab0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3020, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003020__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3021-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3021, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3022, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3343", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3022", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d751e843-46f6-4812-943e-0c9e7f2dc8e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3021, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003021__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3022-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3022, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3023, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3344", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3023", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af949dab-7200-4cf9-9606-300a284eca82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3022, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003022__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3023-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3023, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3024, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3345", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3024", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd7a909c-5f47-4b89-8520-7c60a57dfc8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3023, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003023__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3024-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3024, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3025, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3346", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3025", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c48d127e-1808-47b5-a384-23f4948410b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3024, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003024__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3025-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3025, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3026, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3347", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3026", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34b82203-2908-48ce-a016-6be07e7e983b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3025, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003025__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3026-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3026, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3027, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3348", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3027", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ad55d53-3f47-4bd3-9750-de4bbc43d248", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3026, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003026__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3027-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3027, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3028, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3349", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3028", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40059860-d43d-4edd-a558-e3f4a6aa9998", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3027, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003027__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3028-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3028, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3029, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3350", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3029", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80812d33-cb0c-4a17-a625-b6498d185a54", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 262955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3028, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003028__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3029-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3029, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3030, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3351", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3030", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62bf56b9-f5c5-40fa-9588-c46da1dfdd2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3029, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003029__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3030-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3030, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "262906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3031, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3352", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3031", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6375b83e-7b27-4396-bc51-8a70a6d81461", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3030, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003030__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3031-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3031, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3032, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3353", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3032", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ece80bab-e1c0-48b5-87b3-550a2e8ee6b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3031, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003031__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3032-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3032, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3033, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3354", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3033", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e99b4b3-8463-4ab8-a843-274ee3d97424", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3032, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003032__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3033-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3033, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3034, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3355", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3034", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88bee78a-e76d-467b-acb9-cf290bbffe1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3033, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003033__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3034-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3034, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3035, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3356", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3035", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13111f74-0eb2-48ed-8e7b-9bb6d5727420", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3034, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003034__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3035-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3035, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3036, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3357", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3036", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e33169d5-c617-4197-98de-024fb2808eb1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3035, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003035__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3036-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3036, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3037, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3358", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3037", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c22a4e2-597f-499f-bf89-9ff18fbc67af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3036, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003036__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3037-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3037, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3038, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3359", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3038", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07ab6634-385a-4fda-a4bd-000601eb86e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3037, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003037__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3038-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3038, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3039, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3360", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3039", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baae1520-9d9c-4629-81e2-af04330253e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3038, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003038__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3039-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3039, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3040, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3361", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3040", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d40728b6-510d-4a89-b426-c0e6e70850ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 263908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3039, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003039__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3040-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3040, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3041, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3362", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3041", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f96f9def-ac61-4485-9f06-4abbe5a2d867", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3040, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003040__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3041-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3041, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3042, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3363", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3042", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7019b94a-6cf6-4e33-8c42-99b2587ebbc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3041, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003041__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3042-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3042, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "263953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3043, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3364", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3043", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd7d7b5e-f12d-4819-80d8-2f2868619871", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3042, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003042__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3043-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3043, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3044, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3365", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3044", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b550d9c8-6cc3-4d3e-8237-61d8f52cd9cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3043, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003043__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3044-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3044, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3045, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3366", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3045", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98f942ab-8a8c-4917-83e3-65eabd1c982d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3044, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003044__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3045-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3045, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3046, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3367", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3046", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fac130cb-2065-44e2-a5c3-6498132d047f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3045, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003045__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3046-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3046, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3047, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3368", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3047", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e85d2b4-ba1b-4eaf-8987-4f292e571d28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3046, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003046__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3047-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3047, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3048, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3369", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3048", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3be93b6-89ad-4475-becd-4ddf9a99b2ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3047, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003047__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3048-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3048, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3049, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3370", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3049", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3aa9233f-b9ff-4a81-8799-33413e6f9697", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3048, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003048__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3049-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3049, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3050, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3371", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3050", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9bc57e44-e624-40ac-8f1a-329f49a7165d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3049, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003049__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3050-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3050, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3051, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3372", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3051", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac8aafe3-e69b-4f92-812f-28b30eb26b5c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3050, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003050__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3051-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3051, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3052, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3373", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3052", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "984ec9d6-7c70-4993-895c-ed1344a055fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 264955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3051, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003051__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3052-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3052, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3053, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3374", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3053", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fa17fd0-698d-441a-a6c4-7aca2ef128ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3052, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003052__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3053-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3053, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3054, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3375", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3054", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "35ade441-49d2-45e1-ac3d-16e802ea07ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3053, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003053__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3054-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3054, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "264984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3055, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3376", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3055", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ac87c73-dcea-4596-9a62-5b192dec48f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3054, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003054__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3055-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3055, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3056, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3377", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3056", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3d06fb5-1f52-4820-97fd-47e8cd3a898c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3055, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003055__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3056-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3056, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3057, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3378", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3057", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d05a483-9801-48d3-ab52-182fabc81075", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3056, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003056__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3057-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3057, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3058, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3379", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3058", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "071ed8d3-62a6-42d4-8590-a04da0049c74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3057, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003057__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3058-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3058, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3059, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3380", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3059", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b314d12-29f7-4209-8221-ec07466cc028", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3058, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003058__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3059-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3059, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3060, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3381", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3060", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70f991b9-b8e7-4c55-b2fa-295878331874", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3059, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003059__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3060-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3060, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3061, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3382", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3061", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a72cf05d-cb48-49a1-b7e7-dacfcf3d08e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3060, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003060__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3061-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3061, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3062, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3383", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3062", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3134a050-8556-43cf-a53b-c71527dfbeac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3061, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003061__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3062-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3062, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3063, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3384", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3063", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "701ed021-9c52-4224-8d43-9513a3830434", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3062, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003062__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3063-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3063, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3064, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3385", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3064", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47c672dd-fa0c-4df2-a205-0d657e108827", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 265986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3063, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003063__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3064-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3064, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3065, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3386", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3065", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36b7cd64-7f3e-483a-8d8b-69c3399f4ccc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3064, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003064__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3065-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3065, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "265937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3066, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3387", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3066", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52747a1d-5788-476d-b96d-2ae18e865f8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3065, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003065__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3066-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3066, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3067, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3388", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3067", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b38240d-92ab-4636-a47b-6004f487909a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3066, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003066__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3067-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3067, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3068, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3389", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3068", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1216415-da9d-4921-8754-664029026d35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3067, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003067__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3068-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3068, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3069, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3390", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3069", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3ce500c-ec15-4341-b301-e433e59ea695", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3068, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003068__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3069-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3069, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3070, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3391", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3070", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "715d56a4-ffc1-4c17-b9bc-e93d1bea8060", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3069, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003069__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3070-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3070, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3071, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3392", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3071", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8852301-759e-4868-820a-958989c55366", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3070, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003070__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3071-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3071, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3072, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3393", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3072", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da0777a8-f5ed-4b32-8f5a-21c11024ae84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3071, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003071__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3072-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3072, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3073, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3394", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3073", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a98eca8a-5d81-4326-ba7b-2ce6d232033d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3072, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003072__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3073-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3073, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3074, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3395", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3074", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "afbe247c-7389-4272-ba53-d3471dd00405", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3073, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003073__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3074-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3074, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3075, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3396", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3075", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3394b93e-5758-49c5-a4d4-737c8a69dd4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 266940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3074, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003074__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3075-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3075, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3076, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3397", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3076", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77d077df-5f66-4d36-8c63-82c6c8357c28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3075, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003075__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3076-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3076, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3077, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3398", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3077", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbcc7e12-ac39-49b0-a544-1b99315520db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3076, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003076__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3077-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3077, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "266984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3078, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3399", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3078", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fdb8d9c2-10da-4871-a2dc-316561d6ddc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3077, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003077__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3078-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3078, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3079, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3400", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3079", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1bf7cfb-5157-4a02-94d9-4b73359e89e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3078, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003078__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3079-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3079, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3080, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3401", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3080", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bec5e31c-2367-4f5c-84c9-0c20cdd5f7c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3079, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003079__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3080-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3080, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3081, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3402", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3081", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51e25036-8060-4752-a26c-c05c49b1b7ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3080, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003080__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3081-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3081, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3082, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3403", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3082", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6aaa43e5-61ec-4376-a416-be25c511561f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3081, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003081__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3082-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3082, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3083, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3404", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3083", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2475ec1-902e-4956-9fc8-34fe421313fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3082, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003082__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3083-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3083, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3084, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3405", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3084", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0c3fc7f-72ff-4b0f-a9be-17ecd91a4522", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3083, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003083__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3084-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3084, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3085, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3406", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3085", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee4f6612-1430-4c42-83b4-850d06384398", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3084, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003084__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3085-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3085, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3086, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3407", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3086", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7fca219-a1dd-4e19-ab4f-c5502e8bc458", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3085, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003085__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3086-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3086, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3087, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3408", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3087", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64b45d88-edb2-4d1f-a614-0d112d331440", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 267986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3086, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003086__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3087-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3087, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3088, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3409", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3088", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5776093d-f58b-461d-a647-ae3f090e6835", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3087, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003087__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3088-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3088, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "267937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3089, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3410", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3089", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b88eed8c-a761-4657-a94d-feb80daa1771", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3088, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003088__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3089-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3089, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3090, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3411", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3090", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf57558c-899e-4bd7-b464-1f2f24145c2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3089, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003089__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3090-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3090, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3091, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3412", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3091", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03d26e4a-633c-4018-bce2-226a8174de01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3090, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003090__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3091-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3091, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3092, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3413", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3092", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6455a21b-4ced-43d0-93bc-181331757d79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3091, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003091__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3092-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3092, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3093, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3414", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3093", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa6faea1-a5c3-4e9a-9b85-b9c5241490da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3092, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003092__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3093-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3093, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3094, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3415", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3094", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69704cbc-0975-4c02-b52e-79f6957ec666", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3093, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003093__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3094-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3094, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3095, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3416", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3095", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01f85247-1826-44ed-b5f2-3d2cbd18bb55", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3094, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003094__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3095-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3095, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3096, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3417", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3096", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ffaed138-4709-48aa-ae04-619df1831ec3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3095, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003095__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3096-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3096, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3097, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3418", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3097", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da143284-ffe2-4043-8cf4-368d11fc5875", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3096, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003096__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3097-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3097, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3098, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3419", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3098", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6e0a6d7-b491-4e71-b1ad-3003bd5457ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 268940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3097, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003097__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3098-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3098, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3099, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3420", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3099", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b52c3b7-94b5-43b4-a31c-86f116ed5d90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3098, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003098__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3099-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3099, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3100, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3421", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3100", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a1c5b49-46ef-484c-a405-e645d13b7f9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3099, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003099__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3100-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3100, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "268984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3101, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3422", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3101", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f27dfc2-d781-4b91-82ba-ad1a43bdd742", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3100, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003100__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3101-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3101, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3102, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3423", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3102", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04c88285-b161-4da5-b1f4-41f38642be28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3101, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003101__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3102-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3102, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3103, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3424", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3103", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2429f518-5380-4d90-8fbd-08ed45631293", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3102, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003102__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3103-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3103, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3104, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3425", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3104", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36fa6e99-878e-4be2-b36f-67c71524fc01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3103, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003103__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3104-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3104, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3105, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3426", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3105", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31ef3e7c-2dd8-4f89-b140-ad5003541c13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3104, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003104__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3105-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3105, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3106, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3427", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3106", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f5c8c4d-b927-4d86-87b0-129c193cca38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3105, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003105__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3106-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3106, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3107, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3428", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3107", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f32b0db4-9f53-4fc0-a271-b2e3e18ca81e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3106, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003106__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3107-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3107, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3108, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3429", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3108", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63c243ef-038a-400f-b2df-a247c91024f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3107, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003107__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3108-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3108, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3109, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3430", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3109", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e709b600-2bad-467c-b9a0-63401bbfc966", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3108, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003108__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3109-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3109, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3110, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3431", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3110", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec25886a-9f46-4321-b92f-7f6e2716c4c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 269986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3109, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003109__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3110-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3110, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3111, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3432", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3111", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "508fdcb2-7e2b-40e2-83f0-0df142e40a3d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3110, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003110__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3111-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3111, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "269937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3112, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3433", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3112", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd0b6fac-4e02-4713-8805-5871a0b5e7f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3111, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003111__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3112-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3112, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3113, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3434", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3113", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0d2af58-2d0b-44ff-a7be-1e9fcba75df9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3112, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003112__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3113-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3113, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3114, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3435", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3114", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef7d463c-45cf-448b-a80f-59fcc24f08b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3113, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003113__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3114-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3114, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3115, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3436", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3115", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21212b3e-3588-4e39-9d70-9d8d76cf042c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3114, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003114__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3115-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3115, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3116, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3437", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3116", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94b87363-4bc7-4349-85dd-8dc4d4e5f224", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3115, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003115__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3116-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3116, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3117, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3438", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3117", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d47492e7-7148-46eb-a83d-6428cebf6d52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3116, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003116__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3117-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3117, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3118, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3439", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3118", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eacbd740-b66b-457d-95a8-d6c3c477123f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3117, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003117__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3118-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3118, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3119, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3440", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3119", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45879fc7-5733-4b3d-a7f8-1556e2925042", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3118, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003118__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3119-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3119, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3120, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3441", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3120", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "783b6147-d194-4c8e-b74c-59c7bd5bbfef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3119, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003119__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3120-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3120, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3121, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3442", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3121", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28c52420-d6d1-4d5a-b246-5d5d276f516f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 270940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3120, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003120__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3121-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3121, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3122, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3443", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3122", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1769a51-5fe1-4f95-a60a-48c83ff31368", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3121, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003121__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3122-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3122, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3123, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3444", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3123", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37e5e578-9772-4dca-91a9-468aac7029be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3122, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003122__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3123-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3123, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "270968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3124, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3445", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3124", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37bf9c54-d2d6-458b-8cc6-3ca89a305424", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3123, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003123__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3124-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3124, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3125, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3446", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3125", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2360239d-c23b-4c45-a5aa-994703a57c91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3124, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003124__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3125-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3125, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3126, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3447", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3126", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b39cfaa1-8f66-44b6-8ba6-8ad621d0303a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3125, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003125__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3126-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3126, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3127, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3448", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3127", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82b3c04c-ce44-457d-9c63-88bf32c7f104", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3126, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003126__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3127-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3127, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3128, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3449", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3128", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07d147a5-4c7a-40b4-9fb9-5034f92534ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3127, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003127__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3128-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3128, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3129, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3450", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3129", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c9262e4-0d94-45e4-a9aa-12a45eb1682f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3128, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003128__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3129-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3129, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3130, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3451", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3130", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25f4805f-72e5-446f-9cae-5867d7603609", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3129, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003129__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3130-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3130, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3131, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3452", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3131", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4ca746f-14f9-4cd2-9762-34bcbfa2ea10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3130, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003130__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3131-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3131, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3132, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3453", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3132", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f776ec14-ffa2-4daa-9d30-88c24290d0b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3131, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003131__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3132-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3132, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3133, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3454", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3133", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4375197a-e99f-4e8d-b84e-5c12a6adcbbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 271971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3132, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003132__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3133-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3133, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3134, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3455", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3134", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "562905cf-32af-45c6-8d25-3cb929e9274f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3133, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003133__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3134-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3134, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "271921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3135, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3456", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3135", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70b3ed47-9ded-44b2-82fa-59f590452027", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3134, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003134__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3135-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3135, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3136, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3457", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3136", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a46c7595-49c5-43ad-b198-9a615b264ba3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3135, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003135__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3136-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3136, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3137, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3458", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3137", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b800068-d3b4-4792-8104-4f2739c49ad7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3136, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003136__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3137-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3137, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3138, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3459", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3138", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7fa57653-2b1b-44bf-9941-854ab0e644fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3137, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003137__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3138-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3138, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3139, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3460", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3139", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d24804e9-daf4-42bb-9916-663337de064c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3138, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003138__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3139-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3139, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3140, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3461", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3140", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83a967a0-4783-4239-99f0-26008f922add", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3139, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003139__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3140-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3140, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3141, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3462", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3141", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d0eb9d5-070c-451c-bf30-1140afbf1bab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3140, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003140__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3141-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3141, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3142, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3463", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3142", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db7c58cb-09c7-4633-9058-1db21e0d1fc5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3141, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003141__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3142-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3142, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3143, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3464", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3143", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1b90168-116c-4321-ad67-e9b8d0824637", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3142, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003142__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3143-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3143, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3144, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3465", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3144", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "898d4027-5bdc-4c71-b799-bc5a4b57659e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 272924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3143, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003143__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3144-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3144, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3145, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3466", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3145", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf99cdd8-d3e7-4689-ac7a-c0f4f70fc7d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3144, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003144__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3145-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3145, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3146, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3467", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3146", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec82cd28-916a-4787-85af-9107422334b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3145, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003145__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3146-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3146, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "272968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3147, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3468", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3147", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e7476e82-5e73-4be4-bef0-17239ca5622c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3146, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003146__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3147-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3147, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3148, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3469", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3148", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b077013f-52de-4ad9-9e4a-e878ebafc640", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3147, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003147__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3148-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3148, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3149, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3470", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3149", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2dd8216b-f89a-4f7b-8d44-3adc287060ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3148, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003148__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3149-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3149, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3150, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3471", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3150", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32398e12-328f-4046-968b-b91a45d91085", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3149, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003149__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3150-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3150, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3151, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3472", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3151", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d18ab8c5-967c-45ce-9985-78181a0b5b66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3150, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003150__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3151-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3151, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3152, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3473", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3152", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f01eb90b-e789-44d4-a967-4fcc80a525e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3151, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003151__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3152-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3152, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3153, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3474", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3153", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa544972-0732-4113-b1ac-c0dcf44b3740", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3152, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003152__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3153-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3153, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3154, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3475", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3154", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ec07ff2-2f4a-4d7c-82d2-08319b08168d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3153, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003153__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3154-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3154, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3155, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3476", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3155", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e6d1f19-21be-4c77-aa62-0b236644d285", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3154, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003154__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3155-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3155, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3156, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3477", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3156", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3774919f-7718-43c8-b4b4-81ac71281c2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 273971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3155, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003155__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3156-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3156, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3157, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3478", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3157", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7af04e8-da8f-4e9a-86a6-58a2a8b3b39a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3156, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003156__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3157-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3157, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "273921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3158, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3479", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3158", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "441a160d-02ca-4ec6-8f9d-574637ca8298", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3157, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003157__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3158-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3158, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3159, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3480", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3159", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca0c72b3-a821-4d2d-a5b5-bed56d90d4b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3158, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003158__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3159-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3159, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3160, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3481", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3160", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36416402-c732-4d33-a69f-226333b5fecd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3159, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003159__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3160-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3160, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3161, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3482", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3161", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59b41265-90a4-4c98-8d4b-bd71c94665cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3160, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003160__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3161-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3161, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3162, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3483", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3162", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17128797-4e11-4270-a8a3-df5568089399", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3161, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003161__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3162-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3162, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3163, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3484", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3163", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33129bff-ecd4-4d4a-a8f9-2b6d24c44a1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3162, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003162__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3163-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3163, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3164, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3485", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3164", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "033aad01-2824-4300-8012-2789a7b770ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3163, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003163__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3164-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3164, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3165, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3486", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3165", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baba2b91-0eed-4969-b2b6-00e012ddc45b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3164, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003164__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3165-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3165, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3166, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3487", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3166", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8550e479-baae-497f-9545-e1608a4e6ed9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3165, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003165__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3166-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3166, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3167, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3488", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3167", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2520ef92-6cfa-4471-a2b0-dbce22edf7db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 274924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3166, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003166__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3167-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3167, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3168, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3489", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3168", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3f6cfea-4484-4e8b-8bff-79e0e2b58fc3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3167, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003167__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3168-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3168, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3169, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3490", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3169", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6eb0d841-3d31-429a-a41c-ea6204bc457c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3168, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003168__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3169-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3169, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "274968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3170, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3491", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3170", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b5d6537-80fa-4372-b88f-b580591ed806", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3169, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003169__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3170-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3170, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3171, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3492", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3171", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d3073e9-6691-4450-b5db-c96a30ee1f9c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3170, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003170__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3171-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3171, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3172, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3493", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3172", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58cd1546-91b2-4657-9b0e-288dc874ab5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3171, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003171__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3172-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3172, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3173, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3494", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3173", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1048774-404a-48e4-a970-0c88693ece79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3172, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003172__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3173-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3173, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3174, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3495", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3174", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f81c44ad-cd89-4aa4-a4be-5f8b71cfa52d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3173, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003173__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3174-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3174, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3175, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3496", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3175", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2487ab50-3164-41e0-b0b4-0242b282d9da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3174, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003174__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3175-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3175, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3176, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3497", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3176", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0b6b543-e93c-4925-85e2-f5dc0747b93d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3175, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003175__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3176-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3176, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3177, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3498", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3177", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a79c1ec-d2e0-4d7f-8eae-41babf412264", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3176, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003176__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3177-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3177, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3178, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3499", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3178", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1c6104d-e8ee-4a62-94a2-7051fcc50b65", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3177, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003177__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3178-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3178, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3179, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3500", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3179", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "addc29d1-4f33-453a-9dc5-0fab34224f52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3178, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003178__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3179-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3179, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3180, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3501", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3180", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6404749-74ce-42b7-919d-b390ad642ff3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3179, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003179__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3180-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3180, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "275921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3181, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3502", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3181", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99fbc9c7-6c79-4fb3-92cd-e38d2183635c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3180, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003180__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3181-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3181, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3182, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3503", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3182", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12418780-0385-4f2c-893a-0f4bef799088", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3181, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003181__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3182-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3182, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3183, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3504", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3183", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e5398a2-2525-4d84-ae82-d67d2fcd90c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3182, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003182__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3183-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3183, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3184, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3505", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3184", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef5d0149-02ba-417a-885a-5b61abff15f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3183, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003183__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3184-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3184, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3185, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3506", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3185", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5bd0b25-7cc3-454f-aed0-cc90eef4c215", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3184, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003184__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3185-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3185, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3186, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3507", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3186", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4745a04a-44c3-4bdb-bd86-54338f973cc9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3185, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003185__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3186-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3186, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3187, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3508", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3187", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9aba8722-4d71-494f-a8c3-070eaf258894", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3186, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003186__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3187-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3187, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3188, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3509", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3188", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d7c3781-adc6-48b2-9f2b-b98a5a499b1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3187, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003187__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3188-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3188, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3189, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3510", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3189", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b3c8aad-41cb-41c0-9ea9-3a75c348aad4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3188, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003188__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3189-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3189, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3190, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3511", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3190", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae5344a4-36c7-406b-8872-1080d38d476d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 276924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3189, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003189__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3190-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3190, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3191, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3512", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3191", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c6313b9-957a-4b91-9d2e-060b4d18f59e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3190, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003190__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3191-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3191, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3192, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3513", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3192", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ec4ecc3-3cb4-433d-8ed2-5951c19fbaa7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3191, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003191__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3192-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3192, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "276953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3193, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3514", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3193", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da666c7f-5cff-4c5a-a781-310624b692c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3192, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003192__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3193-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3193, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3194, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3515", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3194", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69ab0b56-8f90-42ff-b38d-e9f4d9d6b848", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3193, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003193__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3194-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3194, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3195, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3516", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3195", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4dd55f6-465b-44cb-b26e-63238aa5c698", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3194, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003194__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3195-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3195, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3196, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3517", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3196", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0b128b3-c2d4-4114-9aab-e3281edfd718", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3195, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003195__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3196-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3196, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3197, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3518", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3197", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2f3a5ee-cfcd-486d-8be9-2a0bd7d48c03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3196, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003196__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3197-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3197, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3198, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3519", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3198", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bbbbe20-62ba-42ee-8a11-02bc1efff001", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3197, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003197__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3198-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3198, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3199, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3520", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3199", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b06a403-c0ef-4ec0-bb9d-9f2d17cfc050", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3198, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003198__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3199-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3199, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3200, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3521", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3200", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0437088-787c-485f-8784-9fc71a8edbc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3199, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003199__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3200-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3200, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3201, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3522", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3201", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03739c74-35b5-4554-a03d-5a7c2c6d0c88", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3200, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003200__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3201-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3201, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3202, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3523", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3202", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a274b7c-35da-41fd-a700-ad5a9a9d852f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 277955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3201, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003201__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3202-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3202, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3203, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3524", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3203", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b1e3b7a-3e9a-41fd-b634-fc317ee0ac84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3202, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003202__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3203-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3203, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "277906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3204, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3525", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3204", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebdb4b19-4f54-4090-92f2-a8aff1c45c51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3203, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003203__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3204-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3204, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3205, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3526", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3205", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90e80335-2a39-4bb9-9ac3-1dd78cf31f7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3204, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003204__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3205-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3205, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3206, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3527", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3206", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e08e42e-7248-475b-b2fe-dbd5e41715d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3205, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003205__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3206-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3206, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3207, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3528", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3207", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38c376a6-dcf1-461c-89ef-ea45be3be3ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3206, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003206__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3207-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3207, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3208, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3529", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3208", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae455316-0992-49a5-a9bd-5ef1bf3b9ad4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3207, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003207__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3208-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3208, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3209, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3530", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3209", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3937a9cd-67cf-4243-9185-401f5932689c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3208, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003208__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3209-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3209, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3210, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3531", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3210", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a09bbf6-5406-4d04-9316-9f0ae71218d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3209, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003209__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3210-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3210, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3211, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3532", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3211", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e01eab37-c65d-4db0-b00f-6b9764e3ff18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3210, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003210__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3211-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3211, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3212, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3533", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3212", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec70d154-e893-4482-811a-669085711bf9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3211, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003211__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3212-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3212, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3213, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3534", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3213", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e00e9c3-7b9c-4bd1-bafc-7d11c6823257", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 278908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3212, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003212__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3213-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3213, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3214, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3535", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3214", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c8a7dac-ccb1-4b7f-9d84-fef745583a5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3213, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003213__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3214-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3214, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3215, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3536", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3215", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4451340f-1375-4846-a3b4-1d71b4d3a826", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3214, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003214__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3215-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3215, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "278953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3216, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3537", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3216", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0116b19-0f49-4edd-8110-4f8b1e949f75", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3215, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003215__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3216-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3216, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3217, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3538", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3217", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc9f5115-a5c5-496e-8fc2-fee657ce1dd6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3216, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003216__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3217-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3217, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3218, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3539", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3218", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89bd27b1-aedc-4375-9fd4-5a71464ce8aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3217, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003217__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3218-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3218, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3219, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3540", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3219", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6f14bda-f2d0-43cd-b474-b95e5b1632e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3218, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003218__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3219-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3219, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3220, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3541", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3220", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de44a539-2569-41aa-9e8c-7e7439da4c4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3219, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003219__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3220-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3220, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3221, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3542", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3221", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1419404-2549-4f63-9220-8a4e50ac9928", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3220, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003220__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3221-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3221, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3222, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3543", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3222", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3792c409-d58b-439a-a660-b62135e98148", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3221, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003221__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3222-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3222, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3223, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3544", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3223", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60d4d1c2-9de6-49cc-bd98-95bb547b2003", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3222, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003222__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3223-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3223, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3224, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3545", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3224", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44af61b0-2a3a-4209-bef1-80c480b90935", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3223, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003223__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3224-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3224, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3225, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3546", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3225", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c928980d-62c4-4759-a362-17201881240c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 279955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3224, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003224__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3225-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3225, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3226, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3547", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3226", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ddcf3c63-5427-4e4e-8f98-79a28d0f60e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3225, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003225__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3226-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3226, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "279906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3227, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3548", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3227", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba1cb5c7-8323-4086-b601-aac4db1b0f44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3226, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003226__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3227-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3227, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3228, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3549", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3228", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ee8d9a2-002f-4332-9d05-bd945eb8bf97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3227, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003227__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3228-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3228, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3229, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3550", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3229", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "722c35f0-4c54-4e65-a012-d37fa1d8ddb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3228, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003228__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3229-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3229, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3230, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3551", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3230", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c308719c-dea0-4e2b-85d1-ca77a77c46be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3229, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003229__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3230-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3230, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3231, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3552", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3231", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3a15035-f2a3-4f80-8f71-2a6c435b3a78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3230, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003230__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3231-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3231, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3232, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3553", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3232", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4222502a-f093-4ccd-9d38-6d0abc423c08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3231, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003231__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3232-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3232, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3233, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3554", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3233", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94fcddb5-3105-419c-aaff-ebd265829e85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3232, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003232__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3233-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3233, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3234, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3555", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3234", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb09f934-efa7-45be-b31b-93981296fad1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3233, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003233__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3234-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3234, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3235, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3556", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3235", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a95512ef-91d4-450c-b4a7-9ab6b6dcf5e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3234, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003234__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3235-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3235, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3236, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3557", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3236", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83ac6bb7-cf6b-45cb-819d-8f9e5e1f3ab6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 280908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3235, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003235__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3236-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3236, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3237, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3558", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3237", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d63a19dc-a0e7-4bdf-bea4-7acbc46329b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3236, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003236__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3237-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3237, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3238, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3559", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3238", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "227e8cd4-8d42-4089-bdf1-4993f90acca4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3237, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003237__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3238-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3238, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "280953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3239, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3560", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3239", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de4d80c9-832d-46a1-bee6-bb466bbe5b5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3238, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003238__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3239-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3239, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3240, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3561", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3240", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc1f6cda-d189-4375-adef-ee5eaec1e00d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3239, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003239__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3240-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3240, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3241, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3562", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3241", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f93f9b32-9c94-4823-8373-1317fd3a6e52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3240, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003240__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3241-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3241, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3242, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3563", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3242", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5901e48e-15ed-4a5e-8bc0-d6c1489f840a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3241, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003241__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3242-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3242, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3243, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3564", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3243", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba9da861-3857-4243-b8f2-ccb646c8f053", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3242, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003242__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3243-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3243, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3244, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3565", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3244", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd0181de-affb-4b75-a533-dc791d2d9021", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3243, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003243__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3244-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3244, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3245, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3566", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3245", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20d4619c-e2b9-464f-bedc-2d5ec15475e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3244, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003244__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3245-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3245, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3246, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3567", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3246", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "410078e0-a334-46f9-a9b5-d92122dd1443", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3245, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003245__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3246-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3246, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3247, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3568", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3247", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9196242d-1da7-4250-b24e-9355030e5968", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3246, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003246__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3247-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3247, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3248, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3569", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3248", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0d7dc5e-9a8d-48ac-a613-1d9fd1efe2c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 281955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3247, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003247__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3248-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3248, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3249, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3570", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3249", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd58fb38-e34c-4cad-b85b-27fa6c66e44b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3248, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003248__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3249-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3249, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3250, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3571", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3250", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e93768c3-dfbe-45b3-8425-01e28548f203", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3249, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003249__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3250-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3250, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "281984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3251, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3572", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3251", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f02f95fb-b4e8-43dc-8543-f296eb5fa0e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3250, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003250__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3251-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3251, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3252, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3573", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3252", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9293d70c-ba45-4b66-ad85-7c617d49bcde", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3251, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003251__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3252-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3252, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3253, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3574", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3253", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "552f9538-7b7f-459d-8e03-4fb9c52d3fdc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3252, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003252__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3253-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3253, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3254, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3575", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3254", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "027585ab-a322-4efa-a67d-0a5a4b910aa0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3253, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003253__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3254-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3254, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3255, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3576", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3255", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8fac2b0-d7be-44d9-9a81-1b84f004ea9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3254, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003254__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3255-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3255, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3256, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3577", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3256", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "146fb237-fcbd-4315-82aa-779c53089b53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3255, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003255__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3256-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3256, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3257, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3578", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3257", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe69f41b-b362-49c9-a453-5ff857db4424", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3256, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003256__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3257-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3257, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3258, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3579", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3258", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d628c1eb-6bc8-43f4-a6d9-18a2de44d573", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3257, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003257__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3258-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3258, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3259, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3580", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3259", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a78106bb-a314-4981-bc60-edf5d276a03b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3258, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003258__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3259-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3259, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3260, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3581", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3260", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d88a1187-b199-4b1d-9cb5-2706050046d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 282986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3259, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003259__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3260-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3260, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3261, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3582", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3261", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04555bf4-534e-4218-925c-d5ec540f15d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3260, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003260__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3261-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3261, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "282937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3262, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3583", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3262", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "854d0a20-49c9-45d8-bbc6-fb615500ae2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3261, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003261__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3262-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3262, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3263, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3584", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3263", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fb90460-aa0a-46cc-80fc-84624e91a55d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3262, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003262__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3263-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3263, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3264, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3585", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3264", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29326d85-fb48-4954-a7bc-67bc14b694f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3263, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003263__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3264-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3264, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3265, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3586", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3265", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f284d2f-8a91-4b78-b5ae-168a3e40a971", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3264, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003264__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3265-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3265, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3266, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3587", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3266", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a41fa751-2561-4b1e-90d7-fd260b679907", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3265, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003265__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3266-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3266, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3267, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3588", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3267", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fdbce278-8bc9-410a-9735-7ec62ea4f78f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3266, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003266__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3267-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3267, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3268, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3589", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3268", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b0a0124-ec45-40a7-9064-aee7271657ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3267, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003267__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3268-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3268, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3269, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3590", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3269", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d1d34a9-8205-4ab4-85ad-da3e158a4914", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3268, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003268__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3269-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3269, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3270, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3591", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3270", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "497d4b1c-9bbe-43b1-bc52-0407d8942bf9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3269, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003269__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3270-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3270, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3271, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3592", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3271", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48880cc4-eac2-4056-b488-5d9e86252593", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 283940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3270, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003270__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3271-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3271, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3272, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3593", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3272", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75d113bc-21fa-45f1-a1e7-7aa4eb718980", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3271, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003271__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3272-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3272, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3273, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3594", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3273", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d474bba-b656-443c-9234-1e376f1ead9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3272, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003272__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3273-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3273, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "283984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3274, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3595", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3274", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db3236eb-6a3f-44d5-8a3c-4c3aaf2936b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3273, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003273__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3274-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3274, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3275, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3596", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3275", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be9ae293-8493-4b0d-ab21-02ce8fac18a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3274, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003274__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3275-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3275, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3276, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3597", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3276", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "912236ea-88c9-4834-83b3-3a149f6d9cb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3275, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003275__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3276-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3276, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3277, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3598", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3277", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7e0eda1-1e40-4cbb-8cc0-cfe0af5f625f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3276, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003276__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3277-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3277, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3278, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3599", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3278", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f0b6ca3-982f-43c7-a304-f71cefb5a260", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3277, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003277__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3278-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3278, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3279, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3600", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3279", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a532598-2dad-4fde-93cc-97bd033f96f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3278, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003278__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3279-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3279, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3280, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3601", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3280", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecac1b2c-891d-4910-a932-72684b99d2a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3279, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003279__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3280-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3280, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3281, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3602", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3281", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3d96e4d-2286-4b3e-81b5-7af86a20710f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3280, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003280__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3281-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3281, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3282, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3603", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3282", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ea96c93-8979-4cb2-a1eb-45901754aa6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3281, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003281__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3282-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3282, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3283, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3604", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3283", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f5409da-a29f-4596-bf57-9520cf40110d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 284986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3282, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003282__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3283-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3283, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3284, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3605", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3284", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8882cdc0-43f1-4637-bf6c-996893ddd783", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3283, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003283__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3284-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3284, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "284937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3285, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3606", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3285", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dee4536c-c012-477e-9b65-213b350386ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3284, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003284__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3285-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3285, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3286, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3607", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3286", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2960cade-9bc5-4fda-b00e-0d38a0c2c758", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3285, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003285__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3286-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3286, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3287, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3608", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3287", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61042393-c9f1-44a5-b6da-e9101119860d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3286, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003286__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3287-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3287, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3288, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3609", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3288", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bc0b362-ae5f-448b-807f-8d2e82b40e74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3287, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003287__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3288-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3288, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3289, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3610", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3289", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "276427f8-dd5b-4bd7-918f-70877cd97135", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3288, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003288__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3289-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3289, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3290, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3611", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3290", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34e1c228-306d-48ef-8fa3-b860613314d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3289, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003289__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3290-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3290, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3291, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3612", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3291", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02b043a2-218d-421c-a676-a820e7c94e3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3290, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003290__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3291-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3291, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3292, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3613", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3292", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df17dc4c-96e0-4788-a3f4-3304b464424a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3291, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003291__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3292-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3292, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3293, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3614", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3293", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec536145-26da-4cc3-8ae4-80cccfd9aebb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3292, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003292__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3293-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3293, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3294, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3615", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3294", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "164e036e-ece6-46be-8d55-354e8ccac5d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 285940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3293, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003293__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3294-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3294, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3295, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3616", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3295", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fea37b8-797c-47e3-88da-fc4d624f4b2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3294, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003294__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3295-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3295, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3296, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3617", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3296", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d74d95e8-be2b-4ecc-8eb0-7ac4e9664249", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3295, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003295__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3296-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3296, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "285984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3297, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3618", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3297", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d053c450-1111-458f-b991-a27d240d915a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3296, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003296__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3297-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3297, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3298, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3619", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3298", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00469158-0fae-4c83-a985-4fcedf9b42eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3297, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003297__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3298-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3298, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3299, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3620", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3299", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb7efadd-b29f-42f0-89ca-52c5e7d50922", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3298, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003298__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3299-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3299, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3300, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3621", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3300", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5574059e-a48e-4e66-ac30-57a9d128e5a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3299, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003299__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3300-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3300, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3301, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3622", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3301", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0e9a0f5-98df-4369-9b3c-be186bc905a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3300, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003300__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3301-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3301, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3302, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3623", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3302", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09bd0037-2400-40b6-a9fb-7f5ae6d80ae4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3301, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003301__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3302-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3302, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3303, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3624", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3303", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05acfa77-6aac-4510-818d-63693cfc5982", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3302, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003302__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3303-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3303, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3304, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3625", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3304", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26871a1c-fbd6-48b3-bf6a-03b1cc4e92c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3303, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003303__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3304-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3304, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3305, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3626", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3305", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ec06a8c-8d6b-4f1e-8879-33fa946c072e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3304, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003304__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3305-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3305, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3306, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3627", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3306", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4171e566-3c9e-4bd9-9fc8-c2c7095b08c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 286986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3305, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003305__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3306-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3306, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3307, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3628", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3307", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc730669-c2c7-4ad8-a546-83944ec2deea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3306, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003306__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3307-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3307, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "286937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3308, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3629", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3308", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6b3a386-bc52-4023-bf19-b45e27030681", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3307, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003307__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3308-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3308, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3309, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3630", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3309", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c57c389e-2adc-49de-84e3-9b2bb4746e8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3308, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003308__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3309-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3309, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3310, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3631", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3310", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cabbf9f6-7db6-4123-9bd6-93398e357761", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3309, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003309__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3310-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3310, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3311, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3632", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3311", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1db2312f-e7f0-4f86-8143-176470f37a2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3310, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003310__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3311-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3311, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3312, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3633", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3312", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c57e6e37-a196-40a6-a2f4-b508886d438b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3311, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003311__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3312-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3312, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3313, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3634", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3313", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b8eb98d-5dc2-4284-8147-53b5205a244e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3312, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003312__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3313-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3313, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3314, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3635", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3314", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50085099-3abe-4684-9f74-0edb9b46cad2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3313, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003313__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3314-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3314, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3315, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3636", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3315", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0eb740d5-dfa2-4a24-9711-34ca16ddc317", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3314, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003314__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3315-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3315, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3316, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3637", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3316", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6dfe43e6-d5ba-47a4-a634-ffd88501c299", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3315, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003315__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3316-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3316, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3317, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3638", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3317", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d853de8-471c-4007-8714-0f58a1c1e026", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 287940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3316, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003316__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3317-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3317, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3318, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3639", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3318", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3de69ccc-fffb-486e-916b-4d75e0fffd7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3317, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003317__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3318-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3318, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3319, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3640", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3319", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0817b85-a8bf-4f8f-a97b-df36d393ab9c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3318, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003318__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3319-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3319, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "287968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3320, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3641", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3320", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60b81821-7b9c-4ea2-8048-5ca9c8743f13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3319, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003319__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3320-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3320, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3321, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3642", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3321", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d9a5574-9f86-49d2-90f7-3372104f8fd6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3320, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003320__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3321-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3321, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3322, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3643", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3322", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7cd2692-5618-4d3e-8cc2-1955116b5884", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3321, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003321__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3322-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3322, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3323, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3644", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3323", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8024253b-a3ca-44d4-b825-7e165ac6a0d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3322, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003322__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3323-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3323, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3324, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3645", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3324", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf705793-8728-4e8d-bad6-4b73fde33bab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3323, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003323__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3324-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3324, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3325, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3646", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3325", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0376b1c5-5595-4412-b3ba-d12d08753376", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3324, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003324__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3325-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3325, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3326, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3647", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3326", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf87ca39-df39-4441-98e5-2ad91c22e3a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3325, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003325__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3326-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3326, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3327, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3648", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3327", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "136d17e9-644c-4c05-9f08-ee6c7b2ac289", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3326, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003326__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3327-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3327, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3328, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3649", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3328", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fc7de6e-2afb-4aba-8d46-73d5c974d056", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3327, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003327__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3328-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3328, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3329, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3650", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3329", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f3d6a6f-c3ea-46c9-af2b-1a7cb0bd7fa2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 288971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3328, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003328__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3329-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3329, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3330, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3651", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3330", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec5e395e-8bc1-48a0-b3d8-61f629476228", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3329, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003329__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3330-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3330, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "288921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3331, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3652", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3331", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "295702d7-cdbc-4560-91e3-247e9e0bb7f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3330, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003330__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3331-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3331, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3332, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3653", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3332", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b482f26-8bca-4c13-b468-5bfc9c2e1cc5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3331, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003331__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3332-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3332, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3333, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3654", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3333", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "998d62b6-4114-4e6d-a6a8-c0390a65afbf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3332, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003332__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3333-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3333, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3334, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3655", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3334", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e671d7e-cf37-4c87-9938-f7b1ae76c671", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3333, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003333__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3334-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3334, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3335, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3656", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3335", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa874ccd-b05a-473e-87e1-543c4c1d6de8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3334, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003334__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3335-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3335, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3336, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3657", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3336", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53530dce-f958-41d1-98ac-5f1aaed6e50b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3335, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003335__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3336-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3336, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3337, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3658", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3337", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea153d1a-09a3-45ef-8eff-aca6aec93608", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3336, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003336__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3337-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3337, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3338, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3659", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3338", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2c6f1cc-5483-4728-91e3-7fe2707517cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3337, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003337__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3338-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3338, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3339, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3660", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3339", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f258714b-0e63-40b6-a092-a684d3579811", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3338, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003338__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3339-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3339, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3340, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3661", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3340", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f0ccb12-8afd-4fe9-b858-26545edad700", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 289924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3339, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003339__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3340-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3340, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3341, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3662", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3341", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04f61181-0722-4c5e-ae96-006c4bf4d7d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3340, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003340__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3341-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3341, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3342, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3663", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3342", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2469476-0a2e-4b8c-8ab9-d4143c02ffc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3341, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003341__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3342-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3342, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "289968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3343, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3664", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3343", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e00b7d5f-f83b-4cf4-bdfe-8af450d20b13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3342, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003342__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3343-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3343, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3344, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3665", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3344", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16c06a71-e3c4-4ac0-981e-a93ac45856e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3343, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003343__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3344-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3344, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3345, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3666", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3345", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4e4203d-ea51-450f-828d-c636d0dc9e9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3344, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003344__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3345-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3345, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3346, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3667", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3346", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e0beb1d-f8f1-4f32-923c-ccddc8066a01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3345, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003345__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3346-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3346, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3347, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3668", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3347", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77b7cb66-4ca9-41ea-b449-e885a6bfcdab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3346, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003346__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3347-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3347, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3348, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3669", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3348", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abb07310-645e-4b85-b8de-225f4c857801", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3347, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003347__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3348-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3348, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3349, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3670", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3349", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "198fb412-035c-4601-adad-43422ab87824", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3348, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003348__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3349-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3349, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3350, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3671", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3350", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "469206ef-94f5-4410-a7ed-2055b29216c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3349, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003349__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3350-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3350, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3351, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3672", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3351", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d954028-f587-43a6-8d11-132d7c55a39a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3350, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003350__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3351-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3351, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3352, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3673", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3352", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "037deb9e-d912-4572-a185-75f6aad1c569", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 290971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3351, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003351__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3352-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3352, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3353, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3674", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3353", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28bec5be-2f68-446e-b00d-d35a4065e604", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3352, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003352__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3353-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3353, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "290921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3354, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3675", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3354", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea979fc8-cdc7-4529-b541-bbc606c946f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3353, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003353__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3354-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3354, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3355, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3676", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3355", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8209549-061b-4d3d-8353-f53779fbfa3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3354, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003354__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3355-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3355, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3356, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3677", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3356", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99ca95a7-f4c9-4741-803a-edeee8be5d84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3355, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003355__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3356-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3356, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3357, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3678", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3357", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6f64715-94d9-4bc6-a38e-8a5925adfdec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3356, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003356__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3357-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3357, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3358, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3679", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3358", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82bba64e-715d-4de7-9ab0-3388b1b2d83e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3357, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003357__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3358-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3358, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3359, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3680", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3359", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb907554-3cbc-4211-b371-a95d3e1995a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3358, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003358__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3359-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3359, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3360, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3681", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3360", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9d2d3ba-3966-4be5-8f17-9050e6253a8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3359, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003359__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3360-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3360, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3361, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3682", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3361", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9693c3e-2a43-4ed6-b734-ca3c911ff75d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3360, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003360__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3361-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3361, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3362, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3683", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3362", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e09af84b-9169-4e08-88c3-954138112377", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3361, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003361__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3362-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3362, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3363, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3684", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3363", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cecf593-ae9b-4d5c-9ebe-4d2b2a1b7d0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 291924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3362, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003362__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3363-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3363, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3364, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3685", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3364", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10542450-ace9-4362-8272-cbda8612656b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3363, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003363__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3364-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3364, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3365, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3686", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3365", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8ee9167-f7d2-4e58-96ce-9313f534f677", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3364, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003364__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3365-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3365, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "291968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3366, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3687", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3366", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3087fcb7-b29c-46d9-b04f-666685769c03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3365, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003365__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3366-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3366, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3367, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3688", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3367", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e16b035c-8761-4ae9-9733-eff7a43ee9ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3366, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003366__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3367-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3367, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3368, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3689", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3368", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15965bed-9d78-429b-ac7f-e255b775f635", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3367, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003367__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3368-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3368, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3369, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3690", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3369", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7dd883ca-605a-4c2a-9b9a-3f60f880f4f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3368, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003368__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3369-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3369, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3370, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3691", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3370", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba3f1da7-e843-4b7a-b7ed-652c889f4134", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3369, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003369__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3370-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3370, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3371, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3692", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3371", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd2fba4c-5349-446f-b954-87c5177778c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3370, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003370__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3371-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3371, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3372, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3693", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3372", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b599dfd0-e616-4b8d-b2de-6cc388300bc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3371, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003371__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3372-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3372, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3373, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3694", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3373", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2eefbb6c-cd32-4a15-9e6c-e95fa17054e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3372, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003372__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3373-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3373, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3374, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3695", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3374", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ef3a3e0-2ceb-4949-8308-b6a187d0820d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3373, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003373__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3374-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3374, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3375, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3696", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3375", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9ce6272-8854-46a0-a4e5-b61f927fd635", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 292971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3374, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003374__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3375-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3375, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3376, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3697", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3376", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ae70031-06cd-40bf-873f-b7a4323c2fed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3375, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003375__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3376-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3376, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "292921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3377, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3698", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3377", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9212b22-0ed9-4954-b53c-c34c22a125ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3376, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003376__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3377-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3377, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3378, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3699", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3378", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64d94cce-faa3-4b86-a289-381592a62acb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3377, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003377__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3378-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3378, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3379, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3700", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3379", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53294198-3bbb-400a-b972-00916107b95d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3378, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003378__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3379-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3379, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3380, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3701", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3380", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e4c1cfb-884b-4e17-a074-fd4349918710", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3379, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003379__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3380-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3380, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3381, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3702", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3381", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "934bc2a3-2845-425a-81ae-17ae7fc1146a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3380, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003380__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3381-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3381, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3382, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3703", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3382", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b9b2791-1316-45f7-94b8-86531f567c01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3381, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003381__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3382-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3382, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3383, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3704", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3383", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2478b3c3-4e7c-4172-aca8-095ff101b084", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3382, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003382__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3383-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3383, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3384, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3705", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3384", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9df2b44f-58fb-48d5-bd8c-6db6d41c84ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3383, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003383__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3384-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3384, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3385, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3706", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3385", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ee8e2df-1882-4a15-8610-987b98fb763e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3384, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003384__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3385-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3385, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3386, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3707", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3386", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78827c02-8ef9-4737-989b-b448cc28748d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 293924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3385, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003385__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3386-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3386, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3387, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3708", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3387", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "717a6572-449b-4267-ac9b-f766b4a7ef81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3386, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003386__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3387-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3387, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3388, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3709", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3388", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80066100-32dd-448d-a46d-e7eda67ed059", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3387, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003387__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3388-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3388, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "293953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3389, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3710", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3389", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "064ed428-6786-4aa0-bb19-5ba27f031b4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3388, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003388__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3389-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3389, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3390, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3711", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3390", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c1a3ca7-4de2-4de9-a3a9-790fec5c3e8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3389, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003389__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3390-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3390, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3391, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3712", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3391", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c72ca084-d98b-49d2-b8b5-af100b54e60c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3390, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003390__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3391-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3391, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3392, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3713", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3392", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b53e9d2f-c932-45bb-a235-e5c58d43ea3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3391, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003391__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3392-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3392, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3393, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3714", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3393", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecfe92dd-1255-427a-b5cf-51ac30e8f8b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3392, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003392__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3393-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3393, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3394, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3715", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3394", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2cd612f1-6d8e-4928-9a4c-ed1519c3dce7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3393, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003393__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3394-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3394, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3395, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3716", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3395", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "259684a2-4bbc-4f6e-8f2e-4fef1e42d879", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3394, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003394__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3395-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3395, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3396, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3717", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3396", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5698003-a7d8-4f79-8739-d8308da1c4ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3395, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003395__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3396-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3396, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3397, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3718", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3397", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "901f5901-dadf-4e50-9d9f-d4a79e91ce7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3396, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003396__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3397-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3397, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3398, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3719", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3398", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76fe5ef6-b55b-42db-a766-07877a889fe0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 294955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3397, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003397__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3398-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3398, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3399, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3720", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3399", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d76f0b1-1e8f-4459-bb4e-75a304d82c76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3398, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003398__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3399-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3399, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "294921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3400, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3721", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3400", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8b539f2-7636-4e93-9eee-0bac18594ca4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3399, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003399__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3400-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3400, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3401, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3722", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3401", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bada07cb-1225-406f-b03e-f89f14c49996", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3400, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003400__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3401-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3401, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3402, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3723", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3402", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c66be09-866b-4b30-883f-c01d0068e9f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3401, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003401__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3402-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3402, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3403, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3724", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3403", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6163c3c-9385-4a8f-bbcd-1e46ba5cc440", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3402, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003402__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3403-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3403, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3404, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3725", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3404", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f5ff333-ea4f-44fa-8fa8-a829c74bbc6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3403, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003403__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3404-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3404, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3405, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3726", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3405", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b47aed02-073a-4f14-a004-7fe08bfdebe0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3404, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003404__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3405-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3405, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3406, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3727", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3406", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8cc84719-1c64-409f-9c2f-4264681427f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3405, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003405__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3406-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3406, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3407, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3728", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3407", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5232f7e-b4b1-473d-9784-c77b85225b1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3406, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003406__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3407-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3407, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3408, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3729", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3408", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d82b82a6-96f9-467f-9ef1-d35f164fd618", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3407, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003407__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3408-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3408, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3409, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3730", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3409", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec025eb3-0463-493d-a136-e8b58dfb67d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 295908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3408, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003408__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3409-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3409, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3410, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3731", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3410", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20f7e697-e9a5-45ba-8b1d-b73209745c8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3409, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003409__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3410-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3410, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3411, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3732", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3411", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd991338-135f-4423-8d37-e97360760fc6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3410, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003410__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3411-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3411, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "295953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3412, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3733", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3412", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2651539-38d0-4f52-af6b-d8f4df1172ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3411, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003411__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3412-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3412, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3413, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3734", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3413", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f261b225-823f-4e15-8dba-a68e6150bba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3412, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003412__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3413-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3413, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3414, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3735", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3414", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6117be9-7535-47f1-8568-3a949a6d85f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3413, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003413__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3414-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3414, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3415, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3736", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3415", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d16b145f-f545-456e-b0bd-3f7eae2e4605", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3414, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003414__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3415-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3415, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3416, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3737", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3416", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94dd83b4-6e1c-465a-8ad7-fb6688b76eba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3415, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003415__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3416-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3416, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3417, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3738", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3417", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06afaab7-ab29-45a6-855f-7e18fd1becdd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3416, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003416__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3417-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3417, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3418, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3739", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3418", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12df4062-9905-45f3-b71b-aaaee4ecf550", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3417, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003417__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3418-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3418, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3419, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3740", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3419", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79658b7d-ef8c-4c91-b80d-c072f34325cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3418, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003418__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3419-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3419, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3420, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3741", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3420", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76d6085e-abbc-4d79-8f5d-87b67e9c4572", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3419, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003419__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3420-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3420, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3421, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3742", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3421", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75471e57-42ef-48e3-a915-0d5cc03b2c16", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 296955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3420, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003420__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3421-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3421, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3422, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3743", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3422", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80cfa725-e8cf-4ca0-a9fb-9881c56f7d74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3421, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003421__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3422-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3422, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "296906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3423, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3744", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3423", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da290d1e-29dc-4a15-a548-8ad81b2f5c45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3422, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003422__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3423-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3423, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3424, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3745", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3424", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2662878c-624d-4d7e-9eab-28e53f73b064", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3423, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003423__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3424-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3424, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3425, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3746", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3425", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5893b157-4da8-449c-985b-6843975acdbc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3424, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003424__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3425-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3425, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3426, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3747", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3426", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d45abe12-5231-4cb9-8614-27a885188ba3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3425, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003425__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3426-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3426, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3427, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3748", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3427", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b0d8489-ae2b-41c4-9448-fe12680ce892", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3426, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003426__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3427-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3427, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3428, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3749", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3428", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcab0e69-263b-48b5-8188-fa201437f4c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3427, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003427__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3428-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3428, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3429, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3750", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3429", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adf15a02-3ad4-44a9-b79b-940dc5c28f90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3428, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003428__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3429-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3429, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3430, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3751", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3430", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e753873-aa5e-4c42-b7c7-5196bb411204", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3429, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003429__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3430-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3430, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3431, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3752", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3431", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e634312-e9cf-4f4a-bf60-8d361d5a3e71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3430, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003430__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3431-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3431, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3432, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3753", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3432", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5bcbf7c-0fee-4133-83de-4d6ca34159b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 297908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3431, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003431__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3432-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3432, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3433, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3754", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3433", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2e8d9d2-b8d2-4ae0-96eb-57fc4089daff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3432, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003432__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3433-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3433, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3434, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3755", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3434", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cbd3da6b-8e03-45b8-8290-47b06eabff4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3433, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003433__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3434-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3434, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "297953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3435, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3756", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3435", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "914bf559-a6d1-43df-b046-868edc83a483", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3434, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003434__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3435-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3435, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3436, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3757", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3436", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "008ae2fe-6c76-432f-88a7-8b90c470af7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3435, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003435__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3436-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3436, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3437, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3758", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3437", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccb29313-dfea-477b-9a19-08f1926c33fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3436, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003436__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3437-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3437, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3438, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3759", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3438", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b8f305c-684b-4fa5-9398-d76ed531aaae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3437, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003437__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3438-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3438, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3439, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3760", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3439", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "485196e7-a8e4-4fd7-96ba-747a0d36f928", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3438, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003438__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3439-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3439, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3440, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3761", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3440", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f9ab725-d717-4348-bf06-18ee75134b37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3439, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003439__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3440-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3440, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3441, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3762", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3441", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8cf2544-e24f-4c99-953d-b575c943b64e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3440, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003440__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3441-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3441, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3442, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3763", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3442", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "131cada1-57e5-422b-8a1b-dc69dc8b4689", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3441, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003441__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3442-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3442, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3443, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3764", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3443", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ddb24c7-b3fb-486a-9e89-a4e3287a5f7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3442, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003442__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3443-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3443, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3444, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3765", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3444", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7baff57-e4dd-4525-84ca-1cb502031f7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 298955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3443, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003443__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3444-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3444, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3445, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3766", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3445", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9420116-3598-4051-9a29-1c264b2e92b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3444, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003444__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3445-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3445, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3446, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3767", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3446", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55c0c980-1028-4f63-8804-73f90f10d3d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3445, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003445__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3446-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3446, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "298984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3447, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3768", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3447", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26775fe9-0922-4b42-950d-27bfb6e88a07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3446, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003446__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3447-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3447, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3448, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3769", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3448", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fb033c0-f557-4f78-9754-b0ecbe98d806", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3447, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003447__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3448-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3448, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3449, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3770", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3449", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31096ef6-d0e4-4351-8832-ffba9080d827", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3448, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003448__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3449-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3449, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3450, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3771", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3450", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "240c5d5c-ed69-4407-a2d2-0238bc2fe377", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3449, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003449__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3450-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3450, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3451, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3772", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3451", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02553f62-7260-4b78-b5f9-1d857dc3a4c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3450, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003450__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3451-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3451, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3452, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3773", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3452", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2916fed7-28ab-4d93-a25b-bfa4a11c8773", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3451, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003451__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3452-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3452, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3453, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3774", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3453", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0f58c96-8afc-40ad-9fc4-8c3f7e7cf0e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3452, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003452__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3453-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3453, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3454, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3775", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3454", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a19b5509-465d-410b-92b2-7df3d30beee4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3453, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003453__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3454-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3454, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3455, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3776", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3455", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6e98e5e-56c9-414d-bb4d-4e2aad1910ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3454, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003454__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3455-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3455, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3456, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3777", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3456", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd8758a3-e8e6-4e69-a4be-8758fc41fa38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 299986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3455, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003455__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3456-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3456, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3457, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3778", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3457", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0807294-591c-481d-be6f-f7c451178918", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3456, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003456__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3457-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3457, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "299937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3458, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3779", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3458", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "094acb8a-f7d8-4c05-9361-0222ae690f26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3457, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003457__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3458-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3458, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3459, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3780", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3459", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eaaebed8-d7d1-4bc6-a07e-0308fbc02fd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3458, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003458__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3459-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3459, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3460, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3781", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3460", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97be6cd9-0de3-4060-9340-d8655e6542eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3459, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003459__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3460-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3460, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3461, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3782", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3461", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a71f1a70-4cd5-4208-aabb-a8124e17587b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3460, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003460__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3461-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3461, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3462, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3783", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3462", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c38a009-6a52-49df-940b-9d6d36a21462", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3461, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003461__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3462-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3462, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3463, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3784", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3463", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72e14934-5ead-46c3-befa-6f8cfd03b557", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3462, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003462__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3463-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3463, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3464, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3785", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3464", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "893e8dde-5030-4a70-93f1-9abe0ef0acb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3463, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003463__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3464-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3464, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3465, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3786", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3465", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f308dca-03eb-468b-9e02-7c06aa5490be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3464, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003464__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3465-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3465, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3466, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3787", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3466", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2cd8f9c3-119e-4d5c-a603-99fb21074da1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3465, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003465__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3466-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3466, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3467, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3788", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3467", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "581af717-b993-4f75-9cfc-1b60a53822f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 300940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3466, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003466__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3467-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3467, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3468, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3789", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3468", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7be14370-6035-402e-bc5c-d99b6cf2acf4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3467, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003467__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3468-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3468, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3469, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3790", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3469", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "238e8aa7-8d84-4e7b-9ffe-75c7d46b15ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3468, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003468__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3469-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3469, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "300984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3470, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3791", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3470", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8d6e276-37a0-4cac-b7ef-5ad2b59f74af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3469, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003469__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3470-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3470, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3471, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3792", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3471", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b47e3de9-b66a-46fa-be49-92af749003ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3470, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003470__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3471-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3471, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3472, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3793", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3472", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95fc6475-4b6e-4655-9966-cde4a333f846", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3471, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003471__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3472-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3472, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3473, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3794", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3473", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "299349ea-89f7-4373-8cec-302d6e5436cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3472, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003472__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3473-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3473, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3474, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3795", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3474", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63272bc3-da9d-480b-bd35-e933f783db06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3473, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003473__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3474-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3474, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3475, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3796", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3475", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "acdc46c4-5d66-4ab0-b4f4-98cf3627c979", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3474, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003474__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3475-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3475, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3476, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3797", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3476", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe436759-d39d-491f-8b91-17e641ef4ff1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3475, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003475__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3476-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3476, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3477, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3798", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3477", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "816d60c0-10d7-49da-8bd1-7eeb85eae204", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3476, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003476__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3477-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3477, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3478, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3799", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3478", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccc2c985-0026-46ae-96b7-a47bdcacb4c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3477, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003477__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3478-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3478, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3479, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3800", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3479", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1be93613-2146-4ae6-a7c1-6c2c602280db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 301986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3478, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003478__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3479-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3479, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3480, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3801", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3480", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c889fdfc-a530-4542-b083-8f3ee173785e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3479, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003479__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3480-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3480, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "301937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3481, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3802", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3481", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66673a9b-183e-44ce-8444-5aaddd4fc72b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3480, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003480__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3481-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3481, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3482, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3803", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3482", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "073e3e46-c82f-4489-9d0e-91211fb9cd01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3481, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003481__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3482-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3482, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3483, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3804", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3483", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "388e3fe7-d494-4419-8e4f-0c287f80f2c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3482, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003482__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3483-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3483, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3484, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3805", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3484", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a061f75-0e26-448d-a388-52da8c715d38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3483, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003483__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3484-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3484, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3485, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3806", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3485", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d169abcb-50ff-432a-902a-491d0ae5cf3d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3484, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003484__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3485-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3485, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3486, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3807", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3486", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2720a5d-878f-4f7b-a1c1-2e2c6149ed70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3485, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003485__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3486-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3486, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3487, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3808", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3487", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3c63abe-c025-408a-8802-fd625fec89d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3486, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003486__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3487-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3487, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3488, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3809", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3488", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03f79511-953b-4285-b31b-a4bb81702f36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3487, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003487__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3488-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3488, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3489, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3810", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3489", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb06aa28-9859-4512-a097-2bfe3b5a8f21", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3488, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003488__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3489-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3489, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3490, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3811", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3490", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e22198f3-51bb-47e7-8fc2-526da175f0a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 302940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3489, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003489__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3490-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3490, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3491, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3812", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3491", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7028c37e-8c5c-40c9-8651-d9415287df91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3490, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003490__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3491-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3491, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3492, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3813", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3492", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ddfe7da2-c513-47fb-820c-4f1e97d123aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3491, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003491__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3492-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3492, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "302984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3493, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3814", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3493", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d3b2464-6a14-4a00-a0d9-77a6e7e3dbd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3492, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003492__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3493-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3493, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3494, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3815", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3494", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e846b88-e634-4cc3-a126-9169b47c75d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3493, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003493__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3494-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3494, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3495, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3816", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3495", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44e186ec-30a3-42b5-9245-c9f8f4f720a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3494, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003494__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3495-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3495, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3496, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3817", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3496", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2cdf6d87-26ec-401c-a7e5-6eb9fa68e4cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3495, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003495__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3496-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3496, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3497, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3818", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3497", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25356fe5-514a-40b8-ab9d-56fd663a085e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3496, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003496__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3497-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3497, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3498, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3819", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3498", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "367f4f3e-f05b-41dc-8b3a-fecbe4d8059c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3497, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003497__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3498-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3498, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3499, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3820", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3499", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6267541f-c0fe-49b6-a50b-39321e7cf156", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3498, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003498__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3499-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3499, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3500, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3821", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3500", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5c4b60f-575c-4140-add1-10cbe92c65eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3499, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003499__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3500-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3500, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3501, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3822", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3501", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b0127d5-e4d2-45a6-800d-951ab2b6f0d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3500, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003500__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3501-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3501, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3502, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3823", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3502", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57320520-efb7-4bdf-a55e-c72a1cdb180c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 303986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3501, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003501__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3502-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3502, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3503, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3824", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3503", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a8864cd-bbef-4222-9831-97da0855dd94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3502, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003502__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3503-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3503, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "303937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3504, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3825", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3504", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55664c72-6bb3-4ace-8f05-8fb3da84af96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3503, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003503__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3504-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3504, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3505, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3826", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3505", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "acd38178-3aa4-4e07-98fa-8ba2e1080faf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3504, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003504__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3505-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3505, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3506, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3827", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3506", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a3e9edd-8416-463e-a6e1-046744427233", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3505, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003505__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3506-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3506, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3507, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3828", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3507", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3884d7b6-6afd-491a-acc1-cc84ddda437f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3506, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003506__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3507-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3507, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3508, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3829", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3508", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f144912d-9239-4928-9017-a2892e1ff7b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3507, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003507__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3508-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3508, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3509, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3830", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3509", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6176b20a-4cf5-4ecd-80e3-dac05a1bf0cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3508, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003508__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3509-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3509, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3510, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3831", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3510", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b14e0d6-592f-4d96-8a7d-066f7966262c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3509, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003509__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3510-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3510, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3511, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3832", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3511", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a2b78d6-03c5-4a14-8f71-bceb17511260", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3510, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003510__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3511-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3511, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3512, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3833", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3512", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e2e0c0b-e905-4688-88f4-b54d14912c67", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3511, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003511__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3512-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3512, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3513, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3834", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3513", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73f3e54c-da18-4e7f-8dfd-a2c5974b1d49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 304940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3512, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003512__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3513-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3513, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3514, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3835", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3514", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03597c7b-e5e4-43eb-b7a6-b61470efdfaa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3513, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003513__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3514-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3514, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3515, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3836", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3515", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81193ca7-794c-4ae6-a588-1389e232f8d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3514, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003514__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3515-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3515, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "304968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3516, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3837", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3516", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "914609eb-517a-43ba-bdb6-52df30744b87", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3515, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003515__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3516-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3516, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3517, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3838", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3517", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "506e379f-cc42-4009-8020-50b9752aa290", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3516, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003516__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3517-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3517, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3518, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3839", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3518", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea16b289-501c-4d69-bc1b-9c01f29bd232", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3517, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003517__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3518-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3518, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3519, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3840", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3519", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9a8417a-4c02-4d9a-ad3e-84c41e8da131", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3518, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003518__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3519-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3519, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3520, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3841", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3520", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f902e54a-164c-4277-b1dc-50ec43b66604", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3519, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003519__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3520-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3520, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3521, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3842", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3521", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee411e6e-5945-441b-ad49-986151ddcb0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3520, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003520__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3521-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3521, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3522, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3843", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3522", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67387f1e-e338-4a1b-b12d-787f5d25f9f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3521, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003521__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3522-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3522, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3523, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3844", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3523", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "093e9820-cc68-4ce6-8da9-3c7876ee3a6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3522, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003522__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3523-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3523, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3524, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3845", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3524", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f2a055b-d615-4208-b7a5-992c079707ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3523, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003523__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3524-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3524, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3525, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3846", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3525", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fba01e7c-692f-4682-ab5c-6f7d7fcfe0d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 305971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3524, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003524__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3525-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3525, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3526, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3847", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3526", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b3eb9e4-4594-409d-9aac-9c0d068defc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3525, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003525__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3526-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3526, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "305937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3527, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3848", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3527", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e52dd11-70f6-45c0-96ab-6ed4f91304f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3526, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003526__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3527-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3527, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3528, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3849", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3528", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7945c2e7-334e-4223-9ada-267afa8db608", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3527, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003527__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3528-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3528, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3529, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3850", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3529", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c30a454-90d7-45c3-8f44-e471524991d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3528, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003528__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3529-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3529, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3530, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3851", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3530", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2cd403ac-691f-482a-b787-e7fd20c87ca4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3529, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003529__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3530-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3530, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3531, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3852", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3531", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a449793-6ca1-4d30-b3a7-7453bddd2dac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3530, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003530__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3531-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3531, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3532, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3853", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3532", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1e93be5-a439-4cfa-9187-55e0c903f286", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3531, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003531__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3532-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3532, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3533, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3854", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3533", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "989b10ea-7999-4550-bb87-83819f50c5d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3532, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003532__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3533-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3533, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3534, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3855", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3534", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5ffd38b-fa68-4732-9c8b-15d1c2a2ec57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3533, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003533__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3534-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3534, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3535, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3856", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3535", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d070b85e-a87f-4c22-bc8d-2efc6448feb2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3534, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003534__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3535-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3535, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3536, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3857", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3536", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ad22825-c52d-4e57-95dc-8ea3677804d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 306924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3535, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003535__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3536-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3536, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3537, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3858", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3537", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fcb4859-514c-4ef7-903e-88aea0a96c20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3536, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003536__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3537-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3537, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3538, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3859", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3538", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a0cc02b-d159-4771-b743-af7093134775", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3537, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003537__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3538-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3538, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "306968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3539, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3860", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3539", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fc27fb3-8e82-4972-97f2-18e992c23a4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3538, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003538__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3539-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3539, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3540, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3861", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3540", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab6a8eb3-1a17-4086-bc58-2676c01930bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3539, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003539__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3540-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3540, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3541, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3862", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3541", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44f5cb1a-3e52-47d5-a204-c354595279fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3540, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003540__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3541-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3541, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3542, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3863", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3542", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "119e2023-f860-4a9f-b92c-cf34ecbe9af1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3541, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003541__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3542-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3542, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3543, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3864", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3543", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1dca668a-cfdf-4a60-8788-f42652d89e94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3542, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003542__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3543-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3543, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3544, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3865", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3544", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00ac892e-1495-434c-af5b-d5c3947c1548", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3543, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003543__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3544-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3544, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3545, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3866", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3545", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e4f249c-f933-495f-8c07-7e5adf01ce1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3544, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003544__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3545-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3545, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3546, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3867", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3546", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f2d2466-29d9-47b6-a3b4-ab7d1cdf2faf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3545, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003545__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3546-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3546, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3547, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3868", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3547", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbc3df28-e61b-4146-a34e-12584fb2a7f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3546, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003546__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3547-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3547, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3548, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3869", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3548", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2306c67-f75d-4b7e-9a4a-05f5a0ae01d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 307971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3547, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003547__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3548-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3548, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3549, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3870", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3549", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55204e73-c3f0-49f4-beb8-06be7928bf83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3548, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003548__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3549-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3549, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "307921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3550, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3871", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3550", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6251b01-c9a7-445c-a4ef-1ec88328e740", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3549, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003549__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3550-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3550, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3551, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3872", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3551", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77c5339f-8e87-4f1b-b022-e3add60f88d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3550, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003550__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3551-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3551, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3552, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3873", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3552", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25ef7b5b-d162-4d7b-b28a-55efd45dfe66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3551, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003551__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3552-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3552, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3553, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3874", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3553", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed3dae1d-4594-4871-bef9-41f19f247a8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3552, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003552__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3553-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3553, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3554, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3875", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3554", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8e3abfb-f4a7-499b-8371-3698b7c12e55", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3553, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003553__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3554-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3554, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3555, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3876", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3555", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d696c7e2-11aa-4652-a7d1-8a56e50a695d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3554, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003554__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3555-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3555, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3556, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3877", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3556", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcf589ff-0f52-4fa5-99f3-8a85f49282f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3555, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003555__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3556-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3556, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3557, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3878", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3557", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c31897ab-095b-493c-bf9a-03e5e80a6aa1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3556, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003556__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3557-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3557, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3558, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3879", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3558", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7aa42fa7-5d9d-4109-b416-43700fc6a462", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3557, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003557__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3558-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3558, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3559, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3880", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3559", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8cb703b-62dc-4cd4-8269-b4cb5bac68d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 308924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3558, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003558__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3559-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3559, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3560, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3881", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3560", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f96a7af-30df-4774-94da-2ad6853bb0c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3559, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003559__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3560-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3560, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3561, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3882", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3561", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccca72d4-f981-46ed-aa36-2a0c01841c41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3560, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003560__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3561-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3561, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "308968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3562, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3883", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3562", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "316f77a7-0930-4b47-99dd-b6eba2901f32", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3561, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003561__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3562-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3562, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3563, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3884", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3563", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31fcab5c-d419-494b-ad4c-a0498f2b6b8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3562, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003562__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3563-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3563, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3564, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3885", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3564", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6472c07-64b6-40e0-8499-f8f6bbb9a536", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3563, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003563__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3564-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3564, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3565, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3886", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3565", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "978611e8-d279-462b-942e-7b66cf089570", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3564, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003564__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3565-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3565, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3566, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3887", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3566", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "755e8647-33e8-4e1a-be7d-45bc5b47237b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3565, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003565__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3566-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3566, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3567, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3888", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3567", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa707eb9-7a1d-449a-9769-74052cfd49c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3566, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003566__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3567-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3567, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3568, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3889", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3568", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28b0c683-1cd1-4a39-89c1-96ca28c43769", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3567, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003567__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3568-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3568, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3569, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3890", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3569", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7958e9c4-24bf-4473-8b4a-b7afd0cf0739", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3568, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003568__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3569-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3569, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3570, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3891", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3570", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ff264f2-7259-49d9-b090-15e1550c8afc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3569, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003569__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3570-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3570, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3571, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3892", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3571", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e5b69ca-0fee-4e7a-9591-265f50713d01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 309971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3570, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003570__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3571-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3571, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3572, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3893", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3572", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ae79718-8f7d-4471-b02a-568db4fc150a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3571, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003571__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3572-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3572, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "309921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3573, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3894", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3573", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7f8cfac-c93f-4cee-b9e7-7be8c4a647a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3572, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003572__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3573-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3573, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3574, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3895", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3574", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bcb7904-2afa-4164-ab70-3119f1b23e06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3573, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003573__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3574-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3574, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3575, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3896", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3575", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15a1c2fd-dcf6-4fa2-8036-c0d79186deb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3574, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003574__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3575-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3575, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3576, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3897", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3576", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe11a03a-6098-4d40-9485-257ad85fa4da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3575, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003575__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3576-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3576, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3577, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3898", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3577", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7fcdb75-8503-4086-a7ac-671fb1f5406d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3576, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003576__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3577-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3577, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3578, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3899", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3578", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f21ccef-7c46-4501-9e4c-a46f9f81e742", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3577, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003577__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3578-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3578, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3579, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3900", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3579", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "599ea8f9-639d-4665-82fd-7daf9111f20e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3578, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003578__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3579-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3579, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3580, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3901", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3580", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "332441cc-a364-44d9-8ed5-c1800fac435d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3579, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003579__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3580-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3580, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3581, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3902", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3581", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ead5103f-b74c-4867-ab8d-a1c5fd909183", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3580, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003580__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3581-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3581, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3582, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3903", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3582", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0618e421-d5d9-44cf-a215-5f56b9d7bed8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 310924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3581, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003581__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3582-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3582, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3583, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3904", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3583", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6cc9cd2-13de-4a2d-aee4-6d2137afb2a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3582, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003582__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3583-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3583, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3584, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3905", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3584", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e153d30-6aa5-42d0-97ff-356c85f45a99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3583, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003583__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3584-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3584, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "310953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3585, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3906", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3585", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "931d5bad-f6c6-4082-9253-489bfd1cd32b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3584, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003584__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3585-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3585, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3586, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3907", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3586", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9a16383-e024-4b68-9df8-079da46db03f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3585, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003585__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3586-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3586, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3587, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3908", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3587", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f90b69c4-154b-4821-b47c-9101453d6cee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3586, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003586__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3587-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3587, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3588, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3909", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3588", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bd378b9-44dd-4781-b671-47bb89de9763", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3587, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003587__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3588-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3588, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3589, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3910", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3589", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a32c59be-5a6f-4104-92dd-d0cdcf652afc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3588, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003588__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3589-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3589, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3590, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3911", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3590", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a13c6f05-ca2e-48e2-8bad-91404ae2ac62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3589, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003589__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3590-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3590, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3591, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3912", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3591", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "436762f9-ea05-4a4f-a0b6-c3a649796b4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3590, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003590__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3591-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3591, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3592, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3913", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3592", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "365f8d15-c2b7-4c27-8adc-92057f2f1471", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3591, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003591__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3592-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3592, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3593, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3914", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3593", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a107693a-f1ec-419d-9935-aa059544b262", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3592, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003592__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3593-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3593, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3594, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3915", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3594", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23a130f9-4196-4394-985d-4cf147769d7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 311955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3593, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003593__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3594-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3594, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3595, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3916", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3595", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c68d25e-ecd0-4eb3-af7d-7a34bf59a218", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3594, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003594__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3595-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3595, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "311921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3596, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3917", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3596", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6a228c7-3a1b-42c8-8ebc-08e0fedf6d22", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3595, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003595__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3596-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3596, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3597, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3918", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3597", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9daf9e38-d3d7-4fc9-9c94-2808b2e425f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3596, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003596__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3597-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3597, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3598, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3919", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3598", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63b519b4-71b7-480b-a522-2336635a2194", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3597, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003597__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3598-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3598, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3599, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3920", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3599", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6a322f1-54fa-4112-aed0-8652e12e9d48", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3598, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003598__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3599-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3599, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3600, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3921", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3600", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc0c6b5e-06be-4bc1-9d09-db2aa95c3346", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3599, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003599__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3600-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3600, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3601, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3922", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3601", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e504a21-6d40-44bb-8e11-ef771f69158a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3600, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003600__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3601-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3601, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3602, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3923", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3602", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45ec3c18-e6f9-41d6-a35d-d012c8c6a8b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3601, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003601__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3602-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3602, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3603, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3924", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3603", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebdd33d5-ada2-4008-af71-73bf9fb06835", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3602, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003602__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3603-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3603, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3604, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3925", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3604", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be2abbfe-c456-4b37-9ce4-1bcbb797e39f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3603, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003603__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3604-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3604, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3605, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3926", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3605", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2930c452-14e2-4ccc-b8b3-2aa65d287f57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 312924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3604, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003604__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3605-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3605, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3606, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3927", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3606", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2196f778-5a88-4eaf-a3da-043f86a791a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3605, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003605__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3606-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3606, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3607, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3928", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3607", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78cd36a6-bf51-422e-8ca9-d5cc805d2b81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3606, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003606__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3607-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3607, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "312953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3608, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3929", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3608", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "076fb4f4-4120-4818-81a6-c4ee3de28769", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3607, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003607__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3608-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3608, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3609, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3930", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3609", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56c531d3-2c5e-4d0f-a486-851798f4fa6f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3608, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003608__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3609-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3609, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3610, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3931", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3610", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca32daa0-6231-4a70-90c5-1fb7965c547a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3609, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003609__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3610-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3610, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3611, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3932", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3611", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "650fc091-d818-4d56-ac34-6faf6790bf70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3610, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003610__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3611-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3611, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3612, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3933", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3612", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "660dd2e8-3581-48dd-bdbb-f0c9c5e1a96a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3611, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003611__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3612-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3612, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3613, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3934", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3613", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e0faaa9-0fb1-408d-81e1-ffce28727dff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3612, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003612__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3613-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3613, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3614, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3935", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3614", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a96d2fb-04d2-488d-9f67-35230c02026b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3613, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003613__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3614-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3614, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3615, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3936", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3615", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a15489da-815e-4b27-bd40-a5e00727f6fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3614, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003614__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3615-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3615, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3616, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3937", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3616", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecb97d1a-ec16-4bc8-9da7-c6a0f917d82a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3615, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003615__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3616-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3616, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3617, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3938", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3617", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0baaaf8d-7499-42fc-8778-e3bebefc56a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 313955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3616, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003616__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3617-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3617, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3618, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3939", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3618", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "096beff0-ac73-4b9b-af20-9930b028d372", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3617, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003617__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3618-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3618, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "313906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3619, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3940", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3619", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65ffd5ea-dec1-463d-939b-1032d08be10b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3618, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003618__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3619-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3619, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3620, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3941", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3620", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "678b643d-d445-49bc-b7a6-bca2ec0a9582", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3619, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003619__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3620-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3620, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3621, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:23:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3942", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3621", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b1ab950-5ff6-4d46-9fea-a29b77cef7aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3620, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003620__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3621-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3621, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3622, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3943", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3622", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "556ce29e-e1ec-487f-bd8d-1f4ddba728b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3621, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003621__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3622-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3622, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3623, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3944", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3623", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94fc7943-7e18-4325-a7b7-0bdaafcfc840", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3622, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003622__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3623-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3623, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3624, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3945", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3624", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d628b621-81f2-404e-bb55-3c9de0e961d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3623, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003623__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3624-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3624, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3625, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3946", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3625", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "645b3dbf-7001-4c2b-b4b7-b929de54a29b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3624, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003624__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3625-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3625, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3626, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3947", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3626", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0a03968-7b5d-4295-8a63-03bfc32ee06c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3625, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003625__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3626-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3626, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3627, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3948", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3627", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcd5f6e5-760d-4cb9-83e5-1b715f905199", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3626, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003626__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3627-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3627, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3628, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3949", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3628", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0606823-0173-4d9a-87cb-e3730699626d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 314908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3627, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003627__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3628-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3628, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3629, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3950", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3629", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f88e3cc6-68e9-4b1a-84f0-504d9e70b4a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3628, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003628__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3629-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3629, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3630, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3951", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3630", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df30b618-02c5-4161-b066-9fb636f86078", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3629, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003629__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3630-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3630, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "314953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3631, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3952", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3631", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "193e352c-1a35-4c73-ab00-4772adfacea1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3630, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003630__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3631-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3631, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3632, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3953", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3632", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4baaddf4-b76d-4aeb-92d4-387d0ff7365d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3631, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003631__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3632-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3632, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3633, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3954", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3633", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b4ff2c4-42bf-4ef7-b578-e902f4690412", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3632, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003632__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3633-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3633, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3634, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3955", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3634", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfb43281-330b-4257-9708-74bf35d1c66c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3633, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003633__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3634-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3634, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3635, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3956", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3635", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e328c1a8-9518-464c-95bf-26b38cf5c152", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3634, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003634__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3635-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3635, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3636, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3957", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3636", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f18928bd-52d5-40f2-adb7-413e5b3fa42e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3635, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003635__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3636-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3636, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3637, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3958", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3637", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d864eb30-f815-4223-b226-ac69eea31065", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3636, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003636__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3637-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3637, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3638, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3959", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3638", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa1c897b-10ae-4bc2-ae5d-0aa9a748e391", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3637, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003637__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3638-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3638, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3639, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3960", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3639", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e8aa04e-439a-4695-a37b-9f9992a31abf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3638, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003638__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3639-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3639, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3640, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3961", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3640", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d05085bf-346f-4e98-b6ad-999ec2e6e707", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 315955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3639, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003639__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3640-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3640, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3641, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3962", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3641", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "972ecf22-a460-4aec-939f-0dab50a9d9b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3640, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003640__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3641-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3641, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3642, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3963", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3642", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "305f052a-952e-4702-8ef2-7dcfdc12cdbe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3641, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003641__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3642-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3642, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "315984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3643, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3964", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3643", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1a25fb9-f526-48db-8964-43f203dd3a66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3642, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003642__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3643-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3643, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3644, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3965", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3644", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ca01e77-62f6-488d-ab7a-bd770b88de8c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3643, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003643__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3644-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3644, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3645, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3966", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3645", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e46d2cb-ee54-42a4-a556-26e98f00301d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3644, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003644__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3645-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3645, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3646, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3967", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3646", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3a92556-e377-4ae8-89f8-9a616695ea9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3645, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003645__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3646-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3646, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3647, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3968", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3647", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68fc5ab8-a2b7-4ccd-9989-1b3facf0183b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3646, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003646__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3647-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3647, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3648, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3969", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3648", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "011cea3a-5f1e-40a7-941f-98491e80fdd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3647, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003647__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3648-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3648, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3649, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3970", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3649", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fbe16fad-acf7-47c0-bd42-9af5276ab90a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3648, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003648__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3649-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3649, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3650, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3971", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3650", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ff06c80-6560-48de-a970-243abfb3324e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3649, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003649__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3650-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3650, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3651, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3972", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3651", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b713c05c-ad09-448d-b3f6-8d8b8bce67fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3650, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003650__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3651-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3651, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3652, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3973", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3652", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b95b97cb-86dd-4eb4-ba59-d0cc8d1692da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 316986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3651, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003651__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3652-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3652, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3653, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3974", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3653", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "044688f0-d637-4eb3-953c-108a35722c84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3652, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003652__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3653-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3653, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "316953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3654, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3975", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3654", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "247ccaa7-66aa-46cb-a425-5382ce49b441", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3653, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003653__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3654-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3654, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3655, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3976", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3655", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61e1fc42-8015-4a73-a768-55c20d546ccd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3654, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003654__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3655-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3655, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3656, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3977", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3656", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3672d08d-258e-49a0-8a41-bc5b00666136", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3655, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003655__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3656-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3656, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3657, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3978", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3657", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "beeaa93b-4258-4ea6-9f31-81a0addbebd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3656, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003656__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3657-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3657, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3658, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3979", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3658", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10e0d9bb-3fe8-4ba8-a20b-a70e95f7dda6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3657, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003657__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3658-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3658, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3659, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3980", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3659", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9c86de0-a578-4783-8a60-caed1cda4972", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3658, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003658__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3659-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3659, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3660, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3981", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3660", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a99e2941-0f3e-4299-95e2-ae9296962fe0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3659, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003659__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3660-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3660, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3661, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3982", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3661", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fcebc770-888c-4a48-8f13-af60b9543c2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3660, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003660__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3661-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3661, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3662, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3983", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3662", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b89f1fb2-e317-4344-b9b5-eab5006f0e72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3661, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003661__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3662-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3662, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3663, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3984", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3663", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c57448cf-3629-4e31-a81a-c6a916af7cc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 317940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3662, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003662__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3663-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3663, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3664, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3985", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3664", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "804cbba1-e6d6-4134-9aab-f6ef46a056ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3663, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003663__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3664-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3664, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3665, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3986", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3665", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a1221c6-8f26-4bfb-bde2-5c7127b0d853", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3664, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003664__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3665-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3665, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "317984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3666, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3987", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3666", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4f19bcf-2ab3-4f29-b96f-624b66517f85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3665, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003665__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3666-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3666, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3667, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3988", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3667", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f69aacf-19dc-4627-bf6d-7bad5df8338d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3666, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003666__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3667-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3667, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3668, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3989", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3668", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9804210-d916-4b6e-8a81-c221a970a142", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3667, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003667__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3668-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3668, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3669, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3990", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3669", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fd074cb-8243-46c1-a452-a1c4200356cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3668, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003668__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3669-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3669, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3670, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3991", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3670", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51462dd3-5ef0-4e0d-be7e-51f2df278479", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3669, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003669__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3670-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3670, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3671, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3992", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3671", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b03892b3-dcb0-4c2b-aefa-7e78cf83f745", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3670, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003670__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3671-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3671, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3672, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3993", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3672", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "899248ae-cc2c-4651-b71a-f3fe69749bdf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3671, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003671__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3672-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3672, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3673, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3994", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3673", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56b4dd94-0b1f-48aa-8b6e-157eae496d26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3672, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003672__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3673-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3673, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3674, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3995", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3674", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "002ffe3d-f164-4814-81eb-c2df356353fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3673, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003673__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3674-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3674, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3675, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3996", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3675", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cd28b0c-6386-4277-a718-30c8531d9bbe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 318986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3674, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003674__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3675-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3675, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3676, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3997", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3676", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47cc7d21-082f-46f1-97b4-0b3c0731bc7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3675, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003675__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3676-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3676, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "318937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3677, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3998", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3677", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8953a12-8c90-4ff1-b907-5bb78c5918de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3676, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003676__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3677-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3677, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3678, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "3999", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3678", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9658034f-230a-415a-9ed5-a8b044a08585", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3677, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003677__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3678-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3678, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3679, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4000", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3679", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99e8d787-100c-4197-be5e-53340c7743af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3678, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003678__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3679-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3679, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3680, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4001", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3680", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5f2b3fb-4ea7-4aca-a0d8-7f79f63cb2d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3679, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003679__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3680-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3680, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3681, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4002", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3681", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e184434a-91ce-4439-b7b9-47dd52a9578d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3680, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003680__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3681-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3681, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3682, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4003", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3682", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7bc03cf-581a-47e2-adb8-f1e25ae29834", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3681, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003681__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3682-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3682, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3683, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4004", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3683", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73d91be5-f931-4c97-8ca8-4d9940d64fcb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3682, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003682__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3683-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3683, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3684, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4005", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3684", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82c092f4-877c-4987-93c7-2870e33ca8a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3683, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003683__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3684-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3684, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3685, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4006", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3685", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55a56fbe-f623-4a1f-b950-5bda642c2e04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3684, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003684__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3685-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3685, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3686, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4007", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3686", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfe65daf-8b88-4630-a747-58d6206f51b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 319940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3685, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003685__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3686-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3686, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3687, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4008", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3687", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4824365-005f-41ed-aad4-13e58dd4a532", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3686, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003686__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3687-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3687, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3688, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4009", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3688", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ca7d241-1424-4141-be76-2102853c00bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3687, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003687__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3688-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3688, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "319984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3689, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4010", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3689", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaec0ba1-4224-4d24-b502-97a5f0593b2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3688, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003688__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3689-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3689, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3690, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4011", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3690", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7bec1265-6fa7-4ed2-a10a-d1138bc88d15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3689, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003689__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3690-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3690, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3691, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4012", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3691", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e89e39ab-43c5-4410-86c9-c55ad58e26d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3690, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003690__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3691-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3691, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3692, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4013", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3692", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5bf5e25-33ef-4c4a-ae87-c00f5179ab36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3691, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003691__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3692-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3692, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3693, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4014", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3693", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9f90124-bc8c-4d8d-81cc-6bacb21b7252", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3692, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003692__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3693-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3693, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3694, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4015", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3694", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f38720b4-7ab2-4484-bc5a-45d950911900", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3693, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003693__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3694-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3694, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3695, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4016", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3695", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cca9e29b-3a34-4095-a1e1-747c0982f6c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3694, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003694__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3695-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3695, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3696, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4017", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3696", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea7c6e69-539c-4965-a3e2-702f95cb5424", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3695, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003695__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3696-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3696, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3697, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4018", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3697", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1310c34-c314-43fc-8ef6-7d4d359e2015", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3696, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003696__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3697-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3697, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3698, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4019", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3698", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2619ebbf-b0cf-4fe9-a635-0750fbed5c5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 320986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3697, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003697__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3698-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3698, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3699, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4020", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3699", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5a01235-05ef-4a80-9de7-31abfb89c9ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3698, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003698__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3699-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3699, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "320937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3700, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4021", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3700", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e513da42-2efb-4b96-9373-d77c6e5f251e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3699, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003699__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3700-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3700, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3701, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4022", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3701", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6ca6842-1c13-4c67-b477-0248b327963e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3700, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003700__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3701-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3701, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3702, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4023", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3702", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f0b8ef0-c5c8-4558-ae69-2cfd96e56073", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3701, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003701__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3702-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3702, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3703, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4024", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3703", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "658e5f5f-0d45-4cbd-9a42-a7d15c0f6d09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3702, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003702__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3703-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3703, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3704, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4025", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3704", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c9c42f2a-eaa1-452b-a942-bb26ade28c80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3703, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003703__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3704-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3704, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3705, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4026", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3705", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a836d96a-a176-4d9a-9c99-524e22296982", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3704, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003704__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3705-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3705, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3706, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4027", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3706", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "377d5631-5ccc-4e47-8e36-824882817116", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3705, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003705__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3706-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3706, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3707, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4028", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3707", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70a2bb59-0c21-4942-a072-aefd24b8d25b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3706, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003706__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3707-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3707, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3708, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4029", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3708", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a92ec91-52fa-4bef-bfb9-5b91b7b8b8fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3707, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003707__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3708-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3708, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3709, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4030", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3709", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff9ef3a5-2193-40f7-a0d2-a145556f4be9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 321940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3708, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003708__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3709-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3709, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3710, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4031", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3710", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cb68e60-9597-419b-bcd3-0159c4fecbb2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3709, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003709__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3710-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3710, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3711, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4032", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3711", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e02a3ba-d267-4593-90c3-f3b916035579", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3710, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003710__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3711-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3711, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "321968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3712, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4033", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3712", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8fa03f8-5e44-4313-82b1-ba9fb612cf59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3711, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003711__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3712-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3712, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3713, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4034", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3713", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d563a66-dd34-4451-a969-cbca55433772", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3712, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003712__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3713-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3713, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3714, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4035", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3714", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84a2c3ba-34d7-46ac-bf16-72398f8212e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3713, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003713__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3714-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3714, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3715, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4036", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3715", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "713f1258-9349-4e42-bd3d-fa16c8fbeed9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3714, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003714__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3715-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3715, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3716, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4037", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3716", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8bc9198a-7b10-4542-be24-8b16f4729216", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3715, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003715__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3716-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3716, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3717, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4038", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3717", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8d8fe2b-0b27-4d45-8510-a4343f197664", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3716, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003716__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3717-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3717, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3718, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4039", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3718", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd5873e7-00e3-4405-a0eb-b818f123f9a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3717, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003717__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3718-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3718, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3719, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4040", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3719", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "436d39b6-0942-41c8-a112-5513c652cbbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3718, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003718__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3719-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3719, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3720, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4041", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3720", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f610bb8c-61ba-48ba-9218-fbdc93bbb39c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3719, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003719__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3720-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3720, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3721, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4042", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3721", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d00bd521-caa9-4066-9959-63eb6a3e1237", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 322971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3720, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003720__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3721-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3721, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3722, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4043", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3722", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71887dc2-72e2-405c-a8ca-93b18d6f6a57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3721, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003721__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3722-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3722, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "322937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3723, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4044", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3723", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4adbbcad-862c-46af-8454-b32764ed5d0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3722, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003722__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3723-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3723, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3724, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4045", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3724", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66f05d01-44ae-4777-a9f8-307ca01b4478", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3723, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003723__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3724-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3724, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3725, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4046", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3725", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1442c91c-9112-4c10-a210-3271adf7705a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3724, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003724__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3725-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3725, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3726, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4047", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3726", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00e23642-1522-49f5-8eaa-293bd6d40bc6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3725, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003725__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3726-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3726, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3727, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4048", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3727", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36a5b049-3a18-44ae-bd49-39f3c82f0858", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3726, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003726__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3727-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3727, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3728, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4049", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3728", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa2b06b2-2f2c-493e-ad15-6c21c6beb97f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3727, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003727__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3728-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3728, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3729, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4050", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3729", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e6d1e00-6d29-4f29-ad44-0b40e1ea30e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3728, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003728__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3729-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3729, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3730, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4051", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3730", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28cf610a-b933-4d8a-9c16-276e89364b58", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3729, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003729__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3730-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3730, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3731, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4052", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3731", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "185ad62c-8e6d-4d19-a26b-5b5c45e656a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3730, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003730__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3731-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3731, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3732, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4053", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3732", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21e9657d-c460-4666-8993-b682c34d6ade", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 323940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3731, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003731__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3732-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3732, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3733, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4054", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3733", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e340f56d-f8d4-4302-9099-2975b4505435", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3732, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003732__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3733-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3733, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3734, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4055", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3734", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bac16fd-ba28-41e8-9c4a-319e5e44956d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3733, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003733__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3734-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3734, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "323968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3735, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4056", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3735", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff91e177-7607-48be-8478-6be93822c84a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3734, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003734__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3735-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3735, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3736, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4057", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3736", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baaa95fd-53e7-4749-934d-819c89b640ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3735, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003735__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3736-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3736, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3737, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4058", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3737", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25f046ad-0290-4335-b322-d9a915effb1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3736, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003736__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3737-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3737, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3738, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4059", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3738", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee0635a8-5b69-4def-b616-37d3b7296ece", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3737, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003737__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3738-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3738, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3739, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4060", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3739", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5782cf69-0075-40d6-8a49-d14448bd31ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3738, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003738__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3739-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3739, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3740, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4061", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3740", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bb00805-b162-4c8e-92d0-60bbd6fb09a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3739, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003739__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3740-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3740, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3741, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4062", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3741", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d39e3f5-57cc-41da-9f7e-f7954515d166", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3740, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003740__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3741-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3741, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3742, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4063", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3742", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20531992-93c6-4981-acf2-0e5e2e765fbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3741, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003741__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3742-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3742, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3743, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4064", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3743", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2cbbea0f-dee7-469f-b209-2a5085b43905", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3742, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003742__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3743-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3743, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3744, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4065", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3744", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9cb79ef8-379e-4a73-8b24-02734974f1b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 324971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3743, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003743__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3744-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3744, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3745, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4066", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3745", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b8e0e21-e22b-40b4-b41b-e53ca9b19c9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3744, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003744__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3745-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3745, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "324921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3746, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4067", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3746", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "281268db-07ed-47bf-9fd2-cdb4b5c1a88a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3745, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003745__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3746-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3746, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3747, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4068", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3747", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b9a7401-85ba-409b-bf98-d0e2136af239", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3746, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003746__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3747-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3747, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3748, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4069", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3748", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ef3ff93-67de-4e33-a2b4-606f1dcb53b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3747, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003747__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3748-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3748, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3749, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4070", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3749", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a0675ed-3359-459e-b6c9-3269e8ff540d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3748, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003748__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3749-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3749, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3750, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4071", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3750", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59b3c322-3ec5-4acd-9c13-faf2224e3106", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3749, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003749__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3750-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3750, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3751, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4072", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3751", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7fadb259-8095-4d84-b51d-43c41352f3b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3750, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003750__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3751-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3751, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3752, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4073", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3752", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "610f510d-d7c4-4bf0-a868-74b4b9a97cd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3751, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003751__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3752-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3752, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3753, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4074", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3753", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca93a657-1988-4d50-819c-0fa8f7f59b4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3752, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003752__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3753-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3753, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3754, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4075", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3754", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61d3ac0a-0adc-4694-aebc-5190184882e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3753, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003753__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3754-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3754, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3755, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4076", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3755", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f038394-4038-4895-a4b7-aabc0cbbfdfe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 325924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3754, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003754__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3755-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3755, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3756, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4077", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3756", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6b200d3-a0eb-497b-b5d5-57760f53e64d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3755, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003755__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3756-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3756, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3757, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4078", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3757", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e80dac1f-8578-43fc-b24b-536bd9b39eb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3756, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003756__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3757-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3757, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "325968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3758, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4079", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3758", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77a2952b-db25-4908-90b0-9e4510f68f9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3757, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003757__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3758-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3758, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3759, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4080", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3759", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b7907691-156a-4f03-a46c-994eeedea380", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3758, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003758__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3759-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3759, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3760, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4081", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3760", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba6ddabe-919c-4a22-9acb-ef2d29d0d1fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3759, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003759__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3760-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3760, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3761, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4082", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3761", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3b91d2b-9a51-445c-96a9-51c54137bb52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3760, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003760__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3761-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3761, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3762, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4083", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3762", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0dec9dd-1970-481b-a045-4b801999e41f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3761, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003761__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3762-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3762, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3763, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4084", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3763", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ba4d9a6-910b-4b6c-a3fe-9abc2044c085", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3762, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003762__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3763-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3763, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3764, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4085", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3764", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0609115a-6445-4719-b6f7-503f321850c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3763, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003763__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3764-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3764, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3765, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4086", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3765", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1255509b-ca07-4117-81b2-2704b18bd8cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3764, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003764__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3765-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3765, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3766, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4087", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3766", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cdb9e94-2246-4161-8981-6a2cba4ccec8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3765, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003765__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3766-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3766, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3767, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4088", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3767", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8061f4d-45d0-4232-bd3b-356fdcffc0ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 326971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3766, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003766__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3767-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3767, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3768, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4089", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3768", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "335e233f-5b00-4cef-98ea-c4aa22e59bb1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3767, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003767__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3768-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3768, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "326921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3769, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4090", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3769", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25f5c22a-b10a-49d6-84da-4bafe82c3895", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3768, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003768__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3769-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3769, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3770, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4091", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3770", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8713b1d-38ec-46ac-88b1-7d063e9edb83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3769, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003769__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3770-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3770, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3771, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4092", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3771", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e680f5a7-ad16-49a6-9226-dd7fad3547e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3770, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003770__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3771-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3771, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3772, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4093", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3772", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec9a055c-ba01-45dc-a914-16e3895db27d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3771, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003771__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3772-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3772, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3773, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4094", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3773", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68d6115f-2599-4e0e-af98-cc7108aea3cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3772, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003772__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3773-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3773, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3774, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4095", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3774", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38a3f019-c8a6-4162-a62c-e8c0f1630f2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3773, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003773__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3774-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3774, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3775, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4096", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3775", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc979521-1caf-4109-afd3-691b8283132c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3774, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003774__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3775-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3775, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3776, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4097", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3776", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58e04076-2208-4db3-8721-dcba4e1568ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3775, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003775__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3776-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3776, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3777, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4098", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3777", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad14b3e6-b7e1-4662-a693-2aadc8689fd0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3776, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003776__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3777-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3777, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3778, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4099", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3778", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad5e88ca-c6b8-463d-bdab-6f460e297d60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 327924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3777, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003777__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3778-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3778, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3779, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4100", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3779", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89362f69-ec48-4417-83ad-d505d1c4848f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3778, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003778__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3779-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3779, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3780, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4101", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3780", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1f804c6-f663-4efd-84cb-3152f838cba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3779, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003779__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3780-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3780, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "327968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3781, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4102", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3781", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63ed25ba-e0ec-40ea-9d2d-6bc4a366203f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3780, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003780__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3781-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3781, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3782, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4103", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3782", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f866a3c0-5f90-49e2-946d-a2a278df59c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3781, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003781__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3782-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3782, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3783, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4104", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3783", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "221e6851-6bd0-4875-851d-df51d2990a98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3782, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003782__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3783-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3783, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3784, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4105", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3784", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c9936adb-078a-4a2e-9971-4bb822bec871", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3783, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003783__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3784-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3784, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3785, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4106", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3785", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1228168c-dab7-4d86-83c8-db46a65ebe70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3784, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003784__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3785-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3785, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3786, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4107", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3786", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c73cf374-7335-42e4-87eb-65cc0f38605c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3785, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003785__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3786-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3786, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3787, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4108", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3787", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7fab23fe-e1b1-401f-a914-e5dfeee15b60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3786, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003786__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3787-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3787, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3788, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4109", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3788", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1621e7c3-db1f-413c-a478-5a39988b9e94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3787, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003787__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3788-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3788, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3789, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4110", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3789", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a2ac0c7-4fbb-4454-9e1f-3df66584c5b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3788, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003788__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3789-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3789, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3790, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4111", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3790", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f6118dd-6234-4fba-b9d9-bb64caa214c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 328955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3789, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003789__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3790-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3790, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3791, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4112", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3791", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b69a2cdc-a7cc-42d3-bc0a-59ff66d47a0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3790, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003790__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3791-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3791, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "328921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3792, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4113", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3792", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1905688e-6f8d-4e91-b8fe-5c1ce35495bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3791, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003791__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3792-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3792, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3793, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4114", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3793", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fc2cdad-7e80-4504-801e-b742278e5b46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3792, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003792__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3793-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3793, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3794, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4115", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3794", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48b552cd-32cb-41b7-87f1-e775958baba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3793, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003793__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3794-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3794, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3795, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4116", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3795", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7855f1e7-13d8-42ff-a638-f6151a4bbe24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3794, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003794__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3795-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3795, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3796, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4117", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3796", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b1dab3b-5135-4117-92f7-5c5d4e7f157e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3795, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003795__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3796-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3796, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3797, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4118", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3797", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "967a8bd0-cb86-4f3f-a65b-badf3d6670a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3796, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003796__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3797-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3797, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3798, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4119", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3798", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2fbd00a-f33a-4319-a882-5f03999ebfe0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3797, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003797__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3798-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3798, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3799, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4120", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3799", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65b30895-1421-4ceb-a679-b4e03eee39a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3798, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003798__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3799-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3799, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3800, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4121", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3800", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "572bc5d7-7870-4b35-9d30-ec418a73fbe7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3799, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003799__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3800-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3800, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3801, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4122", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3801", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5af53ce-69d8-494d-9ffb-7b5d2138e3d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 329924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3800, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003800__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3801-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3801, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3802, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4123", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3802", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69f9b011-4d6e-4759-8de5-a6120161aa9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3801, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003801__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3802-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3802, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3803, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4124", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3803", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6303e5c7-7124-446e-9dfe-68a3b77d834a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3802, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003802__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3803-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3803, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "329953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3804, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4125", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3804", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6fcb5de-6d1d-4a61-b3d8-5eeeea36c14d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3803, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003803__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3804-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3804, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3805, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4126", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3805", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9aa86ea3-2018-4e34-b8fd-c327d245e992", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3804, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003804__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3805-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3805, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3806, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4127", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3806", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf8a2654-5ee4-4d54-9f60-c26ff619eaac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3805, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003805__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3806-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3806, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3807, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4128", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3807", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5144683-7697-4622-b1f8-ccaf89498f50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3806, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003806__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3807-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3807, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3808, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4129", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3808", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c3c7021-5903-445a-8cc7-4e54b8bb421f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3807, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003807__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3808-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3808, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3809, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4130", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3809", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7dd50c69-bbd7-4ce0-b1e4-bfc193501205", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3808, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003808__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3809-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3809, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3810, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4131", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3810", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31d50d8b-df94-4653-a533-f539e64f4d87", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3809, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003809__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3810-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3810, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3811, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4132", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3811", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fcebd0f7-c83a-4d50-bf9e-6fa502e86518", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3810, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003810__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3811-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3811, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3812, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4133", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3812", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc47000e-dffd-4b92-a087-0908c5951067", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3811, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003811__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3812-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3812, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3813, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4134", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3813", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5ba10e0-a03c-4f41-93ed-f3e1500e9479", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 330955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3812, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003812__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3813-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3813, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3814, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4135", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3814", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa4c64f2-fe65-4c6a-8f1a-bb43067eda25", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3813, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003813__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3814-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3814, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "330906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3815, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4136", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3815", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97a1d351-86b4-4ec8-8cb1-45edfcd2592a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3814, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003814__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3815-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3815, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3816, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4137", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3816", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc0c6173-c168-401d-ad01-23fcaf8d2b53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3815, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003815__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3816-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3816, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3817, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4138", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3817", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34fa0c0f-4ffd-48bf-8be8-b85296339e95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3816, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003816__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3817-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3817, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3818, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4139", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3818", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8fb0e9e5-8d14-469f-9824-533c8bf26e23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3817, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003817__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3818-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3818, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3819, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4140", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3819", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10ca9664-a345-4d61-8939-4dc34765e37f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3818, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003818__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3819-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3819, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3820, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4141", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3820", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54a296e3-a835-45be-a637-7d8fd2611c62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3819, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003819__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3820-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3820, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3821, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4142", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3821", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7fae0cb-c0e7-42ea-b4ad-1f6ccf01db21", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3820, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003820__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3821-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3821, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3822, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4143", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3822", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abf87601-ef00-406f-9d26-7638e4620d08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3821, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003821__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3822-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3822, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3823, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4144", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3823", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "659d8a4d-4a4d-45ee-9418-f020309573f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3822, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003822__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3823-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3823, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3824, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4145", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3824", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab5ee38f-1c89-464f-8443-a7d821b8cfb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 331908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3823, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003823__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3824-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3824, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3825, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4146", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3825", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ce3f6ca-081f-4ba0-af2a-129426d08a5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3824, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003824__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3825-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3825, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3826, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4147", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3826", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1570263-54d3-494c-a417-10f2cc6e9385", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3825, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003825__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3826-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3826, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "331953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3827, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4148", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3827", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a0fb0a6-56ea-45dc-b60b-6dcce4e3634d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3826, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003826__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3827-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3827, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3828, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4149", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3828", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "477ff808-386f-4bea-879a-5f355d34fe4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3827, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003827__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3828-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3828, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3829, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4150", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3829", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1872f54-f3e6-4f1d-a953-e33c501b45b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3828, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003828__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3829-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3829, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3830, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4151", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3830", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "009f6063-e5bc-4d0f-8a22-2fd412be9ed6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3829, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003829__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3830-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3830, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3831, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4152", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3831", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a048b13-6499-4c19-b980-2b1168d41e8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3830, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003830__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3831-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3831, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3832, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4153", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3832", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5650d5d9-bb4d-46cc-8854-19df1e173610", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3831, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003831__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3832-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3832, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3833, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4154", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3833", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6216466-bbac-402d-99da-eb112df82328", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3832, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003832__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3833-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3833, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3834, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4155", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3834", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "588a7c17-4f26-4bb8-9345-bfd0bc5b71d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3833, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003833__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3834-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3834, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3835, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4156", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3835", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa9c3395-456a-4dee-afbf-070b86b0915f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3834, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003834__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3835-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3835, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3836, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4157", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3836", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "233f06b5-9ada-43db-a5fd-a8c21673b8ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 332955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3835, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003835__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3836-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3836, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3837, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4158", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3837", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70ec29c2-fb8a-49cb-b461-60d6e72d23fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3836, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003836__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3837-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3837, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3838, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4159", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3838", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff791b36-561d-401c-97e9-dbd3d43bd5f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3837, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003837__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3838-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3838, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "332984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3839, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4160", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3839", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fff93c2-7e27-424d-8ca8-0c498b23fc69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3838, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003838__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3839-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3839, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3840, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4161", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3840", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0034cfa6-46f4-4a8b-954c-c122976c3d24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3839, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003839__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3840-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3840, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3841, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4162", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3841", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9a27f05-fa04-4abc-82dc-4bc2722a135c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3840, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003840__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3841-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3841, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3842, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4163", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3842", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6111e93f-88c0-460d-a975-d5d8a0d8d54c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3841, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003841__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3842-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3842, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3843, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4164", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3843", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b6012b0-6c3b-4391-aaa5-54e831d7532d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3842, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003842__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3843-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3843, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3844, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4165", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3844", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff12b5a9-8ba8-486e-94dc-93a2d19e8b08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3843, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003843__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3844-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3844, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3845, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4166", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3845", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cc67e19-2923-4a2f-898b-a2648b0f0ad1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3844, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003844__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3845-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3845, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3846, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4167", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3846", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62855d05-0f76-4742-ae2d-faf642a04c8c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3845, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003845__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3846-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3846, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3847, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4168", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3847", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "785573f9-6a97-4689-9973-97909e7f4b33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3846, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003846__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3847-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3847, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3848, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4169", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3848", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2637f6a-0b33-4771-b22a-e841fce4eac8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 333986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3847, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003847__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3848-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3848, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3849, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4170", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3849", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f7c6cb9-36fe-456b-9329-ee5676a57909", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3848, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003848__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3849-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3849, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "333953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3850, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4171", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3850", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf80c47f-4aa8-4a4c-b24b-97f05315c0c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3849, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003849__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3850-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3850, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3851, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4172", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3851", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e44c59d9-f52a-4eec-808f-fe5bc96380be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3850, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003850__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3851-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3851, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3852, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4173", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3852", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d0722b2-0a4e-4bb3-bc89-0eb694dedcfa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3851, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003851__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3852-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3852, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3853, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4174", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3853", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5593d96d-31ca-42de-a101-dd145720e8bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3852, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003852__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3853-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3853, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3854, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4175", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3854", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71f5cfd1-dc60-4217-b35b-6b3096a7e57c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3853, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003853__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3854-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3854, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3855, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4176", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3855", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60bfda0a-1366-4f8d-b6c6-70635e5424ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3854, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003854__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3855-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3855, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3856, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4177", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3856", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "963ca9ff-e5d5-4a62-9435-8ccd4083e7de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3855, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003855__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3856-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3856, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3857, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4178", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3857", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91c6ce35-4fd5-4f92-9913-11a3a80037c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3856, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003856__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3857-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3857, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3858, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4179", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3858", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54387d2e-02bf-43ce-8795-24291223e6c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3857, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003857__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3858-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3858, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3859, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4180", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3859", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4183a8f-faa2-40e1-8e4f-57e4426f544a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 334955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3858, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003858__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3859-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3859, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3860, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4181", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3860", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e701d17c-2f03-46b9-b8ee-878bca9764a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3859, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003859__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3860-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3860, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3861, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4182", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3861", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92c0d923-23a4-44bc-a475-21a3606ea599", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3860, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003860__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3861-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3861, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "334984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3862, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4183", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3862", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "105f8521-55cc-4d37-b530-bfdc3f5d9808", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3861, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003861__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3862-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3862, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3863, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4184", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3863", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4dcac07e-f4eb-42e5-a005-7d84b93e403c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3862, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003862__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3863-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3863, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3864, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4185", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3864", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a860e2be-3bbe-4ec7-8884-3ab3997e145b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3863, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003863__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3864-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3864, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3865, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4186", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3865", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "823a21fe-c0b3-4bb6-88db-cae922dd08a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3864, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003864__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3865-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3865, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3866, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4187", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3866", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a24bc0b-11fc-4b05-9393-9c1971e7694a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3865, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003865__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3866-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3866, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3867, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4188", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3867", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "451ad5d2-a1a5-45e4-bd1d-167ac7f11fd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3866, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003866__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3867-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3867, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3868, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4189", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3868", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f4d13c6-d878-450d-aae3-3f18b1ed8b94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3867, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003867__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3868-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3868, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3869, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4190", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3869", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e509936-5b06-4e6a-a141-85700e6efcb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3868, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003868__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3869-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3869, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3870, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4191", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3870", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "487f51d7-fff4-4d09-bcd9-2c3d5d40fe9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3869, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003869__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3870-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3870, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3871, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4192", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3871", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41ec7e9a-cdb7-4a1b-85e5-3d6f6b93e4f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 335986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3870, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003870__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3871-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3871, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3872, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4193", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3872", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df52f3bc-3f04-43c7-9bb6-d5dfc5b13d6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3871, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003871__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3872-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3872, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "335937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3873, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4194", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3873", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38349614-7bbb-417e-be79-f519a8d2f326", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3872, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003872__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3873-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3873, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3874, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4195", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3874", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43d3748b-1acb-4d2c-a0ac-db71ea7f73ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3873, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003873__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3874-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3874, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3875, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4196", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3875", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5e5e410-c9ee-4131-91aa-43d7179979c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3874, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003874__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3875-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3875, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3876, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4197", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3876", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "215c55ff-fabf-41a3-b04a-9e8e9d096f89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3875, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003875__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3876-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3876, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3877, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4198", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3877", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "287c22d7-af63-4c2b-ac01-31ed1128cfe8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3876, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003876__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3877-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3877, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3878, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4199", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3878", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2267a249-17fa-494c-b0c9-814caa24350c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3877, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003877__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3878-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3878, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3879, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4200", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3879", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31e4e022-c275-4ead-b437-aaa9d4a1a885", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3878, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003878__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3879-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3879, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3880, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4201", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3880", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7af43808-c7ee-486a-9446-6243b7f0db78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3879, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003879__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3880-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3880, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3881, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4202", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3881", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "919d9e9c-6b6f-48e6-b042-b3d173765a57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3880, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003880__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3881-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3881, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3882, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4203", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3882", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f373548-ff69-4b52-b75b-2b4e23b0a383", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 336940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3881, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003881__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3882-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3882, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3883, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4204", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3883", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20124948-7acd-4a0f-97ab-391fa581991b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3882, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003882__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3883-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3883, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3884, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4205", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3884", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b6dea20-53a7-40ab-9314-a7882165050c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3883, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003883__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3884-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3884, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "336984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3885, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4206", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3885", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30ae1a3d-f43d-4945-9160-e7849d69a088", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3884, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003884__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3885-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3885, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3886, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4207", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3886", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "968f6907-cccf-4d32-a0b7-18a96752dcb4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3885, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003885__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3886-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3886, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3887, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4208", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3887", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b032e14f-2cf0-4721-9b7e-1997e976ce5f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3886, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003886__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3887-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3887, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3888, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4209", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3888", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d70fb379-a5ab-4d02-9399-18182472c2eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3887, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003887__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3888-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3888, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3889, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4210", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3889", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23338e3d-dc16-487d-9ed6-c47bdcea7f32", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3888, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003888__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3889-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3889, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3890, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4211", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3890", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9771050-d89d-43d7-ac6f-33ee3db9c91e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3889, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003889__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3890-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3890, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3891, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4212", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3891", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "036cf0e8-9a8b-4571-a7ab-86131d72d8b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3890, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003890__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3891-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3891, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3892, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4213", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3892", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6639cb67-5104-4db0-b399-d8f95945670b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3891, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003891__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3892-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3892, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3893, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4214", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3893", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "242b90b7-ad27-4acf-ac4e-c9748deb2db9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3892, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003892__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3893-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3893, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3894, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4215", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3894", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc3649dc-699e-40ba-810d-7ad4f0ef6d90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 337986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3893, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003893__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3894-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3894, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3895, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4216", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3895", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2154b3f-7699-4acd-a919-3dbe66e6e391", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3894, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003894__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3895-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3895, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "337937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3896, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4217", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3896", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "282c808e-12a4-4bfe-8691-78b1fdfbf5df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3895, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003895__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3896-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3896, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3897, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4218", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3897", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2484552a-db31-479e-bad2-9c3c8caf3d11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3896, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003896__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3897-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3897, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3898, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4219", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3898", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44f2c487-5c71-46b4-a641-208d1e3643cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3897, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003897__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3898-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3898, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3899, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4220", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3899", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "457ac47a-9ab9-4e25-aba8-ac678eaef9c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3898, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003898__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3899-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3899, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3900, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4221", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3900", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2fac448-a33b-4e3c-bcee-1924bcef5abb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3899, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003899__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3900-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3900, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3901, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4222", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3901", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4896909-683b-4adb-b38a-eb79ed0ac1c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3900, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003900__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3901-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3901, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3902, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4223", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3902", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e90c2ba0-865f-43cc-9b9c-b09bfa4f9dab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3901, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003901__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3902-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3902, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3903, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4224", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3903", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c459131-bd5f-40dc-aeca-5afe526f15b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3902, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003902__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3903-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3903, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3904, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4225", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3904", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81e9b1d4-668a-47c8-8818-6a2d37a9fce1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3903, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003903__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3904-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3904, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3905, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4226", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3905", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cfa3420-4271-403d-8cb0-c953bfe56c7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 338940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3904, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003904__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3905-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3905, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3906, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4227", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3906", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b30a8f8b-abdd-4f82-b550-d51db0a1b215", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3905, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003905__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3906-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3906, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3907, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4228", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3907", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d3f42b7-5e86-48f7-ac8d-d162a9ee5503", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3906, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003906__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3907-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3907, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "338984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3908, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4229", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3908", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0eb712b-0eb3-41f2-ad8e-7fa364a2a201", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3907, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003907__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3908-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3908, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3909, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4230", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3909", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f882acf-8346-4412-b7f2-90a8542b009f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3908, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003908__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3909-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3909, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3910, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4231", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3910", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c8158e6-0d6b-4518-ba2c-6c01c95bb58e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3909, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003909__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3910-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3910, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3911, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4232", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3911", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11919863-9e17-42d3-8f58-be5e79cc81f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3910, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003910__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3911-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3911, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3912, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4233", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3912", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4d7a79f-8c1f-4018-938c-13e71a29a48f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3911, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003911__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3912-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3912, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3913, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4234", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3913", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fbb2d1c3-8ee6-4963-924b-4af659749634", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3912, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003912__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3913-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3913, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3914, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4235", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3914", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "afcb1dc1-9caf-475f-9c19-7cc9e116f09a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3913, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003913__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3914-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3914, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3915, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4236", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3915", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb7ad55d-1576-4ac0-976b-32b0037426b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3914, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003914__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3915-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3915, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3916, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4237", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3916", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "819e6004-bd7a-4834-b86e-059f4e8f8192", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3915, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003915__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3916-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3916, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3917, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4238", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3917", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2317039d-ff28-4147-b790-63ed84c7bba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 339971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3916, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003916__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3917-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3917, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3918, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4239", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3918", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97518bc2-5715-424c-824f-a437de7e7560", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3917, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003917__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3918-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3918, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "339937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3919, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4240", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3919", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3938aace-705b-40a7-bcf7-e7d846f3a6cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3918, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003918__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3919-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3919, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3920, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4241", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3920", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b207365-1415-4f9a-b2a1-4c84396bf4f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3919, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003919__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3920-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3920, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3921, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4242", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3921", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4875d8ff-7144-4bb8-9ced-26464e06a8d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3920, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003920__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3921-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3921, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3922, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4243", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3922", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60ed46f6-5ddf-4cc1-97f5-4c5a43234636", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3921, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003921__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3922-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3922, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3923, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4244", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3923", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c5e8f93-c45a-49fc-8fde-20816ed9a12d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3922, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003922__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3923-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3923, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3924, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4245", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3924", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b73c64f-c2e9-450b-802f-f747bee5d7cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3923, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003923__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3924-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3924, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3925, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4246", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3925", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0f579d3-2770-48a7-b502-3317b151e16e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3924, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003924__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3925-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3925, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3926, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4247", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3926", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "afcb0489-9f25-4916-ba3d-3781ca383f0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3925, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003925__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3926-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3926, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3927, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4248", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3927", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c2a54bf-63a3-473d-a4d9-dd77b206c849", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3926, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003926__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3927-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3927, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3928, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4249", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3928", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d626576-6446-444e-b1b1-7fad9ec3a97d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 340940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3927, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003927__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3928-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3928, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3929, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4250", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3929", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "017a0338-fbac-4c20-8532-071da31bc25f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3928, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003928__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3929-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3929, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3930, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4251", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3930", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d84e05d3-fc5c-4e49-89a0-6ba6d38ae23b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3929, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003929__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3930-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3930, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "340968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3931, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4252", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3931", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b11dbe7e-1627-4357-919d-a4f112e7be03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3930, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003930__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3931-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3931, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3932, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4253", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3932", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83752b5a-efe5-4473-9bdc-0a1493f51eed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3931, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003931__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3932-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3932, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3933, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4254", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3933", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "642a4465-e9ea-4cd1-a58e-c9b6ee9cb6fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3932, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003932__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3933-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3933, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3934, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4255", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3934", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c45e990-2473-4af4-85a1-2ce89825554c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3933, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003933__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3934-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3934, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3935, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4256", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3935", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47be7bc2-9518-4146-8a62-399697ff09a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3934, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003934__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3935-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3935, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3936, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4257", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3936", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92794b6b-1497-4d36-97db-cd74732694d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3935, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003935__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3936-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3936, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3937, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4258", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3937", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7580db9-0ef3-4fa7-877a-c4f5b88e2a97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3936, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003936__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3937-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3937, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3938, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4259", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3938", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1af6a7f1-865e-4fc6-86cf-8861d1bc56a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3937, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003937__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3938-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3938, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3939, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4260", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3939", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a217d8bb-0165-4bd8-92c8-9cc240020cd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3938, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003938__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3939-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3939, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3940, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4261", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3940", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5ae83e0-701b-47d2-a070-d33c1cf1f2c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 341971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3939, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003939__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3940-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3940, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3941, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4262", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3941", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bedec09-282e-44d2-8440-33bb2fecf267", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3940, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003940__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3941-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3941, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "341921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3942, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4263", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3942", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ca46089-c6d7-4dd8-81d7-d58d20a00420", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3941, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003941__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3942-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3942, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3943, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4264", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3943", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00afd96d-8c34-41c8-9a70-8bbdec7eba81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3942, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003942__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3943-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3943, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3944, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4265", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3944", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "197039bd-ad96-42dd-8415-b8412b1e62d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3943, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003943__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3944-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3944, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3945, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4266", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3945", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f20b0b74-a4b0-404e-a6b4-26e03e88ba00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3944, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003944__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3945-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3945, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3946, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4267", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3946", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "110da261-e0e4-4f2e-9846-86e2286508a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3945, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003945__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3946-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3946, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3947, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4268", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3947", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e65ebfd7-8f28-4b41-853c-ac8fd9c4a934", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3946, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003946__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3947-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3947, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3948, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4269", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3948", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dee4aab9-fa53-45ec-a36b-cf73fe31e53a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3947, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003947__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3948-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3948, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3949, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4270", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3949", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d261c1b2-a27f-40a2-95f5-dd95625ca47e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3948, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003948__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3949-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3949, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3950, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4271", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3950", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6653f3b-7f6c-43cc-a137-1828ac0e72e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3949, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003949__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3950-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3950, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3951, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4272", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3951", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40512d92-c45f-47c4-b8a0-7627dfa97323", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 342924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3950, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003950__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3951-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3951, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3952, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4273", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3952", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65d4429a-e363-4a2e-a9d9-49f32088afa7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3951, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003951__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3952-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3952, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3953, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4274", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3953", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b95b3bcc-1a0b-4e21-8400-f2ec6c293d95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3952, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003952__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3953-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3953, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "342968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3954, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4275", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3954", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d94c6091-2065-4b8b-ad7c-642496ad0bf9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3953, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003953__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3954-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3954, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3955, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4276", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3955", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c6cc87e-ce31-4e9a-b1ac-c9b2679f062c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3954, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003954__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3955-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3955, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3956, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4277", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3956", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1df7fd4-75fd-457d-932e-791aea4664f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3955, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003955__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3956-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3956, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3957, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4278", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3957", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9820b406-28cf-476e-ae15-9c53fcaec0f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3956, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003956__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3957-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3957, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3958, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4279", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3958", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbc0d057-6fba-4633-863c-af9933959d99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3957, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003957__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3958-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3958, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3959, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4280", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3959", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e55c8263-f827-4a59-b018-8dfd20256dfe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3958, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003958__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3959-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3959, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3960, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4281", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3960", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fd1023f-f9b9-4c9a-8cc1-f8e060e27097", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3959, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003959__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3960-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3960, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3961, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4282", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3961", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c31ebcd-811a-4367-bb31-639a8159c0dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3960, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003960__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3961-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3961, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3962, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4283", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3962", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16d7247c-8da3-4078-9cb8-e1c872abbe2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3961, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003961__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3962-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3962, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3963, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4284", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3963", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd44a5f2-9226-42a4-abf0-447654416e8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 343971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3962, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003962__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3963-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3963, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3964, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4285", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3964", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66ff80f2-1e81-44ed-923c-77963815810e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3963, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003963__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3964-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3964, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "343921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3965, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4286", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3965", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7022dc7d-3bc6-40ef-aef9-318f3efe3754", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3964, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003964__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3965-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3965, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3966, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4287", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3966", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e7573cc-0ad7-40fb-9edb-df05995cd0f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3965, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003965__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3966-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3966, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3967, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4288", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3967", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d92574aa-0350-4e39-b6e5-67c016fed56a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3966, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003966__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3967-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3967, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3968, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4289", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3968", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4b9a45f-c099-4f10-b1c5-fa1b0f4d519b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3967, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003967__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3968-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3968, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3969, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4290", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3969", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c0abf18-c171-4f34-b51a-cddd466ecad2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3968, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003968__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3969-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3969, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3970, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4291", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3970", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed5e0802-11b6-44b9-95e2-f686ff0e5c7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3969, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003969__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3970-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3970, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3971, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4292", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3971", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80fd0823-0449-49a7-9661-9df8b71f5104", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3970, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003970__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3971-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3971, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3972, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4293", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3972", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5f6c32f-0f1f-4729-babc-e78bf6c7cac8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3971, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003971__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3972-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3972, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3973, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4294", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3973", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0d853dc-39f7-408a-bb5c-e67faf897f0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3972, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003972__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3973-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3973, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3974, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4295", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3974", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e122294d-9301-4a44-815e-c5bad9ac492c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 344924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3973, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003973__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3974-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3974, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3975, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4296", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3975", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50312e28-3bda-4dcd-b307-39a3027b5cbd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3974, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003974__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3975-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3975, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3976, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4297", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3976", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55525f26-6b71-446e-857d-292de1432f78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3975, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003975__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3976-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3976, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "344968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3977, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4298", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3977", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ce11c2c-b83d-4ee4-9bd6-8ad25d34402d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3976, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003976__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3977-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3977, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3978, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4299", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3978", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "374d77d5-983e-41fe-a766-a0c447514371", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3977, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003977__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3978-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3978, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3979, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4300", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3979", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af20b966-a725-4298-b69e-6b72ebad511d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3978, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003978__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3979-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3979, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3980, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4301", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3980", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13a1710c-3a8a-4db9-8f43-04be79180645", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3979, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003979__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3980-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3980, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3981, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4302", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3981", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3536d002-5120-40cc-bd76-f5c81a58a75b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3980, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003980__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3981-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3981, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3982, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4303", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3982", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72075a86-61ac-482e-95df-7bffa60e10d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3981, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003981__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3982-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3982, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3983, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4304", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3983", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efaefa85-cfe2-4056-ab2f-0afe5f45daec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3982, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003982__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3983-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3983, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3984, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4305", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3984", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb0855af-7bf4-4308-b83f-0c83c90ffabf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3983, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003983__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3984-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3984, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3985, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4306", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3985", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5ec256b-5bc9-4738-befd-0efc81cd6863", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3984, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003984__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3985-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3985, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3986, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4307", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3986", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81a949e4-fcd3-4b4a-bdcc-9a6c48f46585", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 345971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3985, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003985__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3986-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3986, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3987, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4308", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3987", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f0b2369-73ef-44c7-8e74-148458ff01ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3986, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003986__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3987-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3987, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "345921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3988, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4309", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3988", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f42c004b-9cbe-453a-830c-943c9b1ec8d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3987, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003987__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3988-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3988, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3989, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4310", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3989", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9aed84d-dde7-4fc2-a512-22f079e70b35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3988, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003988__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3989-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3989, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3990, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4311", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3990", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fd7f87a-ee56-4922-8192-13b386eb01ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3989, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003989__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3990-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3990, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3991, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4312", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3991", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28499dd0-57f5-49ba-9774-b617c015bf57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3990, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003990__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3991-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3991, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3992, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4313", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3992", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80690a0d-7c56-4d28-93c6-37df356d6323", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3991, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003991__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3992-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3992, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3993, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4314", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3993", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "843b0941-2120-468d-a18c-7538e52c6eda", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3992, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003992__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3993-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3993, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3994, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4315", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3994", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfe390c2-4c4c-450f-90b3-16546cdd8370", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3993, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003993__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3994-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3994, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3995, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4316", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3995", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea5504c8-0213-4763-a472-5a8201a2a81c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3994, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003994__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3995-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3995, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3996, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4317", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3996", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38347722-d888-43be-a31e-8273a13bb584", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3995, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003995__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3996-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3996, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3997, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4318", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3997", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15cfc7de-4268-473c-8fd4-0aa3bb929433", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 346924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3996, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003996__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3997-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3997, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3998, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4319", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3998", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f72f2378-25b9-4215-8cca-e0c68859a15b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3997, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003997__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3998-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3998, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 3999, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4320", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "3999", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63e8eb6e-b783-4ee1-bff6-1df3ed19b23e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3998, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003998__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-3999-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 3999, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "346953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4000, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4321", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4000", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84af2c44-3b1a-4e73-9867-f78cea180709", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 3999, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000003999__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4000-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4000, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4001, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4322", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4001", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "535b4b7a-a6f2-4485-8a52-b9afff7db9db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4000, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4001-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4001, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4002, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4323", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4002", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65c85e0e-c235-44fc-9ad7-842664b0bf9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4001, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004001__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4002-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4002, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4003, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4324", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4003", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da07123e-fd9b-4a3b-bf3c-fe48ae52a6c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4002, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004002__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4003-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4003, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4004, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4325", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4004", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baa6ea91-bd3e-440e-90e1-877675ede3c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4003, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004003__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4004-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4004, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4005, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4326", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4005", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54b78b79-63cb-4267-a70d-7f67cab7d553", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4004, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004004__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4005-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4005, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4006, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4327", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4006", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8b20586-ec7e-4bfd-9d65-86c74aa57390", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4005, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004005__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4006-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4006, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4007, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4328", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4007", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "081a81c5-4dc7-4e0f-b7a9-eff0160bf73c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4006, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004006__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4007-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4007, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4008, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4329", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4008", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50b613db-3ec8-4554-95ea-f643ccc7646d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4007, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004007__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4008-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4008, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4009, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4330", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4009", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "530aca36-9ba3-42a7-b9ff-f9dd3e396f36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 347955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4008, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004008__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4009-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4009, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4010, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4331", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4010", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9249aaf8-5149-414a-8faf-4b1e34f7c165", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4009, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004009__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4010-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4010, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "347906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4011, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4332", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4011", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04fcf430-163d-4438-bd2d-8291fa6c1035", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4010, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004010__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4011-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4011, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4012, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4333", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4012", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac5c7a30-3d33-40d4-93d0-8b82dabccc9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4011, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004011__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4012-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4012, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4013, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4334", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4013", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb3547ca-9a14-4d0c-a18b-090235276050", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4012, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004012__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4013-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4013, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4014, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4335", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4014", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9171fac0-0d7b-49ee-98f2-fdfed3b9d32a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4013, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004013__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4014-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4014, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4015, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4336", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4015", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ec73f40-907e-4640-8c73-cd8e5be58d4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4014, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004014__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4015-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4015, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4016, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4337", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4016", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae6c8f0d-9e25-4965-ae11-1a6dcc97d79f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4015, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004015__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4016-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4016, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4017, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4338", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4017", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af002c64-4257-42bc-820b-9da265439d7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4016, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004016__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4017-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4017, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4018, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4339", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4018", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efbfb6d4-cadb-4c8c-8d1a-763d7d726b70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4017, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004017__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4018-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4018, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4019, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4340", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4019", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2a62c60-2ea1-4222-9532-c5d4ed18a6f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4018, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004018__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4019-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4019, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4020, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4341", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4020", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b787702-0bd3-42d8-a168-e27eb5404048", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 348908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4019, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004019__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4020-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4020, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4021, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4342", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4021", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "510e553e-f77c-4965-95a7-9da89c150964", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4020, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004020__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4021-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4021, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4022, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4343", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4022", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1dc2adaa-bdb7-45c9-942f-4caf928e9fee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4021, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004021__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4022-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4022, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "348953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4023, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4344", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4023", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da2e7c1e-e3df-44fa-8d17-9d75d0312af5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4022, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004022__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4023-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4023, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4024, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4345", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4024", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f48c53e4-1dca-42c2-9258-57fa54a1cd48", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4023, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004023__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4024-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4024, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4025, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4346", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4025", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2a95adc-a420-4c1b-93b3-53f3f90cf113", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4024, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004024__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4025-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4025, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4026, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4347", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4026", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b13c0379-dc34-4d08-8546-63760c619a94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4025, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004025__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4026-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4026, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4027, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4348", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4027", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8753c483-4b17-422c-b611-dc76d3675966", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4026, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004026__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4027-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4027, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4028, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4349", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4028", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "435899ad-c06f-4513-a3b1-93309cf7a83f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4027, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004027__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4028-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4028, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4029, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4350", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4029", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d27e7212-e3c6-476d-90d3-d020a01367b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4028, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004028__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4029-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4029, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4030, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4351", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4030", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "49db5348-a9d8-41c3-b95c-14f0cec42b53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4029, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004029__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4030-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4030, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4031, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4352", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4031", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d729d4d4-55ba-4f34-bb17-7697e7c283c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4030, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004030__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4031-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4031, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4032, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4353", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4032", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ab3e0df-c661-4c4c-8dcc-8a756d6734ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 349955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4031, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004031__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4032-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4032, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4033, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4354", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4033", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b7f28c0-1078-42e3-9a21-0d74cb509dad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4032, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004032__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4033-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4033, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "349906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4034, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4355", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4034", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c950c6db-c160-42d4-8e0b-df453b33c25c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4033, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004033__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4034-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4034, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4035, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4356", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4035", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89ee17ff-33aa-4f1a-8919-97de3d09b07e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4034, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004034__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4035-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4035, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4036, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4357", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4036", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9545b42-dfc9-402d-8090-9ec7a7961e23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4035, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004035__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4036-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4036, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4037, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4358", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4037", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc2fbccb-29a1-470a-8701-68134bea1451", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4036, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004036__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4037-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4037, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4038, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4359", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4038", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b276a858-a644-418e-b901-51ec0b203b3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4037, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004037__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4038-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4038, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4039, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4360", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4039", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a19526e-dcda-43f1-90b9-d5c2fb56e411", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4038, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004038__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4039-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4039, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4040, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4361", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4040", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3a2a517-4324-4175-92c6-3fb50e8a2f36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4039, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004039__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4040-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4040, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4041, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4362", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4041", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26b3908f-7636-4c1a-9f65-4b0fab911299", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4040, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004040__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4041-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4041, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4042, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4363", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4042", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02fe38fc-8a8e-4054-b06e-240637f88cef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4041, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004041__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4042-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4042, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4043, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4364", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4043", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89c21406-b76d-42c2-9c9b-4f8f13a3de9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4042, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004042__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4043-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4043, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4044, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4365", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4044", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e319f7f-6c20-4ea9-962f-30f4728e3c9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 350986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4043, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004043__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4044-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4044, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4045, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4366", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4045", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae5fd5ea-29fc-48aa-902c-3875ade306ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4044, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004044__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4045-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4045, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "350953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4046, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4367", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4046", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "408f4c97-c9a3-4442-9b8b-ece54f4382fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4045, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004045__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4046-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4046, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4047, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4368", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4047", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02fb81a8-e34b-4ba2-865e-522689d90a65", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4046, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004046__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4047-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4047, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4048, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4369", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4048", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d79315b7-e70f-47c7-9716-c36a5489b661", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4047, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004047__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4048-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4048, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4049, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4370", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4049", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "141d04d7-cbc0-419e-8bbe-bbc00e2f9171", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4048, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004048__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4049-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4049, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4050, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4371", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4050", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e33e2308-1451-4901-b56e-eed1cef9f847", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4049, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004049__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4050-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4050, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4051, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4372", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4051", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99ad49ff-56db-44c5-b4cf-b4f3b741f95c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4050, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004050__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4051-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4051, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4052, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4373", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4052", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78b3ee5f-e72a-4e4b-86db-c1dca2829491", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4051, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004051__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4052-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4052, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4053, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4374", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4053", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5679533-b842-4163-a123-dba25aab6465", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4052, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004052__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4053-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4053, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4054, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4375", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4054", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a77d676-9163-4391-881b-b83b52e512a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4053, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004053__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4054-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4054, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4055, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4376", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4055", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e30fd930-4a40-4428-889d-eefb43d216f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 351955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4054, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004054__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4055-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4055, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4056, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4377", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4056", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "568e6bd7-5a69-4ca1-8332-19877abc1ae6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4055, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004055__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4056-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4056, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4057, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4378", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4057", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8d2abce-2c17-4b80-945d-89a1c204298b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4056, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004056__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4057-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4057, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "351984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4058, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4379", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4058", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1a98d4a-aec9-4ee6-90ce-807b45eeec1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4057, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004057__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4058-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4058, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4059, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4380", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4059", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "affa37c1-b4a8-4bfd-a2fb-2d55b7f22b97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4058, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004058__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4059-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4059, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4060, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4381", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4060", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39e8a91b-8205-4f14-9e5c-2b8d964edea7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4059, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004059__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4060-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4060, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4061, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4382", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4061", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "342decf4-0e9d-4423-b2e6-8bfa30922e0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4060, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004060__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4061-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4061, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4062, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4383", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4062", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7a288b7-654e-4073-bb15-50efb132df07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4061, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004061__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4062-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4062, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4063, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4384", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4063", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ae17429-645c-4f2f-8525-646d8fe88486", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4062, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004062__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4063-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4063, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4064, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4385", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4064", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4a149ff-01e2-401f-9a21-eba5e820bc53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4063, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004063__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4064-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4064, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4065, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4386", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4065", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b093b183-6b32-4042-a103-4c0e2168ac13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4064, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004064__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4065-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4065, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4066, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4387", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4066", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d74e650c-f189-4299-b8e5-b754fb67eaf3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4065, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004065__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4066-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4066, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4067, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4388", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4067", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a216c585-9bc3-4b26-aaac-f16331509e19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 352986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4066, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004066__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4067-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4067, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4068, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4389", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4068", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4cc15e7-3c28-4be0-8dc7-ef1de6e2bde3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4067, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004067__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4068-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4068, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "352937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4069, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4390", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4069", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4aa728d-f39f-410b-a583-dfa4afc85538", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4068, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004068__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4069-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4069, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4070, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4391", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4070", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "080b1734-3956-4cc3-ba9b-13b89d23170a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4069, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004069__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4070-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4070, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4071, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4392", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4071", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15f1e61e-2c2b-429d-ad4c-3fdb91680f31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4070, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004070__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4071-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4071, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4072, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4393", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4072", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "192122e2-01c7-40bc-8001-8df24afd10a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4071, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004071__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4072-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4072, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4073, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4394", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4073", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c875e7e-c9d1-47ee-9d4f-dceb1256ac63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4072, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004072__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4073-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4073, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4074, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4395", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4074", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28d9f498-85c1-4386-b91b-9ef3356442dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4073, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004073__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4074-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4074, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4075, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4396", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4075", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "155c4e32-439b-4872-b02c-7f72f85f6eff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4074, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004074__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4075-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4075, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4076, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4397", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4076", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd3228cf-96e3-42b6-a145-8d9cf7187809", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4075, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004075__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4076-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4076, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4077, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4398", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4077", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1f03d43-b2b4-4f52-845b-e63e41a2a7f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4076, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004076__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4077-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4077, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4078, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4399", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4078", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08662a7b-7bb9-40a1-89c1-585fd479075d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 353940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4077, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004077__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4078-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4078, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4079, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4400", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4079", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "053894d7-6aef-466a-8e7e-6fa068dfe165", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4078, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004078__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4079-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4079, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4080, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4401", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4080", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01b6336f-6556-4825-935f-923aec0d5382", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4079, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004079__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4080-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4080, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "353984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4081, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4402", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4081", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5552475d-c3f2-4d36-95c6-202a314c2ed7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4080, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004080__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4081-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4081, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4082, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4403", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4082", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ddd028c-3cbe-44da-a031-2a0804e27bd7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4081, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004081__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4082-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4082, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4083, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4404", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4083", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fbbc133f-213f-42c5-9dc5-ecbad6bf409f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4082, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004082__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4083-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4083, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4084, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4405", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4084", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "063a3eaf-86b9-45b2-b397-6a66edfa2031", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4083, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004083__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4084-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4084, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4085, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4406", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4085", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a450059-144a-4280-ac0b-340eec622d78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4084, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004084__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4085-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4085, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4086, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4407", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4086", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17699805-d239-46f7-8891-7d9e04c8bda3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4085, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004085__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4086-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4086, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4087, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4408", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4087", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aea41872-433a-443d-8400-ad7c8701c53a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4086, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004086__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4087-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4087, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4088, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4409", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4088", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9352cbf4-2fe8-4655-858e-2cc6a50a234f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4087, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004087__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4088-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4088, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4089, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4410", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4089", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23064959-712c-4639-92b3-88a0f27dcc68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4088, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004088__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4089-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4089, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4090, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4411", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4090", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db17961d-5c58-4454-8c38-1738bf4a8c7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 354986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4089, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004089__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4090-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4090, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4091, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4412", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4091", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f251b66-bf2b-49b2-97a3-4a8e4b68ca77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4090, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004090__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4091-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4091, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "354937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4092, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4413", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4092", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87f704cd-d357-4b0e-b7c7-f036e563a3a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4091, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004091__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4092-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4092, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4093, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4414", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4093", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eab97f3e-39a0-4a86-8b52-08cf2fe89f64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4092, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004092__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4093-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4093, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4094, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4415", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4094", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71853567-9ed9-4d8b-94a5-a24f78874b10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4093, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004093__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4094-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4094, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4095, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4416", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4095", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3a136e7-bf05-48c6-bff1-60d67f0c5452", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4094, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004094__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4095-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4095, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4096, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4417", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4096", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3d625e5-a58f-4377-8c75-17e8cdd3ac8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4095, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004095__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4096-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4096, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4097, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4418", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4097", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "798daf4a-5627-4d41-ad13-70ae14d5bef6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4096, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004096__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4097-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4097, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4098, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4419", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4098", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "affbc16b-3542-41ef-882a-effc90840f54", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4097, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004097__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4098-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4098, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4099, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4420", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4099", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31c0f677-3b32-419e-9cd0-5ee673df3278", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4098, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004098__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4099-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4099, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4100, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4421", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4100", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b090836-1bde-4273-8e70-d63fb410cdd0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4099, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004099__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4100-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4100, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4101, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4422", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4101", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37e93868-3dcf-4112-a274-bf3691cf6b2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 355940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4100, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004100__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4101-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4101, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4102, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4423", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4102", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82110e11-dd10-4c4a-af93-c4767c6f1ee2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4101, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004101__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4102-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4102, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4103, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4424", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4103", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8d0a1b9-e861-4053-aebe-5cd75ceb9cae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4102, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004102__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4103-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4103, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "355984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4104, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4425", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4104", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76ae286c-a58f-4d8c-9d14-2eae9d0c6cac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4103, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004103__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4104-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4104, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4105, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4426", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4105", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baba9df5-b032-4f83-b452-0b3b093610d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4104, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004104__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4105-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4105, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4106, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4427", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4106", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85ec2244-8979-4fef-88fd-2263f7a859fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4105, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004105__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4106-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4106, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4107, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4428", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4107", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b7e8d952-a8d8-4478-aaf2-7befdda9f737", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4106, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004106__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4107-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4107, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4108, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4429", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4108", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "847526a5-96a0-4d7c-8b67-b1d96ed825ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4107, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004107__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4108-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4108, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4109, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4430", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4109", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09ada576-0109-414d-a17b-42251b772742", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4108, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004108__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4109-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4109, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4110, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4431", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4110", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af1ef16a-89e9-411a-88b8-c0493228f61b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4109, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004109__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4110-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4110, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4111, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4432", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4111", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "461ed2ee-b47e-4b18-9511-ae0b624608bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4110, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004110__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4111-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4111, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4112, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4433", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4112", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60e36ec7-b2ae-4b91-9b3a-93a9ab82ae8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4111, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004111__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4112-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4112, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4113, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4434", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4113", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85887627-7531-4b7c-87f1-d23985b13e2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 356986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4112, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004112__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4113-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4113, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4114, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4435", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4114", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3f75907-2968-4dfc-8a2b-c70c7c8277ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4113, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004113__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4114-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4114, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "356937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4115, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4436", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4115", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7747680-58ab-4f06-91da-9e3a21fe6d5f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4114, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004114__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4115-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4115, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4116, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4437", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4116", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58bdb938-559f-45bc-ac3c-0bd70b59bd36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4115, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004115__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4116-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4116, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4117, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4438", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4117", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22471b5f-8ed0-4702-a520-ea69e14f88d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4116, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004116__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4117-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4117, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4118, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4439", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4118", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6fc0baa-76d3-4336-af31-eecd69e9faa8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4117, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004117__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4118-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4118, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4119, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4440", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4119", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9885349c-121d-4006-842b-391303c88a53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4118, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004118__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4119-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4119, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4120, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4441", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4120", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82f19205-ba3c-446e-92b0-97f2865efd81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4119, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004119__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4120-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4120, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4121, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4442", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4121", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb8afef4-ea3a-445e-b376-2a23efaeac69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4120, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004120__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4121-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4121, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4122, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4443", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4122", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a445878-bd97-4ed9-ac29-67ca38a50bea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4121, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004121__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4122-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4122, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4123, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4444", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4123", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4728a0a6-84a6-4d48-9eb5-30bc99823129", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4122, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004122__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4123-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4123, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4124, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4445", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4124", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a60cd8ab-5805-4814-9431-ca0a1df4bddb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 357940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4123, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004123__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4124-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4124, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4125, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4446", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4125", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94f39037-b123-4886-ae1a-a7e0c9430ece", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4124, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004124__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4125-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4125, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4126, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4447", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4126", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "775290f2-1dec-4412-9346-4ad8385bdb78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4125, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004125__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4126-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4126, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "357968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4127, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4448", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4127", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6d781e5-611e-48e2-af7f-e0baca078ec6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4126, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004126__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4127-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4127, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4128, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4449", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4128", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b40a992d-caf1-4e87-b31f-67f59f0bb369", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4127, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004127__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4128-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4128, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4129, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4450", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4129", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbc5e8a5-6e39-44fa-b6c5-f4edd4968075", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4128, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004128__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4129-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4129, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4130, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4451", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4130", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "635e62e5-b9e6-494c-9c59-8a86b367420c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4129, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004129__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4130-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4130, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4131, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4452", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4131", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "023907db-9ca0-43a5-8391-ac3944012eba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4130, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004130__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4131-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4131, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4132, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4453", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4132", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ea6fb76-fea1-453d-838b-4c2afa575596", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4131, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004131__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4132-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4132, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4133, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4454", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4133", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ae026f4-7984-4d10-8e88-54fabdb61ece", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4132, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004132__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4133-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4133, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4134, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4455", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4134", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdfa974a-5cb1-4440-86d2-f49ad2bf649b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4133, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004133__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4134-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4134, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4135, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4456", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4135", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98b6cfe8-3a7e-4e5e-99bb-117402d0d43a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4134, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004134__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4135-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4135, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4136, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4457", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4136", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0e2ee77-a4d2-419e-a0e5-0a97f88f8b76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 358971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4135, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004135__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4136-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4136, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4137, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4458", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4137", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccf32e9a-210d-497a-8473-a5998d6ee42d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4136, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004136__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4137-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4137, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "358921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4138, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4459", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4138", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "586f9afe-f6ce-42a7-973f-67819507ee0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4137, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004137__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4138-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4138, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4139, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4460", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4139", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09c769ab-bb9a-47b6-a3e6-9cdeffb2cbcf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4138, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004138__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4139-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4139, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4140, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4461", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4140", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "165bcb47-edbe-4b16-a116-de8c4ed2451b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4139, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004139__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4140-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4140, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4141, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4462", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4141", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6bc9d15-16d0-45c0-979b-44bd79e52b4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4140, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004140__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4141-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4141, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4142, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4463", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4142", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36c87fe6-12f3-4639-b8c0-05c6925fe17e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4141, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004141__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4142-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4142, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4143, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4464", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4143", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b02a23a4-2f33-4d4d-b973-ed745e8e0228", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4142, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004142__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4143-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4143, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4144, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4465", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4144", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18baca67-878b-4a83-8f67-ee33e6ce7c2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4143, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004143__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4144-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4144, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4145, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4466", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4145", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9c00a74-8fc7-48a1-9572-5148a65705e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4144, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004144__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4145-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4145, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4146, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4467", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4146", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5ecd6a4-694b-4d39-ae8c-44df03308c5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4145, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004145__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4146-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4146, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4147, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4468", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4147", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52e3e896-5795-41a9-b875-411811b053a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 359924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4146, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004146__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4147-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4147, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4148, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4469", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4148", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7ef352a-c6f1-4f7d-afdb-c7c2ba75f04b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4147, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004147__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4148-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4148, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4149, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4470", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4149", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01aaca7a-b567-4086-8960-27aa0cc5ddb0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4148, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004148__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4149-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4149, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "359968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4150, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4471", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4150", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "caac40a6-033c-4eb0-8660-8b3b379243f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4149, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004149__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4150-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4150, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4151, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4472", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4151", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58efa623-98ac-4b82-ae36-bc3ec1015a85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4150, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004150__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4151-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4151, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4152, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4473", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4152", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b9d5695-1cdb-455b-84ad-3e83d970960f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4151, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004151__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4152-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4152, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4153, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4474", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4153", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "377137f6-7795-45f6-8afe-fabd6b125878", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4152, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004152__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4153-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4153, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4154, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4475", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4154", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6dffc76-ab90-4f9a-a211-9f7a121ab4ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4153, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004153__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4154-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4154, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4155, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4476", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4155", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce65694b-4740-4e28-a843-96a6333db52e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4154, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004154__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4155-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4155, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4156, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4477", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4156", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ce06098-8120-4359-8e1f-a68f13fd0afb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4155, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004155__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4156-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4156, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4157, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4478", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4157", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8af1f8e1-dfe5-4708-a9a9-c29180a6a007", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4156, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004156__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4157-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4157, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4158, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4479", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4158", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad09f4f3-6204-4ea2-99b0-56b9892dec31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4157, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004157__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4158-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4158, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4159, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4480", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4159", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9761c8b-b7e9-40b3-8d8b-e84b9c5c7afc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 360971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4158, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004158__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4159-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4159, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4160, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4481", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4160", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84366604-9b95-423a-8886-6a196e4468bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4159, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004159__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4160-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4160, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "360921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4161, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4482", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4161", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf39d33e-fb60-4f7c-8ef6-60b51543ff28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4160, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004160__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4161-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4161, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4162, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4483", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4162", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea7950eb-983a-482d-8f33-807c3a76c40b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4161, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004161__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4162-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4162, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4163, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4484", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4163", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c777ea2-2630-4db1-8b58-3d7ee29cf2d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4162, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004162__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4163-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4163, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4164, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4485", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4164", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2397c73-a6c1-4e0a-b6fd-7bbe0dc91654", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4163, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004163__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4164-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4164, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4165, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4486", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4165", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2210ca9b-f71e-49fc-900b-65901b136be7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4164, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004164__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4165-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4165, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4166, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4487", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4166", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a72f720-de2c-4245-ae4f-8b23a3707055", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4165, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004165__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4166-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4166, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4167, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4488", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4167", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e6b6d28-719f-4481-99f8-7d4a91e0913e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4166, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004166__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4167-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4167, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4168, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4489", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4168", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c284225-6f75-498c-9928-54d064ddf182", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4167, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004167__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4168-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4168, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4169, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4490", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4169", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa581fad-c28b-4bfb-aeea-35ed789e251a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4168, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004168__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4169-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4169, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4170, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4491", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4170", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c38e406-6704-487b-af4c-85c94ce44f7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 361924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4169, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004169__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4170-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4170, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4171, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4492", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4171", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec23565c-b9a2-4cc8-8f90-6ed6dac5227b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4170, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004170__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4171-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4171, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4172, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4493", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4172", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47ded6a5-259c-4c2c-a6b7-350b408da976", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4171, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004171__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4172-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4172, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "361968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4173, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4494", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4173", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9f0ab68-775a-4121-aeac-387a467bcb0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4172, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004172__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4173-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4173, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4174, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4495", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4174", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9c21fee-5353-41e5-a770-e41e0a9b36fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4173, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004173__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4174-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4174, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4175, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4496", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4175", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e8ac43a-1efe-4621-90f2-350a50286c55", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4174, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004174__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4175-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4175, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4176, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4497", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4176", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "998c37d0-3f39-4dce-b1b2-38a3f3e103b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4175, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004175__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4176-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4176, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4177, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4498", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4177", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c3e21d6-8885-4daf-ae70-01e6bc2d80f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4176, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004176__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4177-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4177, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4178, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4499", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4178", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c9482c8-8248-4a7a-8f40-f66e70343578", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4177, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004177__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4178-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4178, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4179, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4500", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4179", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad4d5383-2ffa-4146-a6d3-f0b1a0ea9768", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4178, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004178__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4179-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4179, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4180, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4501", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4180", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "097e0160-0635-4f37-ae37-396fca9d019f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4179, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004179__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4180-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4180, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4181, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4502", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4181", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "137052c9-3bf4-4f00-84c9-8cccf3fb6efd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4180, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004180__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4181-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4181, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4182, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4503", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4182", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f48395fc-07f9-41d7-8196-29416b7de677", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4181, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004181__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4182-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4182, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4183, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4504", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4183", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "203e74db-caef-4d35-9643-3f41eec60689", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4182, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004182__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4183-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4183, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "362921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4184, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4505", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4184", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d50e431b-831d-4313-a51e-d1ba0a3dd524", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4183, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004183__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4184-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4184, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4185, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4506", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4185", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed395a3c-af55-49ff-b818-1d598567bcb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4184, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004184__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4185-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4185, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4186, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4507", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4186", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bfe19b2-6cdf-424f-a475-ad9e5afb17a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4185, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004185__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4186-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4186, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4187, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4508", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4187", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0c78079-55c3-40aa-9978-44f8de8b482f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4186, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004186__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4187-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4187, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4188, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4509", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4188", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01d4045a-68be-4e89-8b35-75c0e4495aac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4187, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004187__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4188-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4188, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4189, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4510", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4189", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67f66b3f-a5c4-4f7d-8b7f-e62b77685c79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4188, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004188__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4189-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4189, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4190, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4511", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4190", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cad9b82-f412-44ef-9272-c72ac9727ccd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4189, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004189__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4190-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4190, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4191, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4512", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4191", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56d985ea-01e4-4d67-846d-40a90e5dbff1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4190, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004190__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4191-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4191, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4192, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4513", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4192", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54007131-8c96-49fd-91bb-23e407359793", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4191, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004191__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4192-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4192, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4193, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4514", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4193", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "649d2ded-5d93-45ef-a709-5fbc16b68729", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 363924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4192, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004192__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4193-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4193, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4194, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4515", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4194", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27a3c5df-e59f-479f-9aa3-972048ec072e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4193, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004193__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4194-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4194, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4195, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4516", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4195", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "881edb1f-01a5-412b-8b5a-397072f6bbd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4194, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004194__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4195-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4195, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "363953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4196, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4517", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4196", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "448b6869-cad0-4ea5-a068-910589bb4bda", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4195, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004195__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4196-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4196, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4197, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4518", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4197", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "620048b4-c00f-4754-90f6-7953216250a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4196, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004196__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4197-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4197, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4198, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4519", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4198", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfe0be96-db40-4687-9398-35d8e0ef2b5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4197, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004197__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4198-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4198, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4199, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4520", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4199", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3b6a23f-3092-499d-a264-1eca2ef62577", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4198, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004198__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4199-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4199, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4200, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4521", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4200", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89d96dae-1dab-4074-a5cd-859c408c9a6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4199, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004199__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4200-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4200, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4201, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4522", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4201", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fd520d2-b291-433c-b82a-c67b1456e50b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4200, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004200__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4201-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4201, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4202, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4523", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4202", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed7f9c16-65f6-493d-93ed-6c622623b7c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4201, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004201__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4202-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4202, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4203, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4524", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4203", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "720c7125-cfb0-4856-9f44-1f46ec0f2177", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4202, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004202__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4203-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4203, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4204, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4525", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4204", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a562fb5-6fb8-4679-b48e-0e8e5a915edf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4203, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004203__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4204-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4204, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4205, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4526", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4205", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19d14159-d4e9-4dae-b5da-9396874b9eef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 364955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4204, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004204__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4205-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4205, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4206, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4527", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4206", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73f1922d-1164-4052-9b16-d89234601bb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4205, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004205__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4206-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4206, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "364906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4207, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4528", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4207", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b548d09a-a5fc-46bf-9192-bfa23461e6f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4206, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004206__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4207-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4207, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4208, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4529", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4208", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87125a85-7239-4e1d-b6c1-e2d9f6070b5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4207, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004207__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4208-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4208, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4209, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4530", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4209", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "730f9b60-a485-4d8b-9a9c-19e6186188f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4208, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004208__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4209-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4209, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4210, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4531", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4210", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c3eccd3-191f-477a-9382-c81115ef4ba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4209, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004209__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4210-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4210, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4211, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4532", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4211", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1122e3fd-657f-4a9e-9365-463b80eefe6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4210, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004210__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4211-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4211, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4212, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4533", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4212", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "080e672c-e5ad-48bb-b5ce-dc21d8b6fb82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4211, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004211__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4212-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4212, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4213, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4534", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4213", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5982e003-3d52-41c7-a6e4-9cd359df1577", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4212, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004212__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4213-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4213, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4214, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4535", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4214", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c3bb4df-aeb4-41ed-b5a4-c3a4d1411a03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4213, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004213__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4214-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4214, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4215, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4536", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4215", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "990541c9-7815-4416-bbf3-2d96978a036a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4214, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004214__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4215-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4215, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4216, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4537", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4216", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1022e999-ad8e-4dbf-983c-9b5c41f4ea2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 365908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4215, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004215__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4216-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4216, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4217, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4538", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4217", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5d562ec-8bfc-45cc-a252-541f12850f0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4216, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004216__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4217-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4217, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4218, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4539", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4218", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "434af1d3-5970-493f-ae07-e27097106611", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4217, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004217__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4218-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4218, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "365953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4219, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4540", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4219", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ec0f003-3c2d-4d30-b6f0-8938f8fecf6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4218, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004218__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4219-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4219, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4220, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4541", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4220", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc31dd35-25cc-479f-8446-cdc6371aa109", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4219, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004219__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4220-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4220, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4221, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4542", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4221", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72e45796-2b52-445b-a6c7-76e3482553b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4220, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004220__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4221-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4221, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4222, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4543", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4222", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e808007-8a95-45d2-8050-41c7d6928ee9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4221, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004221__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4222-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4222, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4223, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4544", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4223", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3af388d2-60e7-4511-8857-1ec92586c2ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4222, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004222__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4223-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4223, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4224, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4545", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4224", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f574c9d-4930-44fc-adda-86bdbebbd1c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4223, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004223__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4224-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4224, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4225, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4546", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4225", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "446c6cef-e68d-47ae-ae83-b32329a09d17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4224, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004224__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4225-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4225, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4226, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4547", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4226", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14a4d50d-5afb-4d8d-b0af-17af715e5479", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4225, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004225__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4226-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4226, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4227, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4548", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4227", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87ca94e2-5ba0-46c2-aee8-2823f45e05ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4226, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004226__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4227-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4227, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4228, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4549", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4228", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88d985c2-b085-4b89-8ac4-6f39e61ce39b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4227, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004227__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4228-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4228, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4229, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4550", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4229", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6124b43b-233b-4f58-8b03-56ff97f69438", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4228, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004228__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4229-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4229, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "366906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4230, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4551", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4230", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ccfb00a-3cf5-4126-8d73-bf0b1d8c87bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4229, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004229__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4230-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4230, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4231, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4552", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4231", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f9a2d9a-9981-4cfb-9a43-01441e6c10b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4230, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004230__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4231-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4231, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4232, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4553", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4232", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1368300e-0ec3-4d48-9a3f-06b8085831ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4231, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004231__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4232-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4232, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4233, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4554", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4233", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02ffb817-b493-4230-b4df-1d26dbb4e194", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4232, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004232__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4233-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4233, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4234, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4555", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4234", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e301cb8-055f-449c-9b95-62d4cbf0d753", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4233, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004233__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4234-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4234, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4235, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4556", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4235", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c479e4a-5db9-4f34-83d6-ac4236d8decb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4234, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004234__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4235-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4235, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4236, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4557", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4236", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ab79dcd-d648-48c2-bcf4-25b2dd8e5bd7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4235, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004235__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4236-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4236, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4237, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4558", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4237", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "387a361d-02b3-45e7-9762-1dd0d8b6b3be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4236, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004236__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4237-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4237, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4238, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4559", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4238", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a79bc3f0-e8f3-48ec-b25f-52641ceba843", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4237, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004237__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4238-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4238, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4239, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4560", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4239", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f14256c-cd7c-482c-bc95-1ee96a8d88e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 367908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4238, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004238__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4239-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4239, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4240, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4561", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4240", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4596268-83a6-46bf-8eed-157fe9a5ad6f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4239, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004239__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4240-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4240, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4241, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4562", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4241", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6e8ae05-f68a-4b4e-844b-7364fc7c5aff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4240, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004240__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4241-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4241, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "367953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4242, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4563", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4242", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fceaa5eb-b1ae-4f8c-b68f-690f5eb71327", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4241, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004241__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4242-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4242, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4243, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4564", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4243", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "507e0e21-c989-4174-b825-d73f789e7403", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4242, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004242__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4243-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4243, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4244, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4565", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4244", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62c9442a-e863-4389-a6c0-feada1daef89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4243, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004243__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4244-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4244, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4245, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4566", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4245", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2cda30f1-4392-41b7-9986-8985a469e7eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4244, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004244__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4245-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4245, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4246, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4567", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4246", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6120756f-d34c-4b5a-9aef-6a87a55293bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4245, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004245__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4246-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4246, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4247, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4568", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4247", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cba4e88d-8fc3-4e1e-8c9f-32f8aecf1a3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4246, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004246__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4247-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4247, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4248, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4569", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4248", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30dab605-aa51-412c-965a-f57452191d60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4247, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004247__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4248-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4248, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4249, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4570", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4249", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "968d27a0-4bb6-463c-aeff-af2a978724ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4248, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004248__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4249-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4249, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4250, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4571", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4250", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea8a793d-e18a-445b-a7ab-4c9b69ef04be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4249, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004249__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4250-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4250, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4251, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4572", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4251", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f444b058-aa99-4599-90c4-91d1206e0ff3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 368955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4250, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004250__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4251-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4251, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4252, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4573", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4252", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15f22211-052a-4b48-ab42-d2da4a4a045f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4251, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004251__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4252-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4252, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4253, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4574", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4253", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99eca43c-46d4-460f-b6f8-558744afcd8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4252, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004252__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4253-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4253, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "368984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4254, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4575", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4254", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6da3308-91f3-45fd-846c-b99561530f6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4253, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004253__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4254-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4254, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4255, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4576", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4255", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8118df45-1e7a-4656-8a2f-99d6e59e5372", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4254, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004254__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4255-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4255, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4256, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4577", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4256", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b544111-d4a0-4534-a6f1-d2c8c609c5cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4255, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004255__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4256-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4256, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4257, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4578", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4257", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1659e25f-c744-4855-a917-532576925a37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4256, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004256__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4257-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4257, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4258, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4579", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4258", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bab53491-295d-4bd0-9100-00d3da35e2c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4257, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004257__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4258-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4258, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4259, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4580", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4259", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9112f08-8d82-43ae-ae27-590cf24644b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4258, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004258__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4259-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4259, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4260, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4581", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4260", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e64a3626-2c50-4f67-8897-58935ce3db7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4259, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004259__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4260-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4260, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4261, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4582", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4261", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c03db120-64f3-4168-a631-3dabea4b3c37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4260, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004260__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4261-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4261, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4262, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4583", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4262", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b46ef2b4-c262-4f88-b3d2-0767b1f8ee29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4261, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004261__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4262-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4262, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4263, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4584", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4263", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6d1d637-8b7e-417b-98bc-e396e2a7a9b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 369986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4262, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004262__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4263-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4263, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4264, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4585", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4264", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6b9bb93-b1e5-4010-bd0b-2993f051bf93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4263, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004263__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4264-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4264, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "369937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4265, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4586", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4265", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0468e01-6b8d-4715-8a8c-756bcdb645cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4264, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004264__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4265-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4265, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4266, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4587", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4266", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51c261c3-0fa5-427d-8290-d3612f1be59b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4265, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004265__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4266-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4266, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4267, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4588", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4267", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "487b3818-e1f5-47ed-bf77-5fb0f77b0342", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4266, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004266__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4267-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4267, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4268, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4589", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4268", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db0da378-cc7d-4506-9a29-b85abd8bb26f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4267, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004267__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4268-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4268, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4269, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4590", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4269", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34e0063b-2c31-4427-8e90-0351f4b328c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4268, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004268__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4269-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4269, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4270, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4591", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4270", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "283249eb-507a-4a44-acc6-d628eeed9b2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4269, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004269__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4270-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4270, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4271, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4592", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4271", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c3796b3-39c6-4e91-b005-8e79767019df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4270, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004270__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4271-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4271, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4272, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4593", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4272", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f65a0528-ff94-4b8a-9b65-b9fe4353fc60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4271, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004271__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4272-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4272, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4273, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4594", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4273", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3057eea3-a22b-4541-8431-43855f7962fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4272, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004272__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4273-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4273, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4274, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4595", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4274", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a6cef0a-e854-451b-86eb-ee917b54b00a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 370940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4273, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004273__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4274-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4274, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4275, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4596", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4275", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b80ae377-ae97-43ae-9000-f187521147a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4274, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004274__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4275-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4275, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4276, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4597", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4276", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "589f32b2-b0a7-48c8-b7f5-0e093e143776", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4275, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004275__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4276-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4276, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "370984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4277, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4598", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4277", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adef2d93-b7e0-48e8-9a30-f01d3135bde2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4276, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004276__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4277-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4277, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4278, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4599", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4278", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df5f3759-5e0a-42db-b529-a83259d4ff3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4277, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004277__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4278-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4278, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4279, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4600", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4279", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d5cb016-e458-4f48-8955-0cbc1c916d81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4278, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004278__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4279-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4279, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4280, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4601", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4280", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21d46783-2747-4dc0-ba32-8d6fa487b0f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4279, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004279__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4280-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4280, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4281, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4602", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4281", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51d71133-e366-42ae-a9fe-491727392e64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4280, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004280__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4281-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4281, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4282, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4603", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4282", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13f2d9b7-75b4-439a-aa2d-088be3804cd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4281, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004281__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4282-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4282, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4283, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4604", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4283", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0ead8f4-f851-4935-a563-cd79582f6328", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4282, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004282__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4283-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4283, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4284, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4605", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4284", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46c40675-d47a-4b54-b6d0-714303a7f149", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4283, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004283__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4284-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4284, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4285, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4606", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4285", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "404793ba-85da-41ac-ad80-f092b6be9a9c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4284, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004284__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4285-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4285, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4286, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4607", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4286", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12d3f67d-c35a-42ca-a986-56ee398d5135", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 371986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4285, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004285__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4286-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4286, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4287, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4608", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4287", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4df306ee-aebc-4644-a105-205e4234e4aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4286, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004286__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4287-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4287, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "371937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4288, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4609", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4288", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a9f6b69-aea1-4c0d-b941-d95b0fe4877f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4287, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004287__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4288-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4288, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4289, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4610", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4289", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ed94476-bbc6-475f-8276-b38d100634f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4288, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004288__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4289-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4289, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4290, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4611", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4290", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "556ad069-1c5c-40c4-881e-39e6a9320af2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4289, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004289__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4290-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4290, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4291, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4612", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4291", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7adc07fe-b01c-4e50-99f8-1fcab32bc6bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4290, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004290__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4291-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4291, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4292, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4613", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4292", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed442b8f-97e3-4a28-a730-cc12252de62e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4291, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004291__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4292-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4292, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4293, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4614", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4293", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a813649-1653-4886-829e-d018b1f4345d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4292, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004292__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4293-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4293, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4294, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4615", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4294", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "488aaeee-f56d-4185-bcd6-dab23787eb26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4293, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004293__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4294-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4294, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4295, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4616", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4295", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73bbc4a2-d732-4795-8a9e-a6a2c8f7a3b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4294, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004294__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4295-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4295, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4296, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4617", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4296", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0520a0e1-8d2b-43b0-87c5-bae4b3f1fe07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4295, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004295__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4296-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4296, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4297, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4618", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4297", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "139863f3-6ac1-48bd-ac2b-f0baf56c0a97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 372940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4296, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004296__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4297-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4297, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4298, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4619", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4298", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6eb3afb-6650-4b92-bce3-25ecaebdef1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4297, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004297__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4298-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4298, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4299, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4620", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4299", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b699705e-103f-4091-a80a-2873f03b06d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4298, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004298__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4299-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4299, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "372984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4300, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4621", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4300", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c209cdd7-f7fd-44ec-901d-1aad55abf7cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4299, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004299__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4300-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4300, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4301, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4622", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4301", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2372b76e-8a93-4f5e-a758-e090577ff7ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4300, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004300__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4301-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4301, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4302, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4623", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4302", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8ec022c-73b4-479b-815a-fd8f550e504f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4301, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004301__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4302-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4302, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4303, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4624", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4303", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f29075dc-db58-4375-abaf-3e36acf43431", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4302, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004302__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4303-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4303, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4304, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4625", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4304", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d316001-d4b4-4a17-ac96-ec927f129ad9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4303, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004303__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4304-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4304, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4305, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4626", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4305", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da3136ed-a792-48c6-bde2-93d400282995", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4304, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004304__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4305-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4305, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4306, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4627", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4306", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a273794-5e33-43e7-bd1d-466cebe1e9ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4305, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004305__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4306-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4306, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4307, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4628", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4307", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3e44639-1f81-42df-b282-c6cf9ca7fa5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4306, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004306__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4307-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4307, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4308, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4629", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4308", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8438e99b-b465-4efd-a73b-c5112afddd97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4307, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004307__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4308-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4308, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4309, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4630", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4309", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06b2dcf6-f648-4eae-b379-8ecca1c527de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 373986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4308, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004308__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4309-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4309, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4310, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4631", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4310", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f35bf2a-44e2-4304-9c27-1805ef6979f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4309, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004309__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4310-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4310, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "373937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4311, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4632", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4311", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "407fb908-f0b5-4ee6-90ed-dcdf5aabfd14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4310, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004310__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4311-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4311, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4312, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:24:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4633", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4312", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82e46074-5290-4175-9573-1a124a3e57e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4311, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004311__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4312-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4312, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4313, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4634", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4313", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff451f17-d79a-4bce-8819-9fdc1fcf2adf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4312, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004312__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4313-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4313, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4314, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4635", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4314", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00195a06-da63-4431-9303-17b3a49ce45c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4313, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004313__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4314-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4314, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4315, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4636", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4315", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23b9f047-8a60-44fb-b56d-33013d85ba07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4314, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004314__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4315-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4315, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4316, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4637", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4316", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "617490f2-c043-415c-a1dd-160ddbf99af2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4315, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004315__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4316-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4316, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4317, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4638", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4317", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5b2eb02-076a-43d5-8bd0-77829ecbffba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4316, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004316__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4317-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4317, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4318, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4639", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4318", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c8b631a-ab6a-47a0-a66f-98953742b562", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4317, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004317__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4318-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4318, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4319, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4640", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4319", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e39ea3d8-21e3-46ff-b7aa-7808af07723a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4318, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004318__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4319-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4319, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4320, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4641", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4320", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fdee42c1-387b-4cd2-abd9-3540e79036e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 374940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4319, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004319__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4320-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4320, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4321, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4642", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4321", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ae95605-6ed1-4b98-9892-80238fadca5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4320, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004320__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4321-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4321, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4322, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4643", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4322", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baab13e1-8b81-4ba1-8270-e60281d21e74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4321, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004321__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4322-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4322, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "374968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4323, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4644", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4323", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45b35ecc-cff5-4819-b488-dce034c25eff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4322, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004322__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4323-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4323, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4324, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4645", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4324", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c692d54-fe1c-4ec8-adea-9d8a1c30ab73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4323, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004323__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4324-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4324, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4325, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4646", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4325", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7235022c-3cec-4af5-9e94-e599d6afbf47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4324, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004324__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4325-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4325, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4326, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4647", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4326", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b67bd2aa-ae36-4cc4-ab2c-d7f36696877b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4325, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004325__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4326-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4326, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4327, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4648", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4327", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89dbb527-1a01-4703-b88f-b8fb9cc78d8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4326, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004326__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4327-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4327, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4328, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4649", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4328", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f5a65e5-0026-4a6f-9feb-43ac327114fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4327, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004327__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4328-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4328, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4329, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4650", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4329", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db3ed951-e078-43da-969d-88330067bc73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4328, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004328__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4329-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4329, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4330, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4651", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4330", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "828e2860-1676-49bd-bda0-8f46e5f7d672", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4329, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004329__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4330-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4330, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4331, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4652", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4331", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0c867ee-66e7-4786-b4db-69973ee1bb1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4330, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004330__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4331-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4331, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4332, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4653", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4332", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d2f668b-de48-496f-8e06-da2b7abe6fd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 375971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4331, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004331__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4332-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4332, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4333, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4654", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4333", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "beb19ab7-da4c-4007-8d70-0ac582e501a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4332, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004332__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4333-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4333, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "375921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4334, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4655", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4334", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d79b77d2-97e4-497a-b9e9-fb539c4846c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4333, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004333__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4334-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4334, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4335, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4656", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4335", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bee496a-e4aa-49a7-915b-ff4c46368703", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4334, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004334__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4335-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4335, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4336, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4657", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4336", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6246162c-e310-40ff-aebf-9a1d538baf00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4335, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004335__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4336-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4336, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4337, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4658", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4337", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "269f48e6-3c91-4e05-b8d4-8c2b6ab27793", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4336, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004336__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4337-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4337, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4338, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4659", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4338", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "421b3dbc-acea-49e5-9824-e9b84433fca7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4337, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004337__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4338-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4338, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4339, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4660", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4339", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4f5cce2-4fbf-4457-9dc0-76fcc6e582d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4338, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004338__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4339-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4339, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4340, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4661", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4340", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ac0ab9b-28c3-4c65-afe3-410ca24b77a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4339, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004339__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4340-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4340, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4341, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4662", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4341", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0458e724-10cc-4c1d-bc6b-0d24e784256f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4340, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004340__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4341-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4341, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4342, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4663", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4342", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f414373-8add-47ab-b407-19a9aba3bf69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4341, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004341__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4342-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4342, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4343, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4664", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4343", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fce3dcde-eefa-49d6-9994-b5709a37bad7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 376924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4342, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004342__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4343-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4343, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4344, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4665", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4344", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d03b563-7c9a-4e3a-a422-c8f3c2233e50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4343, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004343__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4344-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4344, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4345, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4666", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4345", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de1c1c3a-3859-4f22-89a1-362c710d6a9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4344, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004344__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4345-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4345, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "376968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4346, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4667", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4346", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d975856d-4f9e-46aa-b58a-a6449f25e3b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4345, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004345__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4346-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4346, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4347, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4668", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4347", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ae2b221-f58d-445d-812f-c243db6556d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4346, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004346__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4347-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4347, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4348, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4669", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4348", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1430537-12b0-4bdb-a582-e61fdc274e8c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4347, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004347__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4348-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4348, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4349, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4670", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4349", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2c74090-71a7-4ed0-82b6-e05d40eda29b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4348, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004348__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4349-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4349, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4350, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4671", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4350", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a868c8ad-88df-494e-b1a1-61765edd390e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4349, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004349__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4350-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4350, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4351, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4672", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4351", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1d660ce-d2a6-4824-a2e6-4fe4859e2235", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4350, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004350__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4351-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4351, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4352, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4673", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4352", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcb0e7a7-8711-4572-9215-5a6a56026647", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4351, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004351__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4352-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4352, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4353, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4674", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4353", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cb9f8c8-f291-4e92-aaef-69d222cf70d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4352, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004352__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4353-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4353, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4354, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4675", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4354", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5216c3e-ffcf-42e8-862b-afce0985be36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4353, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004353__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4354-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4354, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4355, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4676", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4355", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cd2a4db-c8fb-4ab4-a01a-2e9b60df8e30", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 377971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4354, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004354__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4355-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4355, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4356, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4677", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4356", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "650c72ad-d324-436b-b6e8-a5a6f63fcbc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4355, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004355__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4356-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4356, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "377921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4357, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4678", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4357", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed5c17f6-3c90-4b29-89c8-4e6a99eccfc7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4356, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004356__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4357-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4357, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4358, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4679", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4358", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54e8dc34-08b3-48dd-96b5-80981669e6d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4357, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004357__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4358-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4358, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4359, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4680", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4359", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81aa7cea-03f1-4119-9499-642ead346320", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4358, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004358__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4359-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4359, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4360, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4681", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4360", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de703538-24f3-427d-951b-f3c919aa83a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4359, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004359__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4360-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4360, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4361, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4682", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4361", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "304b1f24-9921-46f5-81d5-4375cca78daa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4360, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004360__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4361-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4361, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4362, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4683", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4362", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78821b90-7484-44bc-824c-efbd9b250a2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4361, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004361__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4362-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4362, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4363, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4684", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4363", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a8543409-bf8c-4283-ad11-cb793e91646a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4362, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004362__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4363-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4363, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4364, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4685", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4364", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e39eb2e7-1e74-4a7b-8af8-282e1a8ecdcd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4363, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004363__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4364-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4364, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4365, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4686", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4365", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d98c9fb2-b58f-4130-a01b-57ebb1e3b0e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4364, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004364__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4365-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4365, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4366, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4687", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4366", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08440d2f-c37e-4e59-891c-f77d4a7e588a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 378924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4365, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004365__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4366-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4366, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4367, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4688", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4367", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1491a72e-a81c-4932-9813-80bcf070fed2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4366, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004366__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4367-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4367, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4368, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4689", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4368", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "113edf5e-1cc7-42e3-a005-dc93925ba938", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4367, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004367__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4368-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4368, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "378968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4369, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4690", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4369", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc9d5063-e7c3-488d-a3ec-7ef91fc7800a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4368, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004368__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4369-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4369, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4370, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4691", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4370", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe4af0c2-4bc3-43e5-9d2f-df7c61bf98de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4369, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004369__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4370-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4370, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4371, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4692", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4371", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f29e4f2-05ea-49a9-8a81-93a582d9b343", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4370, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004370__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4371-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4371, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4372, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4693", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4372", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "674f18e7-c113-4e86-8ac1-4442a547e0fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4371, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004371__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4372-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4372, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4373, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4694", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4373", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b1a1bb0-574e-4f8f-8f58-9b08b0ed8c0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4372, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004372__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4373-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4373, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4374, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4695", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4374", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b62edb29-c4da-4a44-bddb-b0b0f334b921", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4373, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004373__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4374-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4374, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4375, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4696", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4375", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "663ed843-c940-4100-8fc3-6d356a63b58a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4374, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004374__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4375-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4375, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4376, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4697", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4376", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a57405d3-8c49-45f5-9092-102b38abb06b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4375, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004375__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4376-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4376, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4377, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4698", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4377", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "238db2fa-0ca3-43e9-9178-703513046e7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4376, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004376__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4377-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4377, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4378, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4699", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4378", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d223413c-436b-434f-bd63-2406e30b7ab1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 379971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4377, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004377__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4378-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4378, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4379, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4700", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4379", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ebcf73c-7207-444e-baa3-067e5c4b9d0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4378, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004378__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4379-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4379, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "379921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4380, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4701", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4380", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb312cff-eb29-40c7-8ed2-e7f032f8582c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4379, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004379__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4380-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4380, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4381, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4702", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4381", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9878b7a-9383-48ca-b5bb-0e828de3e3be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4380, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004380__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4381-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4381, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4382, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4703", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4382", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ef8028b-365f-4206-8dcb-fa727cf739bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4381, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004381__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4382-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4382, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4383, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4704", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4383", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6698418e-e10a-442c-8edf-c358ae8a24c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4382, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004382__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4383-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4383, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4384, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4705", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4384", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fecb514f-bb75-4422-99e4-608a5c6e3f7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4383, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004383__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4384-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4384, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4385, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4706", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4385", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c15fd034-4c95-4d9b-82ef-e017bf6c1591", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4384, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004384__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4385-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4385, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4386, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4707", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4386", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9b162d1-c4bb-449e-9135-46262e11b02a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4385, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004385__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4386-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4386, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4387, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4708", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4387", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "718eae84-065f-4a6d-a1a5-ce7e173261ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4386, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004386__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4387-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4387, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4388, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4709", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4388", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d14b0eb-9569-4dc0-ade0-a756f1aa65bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4387, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004387__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4388-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4388, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4389, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4710", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4389", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb2ae9a5-1a39-467b-b2e8-57414bb7a9b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 380924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4388, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004388__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4389-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4389, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4390, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4711", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4390", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64bfaedc-2c51-4bff-a6cf-6b4533a508d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4389, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004389__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4390-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4390, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4391, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4712", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4391", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d03eebb6-723d-4034-ae65-fe65bedc3da6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4390, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004390__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4391-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4391, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "380953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4392, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4713", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4392", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20a53bd3-04b8-49cd-8fdd-dad113fc0bca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4391, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004391__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4392-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4392, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4393, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4714", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4393", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "174960b6-9cd9-4bc8-bbf2-277da968a197", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4392, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004392__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4393-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4393, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4394, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4715", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4394", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "49dc4e48-46e5-4c03-93de-fe46c76165fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4393, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004393__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4394-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4394, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4395, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4716", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4395", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00670f7e-fec6-4b63-8520-56869627ce59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4394, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004394__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4395-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4395, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4396, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4717", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4396", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "377d7bcd-686e-4ea7-a0a2-1980b7686ad7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4395, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004395__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4396-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4396, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4397, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4718", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4397", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac762aac-9052-478f-a390-af9dc5561d9c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4396, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004396__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4397-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4397, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4398, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4719", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4398", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b64c569a-3e26-4702-82e7-d6f0d90bad6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4397, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004397__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4398-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4398, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4399, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4720", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4399", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94942b44-2b77-459f-aa28-23f42937b607", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4398, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004398__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4399-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4399, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4400, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4721", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4400", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f5ca76e-e1ca-42cf-8337-ade0fea6d616", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4399, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004399__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4400-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4400, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4401, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4722", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4401", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62673f33-4c36-49c6-99b6-14936ff916d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 381955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4400, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004400__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4401-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4401, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4402, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4723", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4402", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68c00c99-8e2c-4994-8070-b522a69fe4ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4401, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004401__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4402-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4402, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "381906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4403, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4724", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4403", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09cff718-4581-4ddf-9cba-95cd6ac3f7e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4402, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004402__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4403-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4403, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4404, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4725", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4404", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6a84972-28f4-43c7-b106-42cb58369827", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4403, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004403__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4404-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4404, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4405, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4726", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4405", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74b6d6af-e7b7-4800-97bd-ee102f2c01c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4404, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004404__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4405-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4405, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4406, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4727", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4406", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b263f858-b3fe-4c0f-b6dc-7fcf70ce2366", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4405, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004405__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4406-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4406, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4407, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4728", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4407", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6383cb01-7680-44e7-97bc-d1e4f1956d4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4406, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004406__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4407-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4407, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4408, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4729", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4408", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8dd60250-6315-4432-a947-c9d20ced3a4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4407, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004407__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4408-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4408, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4409, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4730", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4409", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97854f46-edd3-4abd-99b1-d86154546f25", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4408, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004408__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4409-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4409, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4410, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4731", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4410", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b76da9f0-5ed5-4bc8-ac2e-c73fdc895808", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4409, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004409__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4410-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4410, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4411, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4732", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4411", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05c9d3b8-05f7-43c5-ad14-7d7ceee392fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4410, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004410__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4411-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4411, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4412, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4733", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4412", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5c1c4d5-379a-4ed4-ae62-bf7ad21b7e01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 382908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4411, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004411__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4412-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4412, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4413, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4734", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4413", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "afbf0bd3-52f8-470b-a978-889497f3e76b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4412, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004412__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4413-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4413, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4414, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4735", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4414", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8889489-27f2-4bff-a060-d0deda68ee23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4413, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004413__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4414-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4414, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "382953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4415, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4736", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4415", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a2763ac2-dc2f-4107-8bba-624b22cc63fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4414, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004414__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4415-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4415, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4416, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4737", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4416", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a07bc7a-f26d-4837-8ce9-30351382026e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4415, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004415__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4416-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4416, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4417, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4738", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4417", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d613aee-e06f-4480-987e-ba81a1b75368", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4416, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004416__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4417-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4417, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4418, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4739", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4418", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c3903e9-189c-4497-a43c-bea6c67fa9be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4417, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004417__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4418-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4418, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4419, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4740", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4419", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a155f061-bc50-40eb-adbd-0d426186bdd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4418, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004418__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4419-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4419, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4420, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4741", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4420", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a411b4ac-311c-4112-b2b4-b162ff60ff58", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4419, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004419__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4420-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4420, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4421, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4742", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4421", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5dc0fd64-73b0-4d4e-8a8e-61a0274e7487", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4420, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004420__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4421-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4421, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4422, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4743", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4422", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4aa3248d-a896-4597-9fe8-eac3135ca343", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4421, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004421__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4422-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4422, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4423, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4744", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4423", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ca89bbd-4eac-4083-a6c6-50a13ef38a71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4422, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004422__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4423-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4423, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4424, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4745", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4424", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "014d183b-9eeb-466c-85c3-ad2dab32a87a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 383955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4423, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004423__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4424-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4424, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4425, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4746", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4425", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13e02fb6-a05c-4a79-9ece-983cd90b57b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4424, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004424__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4425-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4425, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "383906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4426, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4747", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4426", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b21782c7-bfa9-46e2-afde-776133d5a879", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4425, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004425__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4426-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4426, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4427, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4748", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4427", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db86e21d-247c-40b5-9fc0-749ee246c20d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4426, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004426__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4427-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4427, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4428, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4749", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4428", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a86b2cf3-a330-4542-9eab-fe3ddbee49a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4427, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004427__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4428-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4428, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4429, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4750", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4429", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f295166-e22c-4a42-a328-b8f49f43bb4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4428, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004428__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4429-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4429, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4430, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4751", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4430", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21b20d88-0cfc-4547-ab53-8cf8a952dedd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4429, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004429__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4430-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4430, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4431, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4752", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4431", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d60f8414-36d0-481e-9569-98550fc92278", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4430, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004430__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4431-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4431, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4432, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4753", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4432", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bd18df7-ead9-4f91-8123-10d5a30e3786", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4431, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004431__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4432-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4432, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4433, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4754", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4433", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd7dddca-6d38-492a-8749-fd3a44bd626e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4432, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004432__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4433-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4433, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4434, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4755", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4434", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b5b40bd-2ce8-481e-8ad8-47e4c52cec53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4433, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004433__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4434-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4434, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4435, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4756", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4435", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52158a77-2763-4cd8-afed-d806e54aec99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 384908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4434, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004434__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4435-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4435, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4436, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4757", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4436", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc411ab7-2f5e-4281-9c0a-579b46e8ce10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4435, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004435__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4436-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4436, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4437, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4758", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4437", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9cf6095f-1a61-4b3d-8a86-5badceb228e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4436, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004436__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4437-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4437, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "384953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4438, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4759", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4438", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9c823ce-09d5-4eb1-afc3-19a1f99fd2d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4437, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004437__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4438-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4438, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4439, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4760", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4439", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76faea87-c24e-445b-b2a9-7017f8bad47e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4438, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004438__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4439-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4439, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4440, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4761", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4440", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "236a8b19-d4e8-42fd-a535-25f8489646da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4439, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004439__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4440-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4440, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4441, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4762", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4441", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79a3b6bf-1d14-47b8-9cc9-203e97be9e62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4440, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004440__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4441-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4441, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4442, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4763", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4442", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2285e264-72fe-43ce-a2c2-677db2c1c3ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4441, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004441__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4442-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4442, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4443, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4764", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4443", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "250daf2c-4dd0-4c55-97c0-805406aa82aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4442, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004442__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4443-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4443, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4444, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4765", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4444", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02089016-a979-4807-8dc9-291faa5ad852", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4443, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004443__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4444-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4444, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4445, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4766", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4445", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4cca42b-54d4-494e-b8f4-bf35aaca69cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4444, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004444__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4445-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4445, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4446, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4767", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4446", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "746994fd-2b4a-461b-a17a-1edef8107302", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4445, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004445__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4446-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4446, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4447, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4768", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4447", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9de1ecb7-5f9c-4494-a8be-4449614fd623", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 385955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4446, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004446__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4447-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4447, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4448, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4769", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4448", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43f6f8e7-244e-4560-a8c7-f7c364a80b32", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4447, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004447__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4448-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4448, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4449, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4770", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4449", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf018b71-0b03-4375-8fe7-fdcd7c9b7914", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4448, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004448__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4449-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4449, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "385984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4450, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4771", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4450", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "400416bc-13e7-47ca-8497-37cad2909d92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4449, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004449__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4450-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4450, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4451, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4772", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4451", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cedcaab-bb22-446e-a828-0f4e766a6c59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4450, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004450__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4451-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4451, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4452, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4773", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4452", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7470baaf-0fda-4e26-9d74-2e4907aa4830", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4451, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004451__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4452-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4452, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4453, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4774", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4453", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43398b73-b961-405d-8b8d-c67df199af76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4452, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004452__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4453-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4453, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4454, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4775", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4454", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1679968-04ea-49a7-ba0f-e356b4a65d41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4453, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004453__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4454-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4454, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4455, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4776", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4455", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3e83e36-385b-4a62-b08b-dfc2416cf90c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4454, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004454__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4455-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4455, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4456, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4777", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4456", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13327343-15a8-4dfd-9891-f2f0bab1b43e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4455, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004455__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4456-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4456, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4457, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4778", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4457", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fcd0d6f-a753-4cb3-8bc8-8649db785766", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4456, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004456__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4457-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4457, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4458, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4779", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4458", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "519f9771-6dbb-4a64-af75-a4dcdefab990", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4457, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004457__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4458-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4458, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4459, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4780", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4459", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d08ff79-e098-476a-8992-ff3a00169a59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 386986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4458, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004458__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4459-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4459, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4460, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4781", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4460", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6684325-460a-4187-a6c9-b2dac011fdb1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4459, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004459__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4460-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4460, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "386937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4461, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4782", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4461", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc3bdacf-5857-4cca-b479-b353c968db13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4460, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004460__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4461-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4461, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4462, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4783", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4462", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec7cb252-af8c-48b9-af5f-75804771c7ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4461, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004461__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4462-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4462, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4463, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4784", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4463", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e47fd221-abff-41cd-bc26-9901b5409e08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4462, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004462__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4463-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4463, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4464, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4785", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4464", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a55ce8a-3ace-48b1-b625-9a6770a2f3fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4463, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004463__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4464-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4464, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4465, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4786", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4465", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e20b8d7e-cc1f-470e-94ae-10fe032f45d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4464, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004464__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4465-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4465, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4466, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4787", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4466", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c07638d5-6415-4a1a-b0b4-401ba716b6b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4465, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004465__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4466-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4466, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4467, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4788", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4467", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a718b356-be9e-47a9-b264-30ab66ec8ba4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4466, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004466__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4467-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4467, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4468, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4789", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4468", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7d402da-33fe-40ad-947d-cd94644d2972", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4467, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004467__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4468-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4468, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4469, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4790", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4469", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4e534b6-eddf-428d-8975-8a1a110cbb20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4468, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004468__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4469-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4469, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4470, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4791", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4470", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ed18308-94a8-41c1-bb90-62d0403ffafb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 387940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4469, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004469__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4470-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4470, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4471, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4792", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4471", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "231881c9-90ca-44c8-8c5b-71451162d1b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4470, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004470__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4471-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4471, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4472, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4793", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4472", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4cd9679-fbbf-41ec-8b1d-ef0059569a28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4471, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004471__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4472-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4472, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "387984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4473, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4794", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4473", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ad1c3cb-1ec6-4b59-9033-f334542fdba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4472, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004472__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4473-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4473, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4474, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4795", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4474", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18acb906-f586-481e-86f1-02cc7682d3df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4473, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004473__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4474-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4474, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4475, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4796", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4475", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc6dfb18-6807-4bfd-9d2e-78514451eb1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4474, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004474__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4475-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4475, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4476, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4797", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4476", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8281a2c4-7bf2-4324-b71a-3718160159dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4475, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004475__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4476-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4476, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4477, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4798", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4477", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37d88599-bd99-4a7e-bd0e-1c8eeeeaccc7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4476, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004476__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4477-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4477, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4478, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4799", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4478", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4831fc8e-8edc-410f-b911-e32ecdf82b9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4477, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004477__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4478-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4478, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4479, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4800", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4479", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59e8dfed-d522-4091-a38b-7db485c8ea97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4478, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004478__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4479-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4479, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4480, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4801", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4480", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf14d49b-5238-47f8-aa60-8a309dfc2d3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4479, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004479__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4480-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4480, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4481, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4802", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4481", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f90fb5fd-37e4-4a05-bef9-6c87d49242ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4480, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004480__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4481-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4481, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4482, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4803", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4482", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d25b134e-7ff1-4b67-b0f9-be58d6728f8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 388986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4481, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004481__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4482-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4482, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4483, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4804", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4483", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29360b01-9f18-44e6-8e63-ff40a95156e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4482, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004482__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4483-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4483, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "388937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4484, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4805", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4484", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcf25f04-aff0-4ad3-b87e-e85ee7e90ef9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4483, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004483__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4484-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4484, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4485, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4806", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4485", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "860c16f0-b377-4148-b863-9d6392e1cc68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4484, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004484__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4485-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4485, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4486, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4807", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4486", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04d4befe-09a9-4144-8516-51bc2c41b96b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4485, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004485__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4486-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4486, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4487, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4808", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4487", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8bfcbd12-61c2-4218-bb43-c7c3413cb214", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4486, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004486__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4487-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4487, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4488, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4809", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4488", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4154d078-c5fd-4eda-93d1-28163da961bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4487, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004487__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4488-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4488, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4489, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4810", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4489", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ae84da1-174e-43fa-8633-2b4c972d93f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4488, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004488__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4489-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4489, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4490, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4811", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4490", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c0abad3-ea9b-4651-94d7-63405dd70df6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4489, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004489__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4490-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4490, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4491, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4812", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4491", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a62047d-4b18-4ba8-b41e-a088b77504a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4490, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004490__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4491-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4491, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4492, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4813", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4492", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9251ff29-1994-4b77-a265-910b159837ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4491, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004491__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4492-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4492, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4493, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4814", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4493", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5f6c48f-4689-4f9d-b84c-94bbbded1650", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 389940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4492, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004492__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4493-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4493, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4494, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4815", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4494", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4dce73a0-bbb2-47e4-81a7-ffd77f949df1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4493, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004493__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4494-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4494, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4495, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4816", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4495", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e77eb1c1-80e3-465b-b216-c63f18e48ed8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4494, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004494__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4495-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4495, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "389984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4496, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4817", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4496", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7adfb03b-9234-47fe-b6cc-9979db7fe48a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4495, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004495__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4496-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4496, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4497, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4818", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4497", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d81f6de0-0b77-467e-b146-7651d7694d7b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4496, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004496__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4497-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4497, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4498, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4819", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4498", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "584c836c-d223-41eb-b2c9-d48c1bd885bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4497, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004497__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4498-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4498, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4499, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4820", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4499", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4633835d-e254-4ad2-9c38-5baec0073bfc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4498, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004498__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4499-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4499, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4500, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4821", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4500", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c982a615-8cf4-491f-919a-a6bd193afe5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4499, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004499__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4500-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4500, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4501, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4822", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4501", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1bdd581-6c53-4861-a195-fc284c6025e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4500, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004500__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4501-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4501, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4502, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4823", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4502", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b9a4f33-e418-4356-a799-d0c309c108ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4501, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004501__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4502-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4502, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4503, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4824", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4503", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebafa2fd-90a2-45e5-895e-a8f0918b9156", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4502, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004502__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4503-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4503, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4504, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4825", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4504", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67007e26-8a97-4c19-9082-fc8d9d7c30c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4503, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004503__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4504-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4504, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4505, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4826", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4505", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a4a5ebb-4259-4a26-acab-ca9d2096ec61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4504, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004504__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4505-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4505, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4506, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4827", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4506", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6f69f0e-b3f8-4b40-96d4-47825483a552", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4505, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004505__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4506-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4506, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "390937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4507, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4828", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4507", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ef45b6b-846d-4ebd-baf2-338eda59b0dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4506, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004506__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4507-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4507, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4508, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4829", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4508", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99778bb1-33b4-450d-ad2d-3212bc1bb1b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4507, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004507__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4508-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4508, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4509, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4830", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4509", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f69d47b0-5122-43d1-8f22-5c53d1a32dad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4508, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004508__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4509-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4509, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4510, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4831", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4510", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cdb61f9-f92a-429b-8717-972d9ddf6f15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4509, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004509__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4510-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4510, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4511, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4832", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4511", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b52f0eff-3a43-4fb5-8f5f-19a86db21e69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4510, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004510__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4511-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4511, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4512, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4833", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4512", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "875fb0ef-0695-431c-9d35-5212c589092d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4511, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004511__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4512-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4512, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4513, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4834", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4513", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68885319-fa2a-495f-8aa6-a622cad84ef5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4512, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004512__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4513-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4513, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4514, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4835", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4514", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db5f5d15-737e-462a-81b9-e6a941fd2321", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4513, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004513__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4514-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4514, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4515, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4836", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4515", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6602dd73-e959-4ece-b3c0-c4adbcb18031", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4514, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004514__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4515-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4515, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4516, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4837", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4516", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b1c8752-375f-496d-85b3-bc148364a48a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 391940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4515, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004515__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4516-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4516, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4517, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4838", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4517", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3544034-861e-4c45-880b-e72296608af6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4516, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004516__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4517-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4517, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4518, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4839", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4518", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6b477f4-14c2-4150-be0c-ee65e0239858", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4517, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004517__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4518-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4518, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "391968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4519, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4840", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4519", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62bacab0-38d0-47d7-be65-c4d041f9364b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4518, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004518__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4519-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4519, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4520, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4841", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4520", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7155e36c-a156-4f27-a29b-3005e4891f27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4519, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004519__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4520-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4520, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4521, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4842", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4521", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25bb91ed-5b68-4592-b8a2-d59a83dfb34f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4520, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004520__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4521-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4521, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4522, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4843", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4522", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fba44d9-e493-47b2-b77e-d2986a7f13f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4521, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004521__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4522-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4522, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4523, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4844", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4523", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c51b4163-7c58-403a-9c16-bca661d34ecd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4522, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004522__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4523-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4523, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4524, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4845", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4524", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "881e0708-3ea6-4768-9a01-56943333fe85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4523, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004523__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4524-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4524, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4525, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4846", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4525", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e159a293-4b4d-45e5-99d4-3837488ffe66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4524, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004524__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4525-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4525, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4526, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4847", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4526", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a77baa63-adce-4f6a-8f2a-0f34eebb637f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4525, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004525__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4526-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4526, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4527, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4848", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4527", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30aaa5f8-2469-4b02-9569-2255ae7a0c27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4526, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004526__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4527-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4527, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4528, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4849", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4528", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ac9e669-0928-40b9-a115-79367bf53728", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 392971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4527, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004527__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4528-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4528, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4529, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4850", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4529", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3f97fa1-30fc-418a-b034-41ed2d659827", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4528, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004528__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4529-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4529, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "392921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4530, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4851", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4530", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "630fe036-a7ab-4315-983f-6c7dd3bc4295", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4529, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004529__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4530-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4530, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4531, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4852", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4531", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f44e1ec8-7551-4650-a9e9-5eea969b9988", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4530, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004530__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4531-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4531, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4532, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4853", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4532", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a7da446-e598-41ce-bdf3-ad448dfffd1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4531, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004531__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4532-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4532, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4533, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4854", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4533", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3e57cba-2cbc-41d6-a615-1b8d8867cc68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4532, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004532__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4533-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4533, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4534, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4855", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4534", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7873fce8-3958-404e-b86b-3821e18eaaec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4533, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004533__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4534-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4534, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4535, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4856", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4535", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf421ecb-643b-4db9-9b04-c121ea5f43c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4534, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004534__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4535-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4535, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4536, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4857", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4536", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7eb785b9-5ac9-43d7-a387-bf6f1fcab159", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4535, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004535__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4536-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4536, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4537, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4858", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4537", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9d34a5e-2f4d-43d0-ac43-e872f787a126", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4536, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004536__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4537-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4537, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4538, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4859", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4538", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d4945cf-b2d9-4c02-9255-dc41fa881a5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4537, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004537__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4538-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4538, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4539, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4860", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4539", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "077bfcff-3d6a-4c54-9f70-d3aefc9da3b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 393924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4538, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004538__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4539-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4539, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4540, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4861", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4540", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0bf5a35e-d87d-42f1-8c2a-d4576288de00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4539, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004539__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4540-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4540, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4541, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4862", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4541", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0eb7ce94-0ed6-4fc8-8c84-41f1ef00453f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4540, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004540__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4541-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4541, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "393968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4542, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4863", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4542", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41a1e366-ce40-4f78-b571-1ed68b7d2aa8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4541, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004541__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4542-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4542, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4543, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4864", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4543", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea176d72-1ce5-4469-b0d3-58b54d34d2e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4542, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004542__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4543-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4543, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4544, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4865", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4544", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7daa62b4-0ba5-49bb-a7f9-8c605209c1d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4543, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004543__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4544-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4544, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4545, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4866", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4545", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26a5761b-dbb8-4e52-a166-e40ae3898da5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4544, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004544__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4545-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4545, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4546, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4867", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4546", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc5aba9b-19ce-4150-a6dd-2163ab8ae767", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4545, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004545__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4546-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4546, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4547, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4868", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4547", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c436da1b-8518-4f78-b128-69d1cc53ad6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4546, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004546__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4547-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4547, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4548, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4869", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4548", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af9103ab-399e-42b4-969b-e0a5948e05e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4547, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004547__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4548-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4548, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4549, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4870", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4549", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d71dd88d-8aef-48f5-a7fa-7e7c28561dee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4548, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004548__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4549-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4549, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4550, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4871", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4550", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9df97ca5-ad2f-4a4d-9877-918dc7607cd0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4549, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004549__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4550-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4550, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4551, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4872", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4551", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "178b7a67-3db9-4e3d-bf1d-af10f2e57e96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 394971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4550, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004550__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4551-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4551, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4552, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4873", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4552", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa9e1d3e-139e-4a28-8a57-57c8be643561", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4551, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004551__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4552-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4552, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "394921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4553, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4874", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4553", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "781eb682-9e79-44e2-b5ae-964ac220164b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4552, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004552__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4553-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4553, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4554, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4875", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4554", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92c13309-a98c-435b-9a46-3c3147691712", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4553, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004553__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4554-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4554, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4555, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4876", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4555", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3acd3f5f-1739-480f-a728-f26ba7dc6d01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4554, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004554__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4555-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4555, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4556, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4877", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4556", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb7f8587-f425-42ed-aa74-7073b37753d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4555, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004555__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4556-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4556, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4557, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4878", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4557", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63a18904-a080-4d94-9f87-48d68940c539", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4556, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004556__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4557-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4557, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4558, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4879", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4558", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "292fcf56-c44f-4de2-841b-5e2c8c6989e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4557, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004557__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4558-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4558, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4559, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4880", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4559", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2aa188a-d9f2-4a12-97e6-2f7048658618", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4558, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004558__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4559-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4559, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4560, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4881", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4560", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4aaaf490-0703-4564-b643-19554f7fc9fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4559, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004559__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4560-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4560, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4561, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4882", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4561", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3752c9f-649f-450f-927f-32e76e4dc708", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4560, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004560__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4561-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4561, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4562, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4883", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4562", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96ea20a8-c643-46c1-8875-6acca2cdd851", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 395924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4561, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004561__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4562-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4562, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4563, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4884", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4563", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83c8f75e-b170-4046-b204-4318f7535dcd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4562, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004562__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4563-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4563, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4564, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4885", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4564", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb09e7f0-655d-41f9-82c1-5865d2048c41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4563, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004563__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4564-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4564, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "395968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4565, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4886", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4565", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d48817af-e073-4bac-8538-7e8f1e103570", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4564, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004564__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4565-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4565, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4566, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4887", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4566", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdb26764-7832-4480-bcca-a12247ebda6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4565, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004565__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4566-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4566, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4567, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4888", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4567", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9343ccd4-23dc-40bd-b5e5-c865e30a7956", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4566, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004566__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4567-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4567, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4568, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4889", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4568", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b3c4964-065a-4798-b18f-b67a11bb43be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4567, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004567__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4568-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4568, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4569, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4890", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4569", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c262f6f2-47c6-42b3-8be7-29814781c21f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4568, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004568__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4569-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4569, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4570, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4891", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4570", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75455059-13e4-4ae4-a7a5-b0667121db48", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4569, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004569__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4570-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4570, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4571, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4892", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4571", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c250f04-5567-45e0-be90-4342bac3fb7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4570, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004570__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4571-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4571, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4572, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4893", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4572", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "505affa0-a1e7-4381-811e-b173eb770fe6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4571, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004571__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4572-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4572, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4573, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4894", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4573", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60473db5-c179-492c-9616-93a67377c324", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4572, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004572__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4573-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4573, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4574, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4895", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4574", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b051fae0-b08e-4660-8732-9b31c45f9813", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 396971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4573, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004573__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4574-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4574, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4575, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4896", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4575", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14cc465c-ec4d-47fe-8750-9c88134c0005", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4574, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004574__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4575-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4575, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "396921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4576, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4897", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4576", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57dc0418-e419-448f-9877-a6ae5145981e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4575, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004575__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4576-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4576, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4577, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4898", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4577", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c89ad496-76f3-4170-8731-4e4c35080d21", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4576, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004576__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4577-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4577, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4578, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4899", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4578", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e075295a-3136-435a-b897-552faa9c9b1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4577, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004577__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4578-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4578, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4579, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4900", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4579", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80208945-dfbd-46e6-9353-a1da8a4e2153", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4578, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004578__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4579-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4579, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4580, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4901", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4580", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b32a2630-1fa5-4b09-9f63-1d7c3e3e2ec6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4579, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004579__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4580-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4580, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4581, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4902", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4581", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dab65226-3799-4441-9d4a-7d3b7ead1ec1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4580, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004580__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4581-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4581, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4582, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4903", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4582", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a442894e-5f3d-490d-8ce2-021f8fc8df49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4581, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004581__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4582-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4582, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4583, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4904", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4583", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a878c4af-bebd-467e-9434-6982c9c349bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4582, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004582__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4583-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4583, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4584, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4905", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4584", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf789682-ed0b-4a52-ad84-7b4a6b8a3e5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4583, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004583__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4584-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4584, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4585, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4906", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4585", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08c3415b-d369-4ca7-bee9-12808f36c51b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 397924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4584, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004584__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4585-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4585, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4586, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4907", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4586", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82de4d0d-d914-4195-8b84-7138c89fcca5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4585, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004585__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4586-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4586, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4587, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4908", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4587", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5775e405-8720-4c3f-95cc-4a3a3b905702", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4586, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004586__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4587-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4587, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "397953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4588, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4909", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4588", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77840625-940e-424c-8b21-f93169e9479c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4587, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004587__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4588-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4588, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4589, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4910", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4589", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45dd62c1-ed53-44b6-9f05-24bcb940e33e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4588, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004588__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4589-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4589, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4590, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4911", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4590", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75c1e4a6-a3de-437f-96cb-478d72f2b31e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4589, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004589__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4590-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4590, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4591, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4912", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4591", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e7d0a2d-3135-4972-9eeb-de16ea8fe7c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4590, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004590__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4591-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4591, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4592, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4913", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4592", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c4a838e-3463-4684-9dcd-d64919b29657", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4591, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004591__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4592-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4592, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4593, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4914", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4593", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f38bed3-ab03-4948-aa43-96332c4e6725", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4592, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004592__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4593-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4593, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4594, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4915", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4594", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23b57975-1b9b-46bd-91fa-6c1c8e5b6a12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4593, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004593__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4594-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4594, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4595, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4916", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4595", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ada4670-5694-4a4d-a429-e987aa58a126", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4594, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004594__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4595-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4595, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4596, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4917", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4596", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d05bd27d-7dfb-4b56-adf9-6ba70b31fce6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4595, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004595__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4596-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4596, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4597, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4918", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4597", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7970b300-13bb-4d34-9cf2-b36d9f17070b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 398955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4596, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004596__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4597-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4597, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4598, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4919", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4598", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a46ba038-5bf7-467f-96e0-93f201f9f671", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4597, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004597__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4598-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4598, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "398906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4599, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4920", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4599", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d167217-eb32-494c-97d4-ab03afc20301", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4598, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004598__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4599-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4599, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4600, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4921", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4600", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a2f37b7-950a-400f-9448-cf709528f94b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4599, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004599__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4600-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4600, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4601, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4922", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4601", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1873b924-edd5-4ec8-9fb5-6dd03e4eb9f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4600, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004600__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4601-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4601, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4602, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4923", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4602", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8130083b-251e-45d0-87d6-f824c7b0e417", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4601, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004601__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4602-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4602, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4603, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4924", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4603", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cc79e3d-1f89-43d4-a924-1ca678229cc5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4602, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004602__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4603-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4603, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4604, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4925", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4604", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48e08ad8-6762-4877-83ec-a8ffc52ad914", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4603, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004603__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4604-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4604, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4605, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4926", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4605", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f53aa79b-25c5-457b-afcb-8e6a8d119a73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4604, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004604__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4605-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4605, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4606, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4927", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4606", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "141d436c-f142-4351-b693-c6c4e44f4cb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4605, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004605__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4606-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4606, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4607, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4928", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4607", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "221f0d34-fac3-4a9f-9441-23a4bc85b16b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4606, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004606__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4607-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4607, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4608, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4929", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4608", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06405e3c-5672-4ea4-844b-477c91a8aa3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 399908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4607, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004607__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4608-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4608, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4609, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4930", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4609", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "102ca34a-00c6-433a-a43e-5165ca360a2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4608, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004608__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4609-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4609, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4610, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4931", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4610", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d713b30-2a89-4faa-ab62-81b76bc13451", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4609, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004609__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4610-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4610, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "399953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4611, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4932", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4611", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c8c3e21-cff9-46ae-93be-6dea2d78edac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4610, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004610__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4611-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4611, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4612, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4933", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4612", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9790e629-8c55-41f9-9a5f-8ea6c79153c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4611, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004611__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4612-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4612, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4613, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4934", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4613", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4992938-4337-4511-8ccc-6f4bb9862b7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4612, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004612__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4613-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4613, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4614, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4935", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4614", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e760452-fc67-4934-b8d3-9e524ff44eba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4613, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004613__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4614-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4614, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4615, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4936", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4615", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0950c5d1-c138-4d96-bcbf-52f6515d0234", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4614, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004614__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4615-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4615, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4616, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4937", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4616", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ac9b4f7-fa0f-40f8-8c06-70f10f427386", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4615, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004615__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4616-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4616, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4617, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4938", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4617", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54b8d505-8b44-4e5b-8772-ea03f387b16c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4616, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004616__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4617-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4617, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4618, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4939", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4618", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8236e59-fed2-4580-b716-0ae4388c733f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4617, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004617__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4618-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4618, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4619, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4940", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4619", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46ce7902-97cb-4b67-b52a-4a3e1ce64595", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4618, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004618__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4619-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4619, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4620, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4941", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4620", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9dfaab5-156e-490b-948a-8d504172335d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 400955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4619, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004619__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4620-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4620, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4621, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4942", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4621", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4da2de2-b452-47e6-8eac-d6c23dab7217", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4620, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004620__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4621-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4621, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "400906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4622, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4943", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4622", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86fb2ac6-60b4-4ecc-b7b1-b8e78c8315b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4621, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004621__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4622-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4622, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4623, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4944", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4623", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1c194de-dde8-4bc1-bf04-cf6215abda3c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4622, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004622__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4623-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4623, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4624, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4945", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4624", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6907f21-8b94-48ac-a38e-9dac39beaa5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4623, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004623__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4624-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4624, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4625, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4946", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4625", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7983619-da32-49de-9c34-03d7617d8794", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4624, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004624__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4625-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4625, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4626, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4947", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4626", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cc08e52-739b-4013-b36b-881d9067554d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4625, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004625__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4626-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4626, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4627, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4948", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4627", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ae0bbc3-5ecc-493f-98d7-24688f21bc34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4626, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004626__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4627-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4627, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4628, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4949", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4628", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c69d8366-5776-4dbb-866a-2e0c549fa7ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4627, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004627__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4628-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4628, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4629, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4950", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4629", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec4df4b6-ced6-4f78-8b64-30bde5563abd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4628, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004628__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4629-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4629, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4630, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4951", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4630", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "feb68a56-e8fc-44ec-85b5-f54ccf166fd6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4629, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004629__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4630-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4630, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4631, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4952", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4631", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "176c918f-6e94-49e0-8bbc-b6ab989cf0de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 401908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4630, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004630__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4631-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4631, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4632, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4953", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4632", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ceb79d33-5d39-4b08-966b-a14c672eb621", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4631, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004631__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4632-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4632, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4633, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4954", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4633", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a22fe77-f367-4496-bfbd-1138e371af4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4632, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004632__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4633-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4633, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "401953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4634, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4955", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4634", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ffa5b642-05e1-4f48-839a-4c74e7dd0313", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4633, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004633__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4634-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4634, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4635, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4956", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4635", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "edad6dc8-5e5e-405d-a9ea-d6e84377a05b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4634, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004634__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4635-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4635, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4636, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4957", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4636", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "248d33a6-200a-44a3-a331-d9c83e11a1e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4635, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004635__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4636-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4636, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4637, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4958", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4637", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b36fb6b-20e5-4a22-b1f5-5c7e6ecf7e4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4636, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004636__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4637-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4637, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4638, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4959", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4638", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60d0dde4-29db-4c95-bfca-081e7d3aceac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4637, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004637__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4638-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4638, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4639, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4960", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4639", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb0cd8ad-14f9-4a3e-ab11-c816179c95b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4638, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004638__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4639-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4639, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4640, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4961", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4640", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4580456-416c-4215-8fd3-00c4596ae776", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4639, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004639__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4640-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4640, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4641, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4962", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4641", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96751594-b1b1-43fc-9451-a4491f9c1792", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4640, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004640__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4641-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4641, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4642, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4963", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4642", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3e2c508-b0c9-4961-8f01-50d99f6a8bea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4641, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004641__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4642-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4642, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4643, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4964", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4643", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2139c689-2797-4cbc-9028-e63721c6b65b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 402955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4642, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004642__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4643-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4643, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4644, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4965", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4644", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "463b3cb3-084d-4720-9961-01da07cd9a93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4643, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004643__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4644-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4644, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4645, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4966", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4645", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "147d9c81-6928-42a4-9ca2-b3329d3b1149", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4644, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004644__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4645-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4645, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "402984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4646, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4967", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4646", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "759438ff-de49-48a5-8fe2-bb86b51b5071", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4645, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004645__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4646-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4646, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4647, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4968", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4647", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03c8e623-d42d-4766-9b7d-3c080f62730b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4646, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004646__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4647-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4647, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4648, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4969", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4648", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f000ac8-e517-4acc-bdd2-dfee4f058fb8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4647, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004647__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4648-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4648, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4649, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4970", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4649", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fdba40cc-9d00-4ad1-b760-29c1592723f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4648, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004648__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4649-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4649, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4650, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4971", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4650", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46581547-bc66-492e-bc71-d9c22ef7a495", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4649, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004649__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4650-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4650, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4651, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4972", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4651", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38e1fa02-29d2-423d-a504-9b3c53bb84f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4650, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004650__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4651-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4651, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4652, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4973", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4652", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e6a7cad-5833-4914-a6b7-9602e44f58ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4651, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004651__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4652-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4652, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4653, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4974", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4653", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "024086ce-9b06-4d97-9ec2-3750b7051aff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4652, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004652__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4653-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4653, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4654, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4975", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4654", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1c28e76-3062-4f49-a591-a38f62dd0485", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4653, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004653__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4654-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4654, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4655, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4976", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4655", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea827e10-7327-45fc-9795-be58e28a4822", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 403986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4654, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004654__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4655-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4655, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4656, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4977", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4656", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7cbab5f3-75a7-4cf5-a45f-228bb8063a77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4655, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004655__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4656-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4656, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "403937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4657, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4978", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4657", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15bb5fef-a23c-4dab-90f2-bef6185852fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4656, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004656__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4657-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4657, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4658, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4979", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4658", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40ea10f9-4ced-487b-8782-2e5320cfbe0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4657, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004657__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4658-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4658, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4659, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4980", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4659", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d5c1aa9-b3d8-47d8-bf64-149e6aa83b0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4658, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004658__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4659-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4659, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4660, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4981", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4660", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b047399-2576-484f-b442-1b6750cf9737", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4659, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004659__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4660-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4660, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4661, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4982", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4661", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d22b91d-4e0f-4ccd-b705-08965dd21e5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4660, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004660__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4661-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4661, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4662, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4983", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4662", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c44329e9-5345-4d79-8a85-b8b6fd089f11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4661, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004661__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4662-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4662, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4663, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4984", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4663", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "432e5d05-4873-4741-8146-a0b8b4d48e60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4662, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004662__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4663-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4663, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4664, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4985", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4664", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4503c26e-ef58-4975-8c4f-9522a778f0ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4663, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004663__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4664-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4664, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4665, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4986", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4665", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1cc2865a-bf5e-4baa-97ba-a46895ee3318", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4664, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004664__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4665-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4665, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4666, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4987", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4666", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efc31121-27cb-4858-a80b-b6972199b838", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 404940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4665, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004665__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4666-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4666, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4667, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4988", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4667", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07f714b3-887d-4ddc-ab23-e16635cd9369", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4666, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004666__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4667-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4667, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4668, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4989", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4668", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcce5781-a69b-4349-8ef6-d4bfa5e10f41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4667, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004667__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4668-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4668, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "404984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4669, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4990", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4669", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd076738-2855-4289-868a-0ed23ec1e20c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4668, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004668__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4669-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4669, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4670, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4991", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4670", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56882f1c-c347-4a96-b649-739888ef2b9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4669, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004669__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4670-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4670, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4671, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4992", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4671", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6676fd19-3155-46bf-920f-cc40f38593d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4670, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004670__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4671-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4671, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4672, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4993", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4672", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68d184da-ef4f-40e6-b918-0079722abef2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4671, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004671__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4672-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4672, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4673, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4994", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4673", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5eca5d5d-2053-49af-80ba-43626a028e0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4672, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004672__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4673-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4673, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4674, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4995", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4674", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa23c410-7ba4-4c05-858c-6452ebfa3150", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4673, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004673__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4674-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4674, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4675, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4996", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4675", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1df7a206-8dcc-45a7-8272-ee1c3a068b92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4674, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004674__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4675-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4675, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4676, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4997", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4676", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ded929e-4847-4915-ad84-9f109570d336", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4675, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004675__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4676-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4676, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4677, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4998", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4677", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "050ad1f5-6301-4d23-b999-0b2e31f504ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4676, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004676__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4677-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4677, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4678, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "4999", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4678", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0981b491-73c1-4a80-bc62-37cb97a278df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 405986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4677, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004677__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4678-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4678, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4679, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5000", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4679", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16080898-7e20-4f27-aa04-4c5bc1bcc91d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4678, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004678__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4679-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4679, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "405937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4680, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5001", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4680", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb7536a5-ea8c-4304-858a-d7036fac3200", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4679, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004679__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4680-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4680, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4681, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5002", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4681", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00c3da94-f29e-462b-9414-97f4cf372d90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4680, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004680__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4681-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4681, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4682, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5003", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4682", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8563a2ea-1da8-44a2-a173-4ab1b13cb652", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4681, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004681__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4682-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4682, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4683, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5004", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4683", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3145e03-64b3-4414-aafe-8ea3f3eda112", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4682, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004682__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4683-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4683, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4684, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5005", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4684", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ac08f2d-77d3-4992-b8ba-ccc431a6b98f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4683, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004683__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4684-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4684, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4685, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5006", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4685", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5f0d639-c050-4857-901f-73bb4603b904", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4684, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004684__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4685-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4685, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4686, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5007", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4686", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b20acbe-70cf-47c0-a325-fc6cb60e2b45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4685, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004685__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4686-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4686, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4687, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5008", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4687", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d014ffc6-8229-459b-9c5e-1ed60294691f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4686, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004686__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4687-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4687, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4688, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5009", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4688", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f173621-4046-4cba-9a16-5330ff34d837", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4687, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004687__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4688-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4688, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4689, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5010", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4689", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ff5d26a-bac3-49f2-99b0-5f27b10f2c87", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 406940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4688, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004688__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4689-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4689, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4690, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5011", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4690", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd244ae7-8c6c-45cc-b6fe-1d4892f6f29d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4689, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004689__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4690-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4690, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4691, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5012", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4691", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d8ed61b-ac36-4e60-9b4f-a7ee235ef61f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4690, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004690__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4691-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4691, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "406984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4692, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5013", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4692", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1f44119-35f9-482e-b36c-3a29f7d582b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4691, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004691__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4692-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4692, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4693, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5014", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4693", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee2e8a69-bfe8-4a23-aa01-b93c8ccc10b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4692, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004692__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4693-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4693, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4694, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5015", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4694", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec990817-4f82-4842-9c15-0d238eed6b61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4693, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004693__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4694-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4694, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4695, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5016", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4695", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4328d56-9b93-4b2b-8485-c51803eb80f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4694, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004694__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4695-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4695, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4696, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5017", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4696", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "401bbde3-e38b-44cb-899f-72a7e0b30de2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4695, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004695__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4696-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4696, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4697, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5018", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4697", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8623e36-c05d-4d78-bcd2-b53c289c3040", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4696, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004696__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4697-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4697, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4698, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5019", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4698", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8eaf029e-6df7-4822-b122-de208759f247", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4697, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004697__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4698-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4698, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4699, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5020", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4699", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c09c9c0b-f7c8-45d8-beaa-b1fbf45c2e54", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4698, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004698__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4699-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4699, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4700, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5021", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4700", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e3d4a95-933b-4cbc-a5bc-6d0633025679", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4699, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004699__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4700-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4700, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4701, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5022", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4701", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17dba9c6-7e53-45be-95e1-9278150463d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 407986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4700, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004700__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4701-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4701, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4702, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5023", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4702", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2504f852-49f0-40a4-b21c-52d2751acdfb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4701, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004701__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4702-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4702, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "407937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4703, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5024", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4703", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1510c244-a314-4c96-bd6f-2bcc876f707b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4702, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004702__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4703-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4703, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4704, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5025", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4704", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "782b25cb-d9fa-45ee-84e2-085c2f368b24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4703, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004703__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4704-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4704, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4705, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5026", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4705", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a3aea78-a9f0-42c7-bad7-e50e66b31c26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4704, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004704__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4705-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4705, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4706, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5027", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4706", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57fb4841-d3c7-45c7-82c3-0b7483701986", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4705, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004705__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4706-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4706, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4707, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5028", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4707", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b2411d6-0e85-409a-8da5-cbf9e5df41eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4706, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004706__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4707-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4707, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4708, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5029", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4708", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e4f0654-cddd-4b6e-865d-23619b72a694", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4707, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004707__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4708-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4708, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4709, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5030", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4709", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25886470-361f-49b1-a857-676044095266", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4708, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004708__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4709-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4709, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4710, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5031", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4710", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85eec610-9a85-4b3e-83f4-000d3d950fa7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4709, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004709__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4710-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4710, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4711, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5032", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4711", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba69e4ac-41a9-4295-bdfc-e252b3d9f96b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4710, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004710__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4711-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4711, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4712, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5033", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4712", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6592200f-aef1-49a9-a699-f5d5bfd0656d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 408940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4711, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004711__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4712-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4712, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4713, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5034", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4713", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62bbfd10-66d4-497b-bc87-34d7694e4059", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4712, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004712__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4713-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4713, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4714, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5035", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4714", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c034948-901b-4e92-9991-564c6d6c5797", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4713, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004713__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4714-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4714, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "408968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4715, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5036", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4715", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af98509f-971d-4154-8260-f96741488646", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4714, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004714__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4715-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4715, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4716, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5037", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4716", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "380dd733-645c-4fe4-b9e4-84597bac5a87", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4715, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004715__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4716-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4716, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4717, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5038", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4717", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8f41368-1a0c-40e0-a6de-4be313a511c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4716, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004716__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4717-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4717, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4718, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5039", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4718", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc515398-4c31-49ba-bdf7-b12a73375515", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4717, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004717__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4718-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4718, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4719, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5040", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4719", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d60cafb7-e908-481e-9529-5be8b9c6dee5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4718, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004718__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4719-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4719, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4720, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5041", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4720", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8187453-12e9-4099-9dc5-3ea1ae585a14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4719, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004719__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4720-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4720, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4721, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5042", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4721", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4b5eb03-b547-4f3c-85ff-36c39cb6866f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4720, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004720__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4721-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4721, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4722, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5043", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4722", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64631ec4-a8e5-4f57-93d0-59c08ec9d961", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4721, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004721__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4722-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4722, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4723, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5044", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4723", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3272ee9e-f4fd-40bc-85d1-7d62a07f1abc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4722, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004722__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4723-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4723, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4724, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5045", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4724", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f826ad1-4c0b-4411-88ae-6bf9c3644a5a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 409971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4723, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004723__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4724-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4724, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4725, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5046", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4725", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9113309-a759-4aad-bead-5cede376a3fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4724, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004724__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4725-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4725, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "409921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4726, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5047", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4726", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef988e3a-3f6f-4dd2-ba20-08f6ae98c0a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4725, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004725__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4726-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4726, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4727, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5048", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4727", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86804808-6cb0-41eb-8d75-8d6ae076ab6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4726, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004726__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4727-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4727, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4728, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5049", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4728", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea76d96a-e979-4b12-9bab-177bd8cb9dc4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4727, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004727__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4728-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4728, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4729, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5050", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4729", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "362b9189-b023-4e25-a2e6-42f5556c16a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4728, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004728__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4729-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4729, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4730, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5051", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4730", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc454d78-e586-4d2d-bb7d-5072797aefd2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4729, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004729__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4730-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4730, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4731, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5052", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4731", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e444fbf-40d6-4b98-9363-d94bfbb30ae3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4730, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004730__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4731-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4731, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4732, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5053", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4732", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19a9c89e-3f24-4bd8-a2fa-8f697ed401e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4731, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004731__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4732-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4732, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4733, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5054", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4733", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f1800cb-dc90-4821-a39f-2acdfdfbaeca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4732, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004732__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4733-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4733, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4734, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5055", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4734", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95eb44bc-1bb4-4a12-93a9-e39c12946830", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4733, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004733__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4734-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4734, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4735, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5056", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4735", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7efb1f5b-6530-4999-a513-e0a1e2c27f44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 410924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4734, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004734__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4735-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4735, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4736, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5057", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4736", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e124d536-d068-4c3b-a667-e17858964e44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4735, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004735__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4736-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4736, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4737, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5058", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4737", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "130645f0-85e7-41db-bc72-0dc72918535e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4736, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004736__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4737-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4737, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "410968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4738, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5059", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4738", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "957d0ed4-5ebf-49b3-a5b3-e996724fd6a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4737, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004737__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4738-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4738, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4739, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5060", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4739", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a056b0d-bce9-4650-a615-c97a2278a78d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4738, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004738__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4739-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4739, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4740, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5061", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4740", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9ae9ccf-ca52-4a94-a858-bbe1ab50eda5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4739, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004739__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4740-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4740, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4741, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5062", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4741", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2c63fe7-9b9a-4cc4-984a-ebb199f77b29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4740, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004740__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4741-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4741, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4742, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5063", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4742", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ac9ab33-828b-4403-9308-28fbff737617", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4741, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004741__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4742-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4742, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4743, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5064", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4743", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adf2b542-135d-4c7a-8760-c33bd9de7558", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4742, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004742__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4743-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4743, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4744, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5065", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4744", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80c30c5a-21c8-4b4f-8d13-79de0f12e698", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4743, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004743__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4744-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4744, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4745, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5066", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4745", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d56cf27-3d34-43fe-9e5b-f9fc856ea295", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4744, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004744__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4745-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4745, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4746, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5067", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4746", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45245e19-95af-4704-9a0f-c0e80c1f1f15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4745, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004745__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4746-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4746, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4747, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5068", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4747", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9bb00f2-2673-4dbf-afdd-91b418abe3ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 411971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4746, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004746__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4747-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4747, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4748, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5069", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4748", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c636f8f-417b-4192-8019-4848d295c923", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4747, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004747__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4748-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4748, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "411921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4749, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5070", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4749", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4f95f45-6cdd-4dd5-a473-0189b567e227", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4748, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004748__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4749-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4749, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4750, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5071", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4750", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b803606d-73cd-4d4e-abbd-e2a917961f73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4749, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004749__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4750-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4750, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4751, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5072", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4751", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcb10efc-95b1-4b2c-ae0f-f6e9fd9672e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4750, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004750__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4751-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4751, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4752, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5073", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4752", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc7aa5a6-b46f-4590-9f54-3b46ee935705", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4751, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004751__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4752-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4752, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4753, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5074", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4753", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ed8b492-965b-4676-ae27-60ba3b07fef3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4752, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004752__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4753-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4753, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4754, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5075", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4754", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c9024f0-e276-4775-a1ac-900f05c92af5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4753, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004753__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4754-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4754, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4755, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5076", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4755", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2685e46f-34ae-4f7f-8e09-003e8264f262", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4754, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004754__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4755-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4755, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4756, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5077", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4756", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "054ac5e2-438d-4401-bc5e-0bbc99d79702", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4755, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004755__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4756-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4756, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4757, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5078", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4757", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5e95b5c-b001-4111-a014-e922da274766", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4756, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004756__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4757-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4757, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4758, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5079", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4758", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab3238fe-8f2e-4677-9f29-92a9026dd0db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 412924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4757, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004757__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4758-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4758, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4759, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5080", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4759", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0951b3a0-7437-49e0-9079-8b84d80cd3a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4758, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004758__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4759-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4759, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4760, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5081", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4760", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b37029f-2db3-4bcd-8f6a-17d8c264ace5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4759, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004759__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4760-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4760, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "412968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4761, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5082", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4761", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4bd8944-0c13-4775-a856-e62cfdffd2ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4760, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004760__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4761-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4761, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4762, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5083", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4762", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f055de8a-ed79-45b4-bcf0-83d2b43e71c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4761, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004761__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4762-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4762, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4763, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5084", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4763", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2213afd1-ccbd-4a35-8fdf-521ea9103205", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4762, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004762__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4763-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4763, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4764, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5085", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4764", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9b26ba7-92c0-455a-8c92-fc9cc9fa6d71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4763, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004763__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4764-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4764, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4765, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5086", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4765", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a051c56-3f3c-4cc3-8aa5-c4a29c840f49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4764, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004764__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4765-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4765, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4766, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5087", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4766", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6275a7aa-a281-4d0d-99f6-f6bc8156a0f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4765, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004765__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4766-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4766, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4767, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5088", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4767", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9ee6bf6-9ede-4ffe-94e3-1fa8e8e29ba3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4766, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004766__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4767-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4767, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4768, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5089", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4768", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11399074-da3e-4e8b-abf8-55241eb43b3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4767, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004767__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4768-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4768, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4769, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5090", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4769", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "660653db-0e29-45e7-b821-314c7986a47c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4768, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004768__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4769-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4769, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4770, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5091", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4770", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e0de4d8-3510-47f0-a3bb-d98f84a0a3ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 413971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4769, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004769__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4770-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4770, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4771, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5092", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4771", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c128e12-b129-41ee-8a16-a34f7d475b51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4770, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004770__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4771-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4771, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "413921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4772, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5093", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4772", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb618215-4d0a-4b3f-9452-46cb7b238fd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4771, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004771__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4772-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4772, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4773, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5094", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4773", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbdd9069-38ea-456b-9079-264bfacb9b82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4772, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004772__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4773-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4773, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4774, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5095", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4774", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3af76613-dab4-4022-ac61-25d42478d708", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4773, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004773__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4774-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4774, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4775, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5096", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4775", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50ee09b2-b06e-4fe0-92b8-a60883b07b52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4774, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004774__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4775-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4775, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4776, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5097", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4776", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44173b43-7d70-4d0d-baa1-a3ffb9f25e3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4775, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004775__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4776-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4776, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4777, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5098", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4777", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad083c17-9e27-4ff5-b6a5-0b4356cd0db6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4776, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004776__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4777-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4777, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4778, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5099", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4778", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1823495-afd7-437e-8ad4-ee717cc2c228", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4777, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004777__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4778-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4778, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4779, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5100", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4779", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e2541df-9fba-4b41-b54d-f9f813733125", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4778, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004778__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4779-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4779, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4780, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5101", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4780", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ec39306-9a96-4bbb-8ad0-8e12f5402dd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4779, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004779__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4780-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4780, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4781, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5102", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4781", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3af6e02f-6edd-4cf8-8f11-43e56ff055d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 414924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4780, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004780__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4781-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4781, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4782, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5103", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4782", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df53e362-51f9-44cb-8f47-7daaa20a20f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4781, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004781__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4782-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4782, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4783, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5104", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4783", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55ceb54a-c01c-4162-8f0e-199e6614c400", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4782, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004782__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4783-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4783, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "414953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4784, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5105", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4784", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9cf35e0-df93-48a3-ae78-8b8af4c08872", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4783, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004783__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4784-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4784, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4785, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5106", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4785", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1007b9e-e83d-4637-8f21-10d78c6bcd7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4784, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004784__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4785-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4785, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4786, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5107", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4786", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58b8841c-270f-4f16-af1d-5c0e2a0b9131", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4785, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004785__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4786-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4786, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4787, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5108", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4787", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1089c072-d3c7-4d08-a3a3-9185835cc6d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4786, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004786__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4787-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4787, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4788, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5109", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4788", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c8b0c40-1fa0-436f-96ac-4497f8e94c73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4787, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004787__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4788-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4788, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4789, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5110", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4789", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c097f62b-eed7-41c5-a3c4-f13b09412d96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4788, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004788__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4789-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4789, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4790, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5111", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4790", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05207261-1ff7-44b0-a7cc-e6213e6ed891", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4789, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004789__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4790-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4790, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4791, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5112", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4791", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09779863-bbc8-42db-9d84-ff5691c70ae6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4790, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004790__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4791-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4791, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4792, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5113", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4792", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4345d2c-eb1b-4cc9-a593-178f94a78d4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4791, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004791__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4792-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4792, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4793, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5114", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4793", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2bffff12-ef07-4abb-a5b2-364cfbf705bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 415955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4792, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004792__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4793-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4793, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4794, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5115", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4794", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8dd6a6e1-81fb-408e-9126-567ee4ab5056", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4793, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004793__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4794-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4794, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "415906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4795, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5116", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4795", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d05be74a-6ffe-4677-b58e-77aef31b0f13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4794, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004794__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4795-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4795, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4796, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5117", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4796", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "933f1d97-dacb-4fa4-961a-a7de45e3ee18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4795, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004795__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4796-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4796, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4797, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5118", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4797", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "120f8dbe-2805-4e67-bd1f-ff3e8d4edb71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4796, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004796__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4797-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4797, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4798, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5119", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4798", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7642659-e837-4129-a939-c0e2cb7317d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4797, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004797__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4798-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4798, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4799, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5120", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4799", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "116d4767-bc64-4fa4-9abb-4d06b51097bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4798, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004798__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4799-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4799, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4800, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5121", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4800", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2e4e066-e807-4f7e-bbfb-94b7b624c7ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4799, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004799__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4800-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4800, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4801, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5122", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4801", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24010d5a-c637-4aac-ac44-5460f2b0712e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4800, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004800__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4801-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4801, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4802, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5123", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4802", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e88a3f9f-febf-4743-9892-e34b7d29fc49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4801, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004801__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4802-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4802, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4803, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5124", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4803", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "723304f2-736a-4de7-84d7-99bd4eaee60a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4802, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004802__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4803-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4803, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4804, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5125", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4804", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "856b9b4a-e311-444a-841f-49cf8ebfa34c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 416908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4803, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004803__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4804-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4804, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4805, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5126", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4805", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e1769e6-0070-473d-9aab-d212756aaade", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4804, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004804__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4805-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4805, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4806, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5127", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4806", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab494996-f7fc-4cca-af39-b8b60880d28f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4805, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004805__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4806-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4806, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "416953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4807, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5128", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4807", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89dfd69e-b2b3-42b2-80af-6d3304de525e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4806, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004806__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4807-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4807, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4808, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5129", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4808", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62c95523-3143-42d1-b277-e6313bd42a29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4807, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004807__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4808-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4808, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4809, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5130", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4809", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13b9c3bf-c9c3-4529-bd1f-2074266c3a89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4808, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004808__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4809-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4809, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4810, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5131", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4810", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df0e36cd-c883-4ba5-97c6-56471c7a3040", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4809, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004809__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4810-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4810, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4811, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5132", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4811", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20f04699-4e33-431c-99fd-300d5e7659ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4810, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004810__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4811-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4811, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4812, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5133", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4812", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e53f12fa-5a51-47bf-a5de-a211f8e9bcfc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4811, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004811__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4812-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4812, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4813, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5134", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4813", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44b44b58-672b-4905-b2a9-505872bc61b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4812, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004812__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4813-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4813, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4814, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5135", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4814", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f541017-c251-4258-90ea-f0802f7d9e17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4813, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004813__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4814-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4814, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4815, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5136", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4815", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20aaa099-488b-4a9d-8a91-65e158aca72a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4814, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004814__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4815-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4815, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4816, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5137", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4816", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a13b48d-7b0b-4d3c-901b-94b25cbace73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 417955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4815, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004815__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4816-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4816, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4817, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5138", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4817", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26f3626b-bda9-4bfa-bf16-0d4faad3e3f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4816, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004816__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4817-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4817, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "417906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4818, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5139", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4818", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d4a6690-0ccf-44c1-985f-8f0449183301", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4817, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004817__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4818-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4818, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4819, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5140", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4819", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "300ce080-5ff7-46d9-8fb2-0265cbac8efd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4818, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004818__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4819-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4819, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4820, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5141", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4820", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d21efc4-2915-4acf-94ca-c8baf95cce52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4819, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004819__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4820-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4820, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4821, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5142", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4821", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adff1b0f-c98c-4e48-9a2c-fb011cd33657", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4820, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004820__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4821-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4821, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4822, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5143", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4822", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e4fbc16-794b-44b1-b6d1-33de29618113", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4821, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004821__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4822-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4822, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4823, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5144", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4823", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc0da729-96fb-4625-a161-9d48da55f029", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4822, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004822__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4823-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4823, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4824, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5145", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4824", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69f0a830-6cd8-44c3-b55e-3bd68c6220de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4823, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004823__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4824-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4824, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4825, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5146", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4825", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "734c4435-d2fa-4cd8-9ed3-71d75de42d59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4824, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004824__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4825-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4825, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4826, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5147", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4826", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "729b989e-57ea-4081-8812-976100d9a344", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4825, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004825__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4826-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4826, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4827, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5148", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4827", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62667352-ffeb-454b-b1d6-3e96819e4d8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 418908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4826, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004826__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4827-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4827, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4828, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5149", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4828", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a45481c-6553-4332-b68e-5a3b525f85cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4827, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004827__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4828-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4828, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4829, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5150", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4829", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88bf2662-e0e0-481f-97e5-d63f475fe2a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4828, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004828__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4829-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4829, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "418953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4830, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5151", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4830", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83d8a647-fc25-41ff-bcbb-afc1f6f20c95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4829, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004829__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4830-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4830, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4831, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5152", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4831", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4853e0e7-bbc9-4fdc-be3d-e1297875e368", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4830, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004830__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4831-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4831, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4832, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5153", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4832", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5991af47-6314-46d0-9406-2c9ca3d78727", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4831, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004831__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4832-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4832, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4833, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5154", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4833", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24a2ca65-2466-49d5-ba30-1c6bb57e7f01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4832, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004832__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4833-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4833, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4834, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5155", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4834", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58d12eb2-0439-4eed-bcb2-3f8e26adf26d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4833, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004833__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4834-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4834, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4835, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5156", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4835", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5032dd3-4985-45be-afe5-4b4927cd0627", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4834, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004834__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4835-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4835, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4836, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5157", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4836", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0b72a2d-6a66-4910-96b3-10881b36037f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4835, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004835__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4836-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4836, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4837, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5158", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4837", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c584e63-ed7b-45fa-b810-8ff188211b4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4836, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004836__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4837-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4837, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4838, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5159", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4838", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe3a2cd8-7753-44d6-8eaa-ffb12ed63055", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4837, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004837__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4838-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4838, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4839, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5160", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4839", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70402178-a1c2-47ec-805d-0f6e7300147c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 419955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4838, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004838__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4839-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4839, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4840, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5161", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4840", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a39073c2-c18f-4714-a119-e088b0187c50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4839, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004839__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4840-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4840, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4841, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5162", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4841", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01163db2-cd0e-4fa5-9b78-1d39bdb270c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4840, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004840__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4841-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4841, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "419984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4842, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5163", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4842", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99a69c51-2a9d-4704-b70c-f1bcc3c8bf1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4841, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004841__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4842-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4842, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4843, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5164", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4843", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e5a6826-a65a-4fde-b824-2fbaac375811", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4842, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004842__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4843-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4843, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4844, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5165", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4844", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df199dcf-98dd-4299-832f-7afcfd00fc9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4843, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004843__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4844-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4844, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4845, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5166", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4845", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b0ed249-ba1a-48ae-aa66-8df9f149670f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4844, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004844__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4845-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4845, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4846, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5167", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4846", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80bece58-0951-48ed-be6a-03ac52114f58", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4845, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004845__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4846-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4846, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4847, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5168", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4847", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd6d19cb-f0d8-4aaf-b426-bc18ce645a04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4846, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004846__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4847-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4847, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4848, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5169", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4848", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f249ddf-0444-43c6-992c-f223e7a89bc8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4847, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004847__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4848-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4848, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4849, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5170", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4849", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a37c5eb8-4aa4-4aa4-8526-b94f603c3e1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4848, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004848__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4849-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4849, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4850, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5171", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4850", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98ce782f-67f3-43b6-b21e-18b2a6ca763c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4849, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004849__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4850-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4850, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4851, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5172", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4851", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68b82b65-390a-4f09-9ac2-111d8e92a413", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 420986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4850, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004850__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4851-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4851, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4852, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5173", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4852", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abd77968-2ac7-4a99-949a-e38408d6d90e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4851, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004851__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4852-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4852, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "420937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4853, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5174", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4853", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dce857f8-8c72-4eee-8645-a77314e0a548", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4852, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004852__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4853-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4853, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4854, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5175", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4854", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ae8d789-9821-4411-9191-49ec4bcb02b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4853, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004853__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4854-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4854, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4855, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5176", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4855", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "051ab384-3ee8-461d-8705-6c19c08d0d25", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4854, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004854__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4855-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4855, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4856, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5177", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4856", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0c0870e-29a1-44b3-85ee-3f073510bbe8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4855, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004855__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4856-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4856, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4857, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5178", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4857", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a714b71-0bf3-4b13-8ae1-6a7ec1130eaa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4856, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004856__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4857-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4857, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4858, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5179", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4858", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ea549cb-70be-4bed-a3ba-8b69c0a582ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4857, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004857__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4858-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4858, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4859, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5180", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4859", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d0c2d24-e46b-40a2-b9cf-00b0d1cc9a32", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4858, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004858__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4859-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4859, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4860, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5181", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4860", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25879937-51c2-4287-b199-c638abbbb28b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4859, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004859__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4860-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4860, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4861, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5182", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4861", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e372516-68f3-40a2-9556-c3373cee6002", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4860, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004860__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4861-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4861, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4862, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5183", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4862", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98713e4d-4439-482b-a247-02253c74b60b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 421940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4861, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004861__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4862-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4862, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4863, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5184", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4863", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "350adac3-2167-4071-88d3-cbcdaaadff7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4862, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004862__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4863-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4863, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4864, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5185", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4864", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f32d769-2b8f-4b24-a99a-63a7332798c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4863, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004863__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4864-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4864, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "421984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4865, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5186", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4865", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95314de7-13f5-4c74-8a87-2d3ec5cf2f53", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4864, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004864__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4865-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4865, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4866, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5187", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4866", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a59b05b6-6872-42b0-901f-9db229141aec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4865, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004865__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4866-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4866, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4867, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5188", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4867", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "764f5ab0-0c1c-4b46-947b-140ccc2b56bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4866, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004866__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4867-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4867, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4868, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5189", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4868", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d568ecdc-fa3d-4807-bc58-f51dca979422", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4867, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004867__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4868-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4868, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4869, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5190", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4869", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74092346-a320-4be9-965a-e22a2e3799ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4868, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004868__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4869-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4869, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4870, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5191", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4870", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01bc5a49-6cab-453f-a412-ba7c670b91ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4869, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004869__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4870-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4870, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4871, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5192", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4871", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "231fe0ee-d759-4793-a762-2396deeed853", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4870, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004870__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4871-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4871, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4872, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5193", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4872", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "607b1f81-1d3f-4dea-b9de-01c5c27c1525", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4871, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004871__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4872-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4872, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4873, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5194", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4873", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9eeab5a-3677-495c-bb7d-5135fb36656b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4872, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004872__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4873-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4873, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4874, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5195", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4874", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c797ff3-f9a4-4e55-ac83-1ce7fd5ff67b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 422986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4873, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004873__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4874-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4874, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4875, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5196", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4875", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "347a650c-2d08-458f-8b7f-87cb230ec4e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4874, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004874__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4875-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4875, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "422937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4876, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5197", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4876", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18320e5e-7b10-4344-9412-f14470790b2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4875, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004875__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4876-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4876, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4877, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5198", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4877", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8fdc1dbe-2c60-4306-94c0-4067eef1c68c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4876, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004876__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4877-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4877, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4878, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5199", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4878", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "babfe698-ff78-4cf5-b84e-681197bc3899", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4877, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004877__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4878-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4878, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4879, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5200", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4879", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33f4c1a3-0e94-4bae-ab99-44c8393c01d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4878, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004878__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4879-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4879, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4880, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5201", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4880", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41c04905-16eb-4367-bb1d-5447081abb62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4879, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004879__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4880-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4880, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4881, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5202", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4881", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0759013d-5902-4034-a441-bc49a54da961", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4880, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004880__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4881-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4881, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4882, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5203", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4882", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c53b275-1ecb-4002-8300-a36bafdb7cd2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4881, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004881__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4882-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4882, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4883, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5204", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4883", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa81a859-c167-40bb-b0f5-d48eac1fbb4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4882, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004882__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4883-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4883, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4884, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5205", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4884", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1d3723b-52ed-4378-ad87-e346bebf5e28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4883, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004883__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4884-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4884, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4885, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5206", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4885", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13b44b8e-d166-4f53-ac08-50a8e4e30174", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 423940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4884, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004884__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4885-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4885, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4886, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5207", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4886", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "947135bb-d5de-4b4a-983e-6f75a3e3f7da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4885, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004885__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4886-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4886, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4887, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5208", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4887", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c12e090-6938-4ebd-a284-1c5ba7f788cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4886, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004886__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4887-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4887, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "423984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4888, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5209", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4888", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3cb1bc7-913b-4018-8abf-e6e627a78b34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4887, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004887__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4888-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4888, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4889, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5210", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4889", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2fad34b2-b42e-4e22-8faf-58d0cfd1e647", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4888, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004888__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4889-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4889, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4890, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5211", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4890", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98b48437-2513-44e2-b5db-4010622636a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4889, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004889__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4890-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4890, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4891, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5212", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4891", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96f982c8-a58d-45dd-a41e-d051d5592022", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4890, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004890__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4891-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4891, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4892, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5213", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4892", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e92d63be-d700-4552-aa98-25a64769e686", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4891, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004891__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4892-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4892, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4893, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5214", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4893", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf4116f1-9e0f-4d7f-825d-d8f8f253c20c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4892, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004892__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4893-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4893, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4894, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5215", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4894", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d13380b5-60c0-4b24-94ac-23a41d9e0834", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4893, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004893__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4894-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4894, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4895, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5216", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4895", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee64aa8f-1ccf-417d-aa1e-7c6e136dd839", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4894, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004894__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4895-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4895, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4896, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5217", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4896", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a752b193-100b-4ffc-9d51-b2a01fa312bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4895, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004895__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4896-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4896, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4897, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5218", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4897", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42570837-5723-41fd-b2a7-f55a236a5cb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 424986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4896, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004896__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4897-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4897, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4898, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5219", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4898", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e233d08-9959-4f0f-94f5-a4604281f5cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4897, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004897__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4898-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4898, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "424937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4899, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5220", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4899", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b01b1ed-5a82-4068-b93e-bfa343612a92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4898, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004898__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4899-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4899, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4900, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5221", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4900", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f968aa5-71c2-4311-b6a4-d2656aa48115", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4899, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004899__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4900-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4900, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4901, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5222", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4901", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf67de4f-39f4-4091-89e7-db200d5a79e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4900, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004900__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4901-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4901, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4902, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5223", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4902", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85aa86aa-f950-4108-8ea1-23b6d87c605f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4901, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004901__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4902-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4902, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4903, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5224", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4903", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "edb626b9-e211-44fb-8d20-0781ef62b7f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4902, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004902__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4903-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4903, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4904, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5225", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4904", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "002867ab-74b8-4f57-aa6e-779da36dc938", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4903, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004903__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4904-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4904, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4905, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5226", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4905", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2b574c0-4ae6-4159-812e-38bcf6dbc3ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4904, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004904__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4905-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4905, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4906, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5227", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4906", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d8b0d58-dd05-473f-bc74-f5ae02d07e1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4905, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004905__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4906-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4906, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4907, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5228", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4907", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcb62749-e4c4-4c57-b911-7b7ae7f33ca9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4906, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004906__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4907-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4907, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4908, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5229", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4908", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7caa446c-84a5-450f-bbe1-93ed2e516a6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 425940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4907, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004907__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4908-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4908, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4909, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5230", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4909", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd2a9886-0ec4-4ff8-9779-cc896dd3ab1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4908, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004908__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4909-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4909, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4910, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5231", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4910", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18a27bab-3f2f-43bb-b6ca-799aec081b9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4909, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004909__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4910-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4910, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "425968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4911, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5232", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4911", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "894be481-611b-4589-a066-0ec9c9139a5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4910, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004910__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4911-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4911, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4912, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5233", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4912", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d6e45de-7c11-4173-b8b8-614c8f985970", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4911, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004911__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4912-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4912, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4913, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5234", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4913", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b553a496-952a-4c83-91f1-3209214cd78e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4912, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004912__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4913-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4913, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4914, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5235", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4914", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a667abac-1a41-4e75-9501-d886705b135f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4913, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004913__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4914-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4914, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4915, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5236", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4915", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6013506c-284b-42b6-8905-748d9650a04c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4914, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004914__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4915-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4915, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4916, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5237", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4916", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9173a69f-1626-4c70-af4d-42e1b3a37da4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4915, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004915__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4916-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4916, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4917, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5238", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4917", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7d6694e-7d1c-4596-a7a4-f459c75e349c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4916, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004916__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4917-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4917, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4918, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5239", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4918", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a2b5ceb-fe06-4b5b-aeb0-03e2f90ad270", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4917, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004917__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4918-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4918, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4919, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5240", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4919", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9bd9600-1fcf-43c0-b0fe-2ae3de8a7561", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4918, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004918__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4919-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4919, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4920, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5241", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4920", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ed6d1db-72e7-4041-a981-345ac2181813", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 426971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4919, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004919__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4920-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4920, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4921, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5242", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4921", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "553a9c8d-4216-414e-8998-e03923df5e15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4920, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004920__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4921-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4921, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "426921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4922, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5243", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4922", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b65a3d16-89db-4138-9ccc-41cca666e395", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4921, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004921__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4922-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4922, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4923, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5244", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4923", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8bccff83-7951-4294-ba4b-5b3ea4c77ae5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4922, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004922__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4923-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4923, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4924, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5245", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4924", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebf6329b-c086-47aa-b2ff-930f5600fccd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4923, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004923__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4924-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4924, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4925, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5246", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4925", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80da25e1-f053-4d32-baae-af994870b44f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4924, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004924__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4925-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4925, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4926, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5247", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4926", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "705a8ab6-32d7-43ea-b624-13467e6ead39", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4925, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004925__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4926-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4926, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4927, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5248", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4927", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21884414-6506-46ba-a00e-8e04895e2f1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4926, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004926__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4927-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4927, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4928, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5249", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4928", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b712ba82-74fa-4037-b897-9b3c093e0b58", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4927, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004927__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4928-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4928, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4929, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5250", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4929", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7785f96a-c68b-4b9a-b1dd-f53c51d56877", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4928, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004928__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4929-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4929, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4930, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5251", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4930", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8cf688a-f538-49f3-8960-345fb25cb3a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4929, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004929__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4930-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4930, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4931, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5252", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4931", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c37a9797-de42-460b-aef9-0b8259b3755f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 427924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4930, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004930__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4931-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4931, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4932, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5253", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4932", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a80ad1fa-9835-48da-91da-80e767d348ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4931, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004931__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4932-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4932, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4933, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5254", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4933", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "321823ca-a5bb-4588-bf83-aa7423be5ec9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4932, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004932__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4933-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4933, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "427968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4934, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5255", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4934", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4f16094-8f04-4499-b227-e0f0cbfb181d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4933, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004933__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4934-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4934, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4935, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5256", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4935", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b7dd558-f073-4e3e-b740-03152682b521", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4934, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004934__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4935-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4935, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4936, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5257", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4936", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1807df97-6ae4-49cd-83bf-29843ec5d0d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4935, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004935__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4936-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4936, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4937, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5258", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4937", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03bfba8e-2574-4c83-ba13-efa42aa22857", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4936, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004936__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4937-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4937, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4938, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5259", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4938", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b8417d4-5e40-4123-88b8-ab0aaeb4116e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4937, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004937__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4938-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4938, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4939, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5260", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4939", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2abb5fba-65e1-4b2f-8ddb-86e1225419da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4938, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004938__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4939-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4939, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4940, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5261", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4940", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0db8ff8-3f57-4cd7-86f2-5a5262e334a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4939, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004939__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4940-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4940, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4941, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5262", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4941", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61728755-2e4a-4224-b2a0-13be4f22d01f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4940, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004940__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4941-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4941, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4942, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5263", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4942", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d04d0545-2b32-426e-b21f-3d3becc0f9be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4941, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004941__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4942-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4942, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4943, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5264", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4943", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0349d54-965a-4a45-bb9d-79e42f5b7aff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 428971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4942, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004942__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4943-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4943, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4944, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5265", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4944", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "750c1f0c-9062-4950-808a-d933241ea743", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4943, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004943__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4944-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4944, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "428921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4945, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5266", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4945", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64e8bb71-3e59-4ea0-8be2-d445371034e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4944, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004944__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4945-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4945, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4946, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5267", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4946", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c6ba2b6-8adf-4b54-8ef9-38a5cd43a244", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4945, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004945__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4946-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4946, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4947, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5268", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4947", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2443852c-eba1-4d14-a2c9-f083377704fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4946, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004946__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4947-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4947, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4948, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5269", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4948", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fbe2464c-9cae-4411-b540-b0d368f95e27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4947, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004947__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4948-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4948, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4949, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5270", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4949", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2501eb59-626b-4337-b6ab-dc366b1c4bd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4948, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004948__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4949-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4949, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4950, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5271", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4950", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3b70650-180d-42d6-a614-eff57c46cec1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4949, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004949__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4950-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4950, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4951, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5272", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4951", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52ce5a65-89b7-4bc9-a220-85700458b0a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4950, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004950__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4951-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4951, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4952, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5273", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4952", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db27df52-7e1b-49fc-9f67-59124146f753", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4951, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004951__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4952-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4952, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4953, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5274", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4953", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0b2dde7-dfa2-4adf-9292-80243db850d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4952, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004952__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4953-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4953, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4954, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5275", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4954", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "463fb152-3d01-4dce-b08b-a2970bb9f974", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 429924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4953, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004953__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4954-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4954, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4955, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5276", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4955", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72ae07b2-c759-4716-9b8f-f6ff1fab206f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4954, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004954__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4955-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4955, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4956, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5277", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4956", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b1515c2-bb1b-4bd4-bb24-35fee177e1b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4955, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004955__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4956-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4956, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "429968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4957, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5278", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4957", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c6fd620-5523-465e-b6c9-351a9a3dd25d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4956, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004956__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4957-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4957, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4958, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5279", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4958", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e63a4973-1686-4aa5-adff-4945d4a1d9a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4957, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004957__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4958-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4958, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4959, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5280", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4959", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b5e324f-4149-4383-b5d4-2de557eead23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4958, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004958__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4959-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4959, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4960, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5281", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4960", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7a8ee64-0197-4d9a-8b87-11b4049ce558", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4959, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004959__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4960-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4960, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4961, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5282", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4961", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbb75d5e-9030-48be-a29f-eb5741d87f1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4960, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004960__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4961-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4961, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4962, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5283", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4962", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bdcbb78-a198-4c67-8781-5030e18baac6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4961, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004961__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4962-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4962, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4963, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5284", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4963", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c66bcfa-9bdb-4b68-9206-c5eb7e8ca986", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4962, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004962__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4963-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4963, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4964, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5285", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4964", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f0e2133-9cd6-473b-9bf3-1cae85b211b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4963, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004963__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4964-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4964, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4965, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5286", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4965", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a273310-f31b-4add-b340-d7de76f7760f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4964, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004964__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4965-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4965, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4966, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5287", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4966", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6035f71-a48b-4277-838a-8e8d483a596e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 430971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4965, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004965__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4966-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4966, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4967, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5288", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4967", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9f5f0dc-d41a-4b7c-9cd1-ecd5262ff800", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4966, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004966__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4967-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4967, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "430921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4968, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5289", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4968", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7cd19574-9aae-4dce-be14-71ac311c49ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4967, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004967__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4968-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4968, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4969, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5290", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4969", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "808be9dc-4c39-481c-a3ad-e7d04b703037", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4968, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004968__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4969-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4969, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4970, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5291", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4970", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "510f5c50-0431-4f6b-8df3-8e8965c33b33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4969, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004969__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4970-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4970, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4971, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5292", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4971", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eabe16a7-6902-43a2-9155-e65e2e3ddd8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4970, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004970__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4971-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4971, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4972, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5293", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4972", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe10e619-47a3-4f31-8f45-9e0e29a63da7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4971, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004971__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4972-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4972, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4973, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5294", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4973", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "760d2466-db27-4192-a6d8-d8a62760b93d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4972, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004972__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4973-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4973, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4974, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5295", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4974", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "281aeb5e-f85f-42b0-8e1c-4fbef8c6a175", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4973, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004973__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4974-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4974, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4975, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5296", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4975", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b31998c7-d3df-40b1-a701-27bf8883cc13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4974, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004974__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4975-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4975, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4976, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5297", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4976", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b346a1fc-817f-46c9-a294-ae7e42c155b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4975, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004975__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4976-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4976, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4977, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5298", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4977", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62b0c028-1422-4f0f-ad3e-ca40551a1c95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 431924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4976, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004976__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4977-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4977, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4978, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5299", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4978", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccd0503e-4277-473f-a1b0-8d4fc2dc2823", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4977, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004977__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4978-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4978, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4979, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5300", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4979", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1316e89c-27a1-4d65-bad7-45abc76dc636", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4978, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004978__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4979-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4979, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "431953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4980, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5301", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4980", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c7a43df-137f-4d17-b5be-9ecf5356eb15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4979, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004979__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4980-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4980, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4981, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5302", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4981", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d80876f1-a4ca-478a-b1f0-02338d15f71d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4980, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004980__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4981-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4981, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4982, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5303", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4982", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8734bc8d-f4eb-4d30-aa9b-e81d9f3de500", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4981, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004981__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4982-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4982, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4983, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5304", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4983", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "372f2c84-3ee7-402b-9640-4f463391cb44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4982, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004982__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4983-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4983, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4984, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5305", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4984", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f510a1ad-2197-41a3-9c0d-9084b12ec4d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4983, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004983__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4984-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4984, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4985, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5306", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4985", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9bb7a1f0-aec1-44b4-9cab-ea84c3552b2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4984, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004984__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4985-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4985, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4986, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5307", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4986", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46dd4e10-b59b-4f48-9715-8509b6ff7faf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4985, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004985__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4986-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4986, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4987, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5308", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4987", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87a96a0c-45f7-467c-8cab-104d82a5f05e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4986, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004986__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4987-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4987, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4988, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5309", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4988", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cbd85652-9930-47ba-b843-24de81be33bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4987, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004987__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4988-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4988, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4989, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5310", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4989", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5800e5b-28e0-4f12-a67f-01582ef9246a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 432955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4988, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004988__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4989-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4989, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4990, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5311", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4990", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85bdf974-8312-4bd9-b996-b10b1363a6e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4989, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004989__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4990-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4990, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "432906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4991, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5312", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4991", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42037ed2-5a2d-46ac-bda6-c32fb5cf6d72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4990, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004990__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4991-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4991, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4992, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5313", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4992", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fcce2b3-9e8d-4792-937c-910a9f0213b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4991, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004991__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4992-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4992, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4993, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5314", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4993", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "823e6146-1131-47ac-b585-b6566d2f372f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4992, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004992__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4993-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4993, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4994, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5315", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4994", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "053233d5-7435-4ecc-b4b5-38e33640aace", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4993, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004993__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4994-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4994, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4995, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5316", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4995", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d162347c-4d47-406c-9be2-8034672937d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4994, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004994__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4995-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4995, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4996, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5317", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4996", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "934bbc1f-83d4-4349-8a5a-784c079cecb3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4995, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004995__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4996-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4996, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4997, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5318", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4997", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b85b5e91-3a5d-43c2-a789-3805772bd4ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4996, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004996__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4997-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4997, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4998, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5319", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4998", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f13c61ba-7b47-4182-bf0f-93d698636200", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4997, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004997__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4998-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4998, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 4999, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5320", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "4999", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a589ce28-6d35-4047-bf6f-c24aee07f02d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4998, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004998__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-4999-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 4999, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5000, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5321", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5000", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "365a7507-672e-4ff8-8825-d08a66c395d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 433908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 4999, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000004999__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5000-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5000, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5001, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5322", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5001", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d7fc84e-583c-4c38-948b-b7a51c4c11c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5000, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5001-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5001, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5002, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5323", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5002", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bee5f7be-77c1-437b-b790-2e3d882613ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5001, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005001__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5002-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5002, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "433953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5003, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5324", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5003", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc8e859b-150c-4e53-8171-9c91446f23b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5002, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005002__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5003-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5003, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5004, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:25:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5325", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5004", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76016d02-be97-44e0-8beb-4cf9dcb5bf8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5003, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005003__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5004-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5004, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5005, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5326", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5005", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3123a6a-37d3-4b54-9419-b8c004b7b278", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5004, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005004__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5005-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5005, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5006, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5327", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5006", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0703b615-b7fa-4fec-a4a4-dba1f699e694", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5005, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005005__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5006-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5006, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5007, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5328", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5007", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c84954ae-d711-423e-be73-c1aa0acf9e14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5006, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005006__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5007-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5007, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5008, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5329", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5008", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c03f2ad4-77cf-4a92-94ce-991a2463aef5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5007, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005007__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5008-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5008, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5009, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5330", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5009", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a8704fd7-3168-4b18-830c-65c485aa470c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5008, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005008__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5009-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5009, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5010, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5331", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5010", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32e68423-7371-40b3-bd04-5d818cbb42e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5009, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005009__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5010-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5010, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5011, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5332", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5011", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdc90eea-3ac9-421c-b447-ae7b54e9f4ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5010, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005010__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5011-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5011, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5012, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5333", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5012", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaaad5e7-2ba3-452b-b2d8-13cfab234773", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 434955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5011, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005011__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5012-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5012, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5013, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5334", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5013", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fe9c649-3824-4901-a562-f87e0ba181cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5012, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005012__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5013-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5013, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "434906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5014, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5335", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5014", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1987c51-cf49-410d-b2f3-cb20b8a76abb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5013, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005013__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5014-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5014, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5015, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5336", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5015", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "621c23eb-8bf3-463d-b36b-8f9daca1197c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5014, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005014__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5015-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5015, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5016, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5337", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5016", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ea049c5-c1c9-49ce-98bc-8ab576c93998", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5015, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005015__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5016-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5016, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5017, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5338", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5017", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6c074f1-ead8-44d4-ac12-8f6b78ab212e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5016, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005016__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5017-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5017, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5018, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5339", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5018", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "946e7f6d-25b4-4a4a-8f42-ca63744ecb92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5017, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005017__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5018-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5018, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5019, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5340", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5019", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55aa1b3b-f87e-4fa5-9f52-a09b2838acc3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5018, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005018__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5019-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5019, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5020, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5341", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5020", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e0cf8c7-f2e1-44ab-9794-d5e3d9cbbcba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5019, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005019__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5020-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5020, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5021, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5342", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5021", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c81d38a3-c158-42e1-81bc-c22d5d443601", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5020, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005020__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5021-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5021, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5022, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5343", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5022", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b37c4143-71b8-4489-a3e8-2d1ff5b06863", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5021, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005021__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5022-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5022, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5023, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5344", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5023", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfb7b561-a6ee-4620-ac48-d34c21e08529", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 435908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5022, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005022__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5023-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5023, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5024, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5345", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5024", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f041439c-4f2a-4cef-87d7-ba253c21f722", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5023, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005023__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5024-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5024, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5025, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5346", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5025", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "718cd35a-16c1-4646-b001-fe6e82a7fef7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5024, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005024__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5025-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5025, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "435953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5026, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5347", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5026", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c3378d6-7ca3-4dce-8758-1180122bbfe8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5025, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005025__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5026-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5026, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5027, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5348", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5027", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9237dc9-7ee9-4ba9-afcf-6a1f33e692ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5026, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005026__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5027-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5027, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5028, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5349", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5028", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f77fba0-734d-4258-984b-9be23ac2e79e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5027, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005027__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5028-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5028, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5029, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5350", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5029", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d48d3a2-7a10-495d-b33c-0db8bf46b523", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5028, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005028__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5029-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5029, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5030, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5351", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5030", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e2cdaee-107a-467e-927d-81ebdfce054a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5029, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005029__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5030-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5030, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5031, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5352", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5031", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e16c4b87-8785-4e1c-b6b4-15b155f42362", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5030, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005030__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5031-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5031, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5032, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5353", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5032", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0d1cd54-ef72-4d35-a6ef-84334caa71e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5031, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005031__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5032-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5032, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5033, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5354", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5033", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcd267e4-41f9-45cd-8ab5-6fa5bd633ff8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5032, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005032__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5033-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5033, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5034, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5355", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5034", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "638f82a3-029f-4b4d-b42f-16b0e2921d1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5033, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005033__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5034-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5034, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5035, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5356", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5035", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89441458-a36d-4489-88a9-add017b106ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 436955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5034, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005034__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5035-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5035, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5036, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5357", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5036", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be86bc3c-94ee-4d97-818c-8f9b0149c027", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5035, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005035__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5036-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5036, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5037, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5358", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5037", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d728927b-afb4-4649-9bc0-a984ee489e0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5036, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005036__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5037-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5037, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "436984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5038, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5359", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5038", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a75ad94-2233-4a90-b734-31ce82d8ae4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5037, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005037__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5038-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5038, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5039, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5360", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5039", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9067ba1b-4119-4e08-a699-d2302921ae01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5038, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005038__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5039-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5039, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5040, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5361", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5040", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7edbc4d-31a7-4b6a-bbb8-675ec62d8250", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5039, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005039__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5040-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5040, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5041, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5362", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5041", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "868c3b53-6411-4680-8e94-466eddcb6d3c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5040, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005040__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5041-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5041, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5042, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5363", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5042", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e602e3f0-de56-44bd-b6da-8b044ecd8d1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5041, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005041__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5042-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5042, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5043, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5364", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5043", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c349c139-9274-42ed-ba79-f3f76b69098b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5042, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005042__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5043-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5043, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5044, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5365", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5044", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89749398-fbbe-4b37-8eda-1aabed883747", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5043, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005043__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5044-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5044, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5045, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5366", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5045", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c18fa7de-0c4d-4d2c-b4ed-992864114151", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5044, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005044__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5045-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5045, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5046, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5367", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5046", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a194071e-4b14-4ae9-ab99-6bcbf9d106df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5045, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005045__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5046-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5046, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5047, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5368", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5047", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7bd3f209-39cd-4d00-a9fc-0f3c2feb7700", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 437986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5046, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005046__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5047-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5047, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5048, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5369", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5048", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8971111f-1ee9-4d4c-971e-a881481e6dee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5047, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005047__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5048-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5048, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "437937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5049, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5370", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5049", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbebf73f-ed29-467e-adea-8a2eb42185fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5048, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005048__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5049-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5049, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5050, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5371", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5050", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de2af1e9-9eb5-4bd4-b6e3-25a854b04bf4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5049, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005049__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5050-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5050, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5051, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5372", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5051", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75863797-6d14-4d34-8dac-1b4e16000701", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5050, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005050__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5051-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5051, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5052, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5373", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5052", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aef503b2-7f71-4fe1-a534-f2cabf2ae3ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5051, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005051__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5052-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5052, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5053, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5374", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5053", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d248ed14-2d25-45aa-b035-5b227f5ae24c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5052, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005052__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5053-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5053, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5054, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5375", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5054", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33cf1c4b-c475-4f06-8887-06d165e631fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5053, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005053__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5054-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5054, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5055, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5376", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5055", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74f563c2-b5c8-4e26-a987-b05946b48ebb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5054, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005054__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5055-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5055, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5056, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5377", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5056", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f2baee0-6d33-4490-84ad-f78574c3aaa4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5055, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005055__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5056-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5056, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5057, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5378", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5057", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ca88a72-9112-48ce-bade-3f0680475791", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5056, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005056__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5057-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5057, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5058, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5379", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5058", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8296d708-d419-4ec6-a6aa-dd638479f93a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 438940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5057, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005057__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5058-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5058, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5059, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5380", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5059", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca78be6a-0842-40b9-8b3d-5f767f127922", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5058, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005058__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5059-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5059, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5060, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5381", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5060", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6ab9488-16f4-49ad-9746-12c4b8886268", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5059, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005059__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5060-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5060, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "438984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5061, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5382", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5061", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "caadab61-6118-4038-ba47-35710e3a2405", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5060, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005060__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5061-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5061, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5062, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5383", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5062", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69c768f5-cf43-4924-8b36-757c71ee846a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5061, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005061__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5062-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5062, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5063, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5384", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5063", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "163f9dfb-f8e4-448d-9710-84ef77e7f58f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5062, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005062__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5063-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5063, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5064, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5385", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5064", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "683cf30f-cbaa-4b9b-8d43-7b3d4abc50b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5063, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005063__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5064-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5064, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5065, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5386", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5065", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4760e52d-ad99-4582-be63-63da8e2590e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5064, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005064__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5065-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5065, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5066, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5387", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5066", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "446d85e5-0375-4048-a597-3346436c0e19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5065, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005065__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5066-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5066, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5067, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5388", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5067", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b7f9aff-bcbe-47f4-a0f0-3b53555f206c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5066, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005066__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5067-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5067, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5068, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5389", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5068", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be2461ce-206c-4873-8f9e-ae7818c16b37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5067, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005067__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5068-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5068, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5069, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5390", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5069", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70ceb20f-7318-40df-9dad-f5969ee8f031", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5068, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005068__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5069-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5069, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5070, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5391", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5070", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84b6db25-c936-42a9-9a49-085cce71104a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 439986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5069, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005069__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5070-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5070, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5071, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5392", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5071", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75d09d7a-513c-44ef-b6ce-ee0f9440ac82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5070, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005070__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5071-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5071, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "439937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5072, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5393", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5072", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8fea8c35-2019-4980-b61b-27b4b1a2a4ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5071, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005071__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5072-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5072, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5073, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5394", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5073", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cad07e44-7a90-4ec8-aea7-8c7b2f268011", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5072, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005072__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5073-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5073, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5074, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5395", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5074", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "254539b3-a9b3-422f-9d69-472edcb43519", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5073, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005073__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5074-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5074, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5075, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5396", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5075", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eaf0b076-0890-4b06-b172-cf0606230618", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5074, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005074__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5075-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5075, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5076, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5397", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5076", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "727b3895-a3d5-4629-941e-71e0b501d9f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5075, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005075__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5076-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5076, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5077, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5398", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5077", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2a810ab-279b-45c4-b7c2-5338e2bcf23b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5076, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005076__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5077-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5077, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5078, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5399", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5078", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4285dedc-598b-4c26-ab5c-878478642359", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5077, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005077__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5078-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5078, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5079, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5400", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5079", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b872786d-9ac5-444e-afae-d04ae4f87840", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5078, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005078__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5079-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5079, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5080, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5401", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5080", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcf2123b-8b57-404a-874f-bbc1c12c411e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5079, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005079__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5080-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5080, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5081, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5402", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5081", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baf000c4-c0d5-485b-99f9-34fe09a5aec9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 440940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5080, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005080__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5081-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5081, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5082, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5403", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5082", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d5523cb-b934-4b29-b0e7-7bf8021f6db9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5081, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005081__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5082-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5082, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5083, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5404", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5083", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "480d0b01-e382-475c-aa84-f662831a41e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5082, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005082__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5083-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5083, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "440984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5084, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5405", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5084", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd83f820-47db-4b0d-82ac-22aebe1d28d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5083, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005083__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5084-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5084, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5085, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5406", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5085", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7da8d2d2-b3f7-4a99-ac46-5d7a9bb39186", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5084, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005084__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5085-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5085, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5086, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5407", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5086", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36a9d622-507a-4e4d-ad20-4177ea558a7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5085, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005085__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5086-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5086, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5087, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5408", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5087", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3aaad8c1-541e-4502-ba47-e48b30bb9ad6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5086, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005086__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5087-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5087, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5088, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5409", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5088", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fb3acd4-529e-42af-b6d0-3dfad900bc93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5087, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005087__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5088-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5088, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5089, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5410", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5089", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43c1e0ca-cf7a-4c7a-aa7f-17c012fcded6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5088, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005088__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5089-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5089, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5090, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5411", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5090", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e7773d5-235c-42f4-8ca8-c60e79a72301", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5089, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005089__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5090-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5090, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5091, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5412", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5091", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "573c2229-8b17-4c47-9114-3ef194efec09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5090, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005090__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5091-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5091, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5092, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5413", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5092", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc11b39d-5c67-4fad-9d96-c1bdc79e5e1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5091, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005091__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5092-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5092, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5093, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5414", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5093", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d72d7b9-b357-400f-be38-8c2d394f34b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 441986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5092, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005092__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5093-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5093, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5094, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5415", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5094", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19496807-dcd4-4f55-8542-bebc0578e5c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5093, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005093__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5094-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5094, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "441937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5095, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5416", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5095", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e7968b8-96f9-44ac-9095-3a08f918b3ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5094, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005094__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5095-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5095, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5096, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5417", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5096", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6b895d8-c9cf-411e-b82a-79e7bfcbdc5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5095, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005095__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5096-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5096, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5097, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5418", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5097", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1b64d0e-58a9-4386-b339-8e238b0b5ec3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5096, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005096__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5097-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5097, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5098, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5419", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5098", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3908ef45-b073-49bf-9633-95fbc1c42052", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5097, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005097__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5098-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5098, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5099, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5420", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5099", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4183013-a8b7-4d58-a6d1-f5955e956233", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5098, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005098__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5099-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5099, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5100, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5421", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5100", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77964948-7fc9-44c7-a41d-6c29c13684ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5099, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005099__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5100-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5100, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5101, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5422", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5101", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16f64343-3ab9-4824-8b0a-c62ccb6ad2a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5100, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005100__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5101-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5101, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5102, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5423", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5102", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e2a534e-96e6-4944-b7a1-ccd8343f750e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5101, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005101__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5102-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5102, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5103, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5424", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5103", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbb76409-877e-47c8-9643-4eb179f86942", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5102, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005102__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5103-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5103, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5104, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5425", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5104", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b6ddd37-f652-485a-9332-39a0daaf97e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 442940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5103, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005103__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5104-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5104, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5105, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5426", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5105", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e522a1f-561e-46e0-843f-f6662a4170b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5104, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005104__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5105-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5105, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5106, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5427", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5106", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae784069-321e-45cb-8d3d-c4eaf9e42fbe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5105, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005105__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5106-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5106, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "442968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5107, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5428", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5107", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d6e4458-2903-4967-a1fe-ff6758afe2f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5106, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005106__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5107-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5107, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5108, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5429", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5108", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1db2f884-11f0-4d80-8dcf-5dfdc2338790", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5107, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005107__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5108-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5108, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5109, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5430", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5109", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32b4c973-07f0-4adf-90d5-d5c5a1fdde74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5108, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005108__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5109-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5109, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5110, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5431", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5110", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53516245-390b-48d2-9d19-1aa384da1f12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5109, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005109__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5110-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5110, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5111, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5432", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5111", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b215a0f5-c351-483e-851f-b24a2d8bfce4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5110, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005110__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5111-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5111, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5112, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5433", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5112", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3aeee5fb-b8a9-4dd2-a1ab-d58ef63dce4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5111, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005111__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5112-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5112, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5113, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5434", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5113", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce409921-5eb4-4a57-be85-63ae2fb8fa41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5112, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005112__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5113-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5113, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5114, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5435", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5114", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11b0aee6-d3b4-4f2d-8809-a09b8689c298", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5113, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005113__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5114-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5114, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5115, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5436", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5115", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "679e665a-97d2-40ec-9360-f5a6f5ef84ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5114, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005114__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5115-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5115, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5116, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5437", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5116", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c43bc9d0-ec47-4cdb-9ceb-33e6062cb598", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 443971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5115, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005115__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5116-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5116, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5117, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5438", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5117", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1225657-ddb4-4960-a0f9-6a6967e1a45d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5116, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005116__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5117-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5117, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "443921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5118, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5439", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5118", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f944e211-5da2-4a4a-a198-261e779e7d8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5117, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005117__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5118-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5118, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5119, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5440", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5119", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d592221-3607-49d5-aeb5-152a67073be0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5118, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005118__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5119-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5119, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5120, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5441", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5120", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81fcc812-1621-406c-bae3-9b8da4e8b4f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5119, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005119__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5120-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5120, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5121, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5442", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5121", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "daed3294-c108-414a-ae9b-ccb5ff29e29b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5120, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005120__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5121-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5121, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5122, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5443", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5122", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07ded876-672a-48e5-a1e2-541f5bdee69e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5121, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005121__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5122-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5122, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5123, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5444", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5123", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "626744cf-8b69-4826-abc2-34ee4780d95f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5122, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005122__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5123-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5123, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5124, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5445", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5124", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34e05e9e-a255-43f6-a92f-10f8c63148d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5123, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005123__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5124-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5124, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5125, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5446", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5125", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ef65636-8b51-45f1-80ad-59998f1558a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5124, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005124__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5125-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5125, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5126, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5447", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5126", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c209150-8542-4a4a-9716-242c6bbd7dcb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5125, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005125__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5126-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5126, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5127, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5448", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5127", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a256fa1-64b5-4665-aeab-c8946eadb891", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 444924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5126, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005126__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5127-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5127, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5128, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5449", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5128", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3065a6b9-9240-42eb-b888-c0dbf0080e05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5127, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005127__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5128-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5128, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5129, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5450", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5129", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47727bb0-dcfa-4ba5-84f0-fa33d92c6f7b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5128, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005128__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5129-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5129, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "444968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5130, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5451", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5130", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f64ab7a3-0dd3-4bb8-a35f-48c1df1d0140", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5129, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005129__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5130-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5130, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5131, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5452", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5131", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42f0f6a8-5e0c-47d9-ba0f-125bb8fc4db6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5130, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005130__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5131-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5131, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5132, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5453", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5132", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e617b415-8fc0-498e-8e9a-408146fbe728", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5131, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005131__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5132-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5132, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5133, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5454", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5133", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "023aa76b-10dd-48b7-90cb-a5c8906beb02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5132, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005132__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5133-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5133, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5134, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5455", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5134", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3030506-d5d1-4931-857c-7a706ff229f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5133, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005133__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5134-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5134, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5135, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5456", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5135", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47f2fc9f-846a-4d00-a733-e0a679382867", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5134, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005134__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5135-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5135, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5136, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5457", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5136", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02ac9674-a718-4d48-b1a7-4854e13ee9fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5135, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005135__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5136-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5136, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5137, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5458", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5137", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d672e7e0-6e6b-4513-a4d1-f3f3aee73688", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5136, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005136__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5137-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5137, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5138, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5459", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5138", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f6fe100-699f-412c-9bc8-659523b86df8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5137, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005137__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5138-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5138, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5139, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5460", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5139", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3576675a-e0ef-4c78-a924-58bb40ebb941", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 445971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5138, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005138__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5139-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5139, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5140, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5461", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5140", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "989ba594-c2a8-4058-8872-bef7182557cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5139, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005139__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5140-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5140, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "445921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5141, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5462", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5141", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d67e5c57-54af-42cf-a6cb-698d1d62dbce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5140, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005140__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5141-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5141, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5142, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5463", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5142", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "224fe6e8-0b2f-414f-aca0-2e610898e073", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5141, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005141__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5142-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5142, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5143, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5464", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5143", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70805ead-de90-4e20-be55-00af73fde0e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5142, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005142__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5143-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5143, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5144, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5465", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5144", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56cd7505-85d0-40de-b85d-3b5e436557bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5143, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005143__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5144-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5144, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5145, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5466", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5145", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aeb6c1e4-cefb-40c5-8951-c161f701a091", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5144, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005144__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5145-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5145, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5146, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5467", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5146", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59855c68-9ffc-4b1a-a299-854dacf254d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5145, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005145__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5146-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5146, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5147, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5468", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5147", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83f9710b-45d9-49d0-b28e-a5bc1b4d79bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5146, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005146__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5147-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5147, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5148, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5469", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5148", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ade380be-7c86-4280-9439-4946a1c092f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5147, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005147__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5148-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5148, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5149, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5470", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5149", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10b0b02d-b0d1-45dc-a80a-9cd4054f5685", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5148, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005148__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5149-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5149, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5150, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5471", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5150", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f28a8c77-bdcc-455c-8bef-2b33ed8d21d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 446924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5149, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005149__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5150-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5150, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5151, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5472", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5151", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd6822c7-b3e9-4c33-9dc4-5319e8ff246c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5150, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005150__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5151-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5151, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5152, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5473", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5152", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd6447b9-13f8-4b1f-91e4-e24bbbb285d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5151, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005151__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5152-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5152, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "446968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5153, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5474", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5153", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d62c48b3-3dd5-4f84-b137-8711e5291a20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5152, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005152__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5153-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5153, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5154, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5475", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5154", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "329a3dc7-894d-48da-bd7c-7d2b007a65a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5153, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005153__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5154-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5154, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5155, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5476", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5155", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71728f45-8d74-47bd-a772-59a6e75357ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5154, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005154__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5155-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5155, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5156, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5477", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5156", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c53cb4e-d5dd-45ce-ac8e-7065affacf70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5155, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005155__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5156-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5156, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5157, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5478", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5157", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac26a558-da70-4f1c-938f-84acabedbfd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5156, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005156__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5157-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5157, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5158, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5479", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5158", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bd4bbf9-eb02-4b83-b33a-61c7bd38c725", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5157, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005157__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5158-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5158, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5159, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5480", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5159", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7f4c906-8e1f-49be-b8c2-0812e8bd71bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5158, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005158__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5159-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5159, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5160, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5481", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5160", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b04cf096-8dc8-4c3c-a951-5ad000c53f00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5159, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005159__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5160-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5160, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5161, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5482", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5161", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f477e89b-7a70-40bb-b517-15eae0178ed6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5160, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005160__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5161-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5161, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5162, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5483", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5162", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c165689d-902e-4f90-ae9b-914e015c8548", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 447971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5161, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005161__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5162-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5162, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5163, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5484", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5163", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05afc225-33ca-49d2-ba8e-bc2617dafdd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5162, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005162__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5163-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5163, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "447921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5164, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5485", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5164", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56f9e82c-20c1-4d59-a023-ae26ec376485", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5163, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005163__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5164-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5164, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5165, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5486", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5165", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62d0b930-7112-44a6-9871-1a61f6c01757", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5164, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005164__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5165-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5165, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5166, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5487", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5166", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "118cd2d7-7d17-484f-9bcd-f2f9f80df3b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5165, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005165__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5166-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5166, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5167, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5488", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5167", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86cdc1c4-f7b5-4a9d-b291-cf71e38bbba2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5166, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005166__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5167-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5167, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5168, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5489", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5168", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd63d57f-cfd7-47e5-9a52-bcdde5f94dca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5167, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005167__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5168-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5168, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5169, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5490", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5169", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bb4b1e1-1308-47da-ba43-03941a52328a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5168, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005168__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5169-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5169, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5170, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5491", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5170", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4adbda8-08dc-4ad2-9792-6ab987554d05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5169, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005169__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5170-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5170, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5171, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5492", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5171", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90bcf1aa-1d45-45a9-802e-9abaaea0e432", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5170, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005170__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5171-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5171, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5172, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5493", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5172", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87fee7c9-aa42-4d2d-ad04-5ac643f8c2f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5171, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005171__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5172-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5172, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5173, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5494", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5173", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8908574b-fc41-40f4-8836-351e42ba3cf6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 448924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5172, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005172__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5173-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5173, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5174, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5495", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5174", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e035318-db2d-436b-af92-89f5d2f0ac6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5173, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005173__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5174-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5174, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5175, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5496", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5175", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1f40437-8b96-49bd-8920-d0d0fe625ed2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5174, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005174__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5175-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5175, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "448953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5176, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5497", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5176", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40e706fb-5ee6-4157-ab99-5beeddb57b2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5175, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005175__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5176-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5176, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5177, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5498", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5177", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12152dc2-b698-42f1-8638-335962173b4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5176, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005176__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5177-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5177, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5178, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5499", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5178", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "888691da-95b4-480e-908e-ee609ff33727", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5177, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005177__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5178-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5178, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5179, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5500", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5179", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e25977ea-b21b-44ec-995a-0b1f97f9e18b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5178, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005178__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5179-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5179, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5180, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5501", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5180", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fc23d4c-0bf3-48ec-b5e1-43b9e7b07e27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5179, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005179__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5180-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5180, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5181, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5502", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5181", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b857203f-3951-4612-8dd0-1a0f22b6f581", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5180, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005180__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5181-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5181, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5182, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5503", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5182", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e97f078d-e447-4233-87d5-5a77795e5e61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5181, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005181__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5182-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5182, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5183, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5504", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5183", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d5c18e2-751e-4bc1-910c-05d650a584cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5182, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005182__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5183-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5183, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5184, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5505", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5184", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5b1251b-9334-4474-b1be-5e09d3b7dca1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5183, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005183__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5184-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5184, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5185, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5506", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5185", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c45ba29a-9ddc-4d7f-99a1-ca23702ee39a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 449955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5184, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005184__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5185-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5185, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5186, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5507", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5186", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f77c9421-8af2-482e-bc55-304a19e2a5dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5185, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005185__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5186-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5186, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "449906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5187, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5508", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5187", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38062511-a800-4ab9-8d78-ae26a18fcd27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5186, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005186__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5187-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5187, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5188, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5509", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5188", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2989f68d-8836-4bec-bfb0-83e226310550", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5187, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005187__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5188-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5188, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5189, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5510", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5189", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "243b3891-dc05-47ec-b36f-ab3ffb43aee3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5188, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005188__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5189-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5189, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5190, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5511", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5190", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a438abd-b1a1-47d6-9fc6-73a678888701", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5189, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005189__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5190-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5190, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5191, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5512", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5191", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dfee4d0f-b655-49c2-8381-c98f8a09d898", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5190, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005190__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5191-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5191, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5192, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5513", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5192", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12e4855d-9302-411e-b3a1-6244e4be1d36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5191, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005191__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5192-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5192, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5193, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5514", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5193", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e1112b4-587d-420e-b205-e64d77e63d6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5192, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005192__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5193-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5193, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5194, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5515", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5194", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6c860ba-18b6-46ac-8eae-474ced7110eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5193, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005193__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5194-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5194, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5195, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5516", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5195", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc06be83-bf79-4030-8b9e-6fab1f0dfd7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5194, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005194__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5195-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5195, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5196, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5517", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5196", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eef30fed-2f72-4f6f-b1ca-83a3b1dc14d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 450908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5195, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005195__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5196-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5196, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5197, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5518", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5197", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2044f17a-7c0a-4b66-8388-9e521565df37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5196, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005196__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5197-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5197, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5198, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5519", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5198", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a4959f7-025e-42f1-beaf-18872049f3cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5197, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005197__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5198-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5198, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "450953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5199, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5520", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5199", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2380dcd0-9a52-4672-8e3b-7758b4e276fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5198, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005198__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5199-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5199, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5200, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5521", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5200", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "884ec5af-ff7e-46e1-8dcd-c43b2f00bbc8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5199, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005199__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5200-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5200, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5201, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5522", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5201", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f34ce68d-fb64-4117-ab2d-5815e1840bcf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5200, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005200__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5201-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5201, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5202, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5523", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5202", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0daeb9b6-099c-44f3-abc3-028babb5e780", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5201, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005201__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5202-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5202, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5203, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5524", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5203", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e4bfc2a-4c95-458a-adb6-9817ae09aa0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5202, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005202__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5203-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5203, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5204, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5525", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5204", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d881d1de-f4e9-474b-871c-e15985dee2a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5203, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005203__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5204-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5204, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5205, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5526", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5205", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1300a0ea-5c44-4436-bcf3-15e97fc27740", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5204, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005204__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5205-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5205, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5206, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5527", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5206", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "315f520f-0bf0-4354-bb1b-1e46a0ab859e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5205, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005205__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5206-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5206, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5207, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5528", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5207", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac8dc61f-798d-4b9d-976c-ed76ba883d3a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5206, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005206__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5207-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5207, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5208, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5529", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5208", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "067607c1-74d1-4f3f-a57c-d51c91008abb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5207, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005207__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5208-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5208, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5209, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5530", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5209", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db457801-961a-4205-a841-3c3de4719e66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5208, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005208__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5209-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5209, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "451906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5210, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5531", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5210", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "713ac93b-f0f3-4633-a258-0436439235c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5209, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005209__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5210-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5210, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5211, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5532", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5211", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e77499b9-1159-4ee3-b913-a0fbd6107d64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5210, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005210__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5211-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5211, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5212, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5533", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5212", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bba8ce1-64f7-434a-887b-69eccbb26d33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5211, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005211__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5212-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5212, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5213, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5534", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5213", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a92efbba-bcb5-4d81-8be1-e312ce64fc11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5212, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005212__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5213-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5213, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5214, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5535", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5214", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8380fb6-407a-4bc0-80b4-c935a22d8341", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5213, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005213__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5214-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5214, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5215, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5536", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5215", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6b8c23c-2fb8-4a25-a585-b8e33b637b19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5214, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005214__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5215-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5215, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5216, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5537", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5216", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fec8597f-9c31-4914-9e07-2bf1a311523d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5215, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005215__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5216-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5216, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5217, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5538", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5217", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "672d5c56-4f1b-4c76-9ce3-fb63189dbe50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5216, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005216__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5217-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5217, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5218, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5539", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5218", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46f276b2-8a3d-4270-a7a8-a7ed536ffa0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5217, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005217__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5218-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5218, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5219, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5540", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5219", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eee727f7-d7fd-45fa-85e7-380e85c3c33a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 452908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5218, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005218__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5219-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5219, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5220, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5541", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5220", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39c30a55-0b44-43e4-a66e-737876ff20e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5219, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005219__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5220-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5220, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5221, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5542", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5221", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9486549-22b8-460a-a7aa-3bf9712608a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5220, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005220__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5221-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5221, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "452953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5222, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5543", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5222", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa662665-8e0e-4d71-8b4e-367008cf5e14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5221, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005221__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5222-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5222, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5223, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5544", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5223", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a3d08f2-1eb9-43ac-a388-9eaf1cc8d5f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5222, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005222__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5223-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5223, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5224, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5545", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5224", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9db0d2c7-40eb-4b6c-9e36-3da5700e919a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5223, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005223__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5224-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5224, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5225, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5546", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5225", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9bc8ab09-1625-430b-8b83-e1f94fb806e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5224, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005224__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5225-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5225, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5226, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5547", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5226", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5014b81c-ea61-44f2-a280-20f0103630ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5225, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005225__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5226-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5226, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5227, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5548", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5227", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44932c3f-c733-415d-bb15-907359a1ca09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5226, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005226__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5227-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5227, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5228, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5549", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5228", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "671ddf4e-96a1-4d46-b866-0babb4a7ed7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5227, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005227__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5228-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5228, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5229, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5550", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5229", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41d680ed-c13c-428f-816d-0d60e46572ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5228, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005228__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5229-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5229, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5230, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5551", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5230", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41dca316-cd08-4302-b0cf-800cb6a0e17f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5229, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005229__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5230-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5230, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5231, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5552", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5231", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07baa783-da31-40e0-83a1-81701df47fc7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 453955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5230, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005230__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5231-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5231, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5232, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5553", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5232", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e22da7d-d51a-4c50-ba71-36e964e65866", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5231, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005231__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5232-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5232, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5233, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5554", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5233", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6dd1b9c1-e4a6-4b3c-bf59-5752b83cfa77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5232, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005232__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5233-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5233, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "453984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5234, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5555", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5234", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15964396-912e-4480-a0f0-fb37289ecd06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5233, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005233__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5234-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5234, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5235, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5556", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5235", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01d6b212-9ab0-4751-9617-e641d5e8bf0e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5234, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005234__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5235-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5235, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5236, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5557", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5236", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54e4eb30-0efe-4ff5-85dd-841dd784eaa0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5235, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005235__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5236-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5236, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5237, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5558", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5237", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0b866aa-9e4d-49fc-826b-26bc7070c225", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5236, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005236__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5237-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5237, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5238, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5559", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5238", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d287141e-d299-4b70-aaa1-af1e01a218cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5237, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005237__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5238-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5238, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5239, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5560", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5239", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc8852af-30b7-407b-8352-4480c7d3c0dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5238, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005238__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5239-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5239, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5240, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5561", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5240", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bef70ce-dc95-4cbe-ae7c-1fdb9343fd31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5239, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005239__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5240-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5240, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5241, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5562", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5241", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc824eba-58e7-4bb0-a4ce-31abe927ead9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5240, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005240__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5241-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5241, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5242, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5563", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5242", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09d3139b-6996-4a82-af8f-97f5d10da3c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5241, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005241__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5242-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5242, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5243, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5564", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5243", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "986f7c12-cee1-47ed-be8a-d3afeb4fb18a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 454986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5242, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005242__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5243-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5243, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5244, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5565", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5244", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c75d9e91-d240-4902-8734-b1753c8fc3df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5243, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005243__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5244-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5244, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "454937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5245, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5566", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5245", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22b6bc12-8da5-447c-aa2d-8595c0328259", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5244, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005244__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5245-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5245, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5246, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5567", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5246", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cebc2e3-18ab-46aa-a082-ee4ca96159c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5245, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005245__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5246-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5246, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5247, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5568", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5247", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a8e5cbf-778b-403c-8ca8-dfd896e7eed0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5246, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005246__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5247-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5247, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5248, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5569", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5248", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0928cbc9-7277-4912-9bf8-dc117fbd0668", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5247, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005247__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5248-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5248, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5249, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5570", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5249", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0acaeb78-c2e7-4f3e-8a49-5d071d3b48ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5248, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005248__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5249-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5249, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5250, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5571", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5250", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "689573b2-a975-4037-ae85-5c6619cfb142", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5249, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005249__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5250-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5250, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5251, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5572", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5251", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a542863a-09c9-44b7-a719-153d13d4f49b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5250, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005250__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5251-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5251, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5252, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5573", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5252", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c36a84b7-b99c-4477-8fdb-a2c88443b23b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5251, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005251__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5252-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5252, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5253, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5574", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5253", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76a208af-c1c4-45db-9d33-4c89ab3cae70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5252, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005252__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5253-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5253, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5254, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5575", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5254", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13856934-1008-4e3e-b258-f7e42d94962f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 455940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5253, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005253__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5254-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5254, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5255, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5576", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5255", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dad039d9-bedc-489d-94e3-fa91fdf9c1b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5254, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005254__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5255-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5255, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5256, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5577", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5256", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3182dc23-6d33-4da0-b2bb-e44951467e27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5255, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005255__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5256-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5256, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "455984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5257, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5578", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5257", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e8d1c19-101a-4456-b7e2-b9b33e50d87e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5256, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005256__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5257-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5257, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5258, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5579", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5258", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7233ad07-a639-4d64-860d-1e68a6d18d15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5257, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005257__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5258-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5258, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5259, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5580", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5259", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74c30d18-d3f2-4698-8e9e-60ba8e51d653", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5258, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005258__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5259-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5259, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5260, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5581", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5260", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9cb9d03a-583c-4f1c-8657-390b166236f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5259, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005259__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5260-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5260, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5261, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5582", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5261", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "622cba9e-418e-4c98-82bc-c1144ca28676", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5260, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005260__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5261-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5261, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5262, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5583", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5262", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ad4ee75-2887-4395-bd79-121cb5811a0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5261, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005261__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5262-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5262, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5263, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5584", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5263", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0decef7-ec03-4f2d-841b-9f39417c33ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5262, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005262__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5263-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5263, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5264, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5585", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5264", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "783ba3aa-07be-4467-9730-328cecb4a25e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5263, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005263__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5264-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5264, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5265, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5586", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5265", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d4cc426-be87-4cc2-8c6c-317d21cda7a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5264, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005264__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5265-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5265, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5266, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5587", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5266", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "576bea68-bb92-4b8e-bffe-2a51e3f38209", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5265, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005265__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5266-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5266, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5267, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5588", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5267", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91cf6ade-4e2f-4e64-8fc3-3684f2705fed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5266, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005266__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5267-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5267, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "456937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5268, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5589", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5268", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1006aad1-d59a-4bcb-b29c-70c1f3e5be27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5267, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005267__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5268-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5268, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5269, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5590", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5269", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04abcd57-1d71-4d65-ab08-66a277f84c56", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5268, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005268__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5269-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5269, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5270, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5591", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5270", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e86a57d0-30b5-4333-8c9d-f47b385f8226", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5269, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005269__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5270-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5270, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5271, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5592", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5271", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86edfd25-f59c-4665-bc79-23a4e081d668", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5270, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005270__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5271-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5271, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5272, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5593", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5272", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d46c17dc-4766-4489-9b3b-97fe699d32e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5271, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005271__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5272-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5272, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5273, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5594", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5273", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "78621f15-50b4-4d02-adfc-995a801dcd5c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5272, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005272__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5273-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5273, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5274, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5595", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5274", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1119a186-dad1-4f23-8b2f-fc4fd1e92a46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5273, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005273__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5274-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5274, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5275, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5596", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5275", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c26fad1c-a0a8-4f19-9017-b70d23404b85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5274, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005274__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5275-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5275, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5276, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5597", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5276", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8559878-eae4-4f3e-b21b-3be7e0bb619b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5275, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005275__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5276-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5276, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5277, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5598", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5277", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9ca4ed1-ab41-467b-a9ee-3c09189af6a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5276, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005276__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5277-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5277, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5278, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5599", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5278", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c45ce93-4460-43ab-acea-5b2372a6db10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5277, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005277__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5278-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5278, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5279, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5600", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5279", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41d844e1-c3e4-4cdb-8474-d71d939b7a4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5278, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005278__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5279-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5279, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "457984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5280, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5601", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5280", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f38ebae-8722-4d5a-aa6c-6c8fa88e5863", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5279, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005279__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5280-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5280, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5281, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5602", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5281", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "977bab7e-df45-467c-afcd-062d1ae3e135", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5280, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005280__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5281-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5281, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5282, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5603", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5282", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b5e6803-d2cf-4268-bffc-2a8299de8a8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5281, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005281__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5282-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5282, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5283, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5604", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5283", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d93cd12d-7134-4c31-8981-304017090880", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5282, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005282__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5283-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5283, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5284, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5605", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5284", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a13e4cb5-e03f-40e2-8a80-9f096b34e5f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5283, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005283__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5284-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5284, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5285, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5606", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5285", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e20ec99-4830-4bf9-b034-63b85b58dc2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5284, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005284__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5285-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5285, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5286, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5607", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5286", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "49b9ea6c-aafa-47e8-b115-8dcf8d3252d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5285, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005285__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5286-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5286, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5287, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5608", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5287", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cef1d80b-19c6-48a9-ae94-1b70aab4dc72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5286, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005286__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5287-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5287, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5288, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5609", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5288", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99bcced0-0ff1-4067-9f81-5c9627fa49ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5287, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005287__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5288-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5288, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5289, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5610", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5289", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cc2098c-5d2c-4bb7-af2d-c1f0de80535e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 458986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5288, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005288__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5289-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5289, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5290, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5611", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5290", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "93800be3-05ee-4db7-bb7d-b74fee681fc3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5289, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005289__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5290-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5290, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "458937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5291, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5612", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5291", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "579aa500-52a5-483e-964e-4ae124745060", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5290, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005290__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5291-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5291, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5292, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5613", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5292", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70cd777b-0514-4b3f-a7bc-4ccea2ca0e0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5291, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005291__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5292-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5292, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5293, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5614", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5293", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "260859b2-9460-4c8d-a2df-ce25e958c44d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5292, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005292__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5293-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5293, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5294, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5615", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5294", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ed522af-48a0-4eb8-8c30-32a5bc685cb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5293, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005293__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5294-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5294, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5295, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5616", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5295", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "744b4690-b32f-4104-b343-6a22089821cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5294, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005294__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5295-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5295, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5296, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5617", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5296", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28e2c763-4695-47a9-b504-a95aa146aab7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5295, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005295__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5296-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5296, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5297, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5618", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5297", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "183469e1-0492-4c05-ba92-e3e3437ea569", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5296, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005296__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5297-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5297, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5298, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5619", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5298", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0077a77b-d2be-45e5-8905-bd06d29217e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5297, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005297__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5298-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5298, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5299, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5620", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5299", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b3dce1a-0797-4991-9b73-1a868be46ea2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5298, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005298__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5299-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5299, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5300, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5621", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5300", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2c8015c-fc92-4851-a0af-fb6a162ec825", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 459940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5299, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005299__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5300-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5300, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5301, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5622", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5301", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2da4dac8-4ada-42f7-861a-a340cfb80f0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5300, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005300__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5301-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5301, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5302, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5623", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5302", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b638361a-89f2-4241-bb72-d63ed436994c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5301, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005301__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5302-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5302, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "459968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5303, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5624", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5303", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23a2e195-4b59-49a3-b2e2-4d709014e050", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5302, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005302__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5303-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5303, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5304, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5625", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5304", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "174fbf8c-c24d-4c8e-990b-9d6f04c7514c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5303, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005303__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5304-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5304, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5305, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5626", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5305", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dccd9d46-66e2-4ca9-8822-9aaf6b443ecf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5304, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005304__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5305-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5305, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5306, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5627", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5306", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e2b7158-18c4-45b2-851c-8fa71af38e96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5305, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005305__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5306-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5306, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5307, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5628", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5307", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dab07d68-e56e-4d04-ba18-2760f70706c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5306, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005306__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5307-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5307, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5308, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5629", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5308", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd1c7fdb-3a5d-46fe-b8fd-809a834dc329", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5307, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005307__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5308-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5308, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5309, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5630", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5309", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26bfcb0f-2ef8-4d93-8f40-298b7254eebd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5308, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005308__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5309-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5309, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5310, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5631", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5310", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb838a2c-aa82-4de4-a479-d664ab899b15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5309, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005309__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5310-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5310, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5311, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5632", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5311", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f083baa-2fdb-4fb3-a4a9-59cdb5bc9b10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5310, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005310__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5311-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5311, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5312, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5633", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5312", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8e9f553-abfe-4aea-8dfd-ed4fae2e0559", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 460971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5311, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005311__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5312-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5312, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5313, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5634", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5313", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f65ac87-ab97-45b7-9ed0-22d5fe1b15ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5312, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005312__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5313-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5313, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "460921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5314, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5635", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5314", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7608c93-ae96-4ca0-b2c1-6f7baf8fb572", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5313, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005313__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5314-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5314, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5315, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5636", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5315", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32176dd3-f9a2-423b-83b7-bc63acda11fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5314, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005314__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5315-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5315, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5316, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5637", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5316", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23d5ba54-4627-460b-b72f-8cb987a04c91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5315, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005315__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5316-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5316, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5317, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5638", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5317", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17095cd1-d1d2-4e53-9655-07ff71a295c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5316, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005316__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5317-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5317, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5318, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5639", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5318", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0781a47-676e-4fd1-a8f6-69b088798340", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5317, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005317__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5318-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5318, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5319, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5640", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5319", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "957e35dd-72c0-4eb9-b9c0-2e2753608c9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5318, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005318__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5319-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5319, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5320, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5641", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5320", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d29eac27-93d2-4e8b-a8ee-11b85f7a3647", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5319, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005319__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5320-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5320, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5321, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5642", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5321", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbdb88e1-29dc-47c2-b932-a00ea528a0f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5320, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005320__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5321-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5321, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5322, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5643", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5322", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e778e3b0-e197-40b3-9b95-217f10a3c3c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5321, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005321__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5322-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5322, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5323, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5644", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5323", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4d745f1-7b2b-4e7e-b01e-21f448dec48b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 461924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5322, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005322__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5323-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5323, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5324, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5645", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5324", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "203af34c-3a71-4164-a882-6a0e32a2bed1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5323, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005323__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5324-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5324, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5325, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5646", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5325", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e02f1e2-e485-48a0-83e7-2dffbf79f645", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5324, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005324__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5325-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5325, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "461968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5326, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5647", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5326", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22428e77-bee7-4b48-a103-6c3b379cdf93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5325, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005325__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5326-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5326, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5327, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5648", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5327", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d9fe2bbd-fc8b-4f4c-ba17-200488d2d56f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5326, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005326__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5327-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5327, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5328, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5649", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5328", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e81c984-8584-4f4f-af56-b419ad6d534c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5327, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005327__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5328-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5328, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5329, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5650", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5329", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb86c74f-75e3-43c0-a471-400632b4d149", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5328, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005328__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5329-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5329, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5330, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5651", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5330", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ad54c68-c8bf-45c9-9788-c50f5e9da082", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5329, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005329__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5330-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5330, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5331, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5652", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5331", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6f4af46-82a9-47d8-a167-32dbe81759ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5330, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005330__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5331-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5331, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5332, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5653", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5332", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1086fa6a-5f28-4caa-8d6b-511ad54d61e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5331, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005331__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5332-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5332, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5333, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5654", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5333", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "749cb969-c41b-45a6-887e-94e6373170ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5332, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005332__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5333-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5333, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5334, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5655", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5334", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccc2aa35-989d-49a8-ad03-8aa8c71b6015", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5333, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005333__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5334-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5334, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5335, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5656", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5335", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b79e881a-36a4-43d0-99d3-8838adbaac2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 462971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5334, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005334__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5335-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5335, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5336, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5657", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5336", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54a22e96-6ab2-489a-922e-2bb8c76a6895", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5335, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005335__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5336-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5336, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "462921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5337, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5658", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5337", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5379a443-b0e4-4ab0-96aa-b9246979bb42", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5336, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005336__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5337-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5337, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5338, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5659", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5338", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6bb8562-1b20-40ce-a444-f6f205bf600d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5337, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005337__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5338-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5338, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5339, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5660", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5339", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcb64280-400e-40f4-bc6a-80e1978dc483", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5338, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005338__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5339-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5339, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5340, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5661", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5340", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70237709-d627-4dd0-b237-c6fa1063fc71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5339, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005339__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5340-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5340, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5341, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5662", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5341", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4bc1923-04fc-41a1-9c71-9a27d30e441e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5340, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005340__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5341-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5341, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5342, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5663", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5342", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4aa78475-57ad-440f-9d97-eed6c1c5ec84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5341, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005341__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5342-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5342, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5343, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5664", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5343", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7337b107-147d-4140-84d0-719b61be5cab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5342, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005342__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5343-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5343, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5344, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5665", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5344", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb52542b-220b-4376-94f0-c4af5a4ed2aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5343, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005343__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5344-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5344, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5345, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5666", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5345", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27369caa-fee7-463f-b39d-a3bec2c325f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5344, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005344__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5345-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5345, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5346, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5667", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5346", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5e25b67-2e1b-4a20-8287-270d975b1bef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 463924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5345, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005345__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5346-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5346, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5347, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5668", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5347", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23d4e2e5-16c0-4148-8ffb-7c68f5d527c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5346, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005346__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5347-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5347, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5348, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5669", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5348", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4d0d8ed-914e-442b-bd94-78ab30cd9c0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5347, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005347__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5348-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5348, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "463968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5349, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5670", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5349", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "493a067c-788d-4eb9-a562-02aed7318147", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5348, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005348__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5349-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5349, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5350, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5671", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5350", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "534e2dea-6892-4c11-ba9b-9f570d8b0c31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5349, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005349__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5350-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5350, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5351, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5672", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5351", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60f71815-48c9-49dd-9941-f429f19562f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5350, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005350__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5351-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5351, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5352, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5673", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5352", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a51bc6e-fdd2-461f-8dd9-f652b3a4520e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5351, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005351__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5352-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5352, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5353, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5674", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5353", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8bbd5039-4e3a-486e-9a79-1ca7f1e0bee0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5352, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005352__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5353-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5353, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5354, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5675", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5354", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cc93693-9cb1-4fb7-9211-f8d87779fb0e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5353, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005353__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5354-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5354, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5355, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5676", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5355", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f15de99e-f6c5-4830-8d28-339b47d4cdd7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5354, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005354__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5355-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5355, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5356, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5677", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5356", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3255b5a6-da1a-4bd2-81c9-77013dd2a471", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5355, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005355__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5356-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5356, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5357, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5678", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5357", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5245ec97-6e66-4d4b-bf8c-df9bb14fb299", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5356, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005356__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5357-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5357, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5358, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5679", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5358", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6af6c7d7-d8f4-4051-9ca1-1f8afce832e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 464971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5357, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005357__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5358-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5358, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5359, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5680", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5359", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ddb1eaa-a7aa-4336-bca7-3b8858621ae4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5358, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005358__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5359-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5359, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "464921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5360, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5681", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5360", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d07d3d0-7c46-4525-8ee4-3da7a7126f2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5359, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005359__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5360-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5360, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5361, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5682", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5361", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae8d386e-f17a-4b5f-8178-bab77fd20d44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5360, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005360__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5361-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5361, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5362, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5683", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5362", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6ef4713-a2c1-4e74-93d1-b41f9aca438b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5361, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005361__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5362-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5362, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5363, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5684", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5363", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60648274-7a08-4b74-ad9e-757550a20036", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5362, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005362__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5363-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5363, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5364, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5685", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5364", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "249591c4-2c7f-4993-89f4-c175da02d216", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5363, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005363__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5364-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5364, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5365, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5686", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5365", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37a95a65-6c96-46cb-b6b7-cc1778795716", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5364, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005364__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5365-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5365, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5366, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5687", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5366", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f934ac64-c189-48c9-9140-64a153d81ab7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5365, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005365__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5366-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5366, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5367, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5688", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5367", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b2b0124-ffb5-42f8-a1ba-6af309027904", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5366, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005366__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5367-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5367, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5368, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5689", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5368", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f42ebaf-0e85-45a5-9693-69eea2ef2c1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5367, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005367__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5368-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5368, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5369, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5690", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5369", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "414ff081-b660-4cc7-8701-5f07cbfb5546", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 465924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5368, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005368__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5369-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5369, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5370, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5691", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5370", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e57aa414-67c0-4438-bfc3-d80c892fcb9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5369, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005369__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5370-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5370, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5371, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5692", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5371", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "779f749d-fb4c-457f-965b-00d9441b3b3a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5370, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005370__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5371-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5371, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "465953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5372, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5693", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5372", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8da3c8b-ba2b-4bbe-a95e-0df5732168ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5371, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005371__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5372-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5372, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5373, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5694", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5373", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "770f202c-e903-4ff0-9aae-4784b5e89868", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5372, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005372__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5373-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5373, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5374, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5695", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5374", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5212c8d-0848-4629-9c31-3979f04443d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5373, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005373__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5374-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5374, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5375, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5696", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5375", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fd14c52-834b-41cc-994e-6adeedc6ddf2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5374, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005374__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5375-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5375, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5376, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5697", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5376", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e84bb3a2-4da2-4beb-8a80-c6ffe1eeabe8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5375, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005375__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5376-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5376, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5377, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5698", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5377", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed3df494-82bb-41bb-ba41-fb929469e341", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5376, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005376__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5377-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5377, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5378, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5699", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5378", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d86102a6-0718-48a5-8113-e989d6e08a90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5377, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005377__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5378-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5378, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5379, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5700", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5379", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9baff220-e96c-4481-bc52-7acf9d1e4043", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5378, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005378__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5379-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5379, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5380, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5701", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5380", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97cd36a3-3f4d-456b-9450-e33af1b56532", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5379, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005379__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5380-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5380, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5381, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5702", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5381", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cd2d3ef-87f4-4128-a004-d999affe9558", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 466955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5380, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005380__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5381-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5381, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5382, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5703", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5382", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "441c66e3-715a-4c32-bedb-bb7687fd8bdf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5381, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005381__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5382-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5382, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "466906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5383, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5704", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5383", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2b3ce20-1fb5-4170-89a0-3fcaec64200f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5382, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005382__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5383-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5383, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5384, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5705", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5384", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "562b9de1-fc0c-4095-b93a-b0a0154c3af3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5383, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005383__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5384-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5384, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5385, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5706", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5385", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09c2b210-e4f7-4ce7-ba85-a9dd879be6f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5384, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005384__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5385-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5385, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5386, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5707", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5386", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04d89468-5a6b-49f2-9de5-2af132afad97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5385, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005385__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5386-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5386, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5387, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5708", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5387", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28200aeb-ac92-4791-b4fb-68e5efacd841", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5386, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005386__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5387-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5387, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5388, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5709", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5388", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7af4340b-3fc1-4d0d-8d70-88d7d758ddf5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5387, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005387__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5388-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5388, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5389, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5710", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5389", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6332c847-c336-4acb-abd3-c09dd90b3113", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5388, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005388__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5389-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5389, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5390, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5711", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5390", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4bf9129-4f12-422b-8532-5d38e6bc22de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5389, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005389__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5390-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5390, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5391, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5712", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5391", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64891514-5dc8-46a5-aa8f-c2cd5a88cf77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5390, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005390__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5391-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5391, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5392, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5713", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5392", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aae3dcb5-3ae8-4051-8df6-21d57c5ede37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 467908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5391, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005391__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5392-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5392, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5393, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5714", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5393", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fedf98c9-f431-4e2c-95fc-7dc40a005f6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5392, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005392__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5393-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5393, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5394, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5715", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5394", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6b00f1f-c538-4e09-a477-c1b8e45f9ca3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5393, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005393__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5394-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5394, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "467953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5395, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5716", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5395", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "325d3bcb-f7dd-4d26-a44e-61c121a15170", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5394, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005394__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5395-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5395, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5396, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5717", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5396", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c0ba21b-6359-4e8f-ae03-0ed7cbc856bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5395, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005395__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5396-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5396, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5397, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5718", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5397", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45a15453-b615-4397-ae88-26bdc178ed92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5396, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005396__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5397-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5397, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5398, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5719", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5398", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72915f98-a7a8-4a1d-a765-e202af12bd46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5397, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005397__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5398-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5398, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5399, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5720", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5399", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f671c64d-8d49-4f3c-a06b-6dd3a0207866", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5398, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005398__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5399-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5399, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5400, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5721", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5400", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3385f51b-249f-4516-8219-c3bec7d5675e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5399, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005399__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5400-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5400, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5401, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5722", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5401", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "593a7898-8738-4f44-a7dd-44a7f06ba0de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5400, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005400__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5401-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5401, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5402, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5723", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5402", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "679ee13d-418a-4b9e-894f-dc28ad978cc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5401, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005401__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5402-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5402, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5403, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5724", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5403", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "343b8764-f25e-4c8a-ab72-45d2958ea948", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5402, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005402__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5403-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5403, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5404, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5725", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5404", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "518f86bb-79d0-4a9c-b49b-20823de17635", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 468955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5403, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005403__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5404-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5404, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5405, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5726", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5405", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39666c2c-794b-4f5e-86c1-88c1b712abb4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5404, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005404__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5405-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5405, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "468906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5406, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5727", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5406", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaa0b00e-335a-4540-b25c-7aeaecd3e4e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5405, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005405__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5406-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5406, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5407, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5728", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5407", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b545ed7-760e-4901-ab0e-2a4392412519", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5406, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005406__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5407-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5407, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5408, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5729", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5408", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b185afab-8dea-4865-9914-62086f911967", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5407, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005407__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5408-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5408, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5409, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5730", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5409", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19d16ea5-b714-4dc6-a60c-8ba159768745", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5408, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005408__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5409-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5409, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5410, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5731", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5410", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cbaffb17-9335-4ef7-8b37-d779b49aec4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5409, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005409__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5410-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5410, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5411, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5732", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5411", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06db4fc0-07ac-40c7-8c7f-26c822c899c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5410, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005410__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5411-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5411, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5412, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5733", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5412", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18f1e12d-5c01-43d0-9793-02f70fe1b783", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5411, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005411__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5412-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5412, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5413, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5734", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5413", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a3de6f3-acbf-40b9-8d42-f83efd3b979b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5412, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005412__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5413-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5413, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5414, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5735", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5414", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4de7869-a477-40ef-8fbc-9471070f7c91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5413, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005413__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5414-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5414, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5415, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5736", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5415", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6670efb2-665b-49a3-bf06-aab3d4e2c3d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 469908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5414, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005414__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5415-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5415, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5416, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5737", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5416", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b962094b-652a-4718-aeb8-7496d42bcda8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5415, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005415__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5416-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5416, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5417, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5738", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5417", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c7d714f-a1a1-411f-9889-99187dbad5ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5416, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005416__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5417-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5417, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "469953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5418, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5739", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5418", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b01bb90c-2f8b-4968-87d4-59f39a263f77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5417, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005417__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5418-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5418, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5419, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5740", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5419", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ef71995-4236-4cfa-8fa6-4afdcb49a401", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5418, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005418__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5419-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5419, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5420, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5741", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5420", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c3e30a6-db9e-40a1-bcd4-ab874184e23e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5419, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005419__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5420-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5420, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5421, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5742", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5421", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66f0e60c-f7c5-4a18-bb47-286b6e242ed3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5420, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005420__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5421-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5421, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5422, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5743", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5422", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74516f78-8dd3-44d9-a597-2f1fde55238d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5421, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005421__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5422-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5422, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5423, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5744", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5423", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab0b06a9-0987-462a-a42a-5fcfd57f623f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5422, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005422__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5423-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5423, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5424, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5745", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5424", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df6f85e1-a0ae-4503-871e-133b0eb6afd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5423, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005423__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5424-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5424, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5425, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5746", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5425", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec0aaddf-b63c-457a-b70e-5766ba308acf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5424, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005424__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5425-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5425, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5426, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5747", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5426", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15c3ee87-0827-4863-b107-b643cd2b9041", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5425, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005425__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5426-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5426, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5427, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5748", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5427", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0467274-b371-41f7-98e5-35309268a40f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 470955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5426, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005426__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5427-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5427, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5428, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5749", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5428", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e827ecfa-f553-4bd8-a40d-d6dc762c2433", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5427, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005427__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5428-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5428, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5429, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5750", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5429", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a4d2d1b-1a05-4216-b5b6-7102796c0b2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5428, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005428__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5429-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5429, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "470984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5430, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5751", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5430", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae2f9bd2-22e8-436c-8bf6-a497799d95f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5429, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005429__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5430-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5430, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5431, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5752", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5431", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5beeab9-a0f9-4b34-95dc-cb5c09a2a2a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5430, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005430__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5431-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5431, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5432, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5753", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5432", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca0f29a8-e44e-49b1-8118-a862d596b882", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5431, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005431__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5432-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5432, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5433, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5754", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5433", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3309c1f9-bfde-4e9e-8717-036d268b8d6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5432, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005432__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5433-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5433, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5434, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5755", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5434", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44d00ce6-429d-4bc1-802b-af42a8c0dcfd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5433, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005433__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5434-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5434, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5435, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5756", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5435", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f6284f0-3fcf-4c46-accd-6764ef38e549", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5434, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005434__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5435-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5435, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5436, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5757", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5436", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76416b77-b8e2-43cf-b952-7005cdc65f36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5435, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005435__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5436-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5436, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5437, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5758", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5437", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13ada676-50af-46ce-8a01-1e0df24cb915", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5436, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005436__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5437-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5437, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5438, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5759", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5438", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55c6cced-7201-46c9-b051-669e754e190e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5437, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005437__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5438-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5438, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5439, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5760", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5439", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4554ea88-b087-42a4-836d-ffc85dd9672b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 471986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5438, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005438__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5439-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5439, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5440, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5761", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5440", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a88a45c1-ac14-4846-a706-4788e3e68b62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5439, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005439__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5440-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5440, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "471937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5441, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5762", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5441", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48b06561-0b26-40a4-8cea-d931c9df7fb5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5440, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005440__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5441-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5441, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5442, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5763", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5442", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5142d85e-7f1f-42cc-9d42-c853bd5625b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5441, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005441__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5442-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5442, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5443, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5764", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5443", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed6fc339-25c1-4bfc-aded-edcfa50d90d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5442, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005442__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5443-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5443, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5444, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5765", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5444", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00f33a40-ac45-48c8-a259-b0e71300b141", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5443, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005443__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5444-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5444, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5445, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5766", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5445", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d18eb93-8939-4565-a601-f683a12185cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5444, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005444__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5445-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5445, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5446, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5767", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5446", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e04904c3-7e5b-451c-ba8a-bc20e49c7d8c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5445, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005445__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5446-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5446, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5447, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5768", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5447", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e608a89-e8d8-464c-b3e4-45e75750a330", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5446, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005446__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5447-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5447, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5448, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5769", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5448", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65874393-49e9-4b84-b3cc-5ba5a779ac1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5447, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005447__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5448-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5448, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5449, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5770", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5449", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f52cabcd-2d04-4ea8-8a3d-5c7fda790d68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5448, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005448__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5449-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5449, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5450, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5771", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5450", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3463775a-0607-4aad-a2aa-6be891693356", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 472940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5449, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005449__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5450-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5450, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5451, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5772", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5451", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "999e9f88-29ac-43eb-9e3f-c332031ab3fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5450, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005450__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5451-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5451, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5452, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5773", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5452", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29cbf603-30b5-476c-b74e-542b74caec91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5451, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005451__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5452-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5452, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "472984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5453, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5774", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5453", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bce4759-471d-458e-9d71-2ec2697e881a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5452, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005452__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5453-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5453, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5454, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5775", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5454", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f22d5b0-2ab9-43a7-8da0-cfbeb85ea45b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5453, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005453__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5454-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5454, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5455, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5776", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5455", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91b111d8-f167-4a1c-bc48-3910617ec08d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5454, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005454__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5455-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5455, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5456, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5777", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5456", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8bed9841-f9df-4c51-a2d5-641c1f67aa0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5455, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005455__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5456-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5456, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5457, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5778", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5457", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0993f91c-2e2f-4557-833e-111b71082cb4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5456, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005456__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5457-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5457, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5458, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5779", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5458", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2a454f2-096f-406f-ade4-42aa25249d8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5457, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005457__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5458-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5458, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5459, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5780", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5459", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a65f31cd-5641-410a-afed-97905bc8fb0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5458, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005458__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5459-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5459, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5460, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5781", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5460", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c18bb37-6711-457c-9c2d-b408c57983a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5459, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005459__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5460-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5460, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5461, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5782", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5461", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8158b21d-e3e7-4b37-9bf4-2e1970dff1db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5460, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005460__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5461-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5461, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5462, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5783", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5462", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b58648ba-f945-422d-aa2b-ea7191840e68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 473986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5461, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005461__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5462-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5462, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5463, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5784", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5463", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aea78465-4c88-48c7-80d0-bfce9382597f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5462, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005462__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5463-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5463, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "473937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5464, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5785", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5464", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf1213fc-7d4e-48ce-9134-495c2702f765", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5463, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005463__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5464-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5464, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5465, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5786", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5465", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96cb134e-036e-4e5f-8480-95a077a51f03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5464, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005464__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5465-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5465, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5466, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5787", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5466", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "888e8f72-af88-43f1-a18f-418d9ce449b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5465, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005465__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5466-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5466, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5467, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5788", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5467", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bac17534-574c-4ab6-aa31-b596d2e849ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5466, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005466__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5467-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5467, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5468, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5789", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5468", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c92713a-8fb5-430f-b82a-183927179354", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5467, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005467__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5468-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5468, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5469, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5790", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5469", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe30a9c1-2cd6-4c47-b3f3-758b93c3cb8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5468, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005468__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5469-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5469, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5470, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5791", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5470", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19791b28-86b3-4e2a-8b4a-327972b2809d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5469, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005469__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5470-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5470, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5471, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5792", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5471", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82b96f95-ca91-440d-a38f-62a2b1390390", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5470, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005470__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5471-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5471, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5472, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5793", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5472", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a645d60d-17ff-4ae3-8b68-a1a006dcaf80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5471, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005471__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5472-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5472, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5473, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5794", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5473", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d30ae5a3-aaae-4696-97f0-373fa7a8c4c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 474940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5472, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005472__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5473-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5473, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5474, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5795", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5474", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58659710-0765-4f7c-8808-9b019f0e1baa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5473, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005473__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5474-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5474, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5475, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5796", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5475", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b2fe8b6-148a-4b93-b8fa-4d3a11810b9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5474, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005474__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5475-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5475, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "474984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5476, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5797", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5476", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2d15194-e619-4d06-be13-28f0da4e478d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5475, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005475__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5476-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5476, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5477, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5798", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5477", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1b6ccf9-c150-4820-9ad2-111cdbb69f6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5476, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005476__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5477-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5477, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5478, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5799", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5478", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "909ddd41-0641-49ae-9c3c-99ffc3be2cb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5477, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005477__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5478-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5478, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5479, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5800", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5479", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c9032871-6b8e-4774-8ac7-504929f975c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5478, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005478__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5479-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5479, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5480, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5801", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5480", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a667fa50-7d77-4f57-b210-7c176d39a5bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5479, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005479__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5480-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5480, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5481, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5802", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5481", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a289bc99-200b-4eda-9f8a-8fa79d79c001", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5480, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005480__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5481-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5481, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5482, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5803", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5482", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3360be86-607b-4956-9c3e-577251779daf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5481, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005481__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5482-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5482, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5483, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5804", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5483", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "994c1817-eb2f-4ed4-8df0-0e4d1f51fb41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5482, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005482__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5483-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5483, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5484, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5805", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5484", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4ee0170-b9ea-448d-9736-1edc8c210e39", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5483, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005483__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5484-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5484, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5485, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5806", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5485", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d10a547-5115-4162-9488-ac193b4ab16e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 475986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5484, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005484__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5485-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5485, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5486, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5807", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5486", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4c0c71e-a8e4-449d-a9cf-ed1bdd704824", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5485, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005485__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5486-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5486, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "475937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5487, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5808", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5487", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f8b2014-aff2-4541-92fe-ce825faec55b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5486, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005486__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5487-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5487, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5488, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5809", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5488", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "915da026-5600-4511-9d8f-f1543911e797", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5487, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005487__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5488-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5488, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5489, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5810", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5489", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dc568ca-b0fa-4ef3-b8fe-546a6a476129", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5488, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005488__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5489-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5489, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5490, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5811", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5490", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2cbde7e4-ffd4-4e22-a089-8857e343bf22", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5489, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005489__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5490-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5490, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5491, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5812", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5491", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed174402-aa83-4aa8-b4bc-2b5f67790261", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5490, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005490__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5491-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5491, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5492, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5813", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5492", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d544e461-2958-4cba-934a-871cd5c452ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5491, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005491__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5492-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5492, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5493, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5814", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5493", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d34fc852-dd8e-40e5-b62a-f6481c19adb5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5492, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005492__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5493-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5493, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5494, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5815", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5494", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4eb76e38-b746-44a0-83e0-8a5a1d45d2ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5493, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005493__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5494-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5494, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5495, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5816", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5495", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "733173cd-0090-4824-a4a5-3b2e73a7c1d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5494, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005494__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5495-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5495, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5496, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5817", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5496", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85ed78f9-736b-4361-b55e-af683448d504", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 476940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5495, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005495__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5496-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5496, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5497, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5818", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5497", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04340165-e936-4d1c-a869-670ea8ddd26e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5496, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005496__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5497-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5497, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5498, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5819", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5498", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "528c2834-da9d-4dbb-a076-4aa944e181e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5497, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005497__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5498-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5498, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "476968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5499, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5820", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5499", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50d265af-ed69-4d07-8181-cab68cf28937", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5498, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005498__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5499-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5499, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5500, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5821", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5500", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9cc198a0-ab96-470b-a738-afce11747af9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5499, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005499__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5500-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5500, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5501, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5822", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5501", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3001067e-bb4f-4da3-bd22-6ccdca2765cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5500, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005500__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5501-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5501, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5502, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5823", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5502", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b63bba2-f9e0-42cd-97fd-b9c50e43fbb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5501, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005501__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5502-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5502, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5503, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5824", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5503", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25c0174c-5664-46e1-9e12-efc89cfb24f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5502, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005502__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5503-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5503, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5504, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5825", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5504", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3fda7cc-1ddc-4e7f-9c76-e4032bb94fe7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5503, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005503__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5504-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5504, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5505, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5826", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5505", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77c737c2-bfc6-46ff-86c2-8e6fffc434cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5504, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005504__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5505-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5505, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5506, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5827", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5506", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de62fb0e-71b6-4586-bf5d-6a416f9a642c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5505, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005505__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5506-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5506, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5507, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5828", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5507", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59f15e8b-6084-426b-92ff-8ad3f448970e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5506, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005506__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5507-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5507, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5508, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5829", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5508", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8fe1f4dc-da00-4e62-b20e-3fe692789139", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 477971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5507, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005507__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5508-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5508, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5509, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5830", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5509", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff6d4075-9aaa-455c-b632-73875d808fe1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5508, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005508__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5509-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5509, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "477921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5510, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5831", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5510", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3be6c02-90c9-4840-ad8a-86c25aa3333f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5509, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005509__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5510-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5510, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5511, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5832", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5511", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "134e019e-1c19-46cc-9653-675ff01d5321", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5510, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005510__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5511-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5511, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5512, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5833", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5512", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "750864c5-a179-493f-8bf6-c9510a4dd74a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5511, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005511__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5512-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5512, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5513, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5834", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5513", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c338aa37-e0d6-4f86-9c20-6d304eb2c405", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5512, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005512__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5513-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5513, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5514, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5835", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5514", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3a7a820-6726-48c4-b75c-12f8efb76bc6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5513, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005513__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5514-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5514, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5515, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5836", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5515", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95fe6aff-0c65-4ae7-bd9e-99c926b14adc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5514, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005514__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5515-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5515, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5516, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5837", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5516", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39417ec9-74bf-434c-8253-d2724469550a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5515, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005515__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5516-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5516, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5517, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5838", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5517", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c73e5bf-06a0-4e48-8833-ae74e66e831d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5516, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005516__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5517-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5517, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5518, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5839", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5518", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8456de07-0562-4d51-a8c8-019ac5e17516", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5517, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005517__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5518-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5518, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5519, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5840", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5519", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71b10cc4-e68f-4e7f-b034-1a79fb41dcfb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 478924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5518, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005518__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5519-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5519, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5520, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5841", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5520", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64f650c5-bc44-4527-bbc1-61294af0edc3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5519, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005519__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5520-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5520, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5521, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5842", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5521", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d7b5d93-a2d4-484a-b818-81dae652d038", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5520, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005520__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5521-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5521, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "478968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5522, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5843", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5522", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0431d77-cec3-4639-8463-8be0ea6fe4fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5521, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005521__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5522-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5522, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5523, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5844", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5523", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44191a5b-7fd5-4b90-99b7-79b69cd9979b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5522, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005522__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5523-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5523, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5524, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5845", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5524", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a4223af-2688-4f18-8f6c-c03c0e052535", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5523, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005523__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5524-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5524, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5525, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5846", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5525", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc4ac8c8-61a4-4de3-b998-6e2547a141ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5524, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005524__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5525-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5525, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5526, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5847", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5526", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a78593e-9089-4ca3-9467-3ee24d8998fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5525, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005525__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5526-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5526, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5527, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5848", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5527", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8995b752-8f6f-4b55-9dc1-02a8749ec49e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5526, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005526__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5527-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5527, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5528, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5849", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5528", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fbbaca7-3101-45ee-81c9-aaddd06985cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5527, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005527__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5528-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5528, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5529, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5850", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5529", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8021fa1-35b2-4799-8ede-4d6d643b7e65", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5528, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005528__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5529-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5529, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5530, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5851", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5530", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e4ccce6-b656-4026-baa0-201f9bd3083c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5529, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005529__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5530-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5530, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5531, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5852", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5531", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8de4b92-8919-457a-9a63-27049fa71aea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5530, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005530__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5531-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5531, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5532, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5853", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5532", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "daf22927-af36-431a-974e-81abc9ce4457", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5531, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005531__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5532-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5532, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "479921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5533, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5854", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5533", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12dd01cf-2fda-443e-b409-a2ca3a03487a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5532, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005532__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5533-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5533, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5534, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5855", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5534", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51388b27-6c61-4d87-ace9-9cf8ccdcc4e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5533, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005533__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5534-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5534, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5535, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5856", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5535", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "393bc38e-0f47-435c-a1b0-e40f2848ba9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5534, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005534__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5535-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5535, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5536, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5857", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5536", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29ddcf11-f812-4ade-aaf9-33cafb1243f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5535, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005535__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5536-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5536, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5537, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5858", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5537", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac1cdb19-9e00-40ac-a0c7-c48a4d6bf43a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5536, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005536__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5537-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5537, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5538, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5859", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5538", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6509d469-9945-460d-9a7f-52cbfbb33471", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5537, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005537__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5538-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5538, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5539, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5860", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5539", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34fcc9be-3cf7-4cdd-bf17-245e8408119e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5538, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005538__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5539-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5539, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5540, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5861", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5540", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a8c1f4c-416d-4651-bcf4-2c7820c7c1cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5539, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005539__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5540-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5540, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5541, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5862", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5541", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2c1b7b7-3110-4602-970a-87fec9220025", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5540, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005540__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5541-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5541, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5542, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5863", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5542", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7a21c29-1c12-4d70-868f-2ee9ae243c08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5541, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005541__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5542-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5542, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5543, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5864", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5543", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c84e5c0b-1101-4e2f-a9c8-4e89594a3bbf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5542, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005542__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5543-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5543, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5544, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5865", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5544", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "973a606b-32fa-4ba9-a072-93aa3041225d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5543, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005543__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5544-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5544, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "480968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5545, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5866", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5545", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c243bec-c02e-4e59-8446-ebceac2a5b89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5544, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005544__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5545-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5545, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5546, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5867", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5546", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56b15b02-d3fd-44ab-92d7-552965afa0f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5545, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005545__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5546-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5546, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5547, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5868", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5547", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fceeb7c5-b8cb-4484-9f46-837ba766c2a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5546, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005546__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5547-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5547, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5548, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5869", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5548", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "578e980b-80aa-4ef0-b7d5-acf2cdbe3f40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5547, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005547__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5548-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5548, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5549, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5870", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5549", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e850834a-c2e3-411e-b02b-5f10cdccfa1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5548, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005548__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5549-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5549, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5550, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5871", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5550", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3611e48b-241f-4ec2-a1f4-2cfa9656c0ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5549, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005549__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5550-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5550, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5551, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5872", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5551", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75465866-6151-4a4c-8b77-bd79e893b4ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5550, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005550__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5551-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5551, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5552, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5873", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5552", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f94f4a6-e969-4a21-9bd9-14913920ad0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5551, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005551__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5552-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5552, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5553, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5874", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5553", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8956b22c-4166-48b3-b062-98e254d9783d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5552, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005552__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5553-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5553, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5554, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5875", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5554", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83ccaa5f-e22f-4596-ba9f-f7cae9fe9a26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 481971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5553, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005553__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5554-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5554, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5555, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5876", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5555", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b14e529d-40d1-414e-bd73-0c9a5cb4ffd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5554, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005554__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5555-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5555, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "481921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5556, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5877", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5556", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4da7bc98-48d8-4957-8384-8e6ad65b3294", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5555, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005555__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5556-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5556, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5557, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5878", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5557", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7bf6ecc3-b648-4863-ae50-e711188df088", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5556, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005556__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5557-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5557, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5558, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5879", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5558", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0885546-9fee-4263-946b-fd6d25b45afb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5557, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005557__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5558-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5558, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5559, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5880", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5559", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a8bd2ca6-cd2c-4cad-b498-8ea5321a5714", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5558, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005558__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5559-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5559, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5560, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5881", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5560", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d20d57f-3637-4674-99d1-257684f522ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5559, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005559__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5560-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5560, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5561, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5882", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5561", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d29241ae-4792-4635-89bd-76430afb8e5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5560, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005560__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5561-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5561, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5562, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5883", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5562", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85de4fe4-3160-47cb-ac4b-873575b47bd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5561, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005561__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5562-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5562, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5563, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5884", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5563", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6895ac56-56d0-4d2f-b7e5-64a608dcf312", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5562, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005562__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5563-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5563, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5564, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5885", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5564", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2999ebfa-a28a-4dc9-8ddd-395e5d47c2f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5563, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005563__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5564-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5564, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5565, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5886", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5565", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43162c67-1b2d-4147-a665-6204b00ace7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 482924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5564, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005564__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5565-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5565, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5566, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5887", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5566", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cdb10d8-ad50-42c5-a1b3-43e8e46efc73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5565, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005565__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5566-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5566, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5567, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5888", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5567", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee3d8e57-f399-468f-a1e1-c100f556eb5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5566, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005566__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5567-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5567, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "482953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5568, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5889", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5568", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d41511fc-e24b-4e43-81cc-4f9962914b4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5567, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005567__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5568-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5568, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5569, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5890", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5569", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6911f578-f67e-449c-a50c-6b8516840a8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5568, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005568__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5569-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5569, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5570, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5891", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5570", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec91220e-25e8-4518-9132-3cf72063c44d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5569, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005569__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5570-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5570, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5571, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5892", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5571", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05899243-1419-4bb1-aeaf-1922ed08072a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5570, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005570__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5571-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5571, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5572, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5893", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5572", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d36c867-a76e-4030-bfac-9d0ba93d3dbd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5571, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005571__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5572-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5572, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5573, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5894", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5573", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e392a180-2645-45c6-bfe5-6f2e82023ac5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5572, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005572__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5573-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5573, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5574, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5895", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5574", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56e92464-9c52-4b99-ab9c-2f051496d093", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5573, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005573__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5574-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5574, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5575, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5896", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5575", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59dff9e6-2a21-44f1-ba49-54880f5f4bc6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5574, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005574__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5575-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5575, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5576, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5897", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5576", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62783718-7c31-47d6-9358-5223acf8b962", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5575, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005575__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5576-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5576, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5577, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5898", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5577", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da3d7729-1955-4301-832e-9084415f2ef6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 483955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5576, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005576__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5577-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5577, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5578, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5899", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5578", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8117ad2b-4697-4ec7-83e4-57cef022f544", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5577, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005577__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5578-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5578, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "483906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5579, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5900", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5579", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebf4cc1f-6976-4778-bd60-9d262a9febf5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5578, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005578__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5579-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5579, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5580, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5901", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5580", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8bea20e-991a-4ff9-938d-84f9e3669cc2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5579, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005579__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5580-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5580, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5581, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5902", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5581", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c15b350f-f6ed-4a14-802a-18a0393b4038", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5580, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005580__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5581-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5581, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5582, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5903", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5582", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adb68ae0-fa80-4eb5-a600-5fecda2e299f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5581, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005581__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5582-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5582, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5583, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5904", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5583", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8f8f9ad-a0dc-4438-938f-c7fc12958025", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5582, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005582__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5583-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5583, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5584, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5905", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5584", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c41f7400-9d0f-4299-998c-12cbe4b60dcc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5583, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005583__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5584-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5584, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5585, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5906", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5585", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "272a7d36-6e3f-43f6-8b01-3b3e95a960e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5584, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005584__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5585-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5585, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5586, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5907", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5586", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76b2d0f4-e31d-4b9f-974b-729cc85cff66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5585, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005585__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5586-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5586, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5587, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5908", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5587", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "375ac2f4-5d78-41a2-939c-c5a6d5e5a850", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5586, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005586__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5587-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5587, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5588, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5909", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5588", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b9b5a53-e078-47be-9fa9-4cc2bc360899", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 484908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5587, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005587__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5588-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5588, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5589, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5910", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5589", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a463050b-6a2a-4d1e-aeaa-fba3b4eb8689", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5588, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005588__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5589-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5589, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5590, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5911", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5590", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63f8e4c4-58b1-4cc6-bdb5-c2cf6401e1b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5589, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005589__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5590-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5590, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "484953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5591, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5912", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5591", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdaf1503-c236-4ed2-aa20-c07b18be503f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5590, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005590__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5591-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5591, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5592, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5913", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5592", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ff89769-5d4e-4bc3-af41-3888a8503209", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5591, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005591__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5592-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5592, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5593, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5914", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5593", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a015c8dd-f030-4106-9aa7-f4f33b2769db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5592, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005592__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5593-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5593, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5594, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5915", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5594", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "938ad169-517c-461d-9fc1-28055a1d3517", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5593, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005593__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5594-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5594, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5595, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5916", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5595", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37def5e9-6122-440a-b498-1f04874d7059", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5594, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005594__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5595-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5595, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5596, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5917", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5596", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0eb9d74-f198-44aa-a20c-42b62b7b6b17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5595, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005595__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5596-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5596, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5597, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5918", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5597", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e92ce1e-49dc-4267-b883-fc9182c15f6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5596, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005596__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5597-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5597, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5598, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5919", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5598", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c61e0b99-3946-4612-8d3f-02f7319a9f07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5597, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005597__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5598-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5598, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5599, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5920", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5599", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e7f5120-2229-4f69-a705-7c61d48523d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5598, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005598__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5599-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5599, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5600, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5921", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5600", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b603fe9e-789f-4485-8781-0d305ef1e364", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 485955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5599, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005599__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5600-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5600, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5601, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5922", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5601", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f38ff75-e1cb-4768-b998-de0b9d6f0e95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5600, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005600__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5601-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5601, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "485906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5602, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5923", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5602", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7d4e838-efa9-4836-998b-d8cad4b12ad1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5601, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005601__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5602-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5602, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5603, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5924", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5603", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c85e2d2-64fa-4262-84fe-2831e1bf4926", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5602, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005602__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5603-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5603, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5604, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5925", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5604", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bdfba91-3d16-4ce5-bcf5-443a9d6e1087", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5603, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005603__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5604-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5604, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5605, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5926", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5605", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59ee9f49-ad74-4fcc-be47-005996a84553", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5604, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005604__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5605-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5605, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5606, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5927", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5606", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "195c118e-1470-4660-ac19-b641d0d8808a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5605, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005605__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5606-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5606, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5607, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5928", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5607", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d201420a-d7b9-44b2-b975-8251e571c640", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5606, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005606__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5607-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5607, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5608, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5929", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5608", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84d41df6-261b-4135-bae4-1d2ecc289dd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5607, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005607__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5608-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5608, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5609, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5930", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5609", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ecbe985-eb4f-4242-b43e-9d41c7e2b8f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5608, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005608__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5609-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5609, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5610, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5931", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5610", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b94209aa-3c19-4ec0-9b8f-db96f8aa6823", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5609, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005609__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5610-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5610, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5611, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5932", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5611", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f151801-ae31-4d41-a10e-55dc8f9de401", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 486908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5610, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005610__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5611-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5611, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5612, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5933", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5612", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1070503-7763-4752-8800-3f92a88ddb66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5611, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005611__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5612-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5612, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5613, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5934", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5613", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c54475d-cc6f-4eb4-bd8e-d544329d833f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5612, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005612__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5613-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5613, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "486953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5614, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5935", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5614", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c44a0fd-1fc5-4f40-b68c-124fb673da79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5613, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005613__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5614-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5614, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5615, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5936", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5615", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "699c37db-1acc-4b84-94b2-77cdd066467c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5614, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005614__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5615-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5615, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5616, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5937", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5616", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc5bf67c-56e4-443c-b2fd-b5eed05c5db2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5615, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005615__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5616-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5616, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5617, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5938", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5617", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc7708e8-0251-4b6f-a84b-66da8e7b41e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5616, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005616__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5617-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5617, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5618, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5939", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5618", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a0cbfce-5c14-4a00-9e26-e20821ac960d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5617, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005617__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5618-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5618, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5619, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5940", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5619", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1235377-eef9-4b84-aca8-21df5310309e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5618, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005618__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5619-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5619, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5620, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5941", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5620", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a561c6e-1f8d-4868-b967-1d124bb9300d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5619, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005619__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5620-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5620, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5621, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5942", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5621", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3fb266d-f3a8-49c8-9827-2a81d5349b5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5620, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005620__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5621-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5621, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5622, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5943", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5622", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57e5f75a-baab-4b7d-922c-ba7873f2d96c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5621, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005621__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5622-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5622, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5623, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5944", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5623", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ec04362-23f0-42e8-abfe-0eb69000882c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 487955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5622, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005622__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5623-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5623, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5624, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5945", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5624", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a3e2276-4046-4ba7-99bb-5499854206c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5623, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005623__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5624-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5624, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5625, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5946", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5625", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ed35c14-b712-4637-8d2f-0ebcb3de7ec7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5624, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005624__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5625-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5625, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "487984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5626, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5947", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5626", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "feb6dbd3-381e-42b2-8ef4-f1252c588bcd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5625, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005625__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5626-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5626, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5627, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5948", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5627", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9413c7dc-509a-4c17-acba-8ac156572544", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5626, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005626__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5627-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5627, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5628, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5949", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5628", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f22fb0f5-a94a-4ba9-ae0c-fdad59235660", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5627, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005627__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5628-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5628, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5629, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5950", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5629", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9135384d-3153-4c50-97be-80f0fba18e59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5628, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005628__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5629-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5629, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5630, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5951", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5630", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0a50131-1ea6-4c95-a1ff-e7b59080e12e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5629, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005629__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5630-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5630, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5631, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5952", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5631", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e033b8f1-b05b-4a19-8228-fcef53800d43", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5630, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005630__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5631-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5631, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5632, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5953", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5632", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c80a0536-65b7-4672-8e25-466d6811808c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5631, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005631__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5632-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5632, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5633, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5954", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5633", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc1cdb3d-5841-45e9-b242-53365642fc15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5632, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005632__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5633-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5633, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5634, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5955", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5634", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54475d9d-2e70-4bbe-9b4a-8a52fe5e08a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5633, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005633__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5634-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5634, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5635, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5956", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5635", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "994f102b-7e2b-4b2c-8431-0fee1c34416e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 488986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5634, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005634__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5635-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5635, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5636, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5957", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5636", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a27fa94e-5b38-404d-a9af-440b4aba7ae4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5635, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005635__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5636-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5636, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "488937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5637, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5958", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5637", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efaaeaff-e7a2-4926-9769-d27a6d7d5c72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5636, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005636__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5637-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5637, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5638, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5959", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5638", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "089ac29a-b927-44db-8331-02be75cc8daa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5637, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005637__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5638-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5638, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5639, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5960", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5639", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bab8b08-b32b-4f1f-bdf1-4adb05d6bb1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5638, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005638__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5639-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5639, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5640, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5961", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5640", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "616af2b7-b22d-4be5-ab18-9b6aad50b7b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5639, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005639__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5640-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5640, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5641, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5962", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5641", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c110255a-8371-482f-9359-8ebec72fb88d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5640, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005640__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5641-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5641, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5642, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5963", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5642", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b089d458-f12a-43dd-a62d-a01fb8e292be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5641, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005641__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5642-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5642, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5643, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5964", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5643", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd45f2e6-3d0a-4383-bd4e-5f1370911a46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5642, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005642__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5643-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5643, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5644, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5965", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5644", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a88a8a6-d8c2-4c9b-bfc9-8f5d324ff065", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5643, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005643__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5644-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5644, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5645, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5966", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5645", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "acac9ae2-85e8-41d3-814a-482f8406ec20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5644, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005644__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5645-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5645, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5646, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5967", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5646", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fcd14b4-b2f8-4cb4-bc17-c489117b7869", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 489940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5645, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005645__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5646-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5646, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5647, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5968", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5647", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df16a818-3091-4efa-a9c8-98fb99e6f160", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5646, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005646__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5647-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5647, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5648, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5969", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5648", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af126845-f6bb-4cb8-841e-e070aec07824", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5647, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005647__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5648-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5648, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "489984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5649, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5970", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5649", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cdff9983-4165-4bdc-aa82-bfd1322d1982", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5648, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005648__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5649-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5649, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5650, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5971", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5650", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68c112bd-3d32-48db-88de-b240eced80e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5649, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005649__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5650-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5650, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5651, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5972", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5651", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2d30c47-e03d-4c59-81df-3e00a82f7332", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5650, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005650__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5651-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5651, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5652, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5973", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5652", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "423f442e-a3cf-4a43-b0a5-b4ee77a73aec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5651, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005651__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5652-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5652, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5653, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5974", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5653", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70d51b46-0859-4b7a-83e8-098cbb24135a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5652, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005652__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5653-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5653, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5654, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5975", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5654", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6de7a8c-2ca3-4812-9449-94983ef3771d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5653, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005653__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5654-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5654, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5655, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5976", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5655", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54865db3-973a-4621-942c-1fd957d7eb98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5654, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005654__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5655-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5655, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5656, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5977", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5656", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a03fc4d-905d-4c67-a827-ce7c214db5da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5655, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005655__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5656-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5656, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5657, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5978", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5657", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "456d4eba-8266-40ac-852b-ece947540c30", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5656, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005656__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5657-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5657, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5658, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5979", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5658", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4dd12dec-9e6e-4297-9bc7-b8c87344fd4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 490986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5657, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005657__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5658-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5658, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5659, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5980", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5659", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e40007d-56dd-44dd-822c-fc382d21767b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5658, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005658__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5659-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5659, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "490937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5660, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5981", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5660", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71c183b1-aaee-4b2b-8c40-b31a6bdfe3b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5659, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005659__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5660-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5660, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5661, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5982", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5661", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60e5e8b5-7ad1-4f56-9419-b6e68ee2830a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5660, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005660__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5661-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5661, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5662, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5983", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5662", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5065ece7-aef0-4dc6-b87d-8456a23493a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5661, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005661__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5662-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5662, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5663, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5984", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5663", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "49802a7e-5b44-4e74-a516-12e43d6f3b45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5662, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005662__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5663-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5663, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5664, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5985", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5664", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4707440-4d7d-4473-92a9-873d74727262", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5663, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005663__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5664-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5664, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5665, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5986", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5665", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fdd6b3fa-c11a-4fb5-adb9-64b82c7053e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5664, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005664__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5665-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5665, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5666, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5987", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5666", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "609ae561-d5a2-44ed-a3a5-c1b30c2eea98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5665, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005665__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5666-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5666, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5667, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5988", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5667", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d4b07a8-2771-486f-83e7-e69b9ef3e804", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5666, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005666__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5667-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5667, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5668, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5989", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5668", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ddbac88b-fa87-4e54-a7bb-c7d7c3d265d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5667, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005667__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5668-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5668, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5669, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5990", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5669", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18bf4a00-0701-4c1b-afc9-0635f09a5c7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 491940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5668, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005668__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5669-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5669, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5670, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5991", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5670", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef254cf9-bc97-41cf-93ee-77ae5db5cebd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5669, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005669__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5670-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5670, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5671, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5992", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5671", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb19a14c-1ae8-4694-b1e6-cfd6f8d8bfca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5670, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005670__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5671-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5671, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "491984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5672, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5993", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5672", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "792002c6-0aa8-43dd-b768-8b35e0f9c268", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5671, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005671__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5672-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5672, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5673, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5994", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5673", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a8b4384-ad0f-4d41-9d60-76e937c7575c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5672, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005672__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5673-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5673, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5674, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5995", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5674", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98ff0212-a3b2-4796-9502-f4da59b4d50e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5673, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005673__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5674-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5674, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5675, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5996", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5675", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ea3992d-44da-4eb9-b910-ec6fe4087874", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5674, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005674__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5675-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5675, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5676, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5997", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5676", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1a38710-c1bd-4f7e-a7f6-a287f55d6ba6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5675, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005675__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5676-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5676, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5677, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5998", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5677", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c078dff-5e0c-422a-b652-706dc1b77439", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5676, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005676__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5677-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5677, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5678, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "5999", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5678", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "557854bd-71e0-4a9e-99ed-9b9d11057a89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5677, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005677__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5678-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5678, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5679, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6000", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5679", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f71de50-ec8d-4bd1-a9d1-8bd3c50d6975", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5678, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005678__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5679-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5679, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5680, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6001", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5680", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34ddccc1-fdd0-4591-b6fc-697b35fa802d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5679, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005679__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5680-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5680, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5681, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6002", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5681", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f07bc18f-d950-4348-8ab4-8fea257d566a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 492986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5680, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005680__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5681-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5681, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5682, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6003", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5682", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27c0d53b-5c04-49f7-bfbd-2eef1c6eb44f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5681, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005681__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5682-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5682, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "492937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5683, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6004", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5683", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c475c290-242c-4df6-ac7a-12a8043dea97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5682, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005682__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5683-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5683, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5684, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6005", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5684", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7d73e6b-632b-466a-b0e4-f06b31c03519", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5683, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005683__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5684-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5684, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5685, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6006", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5685", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70e5e19d-77de-476d-ad73-d7bdf39ab68b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5684, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005684__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5685-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5685, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5686, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6007", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5686", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab254efe-6551-4364-a0e6-0501dd4f1848", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5685, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005685__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5686-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5686, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5687, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6008", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5687", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f453956d-544c-4ef2-8c6b-edbade1d41ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5686, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005686__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5687-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5687, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5688, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6009", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5688", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84b4c29c-fd30-4558-ad20-51db2b9b5085", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5687, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005687__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5688-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5688, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5689, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6010", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5689", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b285fb72-02bf-421e-aaf0-c966f5917949", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5688, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005688__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5689-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5689, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5690, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6011", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5690", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5de1d27-c395-4f01-92e8-93efa87ed0b3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5689, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005689__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5690-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5690, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5691, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6012", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5691", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfcfc215-1745-4daa-9833-32523353aace", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5690, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005690__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5691-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5691, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5692, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6013", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5692", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d72b58f6-3b91-4994-9a79-acf0c5cb9872", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 493940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5691, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005691__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5692-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5692, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5693, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6014", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5693", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1772c874-5b54-4d8a-923c-34a9464c8201", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5692, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005692__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5693-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5693, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5694, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6015", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5694", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09d7c01b-17b1-41e1-9c59-03a7fd16b857", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5693, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005693__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5694-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5694, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "493968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5695, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6016", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5695", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b6feaa3-cdc6-45eb-aabc-1ab477a409aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5694, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005694__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5695-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5695, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5696, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:26:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6017", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5696", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "668a2b88-0114-4647-a2c4-c022ac56b22f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5695, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005695__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5696-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5696, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5697, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6018", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5697", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01295061-4b1f-40e2-a01b-6129434e9bf1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5696, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005696__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5697-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5697, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5698, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6019", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5698", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83b03d90-34ab-477a-a4e8-34f189b96e47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5697, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005697__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5698-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5698, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5699, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6020", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5699", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d25c2c5f-87ed-47bc-8216-408331b2362a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5698, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005698__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5699-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5699, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5700, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6021", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5700", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cb60611-34bb-492d-b5e4-e020081e3e81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5699, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005699__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5700-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5700, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5701, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6022", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5701", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab3b756b-b60e-4ab2-b619-f62ee1c6c878", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5700, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005700__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5701-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5701, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5702, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6023", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5702", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c9d91b6-bd33-4812-a9b5-1e97081e7143", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5701, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005701__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5702-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5702, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5703, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6024", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5703", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96b8d461-1d9e-4152-86f0-9f01a6ef3dcb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5702, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005702__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5703-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5703, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5704, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6025", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5704", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23ccb009-9a34-4331-9431-d428aa482df1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 494971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5703, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005703__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5704-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5704, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5705, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6026", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5705", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1d44b0b-c8ce-48ab-a094-0bd4e34805f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5704, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005704__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5705-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5705, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "494921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5706, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6027", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5706", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2a6192a-dd63-4f4e-8085-6adcbf3754e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5705, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005705__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5706-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5706, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5707, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6028", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5707", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d12d59dd-642b-4a03-963e-4c583556bfbc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5706, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005706__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5707-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5707, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5708, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6029", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5708", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3618ca0-df40-4cd6-bba6-b29f1fb3f58b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5707, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005707__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5708-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5708, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5709, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6030", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5709", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa74ee8c-b6ae-431c-a00a-a2fd1344e443", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5708, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005708__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5709-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5709, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5710, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6031", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5710", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0266971c-7c5c-428b-b025-07f4b15feb84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5709, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005709__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5710-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5710, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5711, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6032", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5711", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d4ccef6-fe64-409d-b175-3d5a0f9241f5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5710, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005710__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5711-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5711, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5712, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6033", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5712", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46391abf-3aee-444a-896d-8a54d0d189bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5711, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005711__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5712-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5712, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5713, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6034", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5713", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19a93140-9608-4701-82ea-72fe30991aee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5712, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005712__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5713-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5713, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5714, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6035", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5714", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fc6837b-7fd7-4ebc-8744-18aefb3e2f0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5713, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005713__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5714-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5714, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5715, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6036", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5715", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e664fe1-8399-46bb-927f-38cbc85ab2fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 495924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5714, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005714__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5715-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5715, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5716, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6037", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5716", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b4b1344-8ba7-4648-b179-728e5b23cadd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5715, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005715__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5716-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5716, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5717, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6038", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5717", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63ae429f-e2bf-4f7f-b06b-f16ca1f2b649", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5716, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005716__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5717-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5717, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "495968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5718, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6039", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5718", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a36f9215-3d2c-4f85-9af7-c48289d03cc5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5717, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005717__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5718-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5718, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5719, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6040", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5719", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f90bb2ba-5fe1-44bb-a596-25b71bb99e67", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5718, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005718__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5719-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5719, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5720, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6041", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5720", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31a8abcd-b80c-48bc-acf3-0b03b3d34bca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5719, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005719__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5720-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5720, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5721, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6042", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5721", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "213513a6-3356-4560-8fae-2019cd8deb88", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5720, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005720__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5721-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5721, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5722, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6043", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5722", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb18392f-1385-429b-a084-0fe8ea3fccba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5721, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005721__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5722-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5722, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5723, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6044", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5723", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75843bc1-815d-43c9-bfd3-f05dd5a1cda7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5722, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005722__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5723-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5723, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5724, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6045", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5724", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaafaf11-bb03-4a19-9675-77489a0daf25", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5723, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005723__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5724-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5724, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5725, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6046", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5725", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7cafda01-5861-4862-a8d0-e5aa7875121e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5724, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005724__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5725-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5725, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5726, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6047", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5726", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf59882c-8cfb-4fd3-9500-fdbd10dd0a70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5725, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005725__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5726-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5726, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5727, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6048", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5727", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "260c1e1f-71bc-46b0-ae49-26ba127e876f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 496971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5726, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005726__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5727-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5727, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5728, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6049", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5728", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2f55094-a08e-4412-8921-f6e4af2df7f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5727, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005727__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5728-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5728, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "496921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5729, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6050", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5729", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efb6c424-f9fa-4977-95bc-16e2c8cd1606", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5728, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005728__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5729-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5729, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5730, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6051", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5730", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb3e1be4-642b-4fe3-8159-0a3ad0b6d2ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5729, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005729__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5730-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5730, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5731, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6052", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5731", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a452d564-0132-46f2-940e-0cca1870b238", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5730, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005730__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5731-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5731, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5732, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6053", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5732", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f52f4a7-c660-4afd-b352-80a234126b85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5731, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005731__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5732-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5732, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5733, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6054", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5733", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18f3222c-473d-45fc-a191-62495b5e382a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5732, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005732__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5733-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5733, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5734, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6055", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5734", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4466b3b1-323e-4cf7-8879-1bfc90c0782a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5733, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005733__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5734-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5734, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5735, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6056", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5735", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1aa0383c-a215-4251-b360-b30abaae1db7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5734, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005734__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5735-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5735, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5736, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6057", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5736", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5f7be52-04d4-448e-944a-7dbd7c2fe426", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5735, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005735__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5736-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5736, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5737, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6058", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5737", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24640ada-f713-4c95-bbe7-ee2de4fb6d8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5736, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005736__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5737-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5737, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5738, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6059", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5738", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "843e6155-5bbf-4d93-863e-0562e23979a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 497924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5737, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005737__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5738-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5738, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5739, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6060", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5739", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7491b71c-7bb8-44ed-9381-d0ba34327b03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5738, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005738__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5739-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5739, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5740, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6061", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5740", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a65822a-d04a-463a-9c15-d53fd8eb3fa7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5739, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005739__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5740-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5740, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "497968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5741, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6062", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5741", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "649fe92d-57e7-4d10-929a-c1add59f9a4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5740, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005740__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5741-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5741, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5742, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6063", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5742", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d7b45ab-d4a0-467d-9445-d49981d1e0db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5741, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005741__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5742-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5742, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5743, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6064", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5743", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54ecf4a9-4d27-472f-8455-55c1ec08b983", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5742, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005742__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5743-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5743, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5744, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6065", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5744", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42084f88-c78d-45c9-b6c3-be0731eed146", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5743, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005743__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5744-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5744, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5745, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6066", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5745", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63ea0664-616f-4807-bf96-9eacda8e63be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5744, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005744__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5745-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5745, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5746, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6067", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5746", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00044bb1-ee9f-443f-ae97-5a6b1e4bfadc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5745, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005745__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5746-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5746, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5747, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6068", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5747", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4fe9035-2bfe-4919-a09a-a07055c8ee5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5746, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005746__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5747-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5747, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5748, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6069", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5748", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "376c15d5-935d-4fd9-be53-27f076389295", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5747, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005747__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5748-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5748, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5749, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6070", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5749", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebd88d6c-aa10-482c-aa3f-cd254a125efe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5748, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005748__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5749-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5749, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5750, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6071", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5750", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1849e78e-48be-40d1-8dc8-d295d01c4f9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 498971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5749, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005749__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5750-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5750, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5751, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6072", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5751", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72eeb010-eb1a-430a-91a7-2a62fe409ff7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5750, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005750__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5751-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5751, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "498921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5752, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6073", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5752", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b221d0a-815a-4592-a31c-e500d79402d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5751, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005751__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5752-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5752, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5753, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6074", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5753", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a71945f-f914-4b47-92d8-64df4ce1c3c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5752, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005752__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5753-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5753, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5754, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6075", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5754", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "284fa620-c0e8-42c9-8929-48b14195ac91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5753, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005753__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5754-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5754, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5755, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6076", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5755", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e7f06518-51ba-46f2-a0fa-1e17cbb27929", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5754, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005754__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5755-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5755, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5756, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6077", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5756", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb141fb0-5720-4770-ab6a-1eaf5e8f2de1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5755, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005755__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5756-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5756, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5757, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6078", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5757", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74b62a3a-469c-4793-9c54-e151c2b078f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5756, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005756__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5757-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5757, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5758, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6079", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5758", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b42f223-2a80-4521-baad-cd292c0a5468", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5757, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005757__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5758-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5758, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5759, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6080", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5759", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "990d1bde-e6df-4d4e-9d2f-743b222b4f0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5758, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005758__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5759-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5759, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5760, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6081", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5760", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1aa9a02-d339-4040-8694-ac94dd058020", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5759, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005759__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5760-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5760, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5761, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6082", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5761", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3c81d6c-8930-49b1-833f-7517d96291df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 499924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5760, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005760__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5761-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5761, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5762, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6083", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5762", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "220cc529-ce55-4b1b-9f00-a3ee555d7d86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5761, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005761__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5762-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5762, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5763, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6084", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5763", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b817ec5b-22b7-49ff-85d9-6fcbe4eb85ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5762, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005762__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5763-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5763, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "499953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5764, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6085", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5764", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6223ba58-fcb1-4a8f-a782-194d8ff389f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5763, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005763__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5764-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5764, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5765, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6086", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5765", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75e64230-7b84-4ceb-9f15-04b84ad3deb8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5764, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005764__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5765-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5765, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5766, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6087", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5766", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3113e567-5df9-4787-bf4b-c154c7260af7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5765, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005765__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5766-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5766, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5767, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6088", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5767", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "add45e8f-f132-47d3-9e96-dd65a9e48915", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5766, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005766__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5767-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5767, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5768, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6089", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5768", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3eb85d14-da72-4d0c-92a9-1c51a22fe00c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5767, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005767__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5768-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5768, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5769, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6090", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5769", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23b442cc-78e0-4a02-b3d3-be8eb847ed31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5768, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005768__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5769-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5769, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5770, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6091", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5770", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86e17010-f79c-4219-a048-b9b1b8f2c1e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5769, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005769__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5770-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5770, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5771, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6092", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5771", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd98a7cc-35b1-4d73-8de6-7f5101b22b0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5770, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005770__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5771-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5771, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5772, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6093", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5772", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d45efc6d-95a7-44a1-aae2-38ea2d424bfa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5771, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005771__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5772-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5772, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5773, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6094", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5773", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6564465-2d7a-410c-81e1-24ea1c723efd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 500955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5772, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005772__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5773-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5773, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5774, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6095", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5774", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "edb22208-4704-4016-b00b-43fcde0de149", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5773, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005773__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5774-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5774, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "500906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5775, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6096", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5775", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b822f10-f9ea-4e9e-a129-bbd8d1b8b142", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5774, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005774__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5775-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5775, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5776, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6097", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5776", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7132485b-8553-4f0a-a273-387b573141a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5775, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005775__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5776-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5776, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5777, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6098", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5777", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d827deca-6180-45eb-abce-2c7f8c1b0fac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5776, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005776__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5777-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5777, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5778, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6099", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5778", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a8d63757-c26b-4e3f-a124-c80823d67f52", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5777, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005777__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5778-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5778, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5779, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6100", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5779", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c272fa80-2338-4d90-993d-771e6ee07865", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5778, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005778__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5779-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5779, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5780, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6101", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5780", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f32308e-3d75-4fa4-b421-2a34ea65757e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5779, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005779__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5780-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5780, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5781, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6102", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5781", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2b59af8-371d-4784-b74a-5c4516c58958", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5780, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005780__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5781-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5781, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5782, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6103", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5782", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a201b53-344a-4b72-aab4-4ef18be16037", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5781, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005781__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5782-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5782, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5783, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6104", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5783", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "546f0c2a-a3e7-49a3-a72c-bf9b69342dbf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5782, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005782__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5783-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5783, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5784, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6105", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5784", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c562ddc2-2a24-4d1c-8445-308559daa378", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 501908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5783, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005783__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5784-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5784, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5785, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6106", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5785", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4c7d580-3b49-4c28-b200-373ff2e7ec01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5784, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005784__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5785-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5785, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5786, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6107", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5786", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c30c48e6-ad6a-4037-ac8d-261dac9bf9d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5785, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005785__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5786-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5786, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "501953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5787, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6108", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5787", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5347bee8-fe00-44ca-8553-52e0c9a73169", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5786, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005786__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5787-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5787, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5788, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6109", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5788", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c452f7cd-7481-48af-a866-c81b4423e9d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5787, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005787__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5788-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5788, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5789, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6110", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5789", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f14ff4ad-f015-4db0-9f2a-e2d8a9ec125a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5788, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005788__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5789-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5789, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5790, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6111", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5790", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a44cb87-d34d-460e-ae42-ba6dd93a0bd7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5789, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005789__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5790-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5790, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5791, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6112", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5791", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71b52761-53ee-432f-9202-15f699051e6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5790, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005790__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5791-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5791, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5792, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6113", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5792", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f08b6ecd-0dd5-4d0a-af4c-998484c57f99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5791, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005791__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5792-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5792, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5793, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6114", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5793", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33eb78b3-5453-402b-8f4a-623d2af4be4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5792, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005792__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5793-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5793, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5794, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6115", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5794", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf186bdb-87fb-46f4-a198-714d216afeab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5793, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005793__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5794-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5794, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5795, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6116", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5795", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ced924f9-496f-4d62-adc8-d7e6cbe3cf34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5794, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005794__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5795-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5795, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5796, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6117", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5796", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c183be5-8776-4852-9ce2-02ba2d91f87f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 502955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5795, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005795__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5796-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5796, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5797, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6118", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5797", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "146779ab-4ccf-4823-9cf9-5a6028d9e963", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5796, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005796__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5797-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5797, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "502906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5798, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6119", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5798", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a53a8a40-3794-4a32-9a46-27ce5dcc1e97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5797, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005797__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5798-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5798, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5799, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6120", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5799", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97a55ec0-8944-4930-bf5b-d6aff31b7bd2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5798, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005798__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5799-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5799, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5800, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6121", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5800", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1eaa50a-9bfa-4a66-943c-ca918160c4f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5799, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005799__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5800-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5800, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5801, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6122", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5801", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95882d14-74bf-4506-9faf-0cd1c075f4db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5800, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005800__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5801-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5801, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5802, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6123", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5802", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51ed9521-e82c-4c7e-b515-aada769eb970", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5801, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005801__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5802-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5802, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5803, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6124", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5803", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0bb48ed8-c924-46ef-bc22-094bd5fab327", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5802, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005802__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5803-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5803, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5804, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6125", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5804", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "317ec7e0-0681-4179-abbb-1a5e3a66742c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5803, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005803__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5804-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5804, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5805, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6126", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5805", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9135955a-e56c-447c-b73c-2ce2c9df8f11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5804, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005804__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5805-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5805, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5806, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6127", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5806", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57fa575d-33aa-4828-b0e4-933e2f2e1b2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5805, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005805__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5806-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5806, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5807, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6128", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5807", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f8f47ce-b313-49a1-8a86-7a86006ab502", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 503908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5806, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005806__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5807-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5807, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5808, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6129", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5808", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0d694b6-4a20-4993-9313-9e0f3cbdbe54", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5807, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005807__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5808-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5808, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5809, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6130", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5809", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf1c52e6-47d1-430c-821f-9d295f7a35b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5808, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005808__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5809-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5809, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "503953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5810, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6131", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5810", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bca5acfc-08a1-4230-8a39-00648398413b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5809, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005809__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5810-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5810, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5811, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6132", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5811", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53cd26c5-8484-4d06-b729-100fd6b949fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5810, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005810__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5811-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5811, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5812, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6133", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5812", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "996df5df-c9c0-4af7-8b14-70d72d45ea9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5811, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005811__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5812-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5812, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5813, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6134", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5813", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4e141ed-6031-4feb-b3e0-1a345d97e5df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5812, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005812__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5813-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5813, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5814, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6135", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5814", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a647800-d116-4247-9c77-d50cf7e42a8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5813, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005813__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5814-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5814, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5815, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6136", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5815", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47735348-ce7f-493f-939e-0885c04f2707", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5814, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005814__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5815-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5815, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5816, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6137", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5816", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39aa8989-1204-40ce-960f-734b30f88999", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5815, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005815__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5816-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5816, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5817, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6138", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5817", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a0ad7aa-771b-4e03-ab2e-67a04fb5a362", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5816, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005816__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5817-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5817, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5818, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6139", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5818", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7867af94-8615-4c52-a41c-e0dca330adc2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5817, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005817__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5818-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5818, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5819, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6140", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5819", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd379543-f036-45df-bbcf-9372d230b50e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 504955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5818, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005818__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5819-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5819, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5820, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6141", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5820", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2489ff65-8548-4322-93eb-383d539ff30f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5819, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005819__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5820-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5820, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5821, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6142", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5821", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "283d3d60-2a13-4abb-acf1-1f61c339c03c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5820, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005820__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5821-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5821, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "504984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5822, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6143", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5822", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e3b4198-03fa-4fe3-ae46-798469ef7339", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5821, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005821__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5822-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5822, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5823, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6144", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5823", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3742be84-8563-4c92-b74c-89a8ffb3486f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5822, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005822__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5823-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5823, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5824, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6145", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5824", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e915ff43-b58d-43b1-8bbf-a85abed7237a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5823, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005823__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5824-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5824, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5825, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6146", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5825", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1697818a-7464-4a85-8f40-bab3ae99763d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5824, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005824__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5825-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5825, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5826, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6147", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5826", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "150c02a6-c873-4f6b-994c-993c80d3b7b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5825, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005825__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5826-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5826, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5827, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6148", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5827", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea381da2-bda7-498b-8dcf-e79dc0f16af6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5826, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005826__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5827-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5827, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5828, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6149", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5828", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdec0b15-6485-451f-af26-a0bd306806fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5827, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005827__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5828-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5828, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5829, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6150", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5829", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e83a1870-b5a1-4915-990a-66aa73fe078a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5828, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005828__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5829-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5829, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5830, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6151", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5830", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "868df14d-1654-4042-813e-67449d1d18e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5829, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005829__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5830-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5830, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5831, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6152", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5831", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "124d4142-519e-4527-8040-3ba70d664b88", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 505986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5830, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005830__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5831-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5831, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5832, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6153", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5832", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5254a734-876e-41f0-ac78-533a1cb42706", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5831, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005831__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5832-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5832, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "505937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5833, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6154", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5833", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c81d53ad-5049-4adf-93db-8e4f45b6c0ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5832, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005832__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5833-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5833, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5834, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6155", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5834", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be37438e-605a-46cc-aaf5-1e4b48334830", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5833, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005833__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5834-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5834, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5835, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6156", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5835", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a46fe68-ab3b-4c89-aeb1-d1becef3a3d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5834, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005834__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5835-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5835, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5836, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6157", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5836", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e49b9265-7974-4b0b-84bb-e88dd8e342fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5835, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005835__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5836-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5836, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5837, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6158", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5837", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e68d073-ba26-4ce5-8db0-052ee493eea8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5836, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005836__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5837-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5837, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5838, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6159", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5838", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4612f807-6ce0-404f-af63-9e7778da722f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5837, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005837__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5838-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5838, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5839, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6160", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5839", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c07c71d8-78fe-4fe6-a8f3-95df398fcf36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5838, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005838__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5839-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5839, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5840, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6161", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5840", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88370819-7a11-4417-8701-d444ad470a4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5839, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005839__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5840-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5840, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5841, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6162", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5841", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67bd28ef-6fbc-4287-80b0-0c7e65f173c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5840, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005840__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5841-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5841, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5842, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6163", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5842", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "128e6236-b1ee-460e-8628-069894599c08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 506940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5841, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005841__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5842-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5842, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5843, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6164", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5843", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcf6c5c4-ef72-48d3-8ce8-8c0b8e7df5f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5842, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005842__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5843-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5843, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5844, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6165", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5844", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74efd6ba-9006-467e-8dab-607c7810c450", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5843, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005843__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5844-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5844, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "506984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5845, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6166", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5845", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "079cfa80-7c0e-421c-875b-988dde2bc418", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5844, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005844__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5845-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5845, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5846, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6167", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5846", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a7c4f12-00ca-4694-bead-0a9db86bdf40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5845, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005845__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5846-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5846, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5847, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6168", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5847", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9578ae89-6684-449e-91b7-2fc0fbfd81c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5846, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005846__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5847-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5847, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5848, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6169", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5848", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "252f349e-522a-42d0-979d-79977c46e84f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5847, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005847__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5848-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5848, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5849, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6170", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5849", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45c7de67-b7e9-4b7c-989c-757e1ce51652", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5848, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005848__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5849-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5849, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5850, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6171", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5850", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8db7d079-c9da-4244-848c-c829c26c1f75", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5849, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005849__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5850-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5850, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5851, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6172", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5851", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a90f6d42-2d9f-4d8e-9af3-b393ebe75164", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5850, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005850__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5851-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5851, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5852, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6173", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5852", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfc9cb54-fcae-4dc7-a92f-aa1c60bf087c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5851, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005851__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5852-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5852, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5853, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6174", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5853", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac38fbd1-f275-4261-9d16-5210648605f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5852, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005852__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5853-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5853, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5854, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6175", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5854", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58fb32d5-e678-4b5f-b361-556262e77e93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 507986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5853, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005853__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5854-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5854, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5855, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6176", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5855", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91e87e67-b70f-4dc3-810d-a0572b196d2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5854, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005854__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5855-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5855, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "507937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5856, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6177", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5856", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4927f13f-aac6-4146-9377-25454da5a5e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5855, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005855__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5856-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5856, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5857, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6178", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5857", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17288632-7c56-422c-914b-82b644f66e80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5856, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005856__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5857-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5857, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5858, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6179", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5858", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "05efd761-a1a6-4a59-8470-02610c6d1de2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5857, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005857__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5858-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5858, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5859, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6180", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5859", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f6b5456-c037-42c8-95e5-a9c19291ef1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5858, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005858__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5859-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5859, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5860, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6181", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5860", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fc27372-9014-4cc6-8207-a55b83fc0fe3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5859, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005859__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5860-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5860, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5861, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6182", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5861", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f92fd2d-8fa9-4c80-8ea8-3285d5038097", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5860, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005860__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5861-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5861, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5862, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6183", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5862", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57d6348e-01ee-409a-a68c-d072033a6b0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5861, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005861__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5862-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5862, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5863, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6184", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5863", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4b4014d-bbef-4f88-9fa6-7e8179278b97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5862, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005862__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5863-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5863, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5864, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6185", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5864", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8300b4b6-4c5e-4458-90d6-508a89f57464", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5863, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005863__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5864-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5864, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5865, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6186", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5865", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfd39641-3928-4559-8d30-83a5a64c2487", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 508940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5864, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005864__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5865-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5865, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5866, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6187", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5866", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6d056a3-2648-43fa-bfce-af44d5a3cb7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5865, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005865__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5866-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5866, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5867, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6188", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5867", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aedd7e19-9783-4603-a722-6c9b2da4e47d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5866, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005866__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5867-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5867, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "508984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5868, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6189", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5868", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf8ced62-81af-403d-bf70-79d53adccbf6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5867, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005867__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5868-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5868, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5869, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6190", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5869", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "989207d1-b102-4904-a519-91e20a19f675", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5868, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005868__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5869-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5869, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5870, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6191", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5870", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa560590-3a2a-4826-8a62-70853d3bed0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5869, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005869__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5870-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5870, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5871, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6192", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5871", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d30fc8d-0ce3-4818-b40e-ccfea591f43b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5870, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005870__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5871-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5871, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5872, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6193", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5872", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33e2b20f-a883-4f88-b0d1-9e44b4389620", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5871, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005871__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5872-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5872, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5873, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6194", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5873", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2679590e-3fc3-448c-85f6-5b778c52910b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5872, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005872__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5873-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5873, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5874, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6195", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5874", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fe1c557-17f5-470b-bdc2-88496fb9e0de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5873, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005873__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5874-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5874, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5875, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6196", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5875", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b36607fb-931e-4961-ad55-84002bb655fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5874, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005874__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5875-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5875, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5876, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6197", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5876", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9ed3ec0-a303-4ed7-9f92-a6d1668c83fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5875, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005875__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5876-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5876, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5877, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6198", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5877", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7952e64e-5ffa-45ec-9ab5-ed2f6fcd606b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 509986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5876, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005876__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5877-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5877, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5878, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6199", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5878", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0f8ee01-e302-46d8-a673-2f4880e99606", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5877, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005877__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5878-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5878, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "509937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5879, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6200", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5879", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72fa2bfd-99b4-4eae-aca6-9ac8add5c89b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5878, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005878__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5879-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5879, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5880, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6201", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5880", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0830929a-1670-42bf-82c7-06de2b7c3252", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5879, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005879__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5880-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5880, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5881, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6202", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5881", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab3aa811-dd17-4bc3-b1fb-d76e04ffe919", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5880, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005880__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5881-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5881, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5882, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6203", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5882", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "508488df-60e6-477f-9b62-a594e6441004", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5881, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005881__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5882-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5882, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5883, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6204", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5883", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa55add4-f619-4f97-b1e0-ee899a64e8b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5882, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005882__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5883-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5883, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5884, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6205", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5884", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efee7c93-e798-417f-8e00-259239cfeac1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5883, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005883__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5884-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5884, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5885, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6206", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5885", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e44482e-2d08-4bc0-ac08-fb2f45fe7d31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5884, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005884__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5885-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5885, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5886, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6207", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5886", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5f540fc-260c-4182-acb1-35cf3e26d499", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5885, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005885__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5886-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5886, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5887, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6208", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5887", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "042457cd-1d48-4c1a-82d0-c37e7358e02c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5886, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005886__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5887-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5887, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5888, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6209", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5888", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "596e4036-541f-4c67-af09-5f7515ead131", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 510940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5887, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005887__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5888-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5888, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5889, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6210", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5889", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "172a3b00-3c81-47b9-af69-8ef9e35541e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5888, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005888__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5889-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5889, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5890, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6211", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5890", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94bf1e03-12f1-4717-a126-7db58c3e5606", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5889, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005889__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5890-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5890, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "510968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5891, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6212", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5891", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "148ea00e-2316-4f6d-b766-a1547fee67c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5890, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005890__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5891-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5891, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5892, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6213", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5892", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de1eaba8-c320-4587-9678-e5da1b81322c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5891, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005891__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5892-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5892, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5893, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6214", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5893", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46528b61-a854-4be0-946b-e9d4da92f346", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5892, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005892__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5893-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5893, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5894, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6215", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5894", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c2c7819-6800-40ca-bcb1-e3828e8c170a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5893, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005893__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5894-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5894, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5895, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6216", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5895", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11013354-9d4d-40f7-8701-a89fc939333e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5894, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005894__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5895-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5895, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5896, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6217", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5896", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7883afb3-44e4-4927-8709-5bb36e2013ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5895, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005895__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5896-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5896, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5897, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6218", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5897", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c690e692-a459-42c6-8a29-06c3c247412d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5896, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005896__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5897-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5897, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5898, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6219", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5898", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70dd5e04-36da-4504-b835-0f8571a7fd97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5897, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005897__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5898-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5898, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5899, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6220", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5899", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a8029f8-02ef-4db6-906f-1912e6d7f7c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5898, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005898__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5899-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5899, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5900, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6221", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5900", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc0bae16-722b-4bef-88dd-964e109b87f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 511971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5899, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005899__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5900-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5900, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5901, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6222", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5901", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a444f7c-fdc2-4cfc-8ac7-8a62fd90dffc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5900, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005900__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5901-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5901, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "511937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5902, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6223", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5902", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bda8438a-d653-48b3-a5a2-9eb61ab53f78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5901, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005901__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5902-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5902, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5903, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6224", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5903", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8468d124-76a1-4035-a9ea-8cb4136318bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5902, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005902__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5903-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5903, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5904, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6225", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5904", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "781dff25-e5dc-4940-9b33-e018ab9249c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5903, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005903__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5904-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5904, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5905, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6226", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5905", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29307a51-a680-4116-90ff-3b2e8d787ea7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5904, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005904__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5905-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5905, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5906, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6227", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5906", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6200b93-7363-41e9-af43-7c97dc5d4cf8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5905, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005905__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5906-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5906, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5907, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6228", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5907", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e58e949b-0340-4d0e-bb97-466b64a60e63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5906, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005906__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5907-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5907, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5908, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6229", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5908", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7bb9373-7f37-48c8-9d5d-418e3de4c8a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5907, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005907__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5908-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5908, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5909, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6230", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5909", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e6818b7-5ec7-45a7-958c-51684c49324e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5908, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005908__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5909-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5909, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5910, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6231", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5910", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08005ced-5238-45e6-9f4f-2ad7b1df7ef9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5909, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005909__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5910-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5910, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5911, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6232", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5911", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "943b4e3f-4a81-4325-ba3b-1b0517d6f7b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 512924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5910, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005910__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5911-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5911, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5912, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6233", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5912", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "628068da-f610-4edc-a755-dd1e76e1d5e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5911, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005911__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5912-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5912, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5913, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6234", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5913", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a3dbf12a-a0da-4d1b-bf86-ae1f2487b818", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5912, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005912__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5913-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5913, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "512968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5914, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6235", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5914", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a612bce-f51e-4802-aac5-302dc84cccca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5913, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005913__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5914-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5914, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5915, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6236", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5915", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47917098-0010-464e-9959-8d2fb8eb1a05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5914, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005914__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5915-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5915, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5916, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6237", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5916", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bb2394b-7ad5-47b7-b860-808fb0b2f059", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5915, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005915__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5916-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5916, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5917, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6238", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5917", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65480d9a-ad08-4eb4-bb1c-c39613c3c871", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5916, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005916__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5917-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5917, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5918, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6239", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5918", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d166e23-8b74-4f20-8360-5a15b95c7d28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5917, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005917__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5918-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5918, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5919, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6240", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5919", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a17eb413-2334-458f-be4f-876d230e3401", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5918, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005918__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5919-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5919, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5920, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6241", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5920", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "784f6e2c-f6ae-4f38-be46-4cde3773ef7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5919, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005919__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5920-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5920, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5921, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6242", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5921", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0fd2f8d-1076-4b98-ae91-3c281d33f69e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5920, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005920__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5921-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5921, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5922, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6243", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5922", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8071f89e-feac-4848-ac05-294faf3623d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5921, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005921__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5922-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5922, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5923, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6244", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5923", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "049864fd-dc04-4dcd-bce3-aee3cc62f65d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 513971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5922, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005922__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5923-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5923, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5924, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6245", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5924", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bca9c712-107c-4e1e-8710-fb389dfc0039", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5923, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005923__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5924-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5924, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "513921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5925, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6246", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5925", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91f5e852-59f6-41d2-9582-718c10a01ac2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5924, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005924__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5925-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5925, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5926, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6247", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5926", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2aa2703e-a189-45bc-ad4f-9d781f18b5ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5925, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005925__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5926-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5926, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5927, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6248", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5927", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e92d45d5-9a59-4cff-89da-5ad8baba98f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5926, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005926__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5927-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5927, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5928, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6249", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5928", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a254fa83-5e4a-4e26-a250-f7edd69275e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5927, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005927__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5928-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5928, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5929, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6250", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5929", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2852bf03-eb81-4380-a82c-327185e69131", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5928, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005928__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5929-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5929, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5930, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6251", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5930", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebe3430a-61b8-42a8-a1e5-ac7fe9dcffcc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5929, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005929__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5930-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5930, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5931, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6252", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5931", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d642713-f201-4f62-91c5-c14a8ab5139f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5930, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005930__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5931-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5931, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5932, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6253", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5932", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3dc7e408-310d-442f-abe5-3aee2bac6e21", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5931, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005931__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5932-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5932, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5933, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6254", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5933", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ddb7b96-bf23-4b2e-914c-bedf3a9a5749", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5932, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005932__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5933-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5933, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5934, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6255", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5934", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88360f9c-d116-4bfa-b772-e9943079ac5f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 514924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5933, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005933__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5934-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5934, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5935, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6256", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5935", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9167e730-f6ed-4f3d-b7b7-1b94d70b5f04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5934, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005934__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5935-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5935, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5936, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6257", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5936", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9cd3122d-7268-4abd-8155-6d837febcba4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5935, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005935__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5936-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5936, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "514968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5937, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6258", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5937", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db36b17e-82fb-4b42-b6fc-746786a25f17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5936, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005936__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5937-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5937, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5938, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6259", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5938", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "588511ea-c6b0-4301-afd9-91a0b8afd8f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5937, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005937__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5938-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5938, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5939, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6260", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5939", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4926d72-911d-4646-ba38-9f8c2a7ee52f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5938, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005938__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5939-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5939, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5940, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6261", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5940", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "febb4025-047f-4f99-b373-eebf56b0347d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5939, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005939__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5940-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5940, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5941, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6262", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5941", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd4e73d4-a6d4-4994-bd30-50c7e9587ae6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5940, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005940__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5941-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5941, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5942, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6263", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5942", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dcf80b61-6710-4d97-ac1b-0de2d5897674", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5941, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005941__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5942-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5942, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5943, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6264", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5943", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ae74c8d-de93-4761-9ff9-2f669bd9f62c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5942, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005942__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5943-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5943, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5944, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6265", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5944", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef399fe1-f600-482b-95c4-a9de18b276bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5943, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005943__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5944-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5944, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5945, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6266", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5945", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd7c15f6-2e02-4128-b5a5-6583b46d7bfd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5944, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005944__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5945-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5945, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5946, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6267", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5946", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "113ad61a-c4db-4990-a519-6a9391a0a4a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 515971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5945, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005945__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5946-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5946, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5947, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6268", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5947", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4926a38-2622-46b2-b1ee-dc2d14e593ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5946, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005946__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5947-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5947, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "515921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5948, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6269", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5948", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bb11059-f4e8-4e8d-b728-7055f1b04e98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5947, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005947__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5948-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5948, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5949, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6270", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5949", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f19da3d-8d88-4a19-8d7e-137de1647fd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5948, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005948__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5949-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5949, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5950, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6271", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5950", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ecc8102-26ec-43ca-9e21-3d05f2e697f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5949, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005949__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5950-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5950, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5951, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6272", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5951", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36ad2144-9d6f-480d-92ef-7c6d8772d941", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5950, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005950__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5951-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5951, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5952, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6273", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5952", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "423b08c3-7144-4b5a-8af2-fc7ca01b50d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5951, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005951__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5952-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5952, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5953, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6274", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5953", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d9be3a1-9aec-4b0b-9bc1-6a2b8dc65746", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5952, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005952__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5953-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5953, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5954, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6275", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5954", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea51d2f2-7146-4bf0-a4e5-316cce2d86cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5953, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005953__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5954-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5954, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5955, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6276", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5955", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55f91c72-014f-4c9a-86d3-9e7c514cb02c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5954, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005954__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5955-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5955, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5956, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6277", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5956", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e983d625-b3aa-4e19-9a51-3cdba0e43ae4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5955, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005955__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5956-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5956, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5957, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6278", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5957", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d35b718-4e57-46c4-bf43-12f57ef2c450", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 516924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5956, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005956__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5957-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5957, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5958, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6279", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5958", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0d1ad442-5b46-4342-abd0-2aef9caad3ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5957, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005957__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5958-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5958, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5959, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6280", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5959", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a466acb3-b393-4158-9b8a-e32a69688786", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5958, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005958__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5959-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5959, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "516953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5960, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6281", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5960", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2101ffef-ff67-4827-a1dc-87d965c870c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5959, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005959__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5960-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5960, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5961, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6282", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5961", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4f745af-cf62-4a49-9ccc-be33180ff0d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5960, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005960__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5961-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5961, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5962, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6283", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5962", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cb183cd-7e25-4f5c-81f6-7a4fe685d58b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5961, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005961__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5962-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5962, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5963, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6284", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5963", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3732c53a-d27c-4881-b5d7-6e4516124fd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5962, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005962__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5963-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5963, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5964, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6285", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5964", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38aaa8e4-4a7f-4d60-a586-c351962e14ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5963, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005963__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5964-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5964, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5965, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6286", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5965", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "33509c34-37da-4833-9135-987f15773f61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5964, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005964__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5965-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5965, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5966, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6287", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5966", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c764bc0c-b509-49ff-b32d-64907ae8b1a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5965, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005965__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5966-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5966, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5967, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6288", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5967", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4eb979c-5ba4-4e5f-85b8-06abec37e67c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5966, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005966__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5967-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5967, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5968, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6289", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5968", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61b4fe02-1801-4bfe-915a-b815aef396de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5967, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005967__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5968-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5968, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5969, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6290", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5969", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14ca2ffd-dac4-4475-afd2-8a0a29782289", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 517955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5968, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005968__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5969-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5969, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5970, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6291", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5970", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e60e1b7-0c47-4b3b-ad08-4002916939cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5969, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005969__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5970-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5970, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "517921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5971, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6292", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5971", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d20ae670-1ebc-483e-83da-c6eb3a838cb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5970, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005970__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5971-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5971, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5972, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6293", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5972", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58cdfb5c-21fd-4d32-8cdf-33d17b1e9f40", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5971, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005971__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5972-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5972, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5973, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6294", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5973", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6439072-f078-4e21-a226-3699e94e89ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5972, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005972__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5973-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5973, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5974, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6295", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5974", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "137662bc-2f6b-4d4b-93fd-476758d44e96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5973, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005973__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5974-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5974, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5975, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6296", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5975", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a55d6c82-83e1-48fa-9eae-2adbdacf1400", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5974, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005974__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5975-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5975, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5976, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6297", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5976", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d67314f-07cd-4e68-849b-96d30147c140", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5975, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005975__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5976-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5976, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5977, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6298", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5977", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "623ed131-c494-43ae-857e-a53b80b36126", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5976, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005976__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5977-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5977, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5978, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6299", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5978", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "502342d9-cf34-4163-b75a-1be68b575dc6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5977, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005977__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5978-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5978, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5979, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6300", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5979", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94adcfda-9c7d-435b-bdc8-98dc943cd8f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5978, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005978__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5979-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5979, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5980, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6301", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5980", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84cc00e4-1614-4a36-b443-650674ca1675", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 518908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5979, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005979__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5980-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5980, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5981, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6302", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5981", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8b9b8a9-ee20-467a-b295-51dc2f89f196", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5980, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005980__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5981-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5981, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5982, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6303", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5982", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2480e311-5f33-4b4e-9a4a-9e0359661e6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5981, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005981__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5982-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5982, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "518953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5983, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6304", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5983", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d02f3d92-41a6-4c47-84dc-24dec6d1ef17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5982, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005982__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5983-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5983, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5984, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6305", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5984", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd519180-3b74-4bd2-a23f-896813ce0e6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5983, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005983__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5984-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5984, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5985, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6306", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5985", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "26780999-1fd4-4466-b899-500750390a4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5984, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005984__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5985-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5985, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5986, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6307", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5986", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cfc9c0c-fec9-4c90-8977-60f1a3d861a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5985, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005985__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5986-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5986, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5987, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6308", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5987", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fcd261a-c8ba-4acf-afa3-3bb6738844f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5986, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005986__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5987-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5987, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5988, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6309", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5988", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bb1c0d2-9f33-4041-af5e-b83ecf203c45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5987, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005987__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5988-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5988, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5989, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6310", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5989", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "059c0f20-856f-4ea0-b837-5ca180c1b620", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5988, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005988__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5989-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5989, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5990, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6311", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5990", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f384fe4-5014-4bbf-9060-a248c065db0e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5989, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005989__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5990-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5990, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5991, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6312", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5991", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7996b6f9-b875-46fc-808a-015c17afeccf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5990, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005990__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5991-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5991, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5992, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6313", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5992", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "532b7479-49a3-4817-bba5-26b47c1cf77f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 519955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5991, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005991__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5992-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5992, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5993, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6314", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5993", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "146fc336-c346-4bc1-a86a-6ee8ddbbe646", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5992, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005992__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5993-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5993, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "519906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5994, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6315", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5994", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d715583b-f9f7-41d4-9bc8-496144c09746", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5993, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005993__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5994-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5994, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5995, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6316", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5995", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ce9d34b-5946-45b3-9691-633a5ef5a3b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5994, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005994__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5995-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5995, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5996, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6317", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5996", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7741f9bd-612d-4006-96a0-9757c7b88ae3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5995, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005995__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5996-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5996, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5997, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6318", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5997", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36077550-d75f-45d5-ae3c-ca06761c3879", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5996, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005996__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5997-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5997, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5998, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6319", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5998", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d89bf637-2faf-46b1-8833-40138f222dbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5997, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005997__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5998-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5998, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 5999, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6320", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "5999", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a15ba50f-cdb8-4a9d-b37c-d5c1b189d773", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5998, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005998__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-5999-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 5999, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6000, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6321", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6000", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28fd354c-734f-40be-93a8-50b0f56bafd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 5999, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000005999__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6000-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6000, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6001, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6322", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6001", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f050fca9-93f5-437e-b271-e1e02a04c622", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6000, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6001-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6001, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6002, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6323", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6002", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff25e7b2-520d-4214-aaee-03b7bc1a5431", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6001, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006001__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6002-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6002, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6003, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6324", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6003", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57bf78c9-73f5-4392-94db-8734f65ca38d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 520908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6002, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006002__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6003-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6003, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6004, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6325", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6004", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29202822-a99d-430b-91ca-3a2edaa0b5c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6003, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006003__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6004-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6004, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6005, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6326", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6005", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a0434a4-9e91-4192-bd28-df5f1ab99b77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6004, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006004__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6005-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6005, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "520953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6006, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6327", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6006", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18ea9c01-6c3a-4db8-8672-59e1db42d35c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6005, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006005__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6006-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6006, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6007, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6328", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6007", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8133a462-a713-44e6-8af6-94186f9a909d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6006, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006006__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6007-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6007, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6008, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6329", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6008", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38b551a7-7207-480a-aa29-75d2250f1d0d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6007, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006007__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6008-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6008, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6009, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6330", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6009", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "054eca2d-d481-4af4-878a-46ad61fd1afb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6008, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006008__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6009-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6009, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6010, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6331", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6010", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30ebe5ab-e219-4b70-a309-232e5ad3f7b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6009, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006009__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6010-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6010, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6011, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6332", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6011", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61472a18-fc17-4ff6-98c5-d3257c66663b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6010, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006010__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6011-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6011, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6012, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6333", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6012", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbf0325d-08e6-4c9a-9150-ec2f4bf4ed13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6011, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006011__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6012-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6012, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6013, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6334", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6013", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c5345c1-97e2-4c1a-824a-5c067b303054", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6012, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006012__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6013-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6013, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6014, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6335", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6014", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8775fdc-ef52-443b-85d6-abb23ef489f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6013, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006013__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6014-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6014, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6015, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6336", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6015", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc63929f-624c-4b4e-bbb8-23b76ea9ec11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 521955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6014, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006014__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6015-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6015, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6016, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6337", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6016", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcb5e145-9cc2-44f4-8c43-7f2599b4b5f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6015, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006015__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6016-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6016, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6017, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6338", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6017", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4462d17b-3e7d-4b15-8fef-d9ceac9922e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6016, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006016__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6017-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6017, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "521984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6018, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6339", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6018", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "618d5aa0-7eb3-42a5-9026-72493ad57057", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6017, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006017__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6018-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6018, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6019, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6340", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6019", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "752c8b31-494a-42fc-899a-2e72210eec25", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6018, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006018__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6019-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6019, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6020, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6341", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6020", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccc18732-4c64-44bc-9c95-38997b5103d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6019, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006019__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6020-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6020, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6021, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6342", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6021", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0bca2719-95f0-47bb-a11c-81ecc2c9c6a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6020, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006020__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6021-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6021, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6022, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6343", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6022", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18d0a1ac-6bde-43a4-9587-a19152c4236e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6021, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006021__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6022-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6022, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6023, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6344", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6023", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66d9f8ee-1352-4d2e-96b2-e0c48e16ce39", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6022, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006022__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6023-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6023, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6024, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6345", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6024", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9aa4f954-71a2-4456-a89c-21edc78d5af4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6023, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006023__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6024-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6024, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6025, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6346", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6025", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a68bc5e-6e4e-4655-9004-1af4c8279f02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6024, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006024__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6025-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6025, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6026, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6347", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6026", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b2b43e4-8130-43bb-9bfb-a67e5eb919b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6025, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006025__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6026-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6026, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6027, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6348", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6027", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "593be3ff-23a4-4c16-b9ec-9d9647d83e85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 522986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6026, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006026__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6027-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6027, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6028, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6349", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6028", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2d6f429-3705-4e75-b425-6f3c786bb9f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6027, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006027__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6028-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6028, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "522937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6029, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6350", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6029", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0101ca98-d0fa-4fc6-9043-30f7d09fd7e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6028, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006028__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6029-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6029, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6030, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6351", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6030", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4acd2d8-d02a-44ea-85c8-18af61e9cc01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6029, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006029__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6030-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6030, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6031, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6352", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6031", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4ed0af2-cdab-4192-aaa2-5ac864e17de3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6030, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006030__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6031-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6031, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6032, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6353", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6032", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0bb6663-9990-4399-92a0-5b740d076ecd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6031, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006031__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6032-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6032, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6033, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6354", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6033", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fd675c5-c2f6-4d30-b2a3-53fefaf95ec2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6032, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006032__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6033-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6033, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6034, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6355", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6034", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2ec8945-46ee-4909-b86c-f88a9f764753", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6033, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006033__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6034-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6034, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6035, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6356", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6035", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e922a45c-f54c-49c6-b755-1a7d5d4746a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6034, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006034__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6035-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6035, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6036, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6357", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6036", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7374f16c-e3b0-4c48-b71d-99c228e7a336", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6035, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006035__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6036-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6036, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6037, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6358", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6037", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a407ba68-4146-438d-9ecf-79e21eaa86a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6036, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006036__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6037-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6037, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6038, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6359", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6038", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff53e4a3-32d2-4444-bcb5-26f542524769", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 523940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6037, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006037__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6038-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6038, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6039, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6360", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6039", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b893d05b-7c20-4d62-bab9-0b4a73983970", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6038, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006038__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6039-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6039, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6040, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6361", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6040", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc930eb9-10f4-4f89-92e8-9cc41b4396c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6039, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006039__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6040-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6040, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "523984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6041, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6362", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6041", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3dc4610-be33-4994-a54b-48cefb996b9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6040, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006040__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6041-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6041, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6042, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6363", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6042", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3d87457-e07e-4b51-8146-deb9690302d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6041, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006041__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6042-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6042, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6043, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6364", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6043", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06cadab5-dac1-4ac0-a4ab-f60add5cae95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6042, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006042__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6043-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6043, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6044, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6365", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6044", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9273d1a7-eec5-420e-b33b-9f4ed2da74a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6043, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006043__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6044-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6044, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6045, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6366", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6045", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32e883ad-bb0a-419a-b39e-059cb468b370", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6044, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006044__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6045-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6045, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6046, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6367", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6046", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b98d0b7-b1d7-4c26-b314-60f20643739a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6045, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006045__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6046-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6046, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6047, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6368", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6047", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe09a103-cef3-4a92-8199-72c06573fe81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6046, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006046__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6047-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6047, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6048, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6369", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6048", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38e4c176-e677-47c7-a4d4-aec57419f95d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6047, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006047__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6048-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6048, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6049, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6370", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6049", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bbd8d00-2fd2-4de7-85cb-81e1340b1fe3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6048, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006048__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6049-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6049, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6050, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6371", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6050", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cd04e5f-ac1e-450b-9a2a-68f097ed135c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 524986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6049, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006049__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6050-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6050, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6051, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6372", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6051", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e4b478a-2d55-4e77-9002-a45437e0a973", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6050, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006050__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6051-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6051, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "524937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6052, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6373", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6052", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a170e780-3ca0-4550-90be-4df85c9103f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6051, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006051__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6052-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6052, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6053, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6374", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6053", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "555a687e-3852-4ac9-8e64-b2aa5bb39db2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6052, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006052__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6053-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6053, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6054, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6375", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6054", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1ac7065-56b0-4c70-a1f5-38a84074da43", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6053, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006053__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6054-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6054, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6055, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6376", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6055", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cad10b53-e7ff-42d2-a5b9-1c2dc8eb7a0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6054, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006054__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6055-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6055, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6056, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6377", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6056", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca4174c6-44c6-4d60-9924-db52827c81ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6055, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006055__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6056-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6056, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6057, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6378", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6057", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "946bd911-3a70-44c7-aa24-f8bef6635019", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6056, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006056__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6057-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6057, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6058, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6379", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6058", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8eee82c-85ed-4cc9-867e-6115d87a7ecd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6057, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006057__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6058-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6058, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6059, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6380", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6059", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b65cbb8b-cfde-4fa3-ad68-5776e81caf1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6058, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006058__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6059-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6059, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6060, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6381", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6060", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7db363f4-555d-44be-bb84-bc542dfe858f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6059, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006059__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6060-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6060, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6061, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6382", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6061", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5608b518-c8b7-4700-9a91-e8358c14e58b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 525940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6060, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006060__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6061-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6061, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6062, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6383", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6062", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d56e784-6ff3-4789-87cf-90cbc37a732c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6061, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006061__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6062-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6062, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6063, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6384", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6063", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13bea40e-0c52-474d-a23c-9de6a5347be1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6062, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006062__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6063-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6063, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "525984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6064, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6385", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6064", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae51bb33-3a25-47a8-8175-54b5778fd40d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6063, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006063__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6064-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6064, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6065, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6386", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6065", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15610293-f9f2-4097-9ad1-7ba2612b64d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6064, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006064__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6065-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6065, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6066, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6387", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6066", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb9fabd0-16e7-4598-9982-bd0de7d1d594", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6065, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006065__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6066-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6066, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6067, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6388", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6067", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91d329f7-dc73-49bf-b7a6-1c78980968ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6066, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006066__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6067-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6067, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6068, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6389", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6068", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8d8a09b-f017-43ba-b275-939b84a02bfd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6067, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006067__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6068-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6068, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6069, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6390", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6069", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af79241a-3737-49c1-bb23-cea2dae34588", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6068, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006068__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6069-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6069, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6070, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6391", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6070", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29f57674-2b52-44e7-b678-766e5662e413", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6069, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006069__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6070-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6070, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6071, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6392", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6071", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86c8c12a-37fe-400d-8eb3-c20d7833de24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6070, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006070__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6071-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6071, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6072, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6393", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6072", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5430cdf-ff7b-42b0-8a41-32bd1eb868d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6071, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006071__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6072-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6072, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6073, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6394", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6073", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ec7d4cb-272d-4bfe-97b6-801d3de19bd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 526986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6072, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006072__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6073-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6073, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6074, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6395", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6074", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa54f315-3f68-4715-9aa4-0d1ec5d7772f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6073, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006073__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6074-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6074, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "526937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6075, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6396", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6075", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b7487ac-36aa-459c-a0a0-ac5e6f4fcaa9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6074, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006074__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6075-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6075, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6076, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6397", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6076", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f370d2aa-1b08-4ba8-97d9-9c8be872307a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6075, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006075__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6076-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6076, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6077, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6398", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6077", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6344203c-ed96-438f-8084-40034473ade6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6076, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006076__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6077-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6077, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6078, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6399", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6078", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5423685b-7a5d-43b3-99c3-f113843def13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6077, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006077__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6078-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6078, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6079, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6400", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6079", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "208b3df4-b28c-4838-8b45-c4c3d32cdb1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6078, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006078__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6079-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6079, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6080, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6401", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6080", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1a83368-c55d-4580-be5a-4707fbc7b15b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6079, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006079__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6080-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6080, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6081, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6402", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6081", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7727b9d-538b-4ef1-a2a2-1f405cd28f01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6080, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006080__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6081-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6081, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6082, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6403", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6082", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cdfc37a-a97f-46ee-a35e-3021dcb3d854", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6081, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006081__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6082-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6082, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6083, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6404", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6083", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42ff6719-9513-46df-9add-f2637d04a4a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6082, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006082__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6083-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6083, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6084, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6405", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6084", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f08a70dd-677c-46d9-b895-af882d67b459", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 527940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6083, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006083__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6084-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6084, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6085, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6406", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6085", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a49f555a-e58b-4bd4-ac48-5e0bc8e76eee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6084, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006084__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6085-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6085, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6086, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6407", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6086", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2834790e-b0d6-46e2-ba89-6aef91b790c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6085, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006085__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6086-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6086, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "527968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6087, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6408", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6087", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aef21d46-257b-499c-be2c-eda0e742c91e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6086, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006086__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6087-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6087, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6088, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6409", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6088", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e216c74-1d65-458e-931e-fd8991046c76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6087, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006087__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6088-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6088, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6089, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6410", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6089", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bdd718e-acd8-4206-9d0d-ab35633c8df2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6088, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006088__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6089-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6089, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6090, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6411", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6090", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e29f8c8b-0ecc-434a-abd0-56573419e041", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6089, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006089__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6090-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6090, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6091, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6412", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6091", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9603dbe3-40a5-47f4-96a3-26b0e5b30fa7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6090, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006090__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6091-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6091, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6092, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6413", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6092", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00cc75dd-324a-4a7e-9d96-5de68412f7a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6091, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006091__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6092-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6092, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6093, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6414", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6093", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "782e8877-424f-4c9b-ace4-b497854fca6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6092, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006092__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6093-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6093, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6094, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6415", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6094", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16703c99-8cd0-410d-96f2-13f3934add30", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6093, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006093__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6094-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6094, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6095, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6416", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6095", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81a79b67-e15b-48b2-aa65-a29248c091cc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6094, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006094__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6095-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6095, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6096, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6417", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6096", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfbc9bfe-a606-49b3-8a67-ab38e6da1734", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 528971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6095, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006095__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6096-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6096, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6097, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6418", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6097", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c03d3ec0-799a-4c01-8329-db1ee37a76e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6096, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006096__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6097-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6097, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "528937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6098, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6419", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6098", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad63e4c6-b75d-48ad-9b66-055f3c1e8f8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6097, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006097__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6098-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6098, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6099, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6420", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6099", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2a485ef-54bf-4102-a1dd-5edb374b0ac6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6098, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006098__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6099-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6099, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6100, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6421", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6100", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53d01e7b-ae18-4001-88c1-fb28582b3ce7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6099, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006099__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6100-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6100, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6101, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6422", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6101", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0d158f0-97de-4222-b33b-c1b9679c95c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6100, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006100__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6101-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6101, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6102, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6423", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6102", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1c156e7-f681-4f12-9d42-02805a2f42fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6101, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006101__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6102-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6102, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6103, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6424", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6103", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "885560e8-7656-4835-a285-8f77f9af18ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6102, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006102__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6103-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6103, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6104, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6425", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6104", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "831885e2-3182-4d66-bcc5-83c9afdcbad8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6103, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006103__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6104-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6104, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6105, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6426", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6105", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b1e1002-3943-43e3-8d64-7c3895ffc9a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6104, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006104__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6105-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6105, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6106, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6427", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6106", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01aa9b29-e0f9-493d-87d8-590f85aa0a7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6105, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006105__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6106-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6106, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6107, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6428", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6107", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaac55c3-cb41-46b4-a3c6-e1907de0325b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 529924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6106, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006106__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6107-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6107, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6108, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6429", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6108", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d9dbe8a-481b-40fd-925a-4902b7f6d0d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6107, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006107__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6108-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6108, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6109, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6430", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6109", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0117e2d-ca4b-4376-8642-69e11d72ae37", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6108, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006108__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6109-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6109, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "529968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6110, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6431", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6110", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8456f1d-9536-4307-b819-10c3aef33c59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6109, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006109__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6110-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6110, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6111, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6432", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6111", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "450a6484-caa4-44a2-929c-21782d0cfa6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6110, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006110__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6111-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6111, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6112, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6433", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6112", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "689fdfd1-3516-41f8-90e4-f49219a0e68d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6111, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006111__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6112-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6112, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6113, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6434", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6113", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd88ce60-d9f4-41e9-a88e-41b253f6e0bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6112, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006112__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6113-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6113, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6114, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6435", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6114", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1c60e4c-35e8-4a4a-b6e8-827abe52dad8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6113, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006113__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6114-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6114, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6115, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6436", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6115", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "212b8b24-15d1-4816-8696-74b76ad8b016", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6114, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006114__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6115-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6115, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6116, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6437", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6116", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "345b0b92-cc38-4740-95c1-3bd3035c60f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6115, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006115__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6116-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6116, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6117, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6438", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6117", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "925f3590-c3be-4c2e-a293-686e0c60ad3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6116, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006116__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6117-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6117, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6118, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6439", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6118", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b771ea0-0db4-4f00-adc7-66fdfe6eb98d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6117, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006117__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6118-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6118, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6119, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6440", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6119", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27befb1e-00f2-4957-ad48-aa8891025b63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 530971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6118, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006118__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6119-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6119, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6120, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6441", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6120", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38fbbade-a2d6-483a-bb0d-90d2940fd367", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6119, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006119__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6120-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6120, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "530921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6121, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6442", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6121", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7bfa42e9-55bb-4c19-81c6-4c59b9d426d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6120, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006120__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6121-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6121, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6122, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6443", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6122", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d3b5029-a334-4476-bd5f-d2c1aac2c25a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6121, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006121__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6122-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6122, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6123, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6444", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6123", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c107ef3-7687-4151-ab75-8906bf8493a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6122, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006122__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6123-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6123, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6124, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6445", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6124", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8becc77-6f19-4f37-8cf6-dfcb752e7a5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6123, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006123__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6124-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6124, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6125, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6446", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6125", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c27723bb-ef5f-498a-a293-2d687e6455b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6124, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006124__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6125-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6125, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6126, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6447", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6126", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40c69c5f-9ca6-45e2-b07b-33ae6438afff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6125, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006125__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6126-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6126, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6127, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6448", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6127", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbd7ab72-a8b4-4bc3-9d6f-48ccca1901e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6126, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006126__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6127-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6127, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6128, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6449", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6128", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d6d6d5e-0981-4642-aafe-8dbf13ae667c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6127, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006127__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6128-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6128, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6129, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6450", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6129", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "663d8a7d-9a13-453f-b6b0-791f1499b19d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6128, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006128__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6129-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6129, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6130, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6451", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6130", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc251638-57d6-40a1-b1dc-433b1ed13362", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 531924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6129, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006129__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6130-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6130, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6131, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6452", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6131", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b32d8ecc-c994-4621-8b8e-a239c1ee7403", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6130, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006130__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6131-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6131, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6132, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6453", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6132", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3facde8f-6e0a-42b7-90c8-5717f395b350", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6131, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006131__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6132-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6132, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "531968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6133, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6454", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6133", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4766c47-95e4-4013-a17c-6622437fb275", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6132, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006132__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6133-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6133, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6134, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6455", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6134", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86e3d772-6c7d-4d7f-af97-5f93b01a6d26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6133, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006133__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6134-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6134, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6135, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6456", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6135", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da224665-b0d5-415d-8d5a-06620489d620", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6134, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006134__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6135-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6135, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6136, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6457", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6136", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21f9a44e-5e6c-4084-a7d9-eacf6664f545", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6135, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006135__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6136-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6136, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6137, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6458", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6137", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5125da0-8f4f-40e9-bf0a-8658dd78cd00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6136, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006136__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6137-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6137, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6138, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6459", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6138", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d521abbc-f0b9-400a-bd1e-dd92a3524562", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6137, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006137__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6138-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6138, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6139, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6460", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6139", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71c82daa-f81e-4337-a2d0-0cac1452fbaf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6138, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006138__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6139-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6139, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6140, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6461", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6140", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3753c7f9-bbe6-48ab-867e-3e1695dff87f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6139, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006139__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6140-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6140, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6141, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6462", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6141", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd68a791-c6ab-4309-8e0c-a71186dc86f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6140, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006140__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6141-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6141, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6142, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6463", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6142", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08662322-a6e2-4950-bcee-715292faa03a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 532971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6141, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006141__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6142-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6142, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6143, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6464", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6143", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac7c7c08-a088-4c8b-adf1-3dd519976fe4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6142, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006142__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6143-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6143, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "532921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6144, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6465", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6144", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e62f50c-2242-4a02-a57a-00756a13337e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6143, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006143__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6144-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6144, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6145, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6466", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6145", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4e7a72d-650d-4fad-8aef-016802f09498", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6144, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006144__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6145-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6145, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6146, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6467", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6146", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a38cf9e-8046-4d70-a471-0bdecdc2c5d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6145, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006145__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6146-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6146, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6147, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6468", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6147", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4854786a-7a3e-4a4c-a939-f92012283d9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6146, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006146__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6147-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6147, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6148, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6469", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6148", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32b21f38-40e6-47e3-b0c2-cc23c975e66d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6147, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006147__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6148-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6148, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6149, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6470", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6149", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "604b795d-0f1b-4783-867c-fb1a2a03a698", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6148, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006148__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6149-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6149, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6150, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6471", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6150", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47d602eb-2821-4ba6-b190-7dd8eca6eb1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6149, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006149__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6150-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6150, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6151, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6472", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6151", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cd0cf7e-25ae-43cc-a76c-8da283946b49", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6150, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006150__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6151-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6151, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6152, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6473", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6152", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "03370d8f-d290-4fdf-87a6-54f25f0901f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6151, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006151__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6152-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6152, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6153, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6474", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6153", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e752451-9b52-4d4e-8870-330645699e9c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 533924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6152, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006152__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6153-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6153, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6154, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6475", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6154", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d3377b5-d601-4ebe-b3da-506e051db294", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6153, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006153__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6154-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6154, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6155, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6476", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6155", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "067166af-112b-450b-93bd-96e76253879c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6154, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006154__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6155-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6155, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "533953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6156, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6477", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6156", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30a18abf-59b8-4a89-8fb9-41c0122b6fc7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6155, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006155__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6156-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6156, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6157, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6478", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6157", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa1b70c4-ab4e-491b-a7de-dd562d09f6b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6156, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006156__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6157-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6157, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6158, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6479", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6158", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8753d8a-0c22-4c91-8a77-a4f88ae4bc62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6157, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006157__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6158-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6158, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6159, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6480", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6159", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd9deb6d-6315-4458-b4ec-84040096b7f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6158, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006158__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6159-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6159, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6160, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6481", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6160", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "438579cb-5253-462d-aeaf-530077de4df8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6159, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006159__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6160-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6160, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6161, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6482", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6161", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ff73c44-53e3-4512-a945-c924cedb0aa6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6160, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006160__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6161-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6161, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6162, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6483", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6162", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e98f9f4-67e2-43df-bcc0-fcab5aca5d70", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6161, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006161__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6162-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6162, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6163, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6484", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6163", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebab716f-0b75-45d9-b296-ace18b15963b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6162, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006162__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6163-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6163, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6164, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6485", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6164", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1f1613a-1cd4-4cff-b0fa-fb44845fc2c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6163, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006163__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6164-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6164, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6165, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6486", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6165", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52fd036d-1ecb-4012-b422-25e334ad1b31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 534955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6164, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006164__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6165-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6165, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6166, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6487", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6166", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "999c2059-c921-4a51-9c8c-200ec5cba72c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6165, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006165__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6166-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6166, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "534921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6167, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6488", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6167", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5b550cdb-755a-4764-9371-bcbc9f27faa1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6166, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006166__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6167-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6167, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6168, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6489", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6168", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb0e5574-4207-4e11-9f42-b34b21a8d233", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6167, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006167__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6168-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6168, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6169, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6490", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6169", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c67edb6b-fc29-44aa-b03f-86731331b1cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6168, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006168__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6169-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6169, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6170, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6491", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6170", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7077c29b-76d2-4058-aca9-ddc53e670f8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6169, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006169__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6170-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6170, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6171, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6492", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6171", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2733f282-36d6-407b-ac92-2a4f3b9cf9c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6170, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006170__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6171-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6171, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6172, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6493", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6172", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8cb9a52-0557-4e39-a4ad-4fe66d481d75", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6171, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006171__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6172-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6172, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6173, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6494", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6173", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "777f2f81-1b0c-4811-88e7-3ff9f0089d19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6172, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006172__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6173-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6173, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6174, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6495", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6174", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e655a641-3ec7-4753-b277-5873fa30a30a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6173, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006173__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6174-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6174, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6175, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6496", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6175", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a0b159e-abef-4d9b-a252-6c79a5b4e678", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6174, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006174__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6175-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6175, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6176, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6497", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6176", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5262ff89-2d46-4642-a881-c305f79d9422", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 535924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6175, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006175__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6176-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6176, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6177, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6498", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6177", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45d64087-7071-4e83-8153-6526fac6bd90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6176, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006176__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6177-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6177, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6178, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6499", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6178", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e38aa4a-5dd2-4704-a01e-aac792242066", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6177, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006177__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6178-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6178, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "535953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6179, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6500", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6179", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af14f752-ce9c-46ce-be38-fe8524a14963", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6178, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006178__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6179-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6179, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6180, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6501", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6180", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0590dc9-e410-4ea5-9be9-f2d8a38b101e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6179, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006179__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6180-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6180, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6181, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6502", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6181", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9abcfb86-c886-45d4-81ed-cb86e9c5f91d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6180, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006180__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6181-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6181, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6182, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6503", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6182", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee41f9c0-b81f-4626-a055-b84cfd44fe96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6181, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006181__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6182-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6182, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6183, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6504", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6183", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf87bd5c-1ba0-4a6a-827b-315572a513ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6182, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006182__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6183-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6183, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6184, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6505", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6184", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d90297c4-1c5d-4b6c-bc77-545309d0afce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6183, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006183__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6184-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6184, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6185, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6506", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6185", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16cc6eb2-9127-4e73-b168-0bfd4bdbadc2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6184, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006184__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6185-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6185, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6186, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6507", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6186", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00d3bcd6-ea24-481c-b2c8-842ef48e5c7e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6185, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006185__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6186-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6186, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6187, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6508", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6187", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ec1e410-57fb-4b8b-814c-378b117312f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6186, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006186__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6187-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6187, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6188, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6509", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6188", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d08f56d-e607-44cc-92ff-f8882f8e1375", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 536955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6187, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006187__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6188-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6188, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6189, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6510", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6189", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ce753b2-2410-4b4c-9fe3-0d73fbd95adf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6188, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006188__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6189-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6189, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "536906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6190, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6511", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6190", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d354b7a-73a5-4bb0-b05d-d2842e2b967c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6189, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006189__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6190-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6190, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6191, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6512", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6191", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36053e0d-acbb-42b5-8671-c6b3871de16f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6190, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006190__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6191-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6191, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6192, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6513", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6192", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c92ed2d-79e0-4e7c-9b21-ba839c20de79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6191, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006191__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6192-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6192, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6193, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6514", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6193", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2107b4d2-c3d7-46e4-969b-81fff6751e20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6192, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006192__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6193-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6193, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6194, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6515", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6194", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5be77707-9fca-46b8-992d-d5a9e8105166", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6193, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006193__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6194-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6194, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6195, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6516", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6195", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a57a3a51-3a34-41e5-b4cc-5fe3a80b83a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6194, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006194__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6195-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6195, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6196, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6517", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6196", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b11fc97-b666-4f56-87aa-85907c298342", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6195, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006195__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6196-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6196, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6197, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6518", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6197", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9362d25b-5b76-4543-8fe5-76d3947ef905", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6196, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006196__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6197-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6197, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6198, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6519", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6198", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e8abae1-3bec-4f33-ae9f-f2fe66607968", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6197, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006197__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6198-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6198, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6199, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6520", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6199", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc400e17-e349-4f28-a7bc-5c472c6f7c8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 537908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6198, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006198__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6199-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6199, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6200, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6521", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6200", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8297678a-d2e8-4475-ad20-a046a6dce24a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6199, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006199__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6200-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6200, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6201, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6522", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6201", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5f10c9bb-af11-42ec-aba2-25b997d59258", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6200, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006200__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6201-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6201, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "537953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6202, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6523", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6202", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0237d27-86d4-4c6d-8da1-5e9ebaf005e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6201, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006201__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6202-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6202, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6203, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6524", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6203", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa4c0c2d-fb6a-4928-b26d-8fdc8fb4a935", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6202, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006202__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6203-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6203, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6204, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6525", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6204", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "983e9266-1827-467d-a7fc-d66583bae8d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6203, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006203__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6204-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6204, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6205, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6526", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6205", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8748770-1f0c-4d23-aa76-967b357b79fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6204, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006204__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6205-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6205, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6206, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6527", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6206", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13debc1d-00e1-4025-ba9e-3d3f5c986f2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6205, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006205__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6206-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6206, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6207, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6528", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6207", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21c39c0a-fd16-498a-9338-2a6fb2ca4e33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6206, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006206__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6207-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6207, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6208, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6529", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6208", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50e14af1-bcf1-4bfd-9e8c-e96436b6b818", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6207, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006207__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6208-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6208, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6209, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6530", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6209", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b956ce44-ec5e-4cc1-9d2b-bac571ead4e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6208, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006208__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6209-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6209, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6210, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6531", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6210", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf5050e7-6925-49d3-ba88-65233f33175b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6209, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006209__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6210-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6210, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6211, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6532", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6211", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5ced0ae-2248-4486-9a23-ea3c05a6740e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 538955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6210, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006210__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6211-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6211, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6212, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6533", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6212", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8b96ffb-ed31-4f33-b314-c2478a84bb85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6211, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006211__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6212-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6212, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6213, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6534", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6213", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "332f66aa-26eb-4bac-a9d1-e40fc18bb643", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6212, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006212__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6213-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6213, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "538984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6214, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6535", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6214", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8d32f28-c746-4cb0-9f82-3e2272a656ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6213, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006213__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6214-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6214, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6215, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6536", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6215", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1bfeb80-a83d-4351-8bbc-295e905e745b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6214, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006214__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6215-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6215, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6216, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6537", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6216", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dce5ea08-aa07-4c82-9c2e-2507d00249d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6215, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006215__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6216-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6216, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6217, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6538", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6217", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "373677f9-9147-48aa-a623-663b6bde4d12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6216, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006216__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6217-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6217, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6218, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6539", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6218", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30236b82-7a33-46d4-8317-019452e14d39", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6217, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006217__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6218-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6218, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6219, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6540", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6219", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c821de99-2b06-4fb5-8fb2-d7f56e0ab594", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6218, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006218__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6219-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6219, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6220, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6541", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6220", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "976f6187-8818-465a-806a-18669d29eb9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6219, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006219__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6220-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6220, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6221, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6542", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6221", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f4202b5-668d-4ee7-b8dc-7dc38c6d7d44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6220, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006220__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6221-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6221, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6222, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6543", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6222", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efd983ae-c708-42f1-ac4c-29e7ce1a2f1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6221, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006221__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6222-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6222, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6223, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6544", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6223", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "839ef118-5be2-4352-9325-0250a046060c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 539986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6222, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006222__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6223-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6223, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6224, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6545", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6224", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ec89d61-47c7-4d75-83ac-3c2659d99f8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6223, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006223__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6224-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6224, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "539953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6225, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6546", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6225", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18d751d0-541c-4dbb-89bf-0af7c2461076", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6224, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006224__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6225-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6225, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6226, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6547", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6226", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce3f27e4-6506-496d-a90f-63bc26c66f03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6225, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006225__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6226-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6226, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6227, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6548", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6227", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbf8b0b5-0d5a-4e12-bc5f-e0b3fc4129d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6226, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006226__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6227-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6227, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6228, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6549", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6228", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db0ef744-380a-444a-9f6d-64eddf0876fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6227, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006227__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6228-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6228, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6229, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6550", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6229", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21e17ed8-9092-4507-a896-c98656e57158", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6228, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006228__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6229-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6229, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6230, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6551", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6230", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf028dd9-de07-4d7e-8ff1-2a48b2f0ce1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6229, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006229__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6230-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6230, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6231, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6552", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6231", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0072b683-d376-4dd3-a7fc-f53d088d54d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6230, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006230__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6231-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6231, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6232, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6553", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6232", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd391e7a-85ad-4e28-b9ad-30696691865b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6231, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006231__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6232-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6232, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6233, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6554", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6233", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6424f494-e739-4825-a8bd-c7adf8e3a4a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6232, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006232__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6233-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6233, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6234, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6555", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6234", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f011be87-ef5e-446e-9966-b4b8a1e8f587", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 540940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6233, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006233__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6234-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6234, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6235, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6556", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6235", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc132d04-3047-4025-893d-8ce788a7564a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6234, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006234__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6235-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6235, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6236, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6557", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6236", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0d1eb1d-909a-49da-8ebd-4b559d4cec35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6235, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006235__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6236-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6236, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "540984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6237, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6558", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6237", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "951b7009-030b-402f-8ff3-1dbcee4dda4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6236, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006236__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6237-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6237, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6238, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6559", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6238", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d41cbdc-e6e0-41c8-9c6f-420944c93037", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6237, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006237__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6238-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6238, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6239, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6560", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6239", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae53e7f4-2412-4a44-9190-628dfa386b6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6238, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006238__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6239-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6239, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6240, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6561", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6240", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "173be2c3-64e0-4d98-bf2d-a3fbeb9ad7d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6239, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006239__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6240-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6240, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6241, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6562", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6241", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fcda8bd-9d2b-456b-81de-69f2272e56fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6240, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006240__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6241-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6241, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6242, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6563", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6242", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac4eaa72-81a0-4d42-900e-c14a18031076", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6241, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006241__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6242-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6242, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6243, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6564", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6243", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97dc8d94-3b89-4070-a78d-252ede7121d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6242, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006242__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6243-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6243, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6244, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6565", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6244", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17936638-5bde-468a-9dcf-4222b541387e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6243, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006243__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6244-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6244, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6245, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6566", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6245", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c69bfe3-8da7-4496-ac0a-e64450634eb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6244, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006244__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6245-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6245, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6246, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6567", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6246", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f642dafd-a86b-4169-914e-763a97490ba7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 541986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6245, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006245__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6246-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6246, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6247, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6568", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6247", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7111c946-582a-4e0e-b19a-59f97423f34b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6246, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006246__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6247-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6247, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "541937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6248, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6569", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6248", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8d850a8-b446-4d25-af72-d8b2cee42bbd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6247, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006247__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6248-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6248, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6249, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6570", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6249", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21d29387-6116-44bc-aef9-6b099279366c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6248, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006248__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6249-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6249, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6250, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6571", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6250", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54b1195e-96a2-4a00-98b9-e280e1888eb3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6249, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006249__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6250-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6250, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6251, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6572", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6251", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51745059-6cee-4b60-92ba-bb9263cb67af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6250, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006250__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6251-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6251, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6252, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6573", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6252", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f35f9b76-b398-4ae5-8134-e8a3cc638be3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6251, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006251__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6252-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6252, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6253, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6574", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6253", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccfa4fb5-bbaa-4c84-b045-56bc054c0c1c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6252, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006252__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6253-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6253, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6254, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6575", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6254", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc18484d-c30c-408d-ace0-a7e2c519da91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6253, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006253__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6254-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6254, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6255, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6576", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6255", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cd66252-5ba5-41ea-9a37-ca3d0d8b874f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6254, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006254__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6255-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6255, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6256, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6577", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6256", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41e7b153-f889-45bd-a9d4-37f7056b234a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6255, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006255__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6256-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6256, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6257, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6578", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6257", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f716f5e0-be55-486b-b012-52ff74b9ab03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 542940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6256, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006256__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6257-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6257, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6258, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6579", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6258", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a1b9352-620e-4414-9bdf-e66ff1da1f9c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6257, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006257__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6258-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6258, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6259, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6580", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6259", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e47bb50-d34e-4759-b1bb-8f3d5f977a23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6258, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006258__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6259-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6259, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "542984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6260, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6581", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6260", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f0e59c9-5f01-466a-b3e6-9f9bfa80b39c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6259, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006259__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6260-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6260, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6261, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6582", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6261", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9eb527da-635c-4881-b637-37c9fc6a60f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6260, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006260__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6261-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6261, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6262, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6583", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6262", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dfccbf93-5015-41b5-a791-ac080c54c232", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6261, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006261__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6262-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6262, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6263, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6584", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6263", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27e65931-771d-453e-b1ab-2e9af2bb5ebc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6262, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006262__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6263-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6263, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6264, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6585", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6264", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58a01327-f082-4c1a-9224-7ebb702ea9d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6263, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006263__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6264-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6264, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6265, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6586", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6265", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28fbf799-ea91-4479-8d41-1c16e3b6cf69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6264, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006264__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6265-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6265, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6266, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6587", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6266", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f284c5b2-0921-4763-b138-a8cc1205608f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6265, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006265__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6266-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6266, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6267, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6588", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6267", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc5bdd5e-db22-43b6-a080-1d73d9b8513d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6266, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006266__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6267-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6267, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6268, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6589", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6268", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b06a6fef-d9c7-4e04-ac6f-4c2574b75e35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6267, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006267__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6268-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6268, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6269, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6590", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6269", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4ee441e-4abc-419a-8da4-1fb7bed1c455", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 543986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6268, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006268__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6269-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6269, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6270, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6591", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6270", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96935ac6-3fda-4d06-964b-abc7e88cb50c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6269, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006269__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6270-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6270, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "543937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6271, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6592", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6271", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "527e9952-9502-4204-988b-bb92782b6ec2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6270, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006270__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6271-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6271, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6272, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6593", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6272", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5be6e91-41b6-45c7-8fd8-cb82274c47d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6271, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006271__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6272-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6272, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6273, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6594", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6273", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8c1a667-7f99-4a31-a680-d5a01b8a9339", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6272, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006272__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6273-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6273, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6274, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6595", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6274", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4af0801f-1b57-44c2-a214-11ed527c2ded", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6273, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006273__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6274-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6274, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6275, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6596", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6275", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74c38a02-c6cb-4e28-9e46-7f5c68ede5ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6274, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006274__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6275-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6275, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6276, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6597", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6276", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19ecca8b-7a67-47cc-8527-2a2010852826", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6275, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006275__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6276-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6276, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6277, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6598", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6277", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a6768ef-9bd6-4460-ab11-76454825632b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6276, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006276__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6277-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6277, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6278, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6599", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6278", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9534c248-10cd-4a6b-a058-aa6ff1282ac2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6277, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006277__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6278-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6278, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6279, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6600", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6279", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37df1641-e5cc-49e0-8757-1f26fe79a062", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6278, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006278__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6279-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6279, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6280, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6601", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6280", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c9a621a-305d-4e81-b153-1ad10fb24538", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 544940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6279, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006279__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6280-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6280, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6281, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6602", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6281", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baffb0b3-ac9f-49fa-9471-ea888cb57852", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6280, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006280__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6281-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6281, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6282, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6603", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6282", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c68bec37-a898-46ee-b343-54e33b3dbe99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6281, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006281__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6282-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6282, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "544968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6283, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6604", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6283", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5502603e-d5a7-4430-9ab7-50f3c5db72a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6282, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006282__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6283-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6283, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6284, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6605", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6284", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32469cb5-cc76-44e6-9812-31f671393956", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6283, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006283__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6284-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6284, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6285, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6606", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6285", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fac4d66-3a13-4d7f-a13e-ad931a9e61ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6284, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006284__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6285-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6285, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6286, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6607", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6286", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6e72c9f-2b7d-4711-8f0b-cb1446bf43a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6285, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006285__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6286-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6286, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6287, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6608", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6287", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d13e85cd-f69d-432e-a35d-945485d7e650", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6286, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006286__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6287-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6287, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6288, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6609", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6288", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4137878-3b5b-4ea0-a5ae-c52e004d6be5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6287, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006287__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6288-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6288, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6289, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6610", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6289", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7579aba7-e71b-4cc7-8666-5c3a65406b95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6288, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006288__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6289-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6289, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6290, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6611", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6290", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "348a1e06-3860-4aa6-a670-1102e532ccad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6289, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006289__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6290-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6290, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6291, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6612", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6291", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f364268-3086-464b-9535-74da6e94b21e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6290, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006290__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6291-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6291, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6292, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6613", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6292", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7731a98-fc9f-4d2a-a43c-069c42e72a28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 545971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6291, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006291__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6292-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6292, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6293, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6614", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6293", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38c39f4c-968b-4f55-806e-a5b60492bc09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6292, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006292__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6293-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6293, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "545937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6294, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6615", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6294", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ad5037c0-8a7b-4f6f-8330-f164d2457c5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6293, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006293__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6294-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6294, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6295, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6616", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6295", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "514dcd4e-0f80-459a-9ba9-dcbabdffc0e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6294, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006294__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6295-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6295, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6296, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6617", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6296", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9bc1d4b-dd7c-4ed4-81ed-b98129ce3567", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6295, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006295__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6296-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6296, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6297, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6618", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6297", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aba5f38e-99f0-4eee-abf9-50918e68adef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6296, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006296__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6297-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6297, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6298, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6619", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6298", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39d82c2f-98f4-4e19-8af8-68cabecd86aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6297, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006297__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6298-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6298, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6299, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6620", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6299", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "756e5afd-c1cb-4ba2-991a-078e7062d87f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6298, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006298__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6299-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6299, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6300, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6621", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6300", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dfc65831-8d7a-4bc4-9740-5706ad36a6d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6299, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006299__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6300-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6300, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6301, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6622", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6301", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dfd9f48c-290b-4cd2-b2d4-e65f3dfc0a4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6300, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006300__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6301-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6301, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6302, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6623", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6302", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e35d6c78-4532-4be7-9db1-a2c5f0dbc6d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6301, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006301__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6302-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6302, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6303, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6624", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6303", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11f37a70-ebc4-464b-abc3-832a0a531c59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 546940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6302, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006302__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6303-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6303, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6304, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6625", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6304", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87e8427e-46d2-45f1-b2d9-1af178c7a311", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6303, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006303__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6304-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6304, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6305, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6626", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6305", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8824c9b4-5c36-4f8f-ade9-67c567df84b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6304, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006304__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6305-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6305, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "546968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6306, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6627", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6306", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "000ccdf4-b9c0-446a-9a53-d209a9415a8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6305, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006305__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6306-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6306, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6307, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6628", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6307", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa10cf83-4e04-4fd3-9fb8-6478ad79b2ea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6306, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006306__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6307-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6307, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6308, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6629", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6308", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3247f3f9-f5d2-4fd0-972e-156f31ce5e8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6307, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006307__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6308-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6308, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6309, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6630", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6309", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28c5a003-5101-4ef0-a2bf-253773acd90f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6308, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006308__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6309-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6309, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6310, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6631", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6310", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8fbd0c69-a001-455a-b1c5-c36d318bf92a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6309, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006309__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6310-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6310, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6311, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6632", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6311", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15071d56-02a1-498f-8b58-aae1262b8304", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6310, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006310__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6311-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6311, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6312, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6633", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6312", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d53f37a2-1cea-4585-b502-9a47e7a09d9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6311, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006311__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6312-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6312, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6313, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6634", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6313", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c324e636-a0ad-4c9a-a0ee-00008fe167c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6312, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006312__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6313-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6313, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6314, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6635", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6314", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2a268f4d-47a2-4317-a8fd-ca4cf3656790", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6313, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006313__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6314-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6314, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6315, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6636", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6315", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c20e79c0-af57-49d1-a965-bef23a7aaa2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 547971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6314, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006314__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6315-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6315, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6316, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6637", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6316", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02357568-af29-41d6-bb22-bda9b845faa8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6315, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006315__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6316-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6316, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "547921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6317, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6638", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6317", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "91a42afc-86df-4397-ac9d-b8d8be86bc7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6316, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006316__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6317-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6317, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6318, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6639", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6318", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b59b4e12-2d70-4079-aa1e-3102fb4d17af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6317, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006317__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6318-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6318, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6319, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6640", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6319", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88b4cdcd-e410-4ed1-8696-f822795b0ec6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6318, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006318__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6319-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6319, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6320, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6641", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6320", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4eb8ec2d-7151-4bd7-a0d7-58f16d52f0bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6319, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006319__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6320-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6320, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6321, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6642", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6321", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04f7c0d3-4898-466c-8450-131e1afe195e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6320, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006320__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6321-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6321, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6322, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6643", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6322", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9843a060-96f0-4130-82c2-dbd83205aff6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6321, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006321__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6322-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6322, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6323, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6644", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6323", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6dbc7a8b-7901-468e-a15e-3caba2b0c23f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6322, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006322__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6323-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6323, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6324, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6645", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6324", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25c093ec-03b1-411b-9549-b7efc0e313ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6323, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006323__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6324-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6324, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6325, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6646", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6325", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e5e0d06-169e-40cf-b961-63c0adcbeba7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6324, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006324__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6325-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6325, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6326, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6647", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6326", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "181e0c15-9313-4e32-94aa-521bfa7bd88a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6325, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006325__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6326-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6326, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6327, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6648", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6327", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3c0e3e1-fa48-4ab0-87bf-ea3246622232", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6326, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006326__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6327-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6327, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6328, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6649", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6328", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "202fd8aa-4ea5-42b9-a6dc-9438badd67cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6327, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006327__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6328-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6328, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "548968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6329, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6650", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6329", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5e8486f-05f8-43a4-be64-736173bd9d03", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6328, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006328__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6329-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6329, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6330, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6651", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6330", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7a25e02-a7a7-461a-921c-9e90bcad098b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6329, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006329__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6330-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6330, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6331, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6652", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6331", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "214d35b6-d2bd-4bad-8cf1-652aa7f75c45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6330, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006330__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6331-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6331, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6332, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6653", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6332", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b187edc8-2df0-453a-8c98-45836ec01708", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6331, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006331__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6332-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6332, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6333, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6654", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6333", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "078ab8a7-38c6-4872-8494-b87bec67342e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6332, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006332__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6333-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6333, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6334, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6655", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6334", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b592f4c7-a231-4a91-b508-d634ce4ed299", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6333, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006333__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6334-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6334, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6335, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6656", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6335", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90fcb91a-230e-4231-a11b-808f3940450b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6334, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006334__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6335-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6335, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6336, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6657", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6336", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "076aaa97-5711-4852-a127-a1291c68c6bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6335, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006335__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6336-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6336, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6337, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6658", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6337", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24dbd2b3-d1c1-491a-93ed-2ac88c29b579", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6336, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006336__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6337-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6337, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6338, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6659", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6338", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8cb384c7-42e1-42a9-92a7-1df3cfa951b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 549971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6337, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006337__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6338-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6338, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6339, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6660", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6339", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c984407c-14e6-4a80-88c4-a9fee5e8854c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6338, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006338__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6339-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6339, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "549921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6340, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6661", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6340", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4fbcff25-8720-4bea-8fe3-1cba0f6f7a0f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6339, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006339__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6340-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6340, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6341, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6662", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6341", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a203dedf-396b-4dc1-b38b-d8f53b8222f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6340, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006340__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6341-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6341, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6342, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6663", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6342", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c76dd574-a84e-4b9a-9ae9-8f9d31132747", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6341, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006341__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6342-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6342, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6343, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6664", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6343", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f1cc513-a33b-4319-ada1-c2542220ef2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6342, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006342__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6343-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6343, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6344, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6665", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6344", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68da683b-ec4b-4fe5-bdee-c48a7f4fd1bf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6343, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006343__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6344-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6344, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6345, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6666", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6345", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98d8647c-33da-405e-a984-60253f9b1e16", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6344, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006344__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6345-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6345, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6346, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6667", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6346", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf29763f-d9a1-4515-ba3d-81f38e1f759c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6345, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006345__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6346-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6346, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6347, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6668", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6347", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbe5a51d-49d1-4edb-a453-181baf887418", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6346, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006346__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6347-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6347, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6348, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6669", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6348", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ac291a6-083a-46c9-aa1e-c99724dd007a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6347, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006347__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6348-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6348, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6349, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6670", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6349", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e477f289-e582-47d6-aab6-e13ea5e9b7ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 550924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6348, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006348__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6349-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6349, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6350, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6671", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6350", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f2abb1f-8e99-4dfb-84dd-d9dd0453af33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6349, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006349__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6350-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6350, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6351, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6672", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6351", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cac292dc-8528-4573-86f9-d346ae5f9021", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6350, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006350__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6351-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6351, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "550968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6352, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6673", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6352", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a68022e-4f20-4880-8e54-ba75f1ec7709", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6351, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006351__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6352-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6352, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6353, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6674", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6353", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb77a2b9-4570-46e8-98a1-10c3e11447c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6352, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006352__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6353-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6353, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6354, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6675", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6354", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8490c8eb-b48d-4cc6-8021-d7f06243c51d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6353, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006353__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6354-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6354, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6355, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6676", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6355", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f7aeeef-e539-4d61-9d8a-e831a9cf327c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6354, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006354__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6355-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6355, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6356, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6677", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6356", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a67d5b79-e152-40b4-b059-e188a29290af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6355, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006355__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6356-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6356, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6357, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6678", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6357", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6d9b174-628a-4a06-ab45-ddd2ec32806d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6356, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006356__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6357-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6357, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6358, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6679", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6358", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d14041bc-4644-468a-9149-5b3946b9604b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6357, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006357__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6358-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6358, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6359, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6680", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6359", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "801fafb1-442a-450d-997d-32469d87a80c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6358, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006358__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6359-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6359, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6360, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6681", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6360", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55f57202-041e-4d20-856e-30abdd7db3d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6359, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006359__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6360-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6360, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6361, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6682", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6361", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5ef00b2-d5b1-4eda-bd4a-b1882959e879", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 551955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6360, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006360__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6361-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6361, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6362, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6683", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6362", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c785b55e-c180-4a54-8b81-50d1bb289feb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6361, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006361__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6362-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6362, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "551921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6363, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6684", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6363", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "303d696f-1e99-4f07-aba8-09b56466b858", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6362, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006362__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6363-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6363, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6364, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6685", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6364", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04902768-55ff-4eac-b758-400d06515c69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6363, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006363__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6364-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6364, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6365, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6686", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6365", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e12b967-5003-488f-a678-1499a12c603b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6364, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006364__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6365-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6365, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6366, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6687", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6366", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f40429e8-dd6a-428e-9f05-120b6e51759b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6365, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006365__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6366-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6366, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6367, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6688", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6367", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cac844d3-5699-4db1-bdcd-52fcf3f3d238", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6366, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006366__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6367-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6367, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6368, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6689", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6368", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b63fba3-a31a-4dfd-ae84-b6af8e19b3a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6367, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006367__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6368-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6368, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6369, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6690", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6369", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c81e0793-480d-41a9-a50e-7cf3d4c245b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6368, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006368__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6369-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6369, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6370, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6691", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6370", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98c3bafe-6799-4117-b357-a2f956ae911c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6369, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006369__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6370-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6370, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6371, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6692", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6371", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8979a6e-f4a1-4457-a5cf-967604e6ab1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6370, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006370__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6371-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6371, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6372, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6693", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6372", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a1ef330-4e93-48ca-a8e1-d1e3c188928a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 552924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6371, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006371__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6372-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6372, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6373, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6694", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6373", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85ae16c7-30ba-4421-a1bc-b6afc9847fc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6372, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006372__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6373-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6373, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6374, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6695", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6374", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8918254-3f06-47da-bce5-2d396a6ed3ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6373, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006373__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6374-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6374, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "552953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6375, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6696", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6375", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89793033-bc29-447e-9126-7d68f7807f6f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6374, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006374__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6375-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6375, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6376, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6697", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6376", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79e013e0-b3ad-42e0-8fb6-f4d9d8a6b16d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6375, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006375__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6376-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6376, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6377, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6698", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6377", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "543bda6d-fff8-4ead-8ba8-f69eb56fcf01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6376, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006376__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6377-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6377, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6378, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6699", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6378", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c507f493-07af-4784-a5b0-1bfef4ca31ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6377, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006377__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6378-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6378, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6379, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6700", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6379", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f29925c8-c26c-4f4b-b997-21c50a5c431f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6378, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006378__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6379-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6379, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6380, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6701", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6380", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "206e98f0-2043-496d-9c3b-f660e97c1914", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6379, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006379__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6380-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6380, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6381, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6702", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6381", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccd762b2-ceeb-4907-9db4-fd5b56e5517c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6380, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006380__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6381-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6381, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6382, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6703", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6382", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "389db929-5bd9-4333-8c11-00196d03ac41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6381, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006381__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6382-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6382, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6383, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6704", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6383", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd872e3f-075f-4a62-9775-bda1e33c71b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6382, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006382__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6383-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6383, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6384, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6705", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6384", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a56385f-49c8-460a-8584-eaa4a2804504", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 553955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6383, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006383__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6384-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6384, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6385, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6706", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6385", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3f9a652-d212-479f-8583-9db02817d81f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6384, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006384__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6385-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6385, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "553906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6386, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6707", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6386", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "134de5ec-3a31-4a7f-b040-ce0a16d06b80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6385, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006385__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6386-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6386, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6387, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6708", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6387", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "654742ef-0d76-4311-bc71-126b26fe8672", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6386, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006386__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6387-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6387, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6388, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:27:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6709", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6388", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68d43af5-9b9d-49ca-ad27-3885f584aa95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6387, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006387__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6388-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6388, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6389, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6710", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6389", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52fd180b-66ab-40c9-a11b-ba986649a7ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6388, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006388__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6389-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6389, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6390, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6711", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6390", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2429c445-ca32-4c85-b7af-6e7b3ab1186e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6389, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006389__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6390-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6390, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6391, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6712", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6391", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b65b1b0-6e4c-4dc8-aeb4-cdf7f563d530", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6390, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006390__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6391-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6391, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6392, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6713", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6392", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60732698-01d4-484c-8fc3-9b32ad4cced0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6391, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006391__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6392-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6392, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6393, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6714", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6393", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b469940-a5ab-4b37-bcf0-45a05002632e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6392, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006392__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6393-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6393, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6394, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6715", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6394", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2ec1883-d6a9-4128-9ad9-cf24ccc936f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6393, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006393__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6394-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6394, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6395, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6716", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6395", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3e40fec-d203-4cd5-9556-73f90475ba5f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 554908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6394, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006394__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6395-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6395, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6396, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6717", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6396", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c2218fb-35a1-4974-9c2f-f75ac6f75a6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6395, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006395__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6396-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6396, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6397, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6718", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6397", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bcc5886d-14ee-44da-b819-5438fcb3c170", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6396, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006396__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6397-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6397, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "554953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6398, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6719", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6398", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3382815-72a6-4bf9-8d3b-8cede726616d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6397, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006397__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6398-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6398, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6399, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6720", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6399", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4d0f912-3a9c-4516-b31a-b4e7258673fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6398, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006398__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6399-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6399, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6400, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6721", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6400", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bd92d9d-aa2d-456a-a3c1-62263a6522c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6399, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006399__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6400-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6400, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6401, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6722", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6401", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3f54361-9dd3-4a85-9d70-4381c74783a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6400, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006400__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6401-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6401, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6402, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6723", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6402", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f0889ef-d79b-4d4c-a926-278eae24568a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6401, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006401__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6402-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6402, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6403, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6724", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6403", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8225ef58-b339-4174-b7b8-88f6f012a2cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6402, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006402__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6403-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6403, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6404, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6725", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6404", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3f81003-ae2a-4569-90e1-19bedf457a4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6403, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006403__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6404-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6404, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6405, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6726", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6405", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e6ecdaf-cb8d-4fd1-b238-fab9c7ad4b71", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6404, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006404__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6405-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6405, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6406, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6727", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6406", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f9764fa-d1d4-4f5c-b1ba-34b8ef2ad4e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6405, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006405__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6406-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6406, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6407, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6728", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6407", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8dcd58c1-ac5c-4649-8f92-edaea7badcde", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 555955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6406, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006406__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6407-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6407, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6408, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6729", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6408", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5232b930-1f97-4ead-acd6-a3ed2bf2ced5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6407, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006407__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6408-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6408, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6409, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6730", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6409", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "829a2a36-2d83-4bab-86db-0ea74946ed8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6408, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006408__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6409-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6409, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "555984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6410, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6731", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6410", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e02a284c-a297-485e-b6a0-6cca8f2d5ca7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6409, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006409__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6410-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6410, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6411, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6732", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6411", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d3ddc8e-589d-41a1-9dd1-d898a0656a3a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6410, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006410__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6411-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6411, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6412, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6733", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6412", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c9fd2e1-0693-44c3-8ab8-415648a9287c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6411, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006411__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6412-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6412, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6413, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6734", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6413", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc097921-e0d1-4179-ac96-67b927d188ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6412, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006412__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6413-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6413, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6414, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6735", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6414", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b9fcec7-23bd-43d3-9379-2b2c79e95382", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6413, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006413__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6414-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6414, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6415, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6736", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6415", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "310d5d1b-c21a-4b78-8eb2-f761b92e29d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6414, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006414__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6415-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6415, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6416, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6737", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6416", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c684d4e6-d45d-4bb8-8d36-e4eab3cf1485", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6415, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006415__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6416-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6416, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6417, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6738", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6417", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a78e8cf-af20-47cb-9902-9cd828018ad9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6416, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006416__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6417-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6417, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6418, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6739", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6418", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2aecbf0a-e7d4-4f47-8d50-8b829ecf9cf5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6417, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006417__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6418-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6418, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6419, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6740", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6419", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0518727-0651-4a26-8ec0-3697942562db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 556986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6418, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006418__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6419-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6419, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6420, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6741", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6420", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d67f79a-cbb0-48f4-81a4-7aa3ddb46c66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6419, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006419__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6420-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6420, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "556953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6421, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6742", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6421", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c0939ff7-96d9-4624-872f-6ee12f7bec7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6420, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006420__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6421-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6421, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6422, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6743", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6422", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "020d7726-a05d-49b1-a8fa-456edf46b776", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6421, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006421__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6422-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6422, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6423, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6744", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6423", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f50bb96-4a19-4c24-9da2-38647ecc43dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6422, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006422__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6423-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6423, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6424, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6745", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6424", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0505d5df-a0db-4e29-ae09-83452757e7e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6423, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006423__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6424-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6424, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6425, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6746", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6425", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74c7e4dc-d4b0-412c-9776-cddf614e6cb2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6424, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006424__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6425-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6425, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6426, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6747", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6426", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc26658e-c74c-4430-9758-cfceb70c4736", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6425, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006425__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6426-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6426, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6427, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6748", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6427", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30f2ef5a-d403-4179-8b58-d09925aa51fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6426, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006426__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6427-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6427, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6428, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6749", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6428", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "442aa710-085e-448e-8b2f-dd9986d4d426", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6427, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006427__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6428-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6428, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6429, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6750", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6429", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cc85849-14ea-4c17-ba54-cdcdc0054af4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6428, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006428__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6429-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6429, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6430, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6751", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6430", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fd1a195-cab5-4a63-a940-f16f6857e0e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 557955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6429, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006429__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6430-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6430, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6431, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6752", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6431", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "055e283b-eee4-4d80-86bf-683d89eb942a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6430, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006430__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6431-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6431, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6432, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6753", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6432", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d2fa769-db59-4aa3-ab1a-76457d52daba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6431, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006431__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6432-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6432, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "557984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6433, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6754", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6433", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "454943d9-2537-4253-9864-d22fbbffc0c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6432, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006432__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6433-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6433, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6434, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6755", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6434", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a27b673-17d9-45c4-909e-f37e5932df9f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6433, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006433__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6434-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6434, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6435, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6756", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6435", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3188d487-31b1-4f17-a0be-01ca772ef225", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6434, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006434__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6435-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6435, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6436, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6757", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6436", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "519b1376-1582-40e3-b3c7-b58e2e1b9bea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6435, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006435__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6436-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6436, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6437, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6758", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6437", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "259576dd-28f4-44ef-88cd-173f47d01752", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6436, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006436__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6437-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6437, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6438, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6759", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6438", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c89b80d-afde-4f88-823f-bfa0af24c7b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6437, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006437__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6438-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6438, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6439, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6760", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6439", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa9db606-5fdc-49f1-8726-a1f8fe1363e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6438, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006438__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6439-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6439, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6440, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6761", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6440", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f356418-f88f-4d1f-9cf6-dbc99273983c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6439, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006439__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6440-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6440, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6441, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6762", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6441", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a776172e-36c5-417c-b4c0-eb9291335b3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6440, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006440__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6441-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6441, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6442, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6763", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6442", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1589d73d-2fb5-465c-a715-eeb0399c8d59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 558986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6441, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006441__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6442-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6442, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6443, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6764", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6443", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c406662c-b56b-4aca-8adc-5f7adfe6095d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6442, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006442__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6443-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6443, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "558937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6444, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6765", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6444", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5be6629c-d98d-45d3-90d8-f956ca3ff986", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6443, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006443__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6444-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6444, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6445, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6766", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6445", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5b7c45b-9007-4235-9511-1da0bdb75f3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6444, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006444__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6445-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6445, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6446, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6767", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6446", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "124c3f45-1fa4-4635-a5f1-0e6fb2eb7480", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6445, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006445__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6446-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6446, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6447, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6768", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6447", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfc8386c-2a03-41d0-8af1-967462c59558", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6446, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006446__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6447-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6447, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6448, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6769", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6448", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a00ac9c-7245-470d-a14f-71966831d3d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6447, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006447__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6448-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6448, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6449, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6770", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6449", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79b5ad21-327d-43b3-b288-b38a4b329a11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6448, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006448__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6449-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6449, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6450, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6771", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6450", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab5eca03-a844-4079-9501-46f560684667", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6449, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006449__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6450-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6450, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6451, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6772", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6451", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d41bee7f-5dd7-4f6b-9ad5-1ed7d2ec16e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6450, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006450__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6451-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6451, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6452, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6773", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6452", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8f65a2d-30e8-4c80-8926-56624ad1774f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6451, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006451__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6452-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6452, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6453, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6774", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6453", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72120c61-5546-48a1-b4b0-2f1e18044a68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 559940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6452, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006452__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6453-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6453, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6454, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6775", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6454", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd8bab24-946d-4f4e-b89e-400a3cc84b32", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6453, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006453__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6454-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6454, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6455, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6776", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6455", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a105a78-e153-4cfa-bd36-020981c7814c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6454, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006454__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6455-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6455, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "559984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6456, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6777", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6456", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21c5b469-998b-4d94-9a70-4d3ad2622bfa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6455, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006455__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6456-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6456, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6457, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6778", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6457", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdbe4071-481c-4c63-ac5b-09a8761d0b91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6456, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006456__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6457-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6457, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6458, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6779", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6458", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0f7a1c2-65a0-4dbd-bc42-1f16074f583e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6457, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006457__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6458-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6458, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6459, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6780", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6459", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "524e61f4-5449-437a-a80b-36089e7eccc2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6458, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006458__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6459-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6459, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6460, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6781", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6460", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1a1112e-ec49-4c14-8414-3be760a1a642", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6459, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006459__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6460-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6460, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6461, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6782", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6461", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18ceefde-9205-4c42-a8ef-f90eaca7e1fa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6460, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006460__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6461-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6461, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6462, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6783", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6462", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4ed4096-bd0e-45a6-8d6e-f3cb0f956667", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6461, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006461__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6462-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6462, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6463, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6784", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6463", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14e96cdc-61a7-44de-bda3-a9902449cc9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6462, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006462__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6463-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6463, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6464, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6785", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6464", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4d793d3-b51a-4685-8c3b-3201c6d6dce8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6463, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006463__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6464-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6464, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6465, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6786", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6465", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22b651ca-4614-40f6-a56c-11e004e05bc8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 560986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6464, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006464__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6465-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6465, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6466, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6787", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6466", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e971af30-9073-4a90-a168-ec5fb7b29f3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6465, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006465__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6466-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6466, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "560937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6467, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6788", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6467", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1cc41c2-fcce-4620-b2e8-842e82347d30", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6466, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006466__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6467-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6467, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6468, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6789", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6468", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3878edbd-c8b8-4d88-b213-9e65e5d42249", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6467, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006467__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6468-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6468, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6469, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6790", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6469", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc99a61e-f4ad-466f-9c3c-11c7107dc637", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6468, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006468__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6469-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6469, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6470, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6791", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6470", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e43f2989-c178-4b3e-8ac3-b19505fae23e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6469, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006469__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6470-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6470, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6471, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6792", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6471", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef749959-2499-49b2-b2e1-d9a5fcd49077", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6470, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006470__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6471-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6471, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6472, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6793", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6472", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15c15665-0adc-4236-82c8-2f3e1df0cb34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6471, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006471__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6472-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6472, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6473, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6794", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6473", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ebed720-8dbb-42c7-a856-80178bd51633", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6472, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006472__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6473-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6473, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6474, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6795", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6474", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e77f13df-0999-4bb9-8e67-0fab26a4e0cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6473, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006473__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6474-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6474, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6475, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6796", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6475", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab3041eb-49ba-4740-9afe-302d4dc82410", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6474, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006474__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6475-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6475, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6476, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6797", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6476", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e7a044e-c988-43b0-8435-a0af34f7145f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 561940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6475, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006475__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6476-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6476, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6477, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6798", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6477", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d5b27ca-8f1f-4918-880f-aae3c6d3bb3c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6476, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006476__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6477-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6477, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6478, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6799", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6478", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d78b8739-2579-47be-8b00-706d2b058788", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6477, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006477__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6478-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6478, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "561984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6479, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6800", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6479", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48690db2-f7cb-4c60-a464-fd346a64d36d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6478, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006478__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6479-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6479, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6480, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6801", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6480", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ffce11d-944a-47c3-bde6-f34778f3433a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6479, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006479__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6480-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6480, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6481, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6802", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6481", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a3a0b57-700a-45ad-8986-e44f5e9caa22", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6480, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006480__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6481-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6481, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6482, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6803", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6482", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ff1fba5-a3f5-4b37-8f1a-1f79c3ca9ead", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6481, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006481__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6482-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6482, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6483, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6804", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6483", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba866ac3-f0d3-44b1-b61c-c57727545757", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6482, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006482__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6483-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6483, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6484, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6805", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6484", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d7392b5-eb19-4471-93e2-f16a89a51872", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6483, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006483__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6484-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6484, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6485, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6806", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6485", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da0ae164-2c77-4728-80d6-c97f9a280175", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6484, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006484__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6485-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6485, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6486, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6807", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6486", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88d65695-708e-4ce6-b8a3-1ab0308b4882", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6485, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006485__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6486-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6486, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6487, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6808", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6487", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bba21682-80d4-4a62-b166-d1499f635076", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6486, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006486__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6487-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6487, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6488, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6809", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6488", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19bdebae-5dc7-4e13-9e50-17e2ec155f89", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 562971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6487, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006487__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6488-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6488, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6489, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6810", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6489", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40609b45-8660-45e4-b46b-6c7fbb5008d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6488, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006488__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6489-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6489, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "562937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6490, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6811", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6490", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1bf8e6da-14d6-4000-985f-6af500c8f411", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6489, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006489__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6490-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6490, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6491, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6812", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6491", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a58c12c3-1880-4105-a1b8-963fde76aa97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6490, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006490__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6491-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6491, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6492, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6813", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6492", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f0abc7e9-72e3-47a9-83b8-309b6f67ff4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6491, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006491__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6492-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6492, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6493, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6814", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6493", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fbc1b4b-c4c7-48fb-a3f2-4ce4f0a90a94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6492, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006492__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6493-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6493, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6494, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6815", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6494", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b60cf84b-ca7f-4914-854b-f2d683429ad5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6493, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006493__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6494-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6494, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6495, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6816", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6495", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a0da01f-7dd8-487c-a399-3abf74c2bf51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6494, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006494__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6495-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6495, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6496, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6817", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6496", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "423008a0-f9e8-49a0-ab5c-d8eaf8103c68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6495, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006495__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6496-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6496, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6497, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6818", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6497", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3658833b-2956-485c-b9d2-eefe2d52cfc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6496, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006496__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6497-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6497, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6498, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6819", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6498", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16e8a9d5-6908-4da6-9b5a-0d3e036d15cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6497, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006497__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6498-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6498, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6499, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6820", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6499", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24b23cee-7b7c-46fe-9b55-edc2df8ad261", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 563940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6498, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006498__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6499-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6499, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6500, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6821", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6500", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67ab6ac3-d2a7-479f-a394-59f8f73598a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6499, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006499__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6500-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6500, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6501, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6822", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6501", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12104145-b4c7-451b-b75c-5507a5502bfe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6500, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006500__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6501-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6501, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "563968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6502, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6823", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6502", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "caa978bc-ff65-4010-b274-f1a94e065733", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6501, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006501__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6502-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6502, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6503, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6824", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6503", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d22c9b53-1664-46cd-a561-8207cf9765a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6502, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006502__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6503-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6503, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6504, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6825", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6504", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d8fe8e2b-29f5-488c-831d-7810784461a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6503, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006503__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6504-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6504, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6505, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6826", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6505", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9b928d5-f56d-461b-b3c7-e396b3c99031", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6504, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006504__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6505-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6505, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6506, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6827", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6506", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a4f17a4-fbf6-4a1a-92d7-3a16fd6f4c20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6505, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006505__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6506-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6506, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6507, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6828", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6507", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53c1c74d-cf0a-4aac-ba7d-a69b328815de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6506, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006506__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6507-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6507, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6508, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6829", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6508", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1206d49f-c514-4c1c-8efa-fd0b9977ff13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6507, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006507__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6508-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6508, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6509, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6830", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6509", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f10aa75c-99d6-4a88-bb62-045814a8be38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6508, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006508__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6509-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6509, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6510, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6831", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6510", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "057b5409-9fbb-48df-99d2-623862f70c4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6509, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006509__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6510-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6510, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6511, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6832", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6511", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cd51ea2-54e6-4a71-b281-c3a7edec414d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 564971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6510, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006510__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6511-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6511, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6512, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6833", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6512", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae55ef35-5684-4268-b04f-b09c63f8e702", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6511, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006511__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6512-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6512, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "564921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6513, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6834", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6513", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e04c4b6c-b7a9-4fa8-b9ef-7f7534d016b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6512, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006512__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6513-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6513, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6514, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6835", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6514", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94032cfe-be48-410e-89e0-6b3e5af003ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6513, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006513__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6514-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6514, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6515, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6836", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6515", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4db7e073-6d42-458b-a4de-6f7f768293dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6514, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006514__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6515-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6515, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6516, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6837", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6516", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e754300-58a7-4531-abb7-684eabac0079", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6515, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006515__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6516-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6516, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6517, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6838", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6517", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cbb18a62-e601-45e6-b94f-890ba97849f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6516, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006516__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6517-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6517, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6518, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6839", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6518", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "919ce1e8-af29-458b-8b13-4a98dfe4be4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6517, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006517__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6518-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6518, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6519, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6840", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6519", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0ae8e3e-43cc-4341-8b4a-2e69369913fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6518, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006518__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6519-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6519, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6520, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6841", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6520", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9429e617-e407-4256-b2ef-b0fd979fe8f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6519, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006519__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6520-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6520, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6521, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6842", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6521", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab937e6e-8f9c-4119-80af-0d123a76e674", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6520, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006520__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6521-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6521, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6522, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6843", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6522", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b000ef8e-e069-4981-be24-24ce21589062", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 565924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6521, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006521__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6522-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6522, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6523, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6844", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6523", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "209255dc-13d9-4723-a98e-fb0e00d97b46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6522, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006522__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6523-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6523, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6524, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6845", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6524", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed2eb9bb-2440-4cdc-90a4-0b1464de23f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6523, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006523__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6524-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6524, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "565968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6525, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6846", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6525", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc0300f4-4c02-4fa3-9003-ad8badfab41f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6524, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006524__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6525-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6525, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6526, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6847", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6526", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73819d67-4d1e-4b82-ab80-55e5b2b2b1be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6525, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006525__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6526-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6526, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6527, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6848", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6527", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e2e3c9ba-9dee-474e-9437-40659b03e12c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6526, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006526__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6527-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6527, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6528, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6849", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6528", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "485fa78e-912c-4299-8e8c-d207b5bc743f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6527, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006527__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6528-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6528, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6529, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6850", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6529", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8fefa76-94d4-4637-972f-8c1e38d2ac20", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6528, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006528__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6529-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6529, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6530, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6851", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6530", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b64485d0-443c-4e4a-a097-b0b370cb68f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6529, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006529__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6530-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6530, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6531, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6852", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6531", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9642a8cd-ef26-462a-80f8-d883d5b95b77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6530, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006530__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6531-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6531, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6532, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6853", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6532", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab80c012-6727-46e5-bfa1-ff05a071ca8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6531, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006531__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6532-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6532, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6533, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6854", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6533", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bbbe6db-e829-4ccd-af7f-88799bc45f4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6532, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006532__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6533-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6533, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6534, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6855", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6534", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecd070a1-3b66-4461-b152-569587ea5cfe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 566971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6533, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006533__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6534-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6534, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6535, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6856", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6535", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7dbb96dd-4d6d-413b-834d-c80d48c37bf5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6534, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006534__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6535-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6535, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "566921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6536, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6857", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6536", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f61fd88b-4cf8-403b-a1e9-d09eff1522d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6535, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006535__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6536-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6536, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6537, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6858", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6537", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d6dabd5-88ac-4282-8efa-a66f046dd93d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6536, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006536__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6537-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6537, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6538, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6859", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6538", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdfa14a4-f582-4881-8f77-8eaef4308caf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6537, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006537__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6538-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6538, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6539, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6860", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6539", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7bb9bb12-08b6-48f4-965e-eebd05b30b26", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6538, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006538__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6539-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6539, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6540, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6861", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6540", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a860df1c-3562-456c-95a7-6afc9de7d587", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6539, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006539__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6540-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6540, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6541, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6862", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6541", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a606cb7-5ef4-489b-91f6-9aa6ed03dd28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6540, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006540__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6541-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6541, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6542, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6863", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6542", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e85f9c92-348f-4a40-88f8-a805f11b6828", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6541, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006541__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6542-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6542, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6543, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6864", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6543", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4559493c-5f3f-449f-bb3b-84a7eb68024a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6542, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006542__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6543-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6543, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6544, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6865", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6544", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d48db05-8377-4c1d-bca8-f2380e892376", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6543, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006543__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6544-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6544, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6545, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6866", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6545", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "278a2dd8-2666-4421-9abd-d88cd715dea4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 567924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6544, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006544__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6545-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6545, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6546, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6867", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6546", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52700a0a-4024-4ed0-88c1-8fac7c0338ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6545, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006545__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6546-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6546, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6547, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6868", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6547", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "168fa98b-5871-4573-be11-67f354b02e63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6546, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006546__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6547-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6547, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "567968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6548, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6869", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6548", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b24723b-c4e4-4836-acdd-bbaf14bbf648", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6547, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006547__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6548-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6548, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6549, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6870", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6549", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "370232b6-2b7c-49e9-981a-6b7ba391976d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6548, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006548__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6549-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6549, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6550, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6871", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6550", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0955206e-7688-4607-984c-9a8957d00c15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6549, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006549__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6550-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6550, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6551, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6872", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6551", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09caa876-5712-4d9f-b99e-028c7825568f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6550, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006550__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6551-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6551, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6552, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6873", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6552", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6ae0bca-8f74-4c7e-ba44-6d9ec1fbf741", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6551, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006551__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6552-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6552, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6553, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6874", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6553", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9118d232-e413-4723-b642-85b75e9fd7f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6552, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006552__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6553-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6553, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6554, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6875", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6554", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a088ef51-85b6-4db1-b1cb-5f309ab3bea0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6553, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006553__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6554-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6554, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6555, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6876", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6555", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a4268f2-dab2-4346-90b8-a06b0b9fd5dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6554, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006554__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6555-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6555, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6556, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6877", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6556", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e2715d8-6ccf-4cae-8aa8-896bad64ee8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6555, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006555__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6556-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6556, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6557, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6878", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6557", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5abea3e-738d-45a8-97cc-bf6bebfd7eed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 568971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6556, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006556__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6557-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6557, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6558, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6879", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6558", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c26ced29-c80c-4492-b4d9-e2130818fa7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6557, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006557__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6558-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6558, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "568921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6559, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6880", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6559", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "daf75fce-9ffc-40b1-9f68-7390c5e0931b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6558, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006558__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6559-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6559, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6560, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6881", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6560", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c337fac6-138c-4a07-84cc-66202f77cab6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6559, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006559__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6560-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6560, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6561, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6882", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6561", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7bc78d80-6811-40e8-82b9-ad7a9b147a07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6560, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006560__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6561-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6561, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6562, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6883", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6562", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2acfb69-7463-4192-bb8e-d760bd807aae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6561, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006561__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6562-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6562, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6563, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6884", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6563", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65c9ae8a-449e-45ce-99ce-f3406e476d38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6562, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006562__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6563-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6563, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6564, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6885", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6564", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac48edea-e1c7-4c82-bdcc-8ab810c48f2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6563, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006563__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6564-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6564, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6565, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6886", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6565", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e1eb9e3-947f-4d8c-a76e-7d6519977cfd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6564, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006564__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6565-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6565, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6566, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6887", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6566", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5edb1708-6522-42a3-9d13-fd9bb59f5cbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6565, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006565__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6566-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6566, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6567, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6888", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6567", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e07a3794-5442-4653-a715-07e8d939f9f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6566, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006566__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6567-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6567, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6568, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6889", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6568", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e950964-d83a-402d-9d0e-1f40c89cb341", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 569924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6567, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006567__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6568-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6568, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6569, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6890", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6569", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "566b69cb-205c-4059-a912-7c297acb7357", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6568, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006568__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6569-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6569, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6570, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6891", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6570", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc45ddfd-c90b-426a-a27f-a900f02b1433", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6569, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006569__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6570-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6570, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "569953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6571, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6892", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6571", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d214a64d-95ee-4b99-a3e0-627f19ff96da", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6570, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006570__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6571-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6571, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6572, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6893", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6572", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "655f1c9f-c24d-4cf5-860f-d1a366091422", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6571, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006571__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6572-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6572, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6573, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6894", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6573", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04c319a2-ad90-4f78-b102-e8e44daf85ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6572, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006572__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6573-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6573, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6574, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6895", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6574", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaa06ab2-e9df-4c24-8f31-0f55409283bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6573, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006573__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6574-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6574, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6575, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6896", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6575", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bb87c28-e35a-4022-9fcc-3afa54ddaccf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6574, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006574__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6575-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6575, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6576, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6897", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6576", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1749a1a-4115-40b0-b914-efde2a9dcc0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6575, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006575__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6576-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6576, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6577, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6898", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6577", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f226d66-2cbf-4d89-afd8-4eb6704b8e2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6576, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006576__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6577-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6577, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6578, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6899", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6578", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f26b60d-a872-4a82-83d1-7e52731ab686", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6577, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006577__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6578-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6578, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6579, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6900", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6579", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "690a2ab9-82f8-4093-8091-114c0db67080", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6578, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006578__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6579-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6579, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6580, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6901", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6580", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c038014-62b7-4d28-b001-3bdefd6bb4a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 570955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6579, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006579__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6580-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6580, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6581, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6902", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6581", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fbb32c6a-7287-4ebc-9b1a-dd9dd5b15ab8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6580, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006580__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6581-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6581, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "570906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6582, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6903", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6582", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e75e87f-bc3a-42a1-b62c-8e82d4e96f84", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6581, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006581__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6582-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6582, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6583, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6904", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6583", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "842c04ce-4c5b-4fbe-903c-cf6cd5fb08c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6582, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006582__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6583-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6583, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6584, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6905", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6584", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "207b8945-d4ac-4efa-be16-8e9c59bc224e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6583, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006583__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6584-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6584, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6585, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6906", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6585", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14ec3ef0-1025-4794-93f0-6788cc669b08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6584, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006584__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6585-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6585, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6586, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6907", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6586", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48fec618-d48b-449c-827e-add32d29a289", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6585, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006585__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6586-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6586, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6587, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6908", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6587", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3878a04e-6fc7-4dfb-b2ec-d8caed441181", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6586, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006586__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6587-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6587, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6588, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6909", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6588", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fde55d1d-b6c7-4766-9f4b-957fb95c5fcb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6587, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006587__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6588-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6588, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6589, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6910", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6589", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a0d498f-9ca7-441f-8feb-cc1253ab65f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6588, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006588__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6589-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6589, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6590, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6911", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6590", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2f44fe6-d88b-4dc2-9c4f-e3c78e445938", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6589, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006589__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6590-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6590, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6591, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6912", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6591", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1c15f4d5-71bc-45a5-aa15-5e3e0671b53b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6590, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006590__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6591-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6591, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6592, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6913", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6592", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abe723bc-d7be-4c3a-863f-1aed303cec5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6591, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006591__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6592-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6592, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6593, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6914", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6593", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0662d04-0c56-4431-a043-f0d5c5eea263", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6592, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006592__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6593-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6593, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "571953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6594, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6915", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6594", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf1bf83e-8083-4e19-986a-7138004c8323", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6593, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006593__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6594-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6594, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6595, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6916", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6595", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12a3501f-e9ed-4c6d-b10f-a967ad335701", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6594, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006594__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6595-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6595, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6596, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6917", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6596", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66366bba-b2f0-4d19-99c6-68d795ee4653", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6595, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006595__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6596-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6596, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6597, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6918", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6597", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adac65ab-cac2-4d4a-be9b-ebc66a47dcb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6596, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006596__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6597-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6597, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6598, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6919", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6598", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1ca6dd6-058a-48d0-a866-a6e497969725", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6597, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006597__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6598-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6598, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6599, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6920", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6599", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3f508af-bea0-4cd6-a0ec-cf8ebb43fdc9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6598, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006598__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6599-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6599, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6600, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6921", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6600", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "251b83f7-87de-4031-92da-a9b0ba8ee48a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6599, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006599__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6600-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6600, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6601, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6922", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6601", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ac3f98f9-3c87-4c46-a73a-2fd23fae423c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6600, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006600__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6601-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6601, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6602, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6923", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6602", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2401a95-07a8-487a-b811-288329eb54e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6601, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006601__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6602-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6602, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6603, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6924", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6603", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e90c66a-4799-422d-bc1b-9d1d3fe0412b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 572955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6602, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006602__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6603-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6603, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6604, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6925", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6604", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23618cce-36b5-480b-881f-4173d36d27e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6603, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006603__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6604-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6604, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "572906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6605, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6926", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6605", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e38723f-b8da-43c3-a550-d940c5ed9a21", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6604, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006604__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6605-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6605, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6606, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6927", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6606", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81351edf-c0b2-48eb-9831-a180b2c69d95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6605, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006605__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6606-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6606, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6607, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6928", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6607", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88ac57ff-d17b-45f4-95de-5ceb9388d238", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6606, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006606__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6607-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6607, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6608, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6929", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6608", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f171d61-9139-4875-a4a7-c03314109ae0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6607, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006607__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6608-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6608, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6609, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6930", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6609", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6ba5287-be58-4f88-8ade-1d76d93d7673", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6608, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006608__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6609-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6609, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6610, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6931", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6610", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53c292be-659b-421e-8902-4472771015a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6609, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006609__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6610-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6610, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6611, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6932", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6611", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83703d15-fd40-44e3-adb8-42f8b87f73a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6610, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006610__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6611-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6611, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6612, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6933", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6612", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c34203c0-3fb0-4514-afe0-664905f4e8c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6611, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006611__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6612-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6612, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6613, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6934", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6613", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3389bb26-6f2e-43a2-8785-7b736ab04546", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6612, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006612__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6613-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6613, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6614, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6935", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6614", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c40c9c5-76f0-419b-ad9e-9d9af5c54081", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6613, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006613__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6614-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6614, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6615, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6936", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6615", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b02264d-b8c4-467d-9429-4363ffa98645", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 573986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6614, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006614__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6615-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6615, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6616, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6937", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6616", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be7fc0fd-931c-4905-a45e-d3494816008a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6615, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006615__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6616-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6616, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "573953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6617, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6938", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6617", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f911fb8c-5906-4d9a-9a29-80a83723b436", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6616, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006616__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6617-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6617, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6618, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6939", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6618", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "710a06d9-2245-4d40-a8bd-c08d39f83ebc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6617, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006617__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6618-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6618, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6619, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6940", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6619", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b2fbca9-ab4e-4fd2-b5fb-d92aade69b0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6618, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006618__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6619-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6619, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6620, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6941", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6620", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22d872e7-7c94-4451-9355-fa682969442b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6619, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006619__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6620-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6620, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6621, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6942", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6621", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e773923-0e9b-455b-b15e-e8b0af886614", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6620, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006620__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6621-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6621, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6622, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6943", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6622", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "93cf8513-df5b-43f2-bfe6-f682a0ca69d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6621, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006621__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6622-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6622, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6623, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6944", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6623", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d8c9242-b376-43a9-9df0-0d0ec2575936", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6622, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006622__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6623-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6623, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6624, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6945", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6624", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f37b96c-8d43-4a9e-a918-f736902fbd1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6623, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006623__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6624-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6624, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6625, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6946", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6625", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "570cb9ab-48f3-4422-a6a1-ae103315327b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6624, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006624__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6625-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6625, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6626, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6947", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6626", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8868b7a4-aff1-40a5-be9d-67fb62165ecc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 574955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6625, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006625__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6626-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6626, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6627, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6948", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6627", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7255bac-af3e-4d86-8835-ac648898773d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6626, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006626__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6627-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6627, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6628, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6949", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6628", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "524169be-dcaa-40ca-9473-97eede72f80b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6627, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006627__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6628-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6628, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "574984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6629, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6950", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6629", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d96c3d81-5002-41e0-99a7-7fcd928ed2b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6628, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006628__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6629-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6629, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6630, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6951", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6630", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "664510c5-8ca1-4ae1-ad0c-f45e5c759932", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6629, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006629__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6630-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6630, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6631, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6952", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6631", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2fcf8f29-a367-46e4-9ae5-9f7f79264a0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6630, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006630__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6631-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6631, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6632, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6953", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6632", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1328bbdb-7d6c-444e-ba13-2e1640846ec8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6631, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006631__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6632-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6632, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6633, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6954", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6633", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7986295f-89f9-48e8-86fe-867e38a6ed62", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6632, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006632__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6633-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6633, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6634, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6955", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6634", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42b2d6b1-a25e-4809-90ee-28e47dc0a14c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6633, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006633__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6634-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6634, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6635, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6956", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6635", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9863fa5-bfe2-447c-8892-b948fd7ef2e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6634, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006634__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6635-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6635, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6636, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6957", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6636", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdf2f2c3-f951-42d9-ae19-4eb9285cef32", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6635, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006635__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6636-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6636, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6637, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6958", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6637", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4dcaa2f-cc2b-4483-9d0b-aa5e008d9bb2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6636, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006636__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6637-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6637, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6638, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6959", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6638", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dc8e1869-5d55-4b77-b76b-5dfbe47237db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 575986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6637, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006637__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6638-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6638, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6639, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6960", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6639", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "528f1ac6-8431-4fab-8fae-2b0fc708e30b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6638, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006638__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6639-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6639, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "575937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6640, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6961", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6640", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "666dc695-362c-4476-b7a0-0fed91d43f69", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6639, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006639__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6640-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6640, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6641, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6962", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6641", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "756b7f1d-ef91-465a-bb40-55e20575a36b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6640, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006640__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6641-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6641, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6642, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6963", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6642", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "137239bf-e33f-4a9c-9250-5c2490b33fd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6641, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006641__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6642-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6642, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6643, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6964", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6643", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b596a9d2-ddc1-4035-966b-d90b062d30e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6642, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006642__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6643-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6643, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6644, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6965", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6644", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81f38bf3-a7a8-45bf-8f5e-3bdbc50ae9bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6643, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006643__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6644-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6644, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6645, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6966", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6645", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e589f5c4-67df-4af0-8c37-1c6d441a22db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6644, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006644__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6645-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6645, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6646, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6967", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6646", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08198abe-006b-4949-ae24-afe554200243", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6645, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006645__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6646-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6646, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6647, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6968", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6647", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "365862f7-2705-40c6-a89b-eaee98d800ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6646, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006646__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6647-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6647, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6648, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6969", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6648", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "315a1023-79a5-4927-95a1-84448b0e0745", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6647, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006647__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6648-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6648, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6649, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6970", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6649", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f29d2424-94b0-401e-ab5e-557ca4fca85b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 576940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6648, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006648__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6649-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6649, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6650, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6971", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6650", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6922e10d-3cbb-4f87-ae78-18b54992edd7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6649, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006649__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6650-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6650, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6651, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6972", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6651", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2b6a665d-411f-4ccf-9f40-7f32ccb231cf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6650, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006650__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6651-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6651, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "576984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6652, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6973", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6652", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff0da032-92de-48c3-a9d6-dd6f65ea541c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6651, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006651__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6652-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6652, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6653, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6974", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6653", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd3d2260-b8a2-4e6d-a1a7-bc74b987a463", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6652, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006652__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6653-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6653, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6654, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6975", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6654", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11e01846-5cf3-4041-83dc-85fe6f8a0d39", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6653, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006653__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6654-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6654, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6655, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6976", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6655", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "021a4e1d-7585-43d7-a685-0c748b29ef38", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6654, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006654__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6655-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6655, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6656, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6977", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6656", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fe54b05-a34f-4bb8-8e95-d71a665995ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6655, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006655__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6656-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6656, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6657, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6978", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6657", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b52c6cb4-863d-4e63-9373-d91fff7bc583", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6656, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006656__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6657-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6657, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6658, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6979", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6658", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6220935-e65c-48ec-bc3d-16ae2c036438", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6657, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006657__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6658-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6658, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6659, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6980", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6659", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60d5fab7-bf79-4d5b-b670-7698803f37f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6658, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006658__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6659-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6659, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6660, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6981", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6660", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e112f57-359b-435b-b8a8-f5642a5363c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6659, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006659__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6660-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6660, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6661, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6982", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6661", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "661809f8-666c-47e2-a7a3-87e45a4f51c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 577986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6660, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006660__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6661-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6661, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6662, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6983", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6662", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e605456f-1457-401a-808c-d2e73322f988", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6661, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006661__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6662-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6662, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "577937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6663, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6984", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6663", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38d18a91-76fc-4c69-8d8f-058e474c3aaa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6662, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006662__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6663-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6663, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6664, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6985", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6664", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "879df987-8278-466c-bd01-991944f1ae5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6663, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006663__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6664-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6664, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6665, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6986", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6665", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca50a8af-3d5f-4ff0-8c6f-612d859d693e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6664, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006664__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6665-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6665, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6666, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6987", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6666", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1cacc503-1712-4a6e-852a-c720aa722611", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6665, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006665__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6666-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6666, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6667, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6988", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6667", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c64b3bf-e379-4f78-afb9-e56da2f801ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6666, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006666__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6667-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6667, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6668, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6989", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6668", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc9876ed-f6e4-42a6-9722-69b4a7b7afef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6667, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006667__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6668-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6668, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6669, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6990", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6669", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37adbecc-dd17-4ef1-a8e2-65012a360c64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6668, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006668__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6669-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6669, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6670, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6991", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6670", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5b1954a-11b6-4989-8937-07b28e73621b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6669, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006669__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6670-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6670, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6671, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6992", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6671", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f8b9cee-1644-47ed-b04f-ee89e086acf0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6670, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006670__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6671-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6671, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6672, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6993", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6672", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "713739d5-9a5d-4e66-896d-f1dd21fb13a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 578940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6671, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006671__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6672-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6672, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6673, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6994", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6673", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6c74d6c-ba87-4d0c-9e32-037b8537c352", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6672, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006672__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6673-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6673, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6674, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6995", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6674", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ec48076-acc3-449f-9d8a-1acf0c78f747", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6673, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006673__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6674-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6674, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "578984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6675, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6996", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6675", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0669565c-70aa-4a62-90d2-607357f2cba1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6674, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006674__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6675-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6675, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6676, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6997", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6676", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "167e4fb0-dc98-4e1b-9a2d-d4544a0562c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6675, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006675__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6676-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6676, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6677, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6998", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6677", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "287c52ae-9642-48cc-baaf-90011d406055", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6676, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006676__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6677-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6677, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6678, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "6999", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6678", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00c35e3d-d852-478a-ab10-6699c6730ce2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6677, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006677__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6678-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6678, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6679, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7000", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6679", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a775f7f8-9a3e-40fc-bb72-e966102c677a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6678, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006678__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6679-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6679, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6680, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7001", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6680", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c7e061b-e0db-4583-aa20-463093433d86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6679, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006679__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6680-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6680, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6681, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7002", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6681", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3059625-2e76-4121-97c8-ea19ff815203", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6680, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006680__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6681-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6681, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6682, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7003", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6682", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d38dd5b0-135c-4f9c-9013-c6e95317c219", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6681, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006681__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6682-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6682, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6683, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7004", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6683", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc894d03-53f1-42d6-8124-9a2b08987573", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6682, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006682__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6683-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6683, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6684, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7005", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6684", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ccb76bd-4d4f-44c8-bd3e-301ee5748c55", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 579986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6683, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006683__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6684-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6684, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6685, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7006", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6685", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70f701cb-1ab0-410c-bd2a-c95ebb340ab1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6684, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006684__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6685-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6685, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "579937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6686, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7007", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6686", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfd66e97-0395-4198-aaa1-55e01918af83", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6685, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006685__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6686-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6686, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6687, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7008", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6687", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b988566b-d199-4849-9760-6b99ff706f13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6686, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006686__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6687-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6687, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6688, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7009", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6688", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a6b8297-975a-4e2c-81c1-3fa455265ea7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6687, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006687__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6688-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6688, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6689, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7010", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6689", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a75e1110-380b-468b-96ce-0fbf0cde7a05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6688, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006688__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6689-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6689, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6690, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7011", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6690", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd78aa43-0192-4c2b-9ca5-4a61e02d0700", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6689, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006689__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6690-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6690, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6691, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7012", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6691", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c832999-b7d2-44b4-b524-fdd52ca2bd5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6690, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006690__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6691-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6691, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6692, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7013", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6692", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72de70d5-a633-4496-9499-944f78072931", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6691, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006691__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6692-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6692, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6693, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7014", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6693", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7151904c-6d02-4011-9cc0-8a32eebc13dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6692, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006692__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6693-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6693, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6694, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7015", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6694", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ffaad09-53d5-4bf0-9e01-83a0a6289076", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6693, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006693__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6694-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6694, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6695, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7016", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6695", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9fd6a27-53a5-4d4e-857f-099772ea5845", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 580940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6694, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006694__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6695-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6695, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6696, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7017", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6696", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92ac9766-ae99-4f43-827e-aad3a9e61a04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6695, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006695__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6696-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6696, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6697, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7018", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6697", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b70aa3a-c19f-4f69-88c7-709b20b978a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6696, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006696__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6697-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6697, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "580968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6698, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7019", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6698", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0cad0ee-7067-4bc2-8f14-03838c4d7681", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6697, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006697__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6698-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6698, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6699, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7020", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6699", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "345d2fbc-a054-4cc3-a876-2d15ad5a893e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6698, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006698__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6699-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6699, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6700, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7021", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6700", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d25f4d2-cee8-4814-963c-ca7e8df6e89b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6699, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006699__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6700-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6700, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6701, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7022", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6701", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1ff2758-ef6a-44f2-9f26-6c7af1280c2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6700, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006700__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6701-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6701, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6702, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7023", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6702", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8965cc3f-2bc0-4c52-a114-b6b6e571cd8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6701, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006701__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6702-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6702, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6703, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7024", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6703", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85398b4a-8be1-4859-8170-83a50c1cf40e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6702, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006702__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6703-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6703, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6704, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7025", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6704", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a541f03c-6d2b-4c72-82bc-4c5f4d05aa1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6703, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006703__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6704-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6704, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6705, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7026", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6705", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cb34640-fdd8-4b71-b383-ffe1137e17bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6704, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006704__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6705-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6705, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6706, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7027", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6706", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfefab35-abd9-4f14-8058-3cff49e4b972", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6705, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006705__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6706-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6706, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6707, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7028", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6707", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aeabae6e-2659-4033-8fda-1488ff936e50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 581971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6706, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006706__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6707-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6707, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6708, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7029", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6708", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "178d36b1-6dac-4158-96e6-c0098fb5b3a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6707, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006707__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6708-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6708, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "581921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6709, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7030", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6709", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3b16308-3d57-4515-97cc-989bdc9abe0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6708, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006708__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6709-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6709, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6710, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7031", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6710", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf5c5ac5-f3e4-42d4-9574-39256211de4b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6709, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006709__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6710-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6710, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6711, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7032", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6711", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58e8f6e6-a2ee-4046-8232-8588dfe9ad45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6710, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006710__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6711-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6711, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6712, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7033", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6712", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "801ce2bb-b237-4803-b75b-0a66c98e7b96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6711, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006711__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6712-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6712, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6713, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7034", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6713", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e55f530-bf76-460a-af96-74742ea5806e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6712, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006712__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6713-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6713, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6714, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7035", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6714", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c5dea289-7b2b-4d36-bbe1-2bb2ea01f54c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6713, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006713__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6714-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6714, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6715, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7036", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6715", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cee10122-0525-4190-8225-f2f3a0306f76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6714, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006714__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6715-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6715, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6716, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7037", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6716", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1216c0f1-4298-4636-b571-be498547b7cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6715, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006715__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6716-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6716, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6717, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7038", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6717", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4993d1d-12c2-41ec-90da-4dcd96d7ae7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6716, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006716__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6717-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6717, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6718, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7039", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6718", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "663d7065-cf21-4227-b890-7094d30df153", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 582924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6717, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006717__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6718-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6718, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6719, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7040", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6719", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ede8282-aa14-475b-a2e3-9c56cb5e8221", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6718, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006718__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6719-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6719, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6720, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7041", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6720", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2d88953-137f-433e-82ee-85d2e9f7936b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6719, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006719__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6720-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6720, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "582968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6721, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7042", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6721", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bfebcd2-ab25-4e5a-8902-8a56f496be17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6720, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006720__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6721-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6721, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6722, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7043", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6722", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e01fbce2-eb5a-4b3b-9370-6d0cca18c825", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6721, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006721__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6722-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6722, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6723, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7044", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6723", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a153582-69fc-4aee-9536-ca7a47f56b4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6722, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006722__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6723-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6723, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6724, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7045", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6724", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c53035e0-b079-4bad-987f-d9746cb9b97d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6723, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006723__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6724-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6724, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6725, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7046", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6725", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a514672-aad4-40fa-b07c-9f36970d8d86", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6724, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006724__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6725-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6725, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6726, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7047", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6726", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bfa49078-b59e-4bde-b77f-85d167d06e33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6725, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006725__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6726-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6726, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6727, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7048", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6727", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "646d9782-a038-4460-bc7c-8461cb2313eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6726, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006726__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6727-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6727, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6728, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7049", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6728", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22500f6a-4bec-420a-a8a0-bd89a349765c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6727, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006727__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6728-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6728, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6729, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7050", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6729", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0346e25-b0e8-4b2a-a452-5809e016bb2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6728, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006728__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6729-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6729, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6730, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7051", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6730", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a194a1f-9b7a-4b0e-87f7-fc0bd4441c1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 583971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6729, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006729__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6730-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6730, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6731, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7052", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6731", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "381b8c1e-05a7-4b19-ada8-d683e7da9100", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6730, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006730__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6731-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6731, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "583921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6732, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7053", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6732", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8553327d-b339-4fa1-8113-75bed3320a31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6731, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006731__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6732-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6732, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6733, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7054", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6733", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bae944f7-2e9b-4d6e-ae8d-d28b4a0a7e08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6732, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006732__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6733-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6733, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6734, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7055", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6734", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cb5cb70-1a89-48b6-9078-ca7d77885f9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6733, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006733__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6734-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6734, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6735, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7056", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6735", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23155442-c117-4538-a8d7-7cbe610cddee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6734, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006734__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6735-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6735, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6736, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7057", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6736", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a478efd0-4cf0-4c98-b29e-c1591be79e6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6735, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006735__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6736-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6736, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6737, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7058", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6737", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ce9be1b-8eae-4b55-a8bb-54a88deb2ae2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6736, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006736__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6737-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6737, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6738, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7059", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6738", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cdb025ee-50e9-4fdb-a3fb-688d18f39be2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6737, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006737__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6738-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6738, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6739, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7060", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6739", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24cd07fe-4cd7-4800-bb94-14fd4c563839", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6738, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006738__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6739-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6739, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6740, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7061", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6740", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f7fea34-eed1-4c71-8267-32c884ce059b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6739, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006739__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6740-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6740, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6741, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7062", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6741", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b3a1826c-a47f-4994-9620-4d313f8d2a90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 584924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6740, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006740__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6741-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6741, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6742, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7063", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6742", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6755f5d2-478b-4930-affb-89e05700f730", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6741, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006741__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6742-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6742, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6743, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7064", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6743", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68267478-8b03-4d59-ba18-da982bc2b430", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6742, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006742__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6743-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6743, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "584968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6744, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7065", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6744", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a36d822-c1dd-4e6f-87a8-6a9e8f0663f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6743, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006743__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6744-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6744, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6745, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7066", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6745", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea9ce1ab-588a-47ce-a6be-46425dddf13c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6744, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006744__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6745-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6745, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6746, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7067", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6746", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aec3fb75-dbeb-4ff9-adae-587baf5a668d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6745, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006745__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6746-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6746, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6747, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7068", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6747", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef6c6abe-abb6-4e67-9e51-f41b95428f9e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6746, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006746__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6747-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6747, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6748, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7069", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6748", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "366ce546-0a57-474e-a2c4-6c88d0b5e4f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6747, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006747__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6748-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6748, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6749, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7070", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6749", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ccfbf64-53af-4c0c-afdd-8c7f49db31a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6748, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006748__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6749-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6749, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6750, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7071", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6750", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f37a769b-5f64-4bef-b2ab-b756d23173ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6749, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006749__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6750-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6750, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6751, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7072", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6751", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5f4a6f6-2eb7-4b5b-af69-e720dc0adcd4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6750, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006750__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6751-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6751, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6752, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7073", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6752", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47bdeb42-85bd-4e45-8f89-5b97bcbf8b8d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6751, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006751__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6752-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6752, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6753, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7074", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6753", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67dd0792-0709-498b-b1a9-caff44455b6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 585971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6752, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006752__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6753-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6753, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6754, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7075", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6754", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5986d3e-6fe5-4071-81b3-6926aee142c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6753, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006753__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6754-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6754, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "585921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6755, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7076", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6755", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "768aa46b-1740-42fa-8a45-9bbe7e1cccc4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6754, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006754__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6755-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6755, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6756, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7077", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6756", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "41911125-fdc4-4812-85b6-d9a0fc0a9f48", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6755, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006755__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6756-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6756, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6757, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7078", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6757", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64895968-11f5-43a7-ada0-fe641d7a5c5e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6756, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006756__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6757-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6757, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6758, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7079", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6758", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86b490fb-0b54-491c-b63b-7411b4dce6e9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6757, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006757__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6758-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6758, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6759, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7080", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6759", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0baacbe3-ab6e-4a1f-86e7-3a64c27e6d92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6758, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006758__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6759-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6759, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6760, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7081", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6760", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8e8f857-180b-45a1-ba86-954e82a4c808", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6759, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006759__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6760-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6760, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6761, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7082", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6761", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cee72809-3ed6-4c6c-b562-d98e549cce2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6760, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006760__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6761-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6761, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6762, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7083", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6762", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3db287a3-aede-4237-9763-2a7b0610b157", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6761, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006761__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6762-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6762, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6763, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7084", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6763", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8d4d351-dfec-4b41-9c19-42e6f1ee0523", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6762, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006762__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6763-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6763, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6764, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7085", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6764", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "138fed54-d285-4e27-90b1-4ac56f44d936", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 586924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6763, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006763__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6764-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6764, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6765, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7086", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6765", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07d4b15d-6e3a-4e1f-9da9-18ca5759dac4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6764, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006764__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6765-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6765, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6766, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7087", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6766", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d33cec6-b0c6-4057-9487-dee382fd323f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6765, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006765__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6766-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6766, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "586953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6767, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7088", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6767", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14149173-1d55-401e-8041-d560abebf836", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6766, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006766__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6767-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6767, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6768, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7089", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6768", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32c0c2e6-d2fa-4f1e-b438-6f95466c89f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6767, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006767__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6768-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6768, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6769, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7090", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6769", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae043a80-f1b4-43d5-a564-6083b9f2ed06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6768, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006768__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6769-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6769, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6770, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7091", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6770", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8bb89ac8-7972-4bd3-81f5-fe52bd9f3dd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6769, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006769__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6770-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6770, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6771, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7092", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6771", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a07d7639-b683-4171-a3d9-a1fa71ea36b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6770, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006770__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6771-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6771, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6772, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7093", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6772", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "900d4227-5501-460e-96d6-835c5a4d85b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6771, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006771__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6772-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6772, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6773, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7094", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6773", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbeef396-ccfa-4ee0-8c95-fbdb5c9cedeb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6772, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006772__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6773-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6773, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6774, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7095", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6774", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "854c7890-1f81-4e47-b6c8-aea7f233ea24", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6773, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006773__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6774-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6774, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6775, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7096", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6775", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d297fdf2-513d-42f7-b5dc-e19d7c068fd4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6774, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006774__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6775-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6775, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6776, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7097", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6776", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e6451ff-8fcf-4897-ae2c-692c2dcb0443", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 587955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6775, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006775__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6776-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6776, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6777, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7098", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6777", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ac1f339-0747-44f1-aa0e-f3c4caf92bf2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6776, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006776__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6777-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6777, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "587906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6778, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7099", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6778", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3766a738-b1fa-4d11-ae1e-856d25a82097", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6777, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006777__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6778-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6778, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6779, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7100", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6779", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1843e8c3-f8f2-4dc8-985c-6add121e6d97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6778, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006778__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6779-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6779, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6780, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7101", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6780", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ad038e1-3dc3-4de1-90d2-789087126e2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6779, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006779__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6780-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6780, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6781, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7102", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6781", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a69ae062-57f6-43bd-b583-0f6bb8b4b139", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6780, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006780__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6781-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6781, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6782, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7103", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6782", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71f875cb-552f-499c-bae3-98be6748ac58", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6781, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006781__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6782-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6782, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6783, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7104", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6783", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "298be3e2-daac-4321-ad0e-68c3a54a15ef", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6782, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006782__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6783-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6783, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6784, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7105", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6784", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0345f170-2de3-4453-b6b4-5e7a0ec0b745", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6783, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006783__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6784-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6784, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6785, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7106", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6785", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7401d752-95c5-445c-9b36-f97c28c04c4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6784, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006784__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6785-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6785, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6786, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7107", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6786", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0961b23f-6ab0-4f2a-b41f-c526aa6eecf2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6785, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006785__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6786-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6786, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6787, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7108", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6787", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d50cbf04-5e97-4460-abeb-b8ea8dc16896", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 588908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6786, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006786__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6787-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6787, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6788, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7109", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6788", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5dd06d5-6786-40ae-9647-96316e22ec34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6787, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006787__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6788-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6788, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6789, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7110", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6789", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3720dac1-686e-4ec6-96d8-9919210cf2d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6788, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006788__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6789-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6789, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "588953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6790, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7111", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6790", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f88e6f1-a8ab-4365-b896-c41905f544ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6789, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006789__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6790-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6790, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6791, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7112", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6791", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ec021c0-a9e2-4590-999e-6f1337bb92de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6790, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006790__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6791-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6791, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6792, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7113", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6792", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b34cd3c-6dd2-4e0f-89e5-84a883e74323", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6791, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006791__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6792-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6792, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6793, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7114", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6793", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c66b4ea9-f27d-4efc-9015-1f6e0e28a993", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6792, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006792__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6793-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6793, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6794, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7115", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6794", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9608358-0682-4ffc-8088-cbda1bd75351", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6793, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006793__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6794-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6794, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6795, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7116", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6795", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ebdfd15-9d17-4aa6-a0e7-4c5ef77d1a7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6794, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006794__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6795-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6795, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6796, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7117", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6796", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42804778-a5fc-4d08-a499-dd386cf9472d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6795, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006795__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6796-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6796, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6797, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7118", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6797", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d1d997d-10bb-451e-9db3-8a000327815e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6796, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006796__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6797-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6797, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6798, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7119", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6798", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3981e21-5387-49b1-ab49-5e64e758aed6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6797, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006797__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6798-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6798, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6799, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7120", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6799", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfe71bef-5a49-4ba7-9b28-0f34ce604573", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 589955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6798, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006798__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6799-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6799, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6800, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7121", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6800", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ccf9c69-ddce-492a-9552-47e0f8fe23a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6799, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006799__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6800-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6800, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "589906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6801, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7122", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6801", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a42e3c09-b772-4151-b375-aa503bdc9031", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6800, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006800__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6801-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6801, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6802, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7123", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6802", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "463febec-448f-4f56-b173-4ef4d5c3a30d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6801, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006801__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6802-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6802, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6803, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7124", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6803", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "693898ca-5ec7-4878-8047-c63095cd1cf8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6802, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006802__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6803-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6803, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6804, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7125", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6804", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7a03a6a-97f3-40b0-863e-7c33148ab320", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6803, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006803__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6804-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6804, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6805, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7126", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6805", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82ec3dd2-ef0a-4786-97f7-b55e4d6d502a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6804, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006804__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6805-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6805, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6806, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7127", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6806", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "baf6f029-6abb-4b2a-8d3f-cbff048e6214", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6805, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006805__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6806-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6806, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6807, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7128", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6807", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e3d34a9-2211-4d3d-8ee4-46b182bf280d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6806, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006806__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6807-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6807, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6808, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7129", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6808", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cf67944-ca85-49ab-93d6-399e08815ec6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6807, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006807__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6808-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6808, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6809, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7130", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6809", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8d0aed0-8331-417a-98e4-5f0c2e91be15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6808, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006808__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6809-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6809, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6810, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7131", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6810", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da934f2a-3b3e-46f4-b38f-56d937e5541b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 590908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6809, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006809__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6810-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6810, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6811, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7132", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6811", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d97556e-c183-4bed-b392-65cbfe223b42", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6810, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006810__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6811-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6811, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6812, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7133", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6812", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50ed87bb-c98b-4ff7-8530-48c831d8b1a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6811, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006811__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6812-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6812, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "590953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6813, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7134", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6813", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "741729f8-d94c-493f-a5e4-fc2fd5e7e2b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6812, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006812__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6813-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6813, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6814, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7135", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6814", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e0c88a6-890a-4c48-a323-ff0587f96bc7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6813, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006813__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6814-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6814, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6815, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7136", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6815", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe4dfae0-681f-41f9-b016-b0b3808883d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6814, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006814__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6815-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6815, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6816, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7137", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6816", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1b0cee1-6965-4941-b05d-2003402f66c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6815, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006815__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6816-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6816, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6817, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7138", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6817", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0319d86e-a747-4320-bd6d-f0dca2da0514", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6816, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006816__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6817-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6817, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6818, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7139", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6818", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a774d4f9-1bea-4de2-9dcf-225bc74a5ec6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6817, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006817__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6818-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6818, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6819, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7140", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6819", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10050df7-5e9b-41b3-97cf-64e9a3b4320b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6818, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006818__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6819-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6819, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6820, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7141", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6820", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13c01d77-c4e0-42f7-9bb6-35252ddfd853", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6819, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006819__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6820-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6820, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6821, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7142", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6821", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f396af8-4195-4131-a631-eec016429b96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6820, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006820__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6821-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6821, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6822, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7143", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6822", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11bee223-5d18-4ab6-9349-afd490516e4c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 591955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6821, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006821__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6822-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6822, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6823, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7144", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6823", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99869a2a-8a08-4209-a467-36b5ed976313", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6822, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006822__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6823-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6823, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6824, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7145", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6824", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b49aa9d8-58da-4e4d-a3be-672629d415b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6823, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006823__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6824-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6824, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "591984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6825, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7146", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6825", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc6c2e5b-d07e-436f-b9bb-cd0f97dd25fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6824, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006824__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6825-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6825, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6826, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7147", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6826", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81e6047a-fb1d-405b-82db-9bcd3ec1b170", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6825, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006825__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6826-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6826, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6827, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7148", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6827", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0302beda-ba9b-4c62-acc8-954945c4dde8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6826, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006826__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6827-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6827, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6828, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7149", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6828", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "589618ca-7356-45d4-999d-ab47f9e3e01d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6827, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006827__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6828-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6828, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6829, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7150", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6829", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab0d70ab-9062-4d80-993a-5407f31865a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6828, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006828__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6829-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6829, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6830, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7151", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6830", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "276b59e4-63f4-46b9-ab6a-f832c4369a74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6829, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006829__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6830-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6830, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6831, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7152", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6831", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63c79950-a535-4993-8b6d-e2a1829338b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6830, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006830__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6831-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6831, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6832, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7153", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6832", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "946e4212-4c99-41d4-bb91-95463cc2f16f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6831, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006831__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6832-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6832, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6833, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7154", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6833", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8bde51fb-bdee-4c5b-a6d1-60bdc8a8ac8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6832, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006832__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6833-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6833, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6834, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7155", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6834", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "93edcd13-edea-4990-85c9-9ada32e6ea6f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 592986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6833, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006833__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6834-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6834, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6835, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7156", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6835", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "703c2ac9-fe8c-4a77-9e5e-206658369a5c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6834, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006834__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6835-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6835, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "592937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6836, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7157", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6836", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27af159f-14e3-4dfe-95a0-dd2e55dfce42", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6835, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006835__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6836-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6836, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6837, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7158", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6837", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cf42a21-8489-4e38-b1e7-93984e911d14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6836, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006836__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6837-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6837, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6838, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7159", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6838", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c41c26fc-34f4-4554-a94b-e3bae73c5cea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6837, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006837__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6838-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6838, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6839, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7160", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6839", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b0d5947-ceed-40a9-b58b-87652a14852f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6838, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006838__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6839-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6839, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6840, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7161", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6840", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54a197d3-ea9f-4292-9da2-73f8543faf0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6839, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006839__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6840-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6840, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6841, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7162", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6841", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d25cb12-1df4-4dea-8e77-ec6b132e4a92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6840, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006840__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6841-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6841, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6842, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7163", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6842", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a619757-df17-4116-975c-24c4dd15d435", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6841, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006841__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6842-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6842, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6843, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7164", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6843", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "177fb9a0-e3f2-4655-a91b-60eea221e90e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6842, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006842__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6843-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6843, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6844, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7165", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6844", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba8af5e0-bcb0-48cd-904e-c80488300454", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6843, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006843__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6844-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6844, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6845, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7166", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6845", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fbc68840-4f91-4a2b-af18-2fca250d7e13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 593940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6844, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006844__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6845-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6845, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6846, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7167", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6846", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "609845b5-7213-40d1-b00d-b5a7aa61d643", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6845, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006845__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6846-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6846, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6847, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7168", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6847", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e9e1736e-eb16-4990-8a21-bdbef79e8260", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6846, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006846__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6847-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6847, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "593984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6848, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7169", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6848", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a99a4656-c79e-4f38-b932-bce246e4d3c8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6847, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006847__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6848-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6848, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6849, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7170", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6849", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34b99756-55af-4be9-909e-6c46e8d09a18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6848, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006848__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6849-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6849, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6850, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7171", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6850", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f196178d-5423-4a22-8d18-b2edb552444d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6849, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006849__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6850-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6850, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6851, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7172", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6851", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9b18649f-82d7-4000-810a-0eff85be58e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6850, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006850__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6851-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6851, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6852, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7173", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6852", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fc3047b-b471-47fe-94b0-8fb5517a72df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6851, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006851__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6852-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6852, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6853, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7174", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6853", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82aa0cdb-9a5f-4cec-b4cb-b8207dba83fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6852, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006852__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6853-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6853, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6854, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7175", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6854", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2532cd73-0a8e-478f-9717-af0d752b958e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6853, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006853__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6854-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6854, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6855, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7176", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6855", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea402813-5be7-427d-9c1e-8746f23ae184", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6854, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006854__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6855-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6855, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6856, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7177", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6856", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4b2c6b3-ce8d-4b20-9426-6225bd17500b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6855, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006855__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6856-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6856, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6857, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7178", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6857", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e41a3719-8d27-40f7-aa7c-2b8422bb9d93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 594986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6856, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006856__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6857-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6857, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6858, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7179", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6858", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "69b45fee-6ca3-4aad-b8f0-7c51fe76357c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6857, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006857__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6858-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6858, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "594937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6859, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7180", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6859", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd6445e0-05dd-4b93-bfdd-45c874720543", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6858, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006858__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6859-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6859, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6860, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7181", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6860", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2a6c16d-f65d-4ea9-90c1-60e326369ae8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6859, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006859__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6860-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6860, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6861, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7182", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6861", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce3ef5fc-a873-47fd-839a-d6a9f05f5436", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6860, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006860__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6861-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6861, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6862, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7183", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6862", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "552bee49-d81d-4776-ad38-c512eb1d46d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6861, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006861__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6862-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6862, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6863, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7184", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6863", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2300277a-8045-483b-b755-896b42458c9d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6862, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006862__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6863-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6863, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6864, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7185", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6864", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e1fedcb-f848-4680-a9c1-d591a8f53932", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6863, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006863__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6864-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6864, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6865, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7186", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6865", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37cb0cac-fa09-4c9b-a46c-e7d666c056f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6864, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006864__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6865-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6865, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6866, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7187", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6866", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96483ad5-b107-4b92-a7ab-da20b2221b73", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6865, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006865__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6866-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6866, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6867, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7188", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6867", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0fc293c-1eca-4284-8d14-3de9b31511ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6866, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006866__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6867-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6867, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6868, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7189", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6868", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6334ab50-271e-4b53-86c2-84de5a3c09f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 595940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6867, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006867__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6868-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6868, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6869, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7190", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6869", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4be4129a-e3fd-4fd7-8c82-fd4ae3dfe8e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6868, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006868__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6869-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6869, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6870, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7191", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6870", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ff6e5e25-d6d5-402f-b2d4-23ac0b2abf9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6869, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006869__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6870-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6870, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "595984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6871, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7192", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6871", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8437269-dd89-4029-855c-3ee6197e8cb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6870, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006870__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6871-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6871, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6872, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7193", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6872", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ef7bad5-7442-4233-8180-1bf922f28413", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6871, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006871__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6872-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6872, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6873, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7194", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6873", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be76d051-7e8c-4ff5-83a2-14b0e7171e31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6872, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006872__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6873-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6873, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6874, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7195", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6874", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "545e9253-5a28-4ae7-b913-9e06b779e529", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6873, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006873__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6874-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6874, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6875, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7196", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6875", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8db7da87-b1f3-4a29-aa1f-d12108ae82ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6874, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006874__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6875-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6875, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6876, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7197", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6876", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f9a411f7-dea5-4c17-bf6f-40c9a89e19f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6875, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006875__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6876-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6876, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6877, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7198", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6877", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ccd89942-3e0b-4ceb-a9a1-df45ae3a780d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6876, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006876__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6877-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6877, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6878, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7199", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6878", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65b88015-0ee6-4885-83d3-3131f62d72de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6877, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006877__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6878-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6878, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6879, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7200", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6879", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc45cdae-fb87-443d-bc32-e9b748dc9332", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6878, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006878__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6879-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6879, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6880, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7201", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6880", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "090925d6-f249-4d5d-96f0-873ae1f15906", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 596986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6879, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006879__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6880-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6880, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6881, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7202", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6881", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06c2e5eb-1763-4583-8ffb-722327917e90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6880, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006880__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6881-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6881, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "596937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6882, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7203", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6882", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7179979e-593a-4f29-95c0-006ca19ab5e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6881, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006881__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6882-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6882, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6883, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:42 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7204", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6883", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "293d98b4-acee-40cc-97ac-7a573aab0481", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6882, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006882__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6883-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6883, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6884, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7205", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6884", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b7339eef-a8da-4564-a0ee-9ea261ecbaab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6883, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006883__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6884-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6884, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6885, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7206", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6885", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8f3295a-8cd9-4b8a-a42f-92f549fb5d44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6884, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006884__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6885-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6885, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6886, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7207", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6886", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "259e495c-8fb4-4850-ab42-9ce4bc2a4a8c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6885, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006885__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6886-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6886, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6887, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7208", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6887", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "230533ce-7e07-4ff0-b24b-19bb24ca071d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6886, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006886__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6887-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6887, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6888, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7209", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6888", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40d2c2d0-85c1-4644-8a55-7b27539ab5a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6887, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006887__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6888-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6888, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6889, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7210", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6889", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc9db1d8-6c78-40ff-9911-e258e8967a95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6888, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006888__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6889-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6889, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6890, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7211", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6890", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90bf6b69-a22c-43d5-a0ff-2a9aaadb1552", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6889, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006889__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6890-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6890, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6891, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7212", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6891", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "35cc10ea-b4a1-43b7-8b17-d66b28a7f185", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 597940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6890, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006890__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6891-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6891, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6892, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7213", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6892", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1243459-0a69-4000-8a98-9e12e6894ee8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6891, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006891__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6892-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6892, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6893, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7214", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6893", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c8fd56d-244a-48df-9d79-9047569bf85c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6892, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006892__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6893-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6893, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "597968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6894, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7215", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6894", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f37a2c99-bb91-4ec4-a74b-3742f29b3f47", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6893, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006893__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6894-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6894, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6895, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:43 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7216", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6895", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb24dd83-898b-4791-8a38-9d99c8ee3382", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6894, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006894__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6895-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6895, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6896, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7217", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6896", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97a2139f-3f65-445c-aaea-47fdf79ad01e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6895, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006895__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6896-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6896, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6897, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7218", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6897", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5cac9838-cd22-4898-8fa7-dbe38ad1c6b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6896, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006896__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6897-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6897, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6898, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7219", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6898", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14f72547-6038-49a6-a79c-6227ba5ec6c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6897, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006897__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6898-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6898, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6899, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7220", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6899", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3344b6d6-0c7e-4c5c-9c16-61e2337359b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6898, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006898__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6899-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6899, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6900, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7221", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6900", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "927c2d44-2977-4956-9bec-3daf84acd9c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6899, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006899__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6900-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6900, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6901, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7222", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6901", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "702938b4-5e60-41f7-b0a7-7d11ee345304", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6900, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006900__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6901-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6901, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6902, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7223", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6902", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "635da23c-a1d0-4b2a-a86c-b33235a326b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6901, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006901__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6902-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6902, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6903, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7224", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6903", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e50f99a-a2cf-4427-8929-5d61713792fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 598971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6902, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006902__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6903-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6903, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6904, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7225", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6904", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8adae8e4-d1db-44c7-8ebc-00649eb115e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6903, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006903__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6904-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6904, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "598921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6905, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7226", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6905", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f7bdbcb-b310-490d-a1b8-91b50979751f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6904, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006904__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6905-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6905, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6906, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:44 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7227", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6906", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f21a4977-ca04-4b24-a453-66a78a10018a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6905, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006905__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6906-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6906, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6907, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7228", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6907", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7c8c360-6f58-4689-9a2f-d00404d8fca7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6906, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006906__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6907-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6907, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6908, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7229", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6908", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dafb485f-7ff0-4741-9652-329721c63b0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6907, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006907__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6908-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6908, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6909, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7230", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6909", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e3732fb-5346-4557-a289-abf3e4078703", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6908, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006908__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6909-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6909, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6910, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7231", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6910", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb5471b3-6278-4fac-b485-4a3e9becc73f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6909, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006909__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6910-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6910, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6911, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7232", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6911", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef760f7a-1db0-43a0-8c7d-53cd8da7c007", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6910, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006910__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6911-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6911, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6912, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7233", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6912", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83addb9a-cd6d-4c44-89ae-11ec43c3ee17", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6911, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006911__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6912-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6912, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6913, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7234", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6913", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef5c18ab-d21e-4a36-90e7-6713c7c6b60b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6912, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006912__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6913-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6913, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6914, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7235", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6914", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d82509ec-4a23-4045-98dc-9233be4e6902", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6913, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006913__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6914-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6914, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6915, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7236", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6915", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e751db5-4bef-4d21-82d6-d4d940a0623f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6914, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006914__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6915-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6915, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6916, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7237", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6916", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a285506-cbe4-4d84-9b78-942de9f9b2db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6915, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006915__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6916-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6916, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "599968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6917, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7238", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6917", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd22eed1-2cee-4a6f-8040-2e07193e54ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6916, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006916__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6917-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6917, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6918, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:45 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7239", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6918", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f83221f8-65d7-445e-8c38-3638fa7d100d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6917, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006917__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6918-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6918, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6919, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7240", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6919", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "836aac1a-5ed4-442f-b197-080d17a2fbf7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6918, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006918__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6919-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6919, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6920, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7241", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6920", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2141d9b7-7163-47bb-9316-f991c7d25b77", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6919, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006919__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6920-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6920, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6921, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7242", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6921", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34f8ce82-f378-4619-8a68-bcd16ebfd0c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6920, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006920__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6921-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6921, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6922, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7243", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6922", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "571de055-f731-41c8-a483-84f046d931c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6921, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006921__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6922-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6922, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6923, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7244", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6923", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "016b86e1-72b9-48d4-af66-b8b9476a8e13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6922, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006922__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6923-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6923, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6924, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7245", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6924", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a2ff6bd-1cc7-48ea-b445-f56246feb49c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6923, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006923__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6924-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6924, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6925, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7246", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6925", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29aef8ec-93cd-4526-8274-afb34112b9f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6924, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006924__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6925-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6925, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6926, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7247", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6926", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abbdd035-accb-4ac6-ab0a-11b284ba18a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 600971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6925, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006925__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6926-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6926, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6927, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7248", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6927", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8e6ab653-9095-432e-b1cb-158d0c1cd9be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6926, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006926__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6927-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6927, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "600921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6928, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7249", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6928", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c84efac7-730f-465c-a6b2-aa647865a7dd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6927, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006927__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6928-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6928, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6929, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7250", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6929", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5f07f2b-1eeb-42c8-b5d4-b0a870527b8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6928, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006928__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6929-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6929, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6930, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7251", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6930", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "583e4f34-c6c1-4642-bebc-9e89bc846e46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6929, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006929__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6930-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6930, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6931, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7252", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6931", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00b9c201-56f8-42ee-9fb8-1b7bec729d18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6930, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006930__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6931-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6931, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6932, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7253", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6932", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cf37e3b-cdbd-4cc6-adbf-dba706e53704", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6931, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006931__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6932-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6932, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6933, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7254", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6933", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3b0d3dc-91e4-4f54-9d4b-52048bcd0cf1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6932, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006932__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6933-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6933, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6934, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7255", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6934", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55bac866-41d3-4608-98d4-6888972b2c99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6933, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006933__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6934-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6934, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6935, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7256", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6935", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c67f5ef-1a82-4aec-85e5-9d0023187fed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6934, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006934__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6935-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6935, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6936, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7257", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6936", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bd3e800a-6ce6-426c-ba21-9a0a885d17f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6935, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006935__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6936-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6936, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6937, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7258", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6937", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ddfb7f3-e53c-4a62-a30b-6c29840c8ea3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 601924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6936, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006936__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6937-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6937, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6938, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7259", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6938", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3eef35f9-b0c0-4c9b-96c9-0377f58abf3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6937, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006937__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6938-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6938, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6939, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7260", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6939", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40c09c7d-a532-4ef6-b8a6-507cc9204b79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6938, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006938__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6939-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6939, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "601968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6940, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7261", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6940", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30cbbae0-78e8-4d3e-9973-105e2b800d13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6939, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006939__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6940-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6940, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6941, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7262", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6941", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62c7ff98-6e55-49bb-aed5-d0cb04672c90", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6940, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006940__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6941-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6941, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6942, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7263", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6942", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25cb6f8b-d387-48f0-8e17-9f744ed15278", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6941, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006941__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6942-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6942, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6943, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7264", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6943", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b99e5fe5-1774-47d7-86dd-2ec984ad2b04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6942, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006942__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6943-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6943, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6944, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7265", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6944", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "315a4e9c-8e9e-4132-9bbc-bcd29a5bb5cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6943, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006943__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6944-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6944, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6945, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7266", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6945", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a8fbec4-77d2-414d-b9bf-c870d6d0d217", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6944, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006944__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6945-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6945, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6946, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7267", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6946", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c8ccc55-8519-48a0-b4b1-2ad7f62d3163", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6945, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006945__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6946-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6946, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6947, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7268", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6947", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "430b3d7a-1b6b-4ee8-8bb0-bef17bf6aa14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6946, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006946__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6947-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6947, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6948, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7269", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6948", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30cc7797-16e2-466f-9470-a2241896414e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6947, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006947__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6948-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6948, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6949, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7270", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6949", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6ee9f1f-b208-4037-9b73-efdcffa8d9c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 602971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6948, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006948__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6949-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6949, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6950, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7271", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6950", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c84cbc63-cbfe-4125-8fed-bc49912deefb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6949, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006949__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6950-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6950, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "602921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6951, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7272", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6951", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "871beb9e-9fe2-4d04-8a15-f926bd9b778f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6950, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006950__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6951-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6951, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6952, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7273", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6952", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "154539c0-050d-4a65-ac6b-332ed0b27841", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6951, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006951__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6952-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6952, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6953, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7274", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6953", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "982eaa14-fb93-479a-94d1-311793f97f5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6952, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006952__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6953-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6953, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6954, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7275", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6954", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ca84b72-3ba8-4936-b3e0-a8833e3013d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6953, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006953__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6954-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6954, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6955, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7276", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6955", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71e71bf5-1067-4775-a1c8-b3656c6cfeb4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6954, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006954__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6955-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6955, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6956, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7277", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6956", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0262a69-6b00-40dc-8a55-de817f430d46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6955, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006955__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6956-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6956, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6957, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7278", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6957", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f1e6b81-0055-429c-91e4-8b3344b402ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6956, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006956__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6957-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6957, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6958, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7279", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6958", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a62dbe7c-1412-4245-8ed1-c3cf0a5e545d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6957, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006957__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6958-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6958, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6959, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7280", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6959", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa7e2845-13a2-4844-865d-98c7a54c6216", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6958, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006958__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6959-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6959, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6960, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7281", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6960", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ba9ec99-5126-429d-bb6d-ba39d1d15edc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 603924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6959, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006959__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6960-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6960, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6961, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7282", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6961", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b777f54-8f26-4854-954c-9c6f3907e8dc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6960, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006960__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6961-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6961, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6962, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7283", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6962", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adf812be-e6b8-4fc8-bf60-8e0ca4da26e3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6961, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006961__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6962-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6962, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "603953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6963, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7284", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6963", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "847e6b7e-9491-40f2-b3c6-b84633a3a105", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6962, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006962__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6963-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6963, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6964, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7285", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6964", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ba71f3e6-763a-45f5-a6bf-1ecd81103608", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6963, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006963__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6964-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6964, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6965, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7286", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6965", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af293890-fbe9-4d6d-ad95-213d93ca6ab8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6964, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006964__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6965-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6965, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6966, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7287", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6966", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90dcaf1c-a480-41ef-a996-245b8fb55f12", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6965, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006965__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6966-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6966, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6967, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7288", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6967", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c1571fe-f120-4d0f-a6eb-fe49ee1447a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6966, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006966__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6967-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6967, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6968, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7289", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6968", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "957c7e34-e89a-488b-94bf-eef0befa132d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6967, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006967__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6968-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6968, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6969, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7290", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6969", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ce7511e-7b93-4b12-ab71-128727e4568d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6968, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006968__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6969-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6969, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6970, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7291", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6970", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "110dc089-59dd-4cd7-81b7-c89d0988b3bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6969, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006969__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6970-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6970, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6971, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7292", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6971", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd37f080-95f4-4fe7-89ee-575c8edcb06e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6970, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006970__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6971-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6971, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6972, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7293", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6972", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f60b4e4-5b46-4f2c-8da5-1640e6edf4f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 604955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6971, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006971__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6972-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6972, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6973, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7294", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6973", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1dab983-5786-4974-974b-6f21e2a4bcf3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6972, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006972__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6973-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6973, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "604906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6974, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7295", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6974", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4f04f22-24f5-42ef-86d6-55fa30eca83a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6973, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006973__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6974-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6974, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6975, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7296", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6975", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58f42bef-ab75-4aa3-8368-35c954c4410e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6974, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006974__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6975-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6975, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6976, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7297", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6976", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "113dba9a-3ea3-4fab-a88c-c56088019ef6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6975, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006975__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6976-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6976, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6977, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7298", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6977", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1c43368-131b-4367-9ab3-2187802d3da1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6976, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006976__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6977-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6977, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6978, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7299", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6978", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46959ae9-b231-409b-9344-1302120b732c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6977, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006977__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6978-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6978, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6979, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7300", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6979", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e48514eb-b9b4-4da1-990d-ac80d14a3ef0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6978, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006978__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6979-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6979, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6980, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7301", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6980", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36e8bd54-98de-4a30-9dff-2d8b5e269352", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6979, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006979__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6980-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6980, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6981, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7302", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6981", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "760ee8af-647a-4169-b746-b7afe9cd3326", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6980, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006980__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6981-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6981, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6982, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7303", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6982", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98093950-718c-4653-b7d7-17987ac50f85", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6981, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006981__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6982-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6982, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6983, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7304", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6983", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4f24204-af82-4088-aa1c-7ca565a81da4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 605908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6982, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006982__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6983-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6983, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6984, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7305", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6984", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "52d74aa4-b7fa-4dda-b7cf-12b54f17c605", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6983, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006983__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6984-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6984, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6985, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7306", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6985", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ced79a38-ddb4-4fce-96ba-194992dcf19c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6984, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006984__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6985-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6985, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "605953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6986, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7307", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6986", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a851eeb-c008-494e-b674-65879739bd87", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6985, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006985__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6986-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6986, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6987, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7308", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6987", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40deeedb-8888-4387-93cd-1641a4adc7eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6986, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006986__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6987-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6987, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6988, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7309", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6988", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "389132c1-2616-4b1b-b70e-f4135b95a4f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6987, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006987__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6988-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6988, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6989, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7310", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6989", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97ee0077-e357-45e7-956d-ea65f98ce1a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6988, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006988__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6989-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6989, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6990, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7311", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6990", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57ae17d1-ae58-4465-8327-e0bd2939e911", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6989, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006989__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6990-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6990, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6991, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7312", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6991", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4529b660-d962-42b4-a1aa-eefd67fcafad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6990, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006990__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6991-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6991, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6992, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7313", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6992", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20bd74e4-21e6-41d8-a282-171605ace5ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6991, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006991__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6992-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6992, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6993, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7314", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6993", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48373def-5e89-476b-b944-772c4089ac6c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6992, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006992__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6993-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6993, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6994, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7315", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6994", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76298077-3761-49ff-9dfe-5915a0e5e047", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6993, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006993__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6994-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6994, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6995, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7316", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6995", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1cb06a8e-c7b1-47c9-9f6c-e62dc12b7c0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 606955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6994, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006994__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6995-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6995, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6996, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7317", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6996", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b265a8c6-dd97-47e5-baa5-ab230b6b93e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6995, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006995__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6996-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6996, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "606906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6997, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7318", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6997", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea0fe563-afed-436e-87da-ab1c7f5dc710", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6996, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006996__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6997-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6997, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6998, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7319", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6998", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "737fef40-25f9-4eae-a991-6e0a1622e32c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6997, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006997__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6998-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6998, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 6999, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7320", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "6999", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f37e3437-2ccc-4be7-b5b9-695a84ccf9cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6998, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006998__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-6999-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 6999, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7000, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7321", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7000", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "25f585af-f71d-4ea9-b906-345fa26c6559", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 6999, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000006999__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7000-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7000, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7001, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7322", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7001", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5c1b44a-428d-4a27-8bc2-8edfe7f2fcee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7000, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7001-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7001, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7002, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7323", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7002", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94319f52-b0fb-4ce2-bff7-31fad180033b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7001, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007001__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7002-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7002, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7003, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7324", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7003", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "095170f0-c956-403f-a541-f20231703d1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7002, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007002__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7003-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7003, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7004, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7325", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7004", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3a8d5dd-c548-4a03-8817-688bb80c5bb9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7003, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007003__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7004-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7004, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7005, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7326", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7005", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6b1d65c-af76-4a63-9598-d7b885f77d8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7004, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007004__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7005-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7005, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7006, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7327", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7006", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "14c89f06-30bb-4811-97ee-7c9734953cba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 607908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7005, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007005__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7006-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7006, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7007, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7328", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7007", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a48cf1a5-91cf-4288-b052-db63646d5546", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7006, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007006__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7007-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7007, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7008, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7329", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7008", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a19c950a-55c6-40ad-8a7f-21bb237a4eda", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7007, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007007__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7008-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7008, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "607953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7009, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7330", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7009", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59bce613-5bfb-44c5-9765-6219381942a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7008, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007008__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7009-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7009, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7010, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7331", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7010", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4aa79d41-a5b9-46a6-b7ed-ea0deb241696", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7009, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007009__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7010-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7010, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7011, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7332", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7011", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1025c17e-86ed-4b34-81f5-44cc2c9d09c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7010, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007010__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7011-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7011, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7012, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7333", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7012", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36d42f32-c26f-45ff-8fda-92d96904acd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7011, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007011__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7012-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7012, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7013, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7334", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7013", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ea0e3e2a-1fe0-45ed-9209-5a2dbf3b784d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7012, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007012__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7013-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7013, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7014, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7335", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7014", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6124a465-21be-4383-bcef-5d7c5d2962b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7013, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007013__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7014-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7014, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7015, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7336", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7015", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "77e7d4c9-49d1-4243-849b-d6af41c489f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7014, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007014__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7015-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7015, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7016, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7337", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7016", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "adf8de62-4e71-4972-ac29-459fabd65b97", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7015, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007015__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7016-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7016, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7017, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7338", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7017", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eeeebbee-06d7-4691-86c4-f173df581589", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7016, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007016__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7017-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7017, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7018, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7339", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7018", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43669b25-45a6-46ec-a741-7d34bdbbafff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 608955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7017, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007017__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7018-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7018, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7019, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7340", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7019", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2aaf6b18-3493-4cf9-b714-31c7e41bb387", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7018, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007018__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7019-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7019, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7020, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7341", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7020", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a52298d-24fa-487c-883a-39cb9527eaf8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7019, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007019__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7020-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7020, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "608984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7021, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7342", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7021", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c87d0c57-51e5-4e45-bfbd-9a92f6846c0a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7020, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007020__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7021-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7021, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7022, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7343", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7022", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7e2981a4-57f6-4842-9e0c-5da66637ffc0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7021, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007021__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7022-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7022, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7023, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7344", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7023", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c030242f-472f-4654-bed0-0860fce42f3e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7022, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007022__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7023-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7023, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7024, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7345", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7024", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8cc43594-24c1-495a-8103-33f9c521a4d4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7023, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007023__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7024-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7024, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7025, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7346", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7025", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdb35720-d10f-4a23-9db0-8f5d1a878b78", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7024, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007024__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7025-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7025, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7026, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7347", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7026", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a6695156-c2e7-4a54-8a61-f21a9f863d32", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7025, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007025__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7026-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7026, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7027, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7348", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7027", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23e455b4-7a49-412c-8236-f158f5b2a47a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7026, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007026__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7027-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7027, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7028, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7349", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7028", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "93e9ba26-d3b4-4c5f-8ed4-dea622a5a2aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7027, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007027__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7028-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7028, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7029, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7350", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7029", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4ef6851-cca2-421e-99ff-66b1ef6e1005", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7028, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007028__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7029-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7029, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7030, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7351", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7030", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4bc4077-ee3d-4a48-adc2-55672ab510b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 609986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7029, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007029__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7030-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7030, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7031, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7352", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7031", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f68e59f6-af4c-4cbf-b7f3-086c6e53522b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7030, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007030__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7031-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7031, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "609937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7032, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7353", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7032", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fad0bb89-7dae-4b0f-af49-7258ef3101a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7031, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007031__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7032-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7032, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7033, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7354", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7033", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbeb3e5c-b6fb-43d7-a74a-3a492749b43f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7032, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007032__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7033-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7033, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7034, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7355", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7034", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a80cb81a-fa2f-4d40-9155-fd19b75bc755", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7033, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007033__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7034-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7034, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7035, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7356", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7035", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa9ee6ac-57e5-421d-aa38-89bfcbd6a107", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7034, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007034__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7035-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7035, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7036, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7357", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7036", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2fa88e35-2225-4124-86b2-674f74d71f1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7035, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007035__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7036-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7036, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7037, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7358", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7037", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7198351a-efb2-4922-88b2-8ac0446ef194", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7036, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007036__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7037-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7037, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7038, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7359", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7038", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aed8c287-4380-45b9-95c9-33857fee90ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7037, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007037__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7038-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7038, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7039, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7360", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7039", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3e771e2e-2e8c-426f-a3dd-191d568f6c44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7038, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007038__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7039-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7039, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7040, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7361", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7040", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fc5c0004-c987-4ae1-b3cd-a4dfea6aa332", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7039, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007039__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7040-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7040, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7041, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7362", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7041", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98978008-5404-42e5-bcf3-beb7b8e4e024", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 610940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7040, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007040__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7041-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7041, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7042, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7363", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7042", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fa1ea3d-a114-4c66-9519-560f262944fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7041, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007041__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7042-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7042, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7043, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7364", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7043", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bbc7cbd8-5c2a-4611-b16f-32b5de881b75", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7042, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007042__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7043-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7043, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "610984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7044, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7365", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7044", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6e30fcb-2631-4eab-abf6-8f8f2e143f21", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7043, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007043__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7044-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7044, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7045, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7366", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7045", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f0bd671-b455-4537-a695-68757d7f3a1a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7044, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007044__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7045-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7045, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7046, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7367", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7046", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08a581a2-981a-4705-88c1-cf8b60592996", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7045, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007045__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7046-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7046, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7047, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7368", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7047", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "827daf5b-bbc1-4bc9-9bde-b85f10cd5e81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7046, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007046__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7047-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7047, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7048, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7369", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7048", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e88d1a5b-27c5-4269-9056-a3c89195038d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7047, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007047__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7048-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7048, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7049, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7370", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7049", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "312fe3fa-4b4d-4d09-8628-1b915799cd59", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7048, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007048__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7049-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7049, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7050, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7371", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7050", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "461b34d3-d9db-47aa-a16b-8b244d5e1bba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7049, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007049__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7050-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7050, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7051, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7372", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7051", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "efe489d6-bad8-4777-bbd9-3342127614ba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7050, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007050__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7051-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7051, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7052, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7373", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7052", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b92b2edd-d46f-453f-808f-c5a0af8b6cb6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7051, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007051__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7052-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7052, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7053, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7374", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7053", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e87cf81c-2da2-47cb-99f0-5180fd7d7fe3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 611986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7052, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007052__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7053-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7053, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7054, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7375", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7054", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f80e118e-8a9a-471d-847a-43e6a6ad4572", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7053, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007053__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7054-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7054, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "611937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7055, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7376", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7055", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f8ce600-598d-4027-a1b3-acd621fe6561", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7054, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007054__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7055-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7055, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7056, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7377", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7056", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27d59ecf-743f-495b-b723-3e5e1bd1cad9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7055, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007055__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7056-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7056, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7057, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7378", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7057", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fedfd39c-6316-4943-a37e-c824295be530", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7056, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007056__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7057-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7057, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7058, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7379", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7058", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "910636f6-82ae-4416-a141-4d57871046df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7057, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007057__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7058-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7058, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7059, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7380", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7059", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d40c9ce-b6d6-4814-9a42-76c2d0944f48", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7058, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007058__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7059-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7059, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7060, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7381", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7060", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a121a981-599b-48b0-bc2a-c24d92cbf97f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7059, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007059__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7060-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7060, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7061, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7382", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7061", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87fb350c-bd5e-4468-a58b-6468480e0195", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7060, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007060__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7061-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7061, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7062, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7383", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7062", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21222ed0-2054-4f2d-ba92-43245289e079", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7061, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007061__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7062-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7062, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7063, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7384", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7063", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d3e0f01-ee3a-413e-b360-3fda9d61dd2f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7062, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007062__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7063-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7063, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7064, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7385", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7064", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2783b16a-d2ec-4edd-9d35-69253a359729", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 612940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7063, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007063__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7064-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7064, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7065, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7386", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7065", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9142e4e6-b1e8-43c0-bfd9-e03e01badb2a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7064, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007064__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7065-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7065, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7066, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7387", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7066", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "21091738-24bf-463b-b5bb-226b37aab194", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7065, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007065__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7066-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7066, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "612984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7067, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7388", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7067", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d79be99-064a-4668-b049-74f5e57e4ee3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7066, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007066__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7067-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7067, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7068, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7389", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7068", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2b46ed3-813e-4495-8feb-a7b969f9a0a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7067, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007067__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7068-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7068, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7069, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7390", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7069", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81c635c9-ef78-4db2-82b4-984662436c5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7068, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007068__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7069-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7069, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7070, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7391", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7070", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76abe51d-c9b4-4325-875c-11fc122dde64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7069, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007069__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7070-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7070, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7071, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7392", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7071", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec73271d-581d-4fad-b6b7-50195a22fe1e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7070, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007070__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7071-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7071, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7072, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7393", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7072", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "513df88b-6e05-4b17-ae4c-de43a08b456b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7071, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007071__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7072-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7072, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7073, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7394", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7073", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9976566-841f-4343-abda-9be8fe36bda9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7072, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007072__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7073-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7073, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7074, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7395", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7074", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0b6d6192-ecb0-4fbc-bf71-f7e875f91d36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7073, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007073__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7074-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7074, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7075, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7396", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7075", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51f7e0a1-00e8-4e54-9bb7-167cee2bd349", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7074, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007074__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7075-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7075, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7076, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7397", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7076", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef8b31b2-91c2-4517-a059-413760d2cd7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 613986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7075, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007075__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7076-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7076, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7077, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7398", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7077", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c4c897b-b12b-4eb5-9612-d8c86d114165", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7076, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007076__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7077-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7077, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "613937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7078, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7399", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7078", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2e63d82d-d3c2-43ba-a38d-9751ed2008eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7077, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007077__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7078-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7078, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7079, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:28:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7400", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7079", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "152b439a-c14e-4768-902d-6793c75ee486", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7078, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007078__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7079-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7079, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7080, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7401", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7080", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3974c4a3-d501-4143-9f76-1ffac3e64f4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7079, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007079__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7080-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7080, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7081, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7402", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7081", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc551806-0fbb-492d-b369-2d4ec1dae534", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7080, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007080__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7081-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7081, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7082, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7403", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7082", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f616cce3-d106-402c-94fe-b49782391446", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7081, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007081__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7082-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7082, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7083, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7404", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7083", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed21286e-cab7-43b3-a6c4-9260cf733239", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7082, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007082__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7083-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7083, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7084, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7405", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7084", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "82810ff0-204a-40cc-9479-b5352d5e2a7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7083, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007083__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7084-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7084, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7085, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7406", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7085", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "367d51f2-af26-4952-8635-e358ac7a68f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7084, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007084__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7085-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7085, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7086, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7407", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7086", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bde23fe-fada-46ce-96f2-6fa41b4c93f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7085, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007085__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7086-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7086, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7087, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7408", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7087", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fa9758a4-593a-41ff-979f-c6d9145a53de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 614940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7086, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007086__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7087-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7087, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7088, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7409", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7088", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64004634-56a5-49e2-bc3e-93b6638e7791", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7087, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007087__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7088-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7088, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7089, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7410", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7089", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "969dc38c-97fc-4704-a5f5-791a79f1b605", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7088, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007088__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7089-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7089, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "614968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7090, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7411", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7090", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24bb23e1-7462-4ebc-ac76-b78c048f5fd5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7089, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007089__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7090-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7090, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7091, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7412", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7091", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c3a3638-0a05-4ae1-8a5c-81131ae27f10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7090, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007090__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7091-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7091, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7092, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7413", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7092", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4d10a68-21be-4322-abaf-1d50e54ecc44", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7091, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007091__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7092-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7092, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7093, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7414", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7093", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "621acdf5-dcf4-4c5d-92da-e0092db548bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7092, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007092__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7093-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7093, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7094, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7415", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7094", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c19f6586-d911-4531-9475-6ff9becf6e00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7093, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007093__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7094-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7094, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7095, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7416", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7095", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d3d61bc9-98bd-41ea-9bbc-2f6868873f8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7094, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007094__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7095-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7095, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7096, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7417", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7096", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6557b234-a76b-4f82-9428-10fb15d44dab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7095, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007095__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7096-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7096, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7097, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7418", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7097", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90495042-f769-4821-bf7d-f6a4f7c663e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7096, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007096__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7097-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7097, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7098, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7419", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7098", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c021a8d-0b67-4668-85b0-b738df289bf4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7097, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007097__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7098-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7098, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7099, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7420", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7099", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f183569-db83-4863-be62-c73bb60ba04a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 615971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7098, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007098__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7099-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7099, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7100, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7421", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7100", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64bc4bd0-c6a2-4d83-92f2-04d6d7290435", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7099, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007099__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7100-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7100, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "615921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7101, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7422", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7101", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24ae5243-d304-4aba-9fbe-9681a079df50", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7100, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007100__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7101-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7101, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7102, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7423", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7102", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7858e677-6063-4691-8f75-1dfe42aba607", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7101, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007101__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7102-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7102, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7103, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7424", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7103", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5faf2c77-f0ae-47ca-9c20-78512a7e75f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7102, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007102__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7103-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7103, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7104, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7425", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7104", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b266c573-2158-4ea3-ad52-0dfda6a291ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7103, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007103__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7104-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7104, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7105, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7426", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7105", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0dc57c43-033c-4a4f-9f27-61775eb51cfb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7104, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007104__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7105-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7105, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7106, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7427", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7106", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63c4190a-5e7d-4199-9626-0b5ea354849c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7105, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007105__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7106-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7106, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7107, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7428", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7107", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ed06636-c26b-4e67-bdb6-bf4e3221621a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7106, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007106__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7107-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7107, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7108, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7429", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7108", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fe86aed-1a3b-4bfe-8735-a3522a4f8ecf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7107, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007107__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7108-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7108, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7109, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7430", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7109", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fce49d2d-c709-49f7-abf9-48448fa8411e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7108, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007108__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7109-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7109, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7110, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7431", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7110", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11274351-7323-41e8-b90e-f67dad85b17e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 616924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7109, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007109__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7110-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7110, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7111, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7432", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7111", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b337467-333c-443c-92e6-139898844006", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7110, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007110__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7111-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7111, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7112, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7433", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7112", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed89a088-3169-409f-82d3-6df04983282e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7111, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007111__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7112-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7112, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "616968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7113, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7434", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7113", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d8df4b5-097a-4c7b-b3ee-6c3db4365217", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7112, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007112__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7113-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7113, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7114, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7435", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7114", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aac6c543-f6b4-4999-ab18-9d51b3e4b87b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7113, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007113__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7114-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7114, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7115, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7436", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7115", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c68e2e7-f1e2-4bb8-b511-e0a6fc60ffea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7114, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007114__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7115-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7115, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7116, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7437", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7116", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b474c5f1-df79-479b-baa4-121b8213fc7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7115, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007115__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7116-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7116, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7117, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7438", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7117", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a6d98a0-9fbb-4f1a-ac52-d26ce629398f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7116, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007116__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7117-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7117, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7118, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7439", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7118", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbf1aa72-6c10-46d4-8c59-256588b9cb29", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7117, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007117__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7118-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7118, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7119, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7440", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7119", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6311ae11-5393-4df5-8ce0-a50c50692cde", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7118, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007118__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7119-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7119, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7120, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7441", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7120", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "321b86ce-8656-4f91-88b5-c3c7d61e2f23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7119, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007119__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7120-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7120, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7121, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7442", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7121", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7fea1564-bc0d-4a86-a062-e0a1fb929dd4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7120, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007120__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7121-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7121, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7122, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7443", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7122", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7dfe7d62-69b4-473b-a387-e77deccf0d14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 617971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7121, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007121__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7122-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7122, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7123, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7444", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7123", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f2f66245-c4e6-480b-8e3e-ed6651de450a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7122, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007122__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7123-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7123, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "617921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7124, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7445", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7124", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68f46496-8ee4-4dfd-90ca-530b188807ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7123, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007123__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7124-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7124, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7125, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7446", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7125", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "659d26c4-e58a-4270-aab2-a5f7f4bb6696", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7124, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007124__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7125-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7125, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7126, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7447", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7126", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0222b3a4-b870-4f2e-b7a4-55d2c022fe2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7125, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007125__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7126-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7126, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7127, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7448", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7127", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60ced852-c71b-4564-a75c-265bd5491140", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7126, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007126__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7127-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7127, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7128, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7449", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7128", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fcd596a5-4807-4a47-9332-ec5d94e840ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7127, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007127__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7128-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7128, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7129, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7450", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7129", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee64708d-2d04-4a26-bc25-ba5417a613d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7128, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007128__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7129-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7129, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7130, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7451", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7130", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "035cfdca-bbe8-4473-8ac5-3f87f0c91770", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7129, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007129__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7130-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7130, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7131, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7452", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7131", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "def5c8ef-4c63-4f2d-9a44-405c0afa7def", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7130, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007130__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7131-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7131, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7132, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7453", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7132", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c479d1dc-a2a6-42b1-83cd-a7c650b6cd2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7131, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007131__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7132-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7132, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7133, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7454", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7133", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39802e4e-3f87-4344-9bf9-3fdc2b8655b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 618924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7132, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007132__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7133-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7133, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7134, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7455", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7134", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ead2f5d-b9b8-447d-8b83-4733554e5d6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7133, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007133__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7134-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7134, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7135, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7456", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7135", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86659ebe-b0ef-4354-a00c-211cfa59b50d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7134, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007134__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7135-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7135, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "618968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7136, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7457", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7136", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60bf2e3f-848b-44d2-9cfb-df8f429cb0c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7135, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007135__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7136-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7136, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7137, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:04 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7458", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7137", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3362a1b5-0d07-444a-bceb-b7bda4e3c37a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7136, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007136__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7137-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7137, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7138, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7459", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7138", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fe987f5c-73b2-4890-93e4-95db870727ec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7137, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007137__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7138-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7138, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7139, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7460", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7139", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "850f9837-718f-43fd-b018-fded96408f0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7138, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007138__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7139-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7139, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7140, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7461", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7140", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1391c15f-aa61-425d-96b9-2ebe87b6efdd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7139, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007139__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7140-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7140, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7141, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7462", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7141", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de796b09-9f9a-4b5d-acc7-193c5b0bf134", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7140, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007140__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7141-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7141, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7142, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7463", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7142", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28f83602-d1e7-4a81-a277-4b97243a2df3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7141, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007141__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7142-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7142, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7143, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7464", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7143", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e01878de-b5e2-4b5d-a2df-27c92b328a96", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7142, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007142__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7143-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7143, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7144, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7465", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7144", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34f20099-f034-4774-a675-a542cb42f9d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7143, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007143__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7144-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7144, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7145, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7466", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7145", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5d960e3-0ddd-425d-b21a-21910d45de64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 619971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7144, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007144__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7145-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7145, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7146, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7467", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7146", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7a622b09-372a-4eed-bf9a-6dae232b9f05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7145, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007145__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7146-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7146, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "619921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7147, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7468", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7147", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8f3afb4-8830-4191-b5f1-dd37cfa59404", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7146, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007146__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7147-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7147, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7148, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7469", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7148", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5da6ec69-ae31-47ae-8d6e-835cfb492a01", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7147, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007147__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7148-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7148, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7149, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:05 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7470", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7149", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1449dc56-2dd3-4bc8-8e19-b07694b9eb6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7148, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007148__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7149-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7149, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7150, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7471", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7150", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d2163e4-3713-4124-ab0c-75f2937b56e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7149, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007149__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7150-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7150, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7151, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7472", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7151", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c9b16b23-41e0-4fc7-9401-9b2e15f44bed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7150, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007150__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7151-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7151, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7152, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7473", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7152", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6ed217d-bdfe-4d5a-b7fc-a8de7eb0e260", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7151, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007151__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7152-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7152, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7153, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7474", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7153", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "32e82f56-b8dd-472c-80c7-e4af8834ec5b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7152, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007152__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7153-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7153, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7154, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7475", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7154", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd51460c-9f2a-4d9a-b653-c2e09d670094", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7153, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007153__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7154-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7154, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7155, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7476", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7155", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "95fd14ca-971e-4e98-977d-f8b394a76ef9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7154, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007154__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7155-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7155, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7156, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7477", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7156", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d92e1ac-becd-42e2-89c5-1b00347730a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7155, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007155__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7156-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7156, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7157, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7478", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7157", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8988c1e-e693-4c80-a2eb-3723b874e08c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7156, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007156__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7157-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7157, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7158, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7479", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7158", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "994d0239-d503-4ca0-a23a-9fc186a77722", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7157, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007157__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7158-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7158, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "620953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7159, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7480", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7159", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bd77aba-2fe1-4bf9-ab01-c72db161bf6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7158, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007158__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7159-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7159, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7160, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:06 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7481", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7160", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7d521ff2-593a-4f7a-b5e9-df4c39e4861b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7159, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007159__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7160-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7160, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7161, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7482", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7161", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "542915eb-2dac-4242-895f-93b87699674c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7160, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007160__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7161-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7161, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7162, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7483", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7162", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd474b50-5a51-4bbd-97be-f67f45d345e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7161, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007161__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7162-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7162, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7163, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7484", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7163", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "12efe7d7-1a46-4352-9909-567cfa513a1b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7162, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007162__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7163-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7163, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7164, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7485", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7164", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51c3028c-a0b2-4b59-92e8-258f599c5752", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7163, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007163__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7164-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7164, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7165, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7486", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7165", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b464882a-a3c6-4589-bc27-e8c7e326b5d9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7164, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007164__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7165-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7165, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7166, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7487", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7166", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "669dcf72-8ec1-4aa1-84a2-5fdbc0251491", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7165, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007165__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7166-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7166, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7167, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7488", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7167", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "85b8fe6d-6502-4cfb-af6e-6274c0294652", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7166, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007166__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7167-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7167, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7168, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7489", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7168", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a540ab3-6206-44b5-a34f-de5997a34b5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 621955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7167, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007167__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7168-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7168, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7169, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7490", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7169", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e34e185c-2fc7-4b4e-a978-2cf6e6d513e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7168, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007168__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7169-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7169, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "621906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7170, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7491", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7170", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1becb428-f5e6-4a23-83e6-dbbb28f78568", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7169, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007169__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7170-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7170, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7171, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7492", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7171", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d89011fb-8a27-4948-9251-e47ff5bb03d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7170, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007170__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7171-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7171, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7172, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:07 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7493", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7172", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5f53a85-583a-454f-a57f-1bfb3a148ced", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7171, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007171__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7172-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7172, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7173, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7494", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7173", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b54ad5db-114e-428f-91d3-2d38eb296d0e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7172, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007172__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7173-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7173, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7174, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7495", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7174", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ede119d4-421d-4f20-a210-8ba86d6d6373", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7173, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007173__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7174-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7174, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7175, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7496", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7175", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0adf61e8-da98-42ac-98db-658311dab38e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7174, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007174__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7175-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7175, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7176, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7497", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7176", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8543f89-b3b4-4206-80c8-c993c2bfaa6f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7175, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007175__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7176-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7176, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7177, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7498", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7177", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8d0d9e6-5c96-43fc-a388-60df3e403c05", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7176, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007176__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7177-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7177, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7178, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7499", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7178", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "51a52012-cb36-481c-85eb-c715969b8332", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7177, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007177__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7178-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7178, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7179, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7500", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7179", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e841f18b-fa55-49da-b1fd-ea8b6e3b5965", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 622908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7178, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007178__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7179-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7179, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7180, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7501", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7180", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "22f2515d-366d-4aa9-8540-3b2071ffaeec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7179, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007179__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7180-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7180, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7181, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7502", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7181", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d10372a-9ffa-4e18-ab86-ecae89320066", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7180, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007180__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7181-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7181, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "622953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7182, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7503", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7182", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f68529db-2a8d-4c4a-b64d-49109769f577", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7181, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007181__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7182-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7182, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7183, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:08 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7504", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7183", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2468e8f-9fea-4e93-88cb-bffb79b510a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7182, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007182__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7183-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7183, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7184, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7505", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7184", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "99939da6-32e7-4e4f-b56a-23326e414346", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7183, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007183__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7184-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7184, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7185, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7506", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7185", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd810a4c-8c31-40f1-be75-862fdde78562", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7184, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007184__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7185-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7185, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7186, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7507", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7186", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13cb795f-d3ae-43ce-ab5d-30b815770bb3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7185, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007185__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7186-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7186, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7187, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7508", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7187", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbf50f03-1821-4a3f-896b-062a32cd2a41", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7186, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007186__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7187-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7187, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7188, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7509", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7188", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36ed863d-da29-4002-801a-f68efc5227eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7187, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007187__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7188-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7188, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7189, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7510", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7189", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b29ff0bc-f4fd-4b1c-96be-61a946c7e276", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7188, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007188__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7189-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7189, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7190, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7511", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7190", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "afdc9610-c8b1-49d1-a425-515de2eb6a46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7189, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007189__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7190-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7190, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7191, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7512", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7191", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab26cdf4-3ea4-4c91-9f0b-40f4dc1b9255", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 623955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7190, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007190__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7191-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7191, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7192, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7513", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7192", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d4ad6769-bd20-48e0-8f21-2946e90ebdbd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7191, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007191__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7192-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7192, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "623906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7193, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7514", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7193", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ec3e193a-c4a0-4fea-bac4-bbe6bdd104d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7192, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007192__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7193-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7193, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7194, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7515", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7194", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b07fd960-7a54-45f9-8324-41f9efca4ddf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7193, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007193__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7194-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7194, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7195, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:09 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7516", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7195", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9986fdab-8d78-4da9-892e-d8dfed6d2bbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7194, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007194__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7195-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7195, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7196, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7517", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7196", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1dd91620-d81e-4cc7-be78-df356e863622", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7195, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007195__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7196-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7196, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7197, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7518", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7197", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20e20d3b-02a4-41a0-b523-beab5831ced7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7196, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007196__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7197-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7197, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7198, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7519", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7198", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2db08c1c-9507-42be-b01e-5435893cf200", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7197, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007197__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7198-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7198, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7199, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7520", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7199", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf8d996c-956c-4c02-b4dd-04c83eb072e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7198, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007198__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7199-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7199, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7200, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7521", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7200", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e40f988a-07c7-4bc1-bc6c-f4c6110c6c91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7199, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007199__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7200-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7200, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7201, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7522", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7201", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6814126-0133-4db9-a02a-17c0eab41dbb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7200, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007200__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7201-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7201, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7202, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7523", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7202", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fa4e77f-f1a7-4fe3-9460-ed2dfc53d728", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 624908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7201, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007201__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7202-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7202, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7203, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7524", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7203", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "96594b8f-02c9-4c21-b3be-9e9b7799b3a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7202, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007202__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7203-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7203, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7204, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7525", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7204", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06c104a4-e895-47d1-aa20-6b5ac16d694d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7203, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007203__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7204-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7204, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "624953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7205, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7526", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7205", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e35c4b0e-81f4-4dfd-a9b7-17123504829b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7204, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007204__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7205-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7205, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7206, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:10 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7527", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7206", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4bbcb398-5c69-4297-b22d-cdfdfd5d935d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7205, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007205__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7206-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7206, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7207, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7528", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7207", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "13227a89-837a-48b0-85f0-382d73e94018", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7206, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007206__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7207-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7207, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7208, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7529", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7208", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2464efd3-dd10-4b18-9d97-fb59a763538c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7207, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007207__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7208-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7208, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7209, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7530", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7209", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43b4441a-0375-484a-a779-9206a6fc125a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7208, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007208__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7209-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7209, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7210, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7531", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7210", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c8e6e61-c9a3-4473-a6cf-a72510d943c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7209, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007209__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7210-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7210, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7211, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7532", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7211", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c395300-641c-41ae-9b5c-e1447713b78c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7210, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007210__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7211-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7211, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7212, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7533", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7212", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3990dac5-30b6-41fc-a364-d7b3ebbfd9be", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7211, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007211__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7212-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7212, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7213, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7534", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7213", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df0b9511-27a4-4663-b2ab-e2e8c8d42400", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7212, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007212__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7213-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7213, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7214, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7535", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7214", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "084987cd-fc48-473b-9241-7b639a2a3b7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 625955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7213, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007213__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7214-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7214, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7215, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7536", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7215", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75678501-fd2d-4d62-ac94-31e3d2ddfdf0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7214, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007214__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7215-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7215, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7216, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7537", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7216", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98ae9d98-469f-4963-9e48-e50ab35f6ce1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7215, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007215__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7216-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7216, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "625984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7217, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7538", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7217", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b5643a1f-ee40-4538-a9fc-408ddc6b9bff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7216, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007216__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7217-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7217, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7218, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:11 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7539", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7218", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b01bf82-32e1-4d05-8a08-977b6145779f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7217, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007217__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7218-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7218, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7219, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7540", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7219", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "76167d04-150c-474c-9d27-0f36c09ae901", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7218, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007218__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7219-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7219, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7220, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7541", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7220", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "02de5fbe-16c2-44a7-b76a-76ab8eb74120", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7219, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007219__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7220-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7220, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7221, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7542", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7221", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9bfd1bb6-2c28-4ab6-83b3-349526f75892", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7220, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007220__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7221-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7221, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7222, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7543", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7222", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "edbf70cc-72d3-47f0-b09b-de29c9176518", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7221, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007221__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7222-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7222, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7223, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7544", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7223", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47a20a46-52d7-4514-8d9c-1b786809accb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7222, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007222__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7223-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7223, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7224, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7545", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7224", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "66d3b624-db2a-40e1-b94f-ce61a119473a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7223, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007223__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7224-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7224, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7225, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7546", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7225", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8b77c44-561c-418d-8e36-4aeebee0db10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7224, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007224__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7225-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7225, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7226, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7547", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7226", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8799aac0-2f2a-48a5-b8ad-71428436841c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7225, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007225__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7226-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7226, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7227, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7548", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7227", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31ab4b72-ba02-40b9-9b8d-3465a62fb7f1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7226, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007226__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7227-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7227, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "626937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7228, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7549", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7228", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98d0dddc-b485-4c04-bd79-5f0a0d4a2a19", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7227, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007227__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7228-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7228, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7229, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:12 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7550", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7229", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d28694e-87c0-4c7f-8efa-3e626a27a30d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7228, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007228__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7229-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7229, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7230, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7551", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7230", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8fcb1416-7091-4969-a2e1-bc307d7c9d43", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7229, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007229__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7230-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7230, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7231, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7552", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7231", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6a45df6-6c9e-4f40-ac6d-a10efaadac92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7230, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007230__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7231-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7231, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7232, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7553", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7232", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fda1f3b6-06e2-4c8d-a123-b576a86351f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7231, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007231__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7232-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7232, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7233, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7554", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7233", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8df6459a-6157-4f09-bd47-5d542cac049d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7232, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007232__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7233-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7233, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7234, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7555", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7234", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d11aa66b-56a5-4550-b2a2-8ab960c67588", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7233, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007233__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7234-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7234, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7235, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7556", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7235", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43033666-2619-4c57-82da-233cb15123cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7234, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007234__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7235-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7235, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7236, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7557", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7236", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "53afc319-ecb8-4b34-98f3-e324fd395b36", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7235, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007235__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7236-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7236, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7237, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7558", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7237", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4916ecf4-d795-4d39-bb1f-3341de2ae1b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 627940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7236, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007236__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7237-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7237, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7238, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7559", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7238", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6b07b26-406e-4710-a9e6-a5fbe6da5938", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7237, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007237__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7238-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7238, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7239, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7560", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7239", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5777d060-40d3-48af-9de2-7bd8a5a0a7c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7238, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007238__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7239-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7239, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "627984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7240, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7561", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7240", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "64483215-7671-4983-9820-7f40ab559156", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7239, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007239__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7240-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7240, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7241, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:13 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7562", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7241", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b98a9657-0f1b-42d9-8273-d10483efd2f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7240, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007240__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7241-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7241, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7242, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7563", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7242", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "444b2536-9fdf-4f21-87ff-0d695a118b22", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7241, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007241__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7242-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7242, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7243, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7564", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7243", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "968eaec2-5e14-42b2-9412-ff90febd8a4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7242, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007242__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7243-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7243, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7244, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7565", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7244", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "035fd8ef-4a05-4933-8210-4220e393d84d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7243, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007243__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7244-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7244, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7245, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7566", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7245", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5243d3c-6d30-470f-956d-063da5f576bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7244, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007244__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7245-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7245, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7246, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7567", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7246", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43319271-fa52-4c42-afb0-4129e70027a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7245, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007245__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7246-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7246, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7247, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7568", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7247", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e12baf1-48fa-466e-b78c-e6ca73fd41c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7246, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007246__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7247-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7247, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7248, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7569", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7248", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "326b942c-8e02-4d58-b173-e59099635713", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7247, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007247__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7248-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7248, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7249, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7570", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7249", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68844297-6b30-44b0-833e-2ad183d5ac07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 628986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7248, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007248__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7249-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7249, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7250, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7571", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7250", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6fd5119c-0c8b-4afd-8a85-612702a82234", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7249, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007249__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7250-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7250, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "628937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7251, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7572", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7251", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84504fc5-76b2-455a-bda1-35af7170f77a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7250, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007250__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7251-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7251, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7252, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:14 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7573", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7252", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48f6104c-30dc-4356-b8ab-29b270f51956", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7251, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007251__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7252-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7252, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7253, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7574", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7253", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57d214ea-63d1-408c-a91c-640704d20206", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7252, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007252__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7253-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7253, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7254, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7575", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7254", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc10f3dd-0607-473f-982c-097ad6f3febb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7253, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007253__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7254-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7254, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7255, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7576", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7255", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b6a35288-fa69-43c1-a47b-0a78785fb92f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7254, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007254__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7255-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7255, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7256, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7577", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7256", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "16ccd243-aebb-48f7-8952-d4a86b01317a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7255, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007255__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7256-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7256, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7257, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7578", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7257", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a3c578a-2c65-40c0-bdf0-39b167983560", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7256, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007256__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7257-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7257, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7258, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7579", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7258", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3c1566f8-6ea1-49d4-a16c-ba651b77cce0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7257, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007257__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7258-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7258, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7259, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7580", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7259", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cbba00f5-4b10-4bb6-a299-1f0348cb8900", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7258, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007258__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7259-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7259, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7260, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7581", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7260", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a95b631d-9e1c-4408-9e22-e8839b7cfbae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 629940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7259, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007259__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7260-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7260, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7261, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7582", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7261", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f835df3b-72a8-48b8-8f49-fcf294c1b884", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7260, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007260__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7261-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7261, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7262, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7583", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7262", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a42ad4d-a57d-4b3f-a01a-17467cd3292b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7261, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007261__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7262-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7262, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "629984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7263, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7584", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7263", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5fc2e2c6-7ce0-473b-856c-bda2563897a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7262, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007262__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7263-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7263, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7264, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:15 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7585", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7264", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d5dfd73-1ab5-4506-8171-d5ebc1cc1aea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7263, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007263__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7264-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7264, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7265, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7586", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7265", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81ba849c-20fa-4039-980f-f80b413f7e66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7264, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007264__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7265-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7265, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7266, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7587", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7266", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "62ec10c6-9ee7-4342-98d8-5a72b0682d99", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7265, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007265__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7266-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7266, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7267, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7588", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7267", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8f9f546-432b-46a8-9300-a01a67fe64cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7266, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007266__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7267-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7267, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7268, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7589", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7268", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "448c7804-b39e-4df5-aeaf-141e51bdc743", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7267, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007267__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7268-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7268, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7269, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7590", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7269", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0aa1d0d8-ec14-4ec0-8acd-5b87c808a092", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7268, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007268__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7269-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7269, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7270, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7591", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7270", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "55bcc6bf-e69f-407b-9246-c5caf3a88833", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7269, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007269__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7270-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7270, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7271, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7592", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7271", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "97d8ac62-2d3e-4c89-bcc6-a07ff689c28d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7270, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007270__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7271-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7271, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7272, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7593", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7272", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cde3189-4527-4387-b9ea-e26863a5444e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 630986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7271, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007271__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7272-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7272, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7273, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7594", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7273", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eba4df39-bb92-4985-92da-c4fb8667f7a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7272, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007272__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7273-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7273, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "630937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7274, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7595", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7274", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c23f5a13-f7e3-4f96-95dc-c14c5a422538", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7273, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007273__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7274-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7274, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7275, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:16 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7596", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7275", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6cc45002-d5ef-4990-89d0-9ace862a44d2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7274, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007274__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7275-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7275, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7276, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7597", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7276", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "157646f9-2d97-4913-8b55-5d030892ed4d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7275, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007275__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7276-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7276, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7277, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7598", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7277", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d1b46ce-7fde-431c-96cd-fb94d089bdeb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7276, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007276__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7277-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7277, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7278, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7599", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7278", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "234a4292-76b1-431d-ae04-a550721fa1e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7277, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007277__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7278-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7278, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7279, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7600", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7279", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "962f71a3-1f47-4d1e-a27c-f5ecd5b733e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7278, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007278__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7279-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7279, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7280, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7601", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7280", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "055b74c2-c76c-4b32-95cb-d8e8d835b59a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7279, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007279__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7280-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7280, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7281, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7602", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7281", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4c7695b-c5ff-4280-bdb0-5f48070b9c9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7280, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007280__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7281-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7281, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7282, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7603", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7282", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73d5856b-edb7-4647-96dc-c28091e389a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7281, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007281__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7282-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7282, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7283, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7604", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7283", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0543efc1-9f10-4920-8a49-a038ac47194b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 631940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7282, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007282__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7283-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7283, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7284, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7605", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7284", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "234e4449-ca5f-4b93-84f1-f11645c4f506", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7283, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007283__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7284-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7284, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7285, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7606", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7285", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb2d2428-81ec-46e4-be20-72dc30163612", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7284, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007284__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7285-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7285, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "631968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7286, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7607", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7286", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "332dcb07-d203-4f5d-8680-9544d6ceabe7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7285, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007285__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7286-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7286, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7287, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:17 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7608", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7287", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58fdd8af-81c3-4978-9f3c-d2bea3f815cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7286, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007286__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7287-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7287, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7288, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7609", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7288", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dc312c9-cf54-4a2b-822b-b2d4759b3e7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7287, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007287__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7288-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7288, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7289, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7610", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7289", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9af4f206-dbc4-46d7-9d36-c36b1f22adde", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7288, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007288__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7289-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7289, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7290, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7611", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7290", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b20a7434-f5ec-4c4a-811e-ddc284da0af7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7289, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007289__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7290-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7290, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7291, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7612", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7291", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2df40475-6baa-41d5-aa57-8349d2c1686c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7290, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007290__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7291-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7291, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7292, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7613", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7292", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "06a23d80-d1fb-4801-a930-2754fa40b8f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7291, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007291__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7292-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7292, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7293, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7614", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7293", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c98866a-eba7-425b-a2f6-0240005af855", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7292, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007292__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7293-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7293, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7294, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7615", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7294", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "124c547e-2b12-4a57-b401-c872f4c7e211", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7293, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007293__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7294-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7294, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7295, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7616", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7295", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "106f1d48-d80b-4f46-b648-653f7abebb2d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 632971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7294, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007294__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7295-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7295, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7296, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7617", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7296", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f39d5723-045e-409a-8eb0-a92c92753347", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7295, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007295__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7296-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7296, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "632921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7297, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7618", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7297", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c908b06b-bd75-4675-ab89-99b5fb0791f8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7296, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007296__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7297-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7297, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7298, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:18 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7619", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7298", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6688b2e4-bcbc-4be4-b90c-316d592b54d7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7297, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007297__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7298-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7298, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7299, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7620", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7299", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4e8bfe1-0628-4cc4-8a6a-d34cd5b6d0df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7298, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007298__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7299-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7299, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7300, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7621", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7300", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "71c5c7c1-4be3-4422-aeeb-a94adaf17e72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7299, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007299__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7300-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7300, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7301, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7622", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7301", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9ebb64c3-c551-4c45-bad6-ba34d769ae60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7300, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007300__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7301-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7301, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7302, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7623", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7302", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cd7336f3-782c-4d81-b208-56c244627126", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7301, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007301__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7302-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7302, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7303, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7624", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7303", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9c32e798-e8de-4aff-8a72-43b5a72d1cb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7302, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007302__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7303-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7303, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7304, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7625", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7304", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19c37d2c-ceaa-4ae8-a2cc-73c888a848fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7303, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007303__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7304-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7304, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7305, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7626", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7305", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a574577-07f2-49a2-b8c2-eb871c0c3a23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7304, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007304__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7305-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7305, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7306, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7627", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7306", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "442edda2-69e5-407e-b89c-ee3448ee5ea2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 633924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7305, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007305__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7306-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7306, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7307, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7628", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7307", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c34650d-086f-4cd7-bc7b-4afc59128f33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7306, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007306__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7307-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7307, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7308, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7629", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7308", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73681909-ab5c-4c59-a741-6827556f566d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7307, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007307__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7308-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7308, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "633968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7309, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7630", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7309", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6ccf167-610f-4b5b-9203-451d4d276571", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7308, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007308__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7309-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7309, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7310, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:19 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7631", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7310", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1725fddc-4ebb-45c4-88b6-eb2473c5ec6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7309, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007309__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7310-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7310, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7311, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7632", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7311", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d63d36ff-78af-4709-b177-19dbaad0bd57", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7310, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007310__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7311-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7311, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7312, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7633", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7312", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cdebf7f8-c835-4afb-80c2-88d292bbc7b0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7311, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007311__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7312-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7312, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7313, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7634", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7313", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0da96beb-0278-46e8-aba8-775a852cda4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7312, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007312__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7313-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7313, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7314, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7635", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7314", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "031ba1af-dc91-427b-9187-9092da091aa1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7313, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007313__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7314-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7314, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7315, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7636", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7315", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ffcfd63-a4b3-4f21-b8e0-dc0b3c4468d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7314, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007314__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7315-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7315, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7316, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7637", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7316", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cbcacf0-51b7-4609-9dbf-d6947d7b58b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7315, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007315__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7316-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7316, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7317, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7638", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7317", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7107887-ede2-4e11-9a0d-bed72828fcb1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7316, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007316__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7317-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7317, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7318, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7639", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7318", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "979a25db-24a7-4ef7-90e6-8bbb7fb19fff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 634971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7317, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007317__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7318-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7318, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7319, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7640", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7319", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc5e485a-c510-468f-9ca8-a01d1c9b624e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7318, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007318__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7319-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7319, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "634921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7320, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7641", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7320", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b67caad9-36c1-413e-8280-adac6207c455", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7319, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007319__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7320-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7320, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7321, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7642", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7321", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "916ed57a-aa6a-4d35-b221-c656cd7c3389", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7320, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007320__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7321-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7321, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7322, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7643", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7322", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1e6d28f-38ba-40b3-a409-e07b6d0bab06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7321, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007321__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7322-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7322, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7323, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7644", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7323", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19bf77ed-b443-40a6-b3c6-45f1674a1a98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7322, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007322__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7323-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7323, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7324, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7645", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7324", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db52d173-8857-49af-a27e-83004ad3564f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7323, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007323__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7324-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7324, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7325, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7646", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7325", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0fcb77f6-5543-4fa3-8534-d32baefc624f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7324, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007324__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7325-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7325, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7326, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7647", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7326", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "20577c43-1b5f-47bc-880b-a427fcd744fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7325, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007325__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7326-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7326, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7327, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7648", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7327", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "38fb852a-7eda-4342-a2e8-aed953ab90ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7326, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007326__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7327-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7327, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7328, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7649", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7328", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d71ae964-8116-486d-af6c-05637b8f8ea8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7327, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007327__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7328-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7328, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7329, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7650", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7329", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb193e2b-d39f-4b14-909b-b1b9d3909fd3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 635924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7328, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007328__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7329-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7329, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7330, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7651", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7330", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f28caab-626f-4711-84bd-2bd98aac8f14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7329, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007329__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7330-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7330, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7331, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7652", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7331", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3530839-b10d-420c-b708-9a6b29ee3245", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7330, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007330__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7331-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7331, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "635968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7332, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7653", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7332", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b01389c8-6846-41a6-bb95-76279948b5b1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7331, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007331__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7332-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7332, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7333, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7654", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7333", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af59fafb-959d-4ebe-8b04-157d2ce70580", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7332, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007332__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7333-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7333, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7334, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7655", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7334", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88d1de8f-3850-4074-9f77-e761f4814e7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7333, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007333__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7334-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7334, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7335, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7656", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7335", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1f7c837-bf8d-49f3-b372-6a58f91939ca", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7334, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007334__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7335-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7335, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7336, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7657", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7336", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7672d18-8eaa-4c71-ae48-91e1f1ea7192", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7335, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007335__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7336-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7336, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7337, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7658", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7337", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfc39f8c-fecd-4c15-aed6-8448b87456cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7336, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007336__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7337-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7337, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7338, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7659", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7338", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28c8c686-1267-4f92-8d6b-966799dd47d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7337, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007337__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7338-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7338, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7339, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7660", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7339", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "45a383b0-ec36-491e-a9c1-529456bd01fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7338, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007338__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7339-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7339, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7340, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7661", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7340", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "39e7e954-e92d-43f7-aa25-ceccea886c7d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7339, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007339__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7340-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7340, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7341, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7662", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7341", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2cdea3c4-279f-4486-aa64-d733b75ff7d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 636971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7340, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007340__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7341-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7341, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7342, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7663", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7342", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2b97357-f877-489a-99ed-6877f18c1d72", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7341, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007341__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7342-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7342, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "636921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7343, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7664", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7343", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9db8ca32-3a08-4204-befc-aaea5d8bab2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7342, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007342__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7343-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7343, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7344, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7665", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7344", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81e625a5-4476-41e7-9136-cfe53e538168", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7343, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007343__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7344-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7344, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7345, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7666", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7345", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e4d5a6a9-e986-49ca-9d70-e34d33e4f65a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7344, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007344__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7345-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7345, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7346, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7667", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7346", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6afc51b1-0c97-4a0d-9c4d-3ce496e03470", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7345, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007345__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7346-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7346, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7347, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7668", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7347", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9bf60136-5a37-4885-a4b3-4342d71fcc3b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7346, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007346__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7347-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7347, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7348, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7669", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7348", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61ba0b55-7d55-4072-bb42-5a9d466c6396", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7347, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007347__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7348-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7348, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7349, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7670", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7349", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "80558d78-96da-427d-84b6-15273138ed10", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7348, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007348__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7349-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7349, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7350, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7671", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7350", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "feaa84cd-7aac-4aac-9d63-4aa50bba85bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7349, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007349__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7350-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7350, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7351, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7672", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7351", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65d19f68-7257-4f6e-89ec-f16c4f7eee56", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7350, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007350__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7351-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7351, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637703.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7352, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7673", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7352", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce447a2c-f790-48c4-a4a8-41efb1844c18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 637924.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7351, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007351__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7352-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7352, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7353, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7674", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7353", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72848d7c-99b1-448e-810f-de99d9c5f5a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7352, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007352__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7353-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7353, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637875.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7354, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7675", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7354", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "945d6750-d7fa-438e-93ae-3772a767cb7c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638096.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7353, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007353__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7354-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7354, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "637953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7355, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7676", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7355", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b50b9adc-8341-4ba7-9eab-f1fdbc623a6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7354, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007354__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7355-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7355, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7356, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7677", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7356", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "92043057-2b9a-45ac-9ea6-a96009f6deb3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7355, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007355__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7356-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7356, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7357, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7678", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7357", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7aa90fa0-b6c7-429f-9e4c-ece5d9dd3e68", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7356, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007356__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7357-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7357, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7358, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7679", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7358", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d73dcb44-428b-4f2d-9262-7a28240bbb6b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7357, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007357__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7358-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7358, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638312.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7359, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7680", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7359", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "67ddad27-7284-48dd-b2d0-18b3a64b912b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638533.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7358, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007358__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7359-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7359, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7360, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7681", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7360", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "392ff7b7-f545-42c9-9fe0-b097388bac3a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7359, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007359__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7360-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7360, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638484.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7361, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7682", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7361", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b17ebf2-5b69-481f-a673-e2c056d64bc4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638705.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7360, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007360__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7361-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7361, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7362, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7683", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7362", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de604160-a27a-48ea-b290-13bfabcae323", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7361, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007361__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7362-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7362, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638656.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7363, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7684", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7363", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4e0d426a-a578-40ea-9218-7f31b3edf9b2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638877.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7362, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007362__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7363-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7363, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7364, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7685", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7364", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "846a589d-e275-472d-a935-3497f4d5ba06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7363, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007363__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7364-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7364, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7365, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7686", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7365", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb72be73-e301-4eef-981c-ef6719ff6bfb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7364, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007364__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7365-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7365, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "638906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7366, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7687", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7366", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3dd0badc-8008-44ae-9397-c7ffd82479c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7365, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007365__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7366-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7366, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7367, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7688", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7367", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3bdf8ad3-c2b9-42b7-a73c-eaece46ac34a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7366, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007366__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7367-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7367, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639093.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7368, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7689", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7368", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a9b5f7e-126b-4ba6-a148-f9e3c0085dcb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639315, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7367, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007367__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7368-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7368, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7369, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7690", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7369", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0ebb3e98-1c31-4b39-8d3e-f303de2aa01b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7368, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007368__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7369-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7369, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639265.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7370, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7691", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7370", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d40de0e8-46c2-4ebd-a692-60ff10d42b63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639486.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7369, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007369__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7370-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7370, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7371, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7692", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7371", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e34248b2-1a33-424a-82ed-82804db85d81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7370, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007370__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7371-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7371, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639437.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7372, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7693", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7372", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7100a587-5688-4acf-808d-da67278d04ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639658.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7371, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007371__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7372-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7372, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7373, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7694", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7373", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e1cdc795-9db2-42ee-b106-9f60a2b24bad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7372, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007372__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7373-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7373, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7374, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7695", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7374", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6effef3d-1de6-4e7d-b34b-1acd7582871c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7373, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007373__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7374-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7374, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7375, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7696", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7375", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a5d8366b-49e2-49c1-b306-3adb58035858", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 639908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7374, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007374__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7375-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7375, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7376, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7697", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7376", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0a1ce7b3-bcc3-4536-b3e2-7cc9bad74204", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7375, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007375__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7376-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7376, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7377, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7698", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7377", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f2d06ab-7bdd-418e-ad7d-c1fcde8ccf2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7376, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007376__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7377-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7377, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "639953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7378, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7699", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7378", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f80f68e0-aa05-4201-84ac-5c34c141ceea", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7377, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007377__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7378-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7378, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640046.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7379, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7700", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7379", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8779f996-9e7a-4692-8430-66ea310052d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640268.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7378, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007378__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7379-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7379, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7380, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7701", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7380", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8b319e3-b4d7-4a4b-8903-d6c28654756b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7379, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007379__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7380-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7380, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640218.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7381, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7702", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7381", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1784fc32-bec2-45c5-8e5e-3d7774b660f9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640440, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7380, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007380__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7381-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7381, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7382, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7703", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7382", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "179510c2-53fe-437a-bcbb-0f9c8bcc0e80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7381, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007381__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7382-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7382, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640390.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7383, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7704", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7383", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9a00d50f-c7ef-4fca-b61f-3d37c060017d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640611.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7382, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007382__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7383-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7383, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7384, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7705", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7384", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bf08e8af-2444-43c1-a4d0-e18322527fcc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7383, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007383__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7384-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7384, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7385, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7706", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7385", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fb0959d0-319a-453f-9d3b-58fe8ef55f81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7384, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007384__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7385-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7385, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7386, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7707", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7386", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9baaa67-599a-41a7-a0c6-29c1225e1096", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7385, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007385__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7386-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7386, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7387, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7708", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7387", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8e0c0c6-9f79-49d5-8f71-e7c6275aa486", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 640955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7386, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007386__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7387-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7387, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640828.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7388, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7709", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7388", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "40f1eee6-d770-4b0e-8fb2-063ec1c5678c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641049.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7387, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007387__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7388-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7388, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "640906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7389, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7710", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7389", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8f7db906-4e37-42bb-8bf4-481f2a89b604", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7388, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007388__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7389-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7389, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641000.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7390, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7711", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7390", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ecdb3e81-8295-4d5f-9a4e-a388e45051fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641221.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7389, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007389__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7390-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7390, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7391, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:26 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7712", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7391", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fd38dd2a-6205-4005-a2fa-c1b51362b768", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7390, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007390__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7391-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7391, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641171.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7392, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7713", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7392", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2514f0f4-8dab-4251-8cf3-fbe4615b8a09", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641393.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7391, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007391__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7392-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7392, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7393, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7714", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7393", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ceb1c282-e8ab-446d-96e4-a03b5cc6161e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7392, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007392__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7393-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7393, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7394, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7715", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7394", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dd333a5-7fdf-427a-86ae-64bdec955ddb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7393, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007393__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7394-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7394, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7395, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7716", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7395", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1e2a8b47-206e-4ac3-b65b-8c04bedb9fb7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7394, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007394__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7395-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7395, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7396, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7717", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7396", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d0141e9-b9ae-4d39-a6a1-ff98313a1251", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7395, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007395__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7396-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7396, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641609.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7397, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7718", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7397", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d0f315ef-1350-4847-8acb-1da05f1db5f7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641830.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7396, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007396__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7397-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7397, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7398, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7719", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7398", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d4a2dd5-36ca-4327-91f4-8ec5cde97266", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 641908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7397, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007397__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7398-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7398, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641781.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7399, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7720", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7399", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ef948183-eb62-4aeb-bce0-146a7c9fd148", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642002.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7398, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007398__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7399-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7399, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7400, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7721", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7400", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0f618191-a0b7-462d-ba5c-0d3a012a3637", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7399, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007399__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7400-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7400, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "641953.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7401, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7722", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7401", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0675f97a-ec5d-4c0b-a957-5dfa4bdaa9bc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642174.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7400, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007400__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7401-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7401, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7402, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:27 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7723", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7402", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ea4ad84-3ffa-4508-83ce-c5c22956e8ad", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7401, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007401__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7402-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7402, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642125.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7403, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7724", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7403", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3aa92b44-781f-4fe3-ac73-d04727b72a27", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642346.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7402, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007402__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7403-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7403, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7404, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7725", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7404", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ffd5cda3-2d65-49ca-a013-1be0050783a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7403, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007403__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7404-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7404, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7405, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7726", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7405", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8ba16400-9a66-4ee7-b10f-f754aea167e6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7404, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007404__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7405-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7405, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7406, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7727", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7406", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27e10674-3f73-411c-8538-e4aca039a592", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7405, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007405__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7406-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7406, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7407, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7728", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7407", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1b4b7b9-f6b3-4c24-b286-e35279fcd1bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7406, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007406__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7407-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7407, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642562.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7408, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7729", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7408", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eff259f6-3ec1-41ac-b20a-44b944d9ad9b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642783.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7407, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007407__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7408-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7408, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7409, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7730", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7409", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b2d4d141-21f2-4576-8301-05a5aaae81c2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7408, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007408__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7409-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7409, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642734.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7410, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7731", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7410", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4c75567f-623f-4226-bbe2-eaf10fb65c02", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 642955.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7409, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007409__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7410-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7410, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7411, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7732", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7411", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ccf1333-e1e9-46db-a0a3-393d502b4a64", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7410, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007410__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7411-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7411, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642906.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7412, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7733", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7412", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "540135c9-3edb-45cb-b793-1e0ff6b2bff6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643127.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7411, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007411__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7412-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7412, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "642984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7413, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7734", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7413", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc9fd15b-0d95-4c16-8e1e-fa979ccd8148", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7412, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007412__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7413-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7413, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7414, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:28 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7735", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7414", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "46f93262-275d-4046-984e-0fbb6e8f8625", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7413, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007413__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7414-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7414, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7415, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7736", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7415", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3f16ab48-1c26-4e18-a71e-db8e716c0ab7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7414, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007414__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7415-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7415, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7416, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7737", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7416", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0070a595-4010-43d4-8c69-93d4da5b6e63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7415, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007415__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7416-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7416, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643343.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7417, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7738", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7417", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f513bdd0-9eda-4df8-a07c-d1a5c7b9faeb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643565, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7416, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007416__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7417-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7417, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7418, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7739", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7418", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0696e590-4a0e-4555-b367-80d29c1dd1c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7417, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007417__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7418-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7418, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643515.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7419, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7740", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7419", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09ed9992-9233-44ba-914b-27a70d0dac7f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643736.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7418, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007418__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7419-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7419, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7420, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7741", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7420", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18f4f109-0ff2-417e-a05d-d552a1f92ab0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7419, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007419__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7420-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7420, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643687.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7421, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7742", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7421", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "09507f40-a2f6-423e-8e3f-622c192be9e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643908.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7420, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007420__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7421-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7421, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7422, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7743", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7422", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e428d543-859a-41d1-8a5d-38f961f30890", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 643986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7421, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007421__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7422-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7422, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7423, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7744", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7423", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e87c3fc-e890-40cb-9d6a-8d571a4ffbba", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7422, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007422__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7423-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7423, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "643937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7424, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7745", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7424", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "81d93ecf-e8d2-4e87-a4b8-e32b380caef7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7423, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007423__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7424-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7424, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7425, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:29 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7746", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7425", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89028202-465a-4a0f-ab8d-3e23cacd32a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7424, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007424__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7425-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7425, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7426, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7747", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7426", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fbd946db-a9e5-41c2-a0c4-963b6643c926", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7425, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007425__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7426-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7426, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7427, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7748", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7427", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4cbb2b9c-de6e-4ce7-8cf5-75acfa00b198", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7426, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007426__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7427-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7427, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644296.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7428, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7749", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7428", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "885b3c90-06d1-4f41-b579-de8cf6f1f6a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644518.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7427, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007427__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7428-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7428, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7429, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7750", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7429", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58723bcf-e66d-4abf-a2dd-e5409eda6af0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7428, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007428__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7429-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7429, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644468.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7430, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7751", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7430", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4269a1e5-5344-4855-95e7-a6b8384337a0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644690, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7429, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007429__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7430-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7430, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7431, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7752", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7431", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "73272504-b407-4271-9201-46aa3e1ee870", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7430, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007430__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7431-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7431, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644640.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7432, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7753", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7432", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cc92e2f1-6df0-4ccc-9591-5cf212a8c6ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644861.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7431, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007431__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7432-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7432, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7433, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7754", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7433", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e129c8d3-cf34-4e93-89c2-c6768f1a01c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 644940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7432, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007432__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7433-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7433, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7434, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7755", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7434", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "36290178-44f9-400d-aaa0-922d4625ae65", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7433, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007433__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7434-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7434, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7435, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7756", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7435", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1f2d5352-9a99-4ab9-8f4c-30498852ad81", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7434, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007434__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7435-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7435, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "644984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7436, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7757", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7436", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6e89e77-947f-44c7-9fbf-48c5954629cb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7435, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007435__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7436-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7436, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645078.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7437, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:30 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7758", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7437", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f49f8ff2-0515-4095-8589-f020f673f108", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645299.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7436, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007436__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7437-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7437, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7438, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7759", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7438", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47b68390-98d4-4dd3-a1b5-20810c922cec", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7437, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007437__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7438-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7438, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645250.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7439, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7760", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7439", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "066e378b-6bdc-4c22-86cd-f450b119ad66", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645471.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7438, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007438__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7439-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7439, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7440, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7761", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7440", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ec6e90a-1b24-47cc-8a4d-117c09503dd1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7439, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007439__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7440-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7440, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645421.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7441, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7762", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7441", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5338e1df-1679-48a6-bd82-8998bdaaed1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645643.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7440, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007440__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7441-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7441, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7442, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7763", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7442", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "61042246-ef76-47fd-b23e-629f8094e27e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7441, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007441__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7442-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7442, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7443, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7764", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7443", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "345f563b-d63a-433f-9834-6d151d9a1991", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7442, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007442__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7443-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7443, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7444, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7765", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7444", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fad6a88b-1401-4217-8174-0c6e6bcfddf1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7443, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007443__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7444-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7444, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7445, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7766", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7445", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e8b3d42-7492-4b44-9817-47dc3d38bcff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 645986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7444, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007444__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7445-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7445, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645859.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7446, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7767", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7446", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cf31b6a3-2309-47fb-81c5-927062256b8b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646080.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7445, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007445__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7446-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7446, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "645937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7447, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7768", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7447", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56da454b-4468-41ed-8dd2-0a3612eb05d8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7446, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007446__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7447-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7447, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646031.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7448, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7769", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7448", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b0f66e51-0a7a-482a-821f-0aa5e0d4732c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646252.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7447, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007447__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7448-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7448, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7449, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7770", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7449", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e089891-db11-4a17-aa32-6585e28bc419", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7448, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007448__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7449-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7449, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646203.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7450, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7771", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7450", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57142937-b7f0-49c9-8703-3e1cffe0bed4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646424.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7449, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007449__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7450-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7450, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7451, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7772", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7451", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "86d6641e-5c46-4f8f-81bd-1dacf6540229", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7450, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007450__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7451-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7451, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646375.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7452, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7773", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7452", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1a88f01-06c4-4f58-adb2-752cb60b378e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646596.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7451, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007451__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7452-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7452, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7453, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7774", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7453", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5548a424-2b5c-4bff-b8d6-06ec08c01614", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7452, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007452__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7453-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7453, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7454, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7775", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7454", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "30b378df-62b0-49a8-a2b1-29b28b53dcc7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7453, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007453__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7454-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7454, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7455, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7776", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7455", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d594968-247e-4b95-be0f-b8864b1ea04e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7454, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007454__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7455-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7455, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7456, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7777", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7456", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2a5636d-ff7e-4c0a-86b8-c2dcee57645e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 646940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7455, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007455__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7456-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7456, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646812.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7457, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7778", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7457", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8b67fae4-d90a-4cc3-917a-7a75d985b9fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647033.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7456, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007456__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7457-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7457, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7458, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7779", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7458", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31234872-c3f1-4ef0-80f2-38e5484e81b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7457, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007457__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7458-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7458, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "646984.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7459, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7780", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7459", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "af7f758f-33fd-41e2-8025-af815a254af9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647205.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7458, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007458__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7459-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7459, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7460, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7781", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7460", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae3d5745-8ac2-4b16-ad08-cbf4441bd7df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7459, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007459__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7460-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7460, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647156.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7461, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7782", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7461", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50969492-9cd0-447b-a491-1521a059ab5c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647377.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7460, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007460__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7461-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7461, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7462, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7783", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7462", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42593c81-755c-49d9-bc94-981206321158", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7461, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007461__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7462-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7462, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7463, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7784", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7463", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c995c714-8fe5-47a1-890b-8f0b9a655ab0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7462, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007462__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7463-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7463, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7464, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7785", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7464", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a793d897-289d-4ff9-a329-4ce8308a4731", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7463, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007463__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7464-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7464, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7465, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7786", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7465", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3cefcdfc-aeb7-43e9-81bf-8a8c5135a20b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7464, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007464__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7465-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7465, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647593.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7466, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7787", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7466", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "24efcc70-4d37-4c6b-bf5d-1b754c55bc4f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7465, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007465__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7466-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7466, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7467, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7788", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7467", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e8b7e6fb-78c5-4626-b5c3-f86ec055c9df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7466, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007466__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7467-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7467, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647765.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7468, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7789", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7468", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34ca4031-6ca4-4471-900b-05793df7d2d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 647986.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7467, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007467__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7468-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7468, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7469, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7790", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7469", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c7db98c9-d1ab-4a5d-8d9e-4a2acf1ee3a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7468, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007468__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7469-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7469, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "647937.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7470, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7791", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7470", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b62ad5e-ae1c-4486-90b5-b385b9abbd80", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648158.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7469, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007469__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7470-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7470, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7471, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7792", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7471", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b45e0fd9-4a7d-45d0-8e95-7d75b158ad4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7470, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007470__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7471-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7471, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7472, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7793", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7472", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a0f1942f-684a-46d9-aac5-32387d43e5e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7471, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007471__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7472-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7472, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7473, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7794", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7473", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "beb9d72a-165e-4425-b16a-97eb591805ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7472, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007472__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7473-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7473, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7474, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7795", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7474", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0631ab69-1dd9-4d37-a1d3-c000d01d7d60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7473, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007473__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7474-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7474, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7475, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7796", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7475", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dd6f029f-bf8f-4bea-aa44-36c7dd69da13", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7474, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007474__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7475-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7475, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7476, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7797", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7476", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e3a6794-2ae8-44fb-ae8e-bc861793c8bb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7475, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007475__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7476-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7476, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648546.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7477, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7798", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7477", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae10961a-09e2-4415-bca0-cdaef0c385cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648768.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7476, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007476__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7477-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7477, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648625.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7478, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7799", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7478", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df753b76-1533-46f6-9ccd-1eaddff4fe0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648846.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7477, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007477__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7478-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7478, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648718.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7479, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7800", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7479", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "90e99597-b65c-4feb-8643-817f4991acdb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 648940, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7478, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007478__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7479-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7479, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648796.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7480, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7801", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7480", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f37cd794-8247-459d-b99e-c214cfeb4397", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649018.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7479, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007479__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7480-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7480, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648890.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7481, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7802", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7481", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d5a61597-325e-4afd-8f0b-ffd0c8250c11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649111.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7480, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007480__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7481-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7481, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "648968.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7482, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7803", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7482", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7923ffaa-3052-493c-9119-255db04e47eb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649190, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7481, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007481__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7482-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7482, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649062.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7483, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7804", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7483", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d568f884-0316-4376-8810-f1cdde88c6b8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649283.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7482, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007482__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7483-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7483, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649140.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7484, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7805", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7484", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "04a287ee-5fb8-4807-bdee-458291163bd9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649361.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7483, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007483__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7484-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7484, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649234.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7485, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7806", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7485", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4bac1cc-af0f-44c3-b931-edbb0d023dfa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649455.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7484, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007484__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7485-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7485, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649328.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7486, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7807", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7486", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9589d119-9065-41ff-97a1-6c31e7618d45", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649549.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7485, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007485__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7486-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7486, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649406.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7487, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7808", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7487", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6be8a7b-57ba-4d0c-a285-c96217daf301", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649627.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7486, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007486__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7487-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7487, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649500.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7488, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7809", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7488", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "abd59ba1-d963-4933-89ab-3c866da7bb42", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649721.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7487, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007487__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7488-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7488, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649578.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7489, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7810", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7489", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "65f2125d-15ce-4ba1-aabb-58535b156815", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649799.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7488, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007488__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7489-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7489, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649671.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7490, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7811", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7490", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dba7203d-d83a-4f95-8721-c7798a599e35", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649893.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7489, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007489__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7490-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7490, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649750.00", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7491, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7812", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7491", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a446759-f72b-4b80-be33-a5fc110de25c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 649971.25, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7490, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007490__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7491-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7491, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649843.75", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7492, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7813", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7492", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83a6f899-f005-4bcc-8304-9885e25f1644", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650065, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7491, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007491__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7492-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7492, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "649921.88", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7493, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7814", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7493", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3b20ec7-1ccf-4b50-a393-af6fccbdcc1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650143.1199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7492, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007492__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7493-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7493, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "650015.63", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7494, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7815", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7494", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "23c95872-100c-4084-b42f-7407aff3bf2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650236.8699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7493, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007493__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7494-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7494, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "650109.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7495, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7816", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7495", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6d855ac4-3535-43fb-b22b-08d9b7f0990c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650330.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7494, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007494__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7495-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7495, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "650187.50", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7496, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7817", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7496", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b9935f46-fbe8-4de5-baa5-b7053fd054d6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650408.75, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7495, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007495__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7496-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7496, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "650281.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7497, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7818", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7497", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1987c3ad-9e5d-4aca-9bef-9d88f8692f0c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650502.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7496, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007496__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7497-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7497, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "650359.38", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7498, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7819", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7498", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "535da855-073c-4430-a1fe-bb22584204b5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650580.6199951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7497, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007497__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7498-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7498, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "650453.13", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7499, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7820", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7499", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4b94a29-9f7e-4b26-b2cb-ca9c11e3b730", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650674.3699951172, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7498, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007498__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null }, "FrameKey-7499-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 7499, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "quantEM", "quantEM-ShutterOpenDelay": "0", "quantEM-ElapsedTime-ms": "650531.25", "quantEM-StartTime-ms": "458342325890.63", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": null, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-29", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 7500, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-10 21:29:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "ImageNumber": "7821", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-ImageNumber": "7500", "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "956c5223-b411-4160-bdba-331aa2d058e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 650752.5, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 7499, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": "512", "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": "512", "Summary": { "Slices": 1, "Interval_ms": 0, "UUID": "81082b5b-c4f9-4b7d-b085-fc2caad1c39f", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-10 21:18:45 -0400", "Date": "2014-07-10", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Bacon24729_ROI-02_Rhod3", "PixelSize_um": 0, "Frames": 7500, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-10_Bacon24729_MEA_Rhod-3\\Bacon24729_ROI-02" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000007499__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": null } }